CN107011411B - Cottonseed protein production method capable of extracting multiple byproducts - Google Patents
Cottonseed protein production method capable of extracting multiple byproducts Download PDFInfo
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- CN107011411B CN107011411B CN201710293807.2A CN201710293807A CN107011411B CN 107011411 B CN107011411 B CN 107011411B CN 201710293807 A CN201710293807 A CN 201710293807A CN 107011411 B CN107011411 B CN 107011411B
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
Abstract
A method for producing cottonseed protein capable of extracting a plurality of byproducts comprises the following steps: stirring and extracting the meal under the action of a solvent, wherein the solvent is a mixed solvent of a methanol solution and a vegetable oil extraction solvent; separating the material from the solvent after extraction to obtain wet meal; desolventizing wet meal; drying the wet meal at low temperature; adjusting the water content in the wet meal, and continuously removing the residual solvent to obtain a cottonseed concentrated protein product meeting the requirements; separating the separated solvent to respectively obtain a methanol mixed solution and cottonseed mixed oil containing the vegetable oil extraction solvent; continuously separating methanol mixed liquor, recovering methanol, and taking the rest as a mixture of crude sugar and crude fat; separating the mixture of crude sugar and crude lipid to obtain crude sugar and crude lipid. The method uses cottonseed meal produced by low-temperature processing as a raw material, can obtain concentrated cottonseed protein and crude sugar after extraction, can also obtain crude lipid substances exceeding the yield of the crude sugar, and can further extract cottonseed oil, so that residual oil in the meal is further reduced, and the cottonseed processing field has better economy.
Description
Technical Field
The invention relates to the technical field of cottonseed processing, in particular to a method for producing cottonseed protein, which can obtain concentrated cottonseed protein, crude sugar substances and crude lipid substances after processing.
Background
The cottonseed protein belongs to bean protein, has the characteristics of low fiber, high metabolic energy, high amino acid digestibility, good palatability and the like, and is high-quality edible protein. However, because cotton seeds contain gossypol, which is a toxic substance, complete dephenolation, increased cottonseed protein content after treatment, and reduced levels of various sugars and other anti-nutritional factors remaining in the cottonseed protein are of concern to the industry.
The invention patent application with application number 201210483256.3 discloses a cottonseed treatment method, which comprises the steps of sequentially carrying out extraction, dehumidification, drying and other treatments on oil-extracted cottonseed, and extracting a large amount of substances such as gossypol, raffinose and other sugars by a low-concentration and large-dosage methanol solution soaking mode to ensure that the content of concentrated protein of the cottonseed reaches more than 65%.
The problems existing in the process are as follows: substances extracted from methanol, except gossypol, saccharides and the like, still contain a large amount of crude lipid substances in protein materials, and are not extracted; the method adopts a single polar solvent alcohol solvent, can only extract the anti-nutritional factors dissolved in the polar solvent, and has no effect on the anti-nutritional substances and lipid substances dissolved in the non-polar solvent. Therefore, how to obtain beneficial substances in the cottonseed as much as possible, reduce various anti-nutritional factors remained in the cottonseed meal, and greatly improve the metabolic energy of the protein and the amino acid digestibility becomes a problem concerned by the patent application.
Disclosure of Invention
The invention provides a method for producing cottonseed protein, which can comprehensively extract substances dissolved in organic solvent, polar solvent and water in cottonseed meal to obtain concentrated cottonseed protein with high digestion energy, metabolic energy and amino acid digestibility, and adopts the technical scheme that: a method for producing cottonseed protein capable of extracting a plurality of byproducts is characterized in that: the production method comprises the following steps:
1) stirring and extracting the meal under the action of a solvent, wherein the solvent is a mixed solvent of a methanol solution and a vegetable oil extraction solvent;
2) separating the material from the solvent after extraction to obtain wet meal;
3) desolventizing wet meal;
4) drying the wet meal at low temperature;
5) adjusting the water content in the meal, and continuously removing the residual solvent to obtain a cottonseed concentrated protein product meeting the requirements;
6) separating the mixed liquor separated in the step 2) and the step 3) to respectively obtain a methanol mixed liquor and cottonseed mixed oil containing a vegetable oil extraction solvent;
7) continuously separating the methanol mixed liquor in the step 6), recovering methanol, and taking the rest as a mixture of crude sugar and crude fat;
8) separating the mixture of crude sugar and crude lipid to obtain crude sugar and crude lipid. The invention is also characterized in that: and separating the cottonseed mixed oil containing the vegetable oil extraction solvent to obtain crude cottonseed oil.
The invention is also characterized in that: the methanol solution in the step 1) is 75-90% methanol solution, and the mixing proportion of the methanol and the vegetable oil extraction solvent in the mixed solvent is (5-9): (1- -5).
The invention is also characterized in that: in the step 1), the extraction temperature is 30-55 ℃, and the ratio of meal powder to mixed solvent is less than or equal to 0.25.
The invention is also characterized in that: in the step 2), the extracted material is dried into wet meal;
the invention is also characterized in that: and 3) further extruding and desolventizing the wet meal, wherein the moisture content of the extruded wet meal is less than or equal to 40%.
The invention is also characterized in that: and 4) drying at a low temperature in the step 4), wherein the temperature of the materials is 80-90 ℃.
The invention is also characterized in that: in the step 5), the moisture content of the meal is adjusted by adopting a humidifier, so that the residual solution is further reduced, and the moisture content is less than or equal to 8%;
the invention is also characterized in that: and 7) separating the methanol mixed solution in the step 7) by adopting a thin film evaporation separation method.
The invention is also characterized in that: the cottonseed meal is obtained by crushing cottonseed low-temperature cake meal to the particle size of less than or equal to 3mm, wherein the cottonseed low-temperature cake meal is cottonseed primary leaching meal, cottonseed low-temperature machine pressed cake or cottonseed low-temperature puffing leached meal.
The invention has the beneficial effects that: the method uses cottonseed meal produced by low-temperature processing as a raw material, adopts mixed solvent extraction, can obtain concentrated cottonseed protein and crude sugar with higher protein content after extraction, can also obtain crude lipid substances with yield exceeding that of the crude sugar (the yield of the crude sugar is less than or equal to 1 percent, the yield of the crude lipid is more than or equal to 1.5 percent, and the yield takes the smooth cottonseed as the base number), and can further extract the cottonseed oil, so that the residual oil in the meal is further reduced (less than or equal to 0.5 percent), and the cottonseed oil processing field has better economy.
Drawings
FIG. 1 is a process flow diagram of the present invention.
Detailed Description
The following describes a specific embodiment of the present invention with reference to the drawings.
The first embodiment is as follows: a method for producing cottonseed protein capable of extracting a plurality of byproducts comprises the following steps:
1) the method comprises the following steps of stirring and extracting the cottonseed meal under the action of a solvent, wherein the solvent is a mixed solvent of a methanol solution and a vegetable oil extraction solvent, the cottonseed meal is obtained by crushing cottonseed low-temperature meal to the particle size of less than or equal to 3mm, the cottonseed low-temperature meal is cottonseed primary leaching meal, and the raw material is crushed to the particle size of less than or equal to 3 mm. The concentration of a methanol solution for preparing the mixed solvent is 75-90%, the mixing ratio of methanol to the vegetable oil extraction solvent in the mixed solvent is 8:2, the mixed solvent with the ratio is used for extracting the once-leached meal, the extraction temperature is 30-55 ℃, and the ratio of the meal powder to the solvent or the ratio of the meal powder to the mixed solvent is less than or equal to 0.25.
2) Separating the material from the mixed solution after extraction, and drying the material to obtain wet meal;
3) desolventizing the wet meal, further extruding the wet meal for desolventizing, wherein the moisture content of the extruded wet meal is less than or equal to 40 percent;
4) and (3) drying the wet meal at a low temperature, wherein the temperature of the low-temperature dried material is 80-90 ℃.
5) Adjusting the water content of the wet meal, and continuously removing the residual solvent to obtain a cottonseed concentrated protein product meeting the requirement, wherein the water content of the meal can be adjusted by adopting a humidifier and is less than or equal to 8%; the fineness of the obtained protein product is 80 meshes and the passing rate is 90 percent.
6) Separating the mixed liquor separated in the step 2) and the step 3) to respectively obtain a methanol mixed liquor and cottonseed mixed oil containing a vegetable oil extraction solvent;
7) continuously separating the methanol mixed liquor in the step 6), recovering methanol, and taking the rest as a mixture of crude sugar and crude fat; and 7) separating the methanol mixed solution in the step 7) by adopting a thin film evaporation separation method.
8) Separating the mixture of crude sugar and crude lipid to obtain crude sugar and crude lipid.
The crude cottonseed oil is obtained by separating the cottonseed mixed oil containing the vegetable oil extraction solvent.
The process method is used for leaching the meal for the first time, so that a concentrated cottonseed protein product with the protein content of more than or equal to 65 percent (dry basis) can be obtained, and the residual oil is reduced to 0.5 percent; the yield of crude sugar (based on cottonseed) is more than or equal to 1.2%; the yield of crude fat (based on the cottonseed oil) is more than or equal to 1.6 percent.
Example two:
1) the cottonseed meal is obtained by crushing cottonseed low-temperature cakes to the particle size of less than or equal to 3mm, wherein the cottonseed low-temperature cakes are cottonseed cakes processed by low-temperature mechanical pressing of the cottonseed, and the raw materials are crushed to the particle size of less than or equal to 3 mm. The concentration of a methanol solution for preparing the mixed solvent is 80%, the mixing ratio of methanol to the vegetable oil extraction solvent in the mixed solvent is 6:4, the mixed solvent with the ratio is used for extracting low-temperature machine-pressed cakes, the extraction temperature is 30-55 ℃, and the ratio of meal powder to solvent or the ratio of the meal powder to the mixed solvent is less than or equal to 0.25.
2) Separating the material from the mixed solution after extraction, and obtaining wet meal after the material is dried;
3) desolventizing the wet meal, further extruding the wet meal for desolventizing, wherein the moisture content of the extruded wet meal is less than or equal to 40 percent;
4) and (3) drying the wet meal at a low temperature, wherein the temperature of the material dried at the low temperature is 80-90 ℃.
5) Adjusting the water content in the wet meal, continuously removing the residual solvent to obtain a cottonseed concentrated protein product meeting the requirement, and adjusting the water content by adopting a humidifier, wherein the water content of the product is less than or equal to 7%; the fineness of the obtained protein product is 80 meshes and the passing rate is 90 percent.
6) Separating the mixed liquor separated in the step 2) and the step 3) to respectively obtain a methanol mixed liquor and cottonseed mixed oil containing a vegetable oil extraction solvent;
7) continuously separating the methanol mixed liquor in the step 6), recovering methanol, and taking the rest as a mixture of crude sugar and crude fat; and 7) separating the methanol mixed solution in the step 7) by adopting a thin film evaporation separation method.
8) Separating the mixture of crude sugar and crude lipid to obtain crude sugar and crude lipid.
The cottonseed oil is obtained by separating the cottonseed mixed oil containing the vegetable oil extraction solvent.
The cottonseed protein concentrate is used for low-temperature pressed cakes (taking the oil content of the low-temperature pressed cakes is less than or equal to 7 percent, taking 7 percent as an example), the proportion of the adopted mixed solvent is 6:4, and a cottonseed protein concentrate product with the protein content of more than or equal to 65 percent (dry basis) can be obtained; the yield of crude sugar (based on cottonseed) is more than or equal to 1.2%; the yield of crude fat (based on the smooth cottonseed) is more than or equal to 1.6 percent; and 6% of cottonseed oil extraction.
Adjusting the proportion of the mixed solvent to 5:5, adopting a low-temperature machine-pressed cake (containing oil less than or equal to 12 percent, taking 12 percent as an example) as a raw material, and obtaining a cottonseed concentrated protein product with the protein content of more than or equal to 65 percent (dry basis) by adopting the process; the yield of crude sugar (based on cottonseed) is more than or equal to 1.2%; the yield of crude fat (based on the smooth cottonseed) is more than or equal to 1.8 percent; and extracting cottonseed oil by 11%.
Example three:
1) the method comprises the following steps of stirring and extracting the cottonseed meal under the action of a solvent, wherein the solvent is a mixed solvent of a methanol solution and a vegetable oil extraction solvent, the cottonseed meal is obtained by crushing cottonseed low-temperature cakes to the particle size of less than or equal to 3mm, the cottonseed low-temperature cakes are cottonseed cakes processed by the cottonseed low-temperature expanded oil-extracted cakes, and the raw materials are crushed to the particle size of less than or equal to 3 mm. The concentration of a methanol solution for preparing the mixed solvent is 90%, the mixing ratio of methanol to the vegetable oil extraction solvent in the mixed solvent is 9:1, the mixed solvent with the ratio is used for extracting the cottonseed meal after low-temperature puffing oil extraction, the extraction temperature is 30-55 ℃, and the ratio of the cottonseed meal to the solvent or the mixed solvent is less than or equal to 0.25.
2) Separating the material from the mixed solution after extraction, and drying the material to obtain wet meal;
3) desolventizing the wet meal, further extruding the wet meal for desolventizing, wherein the moisture content of the extruded wet meal is less than or equal to 40 percent;
4) and (3) drying the wet meal at a low temperature, wherein the temperature of the low-temperature dried material is 80-90 ℃.
5) Adjusting the water content in the meal, continuously removing the residual solvent to obtain a cottonseed concentrated protein product meeting the requirement, and adjusting the water content by adopting a humidifier, wherein the water content of the product is less than or equal to 6.5%; the fineness of the obtained protein product is 80 meshes and the passing rate is 90 percent.
6) Separating the mixed liquor separated in the step 2) and the step 3) to respectively obtain a methanol mixed liquor and cottonseed mixed oil containing a vegetable oil extraction solvent;
7) continuously separating the methanol mixed liquor in the step 6), recovering methanol, and taking the rest as a mixture of crude sugar and crude fat; and 7) separating the methanol mixed solution in the step 7) by adopting a thin film evaporation separation method.
8) Separating the mixture of crude sugar and crude lipid to obtain crude sugar and crude lipid.
The cottonseed oil is obtained by separating the cottonseed mixed oil containing the vegetable oil extraction solvent.
The cottonseed protein concentrate is used for low-temperature puffing of the oil-extracted meal to obtain a cottonseed protein concentrate product with the protein content of more than or equal to 65% (dry basis); the yield of crude sugar (based on cottonseed) is more than or equal to 1.2%; the yield of crude fat (based on the smooth cottonseed) is more than or equal to 1.8 percent; the protein residual oil is less than or equal to 0.3 percent.
Claims (8)
1. A method for producing cottonseed protein capable of extracting a plurality of byproducts is characterized in that: the production method comprises the following steps:
1) stirring and extracting the meal under the action of a solvent, wherein the solvent is a mixed solvent of a methanol solution and a vegetable oil extraction solvent;
2) separating the material from the solvent after extraction to obtain wet meal;
3) desolventizing wet meal;
4) drying the wet meal at low temperature;
5) adjusting the water content in the meal, and continuously removing the residual solvent to obtain a cottonseed concentrated protein product meeting the requirements;
6) separating the mixed liquor separated in the step 2) and the step 3) to respectively obtain a methanol mixed liquor and cottonseed mixed oil containing a vegetable oil extraction solvent;
7) continuously separating the methanol mixed liquor in the step 6), recovering methanol, and taking the rest as a mixture of crude sugar and crude fat;
8) separating the mixture of the crude sugar and the crude fat to obtain crude sugar substances and crude fat substances;
the methanol solution in the step 1) is 75-90% methanol solution, and the mixing proportion of the methanol and the vegetable oil extraction solvent in the mixed solvent is (8-9): (1-2);
in the step 1), the extraction temperature is 30-55 ℃, and the ratio of meal powder to mixed solvent is less than or equal to 0.25.
2. The cottonseed protein production method of claim 1, wherein: and separating the cottonseed mixed oil containing the vegetable oil extraction solvent to obtain crude cottonseed oil.
3. The cottonseed protein production method of claim 1, wherein: in the step 2), the extracted materials are dried to form wet meal.
4. The cottonseed protein production method of claim 1, wherein: and 3) extruding the wet meal for further desolventizing, wherein the moisture content of the extruded wet meal is less than or equal to 40%.
5. The cottonseed protein production method of claim 1, wherein: and 4) drying at a low temperature in the step 4), wherein the temperature of the materials is 80-90 ℃.
6. The cottonseed protein production method of claim 1, wherein: and 5) adjusting the water content of the meal by adopting a humidifier to further reduce the residual solvent, wherein the water content is adjusted to be less than or equal to 8%.
7. The cottonseed protein production method of claim 1, wherein: and 7) separating the methanol mixed solution in the step 7) by adopting a thin film evaporation separation method.
8. The cottonseed protein production method of claim 1, wherein: the cottonseed meal is obtained by crushing cottonseed low-temperature cake meal to the particle size of less than or equal to 3mm, wherein the cottonseed low-temperature cake meal is cottonseed primary leaching meal, cottonseed low-temperature machine-pressed cake or cottonseed low-temperature puffing oil extraction meal.
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CN110833115A (en) * | 2019-11-21 | 2020-02-25 | 南京胜昔实业有限公司 | Comprehensive utilization method of cotton production by-products by using biochemical fulvic acid as main product |
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CN111406828A (en) * | 2020-03-30 | 2020-07-14 | 山东凯斯达机械制造有限公司 | Device and process for extracting soybean effective components by using mixed solvent |
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