CN106967753A - Prince's-feather fermenting plant promoting bacteria and its application in preventing and treating pest and disease damage - Google Patents
Prince's-feather fermenting plant promoting bacteria and its application in preventing and treating pest and disease damage Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/30—Polygonaceae [Buckwheat family], e.g. red-knees or rhubarb
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract
The invention provides a kind of method that prince's-feather that ferments prepares insecticide, its process characteristic is using prince's-feather as primary raw material, it is aided with house refuse, starchy material and water, using commercially available bacillus pumilus, Su Yun bacillus and bacillus amyloliquefaciens H47 as fermentation strain, according to prince's-feather：House refuse：Starchy material:Pure water=2~5:1~2:0.01:3~7 ratio and the inoculation amount of system 2~5%, fermentation temperature are 25~35 DEG C, and rotating speed is 100~200rpm, keeps pH=5~8,6~9d of fermentation time.Complete fermentation of the present invention, makes a variety of enzymes therein, vitamin, amino acid and trace element fully discharge, product is improved the drug effect and its function of insecticide.And do not influence the physiological activator of botanical pesticide again.
Description
Technical field
A kind of method that microbial fermentation prepares insecticide, is related to and is conciliate with commercially available bacillus pumilus, Su Yun bacillus
Bacillus amyloliquefacienses H47 freeze-dried powders, the method that insecticide is prepared by fermenting raw materials of prince's-feather belongs to bioengineering and fermentation skill
Art field.
Background technology
Prince's-feather is polygonaceae annual herb plant.Its stem is upright, sturdy, high 1-2m, top multi-branched, close to be carried out
Long pubescence.Leaf width is avette, wide oval or ovum shape lanceolar, and long 10-20cm, wide 5-12cm, top is tapering, basal circular or near
Close growth pubescence on heart, micro- downward, edge full edge, close raw echinid, the close raw pubescence in two sides, vein；The long 2-10cm of petiole, tool
The long pubescence carried out；Ochrea tubular, film quality, long 1-2cm, villosity has long echinid, generally has herbaceous stem, green along top
Wing.Raceme is in spike, basidixed or axillary, and long 3-7cm spends close, micro- sagging, and usual several recompositions are coniform；Bud
The wide funnel-form of piece, long 3-5mm, herbaceous stem, green, by pubescence, edge has long echinid, the interior tool 3-5 flowers per bud；Bennet compares bract
It is long；The drastic crack of perianth 5, pale red or white；Tapel ellipse, long 3-4mm；Stamen 7, it is longer than perianth；Floral disc is obvious；Style 2,
Middle and lower part symphysis, column cap head longer than perianth.Achene subcircular, concave-concave, the long 3-3.5mm of diameter, dark brown is glossy, is wrapped in
In persitent perianth.The month at florescence 6-9, the fruiting period 8-10 months.Prince's-feather is widely distributed in all over China, wild or cultivation.Raw limes marginis wetland, village
By wing, 30-2700 meters of height above sea level.Prince's-feather contains quercitrin, 5,6,7,8- hexa methoxy -4', 5'- methylene-dioxy flavones；5-
Hydroxyl -3,3', 6,7,8- pentamethoxyls -4 ', 5- methylene-dioxy flavones；3'- hydroxyl -3,4 ', 5,5 ', 6,7,8- seven methoxyl groups
Flavones；3,3 ', 5,8- tetramethoxies -4 ', 5', 6,7- double (methylene-dioxy) flavones；3'- hydroxyls -3,4', 5, the first of 5 ', 8- five
Epoxide -6,7- methylene-dioxy flavones；3', 5- dihydroxy -3,4', 5', 8- tetramethoxy -6,7- methylene-dioxy flavones；3,
3', 4 ', 5,5 ', 8- hexa methoxy -6,7- methylene-dioxy flavones；Digicitrine；Month tangerine element；Leaf contains orientin, orientoside
A、B；The secondary metabolites such as Vitexin.Prince's-feather extract is to armyworm, Culex pipiens pallens, diamondback moth, black peach aphid, Tetranychus cinnabarinus；It is small
Wheat powdery mildew, cucumber downy mildew, cucumber anthracnose have certain killing and preventive and therapeutic effect.But botanical pesticide and antiseptic
Multi-purpose organic solvent or decoct is extracted, 1) it is unfavorable for saving and also has larger environmental risk；2) stability of active material is influenceed
With loss active material；3) increase cost make it that development and application difficulty is larger, and action effect is slow, therefore to plant insecticide
Research and development and production bring huge financial difficulties.Using suitable plant growth-promoting bacteria fermentation prince's-feather, life is not only contributed to
The extraction of active material is managed, is also less prone to cause the inactivation of active material, Promoting bacteria also has preferable disinsection prophylaxis and growth-promoting in itself
Effect.Be conducive to improving plant growth and strengthen insecticidal effect.Be conducive to the effective utilization of environmental protection and prince's-feather in itself.
The content of the invention
The present invention is carried out fermentation by microorganism and is produced various secondary metabolites and plant using the affluent resources of prince's-feather
Promoting bacteria, which produces the multiclass such as enzyme, alcohols and aromatic substance, has adopted metabolite so as to improve the drug effect and its function of insecticide,
It is short that the present invention possesses fermentation time, harmful microorganism grow it is few even without, it is to avoid produced due to crossing lengthy fermentation
Virus, and the advantages of the physiological activators such as lipopeptid can be produced, so as to improve effect of disinsection prophylaxis and the rush of plant growth
Raw effect.
Inventive formulation and usage：
(1) selected material：It is fermentation main material to select the measured prince's-feather 1kg/L of fresh, matter.
(2) processing of material：First rinsed with running water, a century of Wuxi a century Shang Shui drinks Co., Ltd offer is provided
Still water clear water is washed twice, is finally invaded and steeped 120 seconds with 0.9% NaCl solution again, drained and standby.
(3) Spawn incubation：The century-old still water provided using Wuxi a century Shang Shui drinks Co., Ltd prepares LB culture mediums, will
Original preserves bacterial strain activation, cultivates the bacterium solution obtained for about 48 hours standby；
(4) inoculation fermentation：According to inoculative proportion 4%, commercially available bacillus pumilus, Su Yun bacillus are conciliate into starch gemma
Bacillus H47 accesses fermenation raw liquid according to a certain percentage, and each bacteria concentration is 106CFU/ml, ferment 3~9d, rotating speed be 100~
200r/min, pH value 5.0~8.0,25 DEG C~35 DEG C of temperature.
(5) post-processing makes：Coarse filtration, tentatively screens out bulky grain residue, it is to avoid prince's-feather, which is put to easily cause long, to become sour,
Filter condition：Sieves number is 20~32 mesh.Thin filtering：Small particle residue is further filtered, liquid is presented limpider
State, filter condition：Sieves number is 80~100 mesh, has both been able to the insecticide of prince's-feather fermentation raw material.
The preparation method of step (1) raw material comprises the following steps：
(11) instrument, utensil used in are intended to sterilizing, without greasy.
(12) prince's-feather is cleaned with pure water, then drained with the saline sook of pure water or 0.9%.
(13) prince's-feather is placed in sterilized container again, it is rear to add house refuse and starchy material and pure water,
Volume ratio prince's-feather wherein in fermenation raw liquid：House refuse：Starchy material:Pure water=4:1~2:0.01:4~5, produce
Prince's-feather fermenation raw liquid.
The beneficial effect of invention：
This not only contributes to the extraction of physiological activator using suitable plant growth-promoting bacteria fermentation prince's-feather, keeps living
Property material stability, Promoting bacteria can inhibit other miscellaneous bacterias to grow so as to there is preferable disinsection prophylaxis by dominant strain in itself
And growth-promoting functions.Be conducive to improving plant growth and strengthen insecticidal effect.Be conducive to environmental protection and prince's-feather plant in itself
Effectively utilize.Improve the economic value of insecticide.
Embodiment
Below in conjunction with the embodiment of the present invention, technical scheme is carried out to describe clear, perfectly, it is clear that institute
The embodiment of description is only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention,
The other embodiment that those of ordinary skill in the art are obtained on the premise of creative work is not made, belongs to the present invention
The scope of protection.
Embodiment 1
A kind of method that insecticide is prepared by fermenting raw materials of prince's-feather, comprises the following steps：
(1) the selection measured prince's-feather 5g of fresh, matter is as fermentation primary raw material.
(2) pure water clear water is washed twice, is finally soaked, then cold sterilization drains, filled with 0.9% NaCl solution again
Enter in the cleaned container of pure water, it is fermentation to add and account for the house refuse and 0.5% starchy material of total liquid volume 5%
Stoste is standby.
(3) LB culture mediums are prepared, original is preserved into bacterial strain activation, the zymophyte that 100r/min shaken cultivations are obtained for about 48 hours
Liquid is standby；
(4) commercially available bacillus pumilus, Su Yun bacillus and bacillus amyloliquefaciens H47 are pressed according to inoculative proportion 4%
Deng than taking access fermenation raw liquid, 120r/min shaken cultivations fermentation 12d, pH value 6.0,25 DEG C of temperature respectively.
(5) coarse filtration is first carried out, sieves number is 20~32 mesh.Then thin filtering, sieves number is 80~100
Mesh, produces the insecticide that prince's-feather is fermentation raw material.
Embodiment 2
A kind of method that insecticide is prepared by fermenting raw materials of prince's-feather, comprises the following steps：
(1) the selection measured prince's-feather 5g of fresh, matter is as fermentation primary raw material.
(2) pure water clear water is washed twice, is finally soaked, then cold sterilization drains, filled with 0.9% NaCl solution again
Enter in the cleaned container of pure water, it is fermentation to add and account for the house refuse and 1% starchy material of total liquid volume 10%
Stoste is standby.
(3) LB culture mediums are prepared, original is preserved into bacterial strain activation, the zymophyte that 100r/min shaken cultivations are obtained for about 48 hours
Liquid is standby；
(4) commercially available bacillus pumilus, Su Yun bacillus and bacillus amyloliquefaciens H47 are pressed according to inoculative proportion 5%
Deng than taking access fermenation raw liquid, 120r/min shaken cultivations fermentation 9d, pH value 6.0,28 DEG C of temperature respectively.
(5) coarse filtration is first carried out, sieves number is 20~32 mesh.Then thin filtering, sieves number is 80~100
Mesh, produces the insecticide that prince's-feather is fermentation raw material.
Embodiment 3
A kind of method that insecticide is prepared by fermenting raw materials of prince's-feather, comprises the following steps：
(1) the selection measured prince's-feather 5g of fresh, matter is as fermentation primary raw material.
(2) pure water clear water is washed twice, is finally soaked, then cold sterilization drains, filled with 0.9% NaCl solution again
Enter in the cleaned container of pure water, it is hair to add and account for the house refuse and 1.5% starchy material of total liquid volume 15%
Ferment stoste is standby.
(3) LB culture mediums are prepared, original is preserved into bacterial strain activation, the zymophyte that 100r/min shaken cultivations are obtained for about 48 hours
Liquid is standby；
(4) commercially available bacillus pumilus, Su Yun bacillus and bacillus amyloliquefaciens H47 are pressed according to inoculative proportion 5%
Deng than taking access fermenation raw liquid, 120r/min shaken cultivations fermentation 6d, pH value 6.0,30 DEG C of temperature respectively.
(5) coarse filtration is first carried out, sieves number is 20~32 mesh.Then thin filtering, sieves number is 80~100
Mesh, produces the insecticide that prince's-feather is fermentation raw material.
Embodiment 4
A kind of method that insecticide is prepared by fermenting raw materials of prince's-feather, comprises the following steps：
(1) the selection measured prince's-feather 5g of fresh, matter is as fermentation primary raw material.
(2) pure water clear water is washed twice, is finally soaked, then cold sterilization drains, filled with 0.9% NaCl solution again
Enter in the cleaned container of pure water, it is fermentation to add and account for the house refuse and 2% starchy material of total liquid volume 20%
Stoste is standby.
(3) LB culture mediums are prepared, original is preserved into bacterial strain activation, the zymophyte that 100r/min shaken cultivations are obtained for about 48 hours
Liquid is standby；
(4) commercially available bacillus pumilus, Su Yun bacillus and bacillus amyloliquefaciens H47 are pressed according to inoculative proportion 5%
Deng than taking access fermenation raw liquid, 120r/min shaken cultivations fermentation 3d, pH value 6.0,35 DEG C of temperature respectively.
(5) coarse filtration is first carried out, sieves number is 20~32 mesh.Then thin filtering, sieves number is 80~100
Mesh, produces the insecticide that prince's-feather is fermentation raw material.
Prince's-feather in the present invention contains abundant cometabolism material and prince's-feather ether extract and alcohol extract etc. to planting
Thing has growth-promoting functions and drug effect substantially, but is not limited only to this, according to actual conditions, can determine prince's-feather fresh weight or
Be dry weight addition or selection starchy material and domestic garbage mixing together, as long as can reach insecticide proportioning conjunction
Reason, the drug effect of insecticide can just play obvious effect.
The selection of other fermenation raw liquid and proportioning, connect bacterium amount and be also not necessarily limited to the combination that embodiment is lifted, can be according to actual feelings
Condition selects different combinations.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, all thinkings in the present invention
Within principle, any modification, equivalent substitution and improvements done etc. should be included in the scope of the protection.
Claims (9)
1. a kind of technology that insecticide is prepared by fermenting raw materials of prince's-feather, is characterized in using the measured prince's-feather of matter as main hair
Ferment raw material, adds starch and house refuse, using commercially available bacillus pumilus, Su Yun bacillus and bacillus amyloliquefaciens H47, raw
Production prepares insecticide, and its technique is：
1) surface contaminants are cleaned with running water from the fresh prince's-feather without lesion, reuses the century-old still limited public affairs of water drink in Wuxi
The century-old still water clear water that department provides is washed twice, is finally invaded and steeped 120 seconds with 0.9% NaCl solution again, drained, be put into tank, press
Volume ratio is prince's-feather：House refuse：Starchy material:Pure water=4:1~2:0.01:4~5 are configured to fermenation raw liquid.
2) LB culture mediums are prepared, original is preserved into bacterial strain activation, the bacterium solution obtained for about 48 hours is cultivated standby；Again by commercially available short and small bud
Spore bacillus, Su Yun bacillus and bacillus amyloliquefaciens H47 access fermenation raw liquid according to a certain percentage
3) in fermentation system, the strain of system 4% is added, fermentation temperature is 25~35 DEG C, and rotating speed is 100~200rpm, is kept
PH=5~8,6~9d of fermentation time.
4) zymotic fluid after the completion of) fermenting is first through coarse filtration, and sieves number is 20~32 mesh；Thin filtering, sieves number again
For 80~100 mesh, you can prepare the insecticide using prince's-feather as raw material.
2. the method that a kind of prince's-feather that ferments according to claim 1 prepares insecticide, it is characterized in that：Compare according to inoculation
Example 4%, fermenation raw liquid, each bacteria concentration are accessed by commercially available bacillus pumilus, Su Yun bacillus and bacillus amyloliquefaciens H47 grade ratios
For 106CFU/ml, rotating speed is 100~200rpm, and ferment 3~9d, pH value 5.0~8.0,25 DEG C~35 DEG C of temperature.
3. the method that a kind of prince's-feather that ferments according to claim 1 prepares insecticide, it is characterized in that：The LB cultures of preparation
Base is：Tryptone 10g, yeast extract 5g, sodium chloride 10g, constant volume to 1L adjust pH=7.0.
4. the method that a kind of prince's-feather that ferments according to claim 1 prepares insecticide, it is characterized in that：Fermenation raw liquid volume
Than for prince's-feather：House refuse：Starchy material:Pure water=4:1~2:0.01:4~5.
5. the method that a kind of prince's-feather that ferments according to claim 1 prepares insecticide, it is characterized in that：Fermentation strain is used
It is CCTCC M's 2013283 to be stored in the deposit number of China typical culture collection center on June 24th, 2013
Bacillus amyloliquefaciens H47 Bacillus amyloliquefaciens strain is fermentation strain.
6. the method that a kind of prince's-feather that ferments according to claim 1 prepares insecticide, it is characterized in that：The addition of strain
For the 4% of system.
7. the method that a kind of prince's-feather that ferments according to claim 1 prepares insecticide, it is characterized in that：The processing side of raw material
Method is to clean surface contaminants from the fresh prince's-feather running water without lesion, bubble is invaded with 0.9% NaCl solution 120 seconds, drip
It is dry standby.
8. the method that a kind of prince's-feather that ferments according to claim 1 prepares insecticide, it is characterized in that：Raw material and strain,
House refuse and starchy material are well mixed, and fermentation temperature is 25~35 DEG C, and 100~200rpm of rotating speed keeps pH=5~8,
6~9d of fermentation time.
9. the method that a kind of prince's-feather that ferments according to claim 1 prepares insecticide, it is characterized in that：After the completion of fermentation
Zymotic fluid is first through coarse filtration, and sieves number is 20~32 mesh；Thin filtering again, sieves number is 80~100 mesh.
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| CN201710243502.0A CN106967753A (en) | 2017-04-14 | 2017-04-14 | Prince's-feather fermenting plant promoting bacteria and its application in preventing and treating pest and disease damage |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710243502.0A CN106967753A (en) | 2017-04-14 | 2017-04-14 | Prince's-feather fermenting plant promoting bacteria and its application in preventing and treating pest and disease damage |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113817634A (en) * | 2021-09-07 | 2021-12-21 | 江西省科学院微生物研究所 | Bacillus amyloliquefaciens Bam-6 and preparation and application thereof |
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|---|---|---|---|---|
| CN1265834A (en) * | 1999-03-07 | 2000-09-13 | 宛吉斌 | Process for preparing natural plant biological biocide and biological fertilizer |
| CN101697740A (en) * | 2009-04-04 | 2010-04-28 | 冯子龙 | Novel traditional Chinese medicine pesticide and production technology thereof |
| CN103451128A (en) * | 2013-07-12 | 2013-12-18 | 江南大学 | Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease |
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2017
- 2017-04-14 CN CN201710243502.0A patent/CN106967753A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1265834A (en) * | 1999-03-07 | 2000-09-13 | 宛吉斌 | Process for preparing natural plant biological biocide and biological fertilizer |
| CN101697740A (en) * | 2009-04-04 | 2010-04-28 | 冯子龙 | Novel traditional Chinese medicine pesticide and production technology thereof |
| CN103451128A (en) * | 2013-07-12 | 2013-12-18 | 江南大学 | Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease |
Non-Patent Citations (1)
| Title |
|---|
| 林妍妍: "东方蓼提取物杀虫杀菌活性初探", 《农药》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113817634A (en) * | 2021-09-07 | 2021-12-21 | 江西省科学院微生物研究所 | Bacillus amyloliquefaciens Bam-6 and preparation and application thereof |
| CN113817634B (en) * | 2021-09-07 | 2023-01-17 | 江西省科学院微生物研究所 | Bacillus amyloliquefaciens Bam-6 and preparation and application thereof |
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Application publication date: 20170721 |