CN106977273A - The method that the regulation and control of walnut protein zymolyte prepare the trace-element fertilizer containing nanometer selenium - Google Patents
The method that the regulation and control of walnut protein zymolyte prepare the trace-element fertilizer containing nanometer selenium Download PDFInfo
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- CN106977273A CN106977273A CN201710189748.4A CN201710189748A CN106977273A CN 106977273 A CN106977273 A CN 106977273A CN 201710189748 A CN201710189748 A CN 201710189748A CN 106977273 A CN106977273 A CN 106977273A
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- protein zymolyte
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D5/00—Fertilisers containing magnesium
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Abstract
Trace-element fertilizer and preparation method containing nanometer selenium are prepared the present invention relates to a kind of regulation and control of walnut protein zymolyte, is comprised the following steps:By the drying of degreasing walnut dregs of rice raw material decontamination;By the degreasing walnut dregs of rice raw material of drying in enzymatic vessel with water mixed enzymolysis;Material is centrifuged, takes and walnut protein zymolyte is obtained after supernatant vacuum freeze drying;Take walnut protein zymolyte to be mixed with water, add sodium selenite and walnut protein zymolyte is made in vitamin C reaction --- nanometer selenium;By walnut protein zymolyte --- nanometer selenium is mixed to prepare the trace-element fertilizer of the present invention with magnesium sulfate, zinc sulfate, manganese sulfate, ferrous sulfate, borax, copper sulphate, sodium molybdate, silica, Arabic gum, water.Trace-element fertilizer of the present invention can not only improve the Se content and yield of crop, can improve crop quality with regulating crop growth, and preparation technology is simple, and reaction condition is gentle, and production cost is relatively low, with potential ecological, economy and social value.
Description
Technical field
The invention belongs to technical field of fertilizers, and in particular to a kind of walnut protein zymolyte regulation and control are prepared containing the micro- of nanometer selenium
The method of secondary element fertilizer.
Background technology
Selenium is one of the essential trace elements of the human body, with anticancer, improves the effect such as body immunity, cardioprotection, selenium
To tumour, Kaschin-Beck disease, diabetes, AIDS, cataract, breathing problem, low fecundity, Keshan disease, heavy metal poisoning
Deng also there is certain preventive and therapeutic action.Selenium can not be generated in human body, and the selenium that needed by human body is wanted can only be taken the photograph from food
Take.Selenium intake deficiency can cause damage to humans and animals, threaten human health.
China is a selenium deficiency big country.Soil investigation shows that the area of China 72% belongs to selenium deficiency and low selenium area, the whole nation
There is different degrees of selenium intake deficiency in about 2/3rds populations, drastically influence the healthy of local resident.Therefore, it is rich
The development of selenium fertilizer and the focus for being developed into the area researches such as agricultural sciences, Food Science and medical science of Se-rich farm products.Plant
It is key link in nature selenium ecological circulation and the direct sources of human body selenium.Plant has absorbs micro by root and blade
The characteristics of element, it can be absorbed using plant and be enriched with inorganic states selenium, and be translated into the form for being easy to plant absorption to utilize
The characteristic of selenium carries out the research of external source selenium-rich, using selenium-rich fertilizer plant reinforcing selenium-supply is had proved to be a kind of safety, low cost,
Efficiently convenient selenium-rich means.However, the concentration hardly possible of selenium is controlled in traditional selenium-enriched fertilizer, easily environment is polluted, therefore, opened
Send out nontoxic, selenium-enriched fertilizer product stably, safe has important social value and economic benefit.
The content of the invention
In view of the shortcomings of the prior art, prepared it is an object of the invention to provide a kind of regulation and control of walnut protein zymolyte and contain nanometer
Trace-element fertilizer of selenium and preparation method thereof.
The present invention is achieved through the following technical solutions:
A kind of method that walnut protein zymolyte regulation and control prepare the trace-element fertilizer containing nanometer selenium, comprises the following steps:
(1) preparation of walnut protein zymolyte
A, by degreasing walnut dregs of rice raw material decontamination dry for standby;
B, the degreasing walnut dregs of rice raw material of drying is taken in enzymatic vessel, be 1 by solid-liquid ratio:7 add water, mix, are pre-heated to
After 55 DEG C of hydrolysis temperature, trypsase is added, is digested under the conditions of pH 8.0;
C, go out step B resulting material boiling water baths 10min enzyme, and in 4 DEG C, 8000r/min centrifugation 20min are filtered, collected
Supernatant, as walnut protein zymolyte, vacuum freeze drying are preserved;
(2) preparation of walnut protein zymolyte --- nanometer selenium
A, walnut protein zymolyte freeze-dried powder obtained by step (1) is taken, by solid-liquid ratio 5:1 adds deionized water, and stirring is extremely
It is completely dissolved, adds 1mmol/L sodium selenite solutions, after whirlpool is mixed, 4mmol/L vitamin Cs (Vc) solution is added dropwise, 45 are placed in
Concussion is incubated 24h in DEG C constant-temperature table;
B, reaction solution obtained by step A is transferred in the bag filter that molecular cut off is 500u, 4 DEG C of lucifuges of distilled water are saturating
Analysis, first water is changed every 2h;The outer liquid of bag filter is detected through ICP-AES dialyses without stopping after plasma selenium, institute in bag filter
Sample is standby after walnut protein zymolyte --- nanometer selenium, vacuum freeze drying;
(3) preparation of trace-element fertilizer
1~5 part of magnesium sulfate, 0.5~2.5 part of zinc sulfate, 1~7 part of manganese sulfate, ferrous sulfate 2~8 are weighed by weight
Part, 2~8 parts of borax, 1~3 part of copper sulphate, 0.5~2.5 part of sodium molybdate, silica 1~5 part, 1~5 part of Arabic gum, water
80~90 parts, with the walnut protein zymolyte obtained by step (2) --- 2~8 parts of nanometer selenium freeze-dried powder is mixed, and is stirred to completely molten
Solution, pH value is adjusted between 5.5~6.5, is produced.
Preferably, it is 1 by enzyme amount and degreasing walnut dregs of rice raw material weight ratio in step (1):50~200 add trypsase,
Wherein enzyme activity is in terms of 100000U/g.
Preferably, enzymolysis time is 20~40 hours in step (1).
Preferably, sodium selenite solution, vitamin C (Vc) solution, walnut protein zymolyte solution press volume in step (2)
Than for Vc:Na2SeO3:Walnut protein zymolyte solution=4:4:1 ratio is mixed.
Each composition mechanism of action of the present invention is as follows:
Nanometer selenium:Selenium is one of essential trace element of human body, with enhancing body immunity, resistance free radical
Damage, diseases prevention and a variety of functions of regulation thyroid function etc., but selenium can not be manufactured in human body, can only outer portion mend
Fill.Secondly, selenium has significant facilitation to plant growth to a certain extent, such as improves crops quality (such as reduction nitre
Phosphate content, improves amino acid content etc.);Chlorophyll content is improved to strengthen the photosynthesis of crop;Remove mistake in plant
Free radical is measured, plant immune ability is improved by antioxidation, so as to strengthen resistance of the plant to pest and disease damage;Strengthen plant
Resistance of heavy metal, environmental contaminants and physiology adverse circumstance etc..Nanometer selenium is realized under certain condition by strong reductant
Selenate or selenite reduction are obtained, or using the microorganism with stronger reducing power, solvable selenium are reduced to
Biological nano selenium is prepared.Nanometer selenium is also high with bioresorbable in addition to the effect for possessing selenium, and energy high-efficient cleaning is except freely
Base, toxicity is low, the features such as active high, has huge application potential in terms of selenium-enriched fertilizer production and selenium contamination are administered.
Walnut protein zymolyte:Walnut protein zymolyte is made by trypsin digestion walnut dregs, and walnut dregs are walnut warps
Accessory substance after oil expression, contains at least 40% protein.Walnut protein is a kind of vegetable protein of high-quality, not only containing 18 kinds
Amino acid, and 8 kinds of essential amino acids contents of needed by human body are reasonable.Therefore, fertilizer is prepared with walnut protein zymolyte, not only may be used
To supplement nutrition needed for plant, promote plant strain growth, can also supplement amino acid needed by human body, be a kind of environment-friendly type fertilizer raw material.
Secondly, the characteristics of walnut protein zymolyte has the low, transport speed that consumes energy fast and is difficult to be saturated, can also improve the profit of amino acid
With rate, promote the synthesis of protein and the absorption of mineral element, protein zymolyte and selenizing are closed, pass through receiving that regulation and control are formed
The particle diameter of rice corpuscles, change its surface texture or charging property etc., can greatly improve water solubility, the bio-compatibility of nano-particle
With interbiotic recognition capability, prevent nanometer Se particle from reuniting or precipitating in preparation, preservation and application process.
Silicon:Silicon is the important component of plant, is the important nutrient that plant needs.Silicon is mainly deposited on blade
And leaf sheath epidermal cell, form silicified cell and cutin-silicon double-decker, can strengthen host plant cell wall mechanical strength and
Steadiness, so as to delay and resist the intrusion and extension of germ.Silicon processing can also dramatically increase plant leaf blade protective enzyme (peroxidating
Thing enzyme, polyphenol oxidase, phenylalanine lyase etc.) active and induction host's generation secondary metabolism resisting substance (such as protective plant protecting agent,
Polyphenol compound, lignin) so that the system of defense of activated plant, strengthens the resistivity to pathogen.Secondly, silicon is also
Can improve absorption of the plant to mineral nutrition, promote the robust growth of plant, enhancing plant drought, disease-resistant, pest-resistant, salt resistance,
The degeneration-resistant effect such as freeze proof, anti-metallic contamination, resistant to lodging, improves quality of agricultural product, is conducive to storing and transports.
Magnesium:Magnesium is the constituent of plant chlorophyll and polysome, is also the activator of a variety of enzymes, can promote to make object
The formation of interior vitamin A, C.Therefore, the photosynthesis of crops and the platform of protein can be promoted into raising farming using fertiliser containing magnesium
Produce amount, improves quality of agricultural product.According to another report, magnesium can also promote absorption of the plant to silicon, be conducive to preventing germ from invading.
Zinc:Zinc is the constituent of many enzymes, directly participates in the formation of auximone, to Substance P hydrolysis in plant,
Oxidation-reduction process plays an important role, can also catalytic proteins synthesis, promote seed maturity etc..
Manganese:Manganese participates in the assimilation of carbon dioxide in the metabolism of protein and inorganic acid, photosynthesis, point of carbohydrate
Formation of solution and carrotene, riboflavin and ascorbic acid etc..Manganese is to the formation of chlorophyll and accumulation transhipment, the seed of carbohydrate
Sprout, growth of seedling and plant is solid etc. good facilitation.Manganese is the activator of a variety of enzymes, to plant respiration, egg
White matter is decomposed, nitrate-nitrogen reduction is respectively provided with remarkable effect.
Iron:Iron participates in the synthesis of chlorophyll, and it is the composition of some enzymes of composition and protein, participates in the oxygen in plant
The manufacture of change-reduction process and carbohydrate.
Boron:Boron is the constituent of a variety of enzymes of plant, participates in the generation of synthesis, nitrogen and the carbohydrate of plant vivo protein
Thank.Boron can promote the normal operation of carbohydrate and the normal development of reproductive organs, improve the supply of oxygen, the development to root system
And the formation of fruit, seed has a major impact.Secondly, boron can also adjust absorption and oxidation-reduction process of the plant to moisture,
Also the formation of Legume nodule can be promoted.
Copper:Copper is the composition of a variety of oxidizing ferment of composition in plant, is also the catalyst of respiration, and it participates in chlorophyll
Synthesis and the metabolism of carbohydrate and protein.Copper also have improve chlorophyll stability ability, it is to avoid chlorophyll too early by
Destruction, is conducive to blade preferably to carry out photosynthesis.
Molybdenum:Molybdenum is present among biocatalyst, participates in symbiosis biological nitrogen fixation and the synthesis of protein, and promote photosynthetic
The intensity of effect.Secondly, plant relies on molybdenum and carries out nitrate reductase, eliminates activated aluminum in acid soil and accumulates and produce in plant
Raw toxic action.
Arabic gum:Aqueous colloidal, the resolvability with height is nontoxic, is a kind of carbohydrate polymer, can
As nanometer selenium protective agent, the bioactivity of nanometer selenium is maintained.
Beneficial effects of the present invention:
1st, the present invention closes protein zymolyte and selenizing, by regulating and controlling the particle diameter of formed nano-particle, changing its table
Face structure or charging property etc., can greatly improve water solubility, bio-compatibility and the interbiotic recognition capability of nano-particle, prevent
Only nanometer Se particle is reunited or precipitated in preparation, preservation and application process.
2nd, nanometer selenium of the present invention is nontoxic, stably, with higher bioactivity, is easily absorbed by plants and turns
Fortune, the bioactive functions of Organic Selenium and inorganic selenium are given full play in plant, Available Selenium is reduced and is inhaled in soil
The attached or fixed and environmental pollution as caused by fertilizer loss, so as to improve the utilization rate of selenium.
3rd, trace-element fertilizer of the invention can not only improve the Se content and yield of crops, can also adjust farming
Thing grows, and improves crop quality, with potential ecological, economy and social value.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1
A kind of method that walnut protein zymolyte regulation and control prepare the trace-element fertilizer containing nanometer selenium, comprises the following steps:
(1) preparation of walnut protein zymolyte
A, by degreasing walnut dregs of rice raw material decontamination dry for standby;
B, the degreasing walnut dregs of rice raw material of drying is taken in enzymatic vessel, be 1 by solid-liquid ratio:7 add water, mix, are pre-heated to
It is 1 by enzyme amount and degreasing walnut dregs of rice raw material weight ratio after 55 DEG C of hydrolysis temperature:50 add trypsase, under the conditions of pH 8.0
Enzymolysis 20 hours;
C, go out step B resulting material boiling water baths 10min enzyme, and in 4 DEG C, 8000r/min centrifugation 20min are filtered, collected
Supernatant, as walnut protein zymolyte, vacuum freeze drying are preserved.
(2) preparation of walnut protein zymolyte --- nanometer selenium
A, walnut protein zymolyte freeze-dried powder obtained by step (1) is taken, by solid-liquid ratio 5:1 adds deionized water, and stirring is extremely
It is completely dissolved, adds 1mmol/L sodium selenite solutions, after whirlpool is mixed, 4mmol/L vitamin Cs (Vc) solution is added dropwise, wherein,
Sodium selenite solution, vitamin C (Vc) solution, walnut protein zymolyte liquor capacity ratio are 4:4:1, it is placed in 45 DEG C of constant-temperature tables
Middle concussion is incubated 24h;
B, reaction solution obtained by step A is transferred in the bag filter that molecular cut off is 500u, 4 DEG C of lucifuges of distilled water are saturating
Analysis, first water is changed every 2h;The outer liquid of bag filter is detected through ICP-AES dialyses without stopping after plasma selenium, institute in bag filter
Sample is standby after walnut protein zymolyte --- nanometer selenium, vacuum freeze drying.
(3) preparation of trace-element fertilizer
1 part of magnesium sulfate, 0.5 part of zinc sulfate, 7 parts of manganese sulfate, 2 parts of ferrous sulfate, 8 parts of borax, sulfuric acid are weighed by weight
1 part of copper, 2.5 parts of sodium molybdate, silica 1 part, 1 part of Arabic gum, 80 parts of water, are digested with the walnut protein obtained by step (2)
The 8 parts of mixing of thing --- nanometer selenium freeze-dried powder, stirring is to being completely dissolved, and pH value is adjusted between 5.5~6.5, is produced.
Embodiment 2
A kind of method that walnut protein zymolyte regulation and control prepare the trace-element fertilizer containing nanometer selenium, comprises the following steps:
(1) preparation of walnut protein zymolyte
A, by degreasing walnut dregs of rice raw material decontamination dry for standby;
B, the degreasing walnut dregs of rice raw material of drying is taken in enzymatic vessel, be 1 by solid-liquid ratio:7 add water, mix, are pre-heated to
It is 1 by enzyme amount and degreasing walnut dregs of rice raw material weight ratio after 55 DEG C of hydrolysis temperature:100 add trypsase, under the conditions of pH 8.0
Enzymolysis 30 hours;
C, go out step B resulting material boiling water baths 10min enzyme, and in 4 DEG C, 8000r/min centrifugation 20min are filtered, collected
Supernatant, as walnut protein zymolyte, vacuum freeze drying are preserved.
(2) preparation of walnut protein zymolyte --- nanometer selenium
A, walnut protein zymolyte freeze-dried powder obtained by step (1) is taken, by solid-liquid ratio 5:1 adds deionized water, and stirring is extremely
It is completely dissolved, adds 1mmol/L sodium selenite solutions, after whirlpool is mixed, 4mmol/L vitamin Cs (Vc) solution is added dropwise, wherein,
Sodium selenite solution, vitamin C (Vc) solution, walnut protein zymolyte liquor capacity ratio are 4:4:1, it is placed in 45 DEG C of constant-temperature tables
Middle concussion is incubated 24h;
B, reaction solution obtained by step A is transferred in the bag filter that molecular cut off is 500u, 4 DEG C of lucifuges of distilled water are saturating
Analysis, first water is changed every 2h;The outer liquid of bag filter is detected through ICP-AES dialyses without stopping after plasma selenium, institute in bag filter
Sample is standby after walnut protein zymolyte --- nanometer selenium, vacuum freeze drying.
(3) preparation of trace-element fertilizer
3 parts of magnesium sulfate, 1.5 parts of zinc sulfate, 4 parts of manganese sulfate, 5 parts of ferrous sulfate, 5 parts of borax, sulfuric acid are weighed by weight
2 parts of copper, 1.5 parts of sodium molybdate, 3 parts of silica, 3 parts of Arabic gum, 85 parts of water, are digested with the walnut protein obtained by step (2)
The 5 parts of mixing of thing --- nanometer selenium freeze-dried powder, stirring is to being completely dissolved, and pH value is adjusted between 5.5~6.5, is produced.
Embodiment 3
A kind of method that walnut protein zymolyte regulation and control prepare the trace-element fertilizer containing nanometer selenium, comprises the following steps:
(1) preparation of walnut protein zymolyte
A, by degreasing walnut dregs of rice raw material decontamination dry for standby;
B, the degreasing walnut dregs of rice raw material of drying is taken in enzymatic vessel, be 1 by solid-liquid ratio:7 add water, mix, are pre-heated to
It is 1 by enzyme amount and degreasing walnut dregs of rice raw material weight ratio after 55 DEG C of hydrolysis temperature:200 add trypsase, under the conditions of pH 8.0
Enzymolysis 40 hours;
C, go out step B resulting material boiling water baths 10min enzyme, and in 4 DEG C, 8000r/min centrifugation 20min are filtered, collected
Supernatant, as walnut protein zymolyte, vacuum freeze drying are preserved.
(2) preparation of walnut protein zymolyte --- nanometer selenium
A, walnut protein zymolyte freeze-dried powder obtained by step (1) is taken, by solid-liquid ratio 5:1 adds deionized water, and stirring is extremely
It is completely dissolved, adds 1mmol/L sodium selenite solutions, after whirlpool is mixed, 4mmol/L vitamin Cs (Vc) solution is added dropwise, wherein,
Sodium selenite solution, vitamin C (Vc) solution, walnut protein zymolyte liquor capacity ratio are 4:4:1, it is placed in 45 DEG C of constant-temperature tables
Middle concussion is incubated 24h;
B, reaction solution obtained by step A is transferred in the bag filter that molecular cut off is 500u, 4 DEG C of lucifuges of distilled water are saturating
Analysis, first water is changed every 2h;The outer liquid of bag filter is detected through ICP-AES dialyses without stopping after plasma selenium, institute in bag filter
Sample is standby after walnut protein zymolyte --- nanometer selenium, vacuum freeze drying.
(3) preparation of trace-element fertilizer
5 parts of magnesium sulfate, 2.5 parts of zinc sulfate, 1 part of manganese sulfate, 8 parts of ferrous sulfate, 2 parts of borax, sulfuric acid are weighed by weight
3 parts of copper, 0.5 part of sodium molybdate, 5 parts of silica, 5 parts of Arabic gum, 90 parts of water, are digested with the walnut protein obtained by step (2)
The 2 parts of mixing of thing --- nanometer selenium freeze-dried powder, stirring is to being completely dissolved, and pH value is adjusted between 5.5~6.5, is produced.
Comparative example 1
Without walnut protein zymolyte and selenium in trace-element fertilizer, its preparation method is as follows:
3 parts of magnesium sulfate, 1.5 parts of zinc sulfate, 4 parts of manganese sulfate, 5 parts of ferrous sulfate, 5 parts of borax, sulfuric acid are weighed by weight
2 parts of copper, 1.5 parts of sodium molybdate, 3 parts of silica, 3 parts of Arabic gum, 85 parts of water, alleged raw material is mixed, and is stirred to completely molten
Solution, pH value is adjusted between 5.5~6.5, is produced.
Comparative example 2
Without selenium in trace-element fertilizer, its preparation method is as follows:
(1) preparation of walnut protein zymolyte
A, by degreasing walnut dregs of rice raw material decontamination dry for standby;
B, the degreasing walnut dregs of rice raw material of drying is taken in enzymatic vessel, be 1 by solid-liquid ratio:7 add water, mix, are pre-heated to
It is 1 by enzyme amount and degreasing walnut dregs of rice raw material weight ratio after 55 DEG C of hydrolysis temperature:100 add trypsase, under the conditions of pH 8.0
Enzymolysis 30 hours;
C, go out step B resulting material boiling water baths 10min enzyme, and in 4 DEG C, 8000r/min centrifugation 20min are filtered, collected
Supernatant, as walnut protein zymolyte, vacuum freeze drying are preserved.
(2) preparation of trace-element fertilizer
3 parts of magnesium sulfate, 1.5 parts of zinc sulfate, 4 parts of manganese sulfate, 5 parts of ferrous sulfate, 5 parts of borax, sulfuric acid are weighed by weight
2 parts of copper, 1.5 parts of sodium molybdate, 3 parts of silica, 3 parts of Arabic gum, 85 parts of water, 5 parts of walnut protein zymolyte, by alleged raw material
Mixing, stirring is to being completely dissolved, and pH value is adjusted between 5.5~6.5, is produced.
Application Example
A kind of walnut protein zymolyte regulation and control of the present invention prepare the trace-element fertilizer containing nanometer selenium to paddy rice agronomy
The influence of shape, protein and Se content
1st, experimental design
For studying thing for paddy rice, experiment is set between six processing, 3 repetitions, each processing cell altogether certain distance, small
Area's area is 20m2, random district's groups arrangement.Each processing sets as follows:
Processing 1:Any trace-element fertilizer is not applied;
Processing 2:The trace-element fertilizer and water in mass ratio 1 prepared by above-mentioned comparative example 1:500 multiplying power dilution;
Processing 3:The trace-element fertilizer and water in mass ratio 1 prepared by above-mentioned comparative example 2:500 multiplying power dilution;
Processing 4:The trace-element fertilizer and water in mass ratio 1 prepared by above-described embodiment 1:500 multiplying power dilution;
Processing 5:The trace-element fertilizer and water in mass ratio 1 prepared by above-described embodiment 2:500 multiplying power dilution;
Processing 6:The trace-element fertilizer and water in mass ratio 1 prepared by above-described embodiment 3:500 multiplying power dilution.
2nd, fertilization time and method
Fertilization time:Each processing respectively sprays alignment processing dilution fertilizer in rice seedling, heading stage and pustulation period once,
Each amount of spraying is 75 liters/mu, wherein, processing 1 sprays the water of respective amount.
Fertilizing method:In the sunny calm morning, uniformly sprayed in blade surface using sprayer, each processing is molten when spraying
Appropriate spray adjuvantses are added in liquid, solution is improved in blade face residence time and solution viscosity, selenium is applied so as to strengthen
Purpose.Experimental plot others base manure, topdress, irrigate and the condition such as other field management is consistent.
3rd, pilot survey
Before rice harves, determine its economical character plant height, number of productive ear, mass of 1000 kernel and yield, grain protein and
Se content.Sample collection is that 20~30 plants are investigated per cell, is averaged.
4th, result of the test:As a result it is as shown in table 1 below.
Influence of the table 1 to Agronomic Traits in Rice, protein and Se content
5th, conclusion
By the investigation statisticses to Agronomic Traits in Rice, protein and Se content, using a kind of walnut of the present invention
Protein zymolyte regulation and control, which prepare the trace-element fertilizer containing nanometer selenium, significantly improves Agronomic Traits in Rice, grain protein and selenium
Content is significantly improved, and illustrates that the amino acid contained by trace-element fertilizer of the present invention and trace element have very to crop growth
Good facilitation, can significantly improve crop quality, improve crop yield.Wherein, the micro member that prepared by the embodiment of the present invention 2
Fertilizer treatment effect is optimal, compared with applying water, the processing of embodiment 2 make Plant Height of Rice, number of productive ear, mass of 1000 kernel, yield and
Grain protein content increases by 8.44%, 33.58%, 32.76%, 30.08%, 15.62% respectively, seed Se content by
0.013mg/kg increases to 0.273mg/kg, and incrementss are 0.26mg/kg, meets national standard GB/T 22499-2008 selenium-rich rice
To 0.04~0.3mg/kg of definition of selenium-rich rice in paddy.
After preferred embodiment is described in detail, relevant industries technical staff can have good operability, therefore,
Variations and modifications can be carried out in protection scope of the present invention, all technical spirits according to the present invention are carried out to above example
Any simple modification, equivalent variations and modification, belong to the scope of technical solution of the present invention.
Claims (4)
1. a kind of method that walnut protein zymolyte regulation and control prepare the trace-element fertilizer containing nanometer selenium, it is characterised in that including
Following steps:
(1) preparation of walnut protein zymolyte
A, by degreasing walnut dregs of rice raw material decontamination dry for standby;
B, the degreasing walnut dregs of rice raw material of drying is taken in enzymatic vessel, be 1 by solid-liquid ratio:7 add water, mix, are pre-heated to enzymolysis
After 55 DEG C of temperature, trypsase is added, is digested under the conditions of pH 8.0;
C, go out step B resulting material boiling water baths 10min enzyme, and in 4 DEG C, supernatant is collected in 8000r/min centrifugation 20min, filtering
Liquid, as walnut protein zymolyte, vacuum freeze drying are preserved;
(2) preparation of walnut protein zymolyte --- nanometer selenium
A, walnut protein zymolyte freeze-dried powder obtained by step (1) is taken, by solid-liquid ratio 5:1 adds deionized water, stirs to complete
Dissolving, adds 1mmol/L sodium selenite solutions, after whirlpool is mixed, 4mmol/L vitamin Cs (Vc) solution is added dropwise, 45 DEG C of perseverances are placed in
Concussion is incubated 24h in warm shaking table;
B, reaction solution obtained by step A is transferred in the bag filter that molecular cut off is 500u, 4 DEG C of lucifuges dialysis of distilled water, often
First water is changed every 2h;The outer liquid of bag filter is detected through ICP-AES dialyses without stopping after plasma selenium, gained sample in bag filter
It is standby after as walnut protein zymolyte --- nanometer selenium, vacuum freeze drying;
(3) preparation of trace-element fertilizer
1~5 part of magnesium sulfate, 0.5~2.5 part of zinc sulfate, 1~7 part of manganese sulfate, 2~8 parts of ferrous sulfate, boron are weighed by weight
2~8 parts of sand, 1~3 part of copper sulphate, 0.5~2.5 part of sodium molybdate, silica 1~5 part, 1~5 part of Arabic gum, water 80~90
Part, with the walnut protein zymolyte obtained by step (2) --- 2~8 parts of nanometer selenium freeze-dried powder mix, is stirred to being completely dissolved, pH
Value regulation is produced between 5.5~6.5.
2. the walnut protein zymolyte regulation and control according to claim 1 prepare the side of the trace-element fertilizer containing nanometer selenium
Method, it is characterised in that it is 1 that enzyme amount is pressed in the step (1) with degreasing walnut dregs of rice raw material weight ratio:50~200 add tryptose
Enzyme, wherein enzyme activity is in terms of 100000U/g.
3. the walnut protein zymolyte regulation and control according to claim 1 prepare the side of the trace-element fertilizer containing nanometer selenium
Method, it is characterised in that enzymolysis time is 20~40 hours in the step (1).
4. the method for preparing the trace-element fertilizer containing nanometer selenium according to the walnut protein zymolyte regulation and control described in claim 1,
Characterized in that, sodium selenite solution, vitamin C (Vc) solution, walnut protein zymolyte solution press volume in the step (2)
Than for Vc:Na2SeO3:Walnut protein zymolyte solution=4:4:1 ratio is mixed.
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