CN115429826B - Antibacterial growth-promoting fermented traditional Chinese medicine preparation and preparation method thereof - Google Patents
Antibacterial growth-promoting fermented traditional Chinese medicine preparation and preparation method thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/49—Fagaceae (Beech family), e.g. oak or chestnut
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C12R2001/25—Lactobacillus plantarum
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
Abstract
The invention discloses a fermentation traditional Chinese medicine preparation for inhibiting bacteria and promoting growth and a preparation method thereof, wherein the fermentation traditional Chinese medicine preparation for inhibiting bacteria and promoting growth is prepared by fermenting a traditional Chinese medicine composition and probiotics; the traditional Chinese medicine composition comprises gallnut, pericarpium Granati and oak bowl; the probiotics comprise bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae. The traditional Chinese medicine preparation is rich in tannic acid, has the effects of inhibiting bacteria, astringing, resisting diarrhea and promoting growth, and has the advantages of increased palatability, increased feed intake and increased feed conversion rate after fermentation. The fermented traditional Chinese medicine preparation has an inhibiting effect on common intestinal pathogenic bacteria, and can promote the growth of broiler chickens.
Description
Technical Field
The invention relates to the technical field of livestock breeding, in particular to a fermented traditional Chinese medicine preparation for inhibiting bacteria and promoting growth and a preparation method thereof.
Background
At present, epidemic disease frequency and excessive use of antibiotics in the broiler breeding industry are one of the most serious challenges facing sustainable development of the broiler breeding industry in China. Disabling or reducing feed antibiotics has become an international social consensus. Under the background of comprehensive resistance limiting and resistance forbidding, the tie-off product becomes an urgent need for healthy development of the broiler raising industry. The probiotics and the traditional Chinese medicine preparation have the advantages of nature, safety, no residue, no pollution and the like, so that if the probiotics and the traditional Chinese medicine preparation can be adopted to develop the fermented traditional Chinese medicine preparation with antibacterial and growth promoting functions, the preparation has wide market prospect in the broiler chicken breeding industry.
Disclosure of Invention
The invention provides a fermented traditional Chinese medicine preparation for inhibiting bacteria and promoting growth and a preparation method thereof.
The technical scheme of the invention is as follows:
the antibacterial growth-promoting fermented traditional Chinese medicine preparation is prepared from a traditional Chinese medicine composition and probiotics by fermentation;
the traditional Chinese medicine composition comprises gallnut, pericarpium Granati and oak bowl;
the probiotics comprise bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae.
Preferably, the traditional Chinese medicine composition comprises the following components in parts by weight: 20-50 parts of gallnut, 20-30 parts of pomegranate rind and 10-20 parts of acorn bowl.
The preparation method of the antibacterial growth-promoting fermented traditional Chinese medicine preparation comprises the following steps:
(1) Inoculating the bacillus subtilis, the lactobacillus plantarum, the enterococcus faecium and the saccharomyces cerevisiae into a sterilized independent culture medium for culture, and respectively preparing a bacillus subtilis activating solution, a lactobacillus plantarum activating solution, an enterococcus faecium activating solution and a saccharomyces cerevisiae activating solution after heat preservation and activation;
(2) Weighing the components of the traditional Chinese medicine composition according to parts by weight, respectively crushing, uniformly mixing the components of the traditional Chinese medicine composition to obtain a first mixture, adding water into the first mixture, uniformly mixing to obtain a second mixture, introducing steam into the second mixture for steam heating treatment, and cooling to obtain the traditional Chinese medicine mixture;
(3) Uniformly mixing the traditional Chinese medicine mixture with fermentation auxiliary materials according to the proportion of 1 (0.2-0.3), and respectively inoculating bacillus subtilis activating solution, lactobacillus plantarum activating solution, enterococcus faecium activating solution and saccharomyces cerevisiae activating solution, and fermenting to obtain the antibacterial growth-promoting fermentation traditional Chinese medicine preparation.
Preferably, in the step (3), the fermentation auxiliary material is any one of corn flour, bran or soybean meal.
