CN106947001A - A kind of extracting method of air bladder glycosaminoglycan and its application - Google Patents
A kind of extracting method of air bladder glycosaminoglycan and its application Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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Abstract
The present invention relates to a kind of extracting method of air bladder glycosaminoglycan and its application, S1：Enzymatic Extraction will add water and enzyme after air bladder drying and crushing, be that 7~9, temperature is that 3~5h is extracted under conditions of 40~60 DEG C in pH；Then go out enzyme activity, centrifuging and taking supernatant is glycosaminoglycan extract solution；S2：Extract solution obtained by ethanol precipitation concentration S1, it is 50~70% then to add ethanol to volume fraction, stirs and stands；S3：Sevag methods take off albumen and take gained after S2 standings to precipitate the GAG solution that is dissolved in water to obtain, and precipitation is gone in centrifugation；Then GAG solution is mixed with Sevag reagents and acutely shaken, the de- albumen of centrifugation is simultaneously dialysed with water；S4：Gained GAG solution after secondary precipitation concentration S3 dialysis, it is 50~70% to add ethanol to volume fraction, stirs and stands；S5：Precipitation organic solvent washing obtained by S4 is produced refined glycosaminoglycan by organic solvent washing after freeze-drying.The present invention, which extracts obtained air bladder glycosaminoglycan, has good moisture-absorbing moisture-keeping effect, can be applied in the functional food and cosmetics with moisture-keeping efficacy.
Description
Technical field
The present invention relates to animal polysaccharide extraction and separation technology field, in particular it relates to a kind of extraction of air bladder glycosaminoglycan
Method and its application.
Background technology
Air bladder, also known as fish maw or fish steep, and are colloid bladder, depth position control in water when being fish swimming.At me
State's Han dynasty just has record of the air bladder as medicinal material, and in the period Tang Dynasty, air bladder is as one of tribute, and, element equally celebrated for their achievements with bird's nest, shark's fin
There is the reputation of " ocean ginseng ".The traditional Chinese medical science think air bladder have nourishing yin and nourishing blood, invigorate the kidney and stop nocturnal emission, tender skin beauty treatment, anticancer the effects such as.China makees
For the big country of world's aquatic products first, possess abundant air bladder resource, but lack the research and development to efficacy factor in air bladder at present.
Forefathers' research show the Major Nutrient composition of air bladder for senior collagen, glycosaminoglycan and multivitamin and
The various trace elements such as calcium, zinc, iron, selenium, wherein GAG content are enriched, and account for the 8.9% of air bladder dry weight.At present, both at home and abroad
Research to air bladder is concentrated mainly in terms of Analysis of Nutritive Composition and collagen extraction, the separation to glycosaminoglycan in air bladder
Extract and its property research has no report.Glycosaminoglycan has been sent out as the bioactivator of rich content in marine organisms
Existing its shows stronger activity in terms of anticancer, antitumor, antiviral, antithrombotic, shows good application and development prospect,
It is expected to the raw material sources as new marine drug and functional health-care food.
Therefore, a kind of extracting method of air bladder glycosaminoglycan is studied to be significant.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of extracting method of air bladder glycosaminoglycan, sheet
Invention extracts air bladder glycosaminoglycan using enzymatic isolation method, and recovery rate is higher, extraction process flow is simple, rapidly and efficiently, cost it is relatively low.
Another object of the present invention is to provide said extracted method to extract obtained air bladder glycosaminoglycan as hygroscopic agent
Or application of the NMF in functional food or cosmetics.
To achieve the above object, the present invention is adopted the following technical scheme that：
A kind of extracting method of air bladder glycosaminoglycan, the extracting method comprises the following steps：
S1：Enzymatic Extraction
It will be added water and enzyme after air bladder drying and crushing, be that 7~9, temperature is that 3~5h is extracted under conditions of 40~60 DEG C in pH；Then
Go out enzyme activity, centrifuging and taking supernatant is glycosaminoglycan extract solution；
S2：Ethanol precipitation
Extract solution obtained by S1 is concentrated, it is 50~70% then to add ethanol to volume fraction, stirs and stands；
S3：Sevag methods take off albumen
Gained after S2 standings is taken to precipitate the GAG solution that is dissolved in water to obtain, precipitation is gone in centrifugation；Then by GAG solution with
Sevag reagents are mixed and acutely shaken, and the de- albumen of centrifugation is simultaneously dialysed with water；
S4：Secondary precipitation
Gained GAG solution after S3 dialysis is concentrated, it is 50~70% to add ethanol to volume fraction, stirs and stands；
S5：Organic solvent washing
By precipitation organic solvent washing obtained by S4, refined glycosaminoglycan is produced after freeze-drying.
The present invention is optimized using air bladder as raw material using Enzymatic Extraction glycosaminoglycan, and to its extraction process, improves sugar
The recovery rate of amine glycan.Method technological process that the present invention is provided is simple, rapidly and efficiently, cost is relatively low, raw materials used air bladder comes
Source is enriched, and is worth with higher medicine-food two-purpose and safe.The method that the present invention is provided extracts obtained glycosaminoglycan
In composition based on dermatan sulfate compound, containing a small amount of heparin, air bladder glycosaminoglycan has good moisture-absorbing moisture-keeping
Effect, can be applied in the functional food with moisture-keeping efficacy or cosmetics.
Preferably, in S1, the enzyme is the mixture of trypsase and withered grass neutral proteinase.
Preferably, in S1, the mass ratio of the trypsase and withered grass neutral proteinase is 3:5.
Preferably, in S1, the mass fraction of the enzyme is 4~8%.
Preferably, in S1, the solid-liquid ratio of the air bladder and water is 1:10~30.
In the S1 of the present invention, enzymolysis terminates to go out enzyme activity after heating 5~20min in 80~100 DEG C of water-bath, and in
10~30min is centrifuged under 5000r/min rotating speeds.
Preferably, in S2, extract solution obtained by S1 is concentrated into the 1/2~1/10 of initial volume.
Preferably, in S2 and S4, described stand is to stand 1~3 day in 4 DEG C.
Preferably, mixing time is 20min in S2.
Preferably, in S3, the mass fraction of the GAG solution is 1~3%.
Preferably, in S3, the volume ratio of the GAG solution and Sevag reagents is 3~5:1.
In the present invention, the Sevag reagents are that chloroform and n-butanol are 3~5 according to volume ratio:1 ratio mixing institute
.
In the S3 of the present invention, the time acutely shaken is 20min；The condition of the centrifugation is 3600r/min, from
Heart 10min；The dialysis time is 48h.
Preferably, in S4, concentration GAG solution to the 1/5~1/10 of initial volume.
In the S5 of the present invention, the organic solvent is absolute ethyl alcohol and/or acetone, is washed 2 times.
Said extracted method extracts obtained air bladder glycosaminoglycan as hygroscopic agent or NMF in functional food or cosmetic
Application in product.After tested, the moisture pick-up properties for the air bladder glycosaminoglycan that present invention extraction is obtained is better than chitosan, sodium alginate,
Performance of keeping humidity and glycerine, chitosan, sodium alginate quite, can be applied to the functional food with moisture-keeping efficacy or change
In cosmetic.
Compared with prior art, the present invention has the advantages that：
The present invention is optimized using air bladder as raw material using Enzymatic Extraction glycosaminoglycan, and to its extraction process, is improved osamine and is gathered
The recovery rate of sugar.The raw materials used air bladder abundance of method that the present invention is provided, is worth and pacifies with higher medicine-food two-purpose
Quan Xinggao.Composition in the glycosaminoglycan that the method extraction that the present invention is provided is obtained is contained based on dermatan sulfate compound
A small amount of heparin, air bladder glycosaminoglycan has good moisture-absorbing moisture-keeping effect, and after tested, the present invention extracts obtained air bladder osamine
The moisture pick-up properties of glycan is better than chitosan, sodium alginate, and performance of keeping humidity and glycerine, chitosan, sodium alginate are suitable；Can be by
It is applied in the functional food with moisture-keeping efficacy or cosmetics.The method technological process that the present invention is provided is simple, quick high
Effect, cost are relatively low, with larger application value.
Brief description of the drawings
Fig. 1 is the hydroscopicity contrast of air bladder glycosaminoglycan components and conventional NMF when the relative humidity that embodiment 1 is provided is 43%
Figure；
Fig. 2 is the hydroscopicity contrast of air bladder glycosaminoglycan components and conventional NMF when the relative humidity that embodiment 1 is provided is 81%
Figure；
Fig. 3 is the moisturizing rate comparison diagram of the air bladder glycosaminoglycan components that embodiment 1 is provided and conventional NMF；
Fig. 4 is the FT-IR spectrum figure for the air bladder glycosaminoglycan components that embodiment 1 is provided；
Fig. 5 is the cellulose acetate electrophoresis figure for the air bladder glycosaminoglycan components that embodiment 1 is provided.
Embodiment
With reference to detailed description drawings and Examples, the present invention is expanded on further.These embodiments are merely to illustrate this hair
Bright rather than limitation the scope of the present invention.The experimental method of unreceipted actual conditions in lower example embodiment, generally according to ability
Domain normal condition or the condition advised according to manufacturer.It is any that those skilled in the art is done on the basis of the present invention
The change and replacement of unsubstantiality belong to scope of the present invention.
The extraction of the air bladder glycosaminoglycan of embodiment 1
The air bladder powder 100g crushed is weighed, 1L distilled water is added, the addition of total enzyme is 1%（Trypsase and the neutral egg of withered grass
White enzyme ratio is 3:5）, pH8, temperature 50 C, extraction time 4h are digested, enzymolysis heats 10min in 100 DEG C of water-baths after terminating and gone out
Enzyme activity, centrifugation（5000r/min, 20min）It is glycosaminoglycan extract solution to take supernatant；Extract solution is concentrated into initial volume
After 1/3, it is 60% to be slowly added to ethanol to volume fraction, persistently stirs and 4 DEG C of standing 24h are placed in after 20min；Precipitation is taken, with distillation
Water is configured to the GAG solution that mass fraction is 3%, centrifugation（6000r/min, 10 min）Precipitation is gone, chloroform is used：N-butanol
=4:1 mixture, by GAG solution：Sevage reagent=4:1 ratio acutely shakes 20 min, centrifugation（3600r/min,
10 min）De- albumen, 48 h of distilled water dialysis；Solution after dialysis is concentrated into the 1/10 of initial volume, ethanol is added to body
Fraction is 60%, and being persistently placed in 4 DEG C after stirring 20min stands 1 day；The precipitation absolute ethyl alcohol and third that secondary alcohol precipitation is obtained
Ketone is freeze-dried after alternately washing 2 times respectively, obtains glycosaminoglycan moisture-absorption humectant.
The extraction of the air bladder glycosaminoglycan of embodiment 2
The air bladder powder 100g crushed is weighed, 2L distilled water is added, the addition of total enzyme is 6%（Trypsase and the neutral egg of withered grass
White enzyme ratio is 3:5）, pH8, temperature 60 C, extraction time 4h are digested, enzymolysis heats 10min in 100 DEG C of water-baths after terminating and gone out
Enzyme activity, centrifugation（5000r/min, 20min）It is glycosaminoglycan extract solution to take supernatant；Extract solution is concentrated into initial volume
After 1/3, it is 60% to be slowly added to ethanol to volume fraction, persistently stirs and 4 DEG C of standing 24h are placed in after 20min；Precipitation is taken, with distillation
Water is configured to the GAG solution that mass fraction is 3%, centrifugation（6000r/min, 10 min）Precipitation is gone, chloroform is used：N-butanol
=4:1 mixture, by GAG solution：Sevage reagent=4:1 ratio acutely shakes 20 min, centrifugation（3600r/min,
10 min）De- albumen, 48 h of distilled water dialysis；Solution after dialysis is concentrated into the 1/10 of initial volume, ethanol is added to body
Fraction is 60%, and being persistently placed in 4 DEG C after stirring 20min stands 1 day；The precipitation absolute ethyl alcohol and third that secondary alcohol precipitation is obtained
Ketone is freeze-dried after alternately washing 2 times respectively, obtains glycosaminoglycan moisture-absorption humectant.
The extraction of the air bladder glycosaminoglycan of embodiment 3
The air bladder powder 100g crushed is weighed, 2L distilled water is added, the addition of total enzyme is 7%（Trypsase and the neutral egg of withered grass
White enzyme ratio is 3:5）, enzymolysis pH7.5, temperature 50 C, extraction time 4h to be digested and heat 10min in 100 DEG C of water-baths after terminating
Go out enzyme activity, centrifugation（5000r/min, 20min）It is glycosaminoglycan extract solution to take supernatant；Extract solution is concentrated into initial volume
1/3 after, be slowly added to ethanol to volume fraction be 60%, persistently stir 20min after be placed in 4 DEG C standing 24h；Precipitation is taken, with steaming
Distilled water is configured to the GAG solution that mass fraction is 3%, centrifugation（6000r/min, 10 min）Precipitation is gone, chloroform is used：Positive fourth
Alcohol=4:1 mixture, by GAG solution：Sevage reagent=4:1 ratio acutely shakes 20 min, centrifugation（3600r/
Min, 10 min）De- albumen, 48 h of distilled water dialysis；Solution after dialysis is concentrated into the 1/10 of initial volume, ethanol is added
It is 60% to volume fraction, being persistently placed in 4 DEG C after stirring 20min stands 1 day；The precipitation absolute ethyl alcohol that secondary alcohol precipitation is obtained
It is freeze-dried after alternately being washed 2 times respectively with acetone, obtains glycosaminoglycan moisture-absorption humectant.
The extraction of the air bladder glycosaminoglycan of embodiment 4
The air bladder powder 100g crushed is weighed, 2L distilled water is added, the addition of total enzyme is 6%（Trypsase and the neutral egg of withered grass
White enzyme ratio is 3:5）, enzymolysis pH8.5,55 DEG C of temperature, extraction time 4h to be digested and heat 10min in 100 DEG C of water-baths after terminating
Go out enzyme activity, centrifugation（5000r/min, 20min）It is glycosaminoglycan extract solution to take supernatant；Extract solution is concentrated into initial volume
1/3 after, be slowly added to ethanol to volume fraction be 60%, persistently stir 20min after be placed in 4 DEG C standing 24h；Precipitation is taken, with steaming
Distilled water is configured to the GAG solution that mass fraction is 3%, centrifugation（6000r/min, 10 min）Precipitation is gone, chloroform is used：Positive fourth
Alcohol=4:1 mixture, by GAG solution：Sevage reagent=4:1 ratio acutely shakes 20 min, centrifugation（3600r/
Min, 10 min）De- albumen, 48 h of distilled water dialysis；Solution after dialysis is concentrated into the 1/10 of initial volume, ethanol is added
It is 60% to volume fraction, being persistently placed in 4 DEG C after stirring 20min stands 1 day；The precipitation absolute ethyl alcohol that secondary alcohol precipitation is obtained
It is freeze-dried after alternately being washed 2 times respectively with acetone, obtains glycosaminoglycan moisture-absorption humectant.
The recovery rate that the various embodiments described above extract obtained glycosaminoglycan, extraction side are tested and calculated using A Lixin blue laws
Method is as follows, extracts result and see the table below 1.
0.10g liquaemin standard items accurately are weighed, 100 mL are settled to distilled water, 1mg/mL liquaemin mark is configured to
Quasi- solution.To 15% H3PO4With 2%H2SO4Mixed solution in add 0.110 g A Li Xinlan, fully dissolving after be settled to 100
mL。
0,20,40,60,80,100 μ L heparin sodium standard solutions are drawn respectively in test tube, and moisturizing to 100 uL is added
1.5 mL A Li Xinlan dyeing liquors are dyed, and are stored at room temperature 10 min, and the colorimetric estimation absorbance at the nm of wavelength 490 is obtained
Standard curve is returned
The equation is returned to be：Y=0.002 X-0.0011, R2=0.9999；Y is absorbance, X be heparin sodium content/（µg/mL）.
The formula of air bladder glycosaminoglycan recovery rate is as follows：
Air bladder glycosaminoglycan recovery rate=100 × glycosaminoglycan quality（g）/ air bladder silty amount（g）
The recovery rate for the glycosaminoglycan that the extracting method of the embodiment 1~4 of table 1 is extracted
Performance test
（1）The moisture-absorbing moisture-keeping performance test of air bladder glycosaminoglycan
The moisture-absorbing moisture-keeping performance that the various embodiments described above extract obtained glycosaminoglycan is tested and evaluated, it is intended to for air bladder sugar
Amine glycan is applied to provide certain theoretical foundation in functional food and cosmetics as NMF.
1. the moisture pick-up properties test of air bladder glycosaminoglycan
The hygroscopicity of air bladder glycosaminoglycan is determined to be carried out under the conditions of constant temperature and humidity, and specific method of testing is as follows：
Saturated solution of potassium carbonate and ammonium sulfate saturated solution are respectively placed in drier, it is 20 DEG C to control environment temperature, is made
Relative humidity is respectively 43% and 81% environment.Air bladder glycosaminoglycan, sodium alginate, chitosan and several specification identicals are claimed
Measuring bottle is dried to constant mass under the conditions of 50 DEG C.Accurately weigh 0.1 g samples to be placed in measuring cup, gathered with glycerine, shell
Sugar, sodium alginate are control, measuring cup opening are placed in the drier that relative humidity is respectively 43% and 81%, hermetically drying
Device, its mass change is weighed after placing 3,6,12,24,36,48,60,72 h.Test result is shown in Fig. 1 and Fig. 2 respectively.
The computing formula of hydroscopicity is as follows：
Hydroscopicity=100 ×（Mt- M0）/ Mt,
In formula：M0 is quality/g of each sample before moisture absorption；
Mt is quality/g of each sample after moisture absorption different time.
2. the performance of keeping humidity test of air bladder glycosaminoglycan
The moisture retention of air bladder glycosaminoglycan is determined to be selected under the dry environment of constant temperature and carried out, and specific method is as follows：
It is 20 DEG C to control environment temperature, and 200 g discoloration silica gel is added in drier bottom.By polysaccharide, reference substance and several specifications
Small beaker for 20 mL is placed in drying box, 50 DEG C of dryings to constant mass.It is accurate weigh air bladder glycosaminoglycan, glycerine,
Sodium alginate, chitosan each 0.1 g, 10 mL that add water respectively are configured to the solution that mass concentration is 1 mg/m L.Prepare what is completed
The equal opening of solution is placed in constant-temperature enclosed silica gel drier, and its matter is weighed after placing 4,12,24,36,48,60,72,84,96 h
Amount change.Test result is shown in Fig. 3.
Moisturizing rate=100 × Mt/ M0,
In formula：M0 is quality/g of each sample before moisture absorption；
Mt is quality/g of each sample after moisture absorption different time.
After tested, the present invention extracts the moisture pick-up properties of obtained air bladder glycosaminoglycan better than chitosan, sodium alginate, moisturizing
Performance and glycerine, chitosan, sodium alginate are suitable.
（2）Infra-red sepectrometry analyzes the structure of air bladder glycosaminoglycan
The infrared scan spectrum of air bladder glycosaminoglycan moisturizing hygroscopic agent is shown in Fig. 4, and air bladder glycosaminoglycan is in 3406.223cm-1Place goes out
Now wider peak, illustrates there is-the OH of carbohydrate；In 2920.171cm-1It is by-CH that moderate strength absworption peak, which occurs, in place3C-H pairs
Claim what stretching vibration was produced；In 2850cm-1It is by-CH that weak absworption peak, which occurs, in place3C-H antisymmetric stretching vibrations produce；
1652.963cm-1There is absorption at place, illustrates the C=O stretching vibrations that there is amide groups；In 1413.795cm-1The small peak at place is by sugar
The C-H of class becomes what angular oscillation was produced；In 1226.703cm-1The weak absorbing peak that place occurs is drawn by the S=O stretching vibrations of sulfonyl
Rise, the feature of glycosaminoglycan is met above.In addition, 1045.298cm-1It is the ether in pyranose ring that strong absworption peak, which occurs, in place
Caused by the stretching vibration of key and the change angular distortion of O-H keys；840.9475cm-1There is absworption peak, 890-900 cm in place-1Section
Without substantially absorption, show air bladder glycosaminoglycan based on α type pyranosides.
The infrared absorption light of the moisture-absorption humectant extracted from air bladder and dermatan sulfate standard items obtained by the present invention
Spectrogram coincide substantially（Can be referring to《Extraction, purifying, structure and the activity research of fish-skin dermatan sulfate》；2008；Liao Wenjuan）, therefore
Assert that the extract of the present invention is main based on dermatan sulfate class compound.
（3）Cellulose acetate electrophoresis
By air bladder glycosaminoglycan and heparin after purification（HP）, dermatan sulfate（DS）Standard items are each configured to 3 mg/mL
The aqueous solution.Cellulose acetate film is placed in the mol/L formic acid of 0.1 mol/L pyridines -0.47（pH3.0）Buffer solution soaks 30 min
Afterwards, point sample, the min of electrophoresis 20,0.5% is immersed in by film under 7 mA electric currents（Mass fraction）Contaminated in alcian blue acetum
The min of color 20, then with 2% acetum decolouring, 30 min.
Fig. 5 is the cellulose acetate electrophoresis figure for the air bladder glycosaminoglycan components that embodiment 1 is provided, and the band of left figure is
The histogram of air bladder glycosaminoglycan, right figure is the histogram of heparin and dermatan sulfate, and wherein heparin is upper, and dermatan sulfate exists
Under.As seen from Figure 5, it is main based on dermatan sulfate in air bladder glycosaminoglycan, containing a small amount of heparin, in sulfuric acid skin
The underside of element also has a light band, it is necessary to further research；The result of this and air bladder glycosaminoglycan infrared spectrum
It is the same, illustrates air bladder glycosaminoglycan really using dermatan sulfate as main species.
Claims (10)
1. a kind of extracting method of air bladder glycosaminoglycan, it is characterised in that the extracting method comprises the following steps：
S1：Enzymatic Extraction
It will be added water and enzyme after air bladder drying and crushing, be that 7~9, temperature is that 3~5h is extracted under conditions of 40~60 DEG C in pH；Then
Go out enzyme activity, centrifuging and taking supernatant is glycosaminoglycan extract solution；
S2：Ethanol precipitation
Extract solution obtained by S1 is concentrated, it is 50~70% then to add ethanol to volume fraction, stirs and stands；
S3：Sevag methods take off albumen
Gained after S2 standings is taken to precipitate the GAG solution that is dissolved in water to obtain, precipitation is gone in centrifugation；Then by GAG solution with
Sevag reagents are mixed and acutely shaken, and the de- albumen of centrifugation is simultaneously dialysed with water；
S4：Secondary precipitation
Gained GAG solution after S3 dialysis is concentrated, it is 50~70% to add ethanol to volume fraction, stirs and stands；
S5：Organic solvent washing
By precipitation organic solvent washing obtained by S4, refined glycosaminoglycan is produced after freeze-drying.
2. the extracting method of air bladder glycosaminoglycan according to claim 1, it is characterised in that in S1, the enzyme is tryptose
The mixture of enzyme and withered grass neutral proteinase.
3. the extracting method of air bladder glycosaminoglycan according to claim 2, it is characterised in that in the trypsase and withered grass
Property protease mass ratio be 3:5.
4. the extracting method of air bladder glycosaminoglycan according to claim 1, it is characterised in that the mass fraction of the enzyme is 4
~8%.
5. the extracting method of air bladder glycosaminoglycan according to claim 1, it is characterised in that in S1, the air bladder and water
Solid-liquid ratio is 1:10~30.
6. the extracting method of air bladder glycosaminoglycan according to claim 1, it is characterised in that in S2, by extract solution obtained by S1
It is concentrated into the 1/2~1/10 of initial volume.
7. the extracting method of air bladder glycosaminoglycan according to claim 1, it is characterised in that in S2 and S4, the standing is
1~3 day is stood in 4 DEG C.
8. the extracting method of air bladder glycosaminoglycan according to claim 1, it is characterised in that in S3, the glycosaminoglycan is molten
The mass fraction of liquid is 1~3%.
9. the extracting method of air bladder glycosaminoglycan according to claim 1, it is characterised in that in S3, the glycosaminoglycan is molten
The volume ratio of liquid and Sevag reagents is 3~5:1.
10. any extracting method of claim 1~9 is extracted obtained air bladder glycosaminoglycan and existed as hygroscopic agent or NMF
Application in functional food or cosmetics.
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| CN111011567A (en) * | 2019-11-28 | 2020-04-17 | 程红 | Tabletting candy with memory improving function and preparation method thereof |
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Application publication date: 20170714 |