CN106973860A - Springtail live body Morphologic Characteristics and behavior microscope observation device - Google Patents
Springtail live body Morphologic Characteristics and behavior microscope observation device Download PDFInfo
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- CN106973860A CN106973860A CN201710160373.9A CN201710160373A CN106973860A CN 106973860 A CN106973860 A CN 106973860A CN 201710160373 A CN201710160373 A CN 201710160373A CN 106973860 A CN106973860 A CN 106973860A
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- 241001427559 Collembola Species 0.000 title claims abstract description 90
- 230000000877 morphologic effect Effects 0.000 title claims abstract description 23
- 239000006059 cover glass Substances 0.000 claims abstract description 69
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 58
- 229920001817 Agar Polymers 0.000 claims abstract description 55
- 239000008272 agar Substances 0.000 claims abstract description 55
- 240000005373 Panax quinquefolius Species 0.000 claims abstract description 35
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 35
- 239000003292 glue Substances 0.000 claims abstract description 24
- 230000000694 effects Effects 0.000 claims abstract description 18
- 239000002609 medium Substances 0.000 claims description 42
- 239000000123 paper Substances 0.000 claims description 18
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 14
- 235000013305 food Nutrition 0.000 claims description 9
- 239000011111 cardboard Substances 0.000 claims description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 239000001569 carbon dioxide Substances 0.000 claims description 7
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 7
- 229910052760 oxygen Inorganic materials 0.000 claims description 7
- 239000001301 oxygen Substances 0.000 claims description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 238000004026 adhesive bonding Methods 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 239000002912 waste gas Substances 0.000 claims description 6
- 210000002421 cell wall Anatomy 0.000 claims description 5
- 230000029058 respiratory gaseous exchange Effects 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- 239000013028 medium composition Substances 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 3
- 239000000853 adhesive Substances 0.000 claims description 2
- 230000001070 adhesive effect Effects 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000011087 paperboard Substances 0.000 claims description 2
- 239000008367 deionised water Substances 0.000 claims 1
- 229910021641 deionized water Inorganic materials 0.000 claims 1
- 239000002689 soil Substances 0.000 description 9
- 239000007789 gas Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 210000005239 tubule Anatomy 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 238000006424 Flood reaction Methods 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000500891 Insecta Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001149 cognitive effect Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000002242 deionisation method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000158 ommatidium Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000012173 sealing wax Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Springtail live body Morphologic Characteristics and behavior microscope observation device, belong to technical field of life science, are made up of double-deck papery fixed plate (1) and Sheng worm plate (17), it is characterised in that：Described Sheng worm plate (17) is transparent, point upper, middle and lower-ranking, and upper strata is upper strata cover glass (12), and lower floor is lower floor's cover glass (13), and middle one layer is yeast agar medium layer (11)；The confession live body springtail activity space that yeast agar medium layer (11) is surrounded by air guide capillary (5), yeast agar medium layer (11) and by yeast agar medium is contained worm chamber (10) and constituted, and fixing glue (14) will contain worm plate (17) and be bonded into an entirety.Springtail live body Morphologic Characteristics and behavior microscope make simple with observation device, and workable, with low cost, effect is obvious.
Description
Technical field
The present invention relates to a kind of springtail live body Morphologic Characteristics and behavior microscope observation device, belong to life science
Technical field.
Background technology
Springtail (Collembola) is under the jurisdiction of Arthropoda Insecta Apterigota, external springtail classification on taxology
Learn and existing more than the 100 years history of Phylogenetic Studies, the world has reported 8092 kinds so far.Springtail body is small, into more than polypide length
1 millimeter；Widely distributed, every place for having soil can almost find the figure of springtail, and springtail is in soil ecosystem
In play very important effect.Their predations by taking food and with other soil animals each other, participate in soil material
Cyclic process and During Leaf Litter Decomposition, can improve the fertility of soil；By promoting soil microaggregate to be formed, to safeguard soil
Physicochemical property and edaphone；The animals such as plant, hypha,hyphae or nematode by taking food soil rot, suppress to plant
The pest and disease damage of thing；In addition can also be by reducing the toxicity of heavy metal and organic pollution come repairing polluted soil.At present, to springtail
Research more rest on stage of typoiogical classification, classification feature used is mainly the quantity of ommatidium and relatively large on springtail body
The hair of small, postantennal organ shape and length, the shape of feeler and structure, the structure of mouthpart each several part and each body segment of feature, body
The common 30- such as sequence and structure, the structure and feature of 3 pairs of appendages, the structure of ereisma each several part and its feature, the design feature of gential plates
32.However, these features of springtail have it is highly stable, have is then variable, once these stable features have difference,
The foundation for being accredited as novel species just can be used as according to some or several features.It is considerably less for the otherwise research of springtail,
Reason one is that springtail polypide is very small, body length only 1 millimeter；Two be the less of forefathers' further investigation, because many no ones dabble,
Plus the limitation of instrument and equipment, current people are caused to understand springtail considerably less.Springtail is an ancient monoid, fossil evidence
Show Collembola springtail：Rhyniella praecursor are one of insects earliest on the earth, are begun now in about 400,000,000 years away from the present
The devonian period is in early days to mid-term.It is not cognitive secret to have certainly in very long historical floods, with springtail many.How to obtain
Obtain these secretSee them clearly firstly the need of seeing, what monoid springtail observed by can telling probably belongs to, then,
Observed and recorded its habits and customs, behavioural characteristic, therefrom find the knowledge that some can be used in the mankind.But, the corpusculum of springtail
Type, spring property are must not beat, must not touch, and them can be fixed by being placed in alcohol or formaldehyde, but can not observe each feature such as
Where played a role on live body；It is placed in slide or culture dish and thinks observation, but it is uncontrolled, just to see them, moment is just
Do not know where jump.Therefore, design one how using the confined space by live body springtail be limited in one be available in it is micro-
Carry out observing under mirror and do not influence the device of its activity and behavior to turn into a great problem for being badly in need of solution, so using containing worm plate,
Live body springtail is limited in can observe between the double-deck cover glass used under the microscope；Blocked with yeast agar medium layer
Firmly between double-deck cover glass cavity periphery, provide food and moisture while being grown for springtail；With the air guide hair communicated with the external world
Tubule grows the oxygen needed for providing for springtail, discharges the waste gas such as the carbon dioxide of itself release；Will with double-deck papery fixed plate
Contain worm plate and fix protection, so as to operational observations under the microscope, invent a kind of springtail live body Morphologic Characteristics and behavior is micro-
Mirror is necessary with observation device.
The content of the invention
In order to overcome how using the confined space by live body springtail be limited in one be available for being observed under the microscope and
The problem of the device of its activity and behavior is not influenceed, is used the invention provides springtail live body Morphologic Characteristics and behavior microscope
Device is observed, this kind of springtail live body Morphologic Characteristics and behavior microscope observation device are using worm plate is contained, by live body springtail
Being limited in can observe between the double-deck cover glass used under the microscope；Double layer lid glass is blocked with yeast agar medium layer
The periphery of cavity between piece, while providing food and moisture for springtail growth；It is springtail with the air guide capillary communicated with the external world
Oxygen needed for offer is provided, the waste gas such as the carbon dioxide of itself release are discharged；Fixed with double-deck papery fixed plate by worm plate is contained
Protection, so as to operational observations under the microscope, reaches that live body springtail just can be limited in one using the confined space is available in
Carry out observing and not influenceing the purpose of the device of its activity and behavior under microscope.
The technical solution adopted for the present invention to solve the technical problems is：
Springtail live body Morphologic Characteristics and behavior microscope of the present invention observation device, by the double-deck He of papery fixed plate 1
Worm plate 17 is contained to constitute, it is characterised in that：Described Sheng worm plate 17 is transparent, point upper, middle and lower-ranking, and upper strata is upper strata cover glass 12,
It is that length is 18 millimeters, width is 18 millimeters, and thickness is 0.17 millimeter of commercially available cover glass；Lower floor is lower floor's cover glass 13, is
Length is 18 millimeters, and width is 18 millimeters, and thickness is 0.17 millimeter of commercially available cover glass；Middle one layer is yeast agar medium
Layer 11；Yeast agar medium layer 11 is surrounded by air guide capillary 5, yeast agar medium layer 11 and by yeast agar medium
Confession live body springtail activity space contain worm chamber 10 and constitute, fixing glue 14 is bonded into an entirety by worm plate 17 is contained.
The described cuboid of Sheng worm chamber 10, length is 13 millimeters, and width is 13 millimeters, is highly 0.8-1.8 millimeters, is
The confession live body springtail activity space surrounded by yeast agar medium, during with micro- sem observation, mainly observation is contained in worm chamber 10
The springtail of activity；The yeast agar medium for containing the surrounding filling of worm chamber 10 provides food yeast and moisture, air guide capillary for springtail
Pipe 5 provides the oxygen of breathing for springtail, and discharge is metabolized the waste gas such as the carbon dioxide of generation；Described air guide capillary 5 totally 4
Root, 2 is respectively placed in the double-deck both sides direction of papery fixed plate 1, every air guide capillary 5 is the nature of glass, cross circular section is annular,
Interior diameter is 0.18-0.6 millimeters, and overall diameter is 0.8-1.8 millimeters, and length is 6.5 millimeters, and lead to Sheng worm chamber 10 for one end, another
The external world of the double-deck both side edges of papery fixed plate 1 is led at end；Described yeast agar medium layer 11 is led by containing worm chamber 10 and being placed with
The yeast agar medium composition of gas capillary 5, positioned at middle position on the above-below direction of worm plate 17 is contained, described yeast agar is trained
The periphery that base is located between upper strata cover glass 12 and lower floor's cover glass 13 is supported, width is 5 millimeters, and thickness is 0.8-1.8 millimeters, ferment
The formula of female agar medium is：10 grams of sodium chloride, 10 grams of peptone, 3 grams of beef extract, 5 grams of dusty yeast, 15 grams of agar, plus go
Ionized water adjusts pH value to 7.0-7.2 to 1 liter, standby after packing sterilizing.
Described double-deck papery fixed plate 1 is fixed to contain worm plate 17, be pasted with label paper 3 and be easy to observe jump under the microscope
The cuboid structure of worm live body, papery, length is 76 millimeters, and width is 26 millimeters, and thickness is 3-5 millimeters, one end upper surface
Label paper 3 is stained with, other end center has the space in the middle of hole, the hole to be that length is 13 millimeters, width is 13 millimeters, depth is
3-5 millimeters of observation vallecular cavity 7, the wall of the observation surrounding of vallecular cavity 7 is observation cell wall 6；Two layers, two about double-deck 1 point of papery fixed plate
It is combined between layer by gluing, double-deck papery fixed plate bonding seam 2 is formed in periphery；Double-deck papery fixed plate bonding seam 2
It is that mounted Sheng worm plate 17 is put into the Sheng worm board slot 4 between two layers of cardboard in the middle of the double-deck periphery of papery fixed plate 1
Afterwards, by it is gluing altogether, by contain worm plate 17 fix, for ease of observation in the middle of the double-deck periphery of papery fixed plate 1 formed
One fill up glue seam；Label paper 3 is commercially available adhesive sticker, and length is 26 millimeters, and width is 26 millimeters, sticks to duplex paper
The upper surface of the one end of matter fixed plate 1；Contain worm board slot 4 and be located at observation vallecular cavity 7 above-below direction middle position, length is 20 millimeters, wide
Spend for 20 millimeters, thickness is 1.14-2.14 millimeters, be that the fixed double-deck papery fixed plate 1 for containing worm plate 17 is installed to contain worm plate 17
The space that upper and lower faces are surrounded by the cardboard that thickness is 0.53-0.615 millimeters, the center section of observation vallecular cavity 7 is exactly to contain worm plate
17 middle bodies；It is upper, middle, and lower part to observe vallecular cavity 7 and contain 17 points of worm plate, and two parts are all empty up and down, referred to as contain worm plate
Epicoele 9, length is 13 millimeters, and width is 13 millimeters, is highly 0.53-0.615 millimeters, surrounds the perisporium of Sheng worm plate epicoele 9 four and is
Cavity wall 8 on worm plate is contained, the height for containing cavity wall 8 on worm plate is 0.53-0.615 millimeters, and length and width is 13 millimeters.