Preferably, in the step (1), the bacillus subtilis, the lactobacillus plantarum, the enterococcus faecium and the saccharomyces cerevisiae are respectively inoculated into a sterilized independent culture medium according to the inoculation amount of 1-2 percent for culture, and after heat preservation and activation for 2 hours, the bacillus subtilis activating solution, the lactobacillus plantarum activating solution, the enterococcus faecium activating solution and the saccharomyces cerevisiae activating solution are respectively prepared.
Preferably, in step (2), 15-30 parts by weight of water is added to the first mixture.
Preferably, in the step (2), the components of the traditional Chinese medicine composition are respectively weighed according to parts by weight and respectively crushed to 60-80 meshes.
Preferably, in the step (2), parameters of steam heating treatment by introducing steam into the second mixture are as follows: raising the temperature of the materials to 80-85 ℃ and treating for 2h.
Preferably, in the step (3), the parameters of the fermentation treatment are: fermenting at 37deg.C for 3-5 days.
Preferably, in the step (3), the addition amounts of the bacillus subtilis activating solution, the lactobacillus plantarum activating solution, the enterococcus faecium activating solution and the saccharomyces cerevisiae activating solution are respectively (0.1-0.5)%, (0.1-0.5)%, and (0.1-0.5)% of the weight of the traditional Chinese medicine mixture.
The invention has the beneficial effects that:
1. according to the invention, 4 probiotics including bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae are selected for fermentation, and the bacillus and the saccharomycetes produce enzymes, so that the precipitation of tannic acid in the traditional Chinese medicine composition can be promoted; lactobacillus plantarum and enterococcus faecium are fermented by using the traditional Chinese medicine mixture and fermentation auxiliary materials to produce antibacterial substances such as lactic acid and the like.
2. The fermented traditional Chinese medicine preparation has an inhibiting effect on common intestinal pathogenic bacteria, and particularly has an inhibiting effect on staphylococcus aureus.
3. The fermented traditional Chinese medicine preparation can promote the growth of broiler chickens and improve the feed conversion rate.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in further detail below.
Example 1
The antibacterial growth-promoting fermented traditional Chinese medicine preparation is prepared from a traditional Chinese medicine composition and probiotics by fermentation;
the traditional Chinese medicine composition comprises gallnut, pericarpium Granati and oak bowl;
the probiotics comprise bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae.
The traditional Chinese medicine composition comprises the following components in parts by weight: 20 parts of Chinese gall, 30 parts of pomegranate rind and 17 parts of oak bowl.
The preparation method of the antibacterial growth-promoting fermented traditional Chinese medicine preparation comprises the following steps:
(1) Inoculating the bacillus subtilis, the lactobacillus plantarum, the enterococcus faecium and the saccharomyces cerevisiae into a sterilized independent culture medium for culture, and respectively preparing a bacillus subtilis activating solution, a lactobacillus plantarum activating solution, an enterococcus faecium activating solution and a saccharomyces cerevisiae activating solution after heat preservation and activation;
(2) Weighing the components of the traditional Chinese medicine composition according to parts by weight, respectively crushing, uniformly mixing the components of the traditional Chinese medicine composition to obtain a first mixture, adding water into the first mixture, uniformly mixing to obtain a second mixture, introducing steam into the second mixture for steam heating treatment, and cooling to obtain the traditional Chinese medicine mixture;
(3) And uniformly mixing the traditional Chinese medicine mixture with fermentation auxiliary materials according to a ratio of 1:0.2, respectively inoculating bacillus subtilis activating solution, lactobacillus plantarum activating solution, enterococcus faecium activating solution and saccharomyces cerevisiae activating solution, and fermenting to obtain the antibacterial growth-promoting fermented traditional Chinese medicine preparation.
In the step (3), the fermentation auxiliary material is corn flour.
In the step (1), bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae are respectively inoculated into a sterilized independent culture medium according to the inoculation amount of 1.5 percent for culture, and after heat preservation and activation for 2 hours, bacillus subtilis activating solution, lactobacillus plantarum activating solution, enterococcus faecium activating solution and saccharomyces cerevisiae activating solution are respectively prepared.
In step (2), 24 parts by weight of water is added to the first mixture.
In the step (2), the components of the traditional Chinese medicine composition are respectively weighed according to the weight parts and are respectively crushed to 70 meshes.
In the step (2), the parameters of the steam heating treatment performed by introducing steam into the second mixture are as follows: the temperature of the material was raised to 85℃and treated for 2h.
In the step (3), parameters of fermentation treatment are as follows: the ambient temperature is 37 ℃, and the fermentation is carried out for 5 days.
In the step (3), the addition amounts of the bacillus subtilis activating solution, the lactobacillus plantarum activating solution, the enterococcus faecium activating solution and the saccharomyces cerevisiae activating solution are respectively 0.3%, 0.5%, 0.1% and 0.3% of the weight of the traditional Chinese medicine mixture.
Example 2
The antibacterial growth-promoting fermented traditional Chinese medicine preparation is prepared from a traditional Chinese medicine composition and probiotics by fermentation;
the traditional Chinese medicine composition comprises gallnut, pericarpium Granati and oak bowl;
the probiotics comprise bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae.
The traditional Chinese medicine composition comprises the following components in parts by weight: 42 parts of gallnut, 28 parts of pomegranate rind and 11 parts of acorn bowl.
The preparation method of the antibacterial growth-promoting fermented traditional Chinese medicine preparation comprises the following steps:
(1) Inoculating the bacillus subtilis, the lactobacillus plantarum, the enterococcus faecium and the saccharomyces cerevisiae into a sterilized independent culture medium for culture, and respectively preparing a bacillus subtilis activating solution, a lactobacillus plantarum activating solution, an enterococcus faecium activating solution and a saccharomyces cerevisiae activating solution after heat preservation and activation;
(2) Weighing the components of the traditional Chinese medicine composition according to parts by weight, respectively crushing, uniformly mixing the components of the traditional Chinese medicine composition to obtain a first mixture, adding water into the first mixture, uniformly mixing to obtain a second mixture, introducing steam into the second mixture for steam heating treatment, and cooling to obtain the traditional Chinese medicine mixture;
(3) And uniformly mixing the traditional Chinese medicine mixture with fermentation auxiliary materials according to a ratio of 1:0.23, respectively inoculating bacillus subtilis activating solution, lactobacillus plantarum activating solution, enterococcus faecium activating solution and saccharomyces cerevisiae activating solution, and fermenting to obtain the antibacterial growth-promoting fermented traditional Chinese medicine preparation.
In the step (3), the fermentation auxiliary material is bran.
In the step (1), bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae are respectively inoculated into a sterilized independent culture medium according to the inoculation amount of 1 percent for culture, and after heat preservation and activation for 2 hours, bacillus subtilis activating solution, lactobacillus plantarum activating solution, enterococcus faecium activating solution and saccharomyces cerevisiae activating solution are respectively prepared.
In step (2), 30 parts by weight of water is added to the first mixture.
In the step (2), the components of the traditional Chinese medicine composition are respectively weighed according to the weight parts and are respectively crushed to 60 meshes.
In the step (2), the parameters of the steam heating treatment performed by introducing steam into the second mixture are as follows: the temperature of the material was raised to 82℃and treated for 2h.
In the step (3), parameters of fermentation treatment are as follows: the ambient temperature is 37 ℃, and the fermentation is carried out for 4 days.
In the step (3), the addition amounts of the bacillus subtilis activating solution, the lactobacillus plantarum activating solution, the enterococcus faecium activating solution and the saccharomyces cerevisiae activating solution are respectively 0.1%, 0.2%, 0.4% and 0.2% of the weight of the traditional Chinese medicine mixture.
Example 3
The antibacterial growth-promoting fermented traditional Chinese medicine preparation is prepared from a traditional Chinese medicine composition and probiotics by fermentation;
the traditional Chinese medicine composition comprises gallnut, pericarpium Granati and oak bowl;
the probiotics comprise bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae.
