CN101033445B - Wine capable of protecting liver and preserving one's health and preparing method thereof - Google Patents
Wine capable of protecting liver and preserving one's health and preparing method thereof Download PDFInfo
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- CN101033445B CN101033445B CN200610130679A CN200610130679A CN101033445B CN 101033445 B CN101033445 B CN 101033445B CN 200610130679 A CN200610130679 A CN 200610130679A CN 200610130679 A CN200610130679 A CN 200610130679A CN 101033445 B CN101033445 B CN 101033445B
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Abstract
This invention discloses a liver-protection and healthy wine and its preparation method, in which, the wine includes distilled spirit, puerarin, glycyrrhizic acid, resveratrol, paeoniflorin, march 7 total glycosides and salvia acid B, and the spirit includes mixtures of one or several kinds of healthy factors, which avoids harm of spirit to the liver.
Description
Technical field
The present invention relates to food and protective foods, particularly relate to a kind of contain plant milk extract protect liver health wine and preparation method thereof.
Background technology
Liquor historical of long standing and well established is that people entertain, one of important drink when celebrating.The ethanol content of liquor is higher, and other nutritive substance relative content is lower, and is little to the human body benefit after drinking.Along with the progress of society, the improving constantly of people's living standard, idea is also being brought in constant renewal in, and when requiring to drink, can not only enjoy drink happy, and will guarantee healthy.At present, the number of drinking white spirit is accustomed to for a long time up to more than one hundred million by China.In this colony, because long-term drinking liquor, alcohol has caused infringement to its liver more or less, therefore, prevents from or alleviate alcohol the problem of the infringement of liver is put a great problem in the wine industry.
Liver is secretion and a detoxifcation organ maximum in the body, is all carrying out food digestion and detoxifcation all the time.Many objectionable impuritiess comprise medicine, and chemical substance etc. all will change into harmless material in liver.When liver function sustains damage for a long time, can threaten the health of human body.It has been more than 30 times of the eighties in 20th century that the change of dietary structure, excessive drinking cause fatty liver and alcoholic liver sickness rate.Fatty liver, alcoholic liver have become the major reason of hepatocellular injury.Fatty liver, alcoholic liver disease are second the common hepatopathys that is only second to viral hepatitis, are one of current social urgent problems, also lack specific methods of treatment at present.Therefore, in wine, dissolve in medicinal material with different health-care effecies or plurality of Chinese medicine materical crude slice and put into wine and soak and form, in order to prevention with reduce the damage of alcohol liver.This kind immersion process has been obtained certain effect, obtained the approval of partial mass, but also there is following shortcoming in this immersion process: quality product is wayward, and nutritive ingredient in the medicinal material and effective constituent can not rationally effectively be extracted, and the wine after soaking, color depth, bitter; Because coloured being difficult to when entertaining of wine enters dinner party wine, and mouthfeel is relatively poor, drink just as taking medicine, lost the aromatic of wine,, and added sugar often in order to solve sensory issues, still be difficult to be accepted, be unfavorable for alcohol user's health care to health when drinking by the people.
Summary of the invention
Technical problem to be solved by this invention is, overcomes the shortcoming of prior art, and a kind of crystalline powder that adds plant milk extract in wine is provided, and produces colourless clear saturating, pure taste, can relieve the effect of alcohol, detoxify can protect the liver, protect liver again protect liver health wine.
The technical solution used in the present invention is: a kind of liver health wine that protects comprises liquor and plant milk extract; Described plant milk extract comprises puerarin, Potenlini, trans-resveratrol, peoniflorin, Radix Notoginseng total glycosides, salvianolic acid B; Comprise one or more of plant milk extract in the described liquor, contain from 1mg to liquor in every 500ml wine the plant milk extract of maximum saturation solubleness.
The proportioning of plant milk extract and liquor (w/v) is 1mg～400mg/500ml.
Contain plant milk extract puerarin 30mg, salvianolic acid B 50mg, Potenlini 50mg, trans-resveratrol 10mg, peoniflorin 60mg in 500ml (38%) liquor.
Also comprise potassium, the sodium of Potenlini, the salt compounds of ammonium.
The liquor number of degrees are 0.5～65 degree.
A kind of preparation method who protects liver health wine comprises the steps:
(1) purification of plant milk extract
F. choose natural plant material: puerarin, salvianolic acid B, trans-resveratrol, peoniflorin and Potenlini;
G. raw material is carried out boiling, extraction, alcohol precipitation, concentrates the acquisition concentrated solution;
H. concentrated solution is passed through macroporous resin purification, described macroporous resin purification comprises: upper prop, absorption and wash-out;
I. water and 10%～90% ethanol wash-out macroporous adsorbent resin is respectively successively collected elutriant, the dry crude product that obtains;
J. the purifying plant milk extract obtains the effective constituent compound monomer and protects liver plant milk extract white crystalline powder;
(2) protect the finished product method of liver health wine
In the plant milk extract behind the above-mentioned E item gained purifying one or more are put into storage wine jar in proportion, take out with wine by pump repeatedly and fully merge, behind molecular sieve filtration, obtain protecting liver health wine.
The invention has the beneficial effects as follows: the present invention protects color and the taste that liver health bartender has stayed original liquor, and colourless or little yellow, clear saturating, free from extraneous odour have the sweet-smelling of wine, and health factor are that plant milk extract is clear and definite, stable and controllable for quality; The health factor that is added does not contain any chemical additive component, is a kind of green food and healthcare products of environment-friendly type, and Chinese medicine content of active substance wherein has prevention and alleviates the effect of alcohol to the infringement of liver.Owing in wine, added new healthy element, make the colony of getting used to drinking for a long time, the health factor that can will have liver protection function in daily drinking is sent in the body, reach the formation that effectively prevents or control alcoholic liver, fatty liver, in the process of drinking, realize the health care, promote longevity.This health wine is clear thoroughly, sweet-smelling is strong can also have higher popularization and application values as dinner party wine.
Description of drawings
Fig. 1 is preparation method's schema that the present invention protects liver health wine.
Embodiment
Protect liver health wine, comprise liquor and plant milk extract; Described plant milk extract comprises puerarin, Potenlini, trans-resveratrol, peoniflorin, Radix Notoginseng total glycosides, salvianolic acid B; Comprise one or more mixture of plant milk extract in the described liquor, contain from 1mg to liquor in every 500ml wine the plant milk extract of maximum saturation solubleness.The proportioning of plant milk extract and liquor (w/v) is 1mg～400mg/500ml.Can contain plant milk extract (active ingredient of Chinese herbs) puerarin 1～300mg, trans-resveratrol 1～200mg, peoniflorin 1～396mg, Potenlini 1～200mg, salvianolic acid B 1～300mg in the general 500ml liquor.The comparatively tangible mix proportion scheme of health-care effect also can adopt and contain plant milk extract (active ingredient of Chinese herbs) puerarin 30～300mg, trans-resveratrol 30～200mg, peoniflorin 50～330mg, Potenlini 30～200mg, salvianolic acid B 30～300mg in 500ml liquor.The mix proportion scheme of best health-care effect is for dissolving in active ingredient of Chinese herbs puerarin 30mg, salvianolic acid B 50mg, Potenlini 50mg, trans-resveratrol 10mg, peoniflorin 60mg in 500ml liquor (38%).Protecting the liquor number of degrees that liver health wine adopted can be 0.5～65 degree, and the preferred number of degrees are 38 degree.Protect the salt compounds of potassium that liver health wine can also comprise edible Potenlini, sodium, ammonium.
Below the preparation and the detection method of protecting liver health wine illustrated:
(1) respectively to contain health factor of the present invention be the integration of drinking and medicinal herbs of plant milk extract or natural phant extraction, separation, purifying such as the macroporous adsorption resin technology that is used for protective foods and chromatogram purification technical point from, to prepare the effective constituent compound monomer with hepatoprotective effect that its purity reaches more than 90% be health factor.
(2), make new type health health wine and should meet following characteristics with a kind of health factor of the present invention or severally dissolve in the liquor by prescription: colourless or little yellow, limpid bright, free from extraneous odour, have the sweet-smelling of wine, health factor is clear and definite, stable and controllable for quality.
(3) adopt quantitatively the Quality Control Technology of measuring health factor content respectively of HPLC method accurately:
1. the HPLC method is measured the method for Potenlini:
Chromatographic condition
Moving phase: acetonitrile-0.1mol/L ammonium acetate solution-Glacial acetic acid (29: 71: 0.5);
Chromatographic column: C
18(5um), filler is kromasil;
Detect wavelength: 250nm;
The preparation of reference substance solution: it is an amount of that precision is measured the Potenlini reference substance, makes the reference substance solution that every 1ml contains Potenlini 80ug with 50% methyl alcohol.
Sample solution preparation: measure sample solution 5ml, place the 25ml measuring bottle, add 50% methyl alcohol and be diluted to scale, shake up, promptly.
Assay method: precision is measured reference substance solution and each 10～20ul of sample solution respectively, injects the HPLC instrument, measures, and calculates by external standard method, promptly.
2. the HPLC method is measured the method for puerarin:
Chromatographic condition
Moving phase: methanol-water (25: 75);
Chromatographic column: C
18(5um), filler is kromasil;
Detect wavelength: 250nm;
The preparation of reference substance solution: it is an amount of that precision is measured the puerarin reference substance, makes the reference substance solution that every 1ml contains puerarin 40ug with 50% methyl alcohol.
Sample solution preparation: measure sample solution 5ml, place the 25ml measuring bottle, add 50% methyl alcohol and be diluted to scale, shake up, promptly.
Assay method: precision is measured reference substance solution and each 10～20ul of sample solution respectively, injects the HPLC instrument, measures, and calculates by external standard method, promptly.
3. the HPLC method is measured the method for peoniflorin:
Chromatographic condition moving phase: methanol-water (25: 75);
Chromatographic column: C
18(5um), filler is kromasil;
Detect wavelength: 270nm;
The preparation of reference substance solution: it is an amount of that precision is measured the peoniflorin reference substance, makes the reference substance solution that every 1ml contains peoniflorin 50ug with 50% methyl alcohol.
Sample solution preparation: measure sample solution 5ml, place the 25ml measuring bottle, add 50% methyl alcohol and be diluted to scale, shake up, promptly.
Assay method: precision is measured reference substance solution and each 10～20ul of sample solution respectively, injects the HPLC instrument, measures, and calculates by external standard method, promptly.
Preparation method to concrete health factor is illustrated respectively below:
Select the plant material that mouth is light, look shallow of the integration of drinking and medicinal herbs of pure natural: puerarin, salvianolic acid B, trans-resveratrol, peoniflorin and Potenlini.
1) preparation of puerarin: the root of kudzu vine behind macroporous resin purification, is handled purifying, crystallization with the silica gel chromatography column chromatography through water extraction, promptly obtains white crystalline powder.
2) preparation of salvianolic acid B: red sage root water extraction, concentrate back adjust pH 2～3, use ethyl acetate extraction, be concentrated into no ethyl acetate flavor, thin up, adsorptive capacity is by macroporous resin, earlier with water elution in proportion, again with 20%～45% ethanol elution, collect elutriant, activated carbon decolorizing concentrates, enriched material finally obtains white crystalline powder handling purifying, crystallization through silica gel chromatographic column.
3) preparation of trans-resveratrol: when extracting trans-resveratrol, select 60%～80% ethanol to make extraction solvent, after extracting solution is concentrated into and is equivalent to the medicinal material charging capacity, add small diluted amount, diluent extracts with 10 times of volumes of acetic acid ethyl esters, extracting solution concentrate ethyl acetate extract, through the silica gel wash-out, collect elutriant, through decolouring, concentrate, freeze-drying promptly obtains white crystalline powder.
4) preparation of peoniflorin: when extracting peoniflorin, use water extraction, after extracting solution is concentrated into and is equivalent to the medicinal material charging capacity, add small diluted amount, diluent is by macroporous resin column, and first water is eluted to colourless, uses 30%～60% ethanol elution again, collect elutriant, through decolouring, concentrate, freeze-drying promptly obtains white crystalline powder.
5) preparation of Potenlini: when extracting Potenlini, select 60%～80% ethanol to make extraction solvent, after extracting solution is concentrated into and is equivalent to the medicinal material charging capacity, add small diluted amount, diluent is by macroporous resin column, and first water is eluted to colourless, uses 8 times of column volume 25%～50% ethanol elutions again, collect elutriant, through decolouring, concentrate, freeze-drying promptly obtains white crystalline powder.
6), make and protect liver health wine with a kind of above-mentioned white crystalline powder or severally dissolve in the 500ml liquor by prescription.
The quality of medicinal material standard that the present invention uses all meets " the Chinese people are state's officinal relevant regulations altogether ", and the physico-chemical property and the pharmacological effect that protect used medicinal material in the liver health wine are described below:
The root of kudzu vine is the integration of drinking and medicinal herbs article, and puerarin is a main effective constituent in the root of kudzu vine, and its molecular formula is 4, and 7-dihydroxyl-8-8-D-glucose isoflavones is white needle, water-soluble and ethanol.Modern medicine proof puerarin can by anti-oxidant, effectively remove oxyradical, alleviate approach such as hepatocellular infringement, the hepatocellular integrities of protection, the liver during to organophosphate poisoning shields.To crapulent patient, the root of kudzu vine have obviously alleviate dizziness, headache, feel sick, symptom such as vomiting, and can obviously improve liver function, reduce ALT and AST.So puerarin is to CCl
4Due to the rat acute liver injury have provide protection.Puerarin have toxicity little, be difficult in vivo savings, can be through advantages such as multipath drainages.Be present in Pericarpium Vitis viniferae, giant knotweed, the polyphenolic compound in the plants such as Chinese scholartree, have anti-inflammatory, anti-oxidative damage, protect effects such as liver and immunomodulatory.
Trans-resveratrol is a white powder, in water and ethanol certain dissolubility is arranged.Studies have shown that liver injury has significant protective effect to trans-resveratrol to Con A inductive, comparatively obvious with middle dosage (10mg/kg) group effect, show as that Serum ALT and AST actively reduce, the liver injury pathological change alleviates; Three dosage of trans-resveratrol big (20mg/kg), in the content of (10mg/kg), little (5mg/kg) group mice serum NO all significantly reduce, the active of heavy dose of, middle dosage group hepatic tissue SOD obviously raises, MDA content significantly reduces, with the model group comparing difference significance meaning is arranged, the liver mechanism of protecting of making veratryl alcohol clear and its anti peroxidation of lipid damage and to suppress the generation of NO closely related, thus theoretical basis provided for diseases such as trans-resveratrol treatment hepatitis B and immunity, liver injuries.
The red sage root contains various active compositions such as salviol, Salvianic acidA, TANSHINONES, and salviol, Salvianic acidA class are off-white color, good water solubility.The red sage root can alleviate hepatic cell fattydegeneration and the downright bad and inhibition TG content due to the alcohol.The red sage root is used for the control of alcoholic liver injury, the result shows, red sage root group and alcohol group are relatively, liver mitochondrion GSH level obviously raises and reaches 50%, Serum ALT obviously descends, light microscopic and Electronic Speculum show that all the popular name for reason obviously alleviates, and structures such as liver cell nuclear membrane, endoplasmic reticulum, plastosome are normal substantially.The prompting red sage root plays a good protection to alcoholic liver injury, and its mechanism of action is the generation that (1) red sage root can reduce free radical, suppresses the consumption of GSH, strengthens anti-oxidative defense ability in the body; (2) red sage root can be protected the liver plasma membrane system well, improves its stability.This experiment provides experimental evidence for the red sage root in the application of alcoholic liver disease aspect preventing and treating.
