CN110566168A - method for activating endogenous microorganism to enhance oil recovery or pollution treatment by injecting Dietzia - Google Patents
method for activating endogenous microorganism to enhance oil recovery or pollution treatment by injecting Dietzia Download PDFInfo
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- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
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- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
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Abstract
The invention discloses a method for activating endogenous microorganisms to enhance oil recovery or pollution control by injecting Dietzia. The invention provides a method for improving the processing efficiency of a sample to be processed, which is to activate the activity of endogenous microorganisms in the sample to be processed by using Dietzia microorganisms so as to improve the processing efficiency of the sample to be processed; the sample to be processed is crude oil, the water body polluted by the crude oil or the soil polluted by the crude oil; the improvement of the treatment efficiency of the sample to be treated is the improvement of the recovery rate of crude oil or the improvement of the pollution treatment effect of the crude oil polluted water body or the crude oil polluted soil. Experiments prove that the method for improving the crude oil recovery rate by injecting Dietzia microorganisms into the oil reservoir to directionally activate functional microorganisms in the endogenous microbial community of the oil reservoir is found.
Description
Technical Field
The invention belongs to the field of biotechnology, and relates to a method for activating endogenous microorganisms by injecting Dietzia to enhance oil recovery or pollution treatment.
Background
The main oil field development of China is in the later stage of water injection development, high water-cut oil reservoirs and heavy oil reservoirs become main oil reservoirs, and crude oil reserves are huge but difficult to exploit. The microorganism enhanced oil recovery technology (MEOR) has the advantages of low cost, environmental friendliness and the like, and has wide application prospects in the development of oil reservoirs. The microbial oil recovery mainly utilizes the biodegradation of microbes and metabolites thereof to reduce the viscosity and the freezing point of crude oil and improve the fluidity of the crude oil, thereby improving the yield and the recovery ratio of the crude oil. Currently, microbial oil recovery is divided into exogenous microbial oil recovery and endogenous microbial oil recovery technologies. The endogenous microbial oil recovery technology is characterized in that inherent microbial communities in an oil reservoir are directly utilized, and a nutrition activating agent is added to activate beneficial endogenous microbial communities in the oil reservoir, so that metabolites are generated to achieve the purposes of improving the oil reservoir and increasing the crude oil recovery ratio. Exogenous microorganism oil recovery is realized by adding exogenous microorganisms into an oil reservoir to exert the oil recovery function and enhance the recovery ratio of crude oil. Traditionally, exogenous microbial oil recovery and endogenous microbial oil recovery have been considered as two distinct techniques of fracturing.
correspondingly, two modes of playing the role of the microorganism exist in the process of pollution treatment by utilizing the microorganism, namely a bioaugmentation process and a biostimulation process. The biological strengthening process technology is that external microorganisms are added into the polluted environment to play a role in pollution treatment; the biostimulation process technology is to add the nutrition or favorable conditions of the microorganisms into the polluted environment and exert the function of the indigenous microorganisms to treat the pollution. Conventionally, the bioaugmentation process technique and the biostimulation process technique are also considered as two techniques of cleaving each other.
Disclosure of Invention
In order to improve the crude oil recovery ratio of high water-cut oil reservoirs and heavy oil reservoirs and the treatment of crude oil polluted soil or water, the invention provides the following technical scheme:
An object of the present invention is to provide a method for improving the efficiency of processing a sample to be processed.
The method provided by the invention is to utilize Dietzia microorganisms to activate the activity of endogenous microorganisms in a sample to be processed so as to improve the processing efficiency of the sample to be processed;
The sample to be processed is crude oil, the water body polluted by the crude oil or the soil polluted by the crude oil;
The improvement of the treatment efficiency of the sample to be treated is the improvement of the recovery rate of crude oil or the improvement of the pollution treatment effect of the crude oil polluted water body or the crude oil polluted soil.
In the method, the activity of using the Dietzia microorganisms to activate endogenous microorganisms in the sample to be treated is to add the Dietzia microorganisms to the sample to be treated; in an embodiment of the invention, a Dietzia microorganism is injected into an oil well.
in the above method, the enhanced oil recovery is achieved by emulsifying the crude oil or by promoting the flow of the crude oil;
Or the pollution treatment effect of the crude oil polluted water or the crude oil polluted soil is improved when the water quality index is improved or the content of organic pollutants is reduced.
In the above method, the endogenous microorganisms in the sample to be treated include bacillus, pseudomonas and/or proteus.
The above endogenous microorganisms further include Dietzia (Dietzia), Paenibacillus (Bacteroides), Microbacterium (Microbacterium) and/or Alcaligenes (Alcaligenes).
The endogenous microorganism comprises one or any combination of the above.
the amount of Dietzia microorganisms added to the sample to be treated is related to the thickness of oil layer, action range, water content, construction period, daily liquid yield and indoor evaluation coefficient, and the amount of injected bacteria is 150-1000m3And the concentration of the bacterial liquid is more than or equal to 108cfu/mL。
In the embodiment of the invention, in the indoor physical model flooding experiment, the injection quantity according to the volume of 1 model pore space (model pipe volume (pi) 25)2300/4) porosity by gasometry) into a microbial infusion;
The preparation method of the microorganism injection liquid comprises the following steps:
And (3) microbial liquid: inoculating the activated microorganism strains of each group (column 5 of Table 4) in LB culture medium, performing shaking culture at 30 deg.C and 150rpm for 24 hr to obtain microorganism solution of each group (with a concentration of 10 or more)8cfu/mL)；
the microorganism bacterium liquid in the experimental group G is obtained by mixing a bacterium liquid of the bacterium of the addict.
Nutrient solution: 4g/L NH4NO3；4g/L KH2PO4；5.68g/L Na2HPO42g/L of peptone and 1g/L of yeast powder, and the balance of injected water;
Microorganism injection liquid: adding the above groups of microbial solutions into the nutrient solution to make the volume percentage of the microbial solution in the nutrient solution be 1% (after dilution, the thallus is propagated to make its thallus concentration be greater than or equal to 10)6cfu/mL)。
In an embodiment of the invention, in the oilfield microbial stimulation operation:
The exogenous microorganism Dietzia maris CGMCC NO.1.6332 bacterial liquid (the bacterial concentration is more than or equal to 10)8cfu/mL), Dietzia psychralcaliphila CGMCC NO.1.6149 bacterial liquid (bacterial concentration is more than or equal to 10)8cfu/mL) and Dietzia natronolimnaae CGMCC NO.1.6147 bacterial liquid (bacterial concentration is more than or equal to 10)8cfu/mL) is prepared into a mixed microbial inoculum according to the cfu ratio of 1:1:1, and then the mixed microbial inoculum and the nutrient solution are diluted according to the volume ratio of 1:99 to obtain diluted bacterial solution. The amount of the diluted bacterial liquid is 500m3Injecting the single well into crude oil of an oil reservoir below the ground, and carrying out well opening production after stewing for 2 months.
The application of the Dietzia microorganisms in the method in improving the recovery ratio of the crude oil is also within the protection scope of the invention; wherein enhancing recovery of the crude oil occurs by emulsifying the crude oil or promoting flow of the crude oil;
The microorganisms in the crude oil include bacillus, pseudomonas and/or proteus (Proteobacteria).
The Dietzia microorganisms in the method are also applied to improving the treatment effect of the pollution of the crude oil polluted water body or improving the treatment effect of the pollution of the crude oil polluted soil; wherein the improvement of the pollution treatment effect of the crude oil polluted water or the crude oil polluted soil is reflected in that the water quality index becomes better or the content of organic pollutants is reduced. The microorganisms in the crude oil contaminated water body include bacillus, pseudomonas and/or proteus (Proteobacteria).
The crude oil is particularly from a medium-high water-cut oil reservoir (the water content is more than 40%) or a heavy oil reservoir (the viscosity is more than 1000mPa & s at the oil reservoir temperature).
Experiments prove that the method for improving the crude oil recovery rate by injecting the exogenous microorganism Dietzia into the oil reservoir to activate the functional microorganism in the endogenous microbial community of the oil reservoir.
Drawings
FIG. 1 shows the results of pH values after culturing 6 experimental groups and control groups to which exogenous microorganisms were added.
FIG. 2 shows the surface tension results of 6 experimental groups and control groups to which exogenous microorganisms were added after culture.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1 enhanced oil recovery by Dietzia activation of endogenous microorganisms
The crude oil sample is produced from 862m oil well produced liquid of 6-month Jilin oil field in 2017, the stratum temperature is 45 ℃, the water content of the oil reservoir is 90%, the crude oil belongs to the high water-content oil reservoir, the crude oil is naturally stood for one day, and then the crude oil in the crude oil is taken out in a sterile manner. The physical and chemical properties of the crude oil are measured as follows: density: 867.3kg m-3(ii) a Viscosity, 10963 mPas (measurement at 30 ℃); the freezing point is 35 ℃; the water content is 1.03%; crude oil four components: 48.85% of saturated hydrocarbon, 18.83% of aromatic hydrocarbon, 12.96% of non-hydrocarbon and 21.36% of colloid and asphaltene. The originally sterilized crude oil was subjected to cell collection by centrifugation and DNA extraction for amplification, but because of the extremely low concentration, no information on its microbial community was obtained, but its endogenous microorganisms were known to include Bacillus, Pseudomonas, Dietzia and Paenibacillus.
identification of exogenous microorganism Dietzia for activating endogenous microorganism
1) exogenous microbial strains
the following 6 microorganisms were selected: CGMCC represents a microorganism purchased from institute of microbiology, national academy of sciences
a1-1, Acinetobacter beiLeizienae CGMCC NO. 1.12683;
A1-2, Dietzia maris CGMCC NO. 1.6332;
A1-3, Dietzia alcaliphila CGMCC NO. 1.6149;
A1-4, Acinetobacter baumannii CGMCC NO. 1.10395;
A1-5, Pseudomonas kunmingensis CGMCC NO. 1.16086;
A1-6, Dietzia natronolimnaea CGMCC NO. 1.6147.
2) Inoculating to crude oil for culture
(1) Activating the 6 strains from a glycerol tube into a flat plate, picking monoclonal cells, transferring the monoclonal cells into a test tube for culture, and finally inoculating the cells into a flask containing 300mL of LB (500mL of triangular flask) for culture to obtain a seed solution.
(2) After the seed liquid is cultured to a logarithmic growth phase, centrifuging at 5000rpm and 4 deg.C for 10min to collect cells, washing with inorganic salt culture medium for 3 times, starving for 30min, mixing with inorganic salt culture medium, and detecting the thallus concentration (OD600) at 600nm wavelength to OD600 ═ 0.10 with ultraviolet spectrophotometer to obtain culture solution (cfu value greater than or equal to 10)7cfu/mL)。
(3) The 6 culture liquids obtained in (2) above were transferred to mineral salt medium containing crude oil at a concentration of 1.0% (by volume) to obtain 6 experimental groups A1-1 to A1-6.
The formula of the inorganic salt culture medium (MSM) is as follows: NaCl,5 g; NH (NH)4H2PO4,4g；(NH4)2SO4,1g；MgSO4·7H2O,0.2g；KNO3,3g；K2HPO41 g; adding H2O is increased to 1 liter, and the pH is adjusted to 7.4;
The inorganic salt culture medium containing crude oil is prepared by adding crude oil serving as a carbon source into the inorganic salt culture medium, and the mass volume percentage of the crude oil in the inorganic salt culture medium containing the crude oil is 2% (the unit is g: l).
3 flasks without any microorganisms were set as a control.
Protected from light, and cultured at 150rpm for 30 days on a shaker at 30 ℃.
3) detection of
(1) Crude oil emulsification detection
The products (emulsions) obtained after 30-day incubation of the above 2)6 experimental groups and control groups were tested for OD600 by UV spectrophotometer, and the results are shown in Table 1:
Table 1 shows the OD600 detection of the product (emulsion) after 30 days of culture
| Experiment number | A1-1 | A1-2 | A1-3 | A1-4 | A1-5 | A1-6 | Control group |
| OD600 | 33.62 | 61.23 | 68.79 | 26.56 | 28.28 | 72.76 | 0.06 |
It can be seen that the turbidity of the liquid was increased in 6 experimental groups compared to the OD600 before inoculation of 0.10, in which the dirts were significantly changed from both the acinetobacter and pseudomonas experimental groups, and the control group was not significantly changed; it was shown that the crude oils of the 3 groups of Dietzia groups A1-2, A1-3 and A1-6 emulsified more than the other groups.
(2) determination of surface tension, pH and composition of remaining oil
referring to the results of pH values of the above-mentioned 2)6 experimental groups and the control group as shown in FIG. 1, it can be seen that the pH value of the 6 experimental groups is rapidly lowered in about 10 days and then at a relatively low level until the end of the experiment compared to the control group, wherein the pH value of the genus Dietzia reaches the lowest value preferentially to that of the genus Acinetobacter and Pseudomonas, indicating that the genus Dietzia can preferentially degrade crude oil and produce small-molecule organic acids to lower the pH value.
The surface tension of the above 2)6 experimental groups and the control group was measured using a spinning drop surface interfacial tension meter, and as a result, as shown in fig. 2, it can be seen that the surface tension of the 6 experimental groups was rapidly decreased for about 10 days and then at a relatively low level until the end of the experiment compared to the control group, and wherein the interfacial tension of the genus dietzia is lower relative to the genera acinetobacter and pseudomonas, indicating that the dietzia can better emulsify the crude oil.
The viscosities of the reaction products of the above-mentioned 2)6 experimental groups and the control group were measured by a continuously variable digital viscometer (SNB-1, Shanghai precision instruments Co., Ltd.), and as a result, as shown in Table 2, the viscosities of the crude oils were decreased and the fluidity of the crude oils was increased in all of the 6 experimental groups as compared with the control group, wherein 3 groups of Dietzia genus, A1-2, A1-3 and A1-6, showed more significant decrease in viscosity as compared with the experimental groups of Acinetobacter and Pseudomonas.
Table 2 crude oil viscosity of the reaction product
The components of the remaining oil after 30 days of culture in the above 2)6 experimental groups and the control group were measured according to the SY/T5119-2008 rock soluble organic matter and crude oil group component analysis standards, and the results were as follows, and the four component analysis results were as follows: compared with the control group, the saturated hydrocarbon components of the A1-3 to A1-6 experimental groups are all reduced, and the non-hydrocarbon content is reduced and the aromatic hydrocarbon, colloid and asphaltene components are improved to a certain extent in the A1-1, A1-4 and A1-5 experimental groups.
(3) Detection of activated endogenous microbial communities
And (3) uniformly taking 20ml of liquid out of 100ml of culture products of the 6 experimental groups and the control group in the step 2), centrifuging at 8000rpm for 10min, collecting supernatant, sending to Megi biology company in Shanghai for sequencing analysis to obtain the abundance and variety of each microorganism species, and realizing the detection of the microorganism communities.
in the control group, cells were collected by centrifugation, and DNA was extracted and amplified, but information on the microbial community was not obtained because of the extremely low concentration.
In the A1-1 experimental group, Bacillus (73.6% abundance), Pseudomonas (22.1%) and Dietzia (2.1%) were detected, and hence Acinetobacter bereziae CGMCC NO.1.12683 activated Bacillus, Pseudomonas and Dietzia in the oil reservoir.
In the experimental group a1-2, Pseudomonas (52.1%), Proteobacteria (17.6%), Bacillus (21.2%) and Dietzia (3.1%) were detected, and thus Dietzia maris cgmccno.1.6332 activated Pseudomonas, Proteobacteria, Bacillus and Dietzia in the reservoir.
in the experimental group A1-3, Pseudomonas (52.4%), Bacillus (27.6%), Paenibacillus (4.4%), Microbacterium (2.5%) and Alcaligenes (1.6%) were detected, and thus Dietzia psahralcaliphila CGMCC NO.1.6149 activated Pseudomonas, Bacillus, Paenibacillus, Microbacterium and Alcaligenes in oil reservoirs.
In the A1-4 panel, Bacillus (85.1%), Dietzia (9.5%) and Alcaligenes (3.6%) were detected, and hence the Acinetobacter bouvatii CGMCC NO.1.10395 activated the Bacillus, Dietzia and Alcaligenes in the reservoir.
in the experimental group a1-5, Pseudomonas genera (73.5%, and the added microbial species were not the same genus species), Bacillus genera (13.0%), Paenibacillus genera (7.1%), and Dietzia (5.2%) were detected, thus Pseudomonas kunmingensis CGMCC No.1.16086 activated Pseudomonas genera, Bacillus genera, Paenibacillus genera, and Dietzia genera in the oil reservoir.
In the experimental group a1-6, Pseudomonas (73.5%), Proteobacteria (17.9%), Bacillus (7.1%) and Dietzia (4.2%) were detected, thus Dietzia natronolimnaacgmcc No.1.6147 activated Pseudomonas, Proteobacteria, Bacillus and Dietzia in the reservoir.
As can be seen from the above, the acinetobacter of the exogenous bacteria in A1-1 and A1-4 can activate any one or any combination of Bacillus, Pseudomonas, Dietzia and Alcaligenes in the endogenous bacteria of the oil deposit;
the Dietzia serving as the exogenous bacteria in A1-2, A1-3 and A1-6 can activate any one or any combination of Pseudomonas, Bacillus, Proteobacteria, Dietzia, Paenibacillus, Microbacterium and Alcaligenes in the endogenous bacteria of the oil reservoir;
The exogenous bacterium Pseudomonas in A1-5 can activate any one or any combination of Pseudomonas, Bacillus, Paenibacillus and Dietzia in the oil deposit endogenous bacteria.
The results show that the exogenous bacterium acinetobacter can activate the bacillus, the pseudomonas, the dietzia and the like in the oil reservoir endogenous bacteria; the Dietzia serving as an exogenous bacterium can activate the bacillus, the pseudomonas and the like in the endogenous bacteria in the oil reservoir; the pseudomonas of the external bacteria can activate pseudomonas, Dietzia and Paenibacillus in the oil reservoir internal bacteria.
Different exogenous microorganisms can activate different endogenous microorganisms, so that the abundance of the endogenous microorganisms is improved, including the core microorganism Pseudomonas and other functional microorganisms which activate oil reservoirs, and the microorganisms cooperate with each other to accelerate the biodegradation of pollutants in the environment.
the above results show that:
"Bioaugmentation" is indistinguishable from "biostimulation," which is a form of "biostimulation. The 'bioaugmentation' exogenous microorganisms can directionally 'biostimulate' to activate endogenous microorganisms, and the pollution treatment efficiency is obviously improved.
Method for improving crude oil recovery ratio by injecting Dietzia to activate endogenous microorganism
The 2 high water content thin oil wells of a certain oil field in the eastern region are in a high water content state, and the reservoir information is shown in the following table 3.
TABLE 3
| Reservoir temperature C | 38 |
| Viscosity mPa.s at 30 DEG C | 8736 |
| Relative density of crude oil | 0.9733 |
| Freezing point deg.C | 25 |
| Contains wax% | 15.53 |
| has water content of% | 89 |
| Porosity (%) | 24 |
endogenous microorganisms in the crude oil of the reservoir include bacillus, pseudomonas, dietzia, and paenibacillus.
From the above results, it can be seen that the acinetobacter of the exogenous bacterium can activate the bacillus, pseudomonas and dietzia among the endogenous bacteria in the oil reservoir; the Dietzia serving as an exogenous bacterium can activate the bacillus, the pseudomonas and the like in the endogenous bacteria in the oil reservoir; the pseudomonas of the external bacteria can activate pseudomonas, Dietzia and Paenibacillus in the oil reservoir internal bacteria.
in order to verify the oil displacement effect of different exogenous bacteria, the following indoor physical model oil displacement experiment is carried out:
1. indoor physical model oil displacement experiment
(1) according to the oil reservoir permeability (24%), a high-pressure mould pipe (specification phi 25x300mm purchased from Haian Petroleum research instruments Co., Ltd.) is filled with quartz sand with different meshes to form a sand filling mould pipe. And measuring the permeability of the model pipe by adopting a permeability measuring device, and selecting the model pipe which meets the permeability. Vacuumizing saturated formation water, and calculating porosity; the parameters of the finally prepared model tubes for the subsequent steps are shown in table 4.
TABLE 4
The microbial strains listed in the above table are specifically as follows:
Acinetobacter baileyi berezianae CGMCC NO. 1.12683;
Dietzia serrata (CGMCC NO. 1.6332);
Dietzia alcalophila CGMCC NO. 1.6149;
Acinetobacter baumannii CGMCC NO. 1.10395;
Pseudomonas kunmingensis CGMCC NO. 1.16086;
Dietzia alchornea Dietzia natronolimnaea CGMCC NO. 1.6147.
(2) Continuously injecting crude oil from the mouth of the model pipe (the temperature is set to 55 ℃ because the consistency of the crude oil is higher) until the outlet of the model pipe detects the outflow of the crude oil, and measuring the original oil saturation; and aged at reservoir temperature (38 ℃) for 7 days.
3. And (4) displacing oil by using injected water, stopping water drive until the outlet produced liquid reaches the limit water content (98%), and calculating the recovery ratio (namely the water drive recovery ratio).
Water flooding recovery ratio is 100% of water flooding crude oil quantity/total crude oil quantity
4. injection volume as per volume of 1 model tube orifice (model tube volume (pi 25)2300/4) porosity by gasometry) was injected into the microbial injection solution and incubated at reservoir temperature (38 ℃) for 30 days.
The preparation method of the microorganism injection liquid comprises the following steps:
And (3) microbial liquid: inoculating the activated microorganism strains of each group (column 5 of Table 4) in LB culture medium, performing shaking culture at 30 deg.C and 150rpm for 24 hr to obtain microorganism solution of each group (with a concentration of 10 or more)8cfu/mL)；
the microorganism bacterium liquid in the experimental group G is obtained by mixing a bacterium liquid of the bacterium of the addict.
nutrient solution: 4g/L NH4NO3；4g/L KH2PO4；5.68g/L Na2HPO42g/L of peptone and 1g/L of yeast powder, and the balance of injected water;
Microorganism injection liquid: adding the above groups of microbial solutions into the nutrient solution to make the volume percentage of the microbial solution in the nutrient solution be 1% (after dilution, the thallus is propagated to make its thallus concentration be greater than or equal to 10)6cfu/mL)。
5. Then using injected water to displace oil, stopping water drive until the outlet produced liquid reaches the limit water content (98%), calculating the recovery ratio (namely the recovery ratio of microbial oil production)
Microbial recovery factor (microbial flooding crude oil amount/total crude oil amount) 100%
The results of the recovery factor measurements in the water flooding and exogenous microorganism injection processes are shown in Table 5.
TABLE 5 recovery ratio in water flooding and injection of exogenous microorganisms
| Experiment number | Recovery ratio (%) | Recovery ratio (%) |
| Water drive | 16.46 | / |
| Experimental group A | 25.55 | 9.09 |
| Experimental group B | 33.08 | 16.62 |
| Experimental group C | 35.67 | 19.21 |
| Experimental group D | 27.08 | 10.62 |
| Experimental group E | 29.47 | 13.01 |
| experimental group F | 37.23 | 20.77 |
| Experimental group G | 42.25 | 25.79 |
| Control group CK | 19.02 | 2.56 |
In the above table, the recovery ratio increasing formula in the third column is microbial oil recovery ratio-water flooding recovery ratio
Indoor physical model oil displacement shows that the oil displacement efficiency of the microorganism using the Dietzia is higher than that of other two microorganisms, and the oil displacement efficiency of the mixed flora constructed by the three Dietzia is higher than that of a single Dietzia, so that the recovery ratio can be improved by 25.79 percent.
2. Oilfield microbial huff and puff operation
Carrying out microorganism huff and puff operation on a certain oil well of the oil field, and adding exogenous microorganism Dietzia maris CGMCC NO.1.6332 bacterial liquid (the bacterial concentration is more than or equal to 10)8cfu/mL), Dietzia psychralcaliphila CGMCC NO.1.6149 bacterial liquid (bacterial concentration is more than or equal to 10)8cfu/mL) and Dietzia natronolimnaae CGMCC NO.1.6147 bacterial liquid (bacterial concentration is more than or equal to 10)8cfu/mL) preparing a mixed microbial inoculum according to the cfu ratio of 1:1:1, and then diluting the mixed microbial inoculum and the nutrient solution according to the volume ratio of 1:99 (the diluted bacteria are propagated to ensure that the concentration of the bacteria is more than or equal to 10)6cfu/mL) to obtain diluted bacterial liquid.
The amount of the diluted bacterial liquid is 500m3(the injection amount calculation method refers to the 2-month release P181-182 of the chemical industry publishing Co. 2009 of the petroleum and microbial oil recovery technology;) the single well is injected into the crude oil of the underground oil reservoir, and the well is stewed for 2 months before well opening production.
The above injection methods are specifically described in the following patents: the invention discloses a microbial oil recovery method by authorizing CN102213087B to combine swallowing and displacing.
The oil well production was measured 8 months after the injection of the microbes.
as a result, as shown in Table 6, the water content of the oil well decreased by 17.1% within 8 months after the injection of the exogenous microorganism, and the average daily oil production increased by about 2.5 tons.
TABLE 6 oil well production before and after exogenous microorganism injection
The results show that:
The Dietzia can directionally activate the indigenous oil-producing microorganism to obviously improve the crude oil recovery ratio.
Claims (10)
1. A method for improving the processing efficiency of a sample to be processed is to activate the activity of endogenous microorganisms in the sample to be processed by using Dietzia microorganisms so as to improve the processing efficiency of the sample to be processed;
the sample to be processed is crude oil, the water body polluted by the crude oil or the soil polluted by the crude oil;
The improvement of the treatment efficiency of the sample to be treated is the improvement of the recovery rate of crude oil or the improvement of the pollution treatment effect of the crude oil polluted water body or the crude oil polluted soil.
2. The method of claim 1, wherein:
The activity of using a Dietzia microorganism to activate an endogenous microorganism in a sample to be treated is to add the Dietzia microorganism to the sample to be treated.
3. The method according to claim 1 or 2, characterized in that:
The enhanced oil recovery is by emulsifying or facilitating flow of the crude oil;
Or the pollution treatment effect of the crude oil polluted water or the crude oil polluted soil is improved when the water quality index is improved or the content of organic pollutants is reduced.
4. a method according to any one of claims 1-3, characterized in that: the endogenous microorganisms in the sample to be treated include bacillus, pseudomonas and/or proteus.
5. Use of a microorganism of the Dietzia genus of any one of the claims 1-4 for enhanced oil recovery.
6. Use according to claim 5, characterized in that: the endogenous microorganisms in the crude oil include bacillus, pseudomonas, and/or proteus species.
7. Use according to claim 5 or 6, characterized in that: the enhanced oil recovery is achieved by emulsifying the oil or by facilitating the flow of the oil.
8. Use of a microorganism belonging to the genus Dietzia in the method according to any one of claims 1 to 4 for improving the effect of treating a water body contaminated with crude oil or improving the effect of treating a soil contaminated with crude oil.
9. Use according to claim 8, characterized in that:
the microorganisms in the crude oil-contaminated water body or the crude oil-contaminated soil include genus bacillus, genus pseudomonas and/or genus proteus.
10. Use according to claim 8 or 9, characterized in that:
The improvement of the pollution treatment effect of the crude oil polluted water or the crude oil polluted soil is reflected in that the water quality index becomes better or the content of organic pollutants is reduced.
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