CN106970075A - A kind of OPA Concentration Testing test paper and preparation method thereof - Google Patents
A kind of OPA Concentration Testing test paper and preparation method thereof Download PDFInfo
- Publication number
- CN106970075A CN106970075A CN201710166963.2A CN201710166963A CN106970075A CN 106970075 A CN106970075 A CN 106970075A CN 201710166963 A CN201710166963 A CN 201710166963A CN 106970075 A CN106970075 A CN 106970075A
- Authority
- CN
- China
- Prior art keywords
- filter paper
- test paper
- preparation
- paper
- opa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The present invention relates to a kind of OPA Concentration Testing test paper and preparation method thereof.The preparation method of the test paper is divided into mother liquor, filter paper pretreatment and the part of filter paper soaking and dyeing three.The colour developing principle of the filter paper is：After filter paper and OPA solution Rapid contact, a certain amount of solution of Adsorption of Filter Paper, if O-phthalic aldehyde concentration is not up to the threshold value specified（0.35%）When, the sodium bisulfite salts on filter paper consume all OPAs being adsorbed on filter paper and its true qualities are only presented（It is light blue）；If O-phthalic aldehyde concentration reaches the threshold value specified, the sodium bisulfite salts on filter paper consume part OPA on filter paper, remaining OPA and developer on test paper（Urea）In colour developing accelerator（Diammonium hydrogen phosphate）In the presence of rapidly reaction show yellow, then be superimposed the true qualities of test paper（It is light blue）It is in yellow green afterwards.The test paper detection method is easy to use, as a result accurately and reliably.
Description
Technical field
The present invention relates to field of chemical detection, and in particular to a kind of to carry out Concentration Testing to the thimerosal containing OPA
Test paper and preparation method thereof.
Background technology
OPA is as a kind of new medical disinfectant, and to ensure the effect of sterilization, its concentration need to be more than
0.35%.The method of detection O-phthalic aldehyde mainly has chemical titration, ultraviolet spectrophotometer method and efficient liquid at present
Phase analysis etc..But this several method all has cumbersome complexity, the shortcomings of time is longer is unsuitable for medical worker's sterilization
During at any time monitoring use.
OPA concentration test card has been enter into researcher's as a kind of method that can directly, rapidly determine concentration
The visual field.Such as Chinese patent CN 201310220855.0 (a kind of development and production of the quick test paper of aldehyde disinfectant concentration),
Chinese patent CN201511015840.6 (a kind of O-phthalaldehyde disinfectant concentration range Test paper), Chinese patent
CN201510058900.6 (a kind of OPA thimerosal Concentration Testing test paper) is studied concentration test card.But
It is that they have some limitations, a kind of such as entitled substantive examination of O-phthalaldehyde disinfectant concentration range Test paper is special
Profit is related to the modification of paper, operates complex；A kind of development of entitled quick test paper of aldehyde disinfectant concentration and
Detection time needed for the substantive examination patent of production is 5-8 minutes, and the time is more long.
The content of the invention
In order to solve problem described in background technology, the invention provides a kind of O-phthalic aldehyde concentration quick detection test paper
And the colour developing accelerator on paper.In the presence of colour developing accelerator, the test paper can according to the concentration of OPA rapidly,
Different degrees of color change is delicately shown, and then reflects the concentration of OPA.The test paper detection method uses simple
It is convenient, as a result accurately and reliably.
To reach the effect above, the present invention is adopted the following technical scheme that：
A kind of preparation method of O-phthalic aldehyde concentration quick detection test paper, comprises the following steps：(1) preparation of mother liquor：
Sodium hydrogensulfite, methylene blue, developer and colour developing accelerator are added in deionized water and dissolve complete, color is obtained uniform
Mother liquor；(2) pretreatment of filter paper：Ordinary filter paper is cut into 1cm × 1cm blockage, be immersed in successively respectively ethanol, go from
A period of time, dry for standby after taking-up in sub- water；(3) preparation of OPA Concentration Testing test paper：After being handled in (2)
In filter paper addition (1) in mother liquor, add after a certain amount of ethanol, ultrasonic vibration for a period of time, takes out filter paper, and drying obtains face
The uniform target filter paper of color；(4) filter paper prepared in (3) is fixed on the base material not absorbed water with double faced adhesive tape, needed for preparing
Target test paper.
Preferably, described concentration of sodium bisulphite is 6g/L, and methylene blue concentration is 0.04g/L, and chromogenic agent is
60-160g/L, colour developing promoter concentration is 200-300g/L；
Step (2) filter paper soak time in ethanol, deionized water is 20-40min；Drying temperature is 30-50 DEG C；
The ethanol volumetric concentration that step (3) is added is the 4%-8% of mother liquor volume；The ultrasonic vibration time is 30min, drying
Temperature is 30 DEG C -50 DEG C.
A kind of O-phthalic aldehyde concentration quick detection test paper, is prepared by the above method.
The principle of the present invention is to utilize the change of color change display density.
It is specific as follows：When O-phthalic aldehyde concentration is not up to threshold value (0.35%) specified, the bisulfite on filter paper
Sodium salt consume it is all be adsorbed in OPA on filter paper (OPA preferentially with sodium hydrogensulfite reactant salt, then with it is aobvious
Toner reacts), its reactional equation is as follows：
C6H4(CHO)2+2NaHSO3→C6H4(CHOHSO3Na)2
Product shows colourless.Because the true qualities of the test paper are light blue, so filter paper is still light blue color during detection；Work as neighbour
When phthalaldehyde concentration reaches threshold value (0.35%) specified, the sodium bisulfite salts on filter paper consume partial adsorbates in filter paper
Upper OPA, i.e. OPA have residue.Now, on test paper developer (urea) colour developing accelerator (phosphoric acid hydrogen two
Ammonium) in the presence of rapidly reacted with remaining OPA on filter paper and show yellow, then be superimposed test paper true qualities it is (light blue
Color) it is in yellow green afterwards.Wherein, the colour developing principle of developer is the imines chemical combination generated based on urea and O-phthalic aldehyde reaction
Thing is in yellow, reaction equation such as following formula：
NH2CONH2+C6H4(CHO)2→C6H4(CNH)2CO+2H2O
On the other hand, to determine effect of the colour developing accelerator during OPA test paper color development, blank pair has been done
Concentration 0.5% is inserted according to the Test paper that will add colour developing accelerator under experiment, i.e. the same terms and be not added with colour developing accelerator making
OPA solution in, contrast color change when the time, find addition colour developing accelerator filter paper developing time about 90s,
Filter paper developing time without colour developing accelerator is about 5-6min.This contrast test demonstrates colour developing accelerator in O-phthalic
The effect for accelerating chromogenic reaction is served in aldehyde process color, and effect is extremely obvious.
The present invention has advantages below and beneficial effect：
(1) color change of the invention is shown substantially (from the light blue change to yellow green)；
(2) Coloring Time of the invention is short (90s can develop the color)；
(3) present invention is simple, convenient, sensitive.
Brief description of the drawings
Fig. 1 OPAs Test paper detects various concentrations OPA solution colour developing figure
From left to right：O-phthalic aldehyde concentration is followed successively by 0.1%, 0.2%, 0.3%, 0.4%, 0.5%.
Embodiment
By combination accompanying drawing described further below it will be further appreciated that the features and advantages of the invention.The implementation provided
Example is only the explanation to the inventive method, without limiting remaining content that the present invention is disclosed in any way.
Embodiment 1
0.3g sodium hydrogensulfites, 5g urea and 10g diammonium hydrogen phosphates, 5mL 0.4g/L aqueous solution of methylene blue are added
Dissolve complete in 45mL deionized waters, obtain the uniform mother liquor of color；Ordinary filter paper is cut into 1cm × 1cm blockage, point
It is not immersed in 30min in ethanol, deionized water successively, 50 DEG C of dry for standby after taking-up；(3) during pretreated filter paper is added
In mother liquor, after the ethanol for adding 2mL, ultrasonic vibration 30min takes out drying at filter paper, 30 DEG C and obtains the uniform target of color
Filter paper.
Prepared filter paper is fixed on the base material not absorbed water with double faced adhesive tape, the target test paper needed for preparing.
The filter paper fixed is inserted into 3s in surveyed solution, it is light to dry after taking out, read face after starting timing, 90s
Color.
Fig. 1 is respectively O-phthalic aldehyde concentration when being 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, and test paper shows after 90s
Pornographic condition.As can be seen that concentration is 0.1%, filter paper is in still primary colors (light blue) when 0.2%, starts discoloration when 0.3%, but
It is that color is shallower also uneven, yellow green is obvious when concentration reaches 0.4% and color is uniform.It can draw, the filter paper is to adjacent benzene
The detection speed of dicarbaldehyde is fast, effect is good.
Embodiment 2
0.3g sodium hydrogensulfites, 8g urea, 10g diammonium hydrogen phosphates and 5mL 0.4g/L methylene blue solutions are added into 45mL
Dissolve complete in deionized water, obtain the uniform mother liquor of color；Ordinary filter paper is cut into 1cm × 1cm blockage, respectively according to
It is secondary to be immersed in 30min in ethanol, deionized water, 50 DEG C of dry for standby after taking-up；(3) mother liquor during pretreated filter paper is added
In, after the ethanol for adding 4mL, ultrasonic vibration 20min takes out drying at filter paper, 50 DEG C and obtains the uniform target filter paper of color.
Prepared filter paper is fixed on the base material not absorbed water with double faced adhesive tape, the target test paper needed for preparing.
Embodiment 3
0.3g sodium hydrogensulfites, 3g urea and 15g diammonium hydrogen phosphates and 5mL 0.4g/L methylene blue solutions are added
Dissolve complete in 45mL deionized waters, obtain the uniform mother liquor of color；Ordinary filter paper is cut into 1cm × 1cm blockage, point
It is not immersed in 30min in ethanol, deionized water successively, 50 DEG C of dry for standby after taking-up；(3) during pretreated filter paper is added
In mother liquor, after the ethanol for adding 3mL, ultrasonic vibration 30min takes out drying at filter paper, 50 DEG C and obtains the uniform target of color
Filter paper.
Prepared filter paper is fixed on the base material not absorbed water with double faced adhesive tape, the target test paper needed for preparing.
Embodiment 4
To determine effect of the colour developing accelerator during OPA test paper color development, blank control test is done, i.e.,
Accelerator of plus being developed the color under the same terms compares with being not added with colour developing accelerator, and specific testing program is as follows：
A) 0.3g sodium hydrogensulfites, 5g urea and 10g diammonium hydrogen phosphates, 5mL 0.4g/L aqueous solution of methylene blue are added
Dissolve complete in 45mL deionized waters, obtain the uniform mother liquor of color；Ordinary filter paper is cut into 1cm × 1cm blockage, point
It is not immersed in 30min in ethanol, deionized water successively, 50 DEG C of dry for standby after taking-up；(3) during pretreated filter paper is added
In mother liquor, after the ethanol for adding 2mL, ultrasonic vibration 30min takes out drying at filter paper, 30 DEG C and obtains the uniform sample of color
Filter paper a.
B) 0.3g sodium hydrogensulfites, 5g urea and 5mL 0.4g/L aqueous solution of methylene blue are added in 45mL deionized waters
Dissolving is complete, obtains the uniform mother liquor of color；Ordinary filter paper is cut into 1cm × 1cm blockage, second is immersed in successively respectively
30min in alcohol, deionized water, 50 DEG C of dry for standby after taking-up；(3) during pretreated filter paper is added in mother liquor, add
After 2mL ethanol, ultrasonic vibration 30min takes out drying at filter paper, 30 DEG C and obtains the uniform sample filter paper b of color.
Prepared sample filter paper a and b are fixed on the base material not absorbed water with double faced adhesive tape respectively, concentration 0.5% is inserted
OPA solution in, contrast the color change time, find addition colour developing accelerator filter paper developing time about 90s, no
The filter paper developing time of addition colour developing accelerator is about 5-6min.This contrast test demonstrates colour developing accelerator in OPA
The effect for accelerating chromogenic reaction is served in process color, and effect is extremely obvious.
Claims (6)
1. a kind of preparation method of O-phthalic aldehyde concentration quick detection test paper, it is characterised in that comprise the following steps：（1）Will
Sodium hydrogensulfite, methylene blue, developer and colour developing accelerator, which are added in deionized water, dissolves complete, obtains color uniformly female
Liquid；（2）Ordinary filter paper is cut into the cm of 1 cm × 1 blockage, when being immersed in one section in ethanol, deionized water successively respectively
Between, drying obtains pre-processing filter paper after taking-up；（3）Pretreatment filter paper is added in mother liquor, a certain amount of ethanol, ultrasound is added
Concussion, the uniform target filter paper of color is obtained after taking out drying；（4）Will（3）The filter paper of middle preparation is fixed on double faced adhesive tape not to be inhaled
On the base material of water, the target test paper needed for preparing.
2. a kind of preparation method of O-phthalic aldehyde concentration quick detection test paper according to claim 1, its feature exists
In：Developer is urea, and colour developing accelerator is diammonium hydrogen phosphate, and chromogenic agent is 60-160 g/L, and develop the color promoter concentration
For 200-300 g/L.
3. a kind of preparation method of O-phthalic aldehyde concentration quick detection test paper according to claim 1, it is characterised in that：
Described concentration of sodium bisulphite is 6 g/L, and methylene blue concentration is 0.04 g/L.
4. a kind of preparation method of O-phthalic aldehyde concentration quick detection test paper according to claim 1, it is characterised in that：
Step（2）Middle filter paper soak time in ethanol, deionized water is 20-40 min；Drying temperature is 30 DEG C -50 DEG C.
5. a kind of preparation method of O-phthalic aldehyde concentration quick detection test paper according to claim 1, it is characterised in that：
Step（3）The ethanol volume of middle addition is the 4%-8% of mother liquor volume, and the ultrasonic vibration time is 30 min, and drying temperature is 30
℃-50 ℃。
6. a kind of O-phthalic aldehyde concentration quick detection test paper that the preparation method described in claim 1-5 any one is obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710166963.2A CN106970075B (en) | 2017-03-20 | 2017-03-20 | A kind of o-phthalaldehyde Concentration Testing test paper and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710166963.2A CN106970075B (en) | 2017-03-20 | 2017-03-20 | A kind of o-phthalaldehyde Concentration Testing test paper and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106970075A true CN106970075A (en) | 2017-07-21 |
| CN106970075B CN106970075B (en) | 2019-05-10 |
Family
ID=59328962
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710166963.2A Active CN106970075B (en) | 2017-03-20 | 2017-03-20 | A kind of o-phthalaldehyde Concentration Testing test paper and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106970075B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007240293A (en) * | 2006-03-08 | 2007-09-20 | Kayaku Co Ltd | Indicator for aldehyde type sterilizing agent |
| CN104215629A (en) * | 2013-06-05 | 2014-12-17 | 武汉海鹏医药科技有限公司 | Development and production of test paper for rapid testing of aldehyde disinfectant concentration |
| CN104677904A (en) * | 2015-02-04 | 2015-06-03 | 北京红辉力上科技有限公司 | Test paper for testing concentration of ortho-phthalaldehyde disinfectant |
| CN105548166A (en) * | 2015-12-30 | 2016-05-04 | 广州市医德生物科技有限责任公司 | OPA (o-phthalaldehyde) disinfector concentration range detection test paper |
-
2017
- 2017-03-20 CN CN201710166963.2A patent/CN106970075B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007240293A (en) * | 2006-03-08 | 2007-09-20 | Kayaku Co Ltd | Indicator for aldehyde type sterilizing agent |
| CN104215629A (en) * | 2013-06-05 | 2014-12-17 | 武汉海鹏医药科技有限公司 | Development and production of test paper for rapid testing of aldehyde disinfectant concentration |
| CN104677904A (en) * | 2015-02-04 | 2015-06-03 | 北京红辉力上科技有限公司 | Test paper for testing concentration of ortho-phthalaldehyde disinfectant |
| CN105548166A (en) * | 2015-12-30 | 2016-05-04 | 广州市医德生物科技有限责任公司 | OPA (o-phthalaldehyde) disinfector concentration range detection test paper |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106970075B (en) | 2019-05-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107478621B (en) | Metabolizable generation H in quantitative detection serum2O2Biomolecule method | |
| Halfpenny et al. | 168. Pernitrous acid. The reaction between hydrogen peroxide and nitrous acid, and the properties of an intermediate product | |
| CN108828128B (en) | A method of total Organic fluoride in measurement water sample and aqueous solution | |
| CN113801105B (en) | Mitochondrion targeted peroxynitrite/bisulfite dual-response fluorescent probe | |
| CN104792714A (en) | Measurement method for permanganate index and application | |
| CN106970075A (en) | A kind of OPA Concentration Testing test paper and preparation method thereof | |
| Christy et al. | Oxidation of thiocyanate by hydrogen peroxide—a reaction kinetic study by capillary electrophoresis | |
| CN101839901A (en) | Digestion solution formula for fast determining chemical oxygen demand | |
| CN109342419A (en) | A kind of methanol in wine quick testing reagent and its application method | |
| CN103436252A (en) | Anion fluorescent probe for forming aggregate through anion inducing | |
| Lowen et al. | Determination of 3-(p-Chlorophenyl)-1, 1-dimethylurea | |
| Masadome | Determination of cationic polyelectrolytes using a photometric titration with crystal violet as a color indicator | |
| Elofson et al. | Amperometric Titration with Diazotized Aromatic Amines | |
| CN116003421A (en) | Eight-membered cucurbituril supermolecule fluorescent complex and application thereof in detection of trinitrophenol | |
| Nakamura | Resorcinol as fluorimetric reagent for the determination of nitrate | |
| CN109232397A (en) | It is a kind of to pass through the alkaline accurate pH test paper and its preparation method and application of washing recycling | |
| CN104892469A (en) | Sensor molecule identifying cyanide ions by fluorescence and preparation and application of sensor molecule | |
| CN106883847A (en) | A kind of area's fluorescent quantum point probe of near-infrared two and preparation method and application | |
| CN103134787A (en) | Application method of ratio-type cyanide ion fluorescent probe molecule | |
| Kothainayaki et al. | Study of solvent dependence and kinetics of proton transfer reactions of 3-hydroxybiphenyl in the excited singlet state | |
| CN103528976A (en) | Method for determination of content of phosphorus in water | |
| Miyata | Spectrophotometric Determination of the Acid Dissociation Constants of Plasmocorinth B | |
| KR20110128625A (en) | Nitrate concentration measuring method and the measuring kit using thereof | |
| RU2366931C1 (en) | Method for determination of reducing substances content in sugar-containing media | |
| CN116239530A (en) | Fluorescent probe for detecting hypochlorite and phosgene, and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |