CN106970075B - A kind of o-phthalaldehyde Concentration Testing test paper and preparation method thereof - Google Patents
A kind of o-phthalaldehyde Concentration Testing test paper and preparation method thereof Download PDFInfo
- Publication number
- CN106970075B CN106970075B CN201710166963.2A CN201710166963A CN106970075B CN 106970075 B CN106970075 B CN 106970075B CN 201710166963 A CN201710166963 A CN 201710166963A CN 106970075 B CN106970075 B CN 106970075B
- Authority
- CN
- China
- Prior art keywords
- filter paper
- test paper
- phthalaldehyde
- paper
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The present invention relates to a kind of o-phthalaldehyde Concentration Testing test paper and preparation method thereof.The preparation method of the test paper is divided into mother liquor, filter paper pretreatment and filter paper soaking and dyeing three parts.The colour developing principle of the filter paper are as follows: after filter paper and o-phthalaldehyde solution Rapid contact, a certain amount of solution of Adsorption of Filter Paper, if O-phthalic aldehyde concentration is not up to specified threshold value (0.35%), the sodium bisulfite salts on filter paper consume all o-phthalaldehyde being adsorbed on filter paper and its true qualities (light blue) are only presented；If O-phthalic aldehyde concentration reaches specified threshold value, sodium bisulfite salts on filter paper consume part o-phthalaldehyde on filter paper, remaining o-phthalaldehyde is rapidly reacted under the action of developing the color promotor (diammonium hydrogen phosphate) with color developing agent on test paper (urea) shows yellow, then is superimposed the true qualities (light blue) of test paper afterwards in yellow green.The test paper detection method is easy to use, as a result accurately and reliably.
Description
Technical field
The present invention relates to field of chemical detection, and in particular to a kind of to carry out Concentration Testing to the thimerosal containing o-phthalaldehyde
Test paper and preparation method thereof.
Background technique
O-phthalaldehyde is as a kind of novel medical disinfectant, and for the effect for guaranteeing disinfection, concentration need to be greater than
0.35%.The method of detection O-phthalic aldehyde mainly has chemical titration, ultraviolet spectrophotometer method and efficient liquid at present
Phase analysis etc..But the disadvantages of these types of method is all there is cumbersome complexity, and the time is longer, is unsuitable for medical worker's disinfection
Monitoring uses at any time in the process.
O-phthalaldehyde concentration test card has entered researcher's as a kind of method that can directly, rapidly measure concentration
The visual field.As Chinese patent CN 201310220855.0 (a kind of development and production of the quick test paper of aldehyde disinfectant concentration),
Chinese patent CN201511015840.6 (a kind of O-phthalaldehyde disinfectant concentration range Test paper), Chinese patent
CN201510058900.6 (a kind of o-phthalaldehyde thimerosal Concentration Testing test paper) has studied concentration test card.But
It is that they have some limitations, a kind of such as entitled substantive examination of O-phthalaldehyde disinfectant concentration range Test paper is special
Benefit is related to the modification of paper, and complicated operation；A kind of development of entitled quick test paper of aldehyde disinfectant concentration and
Detection time needed for the substantive examination patent of production is 5-8 minutes, and the time is more long.
Summary of the invention
In order to solve the problems, such as described in background technique, the present invention provides a kind of O-phthalic aldehyde concentration quick detection test papers
And the colour developing promotor on paper.Under the action of developing the color promotor, the test paper can according to the concentration of o-phthalaldehyde rapidly,
It delicately shows different degrees of color change, and then reflects the concentration of o-phthalaldehyde.The test paper detection method uses simple
It is convenient, as a result accurately and reliably.
To reach said effect, the present invention adopts the following technical scheme:
A kind of preparation method of O-phthalic aldehyde concentration quick detection test paper, comprising the following steps: (1) preparation of mother liquor:
Dissolution in deionized water is added in sodium hydrogensulfite, methylene blue, color developing agent and colour developing promotor and completely, it is uniform to obtain color
Mother liquor；(2) ordinary filter paper: being cut into the small cube of 1cm × 1cm by the pretreatment of filter paper, be successively immersed in respectively ethyl alcohol, go from
A period of time, is dried for standby after taking-up in sub- water；(3) preparation of o-phthalaldehyde Concentration Testing test paper: by treated in (2)
Filter paper is added in (1) in mother liquor, and after adding a certain amount of ethyl alcohol, ultrasonic vibration for a period of time, takes out filter paper, and drying obtains face
The uniform target filter paper of color；(4) filter paper prepared in (3) is fixed on the substrate not absorbed water with double-sided adhesive, is prepared required
Target test paper.
Preferably, the concentration of sodium bisulphite is 6g/L, and methylene blue concentration is 0.04g/L, and chromogenic agent is
60-160g/L, colour developing promoter concentration are 200-300g/L；
Step (2) filter paper soaking time in ethyl alcohol, deionized water is 20-40min；Drying temperature is 30-50 DEG C；
The ethyl alcohol volumetric concentration that step (3) is added is the 4%-8% of mother liquor volume；The ultrasonic vibration time is 30min, drying
Temperature is 30 DEG C -50 DEG C.
A kind of O-phthalic aldehyde concentration quick detection test paper, is prepared by the above method.
The principle of the present invention is changed using color change display density.
It is specific as follows: the bisulfite when O-phthalic aldehyde concentration is not up to specified threshold value (0.35%), on filter paper
Sodium salt consume it is all be adsorbed in o-phthalaldehyde on filter paper (o-phthalaldehyde preferentially with sodium hydrogensulfite reactant salt, then with it is aobvious
Toner reaction), reactional equation is as follows:
C6H4(CHO)2+2NaHSO3→C6H4(CHOHSO3Na)2
Product shows colourless.Since the true qualities of the test paper are light blue, so filter paper is still light blue color when detection；Work as neighbour
When phthalaldehyde concentration reaches specified threshold value (0.35%), the sodium bisulfite salts on filter paper consume partial adsorbates in filter paper
Upper o-phthalaldehyde, i.e. o-phthalaldehyde have residue.At this point, color developing agent (urea) is in colour developing promotor (phosphoric acid hydrogen two on test paper
Ammonium) under the action of rapidly reacted with o-phthalaldehyde remaining on filter paper and show yellow, then be superimposed test paper true qualities it is (light blue
Color) it is afterwards in yellow green.Wherein, the colour developing principle of color developing agent is the imines chemical combination generated based on urea and O-phthalic aldehyde reaction
Object be in yellow, reaction equation such as following formula:
NH2CONH2+C6H4(CHO)2→C6H4(CNH)2CO+2H2O
On the other hand, to determine effect of colour developing promotor during o-phthalaldehyde test paper color development, blank pair has been done
According to test, i.e., colour developing promotor will be added to be inserted into concentration 0.5% with the Test paper that colour developing promotor production is not added under the same terms
O-phthalaldehyde solution in, compare color change when the time, discovery addition colour developing promotor filter paper developing time about 90s,
The filter paper developing time for not adding colour developing promotor is about 5-6min.This comparative test demonstrates colour developing promotor in O-phthalic
The effect for accelerating chromogenic reaction is played in aldehyde process color, and effect is extremely obvious.
The present invention has the following advantages and beneficial effects:
(1) color change of the invention shows obvious (from the light blue variation to yellow green)；
(2) Coloring Time of the invention is short (90s can develop the color)；
(3) operation of the present invention it is simple, conveniently, it is sensitive.
Detailed description of the invention
Fig. 1 o-phthalaldehyde Test paper detects various concentration o-phthalaldehyde solution colour developing figure
From left to right: O-phthalic aldehyde concentration is followed successively by 0.1%, 0.2%, 0.3%, 0.4%, 0.5%.
Specific embodiment
By following detailed description combination attached drawing it will be further appreciated that the features and advantages of the invention.Provided implementation
Example is only the explanation to the method for the present invention, remaining content without limiting the invention in any way announcement.
Embodiment 1
0.3g sodium hydrogensulfite, 5g urea and 10g diammonium hydrogen phosphate, 5mL 0.4g/L aqueous solution of methylene blue are added
It is dissolved completely in 45mL deionized water, obtains the uniform mother liquor of color；Ordinary filter paper is cut into the small cube of 1cm × 1cm, point
It is not immersed in ethyl alcohol successively, 30min in deionized water, is dried for standby for 50 DEG C after taking-up；It (3) will be in the addition of pretreated filter paper
In mother liquor, after adding the ethyl alcohol of 2mL, ultrasonic vibration 30min takes out filter paper, and drying obtains the uniform target of color at 30 DEG C
Filter paper.
Prepared filter paper is fixed on the substrate not absorbed water with double-sided adhesive, prepares required target test paper.
The filter paper fixed is inserted into 3s in surveyed solution, it is light to dry after taking out, start timing, reads face after 90s
Color.
Fig. 1 is respectively O-phthalic aldehyde concentration when being 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, and test paper is aobvious after 90s
Pornographic condition.As can be seen that concentration is 0.1%, filter paper is still in primary colors (light blue) when 0.2%, and 0.3% when starts to change colour, but
It is that color is shallower also uneven, yellow green is obvious when concentration reaches 0.4% and color is uniform.It can be concluded that the filter paper is to adjacent benzene
The detection speed of dicarbaldehyde is fast, effect is good.
Embodiment 2
45mL is added in 0.3g sodium hydrogensulfite, 8g urea, 10g diammonium hydrogen phosphate and 5mL 0.4g/L methylene blue solution
It is dissolved completely in deionized water, obtains the uniform mother liquor of color；Ordinary filter paper is cut into the small cube of 1cm × 1cm, respectively according to
It is secondary be immersed in ethyl alcohol, 30min in deionized water, be dried for standby for 50 DEG C after taking-up；(3) by mother liquor in the addition of pretreated filter paper
In, after adding the ethyl alcohol of 4mL, ultrasonic vibration 20min takes out filter paper, and drying obtains the uniform target filter paper of color at 50 DEG C.
Prepared filter paper is fixed on the substrate not absorbed water with double-sided adhesive, prepares required target test paper.
Embodiment 3
0.3g sodium hydrogensulfite, 3g urea and 15g diammonium hydrogen phosphate and 5mL 0.4g/L methylene blue solution are added
It is dissolved completely in 45mL deionized water, obtains the uniform mother liquor of color；Ordinary filter paper is cut into the small cube of 1cm × 1cm, point
It is not immersed in ethyl alcohol successively, 30min in deionized water, is dried for standby for 50 DEG C after taking-up；It (3) will be in the addition of pretreated filter paper
In mother liquor, after adding the ethyl alcohol of 3mL, ultrasonic vibration 30min takes out filter paper, and drying obtains the uniform target of color at 50 DEG C
Filter paper.
Prepared filter paper is fixed on the substrate not absorbed water with double-sided adhesive, prepares required target test paper.
Embodiment 4
To determine effect of colour developing promotor during o-phthalaldehyde test paper color development, blank control test is done, i.e.,
The promotor that will plus develop the color under the same terms is compared with colour developing promotor is not added, and specific testing program is as follows:
A) 0.3g sodium hydrogensulfite, 5g urea and 10g diammonium hydrogen phosphate, 5mL 0.4g/L aqueous solution of methylene blue are added
It is dissolved completely in 45mL deionized water, obtains the uniform mother liquor of color；Ordinary filter paper is cut into the small cube of 1cm × 1cm, point
It is not immersed in ethyl alcohol successively, 30min in deionized water, is dried for standby for 50 DEG C after taking-up；It (3) will be in the addition of pretreated filter paper
In mother liquor, after adding the ethyl alcohol of 2mL, ultrasonic vibration 30min takes out filter paper, and drying obtains the uniform sample of color at 30 DEG C
Filter paper a.
B) 0.3g sodium hydrogensulfite, 5g urea and 5mL 0.4g/L aqueous solution of methylene blue are added in 45mL deionized water
Dissolution completely, obtains the uniform mother liquor of color；Ordinary filter paper is cut into the small cube of 1cm × 1cm, is successively immersed in second respectively
30min in alcohol, deionized water is dried for standby for 50 DEG C after taking-up；(3) it by mother liquor in the addition of pretreated filter paper, adds
After the ethyl alcohol of 2mL, ultrasonic vibration 30min takes out filter paper, and drying obtains the uniform sample filter paper b of color at 30 DEG C.
Prepared sample filter paper a and b are fixed on the substrate not absorbed water with double-sided adhesive respectively, are inserted into concentration 0.5%
O-phthalaldehyde solution in, compare the color change time, discovery addition colour developing promotor filter paper developing time about 90s, no
The filter paper developing time of addition colour developing promotor is about 5-6min.This comparative test demonstrates colour developing promotor in o-phthalaldehyde
The effect for accelerating chromogenic reaction is played in process color, and effect is extremely obvious.
Claims (5)
1. a kind of preparation method of O-phthalic aldehyde concentration quick detection test paper, which comprises the following steps: (1) will
Sodium hydrogensulfite, methylene blue, color developing agent and colour developing promotor are added dissolution in deionized water and completely, it is uniformly female to obtain color
Liquid；(2) ordinary filter paper is cut into the small cube of 1cm × 1cm, is successively immersed in ethyl alcohol, a period of time in deionized water respectively,
Drying obtains pretreatment filter paper after taking-up；(3) pretreatment filter paper is added in mother liquor, a certain amount of ethyl alcohol, ultrasound shake is added
It swings, obtains the uniform target filter paper of color after taking out drying；(4) filter paper prepared in (3) is fixed on double-sided adhesive and is not absorbed water
Substrate on, prepare required target test paper；The color developing agent is urea, and colour developing promotor is diammonium hydrogen phosphate, and color developing agent is dense
Degree is 60-160g/L, and colour developing promoter concentration is 200-300g/L.
2. a kind of preparation method of O-phthalic aldehyde concentration quick detection test paper according to claim 1, it is characterised in that:
The concentration of sodium bisulphite is 6g/L, and methylene blue concentration is 0.04g/L.
