CN106914289A - Producing device of micro-fluidic chip and preparation method thereof - Google Patents
Producing device of micro-fluidic chip and preparation method thereof Download PDFInfo
- Publication number
- CN106914289A CN106914289A CN201710301713.5A CN201710301713A CN106914289A CN 106914289 A CN106914289 A CN 106914289A CN 201710301713 A CN201710301713 A CN 201710301713A CN 106914289 A CN106914289 A CN 106914289A
- Authority
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- China
- Prior art keywords
- micro
- fluidic chip
- lid
- box body
- card punch
- Prior art date
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
Abstract
The present invention relates to a kind of producing device of micro-fluidic chip and preparation method thereof.The producing device of the micro-fluidic chip includes micro-fluidic chip mould, box body, lid, card punch and support chip, and the micro-fluidic chip molding die surface is provided with the forming hole with default flow passage；The box body has forming cavity, and the bottom of the box body is used for micro-fluidic chip mould described in fixed placement；The lid can be fastened and connected with box body, and the lid is provided with lid mouth；The card punch is adapted with forming hole, and the card punch can be inserted into forming hole by covering mouth；The support chip removably on the lid, the card punch in forming hole is inserted in for support.Hole roughness that the producing device of the micro-fluidic chip makes is low, duct trans D consistent degree is high, and dregs are few in process, and small on the change influence of chip channel fluid parameter, the accuracy and precision of sample analysis is high.
Description
Technical field
The present invention relates to micro-fluidic chip field, the producing device of more particularly to a kind of micro-fluidic chip and its making side
Method.
Background technology
Micro-fluidic chip is that the basic operation units such as sample preparation, reaction, separation, detection are integrated into one piece of micro-meter scale
Chip on, be automatically performed analysis overall process, have been widely used in the research field such as biology, chemistry, medicine.Micro-fluidic chip adds
Work method mainly uses pressure sintering, injection moulding or horizontal mold method.The material of chip mainly uses silicon materials, hyaline-quartz or organic
Dimethyl silicone polymer (PDMS) in polymer, wherein organic polymer is because with good translucidus, bio-compatibility, easily
Processing and the low advantage of cost of manufacture are widely used.
It is by perforating needle manual punching hole processing and fabricating sample feeding in micro-fluidic chip process, in conventional art
The holes such as hole, sample sample outlet hole, waste liquid sample holes and waste liquid fluid hole.But, because the material of perforating needle smoothness and PDMS is special
Property, the bore edges roughness of processing and fabricating is high, and the trans D in duct is inconsistent, the absorption of sample can be caused in use or is let out
Leakage.Additionally, the dregs produced in perforating needle drill process can cause chip channel fluid parameter to change, or even chip can be blocked,
And then the accuracy and precision of sample analysis can be influenceed.
The content of the invention
Based on this, it is necessary to provide that a kind of roughness is low, duct trans D consistent degree is high and process in dregs
Few producing device of micro-fluidic chip and preparation method thereof.
A kind of producing device of micro-fluidic chip includes：
Micro-fluidic chip mould, the micro-fluidic chip molding die surface is provided with the shaping with default flow passage
Hole；
Box body, the bottom that the box body has forming cavity, the box body is used for micro-fluidic chip shaping described in fixed placement
Mould；
Lid, the lid can be fastened and connected with the box body, and the lid is provided with lid mouth；
Card punch, the card punch is adapted with the forming hole, and the card punch can be inserted into by the lid mouth
In the forming hole；And
Support chip, the support chip removably on the lid, is inserted in the forming hole for supporting
The card punch.
The producing device of the micro-fluidic chip includes micro-fluidic chip mould, box body, lid, card punch and support
Piece, the micro-fluidic chip mould fixed placement is in the bottom of the box body, the card punch and the micro-fluidic chip
Forming hole in mould is adapted, and the card punch can be inserted into the forming hole by the lid mouth.By micro-
Fluidic chip mould, box body, lid, card punch and support chip are worked in coordination, the producing device energy of the micro-fluidic chip
It is enough that sample feeding hole, sample sample outlet hole, waste liquid sample holes and waste liquid fluid hole are done directly in micro-fluidic chip forming process
The making in hole Deng needed for, relative to traditional manual punching hole, hole roughness that it makes is low, duct trans D consistent degree
Dregs are few in height, and process, and small, the accuracy and precision of sample analysis is influenceed on the change of chip channel fluid parameter
It is high.Additionally, the producing device simple structure of the micro-fluidic chip, is easy to the industrialization production of micro-fluidic chip.
Wherein in one embodiment, a diameter of 1cm-1m of the box body, the material of the box body is polypropylene and gathers
Styrene one or two.1cm-1m is set to by by the diameter of box body, it can be used in fixing the micro- of larger size range
Fluidic chip mould.Box body is easy to observe box body using polypropylene and one or two transparent material of polystyrene
In forming process, be also easy to the processing of box body.
Wherein in one embodiment, it is additionally provided between the bottom of the box body and the micro-fluidic chip mould viscous
Knot layer, the tack coat is used to fix the micro-fluidic chip mould.
Wherein in one embodiment, the material of the tack coat is dimethyl silicone polymer.Dimethyl silicone polymer is
Transparent binding material, is easy to the forming process in observation box body.
Wherein in one embodiment, the side wall of the box body is provided with altitude scale line.By being provided with altitude scale line, can
Preferably to adjust the addition of performed polymer, and then the thickness of casting micro-fluidic chip can be accurately controlled.
Wherein in one embodiment, a diameter of 1cm-1m of the lid, the material of the lid is polypropylene and gathers
Styrene one or two.
Wherein in one embodiment, the lid is connected through a screw thread and is fastened on the box body.It is connected through a screw thread, just
Installed in the assembling of box body and lid.
Wherein in one embodiment, also including three-dimensional trim holder, the three-dimensional trim holder connects the card punch, is used for
Finely tune the position during the card punch is inserted into the forming hole.By being provided with three-dimensional trim holder, being capable of comprehensive fine setting punching
Device, it is ensured that card punch is accurately inserted into forming hole, it is ensured that the making precision in hole.
Wherein in one embodiment, a diameter of 1 μm of -100mm of the card punch.
Additionally, there is a need to providing a kind of preparation method of micro-fluidic chip.
A kind of preparation method of micro-fluidic chip, uses the producing device system of micro-fluidic chip described in any of the above-described embodiment
Make, its preparation method specifically includes following steps：
By the micro-fluidic chip mould fixed placement in the bottom of the box body；
The lid is fastened and connected with the box body；
The card punch is inserted into the forming hole of the micro-fluidic chip mould by the lid mouth；
The support chip is located on the lid and support secures the above card punch；
Performed polymer is added in the forming cavity of the box body by the lid mouth；
The box body of performed polymer will be added carries out heating, drying treatment, and heating is cooled to room temperature after finishing；
After the support chip is dismantled from the lid, the card punch is extracted vertically, complete micro-fluidic chip mesopore
Making.
The hole roughness of the micro-fluidic chip that the preparation method of the micro-fluidic chip makes is low, duct trans D is consistent
Degree is high, and dregs are few in process, and small, the accuracy and precision of sample analysis is influenceed on the change of chip channel fluid parameter
It is high.Additionally, the preparation method of the micro-fluidic chip is simple to operation, it is easy to industrialization production.
Brief description of the drawings
Fig. 1 is the box body structure schematic diagram of the producing device of the micro-fluidic chip of an implementation method；
Fig. 2 is the lid structural representation in the producing device of the micro-fluidic chip shown in Fig. 1.
Description of reference numerals is as follows：
10. the producing device of micro-fluidic chip, 100. micro-fluidic chip moulds, 101. forming holes, 110. box bodys,
120. lids, 121. lid mouths, 130. card punch, 140. support chips.
Specific embodiment
For the ease of understanding the present invention, the present invention is described more fully below with reference to relevant drawings.In accompanying drawing
Give presently preferred embodiments of the present invention.But, the present invention can be realized in many different forms, however it is not limited to this paper institutes
The embodiment of description.On the contrary, the purpose for providing these embodiments is to make the understanding to the disclosure more thorough
Comprehensively.
It should be noted that when element is referred to as " being fixed on " another element, it can directly on another element
Or can also there is element placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or may be simultaneously present centering elements.
Unless otherwise defined, all of technologies and scientific terms used here by the article with belong to technical field of the invention
The implication that technical staff is generally understood that is identical.The term for being used in the description of the invention herein is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.
The producing device 10 of the micro-fluidic chip includes micro-fluidic chip shaping mould in embodiment as depicted in figs. 1 and 2
Tool 100, box body 110, lid 120, card punch 130 and support chip 140.
The surface of micro-fluidic chip mould 100 is provided with the forming hole 101 with default flow passage.Alternatively, this is micro-
Fluidic chip mould 100 can be provided with multiple forming holes 101.The forming hole 101 is processed in micro-fluidic chip
Sample feeding hole, sample sample outlet hole, waste liquid sample hole and waste liquid fluid hole etc. it is all kinds of needed for holes.
The box body 110 has forming cavity, and the bottom of the box body 110 is for the fixed placement micro-fluidic chip mould
100.The size of box body 110 is adapted with the size of micro-fluidic chip mould 100, and alternatively, the size of box body 110 is more than
The size of micro-fluidic chip mould 100.
In one embodiment, a diameter of 1cm-1m of the box body 110, the material of the box body 110 is polypropylene and polyphenyl
The transparent materials such as ethene one or two.1cm-1m is set to by by the diameter of box body 110, can be applied to box body 110
The micro-fluidic chip mould 100 of fixed size in a big way.Box body 110 using polypropylene and polystyrene etc. transparent material
One or more of material, is easy to observe the forming process in box body 110, is also easy to the processing of box body 110.
In one embodiment, it is additionally provided with bonding between the bottom of the box body 110 and the micro-fluidic chip mould 100
Layer (not shown), the tack coat is used to fix the micro-fluidic chip mould 100, it is ensured that the formed precision of micro-fluidic chip.
In one embodiment, the tack coat is using transparent materials such as dimethyl silicone polymer, polypropylene and polystyrene
In one or more.The transparent materials such as dimethyl silicone polymer, polypropylene or polystyrene are coated with by the bottom of box body 110
Material, for the micro-fluidic chip mould 100 in fixed bin body 110.
In one embodiment, the side wall of the box body 110 is provided with altitude scale line (not shown).By being provided with altitude scale
Line, can preferably adjust the addition of performed polymer, and then can accurately control the thickness of casting micro-fluidic chip.This is highly
The range of graduation mark can specifically be adjusted according to the height of box body 110.
The lid 120 can be fastened and connected with the box body 110.The size of the lid 120 is mutually fitted with the size of box body 110
Match somebody with somebody, it is ensured that lid 120 will not be moved after being fastened and connected with the box body 110.Lid 120 matches junction button together in box body by size
110.Alternatively, the lid 120 is connected through a screw thread and is fastened on the box body 110.It is connected through a screw thread, is easy to box body 110 and box
The assembling of lid 120 is installed.The lid 120 is provided with lid mouth 121.The card punch 130 can be moved in lid mouth 121, it is ensured that punching
Device 130 is accurately inserted into forming hole 101.Prepolymer liquid is used for micro-fluidic chip shaping mould of casting also by lid mouth 121
Tool 100.The position of forming hole 101 of the specific size of lid mouthful 121 in micro-fluidic chip mould 100 and prepolymer
The casting amount of liquid is adjusted, it is ensured that the movement of card punch 130, is easy to performed polymer liquid of casting.
In one embodiment, a diameter of 1cm-1m of the lid 120, the use polypropylene and polyphenyl second of the lid 120
The transparent materials such as alkene one or two.1cm-1m is set to by by the diameter of lid 120, can be applied to lid 120 and pour
Cast the micro-fluidic chip mould 100 of size in a big way.Lid 120 using polypropylene and polystyrene etc. transparent material
One or more, be easy to control box body 110 in forming process, be also easy to the processing of lid 120.
The card punch 130 is adapted with the forming hole 101, the card punch 130 can by the lid mouthfuls 121 be inserted into this into
In type hole 101.The card punch 130 can be the metal materials such as steel, can also be hard material plastics, as long as ensure that institute
Make the dimensional accuracy in hole.When forming hole 101 has multiple, card punch 130 is also to that should have multiple.Alternatively, the punching
A diameter of 1 μm of -100mm of device 130.Still optionally further, the card punch 130 is cylindrical structure.
The support chip 140 removably on the lid 120, this dozen in the forming hole 101 is inserted in for support
Hole device 130.In card punch 130 is inserted into forming hole 101, the support chip 140 is arranged on lid, for supporting this dozen
Hole device 130；When card punch needs to be transferred to from forming hole 101, the support chip 140 is removed from lid 120.The support chip
140 can use metal material, it would however also be possible to employ rigid plastics, as long as card punch 130 can be supported.The support chip
140 can support the card punch 130 by modes such as size matching or threaded connections.When card punch 130 has multiple, support chip
140 are also correspondingly provided with multiple.
In one embodiment, also including three-dimensional trim holder (not shown), three-dimensional trim holder connection card punch 130 is used
In finely tune the card punch 130 be inserted into the forming hole in position.Alternatively, the three-dimensional trim holder is detachably arranged in the lid
On 120.By being provided with three-dimensional trim holder, being capable of comprehensive fine setting card punch 130, it is ensured that card punch 130 and micro-fluidic chip into
The alignment laminating of forming hole 101 of pattern tool 100, it is ensured that the making precision in hole.
The producing device 10 of the micro-fluidic chip includes micro-fluidic chip mould 100, box body 110, lid 120, beats
Hole device 130 and support chip 140, the fixed placement of micro-fluidic chip mould 100 in the bottom of the box body 110, the card punch
130 forming hole 101 with the micro-fluidic chip mould 100 is adapted, and the card punch 130 can be by lid mouthful 121
It is inserted into the forming hole 101.By micro-fluidic chip mould 100, box body 110, lid 120, card punch 130 and support
The mutual cooperation of piece 140, the producing device 10 of the micro-fluidic chip can be done directly sample in micro-fluidic chip forming process
The making in the required holes such as product sample holes, sample sample outlet hole, waste liquid sample holes and waste liquid fluid hole, beats manually relative to traditional
Hole, its make hole roughness is low, duct trans D consistent degree is high, and dregs are few in process, to chip channel stream
Body parametric variations are small, and the accuracy and precision of sample analysis is high.Additionally, the knot of the producing device 10 of the micro-fluidic chip
Structure is simple, is easy to the industrialization production of micro-fluidic chip.
Additionally, there is a need to providing a kind of preparation method of micro-fluidic chip.
A kind of preparation method of micro-fluidic chip, is made using the producing device 10 of any of the above-described embodiment micro-fluidic chip
Make, its preparation method specifically includes following steps：
By the fixed placement of micro-fluidic chip mould 100 in the bottom of the box body 110.Alternatively, micro-fluidic chip
By being provided with containing materials such as dimethyl silicone polymer, polypropylene or polystyrene between the bottom of mould 100 and box body 110
Tack coat be adhesively fixed.
The lid 120 is fastened and connected with the box body 110, size and the size of box body 110 of the lid 120 match,
Ensure that lid 120 will not be moved after being fastened and connected with the box body 110.
The card punch 130 is inserted into the forming hole 101 of the micro-fluidic chip mould 100 by lid mouthful 121.
The card punch 130 is adapted with the forming hole 101, and adjusts card punch 130 and be directed at laminating with forming hole 101.
The support chip 140 is installed on the lid 120 and supported and fixes the card punch 130.Ensure adding pre-polymerization
During body fluid body, the card punch 130 having been fixed in forming hole 101 will not be moved.The support chip 140 can be by size
The mode such as matching or threaded connection supports the card punch 130.
Performed polymer liquid is added in the forming cavity of the box body 110 by lid mouthful 121, for the micro-fluidic core of casting
Sheetmolding mould 100.Alternatively, the performed polymer is polymer poly dimethyl siloxane (PDMS).
The box body 110 of performed polymer liquid will be added carries out heating, drying treatment, and heating is cooled to room temperature after finishing.It is optional
Ground, the box body 110 is heated 4 hours in being put into 65 DEG C of baking ovens.
After the support chip 140 is dismantled from the lid 120, the card punch 130, the hole on micro-fluidic chip are extracted vertically
Shaping is completed.The subsequent processing operations of micro-fluidic chip will be proceeded, you can complete the making of micro-fluidic chip.
The micro-fluidic chip made by the preparation method of the micro-fluidic chip, its hole roughness is low, duct laterally straight
Footpath consistent degree is high, and dregs are few in process, on chip channel fluid parameter change influence it is small, the degree of accuracy of sample analysis and
Accuracy is high.Additionally, the preparation method of the micro-fluidic chip is simple to operation, it is easy to industrialization production.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope of this specification record is all considered to be.
Embodiment described above only expresses several embodiments of the invention, and its description is more specific and detailed, but simultaneously
Can not therefore be construed as limiting the scope of the patent.It should be pointed out that coming for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of producing device of micro-fluidic chip, it is characterised in that including：
Micro-fluidic chip mould, the micro-fluidic chip molding die surface is provided with the forming hole with default flow passage；
Box body, the bottom that the box body has forming cavity, the box body is used for micro-fluidic chip mould described in fixed placement；
Lid, the lid can be fastened and connected with the box body, and the lid is provided with lid mouth；
Card punch, the card punch is adapted with the forming hole, and the card punch can be inserted into described by the lid mouth
In forming hole；And
Support chip, the support chip removably on the lid, described in supporting and being inserted in the forming hole
Card punch.
2. the producing device of micro-fluidic chip according to claim 1, it is characterised in that a diameter of 1cm- of the box body
1m, the material of the box body is one or two in polypropylene and polystyrene.
3. the producing device of micro-fluidic chip according to claim 1, it is characterised in that the bottom of the box body and described
Tack coat is additionally provided between micro-fluidic chip mould, the tack coat is used to fix the micro-fluidic chip mould.
4. the producing device of micro-fluidic chip according to claim 3, it is characterised in that the material of the tack coat is poly-
Dimethyl siloxane.
5. the producing device of micro-fluidic chip according to claim 1, it is characterised in that the side wall of the box body is provided with height
Degree graduation mark.
6. the producing device of micro-fluidic chip according to claim 1, it is characterised in that a diameter of 1cm- of the lid
1m, the material of the lid is one or two in polypropylene and polystyrene.
7. facture of microchip device according to claim 1, it is characterised in that the lid is connected through a screw thread button
Together in the box body.
8. facture of microchip device according to claim 1, it is characterised in that described also including three-dimensional trim holder
Three-dimensional trim holder connects the card punch, for finely tuning the position during the card punch is inserted into the forming hole.
9. the producing device of micro-fluidic chip according to claim 1, it is characterised in that a diameter of 1 μ of the card punch
m-100mm。
10. a kind of preparation method of micro-fluidic chip, it is characterised in that using micro-fluidic as described in claim any one of 1-9
The producing device of chip makes, and its preparation method specifically includes following steps：
By the micro-fluidic chip mould fixed placement in the bottom of the box body；
The lid is fastened and connected with the box body；
The card punch is inserted into the forming hole of the micro-fluidic chip mould by the lid mouth；
The support chip is located on the lid and support secures the above card punch；
Performed polymer is added in the forming cavity of the box body by the lid mouth；
The box body of performed polymer will be added carries out heating, drying treatment, and heating is cooled to room temperature after finishing；
After the support chip is dismantled from the lid, the card punch is extracted vertically, complete the system of micro-fluidic chip mesopore
Make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710301713.5A CN106914289B (en) | 2017-05-02 | 2017-05-02 | Producing device of micro-fluidic chip and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710301713.5A CN106914289B (en) | 2017-05-02 | 2017-05-02 | Producing device of micro-fluidic chip and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
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| CN106914289A true CN106914289A (en) | 2017-07-04 |
| CN106914289B CN106914289B (en) | 2019-10-15 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710301713.5A Active CN106914289B (en) | 2017-05-02 | 2017-05-02 | Producing device of micro-fluidic chip and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112387316A (en) * | 2019-08-19 | 2021-02-23 | 中国科学院亚热带农业生态研究所 | 3D micro-fluidic chip model, 3D micro-fluidic chip, and preparation method and application thereof |
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| US20090114293A1 (en) * | 2005-10-25 | 2009-05-07 | Masaki Kanai | Flow cell and process for producing the same |
| CN101530775A (en) * | 2009-03-03 | 2009-09-16 | 南京大学 | Micro-fluidic apparatus integrated with PDMS film, manufacturing method and application thereof |
| CN105268491A (en) * | 2015-10-26 | 2016-01-27 | 中国工程物理研究院激光聚变研究中心 | Method for preparing micro-fluidic/milli-fluidic chip by using detachable template |
| CN105665045A (en) * | 2016-02-05 | 2016-06-15 | 中国科学技术大学 | Micro-fluidic chip and preparation method thereof |
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2017
- 2017-05-02 CN CN201710301713.5A patent/CN106914289B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090114293A1 (en) * | 2005-10-25 | 2009-05-07 | Masaki Kanai | Flow cell and process for producing the same |
| CN101530775A (en) * | 2009-03-03 | 2009-09-16 | 南京大学 | Micro-fluidic apparatus integrated with PDMS film, manufacturing method and application thereof |
| CN105268491A (en) * | 2015-10-26 | 2016-01-27 | 中国工程物理研究院激光聚变研究中心 | Method for preparing micro-fluidic/milli-fluidic chip by using detachable template |
| CN105665045A (en) * | 2016-02-05 | 2016-06-15 | 中国科学技术大学 | Micro-fluidic chip and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112387316A (en) * | 2019-08-19 | 2021-02-23 | 中国科学院亚热带农业生态研究所 | 3D micro-fluidic chip model, 3D micro-fluidic chip, and preparation method and application thereof |
| CN112387316B (en) * | 2019-08-19 | 2021-09-21 | 中国科学院亚热带农业生态研究所 | 3D micro-fluidic chip model, 3D micro-fluidic chip, and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
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| CN106914289B (en) | 2019-10-15 |
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