CN107007635A - A kind of preparation method of the anti-viruses of EV 71 of moss active component - Google Patents
A kind of preparation method of the anti-viruses of EV 71 of moss active component Download PDFInfo
- Publication number
- CN107007635A CN107007635A CN201710402467.2A CN201710402467A CN107007635A CN 107007635 A CN107007635 A CN 107007635A CN 201710402467 A CN201710402467 A CN 201710402467A CN 107007635 A CN107007635 A CN 107007635A
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- CN
- China
- Prior art keywords
- moss
- antiviral
- active component
- precipitation
- alcohol precipitation
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Links
- 230000002155 anti-virotic effect Effects 0.000 title claims abstract description 8
- 238000002360 preparation method Methods 0.000 title description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 34
- 230000000840 anti-viral effect Effects 0.000 claims abstract description 22
- 238000001556 precipitation Methods 0.000 claims abstract description 16
- 238000000605 extraction Methods 0.000 claims abstract description 12
- 238000000926 separation method Methods 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000011347 resin Substances 0.000 claims abstract description 8
- 229920005989 resin Polymers 0.000 claims abstract description 8
- 238000009395 breeding Methods 0.000 claims abstract description 6
- 230000001488 breeding effect Effects 0.000 claims abstract description 6
- 239000003480 eluent Substances 0.000 claims abstract description 6
- 239000000284 extract Substances 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims abstract description 5
- 241000700605 Viruses Species 0.000 claims description 6
- 239000008346 aqueous phase Substances 0.000 claims description 4
- 239000004480 active ingredient Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 claims description 3
- 238000011160 research Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 230000006837 decompression Effects 0.000 claims description 2
- 238000000746 purification Methods 0.000 claims description 2
- 238000000967 suction filtration Methods 0.000 claims description 2
- 125000003158 alcohol group Chemical group 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 4
- 230000002745 absorbent Effects 0.000 abstract description 3
- 239000002250 absorbent Substances 0.000 abstract description 3
- 238000012216 screening Methods 0.000 abstract description 3
- 230000003612 virological effect Effects 0.000 abstract description 2
- 238000003809 water extraction Methods 0.000 abstract description 2
- 208000020061 Hand, Foot and Mouth Disease Diseases 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 208000025713 Hand-foot-and-mouth disease Diseases 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 241000195940 Bryophyta Species 0.000 description 2
- 241000709661 Enterovirus Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 208000030090 Acute Disease Diseases 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 208000006740 Aseptic Meningitis Diseases 0.000 description 1
- 241000195896 Bryales Species 0.000 description 1
- 208000014912 Central Nervous System Infections Diseases 0.000 description 1
- 241000195889 Funariaceae Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010027201 Meningitis aseptic Diseases 0.000 description 1
- 241000921453 Rhodobryum Species 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000002567 autonomic effect Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 208000030194 mouth disease Diseases 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/10—Bryophyta (mosses)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the separation of the extraction process of effective component of chinese medicine and antivirus effective position, the separation method of the viral active components of the predominantly anti-EV of moss extract 71, concretely comprise the following steps using moss water extraction, alcohol precipitation is antiviral, dissolving precipitation, the macroporous absorbent resin of DM130 models is splined on, the eluent of various concentrations connects one bottle per 200ml, each concentration gradient is eluted to colourless and concentrated, and each eluent carries out Antiviral breeding.After screening, the extract of moss carries out separation antiviral effect preferably through water, determines antivirus effective position.The present invention is found that moss antivirus effective position using the method for antiviral tracking, and antiviral effect is preferable.
Description
Technical field
Extraction process is produced the present invention relates to active ingredient of Chinese herbs, is related to the new approaches of moss antivirus effective position extraction
New method and the new application in pharmaceutical field, and in particular to utilize the anti-intestines of mode extraction separation and purification moss of antiviral tracking
The antiviral research of road 71 type active ingredients of virus.
Background technology
The Chinese herbal medicine resource of China enriches, and cheap, and extensively, significantly, adverse reaction is small for pharmacological action for medicine source, with
The technology continuous improvement of screening, extracting and developing, purifying, research has found the composition with antiviral activity from Chinese medicine
One of important channel as exploitation antiviral drugs.
Moss is bryophyte, belongs to Bryophyta moss guiding principle Bryales Funariaceae cucurbit Rhodobryum, is grown in darkness
On moist ground, wall, in well, in roofing tile and water etc..
Hand-foot-and-mouth disease（Hand-Foot-Mouth Disease,HFMD）It is the acute disease of the comparison triggered by enterovirus
Toxoinfection disease, most hand-foot-and-mouth disease patient is because infection enterovirns type 71（Entervirus 71, EV71）Drawn
Rise.EV71 is to have now been found that enterovirus the latest, and EV71 viral harmfulness is very strong, mainly causes infant（0~6
Year）Hand-foot-and-mouth disease, additionally it is possible to cause a variety of diseases related to nervous system, such as aseptic meningitis also has BBE
Deng.Hand-foot-and-mouth disease and central nervous system infection are two big common symptoms caused by EV71 infection.
Moss extract has the effect of anti-enterovirns type 71.
Moss taste is micro- salty, cold in nature nontoxic, enters Liver Channel, consults related data, and moss has anti-inflammation, clearing heat and detoxicating town
Bitterly, effect of cool blood dissipates the stasis of blood.Study less in anti-virus aspect at present, be a kind of wide autonomic drug of potentiality to be exploited.
The content of the invention
The technical problems to be solved by the invention are the active components that specify that the anti-EV-71 viruses of moss, for effective portion
The separation and Extraction of position provides the new method that a kind of mode using antiviral tracking prepares moss antivirus effective position.
It is an advantage of the invention that the present invention has isolated a kind of active component in moss with anti-EV-71 viruses first,
And invented a kind of method of antiviral tracking prepared by the active component, this separation method purpose is strong, simple section
Substantial amounts of manpower and materials have been saved, have been separated better than traditional system.The active component prepared has preferable to EV-71 viruses
Therapeutic action.
The antiviral preparation method of moss active component of the present invention, comprises the following steps：
（1）Take a certain amount of moss water extraction；
（2）Extract solution is concentrated under reduced pressure, the medicinal material that every milliliter of decoction contains 1 gram is concentrated into, then adds the dense of ethanol regulation ethanol
Spend for 70%, standing 24 hours.It is filtrated to get precipitation and supernatant；
（3）Supernatant is reclaimed into ethanol, precipitation water is dissolved, Antiviral breeding is carried out respectively.It is disease-resistant that experiment display is precipitated
Toxic effect fruit is preferable；
（4）Take certain DM130 models macroporous absorbent resin to be soaked 24 hours with 95% ethanol respectively, post is filled afterwards, with distillation
No alcohol taste is washed to, 3 hours of immersion are then rinsed with the aqueous hydrochloric acid solution of 5 times of column volumes 5%, are rushed afterwards with distilled water
It is equal to 7 to PH, then 3 hours of immersion is rinsed with the 5% NaOH aqueous solution of 5 times of column volumes, is rushed afterwards with distilled water to PH
It is standby equal to 7；
（5）Take（3）In precipitation be splined on the macroporous resin column handled well, respectively with water, 25% ethanol, 50% ethanol, 75% ethanol
It is rinsed, one bottle is connect per 200ml in each eluent, and is flushed to after no color and changes a kind of eluent again；
（6）The above-mentioned sample being connected to is subjected to Antiviral breeding.Experiment display is by the screening of macroreticular resin, the aqueous phase portion of moss
Position antiviral effect is preferable.
Embodiment
Example 1（1）A certain amount of moss is weighed, aqueous extract is concentrated under reduced pressure into the medicinal material that every milliliter of decoction contains 1 gram,
Plus the concentration of ethanol regulation solution ethanol is 70%, stands 24 hours；
（2）Will（1）Obtained precipitation solution decompression suction filtration, is precipitated, by precipitation distilled water ultrasonic dissolution；
（3）Will（2）Obtained solution is splined on the DM130 macroporous absorbent resins handled well, is carried out with the ethanol of water, various concentrations
Rinse, one bottle is connect per 200ml, rinse to nothing；
（4）Merge（3）The ethanol position of reclaimed water, various concentrations, is concentrated into certain volume；
（5）Will（4）In obtain moss difference elution position carry out Antiviral breeding respectively, as a result show aqueous phase moss extraction
Thing antiviral effect is preferable.
Claims (6)
1. a kind of moss extract has the active component and a kind of mode using antiviral tracking of anti-EV-71 virus functions
The process of the extraction separation and purification active ingredient, it is characterised in that the precipitation position of moss water extract-alcohol precipitation, which has, suppresses EV-
The effect of 71 viruses, first with alcohol precipitation, is secondly separated using the macroreticular resin of DM130 models, is separated in the extraction of each step
The antiviral place for tracking out active component is carried out afterwards, finally carried out Antiviral breeding research, drawn preferred plan.
2. active component according to claim 1 is characterized in that the precipitation position that the active component is alcohol precipitation, through macropore
After resin separation, second bottle of aqueous phase is the antiviral active component of moss.
3. extraction separation method according to claim 1, it is characterised in that the concentration concentration of moss is 1g/ml, alcohol precipitation concentration
For 70%, the alcohol precipitation time is 24 hours.
4. extraction separation method according to claim 1, it is characterised in that decompression suction filtration is carried out after alcohol precipitation, obtain precipitation and
Supernatant, then carries out Antiviral breeding and obtains being precipitated as antivirus effective position.
5. extraction separation method according to claim 1, it is characterised in that the macroreticular resin model DM130 used in moss,
Eluent is water, and 25% ethanol, 50% ethanol, 75% ethanol connects one bottle, each gradient is rushed to colourless per 200ml.
6. extraction separation method according to claim 1, it is characterised in that carry out the eluent of moss extract antiviral
Experiment, obtain aqueous phase second bottle is active component.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710402467.2A CN107007635A (en) | 2017-06-01 | 2017-06-01 | A kind of preparation method of the anti-viruses of EV 71 of moss active component |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710402467.2A CN107007635A (en) | 2017-06-01 | 2017-06-01 | A kind of preparation method of the anti-viruses of EV 71 of moss active component |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107007635A true CN107007635A (en) | 2017-08-04 |
Family
ID=59450994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710402467.2A Pending CN107007635A (en) | 2017-06-01 | 2017-06-01 | A kind of preparation method of the anti-viruses of EV 71 of moss active component |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107007635A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103583735A (en) * | 2013-10-22 | 2014-02-19 | 朱霞 | Seasoning mushroom tea electuary and preparation method thereof |
-
2017
- 2017-06-01 CN CN201710402467.2A patent/CN107007635A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103583735A (en) * | 2013-10-22 | 2014-02-19 | 朱霞 | Seasoning mushroom tea electuary and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 王清等: "藿香抗肠道EV-71病毒的体外筛选", 《辽宁中医杂志》 * |
| 王玎: "《耳鼻咽喉常见疾病的诊断与防治》", 28 February 2001 * |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170804 |