CN106987551A - Gene-amplificative instrament and control method - Google Patents
Gene-amplificative instrament and control method Download PDFInfo
- Publication number
- CN106987551A CN106987551A CN201710136646.6A CN201710136646A CN106987551A CN 106987551 A CN106987551 A CN 106987551A CN 201710136646 A CN201710136646 A CN 201710136646A CN 106987551 A CN106987551 A CN 106987551A
- Authority
- CN
- China
- Prior art keywords
- controller
- air
- electromagnet
- gene amplification
- time period
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000012360 testing method Methods 0.000 claims abstract description 47
- 238000010438 heat treatment Methods 0.000 claims abstract description 34
- 238000001514 detection method Methods 0.000 claims abstract description 21
- 230000004544 DNA amplification Effects 0.000 claims description 29
- 230000001427 coherent effect Effects 0.000 claims description 26
- 238000003860 storage Methods 0.000 claims description 19
- 230000009471 action Effects 0.000 claims description 12
- 238000002474 experimental method Methods 0.000 claims description 8
- 230000005611 electricity Effects 0.000 claims description 6
- 230000036982 action potential Effects 0.000 claims description 3
- 230000003028 elevating effect Effects 0.000 claims description 3
- 230000005284 excitation Effects 0.000 claims description 3
- 230000001965 increasing effect Effects 0.000 claims description 3
- 238000011084 recovery Methods 0.000 claims description 3
- 230000000284 resting effect Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 6
- 108090000623 proteins and genes Proteins 0.000 abstract description 6
- 238000003825 pressing Methods 0.000 description 10
- 230000007246 mechanism Effects 0.000 description 6
- 210000003205 muscle Anatomy 0.000 description 4
- 241000973497 Siphonognathus argyrophanes Species 0.000 description 2
- 230000001914 calming effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000004378 air conditioning Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of gene-amplificative instrament and control method, including the controller on casing, heat lid, lowering or hoisting gear and the tumbler being connected with lowering or hoisting gear upper end；Heat lid is connected with tumbler, several vertical slots corresponding with each test tube jack in gene magnification module are provided with heat lid, air bag is equipped with each vertical slot, air bag lower end is provided with cover plate, each air bag is connected by airway tube with air accumulator, pressure sensor is provided with air accumulator, air accumulator is provided with air pump, magnetic valve is equipped with each airway tube, be provided with heating film in heat lid, controller respectively with air pump, heater, heating film, lowering or hoisting gear, tumbler, calm the anger sensor and each magnetic valve is electrically connected.The characteristics of present invention has good sealing effect, detection accuracy is high.
Description
Technical Field
The invention relates to the technical field of gene amplification equipment, in particular to a gene amplification instrument with good sealing effect and high detection accuracy and a control method.
Background
The gene amplification instrument is an indispensable instrument in the fields of biological scientific research, gene replication and the like, and is also a necessary instrument for the research in the field of biological genetic engineering. When the gene expansion instrument is used, a gene solution to be amplified is added into a test tube, then related reagents are added into the test tube, the test tube is placed on a heating module in the gene expansion instrument to heat the test tube, and the gene to be tested in the test tube is rapidly amplified through temperature change, so that the gene amplification test requirement is met.
When doing the experiment of gene amplification instrument, must cover the upper cover of gene amplification instrument, through adjusting the hot lid of test tube mouth upper end, compress tightly and strictly sealed the test tube mouth, avoid the liquid in the test tube to lead to the test result to appear the deviation owing to being heated the evaporation.
Chinese patent grant publication no: CN201080478Y, Announcement date 2008, 07.02.9.A "hot cover pressing device for gene amplification instrument" comprises a pressing mechanism, a hot cover, a slide way and an adjusting knob, and is characterized in that a screw rod capable of lifting the pressing mechanism is arranged between the pressing mechanism and the adjusting knob, four connecting shafts between the pressing mechanism and the hot cover are respectively provided with homogeneous springs, the periphery of the pressing mechanism is provided with symmetrically distributed balance rollers, and the balance rollers are matched with the slide way to form sliding fit connection. The shortcomings are that the hot cover pressing device of the gene amplification instrument only has a simple lifting function, the pressure of the hot cover on the test tube cannot be accurately mastered in the lifting process, the pressing strength of the hot cover and the test tube can be adjusted only by the experience of an operator, and for the test tubes with different heights or different strengths, the operator needs to lift the pressing mechanism by a certain amount, so that the strength of crushing the test tube or pressing the test tube due to improper operation is easily caused by the experience of the operator.
