CN107022592B - Method for extracting collagen from fish skin by low-dose irradiation - Google Patents
Method for extracting collagen from fish skin by low-dose irradiation Download PDFInfo
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
Abstract
The invention relates to the technical field of food processing, and discloses a method for extracting collagen from fish skin by low-dose irradiation, which mainly comprises the steps of preparing fish skin into fish skin minced meat, soaking the fish skin minced meat in a deodorization solution to prepare fish skin feed liquid, then adding glyceride hydrolase, flavor enzyme and compound decomposition enzyme, placing the fish skin feed liquid in a cobalt-60 irradiation field for irradiation, heating and inactivating the irradiated feed liquid, filtering, decoloring and concentrating to prepare concentrated solution, and spraying and drying the concentrated solution to prepare the collagen. According to the method for extracting the collagen by low-dose irradiation, the irradiation is applied to the feed liquid in the enzymolysis and degreasing processes, so that the enzymolysis rate can be improved, the hydrolysis time can be shortened, the degreasing effect can be enhanced, the yield and the quality of the collagen can be improved, microorganisms can be effectively killed, the corrosion and deterioration effects of the collagen can be improved, the fresh-keeping capacity of the collagen can be enhanced, the molecular chain of the collagen is shortened, the average molecular weight is 1500-3500 daltons, and the collagen is more suitable for being absorbed by a human body.
Description
Technical Field
The invention relates to the technical field of food processing, in particular to a method for extracting collagen from fish skin by low-dose irradiation.
Background
Collagen is widely distributed in the skin, skeleton, connective tissue, tendon, cornea, visceral intercellular substance, muscle cavity, sclera and other parts of animals, and has good biocompatibility, biodegradability and bioactivity, so that the collagen is widely applied to the fields of food, medicine, tissue engineering, cosmetics and the like. Due to the difference in amino acid composition, crosslinking degree and the like, the abundant collagen contained in the skin, bone and scale which are waste products of aquatic animals, particularly processing, has the advantages which are not possessed by the collagen of livestock, such as low antigenicity, low irritability and the like. Meanwhile, the collagen content in the aquatic animal body is also higher than that in the skins of the terrestrial animals, such as chubs, bighead carps and grass carps, by 25.9 percent, 23.6 percent and 29.8 percent respectively, and the collagen content in the skins can exceed 80 percent of the total amount of the proteins of the fishes to a maximum extent and is much higher than that in other parts. However, fish skin and the like are often thrown away as processing waste or leftovers in the conventional fish processing, which causes resource waste.
The existing methods for preparing the fish protein comprise an alkali extraction method, an enzyme method and the like, wherein the alkali extraction method is easy to damage amino acid, the hydrolysis degree is difficult to control, and the sensory quality and the nutritional effect of the product are not ideal. The enzymatic method mainly utilizes proteolytic enzyme to treat raw materials for hydrolysis, the conditions are mild, the hydrolysis degree is easy to control, but the fishy smell is not completely removed, the fish protein contains weak bitter taste to influence the taste of the fish protein, the yield of the fish protein is low, the fat content is high, the quality of the fish protein is not high, and the preservation time is short.
Chinese patent CN201610594281, the patent name is a preparation method of plaice skin collagen powder, and application date 2016, 7, 26 discloses a method for preparing plaice skin collagen powder by using a mixed enzymatic hydrolysis method, but the yield of collagen is low, the fat content is high, and the fresh-keeping time is short because of the susceptibility of microorganisms.
Disclosure of Invention
Aiming at the problems of low collagen yield and low quality of the prior art for extracting collagen from fish skin, the invention aims to provide a method for extracting collagen from fish skin by low-dose irradiation, which can effectively improve the yield and the quality of the collagen, and has the advantages of high collagen yield, long preservation time and good preservation effect.
The invention provides the following technical scheme:
a method for extracting collagen from fish skin by low-dose irradiation comprises the following steps:
(1) washing fresh fish skin or fish skin leftovers after processing fish meat with water, and crushing to obtain fish skin meat powder;
(2) soaking the fish skin minced meat in a deodorization solution at the temperature of 10-30 ℃ for 4-6 hours, washing and drying to prepare fish skin dry powder;
(3) adding water into the dried fish skin powder and adjusting the pH value to 5.5-7.5 to prepare fish skin feed liquid with the mass concentration of 45-55%;
(4) transferring the fish skin feed liquid into a transparent glass cup, adding glyceride hydrolase and flavor enzyme, and then placing in a cobalt-60 irradiation field to prepare degreased feed liquid through irradiation, degreasing and deodorization treatment;
(5) adding compound decomposition enzyme into the degreased feed liquid in the transparent glass cup, and continuously irradiating the degreased feed liquid to decompose to prepare an enzymolysis material;
(6) heating the enzymolysis material at 85-95 ℃ for 45-60 minutes, filtering by using a 300-400 mesh screen, and keeping filtrate;
(7) decoloring the filtrate by active carbon, then filtering, and carrying out vacuum concentration to 20-30% of the original volume to prepare a concentrated solution;
(8) and (4) sending the concentrated solution to spray drying equipment for spray drying to obtain the collagen.
As an improvement of the invention, the fishy smell removing solution in the step (2) is prepared by the following processes: 10-15 parts of astragalus membranaceus, 8-12 parts of cinnamon, 4.5-5.5 parts of ginger and 6-8 parts of fennel seeds in parts by weight are placed in 400-500 parts of water to be boiled, then the mixture is filtered, 50-80 parts of edible vinegar, 50-80 parts of yellow wine and 12-16 parts of honey in parts by weight are added into the filtrate, and the mixture is uniformly stirred to prepare the fishy smell removing solution.
As an improvement of the invention, the irradiation method in the step (4) comprises the following steps: the transparent glass cup is 50-60 cm away from the ground surface, 1.2-1.6 m away from a cobalt-60 irradiation source, irradiation is carried out in four sections of time, the irradiation time of each section is 30-45 seconds, the interval is 45 minutes, the irradiation rates are 10Gy/s, 20Gy/s, 30Gy/s and 40Gy/s in sequence, the irradiation unevenness is less than 1.2, and the irradiation environment temperature is 14-20 ℃.
As an improvement of the invention, in the step (4), the addition of the glyceride hydrolase is 6-9 per thousand of the mass of the fish skin feed liquid, the enzyme activity is 2-3 ten thousand U/g, the addition of the flavor enzyme is 3-6 per thousand of the mass of the fish skin feed liquid, and the enzyme activity is 1.2-1.8 ten thousand U/g.
As an improvement of the invention, the irradiation method in the step (5) comprises the following steps: the transparent glass cup is 50-60 cm away from the ground surface, 1.2-1.6 m away from a cobalt-60 irradiation source, irradiation is carried out in four sections of time, the irradiation time of each section is 30-45 seconds, the interval is 45 minutes, the irradiation rates are 6Gy/s, 12Gy/s, 18Gy/s and 24Gy/s in sequence, the irradiation unevenness is less than 1.2, and the irradiation environment temperature is 14-20 ℃.
As an improvement of the invention, in the step (5), the addition amount of the compound decomposition enzyme is 4-8 per mill of the mass of the degreasing feed liquid, the enzyme activity is 5-7 ten thousand U/g, the compound decomposition enzyme is composed of trypsin, pepsin and subtilisin, and the mass ratio of the compound decomposition enzyme to the subtilisin is 1: 0.8-1.2: 1.5-2.0.
As an improvement of the invention, the inlet temperature of the spray drying equipment is 170-185 ℃, the outlet temperature is 70-85 ℃, and the spray pressure is 0.4-0.6 MPa.
The method for extracting collagen from fish skin by low-dose irradiation comprises the steps of soaking fish skin and minced meat prepared from fish skin in a deodorization solution, then irradiating fish skin feed liquid and degreasing feed liquid in an enzymolysis process, heating the irradiated feed liquid to inactivate enzyme, concentrating, drying in vacuum and spraying to prepare collagen powder. In the irradiation process, the feed liquid absorbs irradiation rays and then generates molecular activation to generate free radicals, the free radicals act on high molecular substances such as proteins to cause the breakage of molecular chains and promote the degradation of the high molecular substances such as the proteins, thereby forming small molecular proteins, improving the degradation rate, shortening the enzymolysis time, improving the easy absorption degree of the collagen, and promoting the oxidation and removal of fat and fatty acid in the degreasing process, thereby improving the yield and quality of the collagen, and simultaneously, the method of increasing the irradiation rate step by step is adopted in the irradiation to fully improve the activities of glyceride hydrolase, flavor enzyme and compound hydrolase and promote the degreasing, fishy smell removal and degradation processes of the feed liquid. In addition, the irradiation rays can effectively kill microorganisms in the processes of degreasing, deodorization and degradation, improve the antiseptic and deterioration capacity of the collagen, and prolong the preservation time and preservation effect of the collagen. And flavonoid substances, antioxidants and the like contained in the deodorization solution can enhance the sterilization effect of irradiation, and the anti-corrosion and deterioration capacity of the collagen is improved.
The invention has the following beneficial effects:
according to the method for extracting the collagen by the low-dose irradiation, the irradiation is applied to the feed liquid in the enzymolysis and degreasing processes, so that the enzymolysis rate can be improved, the hydrolysis time can be shortened, the degreasing effect can be enhanced, the yield and the quality of the collagen can be improved, microorganisms can be effectively killed, the corrosion and deterioration prevention effect of the collagen can be improved, the fresh-keeping capacity of the collagen can be enhanced, meanwhile, the molecular chain of the collagen is shortened, and the method is more suitable for being absorbed by a human body.
Detailed Description
The starting materials used in the present invention are commercially available or commonly used in the art, unless otherwise specified, and the methods in the following examples are conventional in the art, unless otherwise specified.
Example 1
A method for extracting collagen from fish skin by low-dose irradiation comprises the following steps:
(1) washing fresh fish skin or fish skin leftovers after processing fish meat with water, and crushing to obtain fish skin meat powder;
(2) soaking the minced fish skin meat in a fishy smell removing solution at 10 ℃ for 4 hours, washing and drying to prepare dried fish skin powder, wherein the fishy smell removing solution is prepared by the following processes: boiling 10g of astragalus, 8g of cinnamon, 4.5 g of ginger and 6g of fennel seed in 400 g of water, filtering, adding 50 g of edible vinegar, 50 g of yellow wine and 12g of honey into the filtrate, and uniformly stirring;
(3) adding water into the dried fish skin powder and adjusting the pH value of the solution to 5.5 to prepare fish skin feed liquid with the mass concentration of 45%;
(4) transferring the fish skin feed liquid into a transparent glass cup, adding glyceride hydrolase and flavor enzyme, then placing the fish skin feed liquid into a cobalt-60 irradiation field, and carrying out irradiation, degreasing and deodorization treatment to prepare degreased feed liquid, wherein the addition amount of the glyceride hydrolase is 6 per mill of the mass of the fish skin feed liquid, the enzyme activity is 3 ten thousand U/g, the addition amount of the flavor enzyme is 3 per mill of the mass of the fish skin feed liquid, and the enzyme activity is 1.8 ten thousand U/g, wherein the irradiation method comprises the following steps: the transparent glass cup is 50 cm away from the ground surface, 1.2 m away from a cobalt-60 irradiation source, irradiation is carried out in four sections of time, the irradiation time of each section is 30 seconds, the interval is 45 minutes, the irradiation rates are 10Gy/s, 20Gy/s, 30Gy/s and 40Gy/s in sequence, the irradiation unevenness is less than 1.2, and the irradiation environment temperature is 14 ℃;
(5) adding compound decomposition enzyme into the degreasing liquid in the transparent glass cup, continuously irradiating the degreasing liquid to decompose and prepare an enzymolysis material, wherein the adding amount of the compound decomposition enzyme is 4 per mill of the mass of the degreasing liquid, the enzyme activity is 7 ten thousand U/g, the compound decomposition enzyme is preferably composed of trypsin, pepsin and subtilisin, the mass ratio of the trypsin to the pepsin to the subtilisin is 1:0.8:1.5, and the irradiation method comprises the following steps: the transparent glass cup is 50 ℃ away from the ground surface and 1.2 m away from a cobalt-60 irradiation source, irradiation is carried out in four sections of time, the irradiation time of each section is 30 seconds, the interval is 45 minutes, the irradiation rates are 6Gy/s, 12Gy/s, 18Gy/s and 24Gy/s in sequence, the irradiation unevenness is less than 1.2, and the irradiation environment temperature is 14 ℃;
(6) heating the enzymolysis material obtained in the step (5) to kill enzyme activity, preferably at 85 ℃, for 45 minutes, then filtering the enzymolysis material by a 300-mesh screen, and keeping filtrate;
(7) decolorizing the filtrate with active carbon, filtering, and vacuum concentrating to 20% of the original volume to obtain concentrated solution;
(8) and (3) sending the concentrated solution to a spray drying device for spray drying to obtain the collagen, wherein the inlet temperature of the spray drying device is preferably 170 ℃, the outlet temperature of the spray drying device is preferably 70 ℃, and the spray pressure is preferably 0.4 MPa.
The fish skin collagen extracted and prepared by the method has the protein content of more than or equal to 96.5 percent, the average molecular weight of 1500-3500 Dalton, suitability for human body absorption, fat content of less than or equal to 0.096 percent, and the increase of the colony number detected after the fish skin collagen is hermetically stored for 12 months in a light-tight environment with the temperature of 23 +/-2 ℃ and the relative humidity of 50 +/-5 percent in-1-3 per mill, and good preservation effect.
Example 2
A method for extracting collagen from fish skin by low-dose irradiation comprises the following steps:
(1) washing fresh fish skin or fish skin leftovers after processing fish meat with water, and crushing to obtain fish skin meat powder;
(2) soaking the minced fish skin meat in a fishy smell removing solution at the temperature of 20 ℃ for 5 hours, washing and drying to prepare dried fish skin powder, wherein the fishy smell removing solution is prepared by the following processes: boiling 12.5 g of astragalus, 10g of cinnamon, 5 g of ginger and 7 g of fennel seed in 450 g of water, filtering, adding 65 g of edible vinegar, 65 g of yellow wine and 14 g of honey into the filtrate, and uniformly stirring;
(3) adding water into the dried fish skin powder and adjusting the pH value of the solution to 6.5 to prepare fish skin feed liquid with the mass concentration of 50%;
(4) transferring the fish skin feed liquid into a transparent glass cup, adding glyceride hydrolase and flavor enzyme, then placing the fish skin feed liquid into a cobalt-60 irradiation field, and carrying out irradiation, degreasing and deodorization treatment to prepare degreased feed liquid, wherein the addition amount of the glyceride hydrolase is 7.5 per mill of the mass of the fish skin feed liquid, the enzyme activity is 1.5 ten thousand U/g, the addition amount of the flavor enzyme is 4.5 per mill of the mass of the fish skin feed liquid, and the enzyme activity is 1.6 ten thousand U/g, and the irradiation method comprises the following steps: the transparent glass cup is 50-60 cm away from the ground surface, 1.2-1.6 m away from a cobalt-60 irradiation source, irradiation is carried out in four sections of time, the irradiation time of each section is 37.5 seconds, the interval is 45 minutes, the irradiation rates are 10Gy/s, 20Gy/s, 30Gy/s and 40Gy/s in sequence, the irradiation unevenness is less than 1.2, and the irradiation environment temperature is kept at 17 ℃;
(5) adding compound decomposition enzyme into the degreasing liquid in the transparent glass cup, continuously irradiating the degreasing liquid to decompose and prepare an enzymolysis material, wherein the adding amount of the compound decomposition enzyme is 6 per mill of the mass of the degreasing liquid, the enzyme activity is 6 ten thousand U/g, the compound decomposition enzyme is preferably composed of trypsin, pepsin and subtilisin, the mass ratio of the trypsin to the pepsin to the subtilisin is 1:1.0:1.75, and the irradiation method comprises the following steps: the transparent glass cup is 55 cm away from the ground surface, 1.4 m away from a cobalt-60 irradiation source, irradiation is carried out in four sections of time, each section of irradiation time is 37.5 seconds, the interval is 45 minutes, the irradiation rates are 6Gy/s, 12Gy/s, 18Gy/s and 24Gy/s in sequence, the irradiation unevenness is less than 1.2, and the irradiation environment temperature is kept at 17 ℃;
(6) heating the enzymolysis material obtained in the step (5) to kill enzyme activity, preferably heating at 90 ℃ for 52.5 minutes, filtering the enzymolysis material by a 350-mesh screen, and keeping filtrate;
(7) decolorizing the filtrate with active carbon, filtering, and vacuum concentrating to 25% of the original volume to obtain concentrated solution;
(8) and (3) sending the concentrated solution to a spray drying device for spray drying to obtain the collagen, wherein the inlet temperature of the spray drying device is 177.5 ℃, the outlet temperature of the spray drying device is 77.5 ℃, and the spray pressure is 0.5 MPa.
The fish skin collagen extracted and prepared by the method has the protein content of more than or equal to 96.5 percent, the average molecular weight of 1500-3500 Dalton, suitability for human body absorption, fat content of less than or equal to 0.096 percent, and the increase of the colony number detected after the fish skin collagen is hermetically stored for 12 months in a light-tight environment with the temperature of 23 +/-2 ℃ and the relative humidity of 50 +/-5 percent in-1-3 per mill, and good preservation effect.
Example 3
A method for extracting collagen from fish skin by low-dose irradiation comprises the following steps:
(1) washing fresh fish skin or fish skin leftovers after processing fish meat with water, and crushing to obtain fish skin meat powder;
(2) soaking the minced fish skin meat in a fishy smell removing solution at the temperature of 30 ℃ for 6 hours, washing and drying to prepare dried fish skin powder, wherein the fishy smell removing solution is prepared by the following processes: boiling 15 g of astragalus membranaceus, 12g of cinnamon, 5.5 g of ginger and 8g of fennel seeds in 500 g of water, filtering, adding 80 g of edible vinegar, 80 g of yellow wine and 16 g of honey into the filtrate, and uniformly stirring;
(3) adding water into the dried fish skin powder and adjusting the pH value of the solution to 7.5 to prepare fish skin feed liquid with mass concentration of 55%;
(4) transferring the fish skin feed liquid into a transparent glass cup, adding glyceride hydrolase and flavor enzyme, then placing the fish skin feed liquid into a cobalt-60 irradiation field, and carrying out irradiation, degreasing and deodorization treatment to prepare degreased feed liquid, wherein the addition amount of the glyceride hydrolase is 9 per mill of the mass of the fish skin feed liquid, the enzyme activity is 2 ten thousand U/g, the addition amount of the flavor enzyme is 6 per mill of the mass of the fish skin feed liquid, and the enzyme activity is 1.2 ten thousand U/g, wherein the irradiation method comprises the following steps: the transparent glass cup is 60cm away from the ground, 1.6m away from a cobalt-60 irradiation source, irradiation is carried out in four sections of time, the irradiation time of each section is 45 seconds, the interval is 45 minutes, the irradiation rates are 10Gy/s, 20Gy/s, 30Gy/s and 40Gy/s in sequence, the irradiation unevenness is less than 1.2, and the irradiation environment temperature is 20 ℃.
(5) Adding compound decomposition enzyme into the degreasing liquid in the transparent glass cup, continuously irradiating the degreasing liquid to decompose and prepare an enzymolysis material, wherein the adding amount of the compound decomposition enzyme is 8 per mill of the mass of the degreasing liquid, the enzyme activity is 5 ten thousand U/g, the compound decomposition enzyme is preferably composed of trypsin, pepsin and subtilisin, the mass ratio of the compound decomposition enzyme to the subtilisin is 1:1.2:2.0, and the irradiation method comprises the following steps: the transparent glass cup is 50-60 cm away from the ground surface, 1.2-1.6 m away from a cobalt-60 irradiation source, irradiation is carried out in four sections of time, the irradiation time of each section is 45 seconds, the interval is 45 minutes, the irradiation rates are 6Gy/s, 12Gy/s, 18Gy/s and 24Gy/s in sequence, the irradiation unevenness is less than 1.2, and the irradiation environment temperature is kept at 20 ℃;
(6) heating the enzymolysis material obtained in the step (5) to kill enzyme activity, preferably at 95 ℃, for 60 minutes, then filtering the enzymolysis material by a 400-mesh screen, and keeping filtrate;
(7) decoloring the filtrate by active carbon, then filtering, and carrying out vacuum concentration to 20-30% of the original volume to prepare a concentrated solution;
(8) and (3) sending the concentrated solution to a spray drying device for spray drying to obtain the collagen, wherein the inlet temperature of the spray drying device is 185 ℃, the outlet temperature of the spray drying device is 85 ℃, and the spray pressure is 0.6 MPa.
The fish skin collagen extracted and prepared by the method has the protein content of more than or equal to 96.5 percent, the average molecular weight of 1500-3500 Dalton, suitability for human body absorption, the fat content of less than or equal to 0.096 percent, the increase of the colony number detected after the fish skin collagen is hermetically stored for 12 months in a light-proof environment with the temperature of 23 +/-2 ℃ and the relative humidity of 50 +/-5 percent is-1-3 per mill, and the fresh-keeping effect is good.
Claims (2)
1. A method for extracting collagen from fish skin by low-dose irradiation comprises the following steps:
(1) washing fresh fish skin or fish skin leftovers after processing fish meat with water, and crushing to obtain fish skin meat powder;
(2) soaking the fish skin minced meat in a deodorization solution at the temperature of 10-30 ℃ for 4-6 hours, washing and drying to prepare fish skin dry powder;
(3) adding water into the dried fish skin powder and adjusting the pH value to 5.5-7.5 to prepare fish skin feed liquid with the mass concentration of 45-55%;
(4) transferring the fish skin feed liquid into a transparent glass cup, adding glyceride hydrolase and flavor enzyme, and then placing in a cobalt-60 irradiation field to prepare degreased feed liquid through irradiation, degreasing and deodorization treatment;
(5) adding compound decomposition enzyme into the degreased feed liquid in the transparent glass cup, and continuously irradiating the degreased feed liquid to decompose to prepare an enzymolysis material;
(6) heating the enzymolysis material at 85-95 ℃ for 45-60 minutes, filtering by using a 300-400 mesh screen, and keeping filtrate;
(7) decoloring the filtrate by active carbon, then filtering, and carrying out vacuum concentration to 20-30% of the original volume to prepare a concentrated solution;
(8) sending the concentrated solution to spray drying equipment for spray drying to obtain collagen;
the fishy smell removing solution in the step (2) is prepared by the following processes: placing 10-15 parts by weight of astragalus membranaceus, 8-12 parts by weight of cinnamon, 4.5-5.5 parts by weight of ginger and 6-8 parts by weight of fennel seeds in 400-500 parts by weight of water for boiling, then filtering, adding 50-80 parts by weight of edible vinegar, 50-80 parts by weight of yellow wine and 12-16 parts by weight of honey into filtrate, and uniformly stirring to prepare a fishy smell removing solution;
in the step (4), the addition amount of the glyceride hydrolase is 6-9 per thousand of the mass of the fish skin feed liquid, the enzyme activity is 2-3 ten thousand U/g, the addition amount of the flavor enzyme is 3-6 per thousand of the mass of the fish skin feed liquid, and the enzyme activity is 1.2-1.8 ten thousand U/g;
the compound lyase in the step (5) consists of trypsin, pepsin and subtilisin, and the mass ratio of the trypsin to the pepsin to the subtilisin is 1: 0.8-1.2: 1.5-2.0; the adding amount of the compound decomposition enzyme is 4-8 per mill of the mass of the degreasing feed liquid, and the enzyme activity is 5-7 ten thousand U/g; irradiating for four periods of time in the step (4), wherein the irradiation time of each period of time is 30-45 seconds, the interval is 45 minutes, and the irradiation rates are 10Gy/s, 20Gy/s, 30Gy/s and 40Gy/s in sequence;
irradiating for four periods of time in the step (5), wherein the irradiation time of each period of time is 30-45 seconds, the interval is 45 minutes, and the irradiation rates are 6Gy/s, 12Gy/s, 18Gy/s and 24Gy/s in sequence;
the irradiation method in the step (4) and the step (5) comprises the following steps: the transparent glass cup is 50-60 cm away from the ground surface, 1.2-1.6 m away from a cobalt-60 irradiation source, the irradiation unevenness is less than 1.2, and the irradiation environment temperature is 14-20 ℃.
2. The method for extracting collagen from fish skin by low-dose irradiation as claimed in claim 1, wherein the inlet temperature of the spray drying equipment is 170-185 ℃, the outlet temperature is 70-85 ℃, and the spraying pressure is 0.4-0.6 MPa.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103773830A (en) * | 2014-01-21 | 2014-05-07 | 曾佳 | Method for extracting collagen from fish scales |
| CN103789381A (en) * | 2014-02-14 | 2014-05-14 | 钟春燕 | Method for rapidly extracting fish collagen |
| CN103829030A (en) * | 2014-03-27 | 2014-06-04 | 海南华研生物科技有限公司 | Method for producing low-fish-smell fish skin collagen |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103773830A (en) * | 2014-01-21 | 2014-05-07 | 曾佳 | Method for extracting collagen from fish scales |
| CN103789381A (en) * | 2014-02-14 | 2014-05-14 | 钟春燕 | Method for rapidly extracting fish collagen |
| CN103829030A (en) * | 2014-03-27 | 2014-06-04 | 海南华研生物科技有限公司 | Method for producing low-fish-smell fish skin collagen |
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