CN106922954A - A kind of method for preparing chicken feet hide collagen raw material - Google Patents
A kind of method for preparing chicken feet hide collagen raw material Download PDFInfo
- Publication number
- CN106922954A CN106922954A CN201511028517.2A CN201511028517A CN106922954A CN 106922954 A CN106922954 A CN 106922954A CN 201511028517 A CN201511028517 A CN 201511028517A CN 106922954 A CN106922954 A CN 106922954A
- Authority
- CN
- China
- Prior art keywords
- chicken feet
- raw material
- hide collagen
- enzymolysis liquid
- collagen raw
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The present invention relates to a kind of method for preparing chicken feet hide collagen raw material, comprise the following steps：S100, chicken feet skin is mixed with water, add lipase, keratinase, anhydrous sodium sulfite, 2~4h is digested at pH8~10,40~50 DEG C, obtain the first enzymolysis liquid；S200, regulation the first enzymolysis liquid pH to 7.0~9.0, add HuPro protease, and 4~6h is digested at 50~70 DEG C, obtain the second enzymolysis liquid；S300, filtering second enzymolysis liquid, filtration cakes torrefaction are crushed, and obtain final product chicken feet hide collagen raw material.The present invention is by digesting the sturdy existing native protein resource rational utilization of chicken feet, and enzymolysis process is environment-friendly pollution-free, and obtained protein feed raw material is applied to be substituted during animal and fowl fodder is formulated or part substitutes protein raw materials.
Description
Technical field
The present invention relates to animal feed processing technique field, in particular, it is related to one kind to utilize enzyme preparation
Method of the degraded chicken feet skin to prepare chicken feet hide collagen raw material.
Background technology
The epidermis of chicken feet skin is different from the epidermis of skin of mammal, and wherein chicken feet crust is in chicken feet skin
Layer, there is thicker cuticula, is in chicken feet processing, the epidermis that can be backed off to be scalded through hot water.Chicken feet crust
The proportion for accounting for every broiler chicken weight is very low, but, with Chicken industry, chicken claw industry continue to develop and
The extensive use of chicken feet peeling machine, the production of chicken claw and processing quantity constantly rise, and obtain in the world every year
Chicken feet crust about reaches 100,000 tons or so.Long-time abandoned piles cause significant damage to ecological environment,
Health to human body constitutes significant threat, while being also that one kind of potential food resource is wasted.
In the last few years, the recycling for chicken feet crust gradually spreads out, and Cao Xiangfen etc. is first to chicken feet skin
Institutional framework studied and the key problem in technology that chicken feet leather makes high-grade decoration leather be described so that
Application of the chicken feet skin in terms of process hides is more ripe.Many animal feed companies pass through simple process by chicken feet
Crust is processed into feed applications in livestock and poultry cultivation, but utilization rate is relatively low.Both at home and abroad on chicken feet crust depth
Spend the report develop and useedd seldom, do not obtain association area scientist should have attention, correlative study
It is considerably less thus very limited to the understanding of chicken feet crust at present.
The content of the invention
The technical problems to be solved by the invention are：A kind of method for preparing chicken feet hide collagen raw material is provided,
Utilization rate is low when solving the problems, such as that chicken feet skin is added as feed in the prior art.
The present invention solve above-mentioned technical problem scheme be：A kind of chicken feet hide collagen raw material of preparing is provided
Method, comprises the following steps：
S100, chicken feet skin is mixed with water, add lipase, keratinase, anhydrous sodium sulfite,
PH8~10,2~4h is digested at 40~50 DEG C, obtain the first enzymolysis liquid；
S200, the first enzymolysis liquid pH to 7.0~9.0 is adjusted, add HuPro protease, 50~
4~6h is digested at 70 DEG C, the second enzymolysis liquid is obtained；
S300, filtering second enzymolysis liquid, filtration cakes torrefaction are crushed, and obtain final product chicken feet hide collagen raw material.
In the method for preparing chicken feet hide collagen raw material that the present invention is provided, in the step S100, chicken feet
Skin is 1: 8~1: 12 with the weight ratio of water, and the enzyme activity of lipase is 15~25U/mL, angle in the aqueous solution
The enzyme activity of protease is 7.5~15U/mL, and the mass fraction of aqueous solution sulfite sodium is 10~20g/L；
In enzymolysis process, shaking table shakes or stirs.
It is water-soluble in the step S200 in the method for preparing chicken feet hide collagen raw material that the present invention is provided
The enzyme activity of HuPro protease is 9.0~18.0U/mL in liquid；In enzymolysis process, shaking table shakes or stirs.
