CN106983674A - A kind of water-soluble myprozine composition and preparation method and application - Google Patents
A kind of water-soluble myprozine composition and preparation method and application Download PDFInfo
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- CN106983674A CN106983674A CN201611053598.6A CN201611053598A CN106983674A CN 106983674 A CN106983674 A CN 106983674A CN 201611053598 A CN201611053598 A CN 201611053598A CN 106983674 A CN106983674 A CN 106983674A
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- cyclodextrin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/738—Cyclodextrins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/22—Carbohydrates or derivatives thereof
- C11D3/221—Mono, di- or trisaccharides or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
Abstract
The invention discloses a kind of water-soluble myprozine composition and preparation method and application, said composition includes the component of following parts by weight：0.1 5 parts of natamycin, solubilized 1.0 60 parts of stabilizer, 0 0.5 parts of preservative, 10 90 parts of solvent, or also include 10 90 parts of carrier auxiliary agent.The present composition all has good inhibiting effect under relatively low addition to mould, saccharomycete.Product applicability is wide, and usage amount is small, and cost is low, and used solubilized stabilizer is cyclodextrin pharmaceutic adjuvant, greatly enhances the solubility of natamycin, makes composition excellent aqueous solubility, is adapted to various occasions and uses.Especially, the present invention adds carrier auxiliary agent on the basis of solubilising, is the raw-food materials such as sorbierite, harmless, and greatly improves the stability of composition, the use requirement for making it more meet daily chemical products.
Description
Technical field
The present invention relates to a kind of water-soluble myprozine composition and preparation method and application.
Background technology
Natamycin (Natamycin) is a kind of effectively to suppress and kill in the big ring of the polyene of mould and saccharomycete
The antibacterial material of esters.Its inhibition to fungi is excellent, and most moulds are 0.5~6 μ g/mL in natamycin concentration
When be suppressed, indivedual strains are suppressed in 10~25 μ g/mL, and most saccharomycete are suppressed in 1~5 μ g/mL, and not
See that fungi produces drug resistance report to it.It has been widely used for food, agricultural as international biological preservative and raised
Material field it is mould proof.Due to its extremely low water solubility (about 30~50ppm), its occupation mode in existing application is limited,
Its suspension is mainly sprayed on product surface and suppresses the purpose that fungi grows so as to reach.
Suspension, micro emulsion liquid and preparation method thereof and its complex composition of existing natamycin be related to mostly food industry,
The application in the fields such as agricultural.Natamycin the fields such as daily chemical products, wet tissue application also in starting stage, pertinent literature report
Road and patent are also more rare.
To overcome the shortcomings of the existing technology, the present invention provides a kind of composition of water-soluble natamycin, it is safe and efficient, completely
Water-soluble, convenient use can be added in the big product of the water content such as wet tissue, toner, facial mask, it can also be used to household articles for washing
It is mould proof, expand use scope of the natamycin in daily chemical products and wet tissue.
The content of the invention
The present invention provides a kind of containing natamycin, solubilized stabilizer, the composition of carrier auxiliary agent (and solvent), can prepare
For liquid or solid form, solubility of the natamycin in water is improved by the effect of solubilizer, is added to it
In the daily chemical products and household articles of the high-moistures such as wet tissue, facial mask, said composition has excellent to common mould and saccharomycete
Inhibitory or killing effect, antimicrobial spectrum is wide, and effect stability, usage amount is low.
Technical solution of the present invention is as follows：
A kind of water-soluble myprozine composition, includes the component of following parts by weight：
0.1-5 parts of natamycin, solubilized stabilizer 1.0-60 parts, 0-0.5 parts of preservative, 10-90 parts of solvent, or also wrap
Include 10-90 parts of carrier auxiliary agent；
The solubilized stabilizer includes hydroxypropyl-β-cyclodextrin, hydropropyl-y-cyclodextrin, methyl-B-cyclodextrin, hydroxyl fourth
One or more of mixtures in group-beta-cyclodextrin, carboxymethyl-beta-cyclodextrin etc.；
The preservative includes imidazolidinyl urea, DMDMH, Bronopol, Phenoxyethanol, (C12-C18) alkyl
One or more of mixtures in trimethyl ammonium chloride, benzethonium chloride, polyaminopropyl biguanide etc.；
The solvent includes one or more of mixtures in propane diols, glycerine, butanediol, polyethylene glycol, water；
The carrier auxiliary agent includes one or more of mixtures in sorbierite, xylitol, mannitol etc..
Preferably, the water-soluble myprozine composition, includes the component of following parts by weight：Natamycin 0.25-1.5
Part, solubilized stabilizer 10-45 parts, 0-0.5 parts of preservative, 30-80 parts of solvent, or also include 30-80 parts of carrier auxiliary agent.
It is further preferred that the water-soluble myprozine composition, includes the component of following parts by weight：Natamycin 0.5-
1.2 parts, solubilized stabilizer 15-40 parts, 0-0.5 parts of preservative, 30-80 parts of solvent, or also include 30-80 parts of carrier auxiliary agent.
Water-soluble myprozine composition of the present invention, when solvent for use is not water, can omit preservative.
Water-soluble myprozine composition of the present invention can be liquid, also can further be prepared as solid-state, such as solid state powder.
It is further preferred that the water-soluble myprozine composition, it is liquid formulation, includes the group of following parts by weight
Point：0.5-1.2 parts of natamycin, solubilized stabilizer 25-30 parts, 0-0.1 parts of preservative, 55-70 parts of solvent, carrier auxiliary agent 10-
20 parts；The solubilized stabilizer be hydroxyl fourth group-beta-cyclodextrin, methyl-B-cyclodextrin, one kind in carboxymethyl-beta-cyclodextrin or
Two kinds of mixture；The preservative is C12Alkyl trimethyl ammonium chloride or Bronopol；The solvent includes propane diols, the third three
One or more of mixtures in alcohol, butanediol, polyethylene glycol, water；The carrier auxiliary agent is sorbierite, xylitol, sweet dew
The mixture of one or both of alcohol.
Wherein, one of specific embodiment is：A kind of water-soluble myprozine composition, is liquid formulation, including with
The component of lower parts by weight：0.8 part of natamycin, 15 parts of hydroxyl fourth group-beta-cyclodextrin, 10 parts of methyl-B-cyclodextrin, C12 alkyl three
0.1 part of ammonio methacrylate, 34.1 parts of propane diols, 10 parts of glycerine, 10 parts of water, 15 parts of xylitol, 5 parts of mannitol.
Wherein, one of specific embodiment is：A kind of water-soluble myprozine composition, is liquid formulation, including with
The component of lower parts by weight：0.5 part of natamycin, 15 parts of hydroxyl fourth group-beta-cyclodextrin, methyl-β-ring pastes 5 parts, Bronopol 0.02
Part, 10 parts of polyethylene glycol, 10 parts of butanediol, 49.5 parts of water, 10 parts of mannitol.
Wherein, one of specific embodiment is：A kind of water-soluble myprozine composition, is liquid formulation, including with
The component of lower parts by weight：1.2 parts of natamycin, 30 parts of carboxymethyl-beta-cyclodextrin, 20 parts of butanediol, 38.8 parts of polyethylene glycol, wood
10 parts of sugar alcohol.
It is further preferred that the water-soluble myprozine composition, it is solid formulation, includes the group of following parts by weight
Point：0.3-1.0 parts of natamycin, solubilized stabilizer 15-25 parts, 0.2-0.5 parts of preservative, 50-80 parts of solvent, carrier auxiliary agent 5-
30 parts；The solubilized stabilizer be hydroxypropyl-β-cyclodextrin, carboxymethyl-beta-cyclodextrin, hydroxyl fourth group-beta-cyclodextrin, methyl-β-
The mixture of one or both of cyclodextrin；The preservative is in DMDMH, benzethonium chloride, polyaminopropyl biguanide
One or two kinds of mixtures；The solvent is water；The carrier auxiliary agent is one kind in sorbierite, xylitol, mannitol
Or two kinds of mixture.
Wherein, one of specific embodiment is：A kind of water-soluble myprozine composition, is solid formulation, including with
The component of lower parts by weight：In 0.3 part of natamycin, 10 parts of hydroxypropyl-β-cyclodextrin, 5 parts of carboxymethyl-beta-cyclodextrin, DMDM second
0.3 part of uride, 79.4 parts of water, 5 parts of sorbierite.
Wherein, one of specific embodiment is：A kind of water-soluble myprozine composition, is solid formulation, including with
The component of lower parts by weight：1.0 parts of natamycin, 15 parts of carboxymethyl-beta-cyclodextrin, 10 parts of hydroxyl fourth group-beta-cyclodextrin, benzethonium chloride
0.01 part, 0.2 part of polyaminopropyl biguanide, 68.8 parts of water, 5 parts of sorbierite.