3. a kind of preparation method of O-phthalic aldehyde concentration quick detection test paper according to claim 1, it is characterised in that:
Filter paper soaking time in ethyl alcohol, deionized water is 20-40min in step (2)；Drying temperature is 30 DEG C -50 DEG C.
4. a kind of preparation method of O-phthalic aldehyde concentration quick detection test paper according to claim 1, it is characterised in that:
The ethyl alcohol volume being added in step (3) is the 4%-8% of mother liquor volume, and the ultrasonic vibration time is 30min, drying temperature 30
℃-50℃。
5. a kind of O-phthalic aldehyde concentration quick detection test paper that preparation method described in claim 1-4 any one obtains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710166963.2A CN106970075B (en) | 2017-03-20 | 2017-03-20 | A kind of o-phthalaldehyde Concentration Testing test paper and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710166963.2A CN106970075B (en) | 2017-03-20 | 2017-03-20 | A kind of o-phthalaldehyde Concentration Testing test paper and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106970075A CN106970075A (en) | 2017-07-21 |
| CN106970075B true CN106970075B (en) | 2019-05-10 |
Family
ID=59328962
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710166963.2A Active CN106970075B (en) | 2017-03-20 | 2017-03-20 | A kind of o-phthalaldehyde Concentration Testing test paper and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106970075B (en) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007240293A (en) * | 2006-03-08 | 2007-09-20 | Kayaku Co Ltd | Indicator for aldehyde type sterilizing agent |
| CN104215629A (en) * | 2013-06-05 | 2014-12-17 | 武汉海鹏医药科技有限公司 | Development and production of test paper for rapid testing of aldehyde disinfectant concentration |
| CN104677904A (en) * | 2015-02-04 | 2015-06-03 | 北京红辉力上科技有限公司 | Test paper for testing concentration of ortho-phthalaldehyde disinfectant |
| CN105548166B (en) * | 2015-12-30 | 2020-08-07 | 广州市医德生物科技有限责任公司 | Test paper for detecting concentration range of o-phthalaldehyde disinfectant |
-
2017
- 2017-03-20 CN CN201710166963.2A patent/CN106970075B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN106970075A (en) | 2017-07-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103674939A (en) | Mimic enzyme test paper for detecting hydrogen peroxide and application thereof | |
| CN103698314A (en) | Method for fast detection of residual sulfur dioxide in food | |
| CN106970075B (en) | A kind of o-phthalaldehyde Concentration Testing test paper and preparation method thereof | |
| DE60329679D1 (en) | TEST PROCEDURE WITH A SULPHONIC ACID AND A NITRO COMPOUND | |
| CN101839901A (en) | Digestion solution formula for fast determining chemical oxygen demand | |
| CN103822883A (en) | Method for detecting total chromium in water | |
| CN105755901A (en) | Rapid detection paper for disinfection residue content and preparation method thereof | |
| Masadome | Determination of cationic polyelectrolytes using a photometric titration with crystal violet as a color indicator | |
| CN116003421A (en) | Eight-membered cucurbituril supermolecule fluorescent complex and application thereof in detection of trinitrophenol | |
| CN104892469A (en) | Sensor molecule identifying cyanide ions by fluorescence and preparation and application of sensor molecule | |
| CN106883847A (en) | A kind of area's fluorescent quantum point probe of near-infrared two and preparation method and application | |
| CN113200935A (en) | Fluorescent probe for hypochlorous acid detection and preparation method thereof | |
| CN117405820A (en) | Rapid detection method for betaine synthesis reaction progress | |
| CN114870903B (en) | Mixed catalyst for dissolving trace colloidal iron in water vapor of power station | |
| KR890005509A (en) | Simultaneous Analysis of Calcium and Phosphorus | |
| KR20110128625A (en) | Nitrate concentration measuring method and the measuring kit using thereof | |
| RU2327156C1 (en) | Method of determining total reducing substances of lyes of sulfite pulping | |
| CN106596542A (en) | Synthesis method of ultraviolet fluorescence molecule probe, and nitrite ion detection of probe | |
| SU517844A1 (en) | Method for quantitative determination of epoxy groups in organic compounds | |
| RU2123691C1 (en) | Method of determining chemical oxygen minimum in circulating water- supply system utilized in phenol, ethylbenzene, and isopropylbezene production | |
| CN103436254A (en) | Functional multi-nitrogen heterocyclic ring fluorescent probe, preparation method and application thereof | |
| Rosenblatt et al. | Determination of Guaiacol in Presence of Large Amounts of Catechol | |
| SU615398A1 (en) | Method of quantitative determining of nitrosamines | |
| SU1442911A1 (en) | Method of analysing acetone | |
| SU1273796A1 (en) | Method of quantitative analysis of amine-containing compound |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |