CN106929558B - A kind of feeding astaxanthin wettable powder and preparation method thereof - Google Patents
A kind of feeding astaxanthin wettable powder and preparation method thereof Download PDFInfo
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- CN106929558B CN106929558B CN201710270374.9A CN201710270374A CN106929558B CN 106929558 B CN106929558 B CN 106929558B CN 201710270374 A CN201710270374 A CN 201710270374A CN 106929558 B CN106929558 B CN 106929558B
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- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
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Abstract
The invention discloses a kind of feeding astaxanthin wettable powders and preparation method thereof, belong to field of feed additive technology.The feeding astaxanthin wettable powder is by collecting the phaffiafhodozyma to ferment through liquid high-density, phaffiafhodozyma dry bacterium powder is obtained after concentrated, spray drying, astaxanthin ointment is isolated by broken wall, astaxanthin ointment is mixed with the edible soybean oil containing BHT, astaxanthin oil is made, astaxanthin oil is dispersed in beta cyclodextrin, finally embedded with prolan polysaccharide, in astaxanthin wettable powder obtained, content astaxanthin is 0.1 5%, and water content is 4 8%.
Description
Technical field
The present invention relates to field of feed additive technology, and more specifically more particularly to a kind of feeding astaxanthin is water-dispersed
Pulvis and preparation method thereof.
Background technology
Astaxanthin is a kind of carotenoid being widely present in organism.Animal itself cannot synthesize class recklessly in nature
Radish element, it is necessary to carotenoid is obtained from diet, carotenoid can improve color possessed by animal and animal's products, increase
The immunocompetence of strong animal.Astaxanthin is the terminal of carotenogenesis, it can be without modification or biochemistry after entering animal body
It converts and directly stores and be deposited in tissue, so that the skin of some fish is showed bright-coloured red, the orange, Huang Dengse of health, make fowl
Egg and the feather of fowl, skin, foot, beak show the golden yellow of health, red, improve the survival rate of aquatic fish.
For the production of natural astaxanthin, although certain green alga content astaxanthins are very high, the configuration of astaxanthin has
Difference, as the shell-fish byproduct such as shrimp shell, although abundant raw material, because its pigment content is low, extraction cost is higher, practical to use
Amount is restricted.And microbial reproduction is fast, growth cycle is short, and zymotechnique is more mature, furthermore phaffiafhodozyma itself is sought
Foster value is higher, is a kind of good food or feedstuff, therefore it is a pole to prepare astaxanthin with phaffiafhodozyma fermentation
Good developing direction.But phaffiafhodozyma cell change in wall is hard, and astaxanthin is unfavorable for animal use, and astaxanthin does not dissolve in
Water, while the aerobic stability of astaxanthin is poor, thus the existing forms of astaxanthin need to be changed, extend its bin stability and raising
Utilization rate.
Invention content
It is an object of the invention in view of the above shortcomings of the prior art, provide a kind of stabilization, can be uniformly dispersed in water
In astaxanthin product and preparation method thereof, the effect of giving full play to astaxanthin, extend the shelf-life of astaxanthin.
To achieve the above object, present invention employs following technical solutions：
A kind of preparation method of feeding astaxanthin wettable powder, includes the following steps：
(1) biosynthesis of astaxanthin：
1. the culture of phaffiafhodozyma seed accesses phaffiafhodozyma strain in Yeast Malt Extract culture medium, trained in seed
Seed culture is carried out under the conditions of supporting, and obtains phaffiafhodozyma seed liquor；
2. phaffiafhodozyma seed liquor is inoculated into fermentation medium by the fermented and cultured of phaffiafhodozyma, in fermentation condition
Lower carry out fermented and cultured obtains phaffiafhodozyma zymotic fluid；
(2) it concentrates：
After fermentation, phaffiafhodozyma zymotic fluid is concentrated, obtains phaffiafhodozyma Fermented Condensed liquid；
(3) it is spray-dried：
Suitable vitamin C, mixing are added into phaffiafhodozyma Fermented Condensed liquid, then by centrifugal spray drying, are obtained
Obtain phaffiafhodozyma dry bacterium powder；
(4) preparation of astaxanthin oil：
Broken wall treatment is carried out to phaffiafhodozyma dry bacterium powder, obtains astaxanthin ointment；A small amount of antioxidant BHT is taken to be dissolved in food
It is uniformly mixed therewith in soya-bean oil, then by astaxanthin ointment, astaxanthin oil is made；
(5) preparation of astaxanthin wettable powder：
Astaxanthin oil is scattered in beta-cyclodextrin, prolan polysaccharide is added, astaxanthin water is made in fully dispersed mixing
Dispersion powder.
Preferably, in the biosynthesis of step (1) astaxanthin, the culture of phaffiafhodozyma seed is by red hair husband
Yeast starter is inoculated in Yeast Malt Extract culture medium, i.e. glucose 10g, soy peptone 5g, yeast extract 3g, fructus hordei germinatus leaching powder
3g, pure water 1000ml, pH 5.5-6.2,115 DEG C of high pressure sterilization 15min, 20-22 DEG C of cultivation temperature, incubation time 48-52h are obtained
Obtain phaffiafhodozyma seed liquor.
Preferably, in the biosynthesis of step (1) astaxanthin, the fermented and cultured of phaffiafhodozyma is by red hair husband
Yeast starter liquid is inoculated into fermentation medium, i.e. sucrose 36kg, yeast extract 4.2kg, potassium dihydrogen phosphate 0.18kg, ammonium sulfate
7.2kg, tap water 600L, pH 5.5-6.2,121 DEG C of high pressure sterilization 30min；Cooling is followed by kind of a seed liquor, cultivation temperature 20-22
DEG C, incubation time 48-72h obtains phaffiafhodozyma zymotic fluid.
Preferably, in the step (3) ascorbic additive amount be phaffiafhodozyma Fermented Condensed liquid weight 0.1-
1.0%；The condition of centrifugal spray drying is：Control 150-160 DEG C of dryer entrance temperature, 60-70 DEG C of leaving air temp.
Preferably, in the step (4) BHT additive amount be astaxanthin ointment weight 0.01-2%；Edible soybean oil
Dosage is 1-2 times of astaxanthin ointment weight.
Preferably, the dosage of beta-cyclodextrin is 5-10 times of astaxanthin ointment weight in the step (5)；Prolan polysaccharide
Dosage be astaxanthin ointment weight 5-10%.
Using feeding astaxanthin wettable powder, content astaxanthin 0.1-5%, water content made of the method for the present invention
For 4-8%, storage can be stablized under normal temperature condition, when use can be uniformly dispersed in water.
Figure of description
Fig. 1 is the curve graph that the feeding astaxanthin wettable powder of 0.6% content is stored at room temperature.
Specific implementation mode
For a better understanding of the present invention, with reference to the embodiment content that the present invention is furture elucidated, but the present invention
Content is not limited solely to the following examples.
Embodiment 1
The preparation of the feeding astaxanthin wettable powder of 0.6% content：
(1) biosynthesis of astaxanthin
1. the culture of phaffiafhodozyma seed
Phaffiafhodozyma strain is inoculated in Yeast Malt Extract culture medium, i.e. glucose 10g, soy peptone 5g, yeast
Soak 6.0,115 DEG C of powder 3g, fructus hordei germinatus leaching powder 3g, pure water 1000ml, pH high pressure sterilization 15min, 20 DEG C -22 DEG C of cultivation temperature, culture
Time 50h obtains phaffiafhodozyma seed liquor；
2. the fermented and cultured of phaffiafhodozyma
Phaffiafhodozyma seed liquor is inoculated into fermentation medium, i.e. sucrose 36kg, yeast extract 4.2kg, potassium dihydrogen phosphate
6.0,121 DEG C of 0.18kg, ammonium sulfate 7.2kg, tap water 600L, pH high pressure sterilization 30min；Cooling is followed by kind of a seed liquor, cultivates
20 DEG C -22 DEG C, incubation time 69h of temperature obtains phaffiafhodozyma zymotic fluid；
(2) it concentrates
After fermentation, phaffiafhodozyma zymotic fluid is concentrated using centrifuge, collects Fermented Condensed liquid 81L；
(3) it is spray-dried
Vitamin C 0.81Kg is added into phaffiafhodozyma Fermented Condensed liquid, mixing passes through LPG-5 type centrifugal spray dryers
Dry machine drying, controls 160 DEG C of inlet air temperature, and 70 DEG C of leaving air temp collects phaffiafhodozyma dry bacterium powder 17.5Kg；
(4) preparation of astaxanthin oil
Broken wall treatment is carried out to phaffiafhodozyma dry bacterium powder by the squeezing of 6YL-80 type squeezers, isolates astaxanthin ointment
1.6kg；Edible soybean oil 1.6kg, BHT 32g is weighed, BHT is dissolved in edible soybean oil, then astaxanthin ointment is uniform therewith
Mixing, obtains astaxanthin oil 3.2kg；
(5) preparation of astaxanthin wettable powder
Beta-cyclodextrin 8.2kg is added in FL1.5 type high speed dispersors, starts dispersion machine, is slowly added to astaxanthin oil
3.2kg disperses 5 minutes, adds Prolan 110g, fully dispersed 12 minutes, collects astaxanthin wettable powder product
10.8kg, content astaxanthin 0.6%, water content 5.4%.
Embodiment 2
The preparation of the feeding astaxanthin wettable powder of 0.62% content：
(1) biosynthesis of astaxanthin
1. the culture of phaffiafhodozyma seed
Phaffiafhodozyma strain is inoculated in Yeast Malt Extract culture medium, i.e. glucose 10g, soy peptone 5g, yeast
Soak 1000ml, pH5.7,115 DEG C of powder 3g, fructus hordei germinatus leaching powder 3g, pure water high pressure sterilization 15min, 20 DEG C -22 DEG C of cultivation temperature, culture
Time 48h obtains phaffiafhodozyma seed liquor；
2. the fermented and cultured of phaffiafhodozyma
Phaffiafhodozyma seed liquor is inoculated into fermentation medium, i.e. sucrose 36kg, yeast extract 4.2kg, potassium dihydrogen phosphate
600L, pH5.7,121 DEG C of 0.18kg, ammonium sulfate 7.2kg, tap water high pressure sterilization 30min；Cooling is followed by kind of a seed liquor, cultivates
20 DEG C -22 DEG C, incubation time 65h of temperature obtains phaffiafhodozyma zymotic fluid；
(2) it concentrates
After fermentation, phaffiafhodozyma zymotic fluid is concentrated using centrifuge, collects Fermented Condensed liquid 79L；
(3) it is spray-dried
Vitamin C 395g is added into phaffiafhodozyma Fermented Condensed liquid, mixing passes through LPG-5 type centrifugal spray dryings
Machine is dried, and 150 DEG C of inlet air temperature is controlled, and 60 DEG C of leaving air temp collects phaffiafhodozyma dry bacterium powder 18.6Kg；
(4) preparation of astaxanthin oil
Broken wall treatment is carried out to phaffiafhodozyma dry bacterium powder by the squeezing of 6YL-80 type squeezers, isolates astaxanthin ointment
1.54kg；Edible soybean oil 1.54kg, BHT 30.8g is weighed, BHT is dissolved in edible soybean oil, then therewith by astaxanthin ointment
Uniformly mixing, obtains astaxanthin oil 3.08kg；
(5) preparation of astaxanthin wettable powder
Beta-cyclodextrin 8.1kg is added in FL1.5 type high speed dispersors, starts dispersion machine, is slowly added to astaxanthin oil
3.08kg disperses 5 minutes, adds prolan polysaccharide 110g, fully dispersed 12 minutes, collects astaxanthin wettable powder product
10.4kg, content astaxanthin 0.62%, water content 5.4%.
Claims (7)
1. a kind of preparation method of feeding astaxanthin wettable powder, it is characterised in that：Include the following steps：
(1) biosynthesis of astaxanthin：
1. the culture of phaffiafhodozyma seed accesses phaffiafhodozyma strain in Yeast Malt Extract culture medium, trained in seed
Seed culture is carried out under the conditions of supporting, and obtains phaffiafhodozyma seed liquor；
In the biosynthesis of step (1) astaxanthin, the culture of phaffiafhodozyma seed is to be inoculated with phaffiafhodozyma strain
In Yeast Malt Extract culture medium, i.e. glucose 10g, soy peptone 5g, yeast extract 3g, fructus hordei germinatus leaching powder 3g, pure water 1000ml,
PH 5.5-6.2,115 DEG C of high pressure sterilization 15min, 20-22 DEG C of cultivation temperature, incubation time 48-52h obtain phaffiafhodozyma kind
Sub- liquid；
2. phaffiafhodozyma seed liquor is inoculated into fermentation medium by the fermented and cultured of phaffiafhodozyma, in fermentation condition
Lower carry out fermented and cultured obtains phaffiafhodozyma zymotic fluid；
(2) it concentrates：After fermentation, phaffiafhodozyma zymotic fluid is concentrated, obtains phaffiafhodozyma Fermented Condensed
Liquid；
(3) it is spray-dried：
Suitable vitamin C, mixing are added into phaffiafhodozyma Fermented Condensed liquid, then by centrifugal spray drying, are obtained red
Send out husband's yeast dry bacterium powder；
(4) preparation of astaxanthin oil：
Broken wall treatment is carried out to phaffiafhodozyma dry bacterium powder, obtains astaxanthin ointment；
It takes a small amount of antioxidant BHT to be dissolved in edible soybean oil, then astaxanthin ointment is uniformly mixed therewith, astaxanthin oil is made；
(5) preparation of astaxanthin wettable powder：
Astaxanthin oil is scattered in beta-cyclodextrin, prolan polysaccharide is added, it is water-dispersed that astaxanthin is made in fully dispersed mixing
Pulvis.
2. the preparation method of feeding astaxanthin wettable powder according to claim 1, it is characterised in that：The step
(1) in the biosynthesis of astaxanthin, the fermented and cultured of phaffiafhodozyma is that phaffiafhodozyma seed liquor is inoculated into fermented and cultured
Base, i.e. sucrose 36kg, 4.2 kg of yeast extract, potassium dihydrogen phosphate 0.18kg, ammonium sulfate 7.2kg, tap water 600L, pH 5.5-
6.2,121 DEG C of high pressure sterilization 30min；Cooling is followed by kind of a seed liquor, 20-22 DEG C of cultivation temperature, and incubation time 48-72h is obtained red
Send out husband's yeast fermentation broth.
3. the preparation method of feeding astaxanthin wettable powder according to claim 1, it is characterised in that：The step
(3) ascorbic additive amount is the 0.1-1.0% of phaffiafhodozyma Fermented Condensed liquid weight in.
4. the preparation method of feeding astaxanthin wettable powder according to claim 1, it is characterised in that：The step
(3) condition of centrifugal spray drying is in：Control 150-160 DEG C of dryer entrance temperature, 60-70 DEG C of leaving air temp.
5. the preparation method of feeding astaxanthin wettable powder according to claim 1, it is characterised in that：The step
(4) additive amount of BHT is the 0.01-2% of astaxanthin ointment weight in；The dosage of edible soybean oil is the 1-2 of astaxanthin ointment weight
Times.
6. the preparation method of feeding astaxanthin wettable powder according to claim 1, it is characterised in that：The step
(5) dosage of beta-cyclodextrin is 5-10 times of astaxanthin ointment weight in；The dosage of prolan polysaccharide is astaxanthin ointment weight
5-10%.
7. a kind of feeding astaxanthin wettable powder, it is characterised in that：Using the preparation of any one of claim 1-6 the methods
At content astaxanthin 0.1-5%, water content 4-8%.
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