Described springtail live body Morphologic Characteristics and behavior microscope are as follows with observation device manufacturing process：Paper is used first
Plate makes double-deck papery fixed plate 1 and air guide capillary 5, a cover glass is then taken as lower floor's cover glass 13, in lower floor's lid
The yeast agar medium of solid state is spread on the upper surface periphery of slide 13, is put into air guide capillary 5, then, by live body springtail
It is put into the space that yeast agar medium is surrounded, covers another cover glass, the cover glass is upper strata cover glass 12, institute's shape
Into the space of storage springtail be referred to as containing worm chamber 10, contain worm chamber 10 and be placed with the yeast agar medium of air guide capillary 5 and formed
Yeast agar medium layer 11, light pressure upper strata cover glass 12, by the lower surface of lower floor's cover glass 13 and the upper surface of upper strata cover glass 12
The distance between be adjusted to 1.14-2.14 millimeters, then, upper strata cover glass 12 and lower floor's cover glass 13 are applied to fixing glue 14
Periphery, by upper strata cover glass 12 together with the good bond of lower floor cover glass 13, you can Sheng worm plate 17, air guide capillary is made
5 leave 4 millimeters of length containing outside the both sides of worm plate 17.Then, Sheng worm plate 17 is placed on to the Sheng worm plate of double-deck papery fixed plate 1
Two layers of cardboard of double-deck papery fixed plate 1, is glued together by groove 4 with glue, in the double-deck papery fixed plate of the observation opposite side of vallecular cavity 7
Labelled paper 3 is glued in 1 outer surface, is finally made springtail live body Morphologic Characteristics and behavior microscope observation device
Beneficial effects of the present invention are that springtail live body Morphologic Characteristics and behavior microscope observation device utilize and contain worm
Plate, live body springtail is limited in can observe between the double-deck cover glass used under the microscope；With yeast agar medium layer
The periphery of cavity between double-deck cover glass is blocked, while providing food and moisture for springtail growth；With with the external world communicate lead
Gas capillary grows the oxygen needed for providing for springtail, discharges the waste gas such as the carbon dioxide of itself release；Fixed with double-deck papery
Plate will contain worm plate and fix protection, and so as to operational observations under the microscope, can just live body springtail be limited using the confined space by reaching
System is available for carrying out observing under the microscope and not influenceed the purpose of the device of its activity and behavior at one.Springtail live body form knot
Structure feature and behavior microscope make simple with observation device, and workable, with low cost, effect is obvious.
Brief description of the drawings
The present invention is further described below in conjunction with the accompanying drawings.
Fig. 1 is the overall structure signal of springtail live body Morphologic Characteristics of the present invention and behavior microscope observation device
Figure.
Fig. 2 is the Sheng worm plate overall structure that springtail live body Morphologic Characteristics of the present invention and behavior microscope observe device
Schematic diagram.
1. double-deck papery fixed plate in figure, 2. double-deck papery fixed plate bonding seams, 3. label papers, 4. contain worm board slot, and 5. lead
Gas capillary, 6. observation cell walls, 7. observation vallecular cavities, 8. contain cavity wall on worm plate, and 9. contain worm plate epicoele, and 10. contain worm chamber, 11. yeast
Agar medium layer, 12. upper strata cover glasses, 13. lower floor's cover glasses, 14. fixing glues, 15. fixing glue edge, 16. air guide capillaries
Receiving hole, 17. contain worm plate.