Wherein, one of specific embodiment is：A kind of water-soluble myprozine composition, is solid formulation, including with
The component of lower parts by weight：0.6 part of natamycin, 15 parts of hydroxypropyl-β-cyclodextrin, methyl-β-ring pastes 5 parts, DMDMH
0.2 part, 0.3 part of polyaminopropyl biguanide, 48.9 parts of water, 15 parts of sorbierite, 15 parts of xylitol.
It is further preferred that its raw material gross weight of water-soluble myprozine composition of the present invention is 100 parts.
Composition of the present invention can be prepared by existing conventional method.
Specifically, the preparation method of the liquid forms composition includes：Stirred after natamycin and solvent are added into container
Mix uniform, be warming up to 50-80 DEG C, add solubilized stabilizer, and/or carrier auxiliary agent, added after stirring to all dissolvings anti-
Rotten agent stirring and dissolving, is produced.
Specifically, the preparation method of the solid dosage composition includes：Natamycin and water are added after container and stirred
Uniformly, 50-80 DEG C is warming up to, solubilized stabilizer and carrier auxiliary agent is added, adding preservative after stirring to all dissolvings stirs
Dissolving, freeze-drying or spray drying are mixed, is produced.
It is used for personal care articles, household articles for washing or wet tissue present invention additionally comprises above-mentioned water-soluble myprozine composition
Deng purposes.
Present invention additionally comprises the personal care articles containing above-mentioned water-soluble myprozine composition, household articles for washing or wet
The products such as towel.
Water-soluble myprozine composition of the present invention is in the products such as personal care articles, household articles for washing or wet tissue
Addition be generally 0.3-0.8% (weight ratio).
The water-soluble myprozine composition can be used for various personal care articles, household articles for washing, is especially incorporated into and contains
Mildew-proof function is played in the higher product of water.Personal care articles of the present invention, household articles for washing etc. include but is not limited to cream
The products such as frost, emulsion, mildy wash, gel, shampoo, shower cream, hand cleanser, toothpaste, toner, detergent, liquid detergent, wet tissue class.
The present composition all has good inhibiting effect under relatively low addition to mould, saccharomycete.Product
Applicability is wide, and usage amount is small, and cost is low, and used solubilized stabilizer is cyclodextrin pharmaceutic adjuvant, is largely improved
The solubility of natamycin, makes composition excellent aqueous solubility, is adapted to various occasions and uses.Especially, the present invention is in solubilising
On the basis of add carrier auxiliary agent, be the raw-food materials such as sorbierite, it is harmless, and greatly improve the stability of composition
(color, content), the use requirement for making it more meet daily chemical products.
Brief description of the drawings
Fig. 1 is natamycin changes of contents figure in the fluid sample of experimental example 4.
Fig. 2 is natamycin changes of contents figure in the solid sample of experimental example 4.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.It is unreceipted specific in embodiment
Technology or condition person, are carried out according to the technology or condition described by document in the art, or according to product description.It is used
Reagent or the unreceipted production firm person of instrument, are the conventional products that can be commercially available by regular distributor.
Following used natamycin content is 95.7%, purchased from Beijing DongFang RuiDe Bioisystech Co., Ltd.
Indicated Ru not special, % as described below refers both to percentage by weight.
Embodiment 1-13
Water-soluble myprozine composition, it is liquid formulation, and its component includes natamycin, solubilized stabilizer, anti-corrosion
Agent, solvent, or also include carrier auxiliary agent；Each component consumption proportion is shown in Table 1.
Preparation method includes：Stirred after natamycin and solvent are added into container, be warming up to 50-80 DEG C, add
Solubilized stabilizer, and/or carrier auxiliary agent, insulation are cooled to less than 45 DEG C after stirring to all dissolvings, add preservative stirring
Dissolving, produces the water-soluble myprozine composition of stable transparent.
Embodiment 14-18
Water-soluble myprozine composition, it is liquid formulation, and its component includes natamycin, solubilized stabilizer, anti-corrosion
Agent, water, and carrier auxiliary agent；Each component consumption proportion is shown in Table 4# (i.e. embodiment 14) in 1,7# (i.e. embodiment 15), 10# (i.e.