In the method for preparing chicken feet hide collagen raw material that the present invention is provided, in the step S300, filtering
Second enzymolysis liquid, contains asparatate, glutamic acid, serine, histidine, sweet ammonia in filtrate
Acid, threonine, arginine, alanine, tyrosine, cystine, valine, methionine, phenylpropyl alcohol ammonia
The content of acid, isoleucine, leucine, lysine, wherein essential amino acid is more than 50%.
In the method for preparing chicken feet hide collagen raw material that the present invention is provided, also wrapped before the step S100
Include：
S001, discarded chicken feet skin is collected from market, clean up, drain, directly used after oven drying
Or crush standby.
In the method for preparing chicken feet hide collagen raw material that the present invention is provided, in the step S300, filtering
Second enzymolysis liquid, filtrate dry powder is obtained after filtrate spray drying；The chicken feet hide collagen raw material is also wrapped
Include the filtrate dry powder.
In the method for preparing chicken feet hide collagen raw material that the present invention is provided, the chicken feet hide collagen raw material is made
For protein feed raw material is used in animal and fowl fodder formula.
Implement the present invention, have the advantages that：The present invention uses lipase, keratinase, HuPro
Protease is degraded to chicken feet skin, realizes native protein resource rational utilization, enzymolysis process environment friend
Good pollution-free, obtained protein feed raw material is applied to be substituted or part replacement egg in animal and fowl fodder formula
White raw material.
Brief description of the drawings
Technical scheme in order to illustrate more clearly the embodiments of the present invention, in being described to embodiment below
The required accompanying drawing for using is briefly described.In accompanying drawing：
Fig. 1 is the flow chart of the method preferred embodiment that the present invention prepares chicken feet hide collagen raw material；
Fig. 2 is the amino acid analysis collection of illustrative plates of filtrate in the embodiment of the present invention.
Specific embodiment
Below in conjunction with embodiment, the technical scheme in the embodiment of the present invention is clearly and completely retouched
State.
Research in the prior art for chicken feet skin is less, is added after directly chicken feet skin is crushed and is supported in livestock and poultry
Grow in feed, utilization rate is very low.
Main innovation point of the invention is, using lipase, keratinase, HuPro protease to chicken feet
Skin is degraded, and obtains the chicken feet hide collagen raw material rich in various amino acid, to substitute or partly substitute
Protein raw materials in animal and fowl fodder formula.
Enzymolysis process parameter includes solid-liquid ratio, hydrolysis temperature, enzymolysis pH value, addition, assistant agent, enzymolysis
Time etc..Based on solid-liquid ratio did not enough just have chicken feet skin by water energy, then carry out single factor experiment determination most
Good ratio；Hydrolysis temperature and pH value are according to the most suitable of the keratinase and HuPro protease for playing a major role
Reaction temperature and pH value determine；Addition is determined by single factor experiment；Assistant agent species and addition pass through
Single factor experiment and orthogonal test determine；Enzymolysis time is determined by single factor experiment.
Fig. 1 shows that the present invention prepares the flow of the method preferred embodiment of chicken feet hide collagen raw material, such as schemes
Shown in 1, the method that the present invention prepares chicken feet hide collagen raw material comprises the following steps：
S100, chicken feet skin is mixed with water, add lipase, keratinase, anhydrous sodium sulfite,
PH8~10,2~4h is digested at 40~50 DEG C, obtain the first enzymolysis liquid；
S200, the first enzymolysis liquid pH to 7.0~9.0 is adjusted, add HuPro protease, 50~
4~6h is digested at 70 DEG C, the second enzymolysis liquid is obtained；
S300, filtering second enzymolysis liquid, filtration cakes torrefaction are crushed, and obtain final product chicken feet hide collagen raw material.
Embodiment 1
1) preparation of raw material
Discarded chicken feet skin is collected from market, running water is cleaned up, drained, and places 80 DEG C of oven drying 12h,
Directly saved backup without crushing, or crushed standby.
2) enzymolysis process
The weight ratio of chicken feet skin and water is placed in reactor for 1: 8, a certain amount of lipase of addition, keratinase,
Anhydrous sodium sulfite, lipase active reaches 15U/mL, keratinase enzyme in making the aqueous solution of enzymatic hydrolysis system
15U/mL living, the mass fraction of sodium sulfite is 10g/L, and 4h is digested at pH8.0,40 DEG C, is digested
During, shaking table shakes or mechanical agitation or magnetic agitation, obtains the first enzymolysis liquid；
Adjust the first enzymolysis liquid pH to 7.0, add HuPro protease, in making the aqueous solution of enzymatic hydrolysis system
The enzyme activity of HuPro protease is 18.0U/mL；4h is digested at 70 DEG C, the second enzymolysis liquid is obtained.