Disclosure of Invention
The invention aims to overcome the defects of poor sealing effect and low detection precision in the prior art and provides a gene amplification instrument with good sealing effect and high detection precision and a control method.
In order to achieve the purpose, the invention adopts the following technical scheme:
a gene amplification instrument comprises a box body, a gene amplification module and a heating device which are arranged in the box body, and also comprises a controller, a hot cover, a lifting device and a rotating device which is connected with the upper end of the lifting device, wherein the controller, the hot cover, the lifting device and the rotating device are arranged on the box body; the hot lid is connected with rotating device, be equipped with the corresponding perpendicular groove of each test tube jack in a plurality of and the gene amplification module in the hot lid, all be equipped with the gasbag in every perpendicular groove, the gasbag lower extreme is equipped with the apron, each gasbag all passes through air duct and gas tank connection, be equipped with pressure sensor in the gas holder, be equipped with the air pump on the gas holder, all be equipped with the solenoid valve on every air duct, be equipped with the heating film in the hot lid, the controller respectively with the air pump, heating device, the heating film, elevating gear, rotating device, sensor and each solenoid valve electricity of calming anger are connected.
The invention utilizes the air pressure of the air storage tank to uniformly control the pressure of the air bag on each cover plate, thereby ensuring that the pressure between test tubes with different heights and the corresponding cover plates is kept consistent, and meeting the requirement of the diversity of the test tube models used in experiments.
Preferably, a cylindrical first electromagnet is arranged in each vertical groove, an annular second electromagnet is arranged on the side face of each cover plate, and each first electromagnet and each second electromagnet are electrically connected with the controller. The first electromagnet and the second electromagnet are used for fixing the position of the cover plate, and the cover plate is prevented from being displaced due to unstable air pressure of the air bag.
As preferred, the diameter of the cross section of each perpendicular groove has a plurality of unidimensional, all is equipped with a plurality of direction on every perpendicular groove and erects the muscle, all is equipped with on the apron of each perpendicular groove and erects the corresponding breach of muscle with each direction. Each vertical guide rib provides a guide function for the movement of the cover plate.
Preferably, the lifting device comprises a fixed plate arranged on the side wall of the box body, a limit sensor arranged on the fixed plate, two vertical slide rails and a fixed frame; fixed frame and two vertical slide rail sliding connection, elevator motor are connected with fixed frame through the pivot that is equipped with the external screw thread, and limit sensor and elevator motor all are connected with the controller electricity.
The limiting sensor is used for sensing the descending height of the fixed frame, and when the limiting sensor detects the fixed frame in the descending process of the fixed frame, the controller controls the lifting motor to stop working.
Preferably, the rotating device comprises a shaft support frame arranged at the upper part of the fixed frame, a rotating shaft arranged on the shaft support frame, a rotating motor connected with the rotating shaft, and a rotating arm connected with the rotating shaft, wherein the rotating arm is connected with the hot cover, and the rotating motor is electrically connected with the controller.
A control method of a gene amplification instrument comprises the following steps:
(6-1) respectively placing a plurality of test tubes for experiments into each test tube jack by a worker, controlling the rotating device to drive the hot cover to rotate to a horizontal position by the controller, and controlling the lifting device to drive the hot cover to descend to a preset position by the controller;
(6-2) detecting the pressure in the gas storage tank by the pressure sensor, controlling the air pump to work by the controller, gradually increasing the air pressure in the gas storage tank, and keeping the air pressure in the gas storage tank within a preset pressure range [ A1, A2] in the controller after T1 minutes;
for the vertical groove with the test tube, the controller controls the electromagnetic valve of the air guide tube connected with the air bag in the vertical groove to be opened, and the air bag drives each cover plate to move downwards;
(6-3)
(6-3-1) the controller controls the air pressure of the air storage tank to be maintained within a pressure range [ A1, A2 ];
(6-3-2) the controller controls the first electromagnet and the corresponding second electromagnet which are provided with the vertical groove of the test tube to be electrified, the first electromagnet and the second electromagnet attract each other under the action of magnetic force, and the cover plate is kept stable;
(6-4) the controller controls the heating device and the heating film to heat each test tube, and after the preset heating time in the controller is reached, the controller controls the air pump to work to gradually reduce the air pressure in the air storage tank; the controller controls each first electromagnet and each second electromagnet to be powered off; the controller controls the heating device and the heating film to stop heating;
(6-5) the controller controls the lifting device to drive the hot cover to ascend to a preset position, the controller controls the rotating device to drive the hot cover to rotate, the hot cover is opened, and the staff take out each test tube.
Preferably, the following steps are included between the step (6-3-1) and the step (6-3-2):
(7-1) selecting detection values of the front and rear pressure sensors in a time period with the length of L by the controller; the two time periods are respectively a time period A and a time period B, and if L is n multiplied by TT, the n detection values of the pressure sensors in the time period A and the time period B are obtained through control;
(7-2) setting each detection value Sc of the time period A to xiEach detection value Sc of the period B is yi，i＝1，2，...，n；
Using formulasCalculating the similarity of Sc corresponding to the two time periods;
(7-3) if siIf < 1, will be compared with siCorresponding to yiDeleting; wherein,is the average of all Sc over the time period a,is the average of all Sc over time period B;
(7-4) the controller utilizes the remaining y during the time period BiThe detection signal I (t) of the pressure sensor is formed;
(7-5) inputting I (t) into the coherent resonance model, and adjusting the mu value of the coherent resonance model to enable the coherent resonance model to resonate;
(7-6) outputting the cross correlation coefficient by the coherent resonance model, and if the cross correlation coefficient is in the interval [0.85, 1.1], switching to the step (6-3-2); otherwise, the step (6-3-1) is carried out.
The judgment of whether the cross correlation coefficient is in the set interval can fix the position of the cover plate in the interval of meeting the pressure requirement of the gas storage tank, thereby meeting the requirement of sealing property, ensuring that the test tube is not damaged, improving the detection accuracy, improving the success rate of the experiment and reducing the production cost.
Preferably, the coherent resonance model is
Wherein V (t) is the action potential of the model trigger unit, VTIs a model trigger action threshold potential, VRIs the recovery potential after the trigger unit action is completed, mu tau is the resting state parameter after the model trigger action, VR＜VTξ (t) Gaussian random excitation parameter, V (t) is the real-time potential of the coherent resonance model, μ is the adjustment coefficient of the coherent resonance model, τ is the rest constant of the coherent resonance model, and V (t)+) Is a coherent resonance model at t+Real-time potential of time, V2(t) is the square of V (t), μ2τ is μ2The product of τ.
Preferably, the edge of the lower surface of each cover plate is provided with a coaming perpendicular to the cover plate, an annular vertical groove is arranged in each coaming, and a sealing ring is arranged in each annular vertical groove.
The arrangement of the coaming, the annular vertical groove and the sealing ring ensures that the sealing effect of the cover plate is better.
Therefore, the invention has the following beneficial effects:
the pressure of each air bag and the corresponding cover plate is uniformly controlled by utilizing the air pressure of the air storage tank, so that the pressure between test tubes with different heights and the corresponding cover plates is kept consistent, and the requirement on the diversity of the test tube models used in experiments is met;
the sealing effect is good, the test tube is not easy to be damaged, the detection accuracy is improved, the experiment success rate is improved, and the production cost is reduced.
FIG. 1 is a schematic diagram of an embodiment of the present invention;
FIG. 2 is a cross-sectional view of the thermal cover of the present invention;
FIG. 3 is a top view of the thermal cover of the present invention;
FIG. 4 is a functional block diagram of the present invention;
fig. 5 is a flow chart of the present invention.
In the figure: the air-conditioning system comprises a box body 1, a controller 2, a lifting device 3, a rotating device 4, a vertical groove 5, an air bag 6, a cover plate 7, an air storage tank 8, an air guide pipe 9, a first electromagnet 10, a second electromagnet 11, a hot cover 12, a fixing plate 31, a limit sensor 32, a fixing frame 33, a lifting motor 34, a shaft supporting frame 41, a rotating shaft 42, a rotating motor 43, a rotating arm 44, a pressure sensor 81, an air pump 82 and an electromagnetic valve 91.
Detailed Description
The invention is further described with reference to the following figures and detailed description.
The gene amplification instrument shown in fig. 1 comprises a box body 1, a gene amplification module and a heating device which are arranged in the box body, and is characterized by further comprising a controller 2, a heat cover 12, a lifting device 3 and a rotating device 4 which is connected with the upper end of the lifting device and is arranged on the box body; the hot lid is connected with rotating device, as shown in fig. 2, fig. 3, be equipped with 8 in the hot lid and the gene amplification module each test tube jack corresponding perpendicular groove 5, all be equipped with gasbag 6 in every perpendicular groove, the gasbag lower extreme is equipped with apron 7, each gasbag all is connected with gas holder 8 through air duct 9, as shown in fig. 4, be equipped with pressure sensor 81 in the gas holder, be equipped with air pump 82 on the gas holder, all be equipped with solenoid valve 91 on every air duct, be equipped with the heating film in the hot lid, the controller respectively with the air pump, heating device, the heating film, elevating gear, rotating device, the sensor of calming anger and each solenoid valve electricity are connected.
A cylindrical first electromagnet 10 is arranged in each vertical groove, an annular second electromagnet 11 is arranged on the side face of each cover plate, and each first electromagnet and each second electromagnet are electrically connected with a controller. The lower surface edge of each cover plate is provided with a coaming perpendicular to the cover plate, an annular vertical groove is arranged in each coaming, and a sealing ring is arranged in each annular vertical groove.
The diameter of each perpendicular cross section in groove has 2 unidimensional, all is equipped with a plurality of direction on every perpendicular groove and erects the muscle, all is equipped with on the apron in each perpendicular groove and erects the corresponding breach of muscle with each direction.
As shown in fig. 1, the lifting device includes a fixed plate 31 disposed on the sidewall of the box body, a limit sensor 32 disposed on the fixed plate, two vertical slide rails, and a fixed frame 33; fixed frame and two vertical slide rail sliding connection, elevator motor 34 is connected with fixed frame through the pivot that is equipped with the external screw thread, and limit sensor and elevator motor all are connected with the controller electricity.
The rotating device comprises a shaft support frame 41 arranged on the upper part of the fixed frame, a rotating shaft 42 arranged on the shaft support frame, a rotating motor 43 connected with the rotating shaft, and a rotating arm 44 connected with the rotating shaft, wherein the rotating arm is connected with the hot cover, and the rotating motor is electrically connected with the controller.
As shown in FIG. 5, a method for controlling a gene amplification apparatus includes the steps of:
step 100, installing a test tube and covering a hot cover
The working personnel respectively place a plurality of test tubes for experiments into each test tube jack, the controller controls the rotating device to drive the hot cover to rotate to the horizontal position, and the controller controls the lifting device to drive the hot cover to descend to the preset position;
step 200, inflating each air bag, and enabling each cover plate to be in contact with each test tube port
The pressure sensor detects the pressure in the gas storage tank, the controller controls the air pump to work, the air pressure in the gas storage tank is gradually increased, and after 5 minutes, the air pressure in the gas storage tank is always in a preset pressure range [ A1, A2] in the controller;
for the vertical groove with the test tube, the controller controls the electromagnetic valve of the air guide tube connected with the air bag in the vertical groove to be opened, and the air bag drives each cover plate to move downwards;
step 300, selecting a suitable interval to fix the cover plate
Step 310, the controller controls the air pressure of the air storage tank to be maintained within a pressure range [ A1, A2 ];
(7-1) selecting detection values of the front and rear pressure sensors in a time period with the length of L by the controller; the two preceding and succeeding time periods are respectively a time period A and a time period B, and if L is n multiplied by TT, n detection values of the pressure sensors in the time period A and the time period B are obtained through control, wherein n is 60, L is 1 minute, and LL is 1 second;