The Radix Paeoniae Alba total glucosides main component is a peoniflorin, and is soluble in water for white amorphous powder, can be dissolved in ethanol. Radix Paeoniae Alba total glucosides (TGP 10,20mg/kgd) prevention administration can obviously resist GalN or CCl
4Serum paddy third changes the rising of ammonia alcohol after the induced mice liver injury, and sero-abluminous decline and hepatic glycogen content reduce.And morphologic hepatocellular degeneration and necrosis are significantly improved and recover.Mitochondrial swelling in the liver cell, the vacuolar degeneration of entoplasm net, lysosomal coming off are recovered obviously also to illustrate that Radix Paeoniae Alba total glucosides is to CCl on the ultrastructure simultaneously
4The mouse liver oxidative damage that causes with ethanol has significant protective effect.
It is of the present invention that to protect the health factor that liver health wine added be that active ingredient of Chinese herbs is to have prevention and alleviate the activeconstituents of alcohol to the infringement of liver.This protects that liver health bartender has held that liquor is colourless, the characteristics of sweet-smelling, free from extraneous odour, simultaneously the mouse liver oxidative damage that ethanol causes is had significant protective effect.Solved the colony that custom is drunk for a long time, the health factor that can intangibly will have liver protection function when drinking is sent into intravital problem, reaches the purpose that effectively prevents or control alcoholic liver, fatty liver.Because this wine is colourless, sweet-smelling can also be as dinner party wine.
Drinking method: each cuvette, noon and evening respectively once also can increase the amount of drinking according to the capacity for liquor of oneself slightly, are as the criterion up to taking effect, and adhere to that the resistibility and the health of drinking increasing health are all helpful.
Through clinical experiment, the human body that protects liver health wine and have remarkable reduction ethanol to cause of the present invention is crossed the restraining effect of the acute rising of lipid, the SOD activity in the tissue that raises; Have obviously alleviate dizziness, headache, feel sick, symptom such as vomiting, and can obviously improve liver function, reduce ALT and AST.So experiment confirm is to CCl
4Due to the rat acute liver injury have provide protection.Health wine (TGP 10,20mg/kgd) prevention administration can obviously resist GalN or CCl
4Serum paddy third changes the rising of ammonia alcohol after the induced mice liver injury, and sero-abluminous decline and hepatic glycogen content reduce.And morphologic hepatocellular degeneration and necrosis are significantly improved and recover.Protect liver health wine and can alleviate hepatic cell fattydegeneration due to the alcohol and downright bad and suppress TG content.Health wine is used for the control of alcoholic liver injury, and the result shows, this product and the comparison of alcohol group, liver mitochondrion GSH level obviously raises and reaches 50%, Serum ALT obviously descends, and light microscopic and Electronic Speculum show that all the popular name for reason obviously alleviates, and structures such as liver cell nuclear membrane, endoplasmic reticulum, plastosome are normal substantially.
The present invention is on probation through people more than 100, and effect is obvious, and spirit is happy after drinking, obviously alleviate dizziness, headache, feel sick, symptom such as vomiting, and can obviously improve liver function, and for original Huang, after weak illness crowd takes, feel that generally health is comfortable.The liver health wine that protects that contains various compositions simultaneously shows through acute toxicity test and long term toxicity test: the health factor among the present invention comprises that one or more and the liquor compatibility in puerarin, trans-resveratrol, peoniflorin, Potenlini, the salvianolic acid B is without any side effects, safe and reliable.In different crowds, try out and obtained the extraordinary effect of drinking.
Embodiment 1
The preparation of health factor: highly purified puerarin, salvianolic acid B, trans-resveratrol, peoniflorin, Potenlini composition.
1. the preparation of puerarin: root of kudzu vine 500g boils 2 times with 14 times of decoctings, each 2 hours, filter, filtrate merges, and is evaporated to cream (relative density 1.10 clearly, 50 ℃), clear cream adds less water and is diluted to 2 times of crude drug amount, and last HPD100 macroporous resin is washed with 3 times of column volumes, water liquid discards, use 2 times of column volume 25% ethanol elutions again, use 4 times of column volume 70% ethanol elutions again, collect 70% ethanol eluate, concentrate, silica gel chromatographic column on the enriched material with ethyl acetate-methyl alcohol (15: 1) wash-out, is collected the puerarin composition, through concentrating, decolouring, crystallization treatment obtains white crystalline powder, and purity is higher than 90%.
2. the preparation of salvianolic acid B: red sage root 1000g is with 14 times of water extraction 2 times, extracting solution is concentrated into the clear cream that relative density is 1.05～1.12 (60 ℃), with dilute sulphuric acid adjust pH 2～3, divide 3 extractions with 4 times of volumes of acetic acid ethyl esters, extraction liquid is concentrated into the no ethyl acetate clear cream of distinguishing the flavor of, after adding 2 times of water dilutions, by the HPD100 macroporous resin, earlier with water elution, with 40% ethanol elution, collect elutriant again, activated carbon decolorizing, concentrated, enriched material finally obtains white crystalline powder through the silica gel chromatography column separating purification.
3. the preparation of trans-resveratrol: Pericarpium Vitis viniferae 5000g, select 80% ethanol to make extraction solvent for 50 liters, carry out diacolation, percolate is concentrated into does not have the clear cream of alcohol flavor, add 1.5 times water dilution, diluent extracts with 5 times of volumes of acetic acid ethyl esters, extracting solution concentrate ethyl acetate extract, through silica gel chromatographic column separate, purifying promptly obtains white crystalline powder.
4. the preparation of peoniflorin: root of herbaceous peony 1000g, with 14 times of water extraction 2 times, after extracting solution is concentrated into and is equivalent to the medicinal material charging capacity, add 95% ethanol to determining alcohol and reach 60%, left standstill 24 hours, supernatant liquor is evaporated to does not have the alcohol flavor, adds small diluted amount, by the HPD100 macroporous resin column, elder generation's water is eluted to colourless, use 60% ethanol elution again, collect elutriant, through decolouring, concentrate, enriched material crosses that silica gel chromatographic column separates, purifying promptly obtains white crystalline powder.
5. the preparation of Potenlini: Radix Glycyrrhizae 1000g, measure 5% ammoniacal liquor, 60% extraction using alcohols 2 times with 14 times, each 2 hours, after extracting solution is concentrated into and is equivalent to the medicinal material charging capacity, add 1 times of water gaging dilution, diluent is by the HPD100 macroporous resin column, elder generation's water is eluted to colourless, use 8 times of column volume 40% ethanol elutions again, collect elutriant, through decolouring, concentrate, freeze-drying promptly obtains white crystalline powder.
The described enforcement of following table is protected among the preparation method of liver health wine, and the number of degrees of liquor can not exert an influence to the injected volume of health factor.
| Sequence number | Liquor | Puerarin (mg) | Salvianolic acid (mg) | Potenlini (mg) | Trans-resveratrol (mg) | Peoniflorin (mg) |
| 1 | 500ml(38％) | 30 | 50 | 10 | 60 | |
| 2 | 500ml(38％) | 80 | ||||
| 3 | 500ml(45％) | 60 | ||||
| 4 | 500ml(52％) | 150 | 150 | |||
| 5 | 500ml(45％) | 50 | 60 | |||
| 6 | 500ml(38％) | 80 | ||||
| 7 | 500ml(57％) | 60 | 30 | 20 | ||
| 8 | 500ml(45％) | 50 | 20 | 70 | ||
| 9 | 500ml(57％) | 200 | ||||
| 10 | 500ml(45％) | 80 | 30 | 30 | 120 | |
| 11 | 500ml(45％) | 50 | ||||
| 12 | 500ml(38％) | 20 | 20 | 80 |
Take out fusion with the wine pump in the health factor input storage wine jar with above different proportionings, colourless through promptly can be made into behind the screen, clear saturating, free from extraneous odour has the sweet-smelling of wine, clear and definite, the stay-in-grade liver protecting health care of health factor wine.
It is worthy of note; protection scope of the present invention is not limited to above-mentioned specific examples mode; health factor can also be put into wine such as red wine, beer and yellow rice wine; all can realize the effect of identical liver protecting; according to basic fundamental design of the present invention; those of ordinary skills need not through creative work, and the embodiment that can associate all belongs to protection scope of the present invention.
Claims (5)
1. one kind protects the liver health wine, it is characterized in that: be made up of liquor and plant milk extract; Plant milk extract in the described liquor is puerarin, Potenlini, trans-resveratrol, peoniflorin, salvianolic acid B; The proportioning w/v of plant milk extract and liquor is 1mg～400mg/500ml; The liquor number of degrees are 0.5～65 degree.
2. the health wine that protects the liver according to claim 1 is characterized in that: plant milk extract puerarin 1～300mg, trans-resveratrol 1～200mg in the 500ml liquor, peoniflorin 1～396mg, Potenlini 1～200mg, salvianolic acid B 1～300mg.
3. the health wine that protects the liver according to claim 1 is characterized in that: plant milk extract puerarin 20～300mg, the trans-resveratrol 10～200mg of 500ml liquor, peoniflorin 20～330mg, Potenlini 20～200mg, salvianolic acid B 20～300mg.
4. the health wine that protects the liver according to claim 1 is characterized in that: plant milk extract puerarin 30mg, salvianolic acid B 50mg, Potenlini 50mg, trans-resveratrol 10mg, peoniflorin 60mg in 500ml 38% liquor.
5. one kind is used for preparing the described method that protects the liver health wine of the arbitrary claim of claim 2 to 4, it is characterized in that: comprise the steps:
(1) purification of plant milk extract
A. choose natural plant material: the root of kudzu vine, the red sage root, Pericarpium Vitis viniferae, the root of herbaceous peony and Radix Glycyrrhizae;
B. raw material is carried out boiling, extraction, alcohol precipitation, concentrates the acquisition concentrated solution;
C. concentrated solution is passed through macroporous resin purification, described macroporous resin purification comprises: upper prop, absorption and wash-out;
D. water and 10%～90% ethanol wash-out macroporous adsorbent resin is respectively successively collected elutriant, the dry crude product that obtains;
E. the purifying plant milk extract obtains the effective constituent compound monomer and protects the liver the plant milk extract white crystalline powder;
(2) protect the liver the finished product method of health wine
Plant milk extract puerarin, salvianolic acid B, trans-resveratrol, peoniflorin, Potenlini behind the above-mentioned E item gained purifying are put into storage wine jar in proportion, take out with wine by pump repeatedly and fully merge, behind molecular sieve filtration, obtain protecting the liver health wine.
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| CN101897746B (en) * | 2009-05-31 | 2012-11-07 | 中国科学院上海生命科学研究院 | Composite used for relieving alcoholic intoxication and protecting liver and preparation method and application thereof |
| CN103255022B (en) * | 2013-04-16 | 2014-04-30 | 国家林业局泡桐研究开发中心 | Eucommia ulmoides male flower wine and preparation method thereof |
| CN105326910A (en) * | 2015-10-28 | 2016-02-17 | 宋广林 | Health-preserving liquor and preparing method thereof |
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| CN109628265A (en) * | 2019-01-09 | 2019-04-16 | 贵州省中国科学院天然产物化学重点实验室(贵州医科大学天然产物化学重点实验室) | A kind of liver-protection health-care molecule wine and preparation method thereof containing general flavone |
| CN109833402B (en) * | 2019-04-01 | 2021-06-22 | 安徽农业大学 | Paeonia lactiflora pall anti-inflammatory liver-protecting wine and preparation method thereof |
| CN110184163A (en) * | 2019-07-12 | 2019-08-30 | 劲牌有限公司 | A kind of preparation method with the kudzuvine root wine for reducing hepatic injury function |
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|---|---|---|---|---|
| CN1448497A (en) * | 2002-03-28 | 2003-10-15 | 庄玉坤 | Alcoholic beverage capable of reducing hepatic injury or noninjuring liver |
| CN1472303A (en) * | 2003-07-11 | 2004-02-04 | 王德福 | Production of high content white veratrum alkaloids spirit |
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| Publication number | Publication date |
|---|---|
| CN101033445A (en) | 2007-09-12 |
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