CN106962351A - Bactericidal composition containing Chinese yam polysaccharide and fluazinam - Google Patents
Bactericidal composition containing Chinese yam polysaccharide and fluazinam Download PDFInfo
- Publication number
- CN106962351A CN106962351A CN201710298614.6A CN201710298614A CN106962351A CN 106962351 A CN106962351 A CN 106962351A CN 201710298614 A CN201710298614 A CN 201710298614A CN 106962351 A CN106962351 A CN 106962351A
- Authority
- CN
- China
- Prior art keywords
- fluazinam
- chinese yam
- yam polysaccharide
- polysaccharide
- bactericidal composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of bactericidal composition containing Chinese yam polysaccharide and fluazinam, active component is made up of Chinese yam polysaccharide and fluazinam, and the weight ratio of described Chinese yam polysaccharide and fluazinam in the composition is 0.1 5:1.The Chinese yam polysaccharide and fluazinam of the present invention is 0.1 5 according to weight ratio:1 is compounded, with obvious synergistic function, Chinese yam polysaccharide as fluazinam synergist, inhibitory activity of the fluazinam to germ can be significantly improved, the two mixture can also reduce the use of chemical agent fluazinam, so as to reduce cost, reduce the residual of chemical agent.
Description
Technical field
The present invention relates to disinfectant use in agriculture field, more particularly to a kind of bactericidal composition containing Chinese yam polysaccharide and fluazinam.
Background technology
Chinese yam polysaccharide is the main active in Chinese yam.Chinese yam polysaccharide is main by mannose, xylose, arabinose, Portugal
Grape sugar and galactolipin composition.Research shows that Chinese yam is extracted through hot water, deproteinized, ethanol precipitation, SephadexG-100 are purified
Chinese yam polysaccharide, molecular weight is 8.1 × 10-4.Current people are concentrated mainly on following aspect for the research of Chinese yam polysaccharide：Blood drops
The functions such as sugar, tonifying middle-Jiao and Qi, stimulation or regulation immune system, and the research for its bacteria resistance function is also fewer.
Chinese patent CN201410143035.0 discloses a kind of isolation and purification method of Chinese yam polysaccharide, including pre-treatment,
Hot water water-bath extraction, Sevage methods removing protein, rough segmentation polysaccharide and SephadexG-100 are carried out using high-speed countercurrent chromatography chromatographed
Post such as is further purified at the step, meanwhile, the patent also measured were the anti-oxidant and bacteriostatic activity of Chinese yam polysaccharide, and its result shows,
Chinese yam polysaccharide without bacteriostasis, has suppression to bacillus subtilis, staphylococcus aureus, Nissl bacillus to Escherichia coli
Bacterium acts on, but not substantially, determines minimal inhibitory concentration with doubling dilution, learn Chinese yam polysaccharide to the antibacterial dense of Escherichia coli
Spend for 2.5mg/mL.
It is open that Chinese yam polysaccharide yet there are no research for the inhibitory action of fungi.
Fluazinam belongs to 2,6- dinitroanilines, is a kind of protective fungicide.Fluazinam is for interlinkage spore
Category, Botrytis, Botrytis cinerea category, Phytophthora, Plasmopara, Sclerotinia and it is black to cultivate Pseudomonas bacterium highly effective, have concurrently excellent
Control food plant property mite class effect, also have to crucifer clubroot brilliance preventive effect, to the water as caused by rhizopus
Rice samping off also has good preventive effect.
Through retrieving the application currently without Chinese yam polysaccharide in terms of bactericide synergy.
The content of the invention
The technical problem to be solved in the present invention is a kind of bactericidal composition containing Chinese yam polysaccharide and fluazinam of offer, significantly
The activity of fluazinam is improved, chemical pesticide dosage is reduced, delays the resistance to the action of a drug of pathogen to produce.
The technical scheme that present invention solution above-mentioned technical problem is taken is as follows：
A kind of bactericidal composition containing Chinese yam polysaccharide and fluazinam, active component is made up of Chinese yam polysaccharide and fluazinam, institute
The weight ratio of the Chinese yam polysaccharide and fluazinam stated in the composition is 0.1-5:1.
Described Chinese yam polysaccharide and the weight ratio of fluazinam are preferably：0.3:1、0.5:1 or 3:1.
Described bactericidal composition is prepared into wettable powder, water dispersible granules or granule.
Described bactericidal composition is used to prevent and treat crop in cruciferae clubroot and solanaceous crops late blight.
The beneficial effect that the present invention possesses is：Chinese yam polysaccharide and fluazinam are 0.1-5 according to weight ratio:1 is compounded, tool
There is an obvious synergistic function, the inhibitory action very little of Chinese yam polysaccharide for fungi in itself, its increasing mainly as fluazinam
Agent is imitated, inhibitory activity of the fluazinam to germ can be significantly improved, the two mixture can also reduce making for chemical agent fluazinam
With, so that cost is reduced, the residual of reduction chemical agent.
Embodiment
Technical scheme and beneficial effect are described further with reference to specific embodiment, it is of the present invention
Percentage be mass percent.
One indoor joint toxicity measuring test
In order to illustrate synergistic effect of the Chinese yam polysaccharide for fluazinam, the present invention calculates co-toxicity coefficient using the abundant methods of Sun Yun
(CTC) mixed effect is evaluated.
Toxicity index TI (A)=(EC50 of standard agent A EC50 ÷ A agent) × 100
Toxicity index TI (B)=(EC50 of standard agent A EC50 ÷ B agent) × 100
Actual toxicity index ATI (AB)=(A EC50 ÷ AB EC50) × 100
Percentage+TI (B) × B percentages in mixture of theoretical toxicity index TTI (AB)=TI (the A) × A in mixture
Number
Co-toxicity coefficient (CTC)=[mixture surveys toxicity index (ATI) ÷ mixtures theoretical toxicity index (TTI)] × 100
Evaluation criterion is：Co-toxicity coefficient >=120 show as synergistic effect；Co-toxicity coefficient≤80 show as antagonism；80<
Co-toxicity coefficient<120 show as summation action.
Test method：Reference《The parts of farm-chemical indoor determination test rule NY/T 1156.13-2008 the 13rd：Suppress
Late disease bacteria pilot blade method》, handled with the reagent spray for preparing respective concentration after potato healthy leaves, 24h, with preparation
Good spore suspension even spraying, cultivates 5-7d under suitable humiture and illumination condition, waits the sick leaf rate of blank control to reach
During to more than 50%, incidence is managed in classification investigation everywhere, calculates the EC of disease index and each medicament50, and according to the abundant methods of Sun Yun
Calculate the co-toxicity coefficient of mixture.
The Toxicity Determination result counted according to above-mentioned computational methods is as shown in the table：
As can be known from the above table, the weight ratio of fluazinam and Chinese yam polysaccharide is 1:During 0.1-5, its co-toxicity coefficient is all higher than 120, table
Reveal obvious synergistic function；And when weight ratio is 1:7、1:8 and 1:When 10, its co-toxicity coefficient is less than 80, shows
Obvious antagonism；When weight ratio is 1:When 15, simple summation action is only shown.
Two products prepare embodiment
The Chinese yam polysaccharide fluazinam wettable powder (3 of product embodiments 1 32%:1)
Chinese yam polysaccharide 24%, fluazinam 8%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 10%, lauryl sodium sulfate 8%, in vain
Carbon black 15%, bentonite supplies 100%.
Raw material weighing is carried out by above-mentioned formula, is crushed after being well mixed through airslide disintegrating mill, qualified i.e. obtained 32% is detected
Chinese yam polysaccharide fluazinam wettable powder.
The Chinese yam polysaccharide fluazinam water dispersible granules (0.3 of product embodiments 2 13%:1)
Chinese yam polysaccharide 3%, fluazinam 10%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 12%, sodium butylnaphthalenesulfonate 3%, starch
8%, sodium sulphate 3%, kaolin supplies 100%.
Raw material weighing is carried out by above-mentioned formula rate, after being crushed after being well mixed through airslide disintegrating mill, mediates, adds fluidisation
Bed granulation, drying and screening produces 13% Chinese yam polysaccharide fluazinam water dispersible granules.
The Chinese yam polysaccharide fluazinam granule (0.5 of product embodiments 3 1.5%:1)
Chinese yam polysaccharide 0.5%, fluazinam 1%, the formaldehyde condensation products 0.5% of methyl naphthalene sulfonic acid sodium, lauryl sodium sulfate
2%, polyvinyl alcohol 2%, kaolin supplies 100%.
Raw material weighing is carried out by above-mentioned formula rate, is well mixed, is crushed, is stirred after the wetting that adds water, then use
Screw extruder pelletizer is granulated, and 1.5% Chinese yam polysaccharide fluazinam granule is produced after drying and screening.
Reference product embodiment 1
3% Chinese yam polysaccharide wettable powder：Chinese yam polysaccharide 3%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 2%, dodecyl sulphur
Sour sodium 1%, white carbon 8%, bentonite supplies 100%.Preparation method is with product embodiments 1.
Reference product embodiment 2
1% fluazinam wettable powder：Fluazinam 1%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt 2%, lauryl sodium sulfate
1%, white carbon 8%, bentonite supplies 100%.Preparation method is with product embodiments 1.
Three crop field test examples
Test medicine：The said goods embodiment 1-3 respectively as test group 1-3, reference product embodiment 1-2 respectively as
Control group 1-2.Separately set blank control group.
Test method：Reference《The part of GB/T17980.34-2000 pesticide field efficacy medicine tests criterion (one) the 34th：Sterilization
Agent prevents and treats the late blight of potato》Test method.Second of spray pesticide after first time spray pesticide at the initial stage of a disease, 7d.Altogether
Twice, each processing sets 3 repetitions for dispenser, and each plot area is 66.7m2.Test site：Nanning.
Investigation method：5 sampling surveys, the every company of taking are carried out in 7d, 15d after second of dispenser in each cell respectively
Continuous 3 plants, look into whole blades, and classification record incidence, calculates disease index and preventive effect in units of blade；
Formula (1)：Disease index calculation formula：In formula (1)：X- disease indexs；Ni- disease at different levels
The number of sheets；I- is with respect to value of series；N- investigates total number of sheets.
Formula (2)：Prevention effect calculation formula：In formula (2)：P- prevention effects, unit
For percentage (%)；CK0Disease index before the dispenser of-blank district；CK1Disease index after the dispenser of-blank district；PT0- medicine
Disease index before agent treatment region dispenser；PT1Disease index after dispenser after-chemicals treatment.
And yield investigation is carried out when potato harvest, calculate per mu yield and rate of growth.As a result it is as shown in the table.
Prevent and treat the field control effectiveness test result of the late blight of potato
As seen from the above table, the composition of the invention containing Chinese yam polysaccharide and fluazinam be less than in active ingredient dosage or
In the case of comparison medicament, in terms of either preventing and treating the late blight of potato or volume increase, its effect is all more aobvious than comparison medicament
Write excellent.7d investigation results are shown after second of dispenser, prevention effect of the invention higher than fluazinam single dose 19.01% with
On；15d investigation results are shown after second of dispenser, and prevention effect of the invention is higher than fluazinam single dose by more than 20.24%；Harvest
When, effect of increasing production of the invention 14.53-27.04% higher than fluazinam single dose.
Claims (4)
1. a kind of bactericidal composition containing Chinese yam polysaccharide and fluazinam, it is characterised in that the active component in composition is by Chinese yam
Polysaccharide and fluazinam composition, the weight ratio of described Chinese yam polysaccharide and fluazinam in the composition is 0.1-5:1.
2. the bactericidal composition according to claim 1 containing Chinese yam polysaccharide and fluazinam, it is characterised in that described Chinese yam
The weight ratio of polysaccharide and fluazinam is：0.3:1、0.5:1 or 3:1.
3. the bactericidal composition according to claim 1 or 2 containing Chinese yam polysaccharide and fluazinam, it is characterised in that described
Bactericidal composition is prepared into wettable powder, water dispersible granules or granule.
4. the bactericidal composition according to claim 3 containing Chinese yam polysaccharide and fluazinam, it is characterised in that described sterilization
Composition is used to prevent and treat crop in cruciferae clubroot and solanaceous crops late blight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710298614.6A CN106962351A (en) | 2017-04-24 | 2017-04-24 | Bactericidal composition containing Chinese yam polysaccharide and fluazinam |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710298614.6A CN106962351A (en) | 2017-04-24 | 2017-04-24 | Bactericidal composition containing Chinese yam polysaccharide and fluazinam |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106962351A true CN106962351A (en) | 2017-07-21 |
Family
ID=59330608
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710298614.6A Withdrawn CN106962351A (en) | 2017-04-24 | 2017-04-24 | Bactericidal composition containing Chinese yam polysaccharide and fluazinam |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106962351A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103828812A (en) * | 2014-02-27 | 2014-06-04 | 海南正业中农高科股份有限公司 | Composition containing oligosaccharide and miazines bactericidal ingredients\ |
-
2017
- 2017-04-24 CN CN201710298614.6A patent/CN106962351A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103828812A (en) * | 2014-02-27 | 2014-06-04 | 海南正业中农高科股份有限公司 | Composition containing oligosaccharide and miazines bactericidal ingredients\ |
Non-Patent Citations (2)
| Title |
|---|
| 晓岚: "新杀菌剂氟啶胺的生物活性", 《农药译丛》 * |
| 杨柳: "山药多糖生物活性研究进展", 《农药译丛》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Aliabadi et al. | Antimicrobial activity of olive leaf aqueous extract | |
| CN104886070B (en) | Bactericidal composition and application thereof | |
| CN104872155B (en) | A kind of compound disinfectant preventing and treating rice blast | |
| CN106973907A (en) | A kind of composition pesticide containing copper 8-hydroxyquinolinate and kasugarnycin and its application | |
| CN107018979A (en) | Bactericidal composition containing Chinese yam polysaccharide and benzo alkene fluorine bacterium azoles | |
| CN104705324B (en) | A kind of bactericidal composition containing Zhongshengmycin and chitosan oligosaccharide | |
| CN109645010B (en) | Pharmaceutical composition for preventing and treating alternaria alternate and suspending agent thereof | |
| CN101700041A (en) | Bacillus subtilis and pyrimethanil compounded bactericide and application thereof | |
| CN107087612A (en) | Chinese yam polysaccharide as dithiocarbamate bactericide synergist application | |
| CN104351180B (en) | A kind of micro-capsule suspension for preventing and treating rice green smut | |
| CN106962351A (en) | Bactericidal composition containing Chinese yam polysaccharide and fluazinam | |
| CN107027758B (en) | Application of the Chinese yam polysaccharide as imidazoles bactericide synergist | |
| CN101703057B (en) | Agricultural germicide composite containing famoxadone | |
| CN107333783A (en) | Application of the composition containing methyl jasmonate in citrus bacterial canker disease | |
| CN107087610A (en) | Chinese yam polysaccharide as pyrazol acid amide bactericide synergist application | |
| CN102657212B (en) | Insecticidal composition and preparation thereof | |
| CN105746519A (en) | Pesticide composition containing fenoxanil and fungous proteoglycan | |
| CN104957149B (en) | A kind of bactericidal composition containing zoxamide and tiadinil | |
| CN104430361A (en) | Fungicidal composition containing JS399-19 and fluazinam | |
| CN106538604A (en) | A kind of bactericide containing isopyrazam | |
| CN107094767A (en) | Sterilization synergistic composition containing cyazofamid | |
| CN116019116B (en) | Pesticide powder and application method thereof | |
| CN107087614A (en) | A kind of bactericidal composition containing Chinese yam polysaccharide | |
| CN107079915A (en) | Composite bactericide and its application containing fluoxastrobin and jamaicin | |
| CN106962350A (en) | Bactericidal composition containing Chinese yam polysaccharide and fluopicolide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20170721 |
|
| WW01 | Invention patent application withdrawn after publication |