Embodiment 2
1) preparation of raw material
Discarded chicken feet skin is collected from market, running water is cleaned up, drained, and places 80 DEG C of oven drying 12h,
Directly saved backup without crushing, or crushed standby.
2) enzymolysis process
The weight ratio of chicken feet skin and water is placed in reactor for 1: 12, adds a certain amount of lipase, keratin
Enzyme, anhydrous sodium sulfite, lipase active reaches 25U/mL, keratin in making the aqueous solution of enzymatic hydrolysis system
Enzyme enzyme activity 7.5U/mL, the mass fraction of sodium sulfite is 20g/L, and 2h is digested at pH10.0,50 DEG C,
In enzymolysis process, shaking table shakes or mechanical agitation or magnetic agitation, obtains the first enzymolysis liquid；
Adjust the first enzymolysis liquid pH to 9.0, add HuPro protease, in making the aqueous solution of enzymatic hydrolysis system
The enzyme activity of HuPro protease is 9.0U/mL；6h is digested at 50 DEG C, the second enzymolysis liquid is obtained.
Embodiment 3
1) preparation of raw material
Discarded chicken feet skin is collected from market, running water is cleaned up, drained, and places 80 DEG C of oven drying 12h,
Directly saved backup without crushing, or crushed standby.
2) enzymolysis process
The weight ratio of chicken feet skin and water is placed in reactor for 1: 10, adds a certain amount of lipase, keratin
Enzyme, anhydrous sodium sulfite, lipase active reaches 20U/mL, keratin in making the aqueous solution of enzymatic hydrolysis system
Enzyme enzyme activity 10U/mL, the mass fraction of sodium sulfite is 15g/L, and 3h, enzyme are digested at pH9,45 DEG C
In solution preocess, shaking table shakes or mechanical agitation or magnetic agitation, obtains the first enzymolysis liquid；
Adjust the first enzymolysis liquid pH to 8.0, add HuPro protease, in making the aqueous solution of enzymatic hydrolysis system
The enzyme activity of HuPro protease is 15.0U/mL；5h is digested at 60 DEG C, the second enzymolysis liquid is obtained.
Interpretation of result
In above-described embodiment 1~3 second enzymolysis liquid composition be similar to, first with obtained in embodiment 3 second
As a example by enzymolysis liquid, carry out enzymolysis product and be analyzed as follows：
Second enzymolysis liquid is filtered, and obtains filtrate and filter cake.Filtrate is entered using column front derivation high performance liquid chromatography
Row amino acid analysis, collection of illustrative plates are as shown in Fig. 2 filtrate contains 16 kinds of amino acid, the figured silk fabrics containing high level
Propylhomoserin, tyrosine, leucine, wherein essential amino acid account for 54.16%, as a result such as table 1 below：
Table 1：Amino acid analysis in enzymolysis filtrate
| Numbering | Title | Amino acid content (ug/mL) in filtrate | Content (%) |
| 1 | Asp asparatates | 78.19 | 4.55% |
| 2 | Glu glutamic acid | 49.41 | 2.88% |
| 3 | Ser serines | 47.4 | 2.76% |
| 4 | His histidines | 81.98 | 4.78% |
| 5 | Gly glycine | 103.78 | 6.05% |
| 6 | Thr threonines | 159.17 | 9.27% |
| 7 | Arg arginine | 87.19 | 5.08% |
| 8 | Ala alanine | 49.38 | 2.88% |
| 9 | Tyr tyrosine | 233.23 | 13.59% |
| 10 | Cys cystines | 56.24 | 3.28% |
| 11 | Val valines | 248.91 | 14.50% |
| 12 | Met methionine | 29.54 | 1.72% |
| 13 | Phe phenylalanines | 103.47 | 6.03% |
| 14 | Ile isoleucines | 114.53 | 6.67% |
| 15 | Leu leucines | 217.8 | 12.69% |
| 16 | Lys lysines | 56.37 | 3.28% |
Filter cake (enzymolysis residue) is dried, and chicken feet lime-preserved egg Brix is remarkably improved through In vitro digestion trial checking
With rate, as a result such as table 2 below：
Table 2：Chicken feet skin Vitro Digestibility
| Chicken feet skin is not digested | Enzymolysis chicken feet skin | |
| Protein digestibility (%) | 43.52% | 75.46% |
| Dry digestibility (%) | 55.22% | 71.52% |
Table 2 is that enzymolysis chicken feet skin (filter cake) is carried out with chicken feet skin is not digested using two step external digestion systems
The analysis of protein digestibility and dry digestibility, enzymolysis chicken feet hide collagen digestibility is higher by produces without enzymolysis
The 31.94% of product, dry digestibility is also higher by 16.3%, it is seen that enzymolysis is sharp to improving the albumen of chicken feet skin
With rate.
Filtrate dry powder is obtained after filtrate spray drying, filter cake is crushed and obtains filter cake dry powder, filtrate dry powder and filter
Cracker meal can be applied to be substituted or part replacement egg in animal and fowl fodder formula as protein feed raw material
White raw material.
It should be strongly noted that the analysis method in various embodiments of the present invention is as follows：
Filtered fluid amino acid analysis：Using column front derivation high performance liquid chromatography；
Enzymolysis residue carries out the measure of protein content：Using Kjeldahl's method；
Lipase activity：With reference to GB/T23535~2009；
Keratinase enzyme activity：With reference to SHY~2015101600；
HuPro protease：With reference to GB/T 23527~2009.
Obviously, embodiment described above is only a part of embodiment of the invention, rather than whole realities
Apply example.Based on the embodiment in the present invention, those of ordinary skill in the art are not making creative work
On the premise of the every other embodiment that is obtained, belong to the scope of protection of the invention.
Claims (7)
1. a kind of method for preparing chicken feet hide collagen raw material, it is characterised in that comprise the following steps：
S100, chicken feet skin is mixed with water, add lipase, keratinase, anhydrous sodium sulfite,
PH8~10,2~4h is digested at 40~50 DEG C, obtain the first enzymolysis liquid；
S200, the first enzymolysis liquid pH to 7.0~9.0 is adjusted, add HuPro protease, 50~
4~6h is digested at 70 DEG C, the second enzymolysis liquid is obtained；
S300, filtering second enzymolysis liquid, filtration cakes torrefaction are crushed, and obtain final product chicken feet hide collagen raw material.
2. the method for preparing chicken feet hide collagen raw material according to claim 1, it is characterised in that institute
State in step S100, chicken feet skin is 1: 8~1: 12, the enzyme of lipase in the aqueous solution with the weight ratio of water
Enzyme activity for 15~25U/mL, keratinase living is 7.5~15U/mL, the matter of aqueous solution sulfite sodium
Amount fraction is 10~20g/L；In enzymolysis process, shaking table shakes or stirs.
3. the method for preparing chicken feet hide collagen raw material according to claim 1, it is characterised in that institute
State in step S200, the enzyme activity of HuPro protease is 9.0~18.0U/mL in the aqueous solution；Enzymolysis process
In, shaking table shakes or stirs.
4. the method for preparing chicken feet hide collagen raw material according to claim 1, it is characterised in that institute
State in step S300, filter second enzymolysis liquid, asparatate, glutamic acid, silk are contained in filtrate
Propylhomoserin, histidine, glycine, threonine, arginine, alanine, tyrosine, cystine, figured silk fabrics ammonia
Acid, methionine, phenylalanine, isoleucine, leucine, lysine, wherein essential amino acid contain
Amount is more than 50%.
5. the method for preparing chicken feet hide collagen raw material according to claim 1, it is characterised in that institute
Also include before stating step S100：
S001, discarded chicken feet skin is collected from market, clean up, drain, directly used after oven drying
Or crush standby.
6. the method for preparing chicken feet hide collagen raw material according to claim 1, it is characterised in that institute
State in step S300, filter second enzymolysis liquid, filtrate dry powder is obtained after filtrate spray drying；It is described
Chicken feet hide collagen raw material also includes the filtrate dry powder.
7. the method for preparing chicken feet hide collagen raw material according to any one in claim 1~6,
Characterized in that, the chicken feet hide collagen raw material is used in animal and fowl fodder formula as protein feed raw material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511028517.2A CN106922954B (en) | 2015-12-31 | 2015-12-31 | Method for preparing chicken foot skin protein raw material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511028517.2A CN106922954B (en) | 2015-12-31 | 2015-12-31 | Method for preparing chicken foot skin protein raw material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106922954A true CN106922954A (en) | 2017-07-07 |
| CN106922954B CN106922954B (en) | 2020-01-21 |
Family
ID=59442017
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511028517.2A Active CN106922954B (en) | 2015-12-31 | 2015-12-31 | Method for preparing chicken foot skin protein raw material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106922954B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114831212A (en) * | 2022-04-18 | 2022-08-02 | 武汉新华扬生物股份有限公司 | Complex enzyme preparation for enzymolysis of hoof and horn and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100892605B1 (en) * | 2007-12-18 | 2009-04-08 | 주식회사 정풍 | Producing method of extract for condiment containing much collagen from chicken feet and the extract |
| CN103255190A (en) * | 2013-05-30 | 2013-08-21 | 威海康博尔生物药业有限公司 | Method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing complex enzyme |
| CN103497943A (en) * | 2013-09-22 | 2014-01-08 | 武汉新华扬生物股份有限公司 | High-temperature-resisting neutral protease HuPro and gene and application thereof |
| CN103549109A (en) * | 2013-11-08 | 2014-02-05 | 江南大学 | Method for extracting proteins from outer skin of chicken feet |
| CN104073540A (en) * | 2014-07-05 | 2014-10-01 | 广东润科生物工程有限公司 | Polypeptide production method for enhancing utilization ratio of protein |
-
2015
- 2015-12-31 CN CN201511028517.2A patent/CN106922954B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100892605B1 (en) * | 2007-12-18 | 2009-04-08 | 주식회사 정풍 | Producing method of extract for condiment containing much collagen from chicken feet and the extract |
| CN103255190A (en) * | 2013-05-30 | 2013-08-21 | 威海康博尔生物药业有限公司 | Method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing complex enzyme |
| CN103497943A (en) * | 2013-09-22 | 2014-01-08 | 武汉新华扬生物股份有限公司 | High-temperature-resisting neutral protease HuPro and gene and application thereof |
| CN103549109A (en) * | 2013-11-08 | 2014-02-05 | 江南大学 | Method for extracting proteins from outer skin of chicken feet |
| CN104073540A (en) * | 2014-07-05 | 2014-10-01 | 广东润科生物工程有限公司 | Polypeptide production method for enhancing utilization ratio of protein |
Non-Patent Citations (1)
| Title |
|---|
| 聂新艳: ""鸡爪外皮蛋白质的提取与性质研究"", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114831212A (en) * | 2022-04-18 | 2022-08-02 | 武汉新华扬生物股份有限公司 | Complex enzyme preparation for enzymolysis of hoof and horn and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106922954B (en) | 2020-01-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11667586B2 (en) | Biological preparation method for producing amino acid liquid fertilizer from waste feathers | |
| CN103804032B (en) | Liquid amino acid compound produced by dead porcine proteins and application thereof | |
| CN108866137A (en) | Fish-skin/collagen peptide preparation method | |
| CN106031709B (en) | Milt DNA-NA, nucleoprotamine extract and preparation method thereof | |
| CN104839457A (en) | Method for preparing small peptide-enriched protein feed material based on steam explosion in combination with solid enzymatic hydrolysis | |
| CN103555804B (en) | A kind of preparation method of animals and plants mixed protein peptide | |
| Suo-Lian et al. | Technology for extracting effective components from fish scale | |
| CN103243127B (en) | Method for integrally preparing compound amino acid by using superfine grinding enzymolysis | |
| CN113481269A (en) | Preparation method of sea buckthorn protein polypeptide | |
| CN102334600A (en) | Fermented feed and preparation method thereof | |
| CN110218240B (en) | Tyrosinase inhibitory peptide and application thereof | |
| CN110408568B (en) | Bacillus licheniformis capable of producing protease in high yield and fermentation enzyme production method thereof | |
| CN101327021B (en) | Comprehensive approach of accessory product after prawn processing | |
| Mezenova et al. | Obtaining and estimating the potential of protein nutraceuticals from highly mineralized collagen-containing beef raw materials | |
| CN106922954A (en) | A kind of method for preparing chicken feet hide collagen raw material | |
| CN106222225B (en) | A kind of preparation method of ox bone active peptide | |
| CN105710107B (en) | The method and system of Compositions of Bamboo Shoot Shell high value comprehensive utilization | |
| CN104782885B (en) | The method that lactic acid hydrolyzes livestock and poultry blood | |
| CN102277403B (en) | Production technology of yellow wine lees proteins by enzymatic extraction | |
| CN104803731A (en) | Method for preparing ocean composite peptide biological fertilizer through solid state fermentation | |
| CN109295140A (en) | A kind of preparation method of Japanese croaker air bladder collagen protein source dipeptidyl peptidase-IV peptide for inhibiting | |
| CN106084000B (en) | A kind of Urechis uniconctus visceral protein source zinc chelating peptide | |
| CN103864639B (en) | A kind of N-fatty acid acylamino acid natrium surfactant and preparation method thereof | |
| CN106978460A (en) | A kind of method that utilization decapterus maruadsi processing byproduct enzymolysis prepares biologically active peptide | |
| CN107236059A (en) | Mucous membrane of small intestine accessory substance separated in synchronization extractive technique |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |