CN106924456A - One kind is for activating mitochondrial composition - Google Patents
One kind is for activating mitochondrial composition Download PDFInfo
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Abstract
The invention discloses one kind for activating mitochondrial composition, it is prepared from by the raw material of following weight portion：10 20 parts of coumestrol, 5 10 parts of Co-Q10,10 20 parts of ellagic acid, 13 parts of Quercetin, 5 10 parts of grape seed extract, 10 15 parts of extract of Milk Thistle grass, 10 15 parts of Olive leaf P.E, 10 15 parts of Common Borage, 36 parts of nostoc yeast powder, 48 parts of ganoderma spove powder, 10 15 parts of acetylcarnitine, 5 10 parts of hickory nut bark ultramicro grinding powder.Resulting composition of the present invention can improve the activity of cyclophorase and increase the quantity of mitochondrial DNA, so the composition can activate mitochondria and increase mitochondrial biosynthesis.Therefore, the present composition can be effectively used for preventing or treating the various diseases relevant with mitochondria activity reduction, such as degenerative disorders, parkinsonism.
Description
Technical field
Present invention relates particularly to one kind for activating mitochondrial composition.
Background technology
Mitochondria is a kind of organelle that can be found in most eukaryotics.Mitochondria has the DNA of oneself, i.e.,
Independently of the DNA (mtDNA) outside nucleus DNA.
The most important effect of mitochondria is generation atriphos (ATP), and ATP is intracellular energy source.With line grain
Body Medium Culture produce NADH and FADH2, by TCA circulate (tricarboxylic acid cycle), from electron transport chain produce ATP.Therefore, produce
Raw ATP is for activating the various biosynthesis for needing energy and metabolic process.
Mitochondria can also briefly store calcium ion in matrix, and calcium ion is extremely important for intracellular signal transduction,
And calcium ion can be discharged into cytoplasm when needed.Furthermore it is known that mitochondria is bred and thin in Apoptosis, cell
Central regulating and controlling effect is played in the activities such as born of the same parents' metabolism.
Mitochondrial DNA is relative to be vulnerable to damage, because it is different from nucleus DNA, lacks own healing mechanism, and do not have
There is the histone of protection DNA.It is well known that the damage of mitochondrial DNA is closely related with the morbidity of mitochondrial disease.Mitochondria work(
The degeneration of energy, can cause the reduction of energy source ATP synthesis needed for cellular activity, so as to give rise to diseases.
The content of the invention
To solve the above problems, the invention provides one kind for activating mitochondrial composition.
To achieve the above object, the technical scheme taken of the present invention is：
One kind is prepared from for activating mitochondrial composition by the raw material of following weight portion：
Coumestrol 10-20 parts, Co-Q10 5-10 parts, ellagic acid 10-20 parts, Quercetin 1-3 parts, grape seed extract
5-10 parts, Milk Thistle grass 10-15 parts of extract, Olive leaf P.E 10-15 parts, Common Borage 10-15 parts, nostoc yeast powder 3-6
Part, ganoderma spove powder 4-8 parts, acetylcarnitine 10-15 parts, 5-10 parts of hickory nut bark ultramicro grinding powder
Wherein, the grape seed extract is as obtained by prepared by following steps：
S1, by grape pip saline sook 0.5~1 hour after, be placed in flash extracter treatment in, after flash treatment, add 3
~6 times of water, by mass percentage for 0.5~1.5% ratio add neutral proteinase, at 30~40 DEG C digest 0.5~2h, mistake
Filter, obtains enzymolysis liquid；
S2, by enzymolysis liquid directly by neutral alumina column, resin column blade diameter length ratio is 1: 6~1: 11, respectively with 1~3 times
Cylinder ponding, the organic solution of 1~2 times of column volume 95% elute removal of impurities, with 6~10 times of 0.3~0.7% glacial acetic acid of column volume
~50% organic solvent (1: 1) eluant solution, collects eluent；
S3, by eluent concentrate drying, obtain grape seed extract.
Wherein, the Milk Thistle grass extract is as obtained by prepared by following steps：
Dry Milk Thistle grass is processed with flash extracter, gained extract solution is first heated with high heat, is added with slow fire after boiling
Hot 1-1.5h, filtering and concentrating is slowly added to 90-100% ethanol to 1.5g/ml, until concentration of alcohol is 55-65%, refrigerates 8-9
Hour, supernatant is removed after 2000-4000r/min centrifugations 15-25min, precipitation deionized water is dissolved to original volume, uses ethanol
Precipitation 2 times, concentration of alcohol is respectively 65-75%, 75-85%；Precipitation deionized water is dissolved to original volume, adds 1/6-1/5
The solution of trichloroacetic acid of volume 10-12%, stands 4-6h after fully mixing, go to sink after 2000-4000r/min centrifugations 15-25min
Form sediment to obtain supernatant, and by gained supernatant suction filtration, drying obtains Milk Thistle grass extract.
Wherein, the Olive leaf P.E is as obtained by prepared by following steps：
Freeze-drying after olive leaf cleaning is taken, is smashed, room temperature is concentrated into relative density 1.10-1.20 after defrosting, spraying is dry
After dry concentrate, CO is carried out2Supercritical extract, fluid extraction pressure is 30-50MPa, 30-40 DEG C of extraction temperature, CO2Fluid stream
It is 750-850L/h to measure, and extraction time is 3-5h, obtains Olive leaf P.E.
Wherein, the nostoc yeast powder is as obtained by prepared by following steps：
Take the nostoc cleaned after draining to be placed in steam-explosion jar, it is 0.7- to be first passed through nitrogen to steam explosion pressure inside the tank
1.5MPa, explosion treatment 7-23min；Then it is 1.5-2.1MPa to be passed through steam to steam explosion pressure inside the tank rapidly, at steam blasting
After reason 0.8-2.8min, according to the ratio microbe inoculation leavening of inoculum concentration 1.5-1.7%, 25-35 DEG C of keeping temperature, fermentation
After 48-54 hours, high-temperature sterilization obtains nostoc yeast powder.
Wherein, the microbe leaven is by Bacillus acidi lactici, bacillus licheniformis, Corynebacterium glutamicum in mass ratio 3: 2:
1 mixing gained.
Wherein, the ganoderma spove powder is as obtained by prepared by following steps：
S1, the Reishi sporule that clean will be drained are placed in steam-explosion jar, and it is 0.6- to be first passed through nitrogen to steam explosion pressure inside the tank
1.4MPa, explosion treatment 8-25min；Then it is 1.4-1.8MPa to be passed through steam to steam explosion pressure inside the tank rapidly, at steam blasting
After reason 0.5-2.5min, room temperature is concentrated into relative density 1.10-1.20, is spray-dried concentrate, obtains powder；
S2, in powder add 3-8 times of water, by mass percentage be 2-3% ratio add neutral proteinase, by quality
Percentage is that 1-2% adds flavor protease, and 1-2h is digested at 30-50 DEG C, is filtered, and obtains enzymolysis liquid；
S3, by naturally to thaw again after the zymotic fluid quick freeze of gained, age of starch is precipitated in making extract；Collect supernatant
Liquid, remainder centrifugation is collected centrifugal liquid and is incorporated in supernatant；
S4, by supernatant with the flow velocity of 3-7BV/h by macroporous resin column, after Dynamic Adsorption saturation, with deionized water with
The above-mentioned macroporous resin column of flow velocity drip washing of 5-8BV/h is colourless to efflux, then with the organic solvent that volume fraction is 50%-70%
Eluted with the flow velocity of 8-12BV/h, collected eluent, freeze-drying is obtained ganoderma spove powder.
The invention has the advantages that：
Resulting composition can improve the activity of cyclophorase and increase the quantity of mitochondrial DNA, so the composition energy
Activation mitochondria simultaneously increases mitochondrial biosynthesis.Therefore, the present composition can be effectively used for preventing or treating each
Plant the disease relevant with mitochondria activity reduction, such as degenerative disorders, parkinsonism.
Specific embodiment
In order that objects and advantages of the present invention become more apparent, the present invention is carried out further with reference to embodiments
Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
In following examples, the grape seed extract for being used is as obtained by prepared by following steps：
S1, by grape pip saline sook 0.5~1 hour after, be placed in flash extracter treatment in, after flash treatment, add 3
~6 times of water, by mass percentage for 0.5~1.5% ratio add neutral proteinase, at 30~40 DEG C digest 0.5~2h, mistake
Filter, obtains enzymolysis liquid；
S2, by enzymolysis liquid directly by neutral alumina column, resin column blade diameter length ratio is 1: 6~1: 11, respectively with 1~3 times
Cylinder ponding, the organic solution of 1~2 times of column volume 95% elute removal of impurities, with 6~10 times of 0.3~0.7% glacial acetic acid of column volume
~50% organic solvent (1: 1) eluant solution, collects eluent；
S3, by eluent concentrate drying, obtain grape seed extract.
Wherein, the Milk Thistle grass extract is as obtained by prepared by following steps：
Dry Milk Thistle grass is processed with flash extracter, gained extract solution is first heated with high heat, is added with slow fire after boiling
Hot 1-1.5h, filtering and concentrating is slowly added to 90-100% ethanol to 1.5g/ml, until concentration of alcohol is 55-65%, refrigerates 8-9
Hour, supernatant is removed after 2000-4000r/min centrifugations 15-25min, precipitation deionized water is dissolved to original volume, uses ethanol
Precipitation 2 times, concentration of alcohol is respectively 65-75%, 75-85%；Precipitation deionized water is dissolved to original volume, adds 1/6-1/5
The solution of trichloroacetic acid of volume 10-12%, stands 4-6h after fully mixing, go to sink after 2000-4000r/min centrifugations 15-25min
Form sediment to obtain supernatant, and by gained supernatant suction filtration, drying obtains Milk Thistle grass extract.
Wherein, the Olive leaf P.E is as obtained by prepared by following steps：
Freeze-drying after olive leaf cleaning is taken, is smashed, room temperature is concentrated into relative density 1.10-1.20 after defrosting, spraying is dry
After dry concentrate, CO is carried out2Supercritical extract, fluid extraction pressure is 30-50MPa, 30-40 DEG C of extraction temperature, CO2Fluid stream
It is 750-850L/h to measure, and extraction time is 3-5h, obtains Olive leaf P.E.
Wherein, the nostoc yeast powder is as obtained by prepared by following steps：
Take the nostoc cleaned after draining to be placed in steam-explosion jar, it is 0.7- to be first passed through nitrogen to steam explosion pressure inside the tank
1.5MPa, explosion treatment 7-23min；Then it is 1.5-2.1MPa to be passed through steam to steam explosion pressure inside the tank rapidly, at steam blasting
After reason 0.8-2.8min, according to the ratio microbe inoculation leavening of inoculum concentration 1.5-1.7%, 25-35 DEG C of keeping temperature, fermentation
After 48-54 hours, high-temperature sterilization obtains nostoc yeast powder.
Wherein, the microbe leaven is by Bacillus acidi lactici, bacillus licheniformis, Corynebacterium glutamicum in mass ratio 3: 2:
1 mixing gained.
Wherein, the ganoderma spove powder is as obtained by prepared by following steps：
S1, the Reishi sporule that clean will be drained are placed in steam-explosion jar, and it is 0.6- to be first passed through nitrogen to steam explosion pressure inside the tank
1.4MPa, explosion treatment 8-25min；Then it is 1.4-1.8MPa to be passed through steam to steam explosion pressure inside the tank rapidly, at steam blasting
After reason 0.5-2.5min, room temperature is concentrated into relative density 1.10-1.20, is spray-dried concentrate, obtains powder；
S2, in powder add 3-8 times of water, by mass percentage be 2-3% ratio add neutral proteinase, by quality
Percentage is that 1-2% adds flavor protease, and 1-2h is digested at 30-50 DEG C, is filtered, and obtains enzymolysis liquid；
S3, by naturally to thaw again after the zymotic fluid quick freeze of gained, age of starch is precipitated in making extract；Collect supernatant
Liquid, remainder centrifugation is collected centrifugal liquid and is incorporated in supernatant；
S4, by supernatant with the flow velocity of 3-7BV/h by macroporous resin column, after Dynamic Adsorption saturation, with deionized water with
The above-mentioned macroporous resin column of flow velocity drip washing of 5-8BV/h is colourless to efflux, then with the organic solvent that volume fraction is 50%-70%
Eluted with the flow velocity of 8-12BV/h, collected eluent, freeze-drying is obtained ganoderma spove powder.
Embodiment 1
One kind is prepared from for activating mitochondrial composition by the raw material of following weight portion：
10 parts of coumestrol, 5 parts of Co-Q10,10 parts of ellagic acid, 1 part of Quercetin, 5 parts of grape seed extract, Milk Thistle grass
10 parts of extract, 10 parts of Olive leaf P.E, 10 parts of Common Borage, 3 parts of nostoc yeast powder, 4 parts of ganoderma spove powder, acetyl
10 parts of carnitine, 5 parts of hickory nut bark ultramicro grinding powder.
Embodiment 2
One kind is prepared from for activating mitochondrial composition by the raw material of following weight portion：
20 parts of coumestrol, 10 parts of Co-Q10,20 parts of ellagic acid, 3 parts of Quercetin, 10 parts of grape seed extract, Milk Thistle
15 parts of careless extract, 15 parts of Olive leaf P.E, 15 parts of Common Borage, 6 parts of nostoc yeast powder, 8 parts of ganoderma spove powder, second
15 parts of acylcarnitine, 10 parts of hickory nut bark ultramicro grinding powder.
Embodiment 3
One kind is prepared from for activating mitochondrial composition by the raw material of following weight portion：
15 parts of coumestrol, 7.5 parts of Co-Q10,15 parts of ellagic acid, 2 parts of Quercetin, 7.5 parts of grape seed extract, milk
12.5 parts of extract of Ji grass, 12.5 parts of Olive leaf P.E, 12.5 parts of Common Borage, 4.5 parts of nostoc yeast powder, wall-breaking lucidum spore
6 parts of sub- powder, 12.5 parts of acetylcarnitine, 7.5 parts of hickory nut bark ultramicro grinding powder.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
1. it is a kind of for activating mitochondrial composition, it is characterised in that to be prepared from by the raw material of following weight portion：
Coumestrol 10-20 parts, Co-Q10 5-10 parts, ellagic acid 10-20 parts, Quercetin 1-3 parts, grape seed extract 5-10
Part, Milk Thistle grass 10-15 part of extract, Olive leaf P.E 10-15 parts, Common Borage 10-15 parts, 3-6 parts of nostoc yeast powder, break
4-8 parts of wall lucidum spore powder, acetylcarnitine 10-15 parts, 5-10 parts of hickory nut bark ultramicro grinding powder.
2. it is as claimed in claim 1 a kind of for activating mitochondrial composition, it is characterised in that the grape seed extract
As obtained by prepared by following steps：
S1, by grape pip saline sook 0.5~1 hour after, be placed in flash extracter treatment in, after flash treatment, add 3~6
Times water, by mass percentage the ratio for 0.5~1.5% add neutral proteinase, digest 0.5~2h at 30~40 DEG C, filtering,
Obtain enzymolysis liquid；
S2, by enzymolysis liquid directly by neutral alumina column, resin column blade diameter length ratio is 1: 6~1: 11, respectively with 1~3 times of cylinder
The organic solution wash-out removal of impurities of ponding, 1~2 times of column volume 95%, with 6~10 times of 0.3~0.7% glacial acetic acid of column volume~
50% organic solvent (1: 1) eluant solution, collects eluent；
S3, by eluent concentrate drying, obtain grape seed extract.
3. it is as claimed in claim 1 a kind of for activating mitochondrial composition, it is characterised in that the Milk Thistle grass extract
As obtained by prepared by following steps：
Dry Milk Thistle grass is processed with flash extracter, gained extract solution is first heated with high heat, 1- is heated with slow fire after boiling
1.5h, filtering and concentrating is slowly added to 90-100% ethanol to 1.5g/ml, until concentration of alcohol is 55-65%, 8-9 is small for refrigeration
When, supernatant is removed after 2000-4000r/min centrifugations 15-25min, precipitation deionized water is dissolved to original volume, heavy with ethanol
Form sediment 2 times, concentration of alcohol is respectively 65-75%, 75-85%；Precipitation deionized water is dissolved to original volume, adds 1/6-1/5 bodies
The solution of trichloroacetic acid of product 10-12%, stands 4-6h after fully mixing, precipitation is gone after 2000-4000r/min centrifugations 15-25min
Supernatant is obtained, by gained supernatant suction filtration, drying obtains Milk Thistle grass extract.
4. it is as claimed in claim 1 a kind of for activating mitochondrial composition, it is characterised in that the Olive leaf P.E
As obtained by prepared by following steps：
Freeze-drying after olive leaf cleaning is taken, is smashed, room temperature is concentrated into relative density 1.10-1.20 after defrosting, be spray-dried dense
After contracting liquid, CO is carried out2Supercritical extract, fluid extraction pressure is 30-50MPa, 30-40 DEG C of extraction temperature, CO2Fluid flow is
750-850L/h, extraction time is 3-5h, obtains Olive leaf P.E.
5. it is as claimed in claim 1 a kind of for activating mitochondrial composition, it is characterised in that the nostoc yeast powder
As obtained by prepared by following steps：
Take the nostoc cleaned after draining to be placed in steam-explosion jar, it is 0.7-1.5MPa to be first passed through nitrogen to steam explosion pressure inside the tank, quick-fried
Tear manages 7-23min；Then it is 1.5-2.1MPa, Steam explosion treatment 0.8- to be passed through steam to steam explosion pressure inside the tank rapidly
After 2.8min, according to the ratio microbe inoculation leavening of inoculum concentration 1.5-1.7%, 25-35 DEG C of keeping temperature, ferment 48-54
After hour, high-temperature sterilization obtains nostoc yeast powder.
6. it is as claimed in claim 5 a kind of for activating mitochondrial composition, it is characterised in that the microbe leaven
Gained is mixed by Bacillus acidi lactici, bacillus licheniformis, Corynebacterium glutamicum in mass ratio 3: 2: 1.
7. it is as claimed in claim 1 a kind of for activating mitochondrial composition, it is characterised in that the wall-broken ganoderma spore
Powder is as obtained by prepared by following steps：
S1, the Reishi sporule that clean will be drained are placed in steam-explosion jar, and it is 0.6-1.4MPa to be first passed through nitrogen to steam explosion pressure inside the tank,
Explosion treatment 8-25min；Then it is 1.4-1.8MPa, Steam explosion treatment 0.5- to be passed through steam to steam explosion pressure inside the tank rapidly
After 2.5min, room temperature is concentrated into relative density 1.10-1.20, is spray-dried concentrate, obtains powder；
S2, in powder add 3-8 times of water, by mass percentage be 2-3% ratio add neutral proteinase, by quality percentage
Than adding flavor protease for 1-2%, 1-2h is digested at 30-50 DEG C, filtered, obtain enzymolysis liquid；
S3, by naturally to thaw again after the zymotic fluid quick freeze of gained, age of starch is precipitated in making extract；Collect supernatant,
Remainder is centrifuged, and collects centrifugal liquid and is incorporated in supernatant；
S4, by supernatant with the flow velocity of 3-7BV/h by macroporous resin column, after Dynamic Adsorption saturation, with deionized water with 5-
The above-mentioned macroporous resin column of flow velocity drip washing of 8BV/h is colourless to efflux, then with the organic solvent that volume fraction is 50%-70% with
The flow velocity of 8-12BV/h is eluted, and collects eluent, and freeze-drying obtains ganoderma spove powder.
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