CN107022592A - A kind of method that low dose irradiation extracts collagen in fish-skin - Google Patents
A kind of method that low dose irradiation extracts collagen in fish-skin Download PDFInfo
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- CN107022592A CN107022592A CN201710168091.3A CN201710168091A CN107022592A CN 107022592 A CN107022592 A CN 107022592A CN 201710168091 A CN201710168091 A CN 201710168091A CN 107022592 A CN107022592 A CN 107022592A
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 53
- 108010035532 Collagen Proteins 0.000 title claims abstract description 53
- 229920001436 collagen Polymers 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 50
- 239000000284 extract Substances 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 102000004190 Enzymes Human genes 0.000 claims abstract description 49
- 108090000790 Enzymes Proteins 0.000 claims abstract description 49
- 238000005238 degreasing Methods 0.000 claims abstract description 30
- 230000000694 effects Effects 0.000 claims abstract description 24
- 230000001925 catabolic effect Effects 0.000 claims abstract description 16
- 239000002781 deodorant agent Substances 0.000 claims abstract description 14
- 235000013305 food Nutrition 0.000 claims abstract description 14
- 108090001060 Lipase Proteins 0.000 claims abstract description 12
- 239000000796 flavoring agent Substances 0.000 claims abstract description 12
- 235000019634 flavors Nutrition 0.000 claims abstract description 12
- 239000006260 foam Substances 0.000 claims abstract description 12
- 235000013372 meat Nutrition 0.000 claims abstract description 12
- 150000001875 compounds Chemical class 0.000 claims abstract description 11
- 239000012141 concentrate Substances 0.000 claims abstract description 9
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 229940088598 enzyme Drugs 0.000 claims description 47
- 239000011521 glass Substances 0.000 claims description 21
- 239000000463 material Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- GUTLYIVDDKVIGB-OUBTZVSYSA-N Cobalt-60 Chemical compound [60Co] GUTLYIVDDKVIGB-OUBTZVSYSA-N 0.000 claims description 15
- 239000000706 filtrate Substances 0.000 claims description 15
- 238000001694 spray drying Methods 0.000 claims description 14
- 239000000843 powder Substances 0.000 claims description 13
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 230000005855 radiation Effects 0.000 claims description 10
- 206010013786 Dry skin Diseases 0.000 claims description 8
- 241000251468 Actinopterygii Species 0.000 claims description 7
- 241000522254 Cassia Species 0.000 claims description 5
- 239000009636 Huang Qi Substances 0.000 claims description 5
- 235000007265 Myrrhis odorata Nutrition 0.000 claims description 5
- 102000057297 Pepsin A Human genes 0.000 claims description 5
- 108090000284 Pepsin A Proteins 0.000 claims description 5
- 240000004760 Pimpinella anisum Species 0.000 claims description 5
- 235000012550 Pimpinella anisum Nutrition 0.000 claims description 5
- 102000005158 Subtilisins Human genes 0.000 claims description 5
- 108010056079 Subtilisins Proteins 0.000 claims description 5
- 235000006886 Zingiber officinale Nutrition 0.000 claims description 5
- 238000000889 atomisation Methods 0.000 claims description 5
- 238000011010 flushing procedure Methods 0.000 claims description 5
- 235000008397 ginger Nutrition 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 235000012907 honey Nutrition 0.000 claims description 5
- 229940111202 pepsin Drugs 0.000 claims description 5
- 235000019991 rice wine Nutrition 0.000 claims description 5
- 235000021419 vinegar Nutrition 0.000 claims description 5
- 239000000052 vinegar Substances 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 244000273928 Zingiber officinale Species 0.000 claims 1
- 239000003595 mist Substances 0.000 claims 1
- 239000007921 spray Substances 0.000 claims 1
- 238000003756 stirring Methods 0.000 claims 1
- 230000004048 modification Effects 0.000 abstract description 8
- 238000012986 modification Methods 0.000 abstract description 8
- 238000010521 absorption reaction Methods 0.000 abstract description 6
- 238000005260 corrosion Methods 0.000 abstract description 4
- 230000007062 hydrolysis Effects 0.000 abstract description 4
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000002779 inactivation Effects 0.000 abstract 1
- 108010028690 Fish Proteins Proteins 0.000 description 6
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 4
- 108010000912 Egg Proteins Proteins 0.000 description 4
- 241000234314 Zingiber Species 0.000 description 4
- 235000014103 egg white Nutrition 0.000 description 4
- 210000000969 egg white Anatomy 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 230000003321 amplification Effects 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 210000002468 fat body Anatomy 0.000 description 3
- 230000008676 import Effects 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 235000020995 raw meat Nutrition 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 241000252234 Hypophthalmichthys nobilis Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000252230 Ctenopharyngodon idella Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 210000004087 cornea Anatomy 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000000774 hypoallergenic effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
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- 229920000642 polymer Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 210000003786 sclera Anatomy 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
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- General Health & Medical Sciences (AREA)
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- Gastroenterology & Hepatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to food processing technology field, disclose a kind of method that low dose irradiation extracts collagen in fish-skin, main process is that fish-skin is made into fish-skin meat foam, fish-skin feed liquid is made after being soaked in deodorant solution, then glycerol ester hydrolase, food flavor enzyme and compound catabolic enzyme are added and is placed in irradiation in 60Coradiation, concentrate is made in the heated inactivation of feed liquid after irradiation, filtering, decolouring, concentration, concentrate is spray-dried collagen is made.The method that the low dose irradiation of the present invention extracts collagen is irradiated by applying in enzymolysis, skimming processes to feed liquid, enzymolysis speed can be improved, hydrolysis time is reduced, and strengthens degreasing effect, the yield and quality of collagen is improved, and can effectively kill microorganism, the anti-corrosion modification effect of collagen is lifted, strengthens the preservativity of collagen, and the strand of collagen shortens, mean molecule quantity is 1500~3500 dalton, is more suitable for absorption of human body.
Description
Technical field
The present invention relates to food processing technology field, and in particular to a kind of low dose irradiation extracts collagen in fish-skin
Method.
Background technology
Collagen be distributed widely in the skin of animal, bone, connective tissue, tendon, cornea, gut cell interstitial, flesh chamber,
The positions such as sclera, because with good biocompatibility, biodegradable and bioactivity, in food, medicine, tissue work
The fields such as journey, cosmetics obtain extensive use.Make aquatic livestock especially with the difference in terms of the degree of cross linking because amino acid is constituted
It is that contained abundant collagen in processing waste-skin, bone, squama has many livestock collagens unexistent excellent
Point, such as low antigenicity, hypoallergenic characteristic.While collagen content is also above terrestrial animal, such as silver carp in aquatic livestock body
In the fish-skin of fish, bighead and grass carp protein content be respectively collagen content in 25.9%, 23.6% and 29.8%, and fish-skin most
Height can exceed the 80% of fish protein total amount, and more other positions are much higher.But fish-skin etc. is frequent in existing fish are processed
Thrown away as processing waste or leftover bits and pieces, cause the wasting of resources.
The existing method for preparing Fish protein has alkali extraction method, enzyme process etc., and wherein alkali extraction method easily makes amino acid be damaged, hydrolyze
Degree is whard to control and the aesthetic quality of product and nutritive validity are undesirable.Enzyme process mainly uses proteolytic enzyme processing former
Material hydrolysis, mild condition and hydrolysis degree is easy to control, but fishlike smell remove not exclusively, in Fish protein containing the influence of faint bitter taste
The mouthfeel of Fish protein, Fish protein yield are low, and fat content is high, and Fish protein quality is not high, and fresh keeping time is short.
Chinese patent CN201610594281, a kind of preparation method of sole collagen powder of patent name, the applying date
Disclose a kind of method that utilization mixed enzymolysis method prepares sole collagen powder July 26 2016 phase, but collagen egg
White yield is low, fat content is high, and with easy infection microorganism, fresh keeping time is short.