The traditional Chinese medicine composition comprises the following components in parts by weight: 50 parts of Chinese gall, 20 parts of pomegranate rind and 10 parts of acorn bowl.
The preparation method of the antibacterial growth-promoting fermented traditional Chinese medicine preparation comprises the following steps:
(1) Inoculating the bacillus subtilis, the lactobacillus plantarum, the enterococcus faecium and the saccharomyces cerevisiae into a sterilized independent culture medium for culture, and respectively preparing a bacillus subtilis activating solution, a lactobacillus plantarum activating solution, an enterococcus faecium activating solution and a saccharomyces cerevisiae activating solution after heat preservation and activation;
(2) Weighing the components of the traditional Chinese medicine composition according to parts by weight, respectively crushing, uniformly mixing the components of the traditional Chinese medicine composition to obtain a first mixture, adding water into the first mixture, uniformly mixing to obtain a second mixture, introducing steam into the second mixture for steam heating treatment, and cooling to obtain the traditional Chinese medicine mixture;
(3) And uniformly mixing the traditional Chinese medicine mixture with fermentation auxiliary materials according to a ratio of 1:0.3, respectively inoculating bacillus subtilis activating solution, lactobacillus plantarum activating solution, enterococcus faecium activating solution and saccharomyces cerevisiae activating solution, and fermenting to obtain the antibacterial growth-promoting fermented traditional Chinese medicine preparation.
In the step (3), the fermentation auxiliary material is soybean meal.
In the step (1), bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae are respectively inoculated into a sterilized independent culture medium according to the inoculation amount of 2 percent for culture, and after heat preservation and activation for 2 hours, bacillus subtilis activating solution, lactobacillus plantarum activating solution, enterococcus faecium activating solution and saccharomyces cerevisiae activating solution are respectively prepared.
In step (2), 16 parts by weight of water is added to the first mixture.
In the step (2), the components of the traditional Chinese medicine composition are respectively weighed according to the weight parts and respectively crushed to 80 meshes.
In the step (2), the parameters of the steam heating treatment performed by introducing steam into the second mixture are as follows: the temperature of the material was raised to 80℃and treated for 2h.
In the step (3), parameters of fermentation treatment are as follows: the ambient temperature is 37 ℃, and the fermentation is carried out for 4 days.
In the step (3), the addition amounts of the bacillus subtilis activating solution, the lactobacillus plantarum activating solution, the enterococcus faecium activating solution and the saccharomyces cerevisiae activating solution are respectively 0.5%, 0.4%, 0.5% and 0.1% of the weight of the traditional Chinese medicine mixture.
Example 4
The antibacterial growth-promoting fermented traditional Chinese medicine preparation is prepared from a traditional Chinese medicine composition and probiotics by fermentation;
the traditional Chinese medicine composition comprises gallnut, pericarpium Granati and oak bowl;
the probiotics comprise bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae.
The traditional Chinese medicine composition comprises the following components in parts by weight: 37 parts of nutgall, 22 parts of pericarpium Granati and 20 parts of oak bowl.
The preparation method of the antibacterial growth-promoting fermented traditional Chinese medicine preparation comprises the following steps:
(1) Inoculating the bacillus subtilis, the lactobacillus plantarum, the enterococcus faecium and the saccharomyces cerevisiae into a sterilized independent culture medium for culture, and respectively preparing a bacillus subtilis activating solution, a lactobacillus plantarum activating solution, an enterococcus faecium activating solution and a saccharomyces cerevisiae activating solution after heat preservation and activation;
(2) Weighing the components of the traditional Chinese medicine composition according to parts by weight, respectively crushing, uniformly mixing the components of the traditional Chinese medicine composition to obtain a first mixture, adding water into the first mixture, uniformly mixing to obtain a second mixture, introducing steam into the second mixture for steam heating treatment, and cooling to obtain the traditional Chinese medicine mixture;
(3) And uniformly mixing the traditional Chinese medicine mixture with fermentation auxiliary materials according to a ratio of 1:0.27, respectively inoculating bacillus subtilis activating solution, lactobacillus plantarum activating solution, enterococcus faecium activating solution and saccharomyces cerevisiae activating solution, and fermenting to obtain the antibacterial growth-promoting fermented traditional Chinese medicine preparation.
In the step (3), the fermentation auxiliary material is bran.
In the step (1), bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae are respectively inoculated into a sterilized independent culture medium according to the inoculation amount of 1.8 percent for culture, and after heat preservation and activation for 2 hours, bacillus subtilis activating solution, lactobacillus plantarum activating solution, enterococcus faecium activating solution and saccharomyces cerevisiae activating solution are respectively prepared.
In step (2), 15 parts by weight of water is added to the first mixture.
In the step (2), the components of the traditional Chinese medicine composition are respectively weighed according to the weight parts and respectively crushed to 80 meshes.
In the step (2), the parameters of the steam heating treatment performed by introducing steam into the second mixture are as follows: the temperature of the material was raised to 83℃and treated for 2h.
In the step (3), parameters of fermentation treatment are as follows: the ambient temperature is 37 ℃, and the fermentation is carried out for 3 days.
In the step (3), the addition amounts of the bacillus subtilis activating solution, the lactobacillus plantarum activating solution, the enterococcus faecium activating solution and the saccharomyces cerevisiae activating solution are respectively 0.4%, 0.1%, 0.2% and 0.5% of the weight of the traditional Chinese medicine mixture.
The antibacterial and growth-promoting fermented traditional Chinese medicine preparation prepared in the examples 1-4 is subjected to antibacterial and animal tests, and the test treatment is specifically as follows:
1. antibacterial test of antibacterial growth-promoting fermented traditional Chinese medicine preparation
Coli @E.coli) Streaking 6 intestinal pathogenic bacteria plates of K88, K99, O139, O157 and staphylococcus aureus and salmonella, and culturing at 37 ℃ for 20 hours; single colonies were picked up in MH liquid medium and shake cultured at 37 ℃ for 16h, after the culture was completed, the number of indicator bacteria was adjusted to od600=0.5.
5g of the antibacterial growth-promoting fermented traditional Chinese medicine preparation prepared in the examples 1-4 are respectively weighed, sterile distilled water and 60% ethanol are respectively added into the two parts by weight according to the weight ratio of 1:5, the mixture is vortexed for 5min, the mixture is vortexed again after being subjected to ultrasonic treatment for 20min, the pH is adjusted to be neutral, and the mixture is centrifuged, so that supernatant fluid is reserved.
Taking MH solid culture medium, sterilizing at 121 ℃ for 20 minutes, cooling to 42 ℃, adding indicator bacteria liquid respectively according to the ratio of bacteria liquid to culture medium=500 mu L to 100 mL, and uniformly mixing. The bacteria-containing culture medium is poured into the flat plate, so that the thickness of the culture medium reaches more than 4 mm. After the culture medium is solidified, uniformly punching holes by using a puncher, adding 200 mu L of fermented traditional Chinese medicine preparation extracting solution into each hole, standing at 4 ℃ for 6 hours, and culturing at 37 ℃ for 16 hours to observe a bacteriostasis result. The antibacterial results of the water extraction method and the alcohol extraction method show that the antibacterial growth-promoting fermented traditional Chinese medicine preparation prepared by the invention has the best antibacterial effect on staphylococcus aureus in all indicator bacteria. The water extraction method of the antibacterial growth-promoting fermentation traditional Chinese medicine preparation prepared by the invention has the best antibacterial effect on staphylococcus aureus; compared with the water extraction method, the alcohol extraction method has better antibacterial effect on escherichia coli and salmonella.
TABLE 1 diameter of antibacterial zone of antibacterial growth-promoting fermented Chinese medicinal preparation prepared by the invention (water extraction method)
TABLE 2 diameter of antibacterial zone of antibacterial growth-promoting fermented Chinese medicinal preparation prepared by the invention (alcohol extraction method)
2. Influence of antibacterial growth-promoting fermented traditional Chinese medicine preparation on 817 broiler chicken growth performance
150 broilers are randomly selected 817 and randomly divided into 5 groups according to the principles of male and female halves, basic ration is fed to a control group, and four test groups (respectively, example 1, example 2, example 3 and example 4) are fed with basic ration plus 1kg/T of the antibacterial growth-promoting fermented traditional Chinese medicine preparation prepared by the invention, specifically:
test group (example 1): feeding basic ration plus 1kg/T of the antibacterial growth-promoting fermented traditional Chinese medicine preparation prepared in the example 1;
test group (example 2): feeding basic ration plus 1kg/T of the antibacterial growth-promoting fermented traditional Chinese medicine preparation prepared in the example 1;
test group (example 3): feeding basic ration plus 1kg/T of the antibacterial growth-promoting fermented traditional Chinese medicine preparation prepared in the example 1;
test group (example 4): feeding basic ration plus 1kg/T of the antibacterial growth-promoting fermented traditional Chinese medicine preparation prepared in the example 1;
the feeding test stage is 1-35d.
The test results show that none of the four groups of test groups (example 1, example 2, example 3, example 4) died; the average daily gain of the four test groups (example 1, example 2, example 3, example 4) was increased and the feed conversion ratio was decreased compared to the control group. The results show that the antibacterial growth-promoting fermented traditional Chinese medicine preparation prepared by the invention can reduce the death rate of broiler chickens and promote the growth of the broiler chickens.
TABLE 3 influence of fermented Chinese medicinal preparation on 817 broiler growth performance
| Project | Control group | Example 1 | Example 2 | Example 3 | Example 4 |
| Initial quantity (only) | 30 | 30 | 30 | 30 | 30 |
| End number (only) | 26 | 30 | 30 | 30 | 30 |
| Food intake (g/g) | 1080.0 | 1250.4 | 1262.4 | 1212.6 | 1256.1 |
| Average daily feed intake (g/day) | 41.54 | 41.68 | 41.96 | 40.42 | 41.87 |
| Average daily gain (g/day) | 21.95 | 22.07 | 22.38 | 23.30 | 22.29 |
| Feed to meat ratio | 1.892 | 1.888 | 1.875 | 1.735 | 1.878 |
The invention provides a fermentation traditional Chinese medicine preparation for inhibiting bacteria and promoting growth, which is prepared by fermenting 4 probiotics including bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae, wherein the bacillus and the saccharomycetes produce enzymes to promote the precipitation of tannins; lactobacillus plantarum and enterococcus faecium are fermented by using the traditional Chinese medicine mixture and fermentation auxiliary materials to produce antibacterial substances such as lactic acid and the like. The fermented Chinese medicinal preparation has inhibiting effect on common intestinal pathogenic bacteria, especially Staphylococcus aureus. The fermented traditional Chinese medicine preparation can promote the growth of broiler chickens and improve the feed conversion rate.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (9)
1. The application of the fermentation traditional Chinese medicine preparation for inhibiting bacteria and promoting growth is characterized in that the fermentation traditional Chinese medicine preparation is applied to the preparation of a bacteriostatic agent for inhibiting staphylococcus aureus;
the antibacterial growth-promoting fermentation traditional Chinese medicine preparation is prepared by fermenting a traditional Chinese medicine composition and probiotics;
the traditional Chinese medicine composition consists of gallnut, pericarpium Granati and oak bowl;
the probiotics consist of bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae;
the traditional Chinese medicine composition comprises the following components in parts by weight: 20-50 parts of gallnut, 20-30 parts of pomegranate rind and 10-20 parts of acorn bowl.
2. The use of a bacteriostatic growth-promoting fermented traditional Chinese medicine preparation according to claim 1, characterized in that the preparation method of the bacteriostatic growth-promoting fermented traditional Chinese medicine preparation comprises the following steps:
(1) Inoculating the bacillus subtilis, the lactobacillus plantarum, the enterococcus faecium and the saccharomyces cerevisiae into a sterilized independent culture medium for culture, and respectively preparing a bacillus subtilis activating solution, a lactobacillus plantarum activating solution, an enterococcus faecium activating solution and a saccharomyces cerevisiae activating solution after heat preservation and activation;
(2) Weighing the components of the traditional Chinese medicine composition according to parts by weight, respectively crushing, uniformly mixing the components of the traditional Chinese medicine composition to obtain a first mixture, adding water into the first mixture, uniformly mixing to obtain a second mixture, introducing steam into the second mixture for steam heating treatment, and cooling to obtain the traditional Chinese medicine mixture;
(3) Uniformly mixing the traditional Chinese medicine mixture with fermentation auxiliary materials according to the proportion of 1 (0.2-0.3), and respectively inoculating bacillus subtilis activating solution, lactobacillus plantarum activating solution, enterococcus faecium activating solution and saccharomyces cerevisiae activating solution, and fermenting to obtain the antibacterial growth-promoting fermentation traditional Chinese medicine preparation.
3. The use of a fermented Chinese medicinal preparation for inhibiting bacteria and promoting growth according to claim 2, wherein in the step (3), the fermentation auxiliary material is any one of corn flour, bran or soybean meal.
4. The application of the antibacterial growth-promoting fermentation traditional Chinese medicine preparation according to claim 2, wherein in the step (1), bacillus subtilis, lactobacillus plantarum, enterococcus faecium and saccharomyces cerevisiae are respectively inoculated into a sterilized independent culture medium according to the inoculation amount of 1-2%, and are subjected to heat preservation and activation for 2 hours to respectively prepare bacillus subtilis activating solution, lactobacillus plantarum activating solution, enterococcus faecium activating solution and saccharomyces cerevisiae activating solution.
5. The use of a bacteriostatic growth-promoting fermented chinese medicinal preparation according to claim 2, characterized in that in step (2), 15-30 parts by weight of water is added to the first mixture.
6. The use of a fermented Chinese medicinal preparation for inhibiting bacteria and promoting growth according to claim 2, wherein in the step (2), the components of the Chinese medicinal composition are respectively weighed according to parts by weight, and are respectively crushed to 60-80 meshes.
7. The use of a fermented Chinese medicinal preparation for inhibiting bacteria and promoting growth according to claim 2, wherein in the step (2), the parameters of the steam heating treatment performed by introducing steam into the second mixture are as follows: raising the temperature of the materials to 80-85 ℃ and treating for 2h.
8. The use of a bacteriostatic growth-promoting fermented traditional Chinese medicine preparation according to claim 2, characterized in that in step (3), the parameters of the fermentation treatment are: fermenting at 37deg.C for 3-5 days.
9. The use of a fermented Chinese medicinal preparation for inhibiting bacteria and promoting growth according to claim 2, wherein in the step (3), the addition amounts of the bacillus subtilis activating solution, the lactobacillus plantarum activating solution, the enterococcus faecium activating solution and the saccharomyces cerevisiae activating solution are respectively (0.1-0.5)%, (0.1-0.5)%, and (0.1-0.5)% of the weight of the Chinese medicinal mixture.
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| CN106798862A (en) * | 2017-01-16 | 2017-06-06 | 河南牧业经济学院 | A kind of Chinese medicine microecological preparation, preparation method for improving chicken immunity and usage thereof |
| CN108653518A (en) * | 2018-05-24 | 2018-10-16 | 湖北华大瑞尔科技有限公司 | A kind of probiotics fermention Chinese medicine preparation and preparation method thereof |
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| CN103549141A (en) * | 2013-04-25 | 2014-02-05 | 北京生泰尔生物科技有限公司 | Chinese herbal medicine composite probiotic feed additive for pigs and preparation method of additive |
| CN106798862A (en) * | 2017-01-16 | 2017-06-06 | 河南牧业经济学院 | A kind of Chinese medicine microecological preparation, preparation method for improving chicken immunity and usage thereof |
| CN108653518A (en) * | 2018-05-24 | 2018-10-16 | 湖北华大瑞尔科技有限公司 | A kind of probiotics fermention Chinese medicine preparation and preparation method thereof |
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| 橡子壳主要成分的生理功能及开发利用研究;张博;食品工业科技;第43卷(第7期);393-398 * |
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