Embodiment
Embodiment one：
As illustrated, springtail live body Morphologic Characteristics of the present invention and behavior microscope observation device are consolidated by double-deck papery
Fixed board 1, double-deck papery fixed plate bonding seam 2, label paper 3, Sheng worm board slot 4, air guide capillary 5, observation cell wall 6, observation vallecular cavity
7th, cavity wall 8 on worm plate is contained, worm plate epicoele 9 is contained, contains worm chamber 10, yeast agar medium layer 11, upper strata cover glass 12, lower floor's lid glass
Piece 13, fixing glue 14, fixing glue edge 15, air guide capillary receiving hole 16, Sheng worm plate 17 are constituted.Double-deck papery fixed plate 1 is solid
Surely contain worm plate 17, be stained with the cuboid structure that label paper 3 is easy to observe springtail live body under the microscope, papery, length is 76
Millimeter, width is 26 millimeters, and thickness is 3-5 millimeters, and one end upper surface is stained with label paper 3, and other end center has in hole, the hole
Between space be that length is 13 millimeters, width is 13 millimeters, and depth is 3-5 millimeter of observation vallecular cavity 7, observes the surrounding of vallecular cavity 7
Wall is observation cell wall 6；It is combined about double-deck 1 point of papery fixed plate between two layers, two layers by gluing, forms double in periphery
Layer papery fixed plate bonding seam 2.Double-deck papery fixed plate bonding seam 2 is located in the middle of the double-deck periphery of papery fixed plate 1, is two layers of paper
Be put into Sheng worm board slot 4 between plate after mounted Sheng worm plate 17, by it is gluing altogether, worm plate 17 will be contained and fix, be
The seam that one for being easy to observe and being formed in the middle of the double-deck periphery of papery fixed plate 1 fills up glue.Label paper 3 is commercially available not do
Glue, length is 26 millimeters, and width is 26 millimeters, sticks to the upper surface of double-deck one end of papery fixed plate 1, to be seen to mark
The relevant information of live body springtail sample.Contain worm board slot 4 and be located at observation vallecular cavity 7 above-below direction middle position, length is 20 millimeters, wide
Spend for 20 millimeters, thickness is 1.14-2.14 millimeters, be that the fixed double-deck papery fixed plate 1 for containing worm plate 17 is installed to contain worm plate 17
The space that upper and lower faces are surrounded by the cardboard that thickness is 0.53-0.615 millimeters, the center section of observation vallecular cavity 7 is exactly to contain worm plate
17 middle bodies, for observing live body springtail.Contain worm board slot 4 to install after fixed Sheng worm plate 17, observation vallecular cavity 7 is just contained worm plate 17
It is divided into upper, middle, and lower part, two parts are all empty up and down, referred to as contains worm plate epicoele 9, length is 13 millimeters, width is 13 millis
Rice, is highly 0.53-0.615 millimeters, surrounds the perisporium of Sheng worm plate epicoele 9 four to contain cavity wall 8 on worm plate, contains cavity wall 8 on worm plate
It it is highly 0.53-0.615 millimeters, length and width is 13 millimeters.It is to hold live body springtail to contain worm plate 17, allows springtail in it
Activity, and be the movable transparent configuration for being available for observing under the microscope that food, moisture and air are provided of springtail, point upper, middle and lower
Three layers, upper strata is upper strata cover glass 12, is that length is 18 millimeters, width is 18 millimeters, and thickness is 0.17 millimeter of commercially available lid glass
Piece；Lower floor is lower floor's cover glass 13, is that length is 18 millimeters, width is 18 millimeters, and thickness is 0.17 millimeter of commercially available cover glass；
Middle one layer is yeast agar medium layer 11；Yeast agar medium layer 11 is by air guide capillary 5, yeast agar medium layer
11 and the confession live body springtail activity space that is surrounded by yeast agar medium be referred to as containing worm chamber 10 and constitute, fixing glue 14 will contain worm plate
17 are bonded into an entirety.The cuboid of worm chamber 10 is contained, length is 13 millimeters, and width is 13 millimeters, be highly 0.8-1.8 millis
Rice, is the confession live body springtail activity space surrounded by yeast agar medium, during with micro- sem observation, and worm chamber is contained in mainly observation
Movable springtail in 10, because springtail body length is generally 1 millimeter, bullet is hit exactly in the ventrite of body belly first
Device, height that the length of ereisma is left ground and supported plus thickness, the 3 pairs of appendages support bodies of springtail body and
The length of body surface bristle, four height added up are almost equal with body length so that springtail is limited in Sheng worm chamber 10, can
Activity, but can not takeoff, contains can also being close to body surface of individual slightly larger point worm chamber inwall about 10, it is easier to seen
Examine the topographic features of its body surface；The yeast agar medium for containing the surrounding filling of worm chamber 10 provides food yeast and water for springtail
Point, air guide capillary 5 provides the oxygen of breathing for springtail, and discharge is metabolized the waste gas such as the carbon dioxide of generation, so as to ensure that
Springtail lives less impacted and is easy to observe its life habit and Behavior character in a short time.Air guide capillary 5 totally 4
Root, 2 is respectively placed in the double-deck both sides direction of papery fixed plate 1, every air guide capillary 5 is the nature of glass, cross circular section is annular,
Interior diameter is 0.18-0.6 millimeters, and overall diameter is 0.8-1.8 millimeters, and length is 6.5 millimeters, and lead to Sheng worm chamber 10 for one end, another
The external world of the double-deck both side edges of papery fixed plate 1 is led at end, is to provide breathing use to move in the observed springtail contained in worm chamber 10
Oxygen, the exhaust steam passage such as carbon dioxide that discharge metabolism is produced, meanwhile, its interior diameter is less than springtail active state lower body
Highly, it is to avoid springtail escapes out from air guide capillary 5.Yeast agar medium layer 11 is by containing worm chamber 10 and being placed with air guide hair
The yeast agar medium composition of tubule 5, middle position, described yeast agar medium on the Sheng above-below direction of worm plate 17
Periphery between upper strata cover glass 12 and lower floor's cover glass 13, width is 5 millimeters, and thickness is 0.8-1.8 millimeters, yeast fine jade
The formula of fat culture medium is：10 grams of sodium chloride, 10 grams of peptone, 3 grams of beef extract, 5 grams of dusty yeast, 15 grams of agar, plus deionization
Water adjusts pH value to 7.0-7.2 to 1 liter, standby after packing sterilizing.Fixing glue 14 is can be quickly by upper strata cover glass 12 and lower floor
Cover glass 13 is bonded together from periphery, the glue for having certain degree of hardness after bonding, such as nail polish, sealing wax etc..Upper strata cover glass 12
With the periphery of lower floor cover glass 13 by after the sealing of fixing glue 14, part glue is containing the outer surface peripheral region formation one fixed width of worm plate 17
Glued membrane, the inner edge of glued membrane is fixing glue edge 15, when making Sheng worm plate 17, and fixing glue edge 15 is covered from upper strata cover glass 12 or lower floor
The distance at the edge of slide 13 is 1-3 millimeters, is less than 5 millimeters, in case the width more than yeast agar medium layer 11 reaches Sheng
The outer influence observation of worm chamber 10, is contained outside worm chambers 10 if having exceeded 5 millimeters when making and having reached, can with sharp blade by more than
Cross out part.The space that air guide capillary 5 is held in yeast agar medium layer 11 is air guide capillary receiving hole 16, is to make
When place the hole formed after air guide capillary 5.Springtail live body Morphologic Characteristics and behavior microscope made of observation device
Journey is as follows：First with the double-deck papery fixed plate 1 of paperboard and air guide capillary 5, a cover glass is then taken to be covered as lower floor
Slide 13, spreads the yeast agar medium of solid state on the upper surface periphery of lower floor's cover glass 13, is put into air guide capillary 5,
Then, live body springtail is put into the space that yeast agar medium is surrounded, covers another cover glass, on the cover glass is
Layer cover glass 12, the space of the storage springtail formed is referred to as containing worm chamber 10, contains worm chamber 10 and is placed with the yeast of air guide capillary 5
Agar medium formation yeast agar medium layer 11, light pressure upper strata cover glass 12, by the lower surface of lower floor's cover glass 13 and upper strata
The distance between upper surface of cover glass 12 is adjusted to 1.14-2.14 millimeters, then, and upper strata cover glass 12 is applied to fixing glue 14
With the periphery of lower floor cover glass 13, by upper strata cover glass 12 together with the good bond of lower floor cover glass 13, you can Sheng worm is made
Plate 17, air guide capillary 5 leaves 4 millimeters of length containing outside the both sides of worm plate 17.Then, worm plate 17 will be contained and is placed on double-deck papery
Two layers of cardboard of double-deck papery fixed plate 1, is glued together by the Sheng worm board slot 4 of fixed plate 1 with glue, in observation vallecular cavity 7 relative one
Labelled paper 3 is glued in the outer surface of the double-deck papery fixed plate 1 in end, is finally made springtail live body Morphologic Characteristics and behavior is micro-
Mirror observation device.
The general principle and principal character and advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it will be appreciated that the present invention is not limited to the above embodiments, and described in above-described embodiment and specification is to illustrate this hair
Bright principle, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
It all fall within the protetion scope of the claimed invention with improvement, its is equivalent by appended claims for the claimed scope of the invention
Thing is defined.
Claims (4)
1. springtail live body Morphologic Characteristics and behavior microscope observation device, by double-deck papery fixed plate (1) and Sheng worm plate
(17) constitute, it is characterised in that：Described Sheng worm plate (17) is transparent, point upper, middle and lower-ranking, and upper strata is upper strata cover glass (12),
It is that length is 18 millimeters, width is 18 millimeters, and thickness is 0.17 millimeter of commercially available cover glass；Lower floor is lower floor's cover glass (13),
It is that length is 18 millimeters, width is 18 millimeters, and thickness is 0.17 millimeter of commercially available cover glass；Middle one layer is yeast agar culture
Basic unit (11)；Yeast agar medium layer (11) is by air guide capillary (5), yeast agar medium layer (11) and by yeast agar
The confession live body springtail activity space that culture medium is surrounded is contained worm chamber (10) and constituted, and fixing glue (14) will contain worm plate (17) and be bonded into one
It is overall.
2. springtail live body Morphologic Characteristics according to claim 1 and behavior microscope observation device, its feature exist
In：Described Sheng worm chamber (10) cuboid, length be 13 millimeters, width be 13 millimeters, be highly 0.8-1.8 millimeters, be by
The confession live body springtail activity space that yeast agar medium is surrounded, during with micro- sem observation, mainly observation is contained in worm chamber (10)
The springtail of activity；The yeast agar medium for containing filling around worm chamber (10) provides food (yeast) and moisture, air guide for springtail
Capillary (5) provides the oxygen of breathing for springtail, and discharge is metabolized the waste gas such as the carbon dioxide of generation；Described air guide capillary
(5) totally 4,2 are respectively placed in double-deck papery fixed plate (1) both sides direction, every air guide capillary (5) is the nature of glass, it is horizontal
Section annular, interior diameter is 0.18-0.6 millimeters, and overall diameter is 0.8-1.8 millimeters, and length is 6.5 millimeters, and Sheng is led in one end
Worm chamber (10), the other end leads to the external world of double-deck papery fixed plate (1) both side edges；Described yeast agar medium layer (11) by
Contain worm chamber (10) and be placed with the yeast agar medium composition of air guide capillary (5), in containing on worm plate (17) above-below direction
Position is entreated, described yeast agar medium is located at the periphery between upper strata cover glass (12) and lower floor's cover glass (13), width
For 5 millimeters, thickness is 0.8-1.8 millimeters, and the formula of yeast agar medium is：10 grams of sodium chloride, 10 grams of peptone, beef extract
3 grams, 5 grams of dusty yeast, 15 grams of agar, plus deionized water is to 1 liter, adjusts pH value to 7.0-7.2, it is standby after packing sterilizing.
3. springtail live body Morphologic Characteristics according to claim 1 and behavior microscope observation device, its feature exist
In：Described double-deck papery fixed plate (1) is fixed to contain worm plate (17), be pasted with label paper (3) and be easy to observe under the microscope
The cuboid structure of springtail live body, papery, length is 76 millimeters, and width is 26 millimeters, and thickness is 3-5 millimeters, one end upper table
Face is stained with label paper (3), and other end center has the space in the middle of hole, the hole to be that length is 13 millimeters, and width is 13 millimeters, deep
The observation vallecular cavity (7) for 3-5 millimeters is spent, the wall of observation vallecular cavity (7) surrounding is observation cell wall (6)；Double-deck papery fixed plate (1) point
It is combined between two layers up and down, two layers by gluing, double-deck papery fixed plate bonding seam (2) is formed in periphery；Double-deck papery
Fixed plate bonding seam (2) is located in the middle of double-deck papery fixed plate (1) periphery, is put in the Sheng worm board slot (4) between two layers of cardboard
Enter after mounted Sheng worm plate (17), by it is gluing altogether, will contain worm plate (17) fix, for ease of observation in duplex paper
The seam that one formed in the middle of matter fixed plate (1) periphery fills up glue；Label paper (3) is commercially available adhesive sticker, and length is 26 millimeters,
Width is 26 millimeters, sticks to the upper surface of double-deck papery fixed plate (1) one end；Worm board slot (4) is contained to be located on observation vallecular cavity (7)
Lower direction middle position, length is 20 millimeters, and width is 20 millimeters, and thickness is 1.14-2.14 millimeters, is to install to fix to contain worm plate
(17) double-deck papery fixed plate (1) is surrounded in Sheng worm plate (17) upper and lower faces by thickness for 0.53-0.615 millimeters of cardboard
Space, observation vallecular cavity (7) center section be exactly to contain worm plate (17) middle body；Observation vallecular cavity (7) is contained worm plate (17) and is divided into
Upper, middle, and lower part, two parts are all empty up and down, referred to as contain worm plate epicoele (9), and length is 13 millimeters, and width is 13 millis
Rice, is highly 0.53-0.615 millimeters, surrounds the perisporium of Sheng worm plate epicoele (9) four to contain cavity wall (8) on worm plate, contains cavity wall on worm plate
(8) height is 0.53-0.615 millimeters, and length and width is 13 millimeters.
4. springtail live body Morphologic Characteristics according to claim 1 and behavior microscope observation device, its feature exist
In：Described springtail live body Morphologic Characteristics and behavior microscope are as follows with observation device manufacturing process：Cardboard system is used first
Make double-deck papery fixed plate (1) and air guide capillary (5), a cover glass is then taken as lower floor's cover glass (13), in lower floor
The yeast agar medium of solid state is spread on cover glass (13) upper surface periphery, is put into air guide capillary (5), then, by work
Body springtail is put into the space that yeast agar medium is surrounded, and covers another cover glass, and the cover glass is upper strata cover glass
(12) space of the storage springtail, formed is referred to as containing worm chamber (10), contains worm chamber (10) and is placed with the yeast of air guide capillary (5)
Agar medium formation yeast agar medium layer (11), light pressure upper strata cover glass (12), by lower floor's cover glass (13) lower surface
1.14-2.14 millimeters are adjusted to the distance between upper strata cover glass (12) upper surface, then, is applied to fixing glue (14)
The periphery of layer cover glass (12) and lower floor's cover glass (13), upper strata cover glass (12) and lower floor's cover glass (13) good bond are existed
Together, you can Sheng worm plate (17) is made, air guide capillary (5) leaves 4 millimeters of length containing outside worm plate (17) both sides；So
Afterwards, worm plate (17) will be contained and is placed on the Sheng worm board slot (4) of double-deck papery fixed plate (1), with glue by the two of double-deck papery fixed plate (1)
Layer paperboard is glued together, and labelled paper (3) is glued in the outer surface of the double-deck papery fixed plate (1) of observation vallecular cavity (7) opposite side,
It is finally made springtail live body Morphologic Characteristics and behavior microscope observation device.
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| CN201710160373.9A CN106973860A (en) | 2017-03-01 | 2017-03-01 | Springtail live body Morphologic Characteristics and behavior microscope observation device |
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| CN201710160373.9A CN106973860A (en) | 2017-03-01 | 2017-03-01 | Springtail live body Morphologic Characteristics and behavior microscope observation device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110286025A (en) * | 2019-07-11 | 2019-09-27 | 云南大学 | A kind of small feeler of insect and the fixed device of brain posture various dimensions and preparation method thereof |
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