The content of the invention
For extracting the problem of prior art of collagen has collagen yield and low quality, this hair from fish-skin
Bright purpose is that providing a kind of yield that can effectively improve collagen and the low dose irradiation of quality extracts glue in fish-skin
The method of former albumen, the high income of collagen, and fresh keeping time is long, it is of good preservation effect.
The present invention provides following technical scheme：
A kind of method that low dose irradiation extracts collagen in fish-skin, comprises the following steps：
（1）Take the fish-skin leftover bits and pieces after fresh fish-skin or flesh of fish processing to be blended after being rinsed well through water and fish-skin meat foam is made；
（2）Fish-skin meat foam is placed in 10~30 DEG C of deodorant solution and soaked 4~6 hours, the dry fish-skin that is made is done after flushing
Powder；
（3）It is 45%~55% fish-skin material that water is added into fish-skin dry powder and adjusts pH value mass concentration is made to 5.5~7.5
Liquid；
（4）Fish-skin feed liquid is moved into clear glass cup and glycerol ester hydrolase and food flavor enzyme is added, the irradiation field of cobalt -60 is subsequently placed in
In irradiated, degreasing, defishying degreasing feed liquid is made；
（5）Compound catabolic enzyme is added in degreasing feed liquid into clear glass cup, continuation irradiation degreasing feed liquid, which is decomposed, is made enzymolysis
Material；
（6）Heating enzymolysis material 45~60 minutes, then through 300~400 mesh sieve net filtrations, retain filtrate at 85~95 DEG C；
（7）Filtrate is handled through activated carbon decolorizing, then filtered, and concentration is made through being concentrated in vacuo to the 20%~30% of original volume
Liquid；
（8）Concentrate is delivered into spray drying device spray drying collagen is made.
It is used as a modification of the present invention, step（2）In deodorant solution be prepared from through procedure below：By parts by weight
10~15 parts of the Radix Astragali, 8~12 parts of Chinese cassia tree, 4.5~5.5 parts of ginger, 6~8 parts of anise seed are placed in the water of 400~500 parts by weight
Boil, then filter, 12~16 parts of 50~80 parts of 50~80 parts of vinegar, yellow rice wine, the honey of parts by weight is added into filtrate, is stirred
Mix and deodorant solution uniformly is made.
It is used as a modification of the present invention, step（4）Irradiance method be：Clear glass cup is high by 50~60 apart from ground
Cm, apart from the m of the irradiation bomb of cobalt -60 1.2~1.6, points of four sections times irradiation, every section of exposure time 30~45 seconds is spaced 45 minutes,
Irradiance rate is followed successively by 10 Gy/s, 20 Gy/s, 30 Gy/s, 40 Gy/s, and irradiation nonuniformity is less than 1.2, radiation environment temperature
14~20 DEG C.
It is used as a modification of the present invention, step（4）The addition of middle glycerol ester hydrolase is the 6 ‰ of fish-skin feed liquid quality
~9 ‰, enzyme activity is 20,000~30,000 U/g, and the addition of food flavor enzyme is 3 ‰~the 6 ‰ of fish-skin feed liquid quality, and enzyme activity is 1.2
Ten thousand~1.8 ten thousand U/g.
It is used as a modification of the present invention, step（5）Irradiance method be：Clear glass cup is high by 50~60 apart from ground
Cm, apart from the m of the irradiation bomb of cobalt -60 1.2~1.6, points of four sections times irradiation, every section of exposure time 30~45 seconds is spaced 45 minutes,
Irradiance rate is followed successively by 6 Gy/s, 12 Gy/s, 18 Gy/s, 24 Gy/s, and irradiation nonuniformity is less than 1.2, radiation environment temperature 14
~20 DEG C.
It is used as a modification of the present invention, step（5）In be combined catabolic enzyme addition for degreasing feed liquid quality 4 ‰~
8 ‰, enzyme activity is 50,000~70,000 U/g, and the compound catabolic enzyme is by trypsase, pepsin and subtilopeptidase A group
Into its mass ratio is 1:0.8~1.2:1.5~2.0.
As a modification of the present invention, the inlet temperature of spray drying device is 170~185 DEG C, and outlet temperature is 70
~85 DEG C, the MPa of atomisation pressure 0.4~0.6.
The low dose irradiation of the present invention extracts the method for collagen in fish-skin, and that fish-skin is made into fish-skin meat foam is molten in deodorant
Soak, then the fish-skin feed liquid and degreasing feed liquid in enzymolysis process are irradiated, the heated enzyme that goes out of the feed liquid after irradiation in liquid
Collagen protein powder is made in activity, concentration, vacuum drying spraying.After absorbing irradiation ray molecule activation occurs for feed liquid in irradiation process
And free radical is produced, Free Radical causes molecule chain break in polymer substances such as protein, promotes the high scores such as protein
The degraded of sub- material, so as to form small molecular protein, improves degradation rate, shortens enzymolysis time, improve the easy of collagen
Degree of absorption, and the oxidation removal of fat and aliphatic acid in skimming processes is promoted, so as to improve the yield of collagen
And quality, while fully lifting glycerol ester hydrolase, food flavor enzyme and Compound Water using the method for increasing irradiance rate step by step in irradiation
The activity of enzyme is solved, promotes degreasing, deodorant and the degradation process of feed liquid.In addition irradiation ray can effectively kill degreasing, deodorant with
And the microorganism in degradation process, the anti-corrosion for improving collagen goes bad ability, extends the fresh keeping time of collagen and fresh-keeping
Effect.And Flavonoid substances, the antioxidant contained in deodorant solution etc. can strengthen the bactericidal effect of irradiation, collagen egg is lifted
The rotten ability of white anti-corrosion.
Beneficial effects of the present invention are as follows：
The method that the low dose irradiation of the present invention extracts collagen is irradiated by applying in enzymolysis, skimming processes to feed liquid,
Enzymolysis speed can be improved, hydrolysis time is reduced, and strengthens degreasing effect, the yield and quality of collagen, Er Qieneng is improved
It is enough effectively to kill microorganism, the anti-corrosion modification effect of collagen is lifted, strengthens the preservativity of collagen, while collagen egg
White strand shortens, and is more suitable for absorption of human body.
Embodiment
Unless otherwise instructed, the raw material employed in the present invention is commercially available or commonly used in the art, such as
Without special instruction, the method in following embodiments is the conventional method of this area.
Embodiment 1
A kind of method that low dose irradiation extracts collagen in fish-skin, comprises the following steps：
（1）Take the fish-skin leftover bits and pieces after fresh fish-skin or flesh of fish processing to be blended after being rinsed well through water and fish-skin meat foam is made；
（2）Fish-skin meat foam is placed in 10 DEG C of deodorant solution and soaked 4 hours, fish-skin dry powder is made in drying after flushing, wherein de-
Raw meat solution is preferably prepared from through procedure below：The g of the Radix Astragali 10, the g of Chinese cassia tree 8, the g of ginger 4.5, the g of anise seed 6 are placed in 400 g
Water in boil, then filter, vinegar 50 g, the g of yellow rice wine 50, the g of honey 12 added into filtrate and is stirred；
（3）Water is added to fish-skin dry powder and the pH value of solution is adjusted, and the fish-skin feed liquid that mass concentrations are 45% is made to 5.5；
（4）Fish-skin feed liquid is moved into clear glass cup and glycerol ester hydrolase and food flavor enzyme is added, the irradiation field of cobalt -60 is subsequently placed in
