CN107012096A - A kind of ferment bacterium fermentation process - Google Patents
A kind of ferment bacterium fermentation process Download PDFInfo
- Publication number
- CN107012096A CN107012096A CN201710285960.0A CN201710285960A CN107012096A CN 107012096 A CN107012096 A CN 107012096A CN 201710285960 A CN201710285960 A CN 201710285960A CN 107012096 A CN107012096 A CN 107012096A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- ferment bacterium
- pineapple
- fermentation process
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
Abstract
The invention discloses a kind of ferment bacterium fermentation process, including culture medium, inoculation, training orientation and matrix treatments are prepared, the ferment bacterium opportunistic pathogen of import is cultivated by the method for science so that the quantity increase of ferment bacterium, greatly reduce the cost of ferment bacterium.
Description
Technical field
The present invention relates to technical field of bioengineering, more particularly to a kind of ferment bacterium fermentation process.
Background technology
The word of ferment bacterium one is a kind of call of the Japanese to enzyme (enzyme), refers to the macromolecular complex with biocatalytic Activity
The zymotic fluid with bioactive substance or fermented product that are obtained through microbial fermentation, were referred to as ferment by matter later.In view of
Some producers only focus on fruit, and particularly with the addition of different types of fruit, the effect of microorganism in fermentation process is not noted,
Ignore the application to some functional materials, it is necessary to which application tradition and science collocation make ferment product, expand and ensure
Ferment function, is that common people's health provides safeguard.Ferment product development and application are also vulnerable to the influence of living contaminants, therefore
It is necessary the fermentation technique of applied science to ensure product quality.
At present, the ferment bacterium that we use is that every matrix is containing 2 kilograms of ferment bacteriums, cartridge clip 65- from Japanese import mostly
70 yuan, for production enterprise, the use cost of ferment bacterium is very high, is unfavorable for enterprise development.
The content of the invention
A kind of ferment bacterium fermentation process proposed by the present invention, reduces the cost of ferment bacterium, reduces the cost of enterprise.
In order to solve the above-mentioned technical problem, the present invention is using the basic ideas of technical scheme：A kind of ferment bacterium fermentation side
Method, comprises the following steps：
1) culture medium, is prepared：Dregs of beans, rice bran, brown sugar and pineapple are taken respectively, and its composition by weight proportioning is：Dregs of beans 20-30
Part, 40-50 part of rice bran, 2-4 part of brown sugar, 5-9 part of pineapple, peeling is cut into slices after pineapple is clean, then by dregs of beans, rice bran, brown sugar and
Uniformly mixing is put into autoclave to pineapple, is heated to 130-140 DEG C, continues 30 minutes, carries out high-temperature sterilization, culture medium is made；
2), it is inoculated with：Culture medium is put into fermentation tank, while adding 60%-65% water, fermentation tank is then put into nothing
Bacterium room, then adds ferment bacterium opportunistic pathogen in culture medium, and stir；
3), training orientation：
A, period 1：Fermentation calefaction, by the temperature control in fermentation tank at 40-45 DEG C, is kept for 3 days；
B, second round：The water tariff collection of culture medium is in 48%-52%, and temperature control is kept for 4 days at 50-55 DEG C；
C, period 3：The water tariff collection of culture medium is in 40%-45%, and temperature control is kept for 5 days at 55-60 DEG C；
4), the culture medium after culture is spread out, dried, ferment bacterium is obtained.
Further, the water added in the step 2 and 3 is cold boiling water.
Further, high-temp sterilizing is passed through before the instrument use stirred in the step 2.
Beneficial effects of the present invention are：A kind of ferment bacterium fermentation process of the present invention, by the method for science to import
Ferment bacterium opportunistic pathogen is cultivated so that the quantity increase of ferment bacterium, greatly reduces the cost of ferment bacterium.
Embodiment
The embodiment to the present invention is described further below.Herein it should be noted that for these implementations
The explanation of mode is used to help understand the present invention, but does not constitute limitation of the invention.In addition, invention described below
As long as involved technical characteristic does not constitute conflict and can be just mutually combined each other in each embodiment.
A kind of ferment bacterium fermentation process, comprises the following steps：
1) culture medium, is prepared：Dregs of beans, rice bran, brown sugar and pineapple are taken respectively, and its composition by weight proportioning is：Dregs of beans 20-30
Part, 40-50 part of rice bran, 2-4 part of brown sugar, 5-9 part of pineapple, peeling is cut into slices after pineapple is clean, then by dregs of beans, rice bran, brown sugar and
Uniformly mixing is put into autoclave to pineapple, is heated to 130-140 DEG C, continues 30 minutes, carries out high-temperature sterilization, culture medium is made；
2), it is inoculated with：Culture medium is put into fermentation tank, while adding 60%-65% water, fermentation tank is then put into nothing
Bacterium room, then adds ferment bacterium opportunistic pathogen in culture medium, and stir；
3), training orientation：
A, period 1：Fermentation calefaction, by the temperature control in fermentation tank at 40-45 DEG C, is kept for 3 days；
B, second round：The water tariff collection of culture medium is in 48%-52%, and temperature control is kept for 4 days at 50-55 DEG C；
C, period 3：The water tariff collection of culture medium is in 40%-45%, and temperature control is kept for 5 days at 55-60 DEG C；
4), the culture medium after culture is spread out, dried, ferment bacterium is obtained.
The water added in step 2 and 3 is cold boiling water.
To pass through high-temp sterilizing before the instrument use stirred in step 2.
By the method for science, the ferment bacterium opportunistic pathogen to import is cultivated so that containing 1 kilogram of ferment in every matrix
Bacterium, and cost is reduced to 10 yuan, enters after cultivation, every gram of microorganism viable bacteria number is more than or equal to 0.5 hundred million.
Embodiments of the present invention are explained in detail above, but the invention is not restricted to described embodiment.It is right
For those skilled in the art, in the case where not departing from the principle of the invention and spirit, these embodiments are carried out many
Change, modification, replacement and modification are planted, are still fallen within protection scope of the present invention.
Claims (3)
1. a kind of ferment bacterium fermentation process, it is characterised in that：Comprise the following steps：
1) culture medium, is prepared：Dregs of beans, rice bran, brown sugar and pineapple are taken respectively, and its composition by weight proportioning is：20-30 parts of dregs of beans, rice
40-50 parts of chaff, 2-4 parts of brown sugar, 5-9 parts of pineapple, pineapple removes the peel section after cleaning, then that dregs of beans, rice bran, brown sugar and pineapple is equal
Even mixing is put into autoclave, is heated to 130-140 DEG C, continues 30 minutes, carries out high-temperature sterilization, culture medium is made；
2), it is inoculated with：Culture medium is put into fermentation tank, while adding 60%-65% water, is then put into fermentation tank sterile
Room, then adds ferment bacterium opportunistic pathogen in culture medium, and stir；
3), training orientation：
A, period 1：Fermentation calefaction, by the temperature control in fermentation tank at 40-45 DEG C, is kept for 3 days；
B, second round：The water tariff collection of culture medium is in 48%-52%, and temperature control is kept for 4 days at 50-55 DEG C；
C, period 3：The water tariff collection of culture medium is in 40%-45%, and temperature control is kept for 5 days at 55-60 DEG C；
4), matrix treatments：Culture medium after culture is spread out, dried, ferment bacterium is obtained.
2. a kind of ferment bacterium fermentation process according to claim 1, it is characterised in that：In the step 2 and 3 plus water be
Cold boiling water.
3. a kind of ferment bacterium fermentation process according to claim 1, it is characterised in that：The instrument stirred in the step 2
To pass through high-temp sterilizing before use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710285960.0A CN107012096A (en) | 2017-04-27 | 2017-04-27 | A kind of ferment bacterium fermentation process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710285960.0A CN107012096A (en) | 2017-04-27 | 2017-04-27 | A kind of ferment bacterium fermentation process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107012096A true CN107012096A (en) | 2017-08-04 |
Family
ID=59448336
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710285960.0A Pending CN107012096A (en) | 2017-04-27 | 2017-04-27 | A kind of ferment bacterium fermentation process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107012096A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103844266A (en) * | 2014-03-25 | 2014-06-11 | 容瑜 | Natural conditioning and nourishing ferment and manufacturing method thereof |
| CN105146402A (en) * | 2015-09-09 | 2015-12-16 | 安徽管仲宫神生物科技有限公司 | Preparation method of purple sweet potato enzyme powder |
| CN105146660A (en) * | 2015-09-09 | 2015-12-16 | 安徽管仲宫神生物科技有限公司 | Natural fruit and vegetable enzyme beverage and preparation method thereof |
| KR20160070508A (en) * | 2014-12-10 | 2016-06-20 | (주)하이모 | Fermented Food for Preventing Hair Loss or Promoting Hair Growth |
-
2017
- 2017-04-27 CN CN201710285960.0A patent/CN107012096A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103844266A (en) * | 2014-03-25 | 2014-06-11 | 容瑜 | Natural conditioning and nourishing ferment and manufacturing method thereof |
| KR20160070508A (en) * | 2014-12-10 | 2016-06-20 | (주)하이모 | Fermented Food for Preventing Hair Loss or Promoting Hair Growth |
| CN105146402A (en) * | 2015-09-09 | 2015-12-16 | 安徽管仲宫神生物科技有限公司 | Preparation method of purple sweet potato enzyme powder |
| CN105146660A (en) * | 2015-09-09 | 2015-12-16 | 安徽管仲宫神生物科技有限公司 | Natural fruit and vegetable enzyme beverage and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刘加友等: ""微生物酵素食品研究进展"", 《食品与发酵工业》 * |
| 江晃荣: "《酵素第1版》", 30 September 2014, 吉林科学技术出版社 * |
| 陈庶来等: ""糙米酵素发酵工艺的研究"", 《食品科学》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103749796B (en) | Manufacturing method of green tea fermented beverage | |
| Mensah et al. | Use of pineapple waste for single cell protein (SCP) production and the effect of substrate concentration on the yield | |
| CN1994116A (en) | Pure breed fermentation process for producing tasteless preserved soybean | |
| CN103289937A (en) | Method for producing bacillus laterosporus live bacteria by high density solid fermentation | |
| CN107586725B (en) | Cordyceps liquid culture medium and method for culturing cordyceps by using same | |
| CN101717746B (en) | Method for producing spores of clostridium butyricum and application thereof in livestock and poultry rearing | |
| CN105876782A (en) | Onion enzymes and preparation method thereof | |
| CN101153259A (en) | Disinfection technique of zero-PU value | |
| CN103087962B (en) | Method for high-density culture of lactobacillus for biological acidulated rice steeping | |
| KR100726842B1 (en) | Liquid fertilizer prepared by using waste water generated from the process of blood powder production, method of preparation thereof and crops cultivated by using the same | |
| CN107012096A (en) | A kind of ferment bacterium fermentation process | |
| CN106213096A (en) | A kind of method that enzyme beverage prepared by Fructus Musae that ferments | |
| CN103525682B (en) | Vinegar brewing method preventing mycoderm and vinegar flies from being generated | |
| KR20160115965A (en) | Production method for reduced-carbohydrate health food product | |
| CN105639542A (en) | Chili base and preparation method thereof | |
| CN106616973A (en) | Preparation method of acer truncatum pterodium living bacteria enzymes stock solution | |
| CN109937975B (en) | Standardized earthworm breeding method | |
| CN106666076A (en) | Strain composition suitable for fermenting needle mushroom residues and brewer's grains, and application thereof | |
| CN111363692A (en) | Complex microbial inoculant and application of fermentation product thereof | |
| CN112772807A (en) | Preparation method of eucommia ulmoides fermented beverage | |
| CN104126837B (en) | A kind of preparation method of germinating unpolished rice beverage | |
| CN109567179B (en) | Natural fruit enzyme and preparation process thereof | |
| CN115429826B (en) | Antibacterial growth-promoting fermented traditional Chinese medicine preparation and preparation method thereof | |
| CN107794204A (en) | A kind of processing method of selenium-rich pear fruit vinegar | |
| CN106591187A (en) | Lactobacillus culture medium for production of animal feed and preparation and application of lactobacillus culture medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170804 |
|
| RJ01 | Rejection of invention patent application after publication |