CN107006433B - A kind of compound improving diamondback moth mating ratio and mating time - Google Patents
A kind of compound improving diamondback moth mating ratio and mating time Download PDFInfo
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- CN107006433B CN107006433B CN201710232041.7A CN201710232041A CN107006433B CN 107006433 B CN107006433 B CN 107006433B CN 201710232041 A CN201710232041 A CN 201710232041A CN 107006433 B CN107006433 B CN 107006433B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/04—Oxygen or sulfur attached to an aliphatic side-chain of a carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N49/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
Abstract
The present invention provides the compounds of a kind of raising diamondback moth mating ratio and mating time, are made of octanal, n-hexyl alcohol, geraniol, benzyl alcohol, cis- 3- hexenyl acetate, successively carry out proportion mixing according to 1 ~ 10:5 of mass ratio ~ 20:5 ~ 20:5 ~ 20:5 ~ 50.The compound is the volatile materials screened from a variety of host plants of diamondback moth, has the characteristics that function and effect are significant, harmless to polypide itself.The mating rate of the female moth of diamondback moth and male moth can be improved under the compound existence condition and extend its mating time, help to improve the egg laying amount of female moth, especially when using man-made feeds raising diamondback moth, the reproductive efficiency that the compound will significantly improve offspring is discharged in right amount, and good time guarantee is provided to research diamondback moth mating housing choice behavior diversity test.
Description
Technical field
The present invention relates to insect growth development and ecospheres, and in particular to a kind of raisings diamondback moth mate ratio with mate
The compound of time.
Background technique
Diamondback moth (Lepidoptera Lepidoptera: plutellidae；Lepidoptera:Plutellidae), it is commonly called as hang spring worm.
The worm has become the whole world and is distributed most wide lepidoptera pest, almost the countries and regions of all cultivation brassicaceous vegetables all
Have its record of causing harm (Sarfraz et al., 2005;Talekar and Shelton, 1993).The whole world is every year because of diamondback moth
Damage and yield loss and expenses for prevention and control summation are up to 40 ~ 5,000,000,000 dollars (Furlong et al., 2013).Diamondback moth is a kind of few food
Property pest, the main feeding brassicaceous vegetable blade of larva.
Populations of Diamondback Moth, Plutella Xylostella breeding is completed in laboratory, and there are mainly two types of method for breeding, first is that raised with host plant, second is that
Manually feed is raised.Under host plant raising, for every female adult pest egg laying amount at 180 or so, hatching rate is female 95.9%
Hero mating ratio 88.1%, but it is difficult to female moth egg amount, male and female mating rate statistical comparison, because female moth and male moth are hidden in
Under host's blade, its behavior can not be observed by photographic device；Under man-made feeds rearing conditions, every female adult pest egg laying amount exists
110 or so, 90% or so, male and female mate ratio 59.5% hatching rate, although facilitating the observation of egg laying amount statistics and behavior,
Be female moth and the survival of male moth time it is shorter, about 4-6 days, and man-made feeds breeding technology be not at present it is very mature, raising
There are also some differences under female, male mating behavior and host's raising existence condition under material raising, therefore we have developed a kind of raising
The compound of diamondback moth mating ratio and mating time, in the presence of the compound and man-made feeds, every female adult pest egg laying amount
At 170-180 or so, 97.5% or more, male and female mate 80.7% or more ratio hatching rate, the time-to-live of female moth and male moth
About 8-9 days, significantly improve female moth egg amount, male and female mating ratio and time-to-live.Project team is currently engaged in small
The research of diamond-back moth male and female mating housing choice behavior in the presence of host's volatile matter, and the content is not also appeared in the newspapers in document at home and abroad
Road, the development of compound of the present invention will study the diamondback moth housing choice behavior test that mates for project team and provide the time and ensure, also for
The mating behavior observation of other insects under man-made feeds raising provides reference and foundation.
Summary of the invention
The purpose of the present invention is to provide the compound of a kind of raising diamondback moth mating ratio and mating time, the compounds
It is the volatile materials screened from a variety of host plants of diamondback moth, has function and effect significant, harmless to polypide itself
The features such as.The mating rate of the female moth of diamondback moth and male moth can be improved under the compound existence condition and extend its mating time,
The egg laying amount of female moth is helped to improve, especially when using man-made feeds raising diamondback moth, discharging the compound in right amount will be shown
The reproductive efficiency for improving offspring is write, good time guarantee is provided to research diamondback moth mating housing choice behavior diversity test.
To achieve the above object, the present invention adopts the following technical scheme:
It is a kind of to improve diamondback moth mating ratio and the compound of mating time is using atoleine as solvent, by 50 μ g/ml
Octanal, n-hexyl alcohol, geraniol, benzyl alcohol, cis- 3- hexenyl acetate solution carry out 5 ~ 10:5 ~ 18:5 ~ 18:5 ~ 20:10 ~ 50
Carry out proportion mixing.
This 4 kinds of n-compounds of octanal, n-hexyl alcohol, geraniol, benzyl alcohol and cis- 3- hexenyl acetate recited above are equal
For host-brassicaceous vegetable volatile component of diamondback moth.
A kind of preparation method for the compound for improving diamondback moth mating ratio and mating time includes as described above for preparation
Following steps:
The preparation of the one-component solution of (1) 50 μ g/ml
The preparation of the octanal solution of 50 μ g/ml: 10 μ l are taken to add the octanal n-compound of pre-prepared 5 μ g/ μ l
Enter into the atoleine of 990 μ l, and uniformly mixes；
The preparation of the n-hexyl alcohol solution of 50 μ g/ml: the n-hexyl alcohol n-compound of pre-prepared 5 μ g/ μ l is taken into 10 μ
L is added in the atoleine of 990 μ l, and is uniformly mixed；
The preparation of the spiceleaf alcoholic solution of 50 μ g/ml: the geraniol n-compound of pre-prepared 5 μ g/ μ l is taken into 10 μ
L is added in the atoleine of 990 μ l, and is uniformly mixed；
The preparation of the benzyl alcohol solution of 50 μ g/ml: the benzyl alcohol n-compound of pre-prepared 5 μ g/ μ l is taken into 10 μ
L is added in the atoleine of 990 μ l, and is uniformly mixed；
The preparation of the cis- 3- hexenyl acetate solution of 50 μ g/ml: by the cis- 3- hexene second of pre-prepared 5 μ g/ μ l
Acid esters n-compound takes 10 μ l to be added in the atoleine of 990 μ l, and uniformly mixes；
(2) octanal for the 50 μ g/ml for obtaining step (1), n-hexyl alcohol, geraniol, benzyl alcohol, cis- 3- hexenyl acetate
5 ~ 10:5 of solution ~ 18:5 ~ 18:5 ~ 20:10 ~ 50 carry out proportion mixing, and compound is made.
Remarkable advantage of the invention is:
(1) efficient to polypide safety, effect
A kind of constituent of compound improving diamondback moth mating ratio and mating time produced by the present invention is cross
The flower main volatile ingredient of section vegetables, is native compound, harmless to insect itself；And it can be with during volatile matter release
Certain synergistic effect is played to insect sex pheromone, improves mating efficiency；
(2) application diversification
Compounds constituent components used in the present invention are host itself botanical components, be alternatively arranged as diamondback moth and its
The attractant component of its Cruciferous Vegetable Pests or its natural enemy is used.
(3) compound that the present invention develops can not only improve the mating ratio of female, male moth, moreover it is possible to extend its mating time.
In the presence of compound of the present invention, every female adult pest egg laying amount is at 170-180 or so, and hatching rate 97.5% or more, hand over by male and female
The time-to-live of 80.7% or more proportion, female moth and male moth is about 8-9 days, significantly improves female moth egg amount, male and female mating ratio
Rate and time-to-live.
Detailed description of the invention
Fig. 1 is that diamondback moth is female in the presence of the compound that the present invention develops, male mating ratio (average value+Standard error) signal
Figure；Different letters indicate significant difference (SSR, P < 0.05) on different colours in figure.
Fig. 2 is that diamondback moth is female in the presence of the compound that the present invention develops, male mating ratio (average value+Standard error) signal
Figure；* indicates significant difference (SSR, P < 0.05) in figure.
Fig. 3 is that diamondback moth is female in the presence of the compound that the present invention develops, male mating ratio (average value+Standard error) signal
Figure；* indicates significant difference (SSR, P < 0.05) in figure.
Specific embodiment
The present invention is further illustrated by the following examples, but protection scope of the present invention is not limited to following reality
Apply example.
Embodiment 1
One, a kind of preparation for the compound for improving diamondback moth mating ratio and mating time
The preparation of the one-component solution of 1.1 (1) 50 μ g/ml
The preparation of the octanal solution of 50 μ g/ml: 10 μ l are taken to add the octanal n-compound of pre-prepared 5 μ g/ μ l
Enter into the atoleine of 990 μ l, and uniformly mixes；
The preparation of the n-hexyl alcohol solution of 50 μ g/ml: the n-hexyl alcohol n-compound of pre-prepared 5 μ g/ μ l is taken into 10 μ
L is added in the atoleine of 990 μ l, and is uniformly mixed；
The preparation of the spiceleaf alcoholic solution of 50 μ g/ml: the geraniol n-compound of pre-prepared 5 μ g/ μ l is taken into 10 μ
L is added in the atoleine of 990 μ l, and is uniformly mixed；
The preparation of the benzyl alcohol solution of 50 μ g/ml: the benzyl alcohol n-compound of pre-prepared 5 μ g/ μ l is taken into 10 μ
L is added in the atoleine of 990 μ l, and is uniformly mixed；
The preparation of the cis- 3- hexenyl acetate solution of 50 μ g/ml: by the cis- 3- hexene second of pre-prepared 5 μ g/ μ l
Acid esters n-compound takes 10 μ l to be added in the atoleine of 990 μ l, and uniformly mixes；
A kind of 1.2 preparations for the compound for improving diamondback moth mating ratio and mating time
Octanal, n-hexyl alcohol, geraniol, benzyl alcohol, the cis- 3- hexenyl acetate solution for the 50 μ g/ml that step (1) is obtained
5:5: 5:5:10 proportion mixing is carried out, compound is made.
In the presence of compound of the present invention, every female adult pest egg laying amount is at 170 or so, and hatching rate is 97.5%, diamondback moth
Female, male mating ratio is as shown in Figure 3.
Embodiment 2
One, a kind of preparation for the compound for improving diamondback moth mating ratio and mating time
The preparation of the one-component solution of 1.1 (1) 50 μ g/ml
The preparation of the octanal solution of 50 μ g/ml: 10 μ l are taken to add the octanal n-compound of pre-prepared 5 μ g/ μ l
Enter into the atoleine of 990 μ l, and uniformly mixes；
The preparation of the n-hexyl alcohol solution of 50 μ g/ml: the n-hexyl alcohol n-compound of pre-prepared 5 μ g/ μ l is taken into 10 μ
L is added in the atoleine of 990 μ l, and is uniformly mixed；
The preparation of the spiceleaf alcoholic solution of 50 μ g/ml: the geraniol n-compound of pre-prepared 5 μ g/ μ l is taken into 10 μ
L is added in the atoleine of 990 μ l, and is uniformly mixed；
The preparation of the benzyl alcohol solution of 50 μ g/ml: the benzyl alcohol n-compound of pre-prepared 5 μ g/ μ l is taken into 10 μ
L is added in the atoleine of 990 μ l, and is uniformly mixed；
The preparation of the cis- 3- hexenyl acetate solution of 50 μ g/ml: by the cis- 3- hexene second of pre-prepared 5 μ g/ μ l
Acid esters n-compound takes 10 μ l to be added in the atoleine of 990 μ l, and uniformly mixes；
A kind of 1.2 preparations for the compound for improving diamondback moth mating ratio and mating time
Octanal, n-hexyl alcohol, geraniol, benzyl alcohol, the cis- 3- hexenyl acetate solution for the 50 μ g/ml that step (1) is obtained
8:10:10:15:30 proportion mixing is carried out, compound is made.
In the presence of compound of the present invention, every female adult pest egg laying amount is at 172 or so, and hatching rate is 97.8%, diamondback moth
Female, male mating ratio is as shown in Figure 2.
Embodiment 3
One, a kind of preparation for the compound for improving diamondback moth mating ratio and mating time
The preparation of the one-component solution of 1.1 (1) 50 μ g/ml
The preparation of the octanal solution of 50 μ g/ml: 10 μ l are taken to add the octanal n-compound of pre-prepared 5 μ g/ μ l
Enter into the atoleine of 990 μ l, and uniformly mixes；
The preparation of the n-hexyl alcohol solution of 50 μ g/ml: the n-hexyl alcohol n-compound of pre-prepared 5 μ g/ μ l is taken into 10 μ
L is added in the atoleine of 990 μ l, and is uniformly mixed；
The preparation of the spiceleaf alcoholic solution of 50 μ g/ml: the geraniol n-compound of pre-prepared 5 μ g/ μ l is taken into 10 μ
L is added in the atoleine of 990 μ l, and is uniformly mixed；
The preparation of the benzyl alcohol solution of 50 μ g/ml: the benzyl alcohol n-compound of pre-prepared 5 μ g/ μ l is taken into 10 μ
L is added in the atoleine of 990 μ l, and is uniformly mixed；
The preparation of the cis- 3- hexenyl acetate solution of 50 μ g/ml: by the cis- 3- hexene second of pre-prepared 5 μ g/ μ l
Acid esters n-compound takes 10 μ l to be added in the atoleine of 990 μ l, and uniformly mixes；
A kind of 1.2 preparations for the compound for improving diamondback moth mating ratio and mating time
Octanal, n-hexyl alcohol, geraniol, benzyl alcohol, the cis- 3- hexenyl acetate solution for the 50 μ g/ml that step (1) is obtained
10:18: 18:20:50 proportion mixing is carried out, compound is made.
In the presence of compound of the present invention, every female adult pest egg laying amount is at 180 or so, and hatching rate is 98.6%, diamondback moth
Female, male mating ratio is as shown in Figure 1.
The observation of diamondback moth in the presence of compound of the present invention of embodiment 4 female, male moth mating ratio and mating time
Using the mating ratio and mating time of infrared monitoring video recording system observation female, the male moth of diamondback moth, SUNHA
Digital IR CCD Camera infrared monitoring instrument is connect with computer.Infrared monitoring video recording system is set up in installation on computers.It will
Each 10 of female, the male moth just to have sprouted wings carries out pairing and is put into homemade Perspex case (10cm x 10cm x 15cm),
The compound of different proportion in the specific embodiment 3 of the invention developed is dripped in rubber tip respectively, hangs on organic glass sight
Box top middle position is examined, is placed under the camera of infrared monitoring instrument and carries out lasting monitoring video, is carried out continuously monitoring, directly
To diamondback moth is female, male moth is dead.Each experiment repeated observation 3 times.By the playback of infrared monitoring Video Document, can understand
And accurately record the specific time point of diamondback moth mating and female, the male moth ratio of each time point to mate.Control examination
It tests and does not place the compound.
As a result known to (as shown in Figure 1), the mating ratio maximum value of female, the male moth of (in the presence of no compound) diamondback moth is compareed
It is 320% ± 60%, in the presence of compound the 3rd day after emergence, the 3rd day mating ratio is 390% ± 65%, is significantly higher than pair
According to.The present invention develop compound in the presence of, the 3rd day after emergence, 4 days, 5 days, 6 days, 7 days, 8 days mating ratios it is significantly high
In control.Diamondback moth mating ratio is that the 0, the 6th day female, male moth is substantially dead after the 5th day in control；With the presence of compound
Under, diamondback moth still has certain mating ratio on the 8th day after sprouting wings, just dead to the 9th day female, male moth.Illustrate development of the present invention
Compound in the presence of can extend diamondback moth mating time.
It is described above to be only used as embodiments of the present invention and example, without departing from this in this professional skill field range
Under the premise of inventive principle, equivalent transformation and improvement are carried out to it, are accordingly to be regarded as protection scope of the present invention.
Claims (4)
1. it is a kind of improve diamondback moth mating ratio and mating time compound, it is characterised in that: the compound by octanal,
N-hexyl alcohol, geraniol, benzyl alcohol, cis- 3- hexenyl acetate composition, successively according to 1 ~ 10:5 of mass ratio ~ 20:5 ~ 20:5 ~ 20:5
~ 50 carry out proportion mixing.
2. a kind of compound for improving diamondback moth mating ratio and mating time according to claim 1, feature exist
In: the compound is made of octanal, n-hexyl alcohol, geraniol, benzyl alcohol, cis- 3- hexenyl acetate, successively according to mass ratio
8:10:10:15:30 carries out proportion mixing.
3. a kind of side for preparing a kind of compound for improving diamondback moth mating ratio and mating time as described in claim 1
Method, it is characterised in that: it carries out preparing one-component solution using atoleine as solvent, is configured to 50 μ g/ml, it then will be pungent
Aldehyde, n-hexyl alcohol, geraniol, benzyl alcohol, cis- 3- hexenyl acetate according to 1 ~ 10:5 of mass ratio ~ 20:5 ~ 20:5 ~ 20:5 ~ 50 into
Row proportion mixing, is made compound.
4. a kind of preparation method of compound for improving diamondback moth mating ratio and mating time according to claim 3,
It is characterized by: specific step is as follows:
The preparation of the one-component solution of (1) 50 μ g/ml
The preparation of the octanal solution of 50 μ g/ml: 10 μ l are taken to be added to the octanal n-compound of pre-prepared 5 μ g/ μ l
In the atoleine of 990 μ l, and uniformly mix；
The preparation of the n-hexyl alcohol solution of 50 μ g/ml: 10 μ l are taken to add the n-hexyl alcohol n-compound of pre-prepared 5 μ g/ μ l
Enter into the atoleine of 990 μ l, and uniformly mixes；
The preparation of the spiceleaf alcoholic solution of 50 μ g/ml: 10 μ l are taken to add the geraniol n-compound of pre-prepared 5 μ g/ μ l
Enter into the atoleine of 990 μ l, and uniformly mixes；
The preparation of the benzyl alcohol solution of 50 μ g/ml: 10 μ l are taken to add the benzyl alcohol n-compound of pre-prepared 5 μ g/ μ l
Enter into the atoleine of 990 μ l, and uniformly mixes；
The preparation of the cis- 3- hexenyl acetate solution of 50 μ g/ml: by the cis- 3- hexenyl acetate of pre-prepared 5 μ g/ μ l
N-compound takes 10 μ l to be added in the atoleine of 990 μ l, and uniformly mixes；
(2) octanal for the 50 μ g/ml for obtaining step (1), n-hexyl alcohol, geraniol, benzyl alcohol, cis- 3- hexenyl acetate solution
It mixes according to the ratio, compound is made.
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| CN101347132B (en) * | 2008-09-02 | 2010-09-15 | 广东省昆虫研究所 | Attractant for diamondback moth |
| JP6467177B2 (en) * | 2013-09-30 | 2019-02-06 | 国立研究開発法人農業・食品産業技術総合研究機構 | Natural enemy insect attractant component derived from floral scent and its use |
| CN104094928B (en) * | 2014-07-31 | 2016-01-06 | 福建省农业科学院植物保护研究所 | Leaf weevil attractant and preparation method thereof cut by a kind of mango |
| CN104322531B (en) * | 2014-10-22 | 2017-02-22 | 深圳百乐宝生物农业科技有限公司 | Plutella xylostella attractant |
| CN105123718A (en) * | 2015-08-21 | 2015-12-09 | 中国农业科学院植物保护研究所 | Reverse sex pheromone for prevention and control of plutella xylostella l. |
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