In irradiated, degreasing, defishying degreasing feed liquid is made, the addition of glycerol ester hydrolase is the 6 ‰ of fish-skin feed liquid quality, enzyme
Vigor is 30,000 U/g, and the addition of food flavor enzyme is the 3 ‰ of fish-skin feed liquid quality, and enzyme activity is 1.8 ten thousand U/g, wherein irradiance method
For：Clear glass cup is apart from high 50 cm in ground, apart from the m of the irradiation bomb of cobalt -60 1.2, point four sections of time irradiation, every section of exposure time
30 seconds, it is spaced 45 minutes, irradiance rate is followed successively by 10 Gy/s, 20 Gy/s, 30 Gy/s, 40 Gy/s, and irradiation nonuniformity is less than
1.2,14 DEG C of radiation environment temperature；
（5）Compound catabolic enzyme is added in degreasing feed liquid into clear glass cup, continuation irradiation degreasing feed liquid, which is decomposed, is made enzymolysis
Material, the addition for being combined catabolic enzyme is the 4 ‰ of degreasing feed liquid quality, and enzyme activity is 70,000 U/g, and compound catabolic enzyme is preferably by pancreas egg
White enzyme, pepsin and subtilopeptidase A composition, its mass ratio is 1:0.8:1.5, wherein irradiance method is：Clear glass
Cup is high by 50 apart from ground, apart from the m of the irradiation bomb of cobalt -60 1.2, points of four sections times irradiation, every section of exposure time 30 seconds, is spaced 45 points
Clock, irradiance rate is followed successively by 6 Gy/s, 12 Gy/s, 18 Gy/s, 24 Gy/s, and irradiation nonuniformity is less than 1.2, radiation environment temperature
Spend for 14 DEG C；
（6）By step（5）Enzymatic activity is eliminated in gained enzymolysis material heating, and preferably heating-up temperature is 85 DEG C, 45 minutes heat times,
Then 300 mesh sieve net filtrations enzymolysis material, retains filtrate；
（7）Filtrate is handled through activated carbon decolorizing, then filtered, and 20% through being concentrated in vacuo to original volume is made concentrate；
（8）Concentrate is delivered into spray drying device spray drying collagen, the import temperature of preferably spray drying equipment is made
Spend for 170 DEG C, outlet temperature is 70 DEG C, the MPa of atomisation pressure 0.4.
Method through the present invention extracts protein content >=96.5% in the collagen prepared, and mean molecule quantity is
1500~3500 dalton, suitable absorption of human body, fat content≤0.096%, and in 23 ± 2 DEG C, relative humidity 50 ± 5%
Light protected environment in the clump count amplification that is detected after 12 months of sealing preserve -1 ‰~3 ‰, it is of good preservation effect.
Embodiment 2
A kind of method that low dose irradiation extracts collagen in fish-skin, comprises the following steps：
（1）Take the fish-skin leftover bits and pieces after fresh fish-skin or flesh of fish processing to be blended after being rinsed well through water and fish-skin meat foam is made；
（2）Fish-skin meat foam is placed in 20 DEG C of deodorant solution and soaked 5 hours, fish-skin dry powder is made in drying after flushing, wherein de-
Raw meat solution is preferably prepared from through procedure below：The g of the Radix Astragali 12.5, the g of Chinese cassia tree 10, the g of ginger 5, the g of anise seed 7 are placed in 450 g
Water in boil, then filter, vinegar 65 g, the g of yellow rice wine 65, the g of honey 14 added into filtrate and is stirred；
（3）The fish-skin feed liquid that mass concentration is 50% is made to 6.5 in the pH value for adding water to fish-skin dry powder and adjusting solution；
（4）Fish-skin feed liquid is moved into clear glass cup and glycerol ester hydrolase and food flavor enzyme is added, the irradiation field of cobalt -60 is subsequently placed in
In irradiated, degreasing, defishying degreasing feed liquid is made, the addition of glycerol ester hydrolase is the 7.5 ‰ of fish-skin feed liquid quality,
Enzyme activity is 1.5 ten thousand U/g, and the addition of food flavor enzyme is the 4.5 ‰ of fish-skin feed liquid quality, and enzyme activity is 1.6 ten thousand U/g, wherein spoke
It is according to method：Clear glass cup is apart from high 50~60 cm in ground, apart from the m of the irradiation bomb of cobalt -60 1.2~1.6, interradius when dividing four sections
According to, every section of exposure time 37.5 seconds is spaced 45 minutes, irradiance rate is followed successively by 10 Gy/s, 20 Gy/s, 30 Gy/s, 40 Gy/s,
Irradiation nonuniformity is less than 1.2, keeps 17 DEG C of radiation environment temperature；
（5）Compound catabolic enzyme is added in degreasing feed liquid into clear glass cup, continuation irradiation degreasing feed liquid, which is decomposed, is made enzymolysis
Material, the addition for being combined catabolic enzyme is the 6 ‰ of degreasing feed liquid quality, and enzyme activity is 60,000 U/g, and compound catabolic enzyme is preferably by pancreas egg
White enzyme, pepsin and subtilopeptidase A composition, its mass ratio is 1:1.0:1.75, wherein irradiance method is：Transparent glass
Glass cup is apart from high 55 cm in ground, apart from the m of the irradiation bomb of cobalt -60 1.4, points of four sections times irradiation, every section of exposure time 37.5 seconds,
Every 45 minutes, irradiance rate was followed successively by 6 Gy/s, 12 Gy/s, 18 Gy/s, 24 Gy/s, and irradiation nonuniformity is less than 1.2, keeps
17 DEG C of radiation environment temperature；
（6）By step（5）Enzymatic activity is eliminated in gained enzymolysis material heating, and preferably heating-up temperature is 90 DEG C, 52.5 points of heat time
Clock, then 350 mesh sieve net filtrations enzymolysis material, retains filtrate；
（7）Filtrate is handled through activated carbon decolorizing, then filtered, and 25% through being concentrated in vacuo to original volume is made concentrate；
（8）Concentrate is delivered into spray drying device spray drying collagen, the import temperature of preferably spray drying equipment is made
Spend for 177.5 DEG C, outlet temperature is 77.5 DEG C, the MPa of atomisation pressure 0.5.
Method through the present invention extracts protein content >=96.5% in the collagen prepared, and mean molecule quantity is
1500~3500 dalton, suitable absorption of human body, fat content≤0.096%, and in 23 ± 2 DEG C, relative humidity 50 ± 5%
Light protected environment in the clump count amplification that is detected after 12 months of sealing preserve -1 ‰~3 ‰, it is of good preservation effect.
Embodiment 3
A kind of method that low dose irradiation extracts collagen in fish-skin, comprises the following steps：
（1）Take the fish-skin leftover bits and pieces after fresh fish-skin or flesh of fish processing to be blended after being rinsed well through water and fish-skin meat foam is made；
（2）Fish-skin meat foam is placed in 30 DEG C of deodorant solution and soaked 6 hours, fish-skin dry powder is made in drying after flushing, wherein de-
Raw meat solution is preferably prepared from through procedure below：The g of the Radix Astragali 15, the g of Chinese cassia tree 12, the g of ginger 5.5, the g of anise seed 8 are placed in 500 g
Water in boil, then filter, vinegar 80 g, the g of yellow rice wine 80, the g of honey 16 added into filtrate and is stirred；
（3）The fish-skin feed liquid that mass concentration is 55% is made to 7.5 in the pH value for adding water to fish-skin dry powder and adjusting solution；
（4）Fish-skin feed liquid is moved into clear glass cup and glycerol ester hydrolase and food flavor enzyme is added, the irradiation field of cobalt -60 is subsequently placed in
In irradiated, degreasing, defishying degreasing feed liquid is made, the addition of glycerol ester hydrolase is the 9 ‰ of fish-skin feed liquid quality, enzyme
Vigor is 20,000 U/g, and the addition of food flavor enzyme is the 6 ‰ of fish-skin feed liquid quality, and enzyme activity is 1.2 ten thousand U/g, wherein irradiance method
For：Clear glass cup is apart from high 60 cm in ground, apart from the m of the irradiation bomb of cobalt -60 1.6, point four sections of time irradiation, every section of exposure time
45 seconds, it is spaced 45 minutes, irradiance rate is followed successively by 10 Gy/s, 20 Gy/s, 30 Gy/s, 40 Gy/s, and irradiation nonuniformity is less than
1.2,20 DEG C of radiation environment temperature.
（5）Compound catabolic enzyme is added in degreasing feed liquid into clear glass cup, continuation irradiation degreasing feed liquid, which is decomposed, to be made
Enzymolysis material, be combined catabolic enzyme addition be degreasing feed liquid quality 8 ‰, enzyme activity be 50,000 U/g, compound catabolic enzyme preferably by
Trypsase, pepsin and subtilopeptidase A composition, its mass ratio is 1:1.2:2.0, wherein irradiance method is：It is transparent
Glass is apart from high 50~60 cm in ground, apart from the m of the irradiation bomb of cobalt -60 1.2~1.6, point four sections of time irradiation, during every section of irradiation
Between 45 seconds, be spaced 45 minutes, irradiance rate is followed successively by 6 Gy/s, 12 Gy/s, 18 Gy/s, 24 Gy/s, and irradiation nonuniformity is less than
1.2, it is 20 DEG C to keep radiation environment temperature；
（6）By step（5）Enzymatic activity is eliminated in gained enzymolysis material heating, and preferably heating-up temperature is 95 DEG C, 60 minutes heat times,
Then 400 mesh sieve net filtrations enzymolysis material, retains filtrate；
（7）Filtrate is handled through activated carbon decolorizing, then filtered, and concentration is made through being concentrated in vacuo to the 20%~30% of original volume
Liquid；
（8）Concentrate is delivered into spray drying device spray drying collagen, the import temperature of preferably spray drying equipment is made
Spend for 185 DEG C, outlet temperature is 85 DEG C, the MPa of atomisation pressure 0.6.
Method through the present invention extracts protein content >=96.5% in the collagen prepared, and mean molecule quantity is
1500~3500 dalton, suitable absorption of human body, fat content≤0.096%, and in 23 ± 2 DEG C, relative humidity 50 ± 5%
Light protected environment in the amplification of clump count that is detected after 12 months of sealing preserve be -1 ‰~3 ‰, it is of good preservation effect.
Claims (7)
1. a kind of method that low dose irradiation extracts collagen in fish-skin, comprises the following steps：
（1）Take the fish-skin leftover bits and pieces after fresh fish-skin or flesh of fish processing to be blended after being rinsed well through water and fish-skin meat foam is made；
（2）Fish-skin meat foam is placed in 10~30 DEG C of deodorant solution and soaked 4~6 hours, the dry fish-skin that is made is done after flushing
Powder；
（3）It is 45%~55% fish-skin material that water is added into fish-skin dry powder and adjusts pH value mass concentration is made to 5.5~7.5
Liquid；
（4）Fish-skin feed liquid is moved into clear glass cup and glycerol ester hydrolase and food flavor enzyme is added, the irradiation field of cobalt -60 is subsequently placed in
In irradiated, degreasing, defishying degreasing feed liquid is made；
（5）Compound catabolic enzyme is added in degreasing feed liquid into clear glass cup, continuation irradiation degreasing feed liquid, which is decomposed, is made enzymolysis
Material；
（6）Heating enzymolysis material 45~60 minutes, then through 300~400 mesh sieve net filtrations, retain filtrate at 85~95 DEG C；
（7）Filtrate is handled through activated carbon decolorizing, then filtered, and concentration is made through being concentrated in vacuo to the 20%~30% of original volume
Liquid；
（8）Concentrate is delivered into spray drying device spray drying collagen is made.
2. the method that a kind of low dose irradiation according to claim 1 extracts collagen in fish-skin, it is characterised in that step
Suddenly（2）In deodorant solution be prepared from through procedure below：By 10~15 parts of the Radix Astragali of parts by weight, 8~12 parts of Chinese cassia tree, ginger
4.5~5.5 parts, 6~8 parts of anise seed be placed in the water of 400~500 parts by weight and boil, then filter, weight added into filtrate
50~80 parts of the vinegar, 50~80 parts of yellow rice wine, 12~16 parts of honey of part, stir and deodorant solution are made.
3. the method that a kind of low dose irradiation according to claim 1 extracts collagen in fish-skin, it is characterised in that step
Suddenly（4）Irradiance method be：Clear glass cup is apart from high 50~60 cm in ground, apart from the m of the irradiation bomb of cobalt -60 1.2~1.6, point
Four times irradiation, every section of exposure time 30~45 seconds, is spaced 45 minutes, irradiance rate is followed successively by 10 Gy/s, 20 Gy/s, 30
Gy/s, 40 Gy/s, irradiation nonuniformity are less than 1.2,14~20 DEG C of radiation environment temperature.
4. the method that a kind of low dose irradiation according to claim 1 extracts collagen in fish-skin, it is characterised in that step
Suddenly（4）The addition of middle glycerol ester hydrolase is 6 ‰~the 9 ‰ of fish-skin feed liquid quality, and enzyme activity is 20,000~30,000 U/g, local flavor
The addition of enzyme is 3 ‰~the 6 ‰ of fish-skin feed liquid quality, and enzyme activity is 1.2 ten thousand~1.8 ten thousand U/g.
5. the method that a kind of low dose irradiation according to right wants 1 extracts collagen in fish-skin, it is characterised in that step
（5）Irradiance method be：Clear glass cup is apart from high 50~60 cm in ground, apart from the m of the irradiation bomb of cobalt -60 1.2~1.6, divides four
Section time irradiation, every section of exposure time 30~45 seconds is spaced 45 minutes, irradiance rate is followed successively by 6 Gy/s, 12 Gy/s, 18 Gy/
S, 24 Gy/s, irradiation nonuniformity are less than 1.2,14~20 DEG C of radiation environment temperature.
6. the method that a kind of low dose irradiation according to claim 1 extracts collagen in fish-skin, it is characterised in that step
Suddenly（5）In be combined catabolic enzyme addition be degreasing feed liquid quality 4 ‰~8 ‰, enzyme activity be 50,000~70,000 U/g, it is described multiple
Close catabolic enzyme to be made up of trypsase, pepsin and subtilopeptidase A, its mass ratio is 1:0.8~1.2:1.5~
2.0。
7. the method that a kind of low dose irradiation according to claim 1 extracts collagen in fish-skin, it is characterised in that spray
The inlet temperature of mist drying equipment is 170~185 DEG C, and outlet temperature is 70~85 DEG C, the MPa of atomisation pressure 0.4~0.6.
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|---|---|---|---|---|
| CN103773830A (en) * | 2014-01-21 | 2014-05-07 | 曾佳 | Method for extracting collagen from fish scales |
| CN103789381A (en) * | 2014-02-14 | 2014-05-14 | 钟春燕 | Method for rapidly extracting fish collagen |
| CN103829030A (en) * | 2014-03-27 | 2014-06-04 | 海南华研生物科技有限公司 | Method for producing low-fish-smell fish skin collagen |
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2017
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|---|---|---|---|---|
| CN103773830A (en) * | 2014-01-21 | 2014-05-07 | 曾佳 | Method for extracting collagen from fish scales |
| CN103789381A (en) * | 2014-02-14 | 2014-05-14 | 钟春燕 | Method for rapidly extracting fish collagen |
| CN103829030A (en) * | 2014-03-27 | 2014-06-04 | 海南华研生物科技有限公司 | Method for producing low-fish-smell fish skin collagen |
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| 傅俊杰: "《农产品辐照加工及检测》", 31 August 2013, 浙江大学出版社 * |
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