Embodiment 16), 11# (i.e. embodiment 17) and 13# (i.e. embodiment 18) formula.
Preparation method includes：Stirred after natamycin and water are added into container, be warming up to 50-80 DEG C, add increasing
Molten stabilizer and carrier auxiliary agent, insulation is cooled to less than 45 DEG C after stirring to all dissolvings, adds preservative stirring and dissolving,
Freeze-drying is spray-dried, and produces solid state powder.
The water-soluble natamycin formula each component content (parts by weight) of table 1
Experimental example 1
Embodiment 3 (being formulated 3#), embodiment 5 (being formulated 5#), embodiment 9 (being formulated 9#), embodiment are investigated respectively
(25 DEG C) dissolubility in water under 12 (being formulated 12#) water solubility myprozine composition normal temperature.
Experimental method：Above laboratory sample is weighed respectively, is added in 100g water, is fully mixed；Then put respectively in normal temperature
Put and observed after refrigerating placement 24 hours within 72 hours, 4 DEG C.Using natamycin as control sample.It the results are shown in Table 2.
Table 2
The result of table 2 shows that each laboratory sample can be completely dissolved in water and under each condition of storage under test conditions
Do not separate out, illustrate that resulting composition of the present invention has good water solubility, substantially increase reality of the natamycin in water molten
Xie Du.To ensure fungistatic effect, addition of the natamycin in daily chemical products typically should be 0.005~0.03% (with activity
Thing meter), the need for the present composition can meet practical application completely.
Experimental example 2
It is water-soluble that embodiment 14 (being formulated 4#), embodiment 15 (being formulated 7#), embodiment 16 (being formulated 10#) are investigated respectively
(25 DEG C) dissolubility in water under property myprozine composition normal temperature.
Experimental method：Above laboratory sample is weighed respectively, is added in 100g water, is fully mixed；Then put respectively in normal temperature
Put and observed after refrigerating placement 24 hours within 72 hours, 4 DEG C.Using natamycin as control sample.It the results are shown in Table 3.
Table 3
The result of table 3 shows that each laboratory sample can be completely dissolved in water and under each condition of storage under test conditions
Do not separate out, illustrate that the present composition substantially increases actual solubility of the natamycin in water.To ensure fungistatic effect, receive
His addition of the mycin in daily chemical products typically should be in 0.005~0.03% (with actives basis), and the present composition is complete
The need for practical application being met.
Experimental example 3
The effect of carrier auxiliary agent in the composition is investigated, is placed before and after the addition of comparison vehicle auxiliary agent at 54 ± 1 DEG C of composition
The change of color, the results are shown in Table 4 after 14 days.
Control sample 1：Do not include carrier auxiliary agent (i.e. sorb with its component that differs only in of embodiment 14 (being formulated 4#)
Alcohol).
Control sample 2：Do not include carrier auxiliary agent (i.e. sorb with its component that differs only in of embodiment 15 (being formulated 7#)
Alcohol).
Control sample 3：Do not include carrier auxiliary agent (i.e. mountain with its component that differs only in of embodiment 16 (being formulated 10#)
Pears alcohol and xylitol).
Control sample 4：Do not include carrier auxiliary agent (i.e. xylose with its component that differs only in of embodiment 3 (being formulated 3#)
Alcohol and mannitol).
Control sample 5：Do not include carrier auxiliary agent (i.e. sweet dew with its component that differs only in of embodiment 5 (being formulated 5#)
Alcohol).
Control sample 6：Do not include carrier auxiliary agent (i.e. xylose with its component that differs only in of embodiment 9 (being formulated 9#)
Alcohol).
Influence of the carrier auxiliary agent of table 4 to composition color
Table 4 is it can be seen that the composition of addition carrier auxiliary agent shows more preferable stability (color).
Experimental example 4
The effect of carrier auxiliary agent in the composition is investigated, fluid sample (control sample 1, embodiment 7) and solid sample is taken
Each 10 grams of (control sample 2, embodiment 16) detects the change of wherein natamycin content in being placed at 54 ± 1 DEG C after 14 days, tie
Fruit is shown in Table 5, Fig. 1 (natamycin changes of contents figure in fluid sample), Fig. 2 (natamycin changes of contents figure in solid sample).
Control sample 1：Difference with embodiment 7 (being formulated 7#) is that its component does not include carrier auxiliary agent sorbierite, i.e.,
Sorbierite is replaced with the water with equivalent.
Control sample 2：Difference with embodiment 16 (being formulated 10#) be its component include carrier auxiliary agent sorbierite and
Xylitol, i.e., replace sorbierite with the hydroxypropyl-β-cyclodextrin of equivalent, and xylitol is replaced with methyl-β-ring paste of equivalent.
Table 5
Table 5 and Fig. 1, Fig. 2 can be seen that the composition of addition carrier auxiliary agent shows more preferable stability (content).
Experimental example 5
Anti- (suppression) bacterium experiment (I) --- minimum inhibitory concentration (MIC value) is determined.
Embodiment 3 (being formulated 3#), embodiment 5 (being formulated 5#), embodiment 9 (being formulated 9#) water are determined by suspension method
Dissolubility myprozine composition minimum inhibitory concentration (MIC value), the results are shown in Table 6.
The minimum inhibitory concentration of table 6 (MIC value)
By taking embodiment 3 (being formulated 3#) as an example, it is left for 2.5ppm that its MIC value to aspergillus niger is converted to natamycin content
The right side, is 2ppm or so to the MIC of Trichoderma viride, this and number of the document report natamycin to the minimum inhibitory concentration of a variety of moulds
According to being consistent.
Experimental example 6
Anti- (suppression) bacterium experiment (II) --- minimum inhibitory concentration (MIC value) is determined.
Embodiment 14 (being formulated 4#), embodiment 15 (being formulated 7#), embodiment 16 are determined by suspension method (to be formulated
10#) water-soluble myprozine composition minimum inhibitory concentration (MIC value), the results are shown in Table 7.
The solid composite minimum inhibitory concentration (MIC value) of table 7
Solid dosage is equivalent to improving the content of natamycin in the composition, therefore relative MIC value reduction, antibacterial effect
The corresponding enhancing of fruit, can reduce product addition when using.
Experimental example 7
Inhibitor effectiveness test (challenge experiment I).
The protective system efficiency evaluation method recommended with reference to cosmetics spices and essence association of the U.S. (CTFA), carries out 28 days lists
It is secondary to connect the experiment of bacterium challenge.
1. test strain：Bacterium (E. coli, staphylococcus aureus S.aureus, pseudomonas aeruginosa
P.aeruginosa；Fungi (aspergillus niger A.niger, candida albicans bacterium C.albicans).It is inoculated with using Mixed Microbes, bacterium mixes
Close bacterium suspension 107CFU/mL；Fungi Mixed Microbes suspension 106CFU/mL。
2. test specimen：By formula (marine algae extract 0.25%, sodium ascorbyl phosphate 1.0%, propane diols 10.0%,
Hydroxyethyl cellulose 0.4%, hyaluronic acid 0.01%, Preservative qs, deionized water add to 100%) preparation face pack prescription, its
Formulation content addition described in A, B, C, D (and sample number into spectrum is used as using protective system numbering) in middle protective system difference according to the form below 8,
To investigate the fungistatic effect of different protective systems.
Table 8 is formulated each protective system addition (weight percentage)
3. sample inoculation：Above-mentioned each 2 parts of 100 grams of cosmetics A, B, C, D is weighed respectively, is separately added into 1mL mixed cells and is hanged
Turbid liquid and 1mL mixed fungus suspension, is well mixed, by sample in the preservation of (22.5 ± 2.5 DEG C) of room temperature, and respectively at 0,2,7,
14th, 21, the clump count in 28 days detection cosmetics, to judge protective system efficiency.
4. result of the test
In inoculation 0,2,7,14, sampling detections in 21 and 28 days, it the results are shown in Table 9：
The composition Microbial Challenge result of the test of table 9
The result of table 9 shows that A groups and protective system of the D groups containing composition, by challenge, are made in Fungal challenges experiment
It is equally matched with protective system of the B groups containing IPBC with effect；D group system active matter contents are slightly lower, but also pass through examination at the 7th day
Test, compared to 21 days of C group protective systems by challenge, the anti-mold effect of composition is more excellent.
Experimental example 8
Inhibitor effectiveness test (challenge experiment II).
The protective system efficiency evaluation method recommended with reference to cosmetics spices and essence association of the U.S. (CTFA), carries out 28 days lists
It is secondary to connect the experiment of bacterium challenge.
1. test strain and bacterium Mixed Microbes suspension, fungi Mixed Microbes suspension are with experimental example 7.
2. test specimen：By formula (propane diols 2.0%, cactus dry powder 0.01%, water-soluble lanolin 0.01%, second
It is wet that edetate disodium 0.08%, triethanolamine are a small amount of, Preservative qs, deionized water add to 100%) the common clean skin of preparation
Towel liquid, wherein protective system difference according to the form below in described in A, B, C formulation content addition (and using protective system numbering compiled as sample
Number, it is shown in Table 10), to investigate the fungistatic effect of different protective systems.
Each protective system addition of table 10
| Sample | Protective system |
| A | Imidazolidinyl urea 0.2%, embodiment 14 (being formulated 4#) 0.3% |
| B | Imidazolidinyl urea 0.2%, iodopropynyl butyl formate 0.01% |
| C | Phenoxyethanol 0.5%, dehydroactic acid 0.06%, sorbic acid 0.04% |
3. sample inoculation：Above-mentioned each 2 parts of 100 grams of wet tissue liquid A, B, C is weighed respectively, is separately added into the mixing of 1mL bacteriums suspended
Liquid and 1mL fungi mixing suspensions, are well mixed, by sample in the preservation of (22.5 ± 2.5 DEG C) of room temperature, and respectively at 0,2,7,
14th, 21, the clump count in 28 days detection cosmetics, to judge inhibitor effectiveness.
4. result of the test
In inoculation 0,2,7,14, sampling detections in 21 and 28 days, it the results are shown in Table 11：
The composition Microbial Challenge result of the test of table 11
The result of table 11 shows that A groups pass through for the 2nd day in Fungal challenges experiment with B groups protective system, and display water solubility is received
The anti-mold effect of his mycin composition can compare favourably with IPBC, and with preferable bacteriological protection effect；A groups can be seen compared with C groups
Go out, no matter in bacterium or fungi, the inhibitory action of composition is superior to the combination of polyalcohol and organic acid.
Experimental example 9
The made facial mask of experimental example 7 is added using control sample 1 in experimental example 3 and embodiment 14 (being formulated 4#) as preservative
Formula of liquid, addition is 0.3%；Control sample 4 in experimental example 3 and embodiment 3 (being formulated 3#) are added as preservative real
Test in the made wet tissue liquid of example 8, addition is 0.5%, placed 180 days for (20-30 DEG C) under normal temperature, observe color change and detect it
Colony counts, the results are shown in Table 12：
Table 12
The composition of the result of table 12 display addition carrier auxiliary agent embodies when end product is made in terms of color and effect
Go out good stability, be more suitable for the application of daily use chemicals industry.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (10)
1. a kind of water-soluble myprozine composition, it is characterised in that include the component of following parts by weight：Natamycin 0.1-5
Part, solubilized stabilizer 1.0-60 parts, 0-0.5 parts of preservative, 10-90 parts of solvent, or also include 10-90 parts of carrier auxiliary agent；
The solubilized stabilizer include hydroxypropyl-β-cyclodextrin, hydropropyl-y-cyclodextrin, methyl-B-cyclodextrin, hydroxyl butyl-
One or more of mixtures in beta-schardinger dextrin, carboxymethyl-beta-cyclodextrin；
The preservative includes imidazolidinyl urea, DMDMH, Bronopol, Phenoxyethanol, (C12-C18) alkyl trimethyl
One or more of mixtures in ammonium chloride, benzethonium chloride, polyaminopropyl biguanide；
The solvent includes one or more of mixtures in propane diols, glycerine, butanediol, polyethylene glycol, water；
The carrier auxiliary agent includes one or more of mixtures in sorbierite, xylitol, mannitol.
2. water-soluble myprozine composition according to claim 1, it is characterised in that include the component of following parts by weight：
0.25-1.5 parts of natamycin, solubilized stabilizer 10-45 parts, 0-0.5 parts of preservative, 30-80 parts of solvent, or also include carrier
30-80 parts of auxiliary agent；
Preferably, the water-soluble myprozine composition, includes the component of following parts by weight：0.5-1.2 parts of natamycin, increases
Molten stabilizer 15-40 parts, 0-0.5 parts of preservative, 30-80 parts of solvent, or also include 30-80 parts of carrier auxiliary agent.
3. water-soluble myprozine composition according to claim 1, it is characterised in that it is liquid formulation, including following
The component of parts by weight：0.5-1.2 parts of natamycin, solubilized stabilizer 25-30 parts, 0-0.1 parts of preservative, 55-70 parts of solvent is carried
10-20 parts of body auxiliary agent；The solubilized stabilizer is in hydroxyl fourth group-beta-cyclodextrin, methyl-B-cyclodextrin, carboxymethyl-beta-cyclodextrin
One or two kinds of mixtures；The preservative is C12Alkyl trimethyl ammonium chloride or Bronopol；The solvent includes third
One or more of mixtures in glycol, glycerine, butanediol, polyethylene glycol, water；The carrier auxiliary agent is sorbierite, wood
The mixture of one or both of sugar alcohol, mannitol.
4. water-soluble myprozine composition according to claim 3, it is characterised in that include the component of following parts by weight：
0.8 part of natamycin, 15 parts of hydroxyl fourth group-beta-cyclodextrin, 10 parts of methyl-B-cyclodextrin, 0.1 part of C12 alkyl trimethyl ammonium chlorides,
34.1 parts of propane diols, 10 parts of glycerine, 10 parts of water, 15 parts of xylitol, 5 parts of mannitol；
Or, include the component of following parts by weight：0.5 part of natamycin, 15 parts of hydroxyl fourth group-beta-cyclodextrin, methyl-β-ring paste 5
Part, 0.02 part of Bronopol, 10 parts of polyethylene glycol, 10 parts of butanediol, 49.5 parts of water, 10 parts of mannitol；
Or include the component of following parts by weight：1.2 parts of natamycin, 30 parts of carboxymethyl-beta-cyclodextrin, 20 parts of butanediol gathers
38.8 parts of ethylene glycol, 10 parts of xylitol.
5. water-soluble myprozine composition according to claim 1, it is characterised in that it is solid formulation, including following
The component of parts by weight：0.3-1.0 parts of natamycin, solubilized stabilizer 15-25 parts, 0.2-0.5 parts of preservative, 50-80 parts of solvent,
5-30 parts of carrier auxiliary agent；The solubilized stabilizer is hydroxypropyl-β-cyclodextrin, carboxymethyl-beta-cyclodextrin, hydroxyl butyl-β-ring paste
The mixture of one or both of essence, methyl-B-cyclodextrin；The preservative is DMDMH, benzethonium chloride, poly- ammonia
The mixture of one or both of propyl group biguanides；The solvent is water；The carrier auxiliary agent is sorbierite, xylitol, sweet dew
The mixture of one or both of alcohol.
6. water-soluble myprozine composition according to claim 5, it is characterised in that include the component of following parts by weight：
0.3 part of natamycin, 10 parts of hydroxypropyl-β-cyclodextrin, 5 parts of carboxymethyl-beta-cyclodextrin, 0.3 part of DMDMH, water 79.4
Part, 5 parts of sorbierite；
Or, include the component of following parts by weight：1.0 parts of natamycin, 15 parts of carboxymethyl-beta-cyclodextrin, hydroxyl butyl-β-ring paste
10 parts of essence, 0.01 part of benzethonium chloride, 0.2 part of polyaminopropyl biguanide, 68.8 parts of water, 5 parts of sorbierite；
Or, include the component of following parts by weight：0.6 part of natamycin, 15 parts of hydroxypropyl-β-cyclodextrin, methyl-β-ring paste 5
Part, 0.2 part of DMDMH, 0.3 part of polyaminopropyl biguanide, 48.9 parts of water, 15 parts of sorbierite, 15 parts of xylitol.
7. the preparation method of any one of the claim 1-6 water-soluble myprozine compositions, it is characterised in that including：Will
Natamycin and solvent are stirred after adding container, are warming up to 50-80 DEG C, add solubilized stabilizer, and/or carrier is helped
Agent, adds preservative stirring and dissolving after stirring to all dissolvings, produces.
8. prepared by any one of the claim 1-6 water-soluble myprozine compositions or claim 7 methods described water-soluble
Property myprozine composition be used for personal care articles, household articles for washing or wet tissue purposes.
9. prepared containing any one of the claim 1-6 water-soluble myprozine compositions or claim 7 methods described
Personal care articles, household articles for washing or the wet tissue of water-soluble myprozine composition.
10. personal care articles, household articles for washing or wet tissue described in purposes according to claim 8 or claim 9,
The addition of wherein described water-soluble myprozine composition is 0.3-0.8%.
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| CN106983674B (en) | 2022-02-18 |
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