(7-2) setting each detection value Sc of the time period A to xiEach detection value Sc of the period B is yi，i＝1，2，...，n；
Using formulasCalculating the similarity of Sc corresponding to the two time periods;
(7-3) if siIf < 1, will be compared with siCorresponding to yiDeleting; wherein,is the average of all Sc over the time period a,is the average of all Sc over time period B;
(7-4) the controller utilizes the remaining y during the time period BiThe detection signal I (t) of the pressure sensor is formed;
(7-5) inputting I (t) into the coherent resonance model, and adjusting the mu value of the coherent resonance model to enable the coherent resonance model to resonate;
wherein V (t) is the action potential of the model trigger unit, VTIs a model trigger action threshold potential, VRIs the recovery potential after the trigger unit action is completed, mu tau is the resting state parameter after the model trigger action, VR＜VTξ (t) Gaussian random excitation parameter, V (t) is the real-time potential of the coherent resonance model, μ is the adjustment coefficient of the coherent resonance model, τ is the rest constant of the coherent resonance model, and V (t)+) Is a coherent resonance model at t+Real-time potential of time, V2(t) is the square of V (t), μ2τ is μ2The product of τ;
(7-6) outputting the cross correlation coefficient by the coherent resonance model, and if the cross correlation coefficient is in the interval [0.85, 1.1], turning to the step 320; otherwise, go to step 310.
320, controlling the first electromagnet and the corresponding second electromagnet of the vertical groove with the test tube to be electrified by the controller, wherein the first electromagnet and the second electromagnet attract each other under the action of magnetic force, and the cover plate is kept stable;
step 400, heating the test tube
The controller controls the heating device and the heating film to heat each test tube, and after the preset heating time in the controller is reached, the controller controls the air pump to work to gradually reduce the air pressure in the air storage tank; the controller controls each first electromagnet and each second electromagnet to be powered off; the controller controls the heating device and the heating film to stop heating;
step 500, taking out the test tube
The controller controls the lifting device to drive the hot cover to ascend to a preset position, the controller controls the rotating device to drive the hot cover to rotate, the hot cover is opened, and the workers take out the test tubes.
It should be understood that this example is for illustrative purposes only and is not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.
Claims (9)
1. A gene amplification instrument comprises a box body (1), a gene amplification module and a heating device which are arranged in the box body, and is characterized by also comprising a controller (2) arranged on the box body, a hot cover (412), a lifting device (3) and a rotating device (4) connected with the upper end of the lifting device; the hot lid is connected with rotating device, be equipped with corresponding perpendicular groove (5) of each test tube jack in a plurality of and the gene amplification module in the hot lid, all be equipped with gasbag (6) in every perpendicular groove, the gasbag lower extreme is equipped with apron (7), each gasbag all is connected with gas holder (8) through air duct (9), be equipped with pressure sensor (81) in the gas holder, be equipped with air pump (82) on the gas holder, all be equipped with solenoid valve (91) on every air duct, be equipped with the heating film in the hot lid, the controller respectively with the air pump, heating device, the heating film, elevating gear, rotating device, sensor and each solenoid valve electricity compressed air are connected.
2. The gene amplification apparatus according to claim 1, wherein a first cylindrical electromagnet (10) is provided in each vertical groove, a second annular electromagnet (11) is provided on a side surface of each cover plate, and each first electromagnet and each second electromagnet are electrically connected to the controller.
3. The gene amplification apparatus of claim 1, wherein the diameter of the cross section of each vertical groove has a plurality of different sizes, each vertical groove has a plurality of guide vertical ribs, and the cover plate of each vertical groove has notches corresponding to the guide vertical ribs.
4. The gene amplification instrument of claim 1, wherein the lifting device comprises a fixing plate (31) arranged on the side wall of the box body, a limit sensor (32) arranged on the fixing plate, two vertical slide rails and a fixing frame (33); fixed frame and two vertical slide rail sliding connection, elevator motor (34) are connected with fixed frame through the pivot that is equipped with the external screw thread, and limit sensor and elevator motor all are connected with the controller electricity.
5. The gene amplification apparatus according to claim 4, wherein the rotation means comprises a shaft support (41) provided at an upper portion of the fixed frame, a rotation shaft (42) provided at the shaft support, a rotation motor (43) connected to the rotation shaft, and a rotation arm (44) connected to the rotation shaft, the rotation arm being connected to the heat cover, the rotation motor being electrically connected to the controller.
6. A method for controlling a gene amplification apparatus according to claim 2, comprising the steps of:
(6-1) respectively placing a plurality of test tubes for experiments into each test tube jack by a worker, controlling the rotating device to drive the hot cover to rotate to a horizontal position by the controller, and controlling the lifting device to drive the hot cover to descend to a preset position by the controller;
(6-2) detecting the pressure in the gas storage tank by the pressure sensor, controlling the air pump to work by the controller, gradually increasing the air pressure in the gas storage tank, and keeping the air pressure in the gas storage tank within a preset pressure range [ A1, A2] in the controller after T1 minutes;
for the vertical groove with the test tube, the controller controls the electromagnetic valve of the air guide tube connected with the air bag in the vertical groove to be opened, and the air bag drives each cover plate to move downwards;
(6-3)
(6-3-1) the controller controls the air pressure of the air storage tank to be maintained within a pressure range [ A1, A2 ];
(6-3-2) the controller controls the first electromagnet and the corresponding second electromagnet which are provided with the vertical groove of the test tube to be electrified, the first electromagnet and the second electromagnet attract each other under the action of magnetic force, and the cover plate is kept stable;
(6-4) the controller controls the heating device and the heating film to heat each test tube, and after the preset heating time in the controller is reached, the controller controls the air pump to work to gradually reduce the air pressure in the air storage tank; the controller controls each first electromagnet and each second electromagnet to be powered off; the controller controls the heating device and the heating film to stop heating;
(6-5) the controller controls the lifting device to drive the hot cover to ascend to a preset position, the controller controls the rotating device to drive the hot cover to rotate, the hot cover is opened, and the staff take out each test tube.
7. The method for controlling a gene amplification apparatus according to claim 6, wherein the following steps are included between the step (6-3-1) and the step (6-3-2):
(7-1) selecting detection values of the front and rear pressure sensors in a time period with the length of L by the controller; the two time periods are respectively a time period A and a time period B, and if L is n multiplied by TT, the n detection values of the pressure sensors in the time period A and the time period B are obtained through control;
(7-2) setting each detection value Sc of the time period A to xiEach detection value Sc of the period B is yi，i＝1，2，...，n；
Using formulasCalculating the similarity of Sc corresponding to the two time periods;
(7-3) if siIf < 1, will be compared with siCorresponding to yiDeleting; wherein,is the average of all Sc over the time period a,is the average of all Sc over time period B;
(7-4) the controller utilizes the remaining y during the time period BiThe detection signal I (t) of the pressure sensor is formed;
(7-5) inputting I (t) into the coherent resonance model, and adjusting the mu value of the coherent resonance model to enable the coherent resonance model to resonate;
(7-6) outputting the cross correlation coefficient by the coherent resonance model, and if the cross correlation coefficient is in the interval [0.85, 1.1], switching to the step (6-3-2); otherwise, the step (6-3-1) is carried out.
8. The method for controlling a gene amplification apparatus according to claim 7, wherein the coherent resonance model is
Wherein V (t) is the action potential of the model trigger unit, VTIs a model trigger action threshold potential, VRIs the recovery potential after the trigger unit action is completed, mu tau is the resting state parameter after the model trigger action, VR＜VTξ (t) Gaussian random excitation parameter, V (t) is the real-time potential of the coherent resonance model, μ is the adjustment coefficient of the coherent resonance model, τ is the rest constant of the coherent resonance model, and V (t)+) Is a coherent resonance model at t+Real-time potential of time, V2(t) is the square of V (t), μ2τ is μ2The product of τ.
9. The method for controlling a gene amplification apparatus according to claim 7 or 8, wherein a skirt is provided on the lower surface of each cover plate so as to be perpendicular to the cover plate, an annular vertical groove is provided in the skirt, and a seal ring is provided in the annular vertical groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710136646.6A CN106987551B (en) | 2017-03-08 | 2017-03-08 | Gene amplification instrument and control method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710136646.6A CN106987551B (en) | 2017-03-08 | 2017-03-08 | Gene amplification instrument and control method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106987551A true CN106987551A (en) | 2017-07-28 |
| CN106987551B CN106987551B (en) | 2020-07-17 |
Family
ID=59411659
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710136646.6A Active CN106987551B (en) | 2017-03-08 | 2017-03-08 | Gene amplification instrument and control method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106987551B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004106922A1 (en) * | 2003-05-30 | 2004-12-09 | Instituto Nacional De Tecnica Aeroespacial 'esteban Terradas' | Method and apparatus for the detection of substances or analytes from the analysis of one or more samples |
| CN103725602A (en) * | 2012-10-16 | 2014-04-16 | 常州福生生物技术有限公司 | Thermal cover plate used in nucleic acid amplification detector |
| CN205562538U (en) * | 2016-05-04 | 2016-09-07 | 三峡大学 | A heating temperature regulating device for ground mechanical test |
| CN106459871A (en) * | 2014-05-21 | 2017-02-22 | 卡尤迪生物科技（北京）有限公司 | Thermal cycler lid configuration and use thereof |
-
2017
- 2017-03-08 CN CN201710136646.6A patent/CN106987551B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004106922A1 (en) * | 2003-05-30 | 2004-12-09 | Instituto Nacional De Tecnica Aeroespacial 'esteban Terradas' | Method and apparatus for the detection of substances or analytes from the analysis of one or more samples |
| CN103725602A (en) * | 2012-10-16 | 2014-04-16 | 常州福生生物技术有限公司 | Thermal cover plate used in nucleic acid amplification detector |
| CN106459871A (en) * | 2014-05-21 | 2017-02-22 | 卡尤迪生物科技（北京）有限公司 | Thermal cycler lid configuration and use thereof |
| CN205562538U (en) * | 2016-05-04 | 2016-09-07 | 三峡大学 | A heating temperature regulating device for ground mechanical test |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106987551B (en) | 2020-07-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106987522B (en) | The hot lid arrangement and control method of gene-amplificative instrament | |
| CN105259016A (en) | Full-automatic digestion instrument | |
| CN106987551B (en) | Gene amplification instrument and control method | |
| JPH08290218A (en) | Bending device and bending method | |
| CN110646631A (en) | Calibration liquid, sample liquid conveying and heating device and POCT detection equipment | |
| CN214334874U (en) | Smoke detector calibration device | |
| CN206696140U (en) | A kind of textile, which drips, to spread and the test integrated test equipment of moisture evaporation | |
| CN105033428B (en) | A kind of welding equipment | |
| CN106932548A (en) | The total oxygen analyzer of finished beer and its assay method | |
| CN201269800Y (en) | Constant temperature extruding experiment machine for battery | |
| CN114414177B (en) | Detection device and system for centrifugal plant hollow capsule production | |
| US4573502A (en) | Container filling apparatus and method | |
| CN101251948B (en) | Detecting system and method for detecting temperature type alarm | |
| CN202442937U (en) | Hanging drop control device for measuring oil-gas interfacial tension | |
| CN102564908B (en) | Hanging drop control method and device for measuring oil-gas interface tension | |
| CN205138877U (en) | Full -automatic appearance of clearing up | |
| CN208171533U (en) | Deep-well plates apparatus for testing weeping | |
| JP7334421B2 (en) | Medium discharge device | |
| CN214756951U (en) | Temperature control system for tin bath physical simulation | |
| CN108489684B (en) | Deep hole plate liquid leakage detection device | |
| CN106882918B (en) | Micro-nano nozzle forging instrument and forging method | |
| CN219998174U (en) | Pod conveying and opening device for pod | |
| CN214950595U (en) | Reaction furnace for two-dimensional crystals | |
| CN113156057A (en) | Smoke detector calibration device | |
| CN209961635U (en) | Heating furnace natural falling structure |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |