CN106954887A - A kind of preparation method of cigarette pineapple extract - Google Patents
A kind of preparation method of cigarette pineapple extract Download PDFInfo
- Publication number
- CN106954887A CN106954887A CN201610896558.1A CN201610896558A CN106954887A CN 106954887 A CN106954887 A CN 106954887A CN 201610896558 A CN201610896558 A CN 201610896558A CN 106954887 A CN106954887 A CN 106954887A
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- China
- Prior art keywords
- pineapple
- extract
- cigarette
- pineapple extract
- juice
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
- A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
- A24B15/302—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances by natural substances obtained from animals or plants
- A24B15/303—Plant extracts other than tobacco
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/10—Chemical features of tobacco products or tobacco substitutes
- A24B15/12—Chemical features of tobacco products or tobacco substitutes of reconstituted tobacco
Abstract
The present invention provides a kind of preparation method of cigarette pineapple extract, comprises the following steps：(1) pineapple is put into cold pressing expeller and carries out cold press, taken out juice and obtain pineapple juice, (2), using sodium alginate as carrier, immobilization beta glucuroide is made in embedding, and (3) use immobilization beta glucoside ferment treatment pineapple juice；(4) extract solution after ferment treatment is filtered, the pineapple extract solution after filtering is concentrated under reduced pressure, collected concentrate and obtain cigarette pineapple extract.Pineapple extract prepared by the present invention has unique pineapple fragrance, and with pleasant fragrant and sweet and fruity note.Pineapple extract is added in cigarette, with abundant cigarette perfume, increases fragrant and sweet and fruity note effect.
Description
Technical field
The present invention relates to the preparation field of essence spice for cigarette, a kind of preparation side of cigarette pineapple extract is specifically referred to
Method.
Background technology
In tobacco product, the fragrance and suction taste of tobacco are vital, and addition spices is the key in cigarette process
Link, is also to improve one of effective means of product style and raising cigarette flavor quality, some can be provided to consumer fragrant
Gas and the cigarette products for inhaling taste satisfaction.At present, the tobacco aromaticss added in China tobacco are to use conventional methods preparation mostly
, i.e., formed with natural extract monomer and synthon mixing preparation, although so obtained tobacco aromaticss market operation
Simply, but to there is fragrance dull, the shortcomings of improving property to tobacco not enough, and improve cigarette smoking quality DeGrain.
Pineapple (scientific name：Ananas comosus) it is one of famous tropical fruit (tree), it is pineapple family pineapple platymiscium, originates in
In the band of Amazon river one of South America Brazil, Paraguay, 16th century, its edible part was main by meat from Brazilian incoming China
Flower of the rachis, helical arrangement of increase in periphery is constituted, and spends generally shaky, the perianth sliver harbored surrounds a sky
Chamber, chamber it is built-in of wretched appearance stamen and style.Methyl mercaptomethyl propionate containing 3-, ethyl stearte, methyl caprylate, caproic acid in pineapple
The aroma substances such as methyl esters, 5- acetyl group-ethyl hexanoate, furfural, these aroma substances have the pineapple note of feature.And these are fragrant
Studies of The Aromatic Substances exists in glycoside link state form mostly, it is difficult to discharge.If obtained with customary preparation methods in spices, spices
The yield of aroma substance is relatively low so that cigarette perfume of the pineapple extract in cigarette be not obvious, exists so as to limit pineapple extract
Application in cigarette.
Beta-glucosidase (β-D-Glucosidase), also known as β-D-Glucose glycosides glucose hydrolysis enzyme, alias rough gentian two
Carbohydrase, cellobiase (cellobias) and amygdalase.It belongs to cellulose enzyme, in being cellulose decomposition enzyme system
Important composition composition, can hydrolyze glycoside, while discharging β-D-Glucose and fragrance component.
Immobilised enzymes (immobilized enzyme) is a kind of new technology grown up 1960s, is to use thing
The enzyme of reason or chemical method treatment of water-soluble be allowed to become water insoluble or be fixed on solid phase carrier but still with enzymatic activity
Enzyme derivative.Compared with resolvase, immobilised enzymes can with the long period repeatedly batch reactions and dress post successive reaction, so as to carry
The high service efficiency of enzyme, increases the yield of product, reduces cost；Immobilised enzymes easily divides with product after completion of the reaction
Open, simplify purifying technique, improve the quality of product.Therefore, it is widely used in waste water control, pharmaceutical engineering, chemical industry synthesis etc.
Field, but have no in cigarette is prepared with pineapple extract report.
The content of the invention
It is an object of the invention to provide a kind of preparation method of cigarette pineapple extract, the cigarette that it is prepared is carried with pineapple
Take that thing fragrance is more rich, quality is more preferable.
A kind of preparation method of cigarette pineapple extract, comprises the following steps：
(1) pineapple is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, take out juice and obtain pineapple juice；
(2) 0.1~0.3g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5),
The sodium alginate soln that 15~20mL mass concentrations are 2% is added, is stirred, it is 2% to add 20~30mL mass concentrations
Calcium chloride solution, 4 DEG C embedding 1~3h after, washed with cushioning liquid, obtain immobilized β-glucosidase, its bearing capacity be 10
~20U/g；
(3) take immobilized β-glucosidase handle pineapple juice, immobilized β-glucosidase solid consumption 12g/L~
20g/L, the pH value 3~6 of ferment treatment, 35 DEG C~40 DEG C of ferment treatment temperature, enzyme processing time 2h~5h, at 3 DEG C after the completion of processing
~4 DEG C of settlement treatment 48h~60h；
(4) extract solution after ferment treatment is filtered, obtains the pineapple extract solution of clarification, the pineapple extract solution after filtering is depressurized
Concentration, it is 1.10~1.20 to be concentrated into relative density, collects concentrate and obtains cigarette pineapple extract A；
(5) the pineapple extract A of step (4) is taken, propane diols is added, in 6~20Pa of pressure, feeding temperature is 60 DEG C~75
DEG C, charging rate is 5~30mL/min, and scraper plate rotating speed is to carry out molecular distillation under conditions of 60~130rpm, takes the light component to be
Pineapple extract；The percentage that the addition of wherein propane diols accounts for molecular distillation thing gross mass is 10%~30%.
Further, when the extract solution in the step (4) after ferment treatment is filtered, crossed with filter paper and filter off the miscellaneous of bulky grain
Matter, obtains the pineapple extract solution of clarification.
Further, when the step (4) is concentrated under reduced pressure, thickening temperature is 50~75 DEG C, and concentration pressure is 0.01~0.1
Individual atmospheric pressure.
Beneficial effects of the present invention：The present invention is handled in pineapple extract, hydrolysis pineapple with immobilized β-glucosidase
Aroma precursor material, discharge potential fragrance ingredient, greatly increase the extraction yield of the aromatic substance in pineapple, reduction life
Cost is produced, obtains that fragrance is more rich, the more preferable characteristic cigarette flavor of quality.Pineapple extract prepared by the present invention has only
Special pineapple fragrance, and with pleasant fragrant and sweet and fruity note.Pineapple extract is added in cigarette, with abundant cigarette
Fragrant, fragrant and sweet and fruity note the effect of increase.Present invention improves the quality of pineapple extract, pineapple extract is realized in volume
Application in cigarette.And this method can be easy to operate and automated by instrument precision control, and cost is more cheap, easy
Production conversion.
Pineapple juice, release 3- methyl mercaptomethyl propionates, ethyl stearte, octanoic acid are handled using immobilized β-glucosidase
The extraction yield of aromatic substance in methyl esters, methyl caproate, 5- acetyl group-ethyl hexanoate, furfural etc., increase pineapple, so as to drop
Low production cost.The cigarette pineapple extract of characteristic is prepared, this method technique is simple, low cost, extract yield height, fragrance-quality
It is good.Compared with free beta-glucosidase ferment treatment pineapple juice, after immobilization processing, it is not necessary to remove resolvase by UF membrane,
Production technology is simplified, is easy to industrialized production to convert；It is key odorant material 3- methyl mercaptomethyl propionates in pineapple extract, hard
Resin acid ethyl ester, methyl caprylate, methyl caproate, 5- acetyl group-ethyl hexanoate, furfural relative amount improve 28.40%, are added to
In cigarette, cigarette flavor more enriches, and fragrant and sweet rhythm is more obvious.
Embodiment
The relevant technical problem of the present invention is described in further detail with reference to embodiment.Embodiment 1
(1) pineapple is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, take out juice and obtain pineapple juice；
(2) 0.2g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5), added
18mL mass concentrations are 2% sodium alginate soln, are stirred, and add the calcium chloride solution that 26mL mass concentrations are 2%,
After 4 DEG C of embedding 1.5h, washed with cushioning liquid, obtain Immobilization in Sodium Alginate enzyme, its bearing capacity is 15U/g；
(3) immobilized β-glucosidase is taken to handle pineapple juice, immobilized β-glucosidase solid consumption 13g/L, enzyme
The pH value 3~4 of processing, 36 DEG C of ferment treatment temperature, enzyme processing time 2.5h, in 3 DEG C of settlement treatment 48h after the completion of processing；
(4) extract solution after ferment treatment is crossed to the impurity for filtering off bulky grain with filter paper, the pineapple extract solution of clarification is obtained, incited somebody to action
Pineapple extract solution after filter is concentrated under reduced pressure, and thickening temperature is 55 DEG C, and concentration pressure is concentrated into relative density for 0.03 atmospheric pressure
For 1.16, collect concentrate and obtain cigarette pineapple extract A；
(5) the pineapple extract A of step (4) is taken, propane diols is added, in pressure 6Pa, feeding temperature is 60, charging rate
For 5mL/min, scraper plate rotating speed is carries out molecular distillation under conditions of 60rpm, and it is pineapple extract to take light component；Wherein propane diols
Addition account for molecular distillation thing gross mass percentage be 10%.
Embodiment 2
(1) pineapple is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, take out juice and obtain pineapple juice；
(2) 0.15g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5), added
15mL mass concentrations are 2% sodium alginate soln, are stirred, and add the calcium chloride solution that 20mL mass concentrations are 2%,
After 4 DEG C of embedding 2h, washed with cushioning liquid, obtain immobilized β-glucosidase, its bearing capacity is 10U/g.
(3) immobilized β-glucosidase is taken to handle pineapple juice, immobilized β-glucosidase solid consumption 16g/L, enzyme
The pH value 4~5 of processing, 38 DEG C of ferment treatment temperature, enzyme processing time 3h, in 4 DEG C of settlement treatment 55h after the completion of processing；
(4) extract solution after ferment treatment is crossed to the impurity for filtering off bulky grain with filter paper, the pineapple extract solution of clarification is obtained, incited somebody to action
Pineapple extract solution after filter is concentrated under reduced pressure, and thickening temperature is 60 DEG C, and concentration pressure is concentrated into relative density for 0.10 atmospheric pressure
For 1.20, collect concentrate and obtain cigarette pineapple extract A；
(5) the pineapple extract A of step (4) is taken, propane diols is added, in pressure 16Pa, feeding temperature is 68 DEG C, charging speed
Spend for 14mL/min, scraper plate rotating speed is to carry out molecular distillation under conditions of 90rpm, and it is pineapple extract to take light component；Wherein third
The percentage that the addition of glycol accounts for molecular distillation thing gross mass is 22%.
Embodiment 3
(1) pineapple is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, take out juice and obtain pineapple juice；
(2) 0.3g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5), added
20mL mass concentrations are 2% sodium alginate soln, are stirred, and add the calcium chloride solution that 30mL mass concentrations are 2%,
After 4 DEG C of embedding 3h, washed with cushioning liquid, obtain immobilized β-glucosidase, its bearing capacity is 20U/g.
(3) immobilized β-glucosidase is taken to handle at pineapple juice, immobilized β-glucosidase solid consumption 20/L, enzyme
The pH value 5~6 of reason, 40 DEG C of ferment treatment temperature, enzyme processing time 5h, in 3 DEG C of settlement treatment 58h after the completion of processing；
(4) extract solution after ferment treatment is crossed to the impurity for filtering off bulky grain with filter paper, the pineapple extract solution of clarification is obtained, incited somebody to action
Pineapple extract solution after filter is concentrated under reduced pressure, and thickening temperature is 75 DEG C, and concentration pressure is concentrated into relative density for 0.08 atmospheric pressure
For 1.12, collect concentrate and obtain cigarette pineapple extract A；
(5) the pineapple extract A of step (4) is taken, propane diols is added, in pressure 20Pa, feeding temperature is 75 DEG C, charging speed
Spend for 30mL/min, scraper plate rotating speed is to carry out molecular distillation under conditions of 130rpm, and it is pineapple extract to take light component；Wherein third
The percentage that the addition of glycol accounts for molecular distillation thing gross mass is 30%.
The present invention handles pineapple juice using immobilized β-glucosidase, and it is identical with the present invention with other steps, and
Processing mode using free beta-glucosidase ferment treatment pineapple extract solution compares, as shown in table 1, is Different treatments pineapple
3- methyl mercaptomethyl propionates, ethyl stearte, methyl caprylate, methyl caproate, 5- acetyl group-ethyl hexanoate, furfural in extract
Total relative amount compares, as shown in table 2, and by the pineapple extract under Different treatments, the cigarette being added in cigarette is commented
Inhale result.
The key odorant material relative amount of table 1 compares
Sensory evaluation effect of the pineapple extract of the Different treatments of table 2 in cigarette
The preparation method of the present invention makes key odorant material 3- methyl mercaptomethyl propionates, stearic acid second in pineapple extract
Ester, methyl caprylate, methyl caproate, 5- acetyl group-ethyl hexanoate, furfural relative amount improve 28.40%, are added to volume
In cigarette, cigarette flavor more enriches, and fragrant and sweet rhythm is more obvious.Absolutely prove and prepared production using the extracting method of the present invention
Measure the more preferable pineapple extract of higher, quality.
The detailed description of specific embodiments of the present invention is the foregoing is only, the present invention is not limited with this, it is all at this
Any modification, equivalent and improvement for being made in the mentality of designing of invention etc., should be included in protection scope of the present invention it
It is interior.
Claims (3)
1. a kind of preparation method of cigarette pineapple extract, it is characterised in that comprise the following steps：
(1) pineapple is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, take out juice and obtain pineapple juice；
(2) 0.1~0.3g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5), added
15~20mL mass concentrations are 2% sodium alginate soln, are stirred, and add the chlorine that 20~30mL mass concentrations are 2%
Change calcium solution, 4 DEG C embedding 1~3h after, washed with cushioning liquid, obtain immobilized β-glucosidase, its bearing capacity be 10~
20U/g；
(3) take immobilized β-glucosidase handle pineapple juice, immobilized β-glucosidase solid consumption 12g/L~20g/L,
The pH value 3~6 of ferment treatment, 35 DEG C~40 DEG C of ferment treatment temperature, enzyme processing time 2h~5h is heavy at 3 DEG C~4 DEG C after the completion of processing
Drop processing 48h~60h；
(4) extract solution after ferment treatment is filtered, obtains the pineapple extract solution of clarification, the pineapple extract solution decompression after filtering is dense
Contracting, it is 1.10~1.20 to be concentrated into relative density, collects concentrate and obtains cigarette pineapple extract A；
(5) the pineapple extract A of step (4) is taken, propane diols is added, in 6~20Pa of pressure, feeding temperature is 60 DEG C~75 DEG C,
Charging rate is 5~30mL/min, and scraper plate rotating speed is carries out molecular distillation under conditions of 60~130rpm, and it is pineapple to take light component
Extract；The percentage that the addition of wherein propane diols accounts for molecular distillation thing gross mass is 10%~30%.
2. the preparation method of the cigarette pineapple extract described in claim 1 according to claim 1, it is characterised in that：
When extract solution in the step (4) after ferment treatment is filtered, the impurity for filtering off bulky grain is crossed with filter paper, the pineapple for obtaining clarification is extracted
Liquid.
3. the preparation method of cigarette pineapple extract according to claim 1, it is characterised in that：Step (4) decompression
During concentration, thickening temperature is 50~75 DEG C, and concentration pressure is 0.01~0.1 atmospheric pressure.
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|---|---|---|---|
| CN201610896558.1A CN106954887A (en) | 2016-10-13 | 2016-10-13 | A kind of preparation method of cigarette pineapple extract |
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| CN201610896558.1A CN106954887A (en) | 2016-10-13 | 2016-10-13 | A kind of preparation method of cigarette pineapple extract |
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Application publication date: 20170718 |