CN106930739A - A kind of method that endogenous microbes single well stimulation recovers the oil - Google Patents
A kind of method that endogenous microbes single well stimulation recovers the oil Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 30
- 230000000638 stimulation Effects 0.000 title claims abstract description 19
- 241000894006 Bacteria Species 0.000 claims abstract description 122
- 238000002474 experimental method Methods 0.000 claims abstract description 83
- 239000003129 oil well Substances 0.000 claims abstract description 79
- 239000003921 oil Substances 0.000 claims abstract description 48
- 239000010779 crude oil Substances 0.000 claims abstract description 46
- 238000012360 testing method Methods 0.000 claims abstract description 38
- 238000004945 emulsification Methods 0.000 claims abstract description 34
- 229930195733 hydrocarbon Natural products 0.000 claims abstract description 34
- 150000002430 hydrocarbons Chemical class 0.000 claims abstract description 34
- 239000004215 Carbon black (E152) Substances 0.000 claims abstract description 30
- 230000003647 oxidation Effects 0.000 claims abstract description 29
- 238000007254 oxidation reaction Methods 0.000 claims abstract description 29
- 230000004913 activation Effects 0.000 claims abstract description 27
- 238000011156 evaluation Methods 0.000 claims abstract description 19
- 238000012216 screening Methods 0.000 claims abstract description 15
- 230000000694 effects Effects 0.000 claims abstract description 9
- 238000002347 injection Methods 0.000 claims description 59
- 239000007924 injection Substances 0.000 claims description 59
- 239000012190 activator Substances 0.000 claims description 56
- 235000019198 oils Nutrition 0.000 claims description 46
- 238000004519 manufacturing process Methods 0.000 claims description 35
- 239000003795 chemical substances by application Substances 0.000 claims description 17
- 239000007836 KH2PO4 Substances 0.000 claims description 10
- 239000008398 formation water Substances 0.000 claims description 10
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 10
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 9
- 230000035699 permeability Effects 0.000 claims description 9
- 244000063299 Bacillus subtilis Species 0.000 claims description 7
- 241000588624 Acinetobacter calcoaceticus Species 0.000 claims description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 229940084030 carboxymethylcellulose calcium Drugs 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 235000019774 Rice Bran oil Nutrition 0.000 claims description 3
- 230000001590 oxidative effect Effects 0.000 claims description 3
- 239000008165 rice bran oil Substances 0.000 claims description 3
- 238000012549 training Methods 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 210000000481 breast Anatomy 0.000 claims 2
- 230000004064 dysfunction Effects 0.000 claims 1
- 239000002207 metabolite Substances 0.000 abstract description 4
- 230000000813 microbial effect Effects 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 3
- 239000002131 composite material Substances 0.000 abstract 1
- 238000011084 recovery Methods 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 241001037822 Bacillus bacterium Species 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000003876 biosurfactant Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 241000193764 Brevibacillus brevis Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000004308 accommodation Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000003209 petroleum derivative Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
Classifications
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Geology (AREA)
- Mining & Mineral Resources (AREA)
- Physics & Mathematics (AREA)
- Environmental & Geological Engineering (AREA)
- Fluid Mechanics (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Geochemistry & Mineralogy (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to Microbial Enhanced Oil Recovery field, and in particular to a kind of method that endogenous microbes single well stimulation recovers the oil, comprise the following steps：Test the screening of oil well；Hydrocarbon oxidation function bacterium activates experiment of handling up；Emulsification of crude oil function bacterium activates experiment of handling up；Aerogenesis function bacterium activates experiment of handling up；The evaluation of field test and test effect.The present invention is using the hydrocarbon oxidation function bacterium in activation experiment oil well, emulsification of crude oil function bacterium and aerogenesis function bacterium, and comprehensive function using above-mentioned functions bacterium metabolite reaches the purpose that effectively improves oil well output.The characteristics of invention has process is simple, specific aim, good workable and field test results, the term of validity averagely increased day by day oily 3t, the input-output ratio of being more than more than 1 more than 24 months, individual well:4.5.Therefore, the composite can be widely applied to improve oil well output field test in.
Description
Technical field
The present invention relates to a kind of microbe oil production method, the side that more particularly to a kind of endogenous microbes single well stimulation recovers the oil
Method.
Background technology
Microbial single well stimulation is directed to the specified strain or activator of producing well injection screening, by bacterium itself and its
The effect of metabolite, processes well wellbore and near wellbore formation, plays improvement crude oil property, reduces crude oil flow resistance, improves
Oil well output.The features such as microbial single well stimulation has ground small investment, simple to operate, instant effect, especially to well wellbore and
Play a part of to improve crude oil property, reduce crude oil flow resistance, improve crude oil production timeliness near wellbore zone.
In the prior art, proprietary term " oil production method with microbial single well stimulation ", application number " 200610030442.6 "
Patent selects the microorganism fungus kind Brevibacillus brevis HT and Bacillus cercus HP with petroleum hydrocarbon as sole carbon source, according to ground
HT and HP bacterium are first distinguished fermented and cultured, then carry out by proper proportion by the present situation of flow, the method that injection is concentrated using cementing truck
Prepare, injected from oil well oil jacket annular space, bacterium solution injection finishes rear injected clear water, and the bacterium solution in oil jacket annular space is headed into stratum.
The technology is related to the problems such as bacteria selection, the production of bacterium solution, transport of bacterium solution, and adaptation of the strain for screening to stratum
Property it is poor, in face of stratum high-temperature, high pressure, high salinity extreme environment, so as to have impact on scene implementation result and scene push away
Wide application.
Notification number " CN103291267 B ", " a kind of utilization reservoir endogenous micro-organisms improve the side of oil well output to patent name
Method ", discloses a kind of method that utilization reservoir endogenous micro-organisms improve oil well output, and the method is comprised the following steps：Experiment oil
The screening of well；The screening of activator；The determination of activator injection amount；Closed-in time determines；Field test.The shortcoming of the method exists
In：(1) accommodation of oil reservoir is small, 30 DEG C of 80 DEG C of ＜ reservoir temperatures ＜, formation water salinity ＜ 80000mg/L, viscosity of crude
10~1000mPas of scope, and be more than with viscosity of crude more than 80000mg/L more than 80 DEG C, formation water salinity for temperature
The oil reservoir of 1000mPas is not suitable for the method；(2) using the biosurfactant production, generation gas and product tested in oil well
Any one or more can not effectively improve the well yield of heavy crude reservoir in three kinds of microorganisms of biopolymer；(3) three class work(
The activator injection technics comparing of energy bacterium (biosurfactant production, generation gas and generation polymer bacterium) is single, it is impossible to
Classification activation is realized, therefore have impact on field test results to a certain extent.
The content of the invention
Present invention aim to overcome that above-mentioned the deficiencies in the prior art, and a kind of endogenous microbes single well stimulation is provided and is recovered the oil
Method, the present invention using the hydrocarbon oxidation function bacterium in activation experiment oil well, emulsification of crude oil function bacterium and aerogenesis function bacterium, and
The purpose for effectively improving oil well output is reached using the comprehensive function of above-mentioned functions bacterium metabolite, the invention has technique letter
The good advantage of list, specific aim, workable and field test results.
The invention discloses a kind of method that endogenous microbes single well stimulation recovers the oil, it is characterised in that the method is specifically wrapped
Include following steps：
(1) screening of oil well is tested
The screening of oil well is tested, it is necessary to meet two conditions：(1) 90 DEG C of reservoir temperature ＜, formation water salinity ＜
100000mg/L, in-place permeability ＞ 100 × 10-3μm2, viscosity of crude it is thick less than 5000mPa.s, moisture content ＜ 98%, oil reservoir
Degree is more than 2m；(2) three kinds of hydrocarbon oxidation function bacterium, emulsification of crude oil function bacterium and aerogenesis function bacterium are contained in experiment produced liquid in oil well
Functional microorganism, and bacterium is dense is all higher than 1.0 × 102Individual/mL.
(2) hydrocarbon oxidation function bacterium activates experiment of handling up
Using high pressure pump truck to the activator that hydrocarbon oxidation function bacterium is injected in experiment oil well, closed after the completion of activator injection
Well culture, opening well and making production after 15~30d of culture.
(3) emulsification of crude oil function bacterium activates experiment of handling up
Using high pressure pump truck to the activator that emulsification of crude oil function bacterium is injected in experiment oil well, closed after the completion of activator injection
Well culture, opening well and making production after 5~10d of culture.
(4) aerogenesis function bacterium activates experiment of handling up
Using high pressure pump truck to the activator that aerogenesis function bacterium is injected in experiment oil well, closing well training after the completion of activator injection
Support, opening well and making production after 30~60d of culture.
(5) evaluation of field test and test effect
The evaluation of field test results is carried out after experiment oil wells in field off-test, evaluation index averagely increases day by day including individual well
Oil mass, the term of validity and input-output ratio.
Wherein, described hydrocarbon oxidation function bacterium is pseudomonad or acinetobacter calcoaceticus；Described hydrocarbon oxidation function bacterium
Activation agent prescription is 2~5wt% of glucose, NH40.5~1.0wt% of Cl, K2HPO40.1~0.2wt%；Described hydro carbons oxygen
The injection rate for changing the activator of function bacterium is relevant with the viscosity of crude of experiment oil well：3000mPa.s≤viscosity of crude ﹤
5000mPa.s, every meter of core intersection injects 15~20m3；1000mPa.s≤viscosity of crude ﹤ 3000mPa.s, every meter of core intersection
10~15m of injection3；Viscosity of crude ﹤ 1000mPa.s, every meter of core intersection injects 5~10m3。
The initial time that the 2nd described round emulsification of crude oil function bacterium activates experiment of handling up is the hydrocarbon oxidation of the 1st round
Function bacterium activation handle up experiment opening well and making production when oil well daily oil production when being equal to corresponding during oil well daily oil production before field test
Between.
Described emulsification of crude oil function bacterium is ground bacillus or bacillus subtilis；Described emulsification of crude oil function bacterium
Activation agent prescription is 1~2wt% of carboxymethylcellulose calcium, 0.2~0.5wt% of peptone, KH2PO40.1~0.2wt%；Described
The injection rate of the activator of emulsification of crude oil function bacterium is relevant with the moisture content of experiment oil well：90%≤moisture content ﹤ 98%, per rice bran oil
Thickness degree injects 5~10m3；80%≤moisture content ﹤ 90%, every meter of core intersection injects 10~15m3；Moisture content ﹤ 80%, every meter
Core intersection injects 15~20m3。
The initial time that the 3rd described round aerogenesis function bacterium activates experiment of handling up is the 2nd round emulsification of crude oil function bacterium
Activation handle up experiment opening well and making production when oil well daily oil production corresponding time when being equal to oil well daily oil production before field test.
Described aerogenesis function bacterium is methanogen or aerobacteria；The activation agent prescription of described aerogenesis function bacterium is complete
2~3wt% of flour, 0.5~0.8wt% of Dried Corn Steep Liquor Powder, KH2PO40.1~0.2wt%；The activation of described aerogenesis function bacterium
The injection rate of agent is relevant with the core intersection of experiment oil well：Core intersection >=10m, activator injection amount is 50~100m3；5m≤
Core intersection ﹤ 10m, activator injection amount is 20~50m3；2m ﹤ core intersection ﹤ 5m, every meter of core intersection injects 10~20m3。
The characteristics of present invention is for experiment oil well selects three techniques of handling up of round.The hydro carbons of the 1st round is carried out first
Oxidative function bacterium activates experiment of handling up, to the hydro carbons in the activator activation oil well that hydrocarbon oxidation function bacterium is injected in experiment oil well
Oxidative function bacterium, the viscosity of crude oil is reduced by hydrocarbon oxidation function bacterium, so as to improve the stream of crude oil to the degradation of crude oil
Dynamic performance；Next emulsification of crude oil function bacterium for carrying out the 2nd round activates experiment of handling up, to injection emulsification of crude oil work(in experiment oil well
Emulsification of crude oil function bacterium in the activator activation oil well of energy bacterium, is dropped by the emulsification of emulsification of crude oil function bacterium metabolite
Low oil water interfacial tension, so as to reduce the oil and water mobility ratio of oil well fluid；Finally carry out the aerogenesis function bacterium activation of the 3rd round
Handle up experiment, to the aerogenesis function bacterium in the activator activation oil well that aerogenesis function bacterium is injected in experiment oil well, aerogenesis function bacterium
Metabolism produces the gases such as biogas such as methane and carbon dioxide, and on the one hand the biogas of generation improve the energy of experiment oil well
Amount, dissolving of the another aspect biogas in crude oil reduces the viscosity of crude oil.Reached by the experiment of handling up of above three round
To the purpose for increasing substantially experiment oil well output, experiment oil well individual well averagely increases day by day oily more than 3t.
The present invention has the following advantages that and beneficial effect compared with prior art：
(1) using the endogenous microbes for activating itself presence in oil well, without artificially adding other inoculating microbes, so that
Field test cost is saved；
(2) present invention is with strong points, and different activation agent prescriptions are selected for different endogenous functional microorganisms, effectively carries
High activation effect and field test results；
(3) the interior activator of present invention injection is nutriment, therefore will not be made to stratum generation injury and to environment
Into pollution；
(4) oil reservoir of the invention is applied widely, is both adapted to the oil reservoir of high permeability, and high temperature and high salt oil deposit is adapted to again；
(5) the characteristics of invention has process is simple, workable and field test results are good, the oil well term of validity is more than
24 months, individual well averagely increase day by day oil more than 3t, input-output ratio be more than 1:4.5.
Specific implementation method
This invention is described in further detail with reference to embodiment：
Embodiment 1：
Experiment oil well H12Overview：65 DEG C of reservoir temperature, formation water salinity 16532mg/L, permeability 550 × 10-3μm2、
Viscosity of crude 1258mPa.s, moisture content 95.3%, core intersection 4.5m, oil well day oil-producing 3.2t before experiment；Endogenous microbes point
Analysis result is 1.0 × 10 for pseudomonad bacterium is dense3It is 1.1 × 10 that individual/mL, ground bacillus bacterium are dense3Individual/mL, methanogen bacterium are dense
It is 5.0 × 102Individual/mL.Implement specific steps of the invention in the well：
(1) screening of oil well is tested
Experiment oil well H1265 DEG C of reservoir temperature, formation water salinity 16532mg/L, permeability 550 × 10-3μm2, crude oil
Viscosity 1258mPa.s, moisture content 95.3%, core intersection 4.5m；Endogenous microbes analysis result is 1.0 for pseudomonad bacterium is dense
×103It is 1.1 × 10 that individual/mL, ground bacillus bacterium are dense3It is 5.0 × 10 that individual/mL, methanogen bacterium are dense2Individual/mL.Meet this hair
Bright screening criteria.
(2) hydrocarbon oxidation function bacterium activates experiment of handling up
Using high pressure pump truck to the activator that hydrocarbon oxidation function bacterium is injected in experiment oil well, closed after the completion of activator injection
Well culture, opening well and making production after culture 15d.
Hydrocarbon oxidation function bacterium is pseudomonad, and the activation agent prescription of pseudomonad is glucose 2wt%, NH4Cl
0.5wt%, K2HPO40.1wt%；The injection rate of the activator of pseudomonad is every meter of core intersection injection 11m3, injection rate is
49.5m3。
(3) emulsification of crude oil function bacterium activates experiment of handling up
Initial time is that the hydrocarbon oxidation function bacterium of the 1st round activates the 250d after experiment opening well and making production of handling up, using height
Press pump car cultivates 5d to the activator that emulsification of crude oil function bacterium is injected in experiment oil well, closing well culture after the completion of activator injection
Opening well and making production afterwards.
Emulsification of crude oil function bacterium is ground bacillus, the activation agent prescription of ground bacillus is carboxymethylcellulose calcium 1wt%,
Peptone 0.2wt%, KH2PO40.1wt%；The injection rate of the activator of ground bacillus is every meter of core intersection injection 8m3,
Injection rate is 36m3。
(4) aerogenesis function bacterium activates experiment of handling up
Initial time is that the emulsification of crude oil function bacterium of the 2nd round activates the 320d after experiment opening well and making production of handling up, using height
Press pump car is opened to the activator that aerogenesis function bacterium is injected in experiment oil well, closing well culture after the completion of activator injection after culture 30d
Well is produced.
Aerogenesis function bacterium is methanogen, and the activation agent prescription of methanogen is wholemeal 2wt%, Dried Corn Steep Liquor Powder
0.5wt%, KH2PO40.1wt%；The injection rate of the activator of methanogen is every meter of core intersection injection 18m3, injection rate is
81m3。
(5) evaluation of field test and test effect
The evaluation of field test results is carried out after experiment oil wells in field off-test, evaluation index averagely increases day by day including individual well
Oil mass, the term of validity and input-output ratio.
Field test results evaluation result：The oil well term of validity 32 months, oily 3.5t, the input-output ratio of averagely increasing day by day are 1:
5.3。
Embodiment 2：
Experiment oil well H15Overview：72 DEG C of reservoir temperature, formation water salinity 21320mg/L, permeability 900 × 10-3μm2、
Viscosity of crude 1865mPa.s, moisture content 92.5%, core intersection 6.0m, oil well day oil-producing 4.3t before experiment；Endogenous microbes point
Analysis result is 2.0 × 10 for pseudomonad bacterium is dense3It is 1.0 × 10 that individual/mL, ground bacillus bacterium are dense3Individual/mL, aerobacteria bacterium are dense
It is 2.0 × 103Individual/mL.Implement specific steps of the invention in the well：
(1) screening of oil well is tested
Experiment oil well H1572 DEG C of reservoir temperature, formation water salinity 21320mg/L, permeability 900 × 10-3μm2, crude oil
Viscosity 1865mPa.s, moisture content 92.5%, core intersection 6.0m；Endogenous microbes analysis result is 2.0 for pseudomonad bacterium is dense
×103It is 1.0 × 10 that individual/mL, ground bacillus bacterium are dense3It is 2.0 × 10 that individual/mL, aerobacteria bacterium are dense3Individual/mL.Meet this hair
Bright screening criteria.
(2) hydrocarbon oxidation function bacterium activates experiment of handling up
Using high pressure pump truck to the activator that hydrocarbon oxidation function bacterium is injected in experiment oil well, closed after the completion of activator injection
Well culture, opening well and making production after culture 20d.
Hydrocarbon oxidation function bacterium is pseudomonad, and the activation agent prescription of pseudomonad is glucose 4wt%, NH4Cl
0.8wt%, K2HPO40.15wt%；The injection rate of the activator of pseudomonad is every meter of core intersection injection 12m3, injection rate
It is 72.0m3。
(3) emulsification of crude oil function bacterium activates experiment of handling up
Initial time is that the hydrocarbon oxidation function bacterium of the 1st round activates the 280d after experiment opening well and making production of handling up, using height
Press pump car cultivates 8d to the activator that emulsification of crude oil function bacterium is injected in experiment oil well, closing well culture after the completion of activator injection
Opening well and making production afterwards.
Emulsification of crude oil function bacterium is ground bacillus, and the activation agent prescription of ground bacillus is carboxymethylcellulose calcium
1.5wt%, peptone 0.3wt%, KH2PO40.15wt%；The injection rate of the activator of ground bacillus is every meter of core intersection
Injection 6m3, injection rate is 36m3。
(4) aerogenesis function bacterium activates experiment of handling up
Initial time is that the emulsification of crude oil function bacterium of the 2nd round activates the 350d after experiment opening well and making production of handling up, using height
Press pump car is opened to the activator that aerogenesis function bacterium is injected in experiment oil well, closing well culture after the completion of activator injection after culture 45d
Well is produced.
Aerogenesis function bacterium is aerobacteria, and the activation agent prescription of aerobacteria is wholemeal 2.5wt%, Dried Corn Steep Liquor Powder
0.6wt%, KH2PO40.15wt%；The injection rate of the activator of aerobacteria is every meter of core intersection injection 25m3, injection rate
It is 150m3。
(5) evaluation of field test and test effect
The evaluation of field test results is carried out after experiment oil wells in field off-test, evaluation index averagely increases day by day including individual well
Oil mass, the term of validity and input-output ratio.
Field test results evaluation result：The oil well term of validity 35 months, oily 5.4t, the input-output ratio of averagely increasing day by day are 1:
5.7。
Embodiment 3：
Experiment oil well H32Overview：80 DEG C of reservoir temperature, formation water salinity 35856mg/L, permeability 1200 × 10-3μm2、
Viscosity of crude 2560mPa.s, moisture content 96.5%, core intersection 4.0m, oil well day oil-producing 2.8t before experiment；Endogenous microbes point
Analysis result is 1.1 × 10 for acinetobacter calcoaceticus bacterium is dense3It is 3.0 × 10 that individual/mL, bacillus subtilis bacterium are dense3Individual/mL, aerobacteria bacterium
Dense is 2.0 × 102Individual/mL.Implement specific steps of the invention in the well：
(1) screening of oil well is tested
Experiment oil well H3280 DEG C of reservoir temperature, formation water salinity 35856mg/L, permeability 1200 × 10-3μm2, it is former
Oil viscosity 2560mPa.s, moisture content 96.5%, core intersection 4.0m；Endogenous microbes analysis result is that acinetobacter calcoaceticus bacterium is dense
1.1×103It is 3.0 × 10 that individual/mL, bacillus subtilis bacterium are dense3It is 2.0 × 10 that individual/mL, aerobacteria bacterium are dense2Individual/mL.Meet
Screening criteria of the invention.
(2) hydrocarbon oxidation function bacterium activates experiment of handling up
Using high pressure pump truck to the activator that hydrocarbon oxidation function bacterium is injected in experiment oil well, closed after the completion of activator injection
Well culture, opening well and making production after culture 30d.
Hydrocarbon oxidation function bacterium is acinetobacter calcoaceticus, and the activation agent prescription of acinetobacter calcoaceticus is glucose 5wt%, NH4Cl
1.0wt%, K2HPO40.2wt%；The injection rate of the activator of hydrocarbon oxidation function bacterium is every meter of core intersection injection 14m3, note
Enter amount for 56m3。
(3) emulsification of crude oil function bacterium activates experiment of handling up
Initial time is that the hydrocarbon oxidation function bacterium of the 1st round activates the 275d after experiment opening well and making production of handling up, using height
Press pump car cultivates 10d to the activator that emulsification of crude oil function bacterium is injected in experiment oil well, closing well culture after the completion of activator injection
Opening well and making production afterwards.
Emulsification of crude oil function bacterium is bacillus subtilis, and the activation agent prescription of bacillus subtilis is carboxymethylcellulose calcium
2wt%, peptone 0.5wt%, KH2PO40.2wt%；The injection rate of the activator of bacillus subtilis is every meter of core intersection
Injection 8.5m3, injection rate is 34m3。
(4) aerogenesis function bacterium activates experiment of handling up
Initial time is that the emulsification of crude oil function bacterium of the 2nd round activates the 320d after experiment opening well and making production of handling up, using height
Press pump car is opened to the activator that aerogenesis function bacterium is injected in experiment oil well, closing well culture after the completion of activator injection after culture 60d
Well is produced.
Aerogenesis function bacterium is aerobacteria, and the activation agent prescription of aerobacteria is wholemeal 3wt%, Dried Corn Steep Liquor Powder
0.8wt%, KH2PO40.2wt%；The injection rate of the activator of aerobacteria is every meter of core intersection injection 17.5m3, injection rate
It is 70m3。
(5) evaluation of field test and test effect
The evaluation of field test results is carried out after experiment oil wells in field off-test, evaluation index averagely increases day by day including individual well
Oil mass, the term of validity and input-output ratio.
Field test results evaluation result：The oil well term of validity 30 months, oily 3.2t, the input-output ratio of averagely increasing day by day are 1:
5.0。
Claims (10)
1. a kind of method that endogenous microbes single well stimulation recovers the oil, it is characterised in that the method specifically includes following steps：
(1) screening of oil well is tested
The screening of oil well is tested, it is necessary to meet two conditions：(1) 90 DEG C of reservoir temperature ＜, formation water salinity ＜ 100000mg/
L, in-place permeability ＞ 100 × 10-3μm2, viscosity of crude less than 5000mPa.s, moisture content ＜ 98%, core intersection be more than 2m；
(2) hydrocarbon oxidation function bacterium, the three kinds of micro- lifes of function of emulsification of crude oil function bacterium and aerogenesis function bacterium are contained in experiment produced liquid in oil well
Thing, and bacterium is dense is all higher than 1.0 × 102Individual/mL；
(2) hydrocarbon oxidation function bacterium activates experiment of handling up
Using high pressure pump truck to the activator that hydrocarbon oxidation function bacterium is injected in experiment oil well, closing well training after the completion of activator injection
Support, opening well and making production after 15~30d of culture；
(3) emulsification of crude oil function bacterium activates experiment of handling up
Using high pressure pump truck to the activator that emulsification of crude oil function bacterium is injected in experiment oil well, closing well training after the completion of activator injection
Support, opening well and making production after 5~10d of culture；
(4) aerogenesis function bacterium activates experiment of handling up
Using high pressure pump truck to experiment oil well in inject aerogenesis function bacterium activator, closing well culture after the completion of activator injection,
Opening well and making production after 30~60d of culture；
(5) evaluation of field test and test effect
Carry out the evaluation of field test results after experiment oil wells in field off-test, evaluation index averagely increases day by day oil including individual well
Amount, the term of validity and input-output ratio.
2. the method that endogenous microbes single well stimulation according to claim 1 recovers the oil, it is characterised in that described hydro carbons oxygen
It is pseudomonad or acinetobacter calcoaceticus to change function bacterium.
3. the method that endogenous microbes single well stimulation according to claim 1 and 2 recovers the oil, it is characterised in that described hydrocarbon
The activation agent prescription of class oxidative function bacterium is 2~5wt% of glucose, NH40.5~1.0wt% of Cl, K2HPO40.1~
0.2wt%；The injection rate of the activator of described hydrocarbon oxidation function bacterium is relevant with the viscosity of crude of experiment oil well：
3000mPa.s≤viscosity of crude ﹤ 5000mPa.s, every meter of core intersection injects 15~20m3；1000mPa.s≤viscosity of crude ﹤
3000mPa.s, every meter of core intersection injects 10~15m3；Viscosity of crude ﹤ 1000mPa.s, every meter of core intersection injects 5~10m3。
4. the method that endogenous microbes single well stimulation according to claim 1 recovers the oil, it is characterised in that the 2nd described wheel
The initial time that secondary emulsification of crude oil function bacterium activates experiment of handling up is that the hydrocarbon oxidation function bacterium of the 1st round activates experiment opening of handling up
Corresponding time when oil well daily oil production is equal to oil well daily oil production before field test when well is produced.
5. the method that endogenous microbes single well stimulation according to claim 1 recovers the oil, it is characterised in that described crude oil breast
It is ground bacillus or bacillus subtilis to change function bacterium.
6. the method that endogenous microbes single well stimulation according to claim 5 recovers the oil, it is characterised in that described crude oil breast
The activation agent prescription for changing function bacterium is 1~2wt% of carboxymethylcellulose calcium, 0.2~0.5wt% of peptone, KH2PO40.1~
0.2wt%.
7. the method that the endogenous microbes single well stimulation according to claim 5 or 6 recovers the oil, it is characterised in that described original
The injection rate of the activator of oily emulsification function bacterium is relevant with the moisture content of experiment oil well：90%≤moisture content ﹤ 98%, the layer per rice bran oil
Thickness injects 5~10m3；80%≤moisture content ﹤ 90%, every meter of core intersection injects 10~15m3；Moisture content ﹤ 80%, per rice bran oil
Thickness degree injects 15~20m3。
8. the method that endogenous microbes single well stimulation according to claim 1 recovers the oil, it is characterised in that the 3rd described wheel
Secondary aerogenesis function bacterium activates the initial time of experiment of handling up for the 2nd round emulsification of crude oil function bacterium activates experiment opening well and making production of handling up
When oil well daily oil production corresponding time when being equal to oil well daily oil production before field test.
9. the method that endogenous microbes single well stimulation according to claim 8 recovers the oil, it is characterised in that described aerogenesis work(
Energy bacterium is methanogen or aerobacteria.
10. the method that endogenous microbes single well stimulation according to claim 8 or claim 9 recovers the oil, it is characterised in that described product
The activation agent prescription of airway dysfunction bacterium is 2~3wt% of wholemeal, 0.5~0.8wt% of Dried Corn Steep Liquor Powder, KH2PO40.1~
0.2wt%；The injection rate of the activator of described aerogenesis function bacterium is relevant with the core intersection of experiment oil well：Core intersection >=
10m, activator injection amount is 50~100m3；5m≤core intersection ﹤ 10m, activator injection amount is 20~50m3；2m ﹤ oil reservoirs are thick
Degree ﹤ 5m, every meter of core intersection injects 10~20m3。
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