CN106906157A - The method of Rhodococcus sp and its production flocculant and the application in kelp processing waste water - Google Patents

The method of Rhodococcus sp and its production flocculant and the application in kelp processing waste water Download PDF

Info

Publication number
CN106906157A
CN106906157A CN201710019964.4A CN201710019964A CN106906157A CN 106906157 A CN106906157 A CN 106906157A CN 201710019964 A CN201710019964 A CN 201710019964A CN 106906157 A CN106906157 A CN 106906157A
Authority
CN
China
Prior art keywords
rhodococcus
waste water
processing waste
kelp processing
kelp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201710019964.4A
Other languages
Chinese (zh)
Other versions
CN106906157B (en
Inventor
赵希岳
宋厚煜
张秀琴
蔡志强
何玉财
吴彦霏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Changzhou University
Original Assignee
Changzhou University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Changzhou University filed Critical Changzhou University
Priority to CN201710019964.4A priority Critical patent/CN106906157B/en
Publication of CN106906157A publication Critical patent/CN106906157A/en
Application granted granted Critical
Publication of CN106906157B publication Critical patent/CN106906157B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters

Abstract

Method the invention discloses Rhodococcus sp and its production flocculant and the application in kelp processing waste water, belong to microorganism in field of wastewater processing technology field.With the Rhodococcus sp with flocculation ability high that the present invention is provided（Rhodococcus）The metabolite of C3 as main active, as microbial flocculant, be applied to degraded kelp processing waste water, with it is nontoxic, can natural degradation, high treating effect, non-secondary pollution the features such as.Added under combination condition most preferably, the fermented produced microbial flocculants of bacterial strain C3 reach 88.3% to the turbidity of kelp processing waste water, wastewater treatment efficiency are improve, for the efficient process of kelp processing waste water provide new method.

Description

The method of Rhodococcus sp and its production flocculant and the application in kelp processing waste water
Technical field
The present invention relates to be sieved in a kind of sludge by starchy material processing enterprise sewage aerobic pond with height flocculation energy The bacterial strain of power, the fermented produced microbial flocculant (MBF) of the bacterial strain has efficient flocculation heavy to kelp processing waste water Shallow lake effect, belongs to microorganism in field of wastewater processing technology field.
Background technology
Used as the relatively early water treatment agent for being used, chemical floc mainly includes that inorganic flocculating agent and organic polymer flocculate Agent, this kind of flocculant has the features such as cheap, treatment effect is obvious, therefore is widely used in early stage, but uses process Easily cause metal ion and organic substance residues and cause secondary pollution.
Kelp processing waste water contains the organic matters such as polysaccharide, cellulose, protein and pigment, and a large amount of inorganic salts, also Finite concentration has virose formaldehyde.Because kelp processing industry waste water is more containing organism kinds, greatly pollution is caused to environment, passed The aeration Aerobic Process for Treatment high cost of system, takes up an area big, operation inconvenience, it is difficult to meet the wastewater treatment demand of medium-sized and small enterprises.
The content of the invention
In order to solve problem above, the present invention provides a kind of bacterial strain Rhodococcus sp Rhodococcus with flocculation ability high Sp.C3, hereinafter referred to as bacterial strain C3.The strain was preserved in the Chaoyang District, Beijing City North Star west of China on November 14th, 2016 In China Committee for Culture Collection of Microorganisms's common micro-organisms of the Institute of Microorganism, Academia Sinica of the institute 3 of road 1 The heart (CGMCC), deposit number is CGMCC NO.13268.The Classification And Nomenclature of suggestion is Rhodococcus sp Rhodococcus sp..
It is a further object of the present invention to provide a kind of microbial flocculant, using the metabolite of bacterial strain C3 as chief active Composition, is applied to kelp processing waste water, with it is nontoxic, can the spy such as natural degradation, treatment effect, non-secondary pollution Point.
Microbial flocculant produced by above-mentioned bacterial strain C3 in the application in kelp processing waste water is processed, according to following Step is carried out：
(1) above-mentioned Rhodococcus sp Rhodococcus sp. strains are accessed into fermentation medium, constant-temperature shaking incubator with 30 DEG C of temperature, rotating speed 160r/min culture 48h, 5min is centrifuged by zymotic fluid with centrifuge 5000/min, takes supernatant, is micro- life Thing flocculant.
(2) microbial flocculant and CaCl are added in kelp processing waste water2, regulation pH value 7.0-12.0.By preferred, Flocculating conditions are：In per 100mL kelp processing waste water, microbial flocculant 2mL is added；1%CaCl22mL；PH value is 9.0.
The fermentation medium of the step (1) is：Brown sugar 30g, K2HPO46.70g, KH2PO41.70g, NH4(SO4)2 2.70g, distilled water 1L, initial pH7.0,108 DEG C, sterilize 20min.
The beneficial effects of the invention are as follows：Microbial flocculant produced by bacterial strain C3 is added to kelp processing by the present invention In waste water, the suspension in waste water, colloidal solid can be assembled to form larger alumen ustum, separation of solid and liquid is realized after standing.This hair The microbial flocculant of bright offer can quickly and effectively settle organic matter, reduction turbidity in kelp processing waste water, make water outlet clear It is clear transparent.By flocculation treatment, the load of processing system is reduced, improve treatment effeciency.Added under combination condition most preferably, bacterium COD of the fermented produced microbial flocculants of strain C3 to kelp processing waste waterGr, turbidity removal rate be respectively 89.9%, 88.30%, wastewater treatment efficiency is improve, for the efficient process of kelp processing waste water provide new method.
Brief description of the drawings
Fig. 1 bacterial strain C3 growth curve charts.
The scanning electron microscope (SEM) photograph of Fig. 2 bacterial strains C3.
The transmission electron microscope picture of Fig. 3 bacterial strains C3
Fig. 4 bacterial strain C3 16Sr DNA fragmentation amplification figures.
Fig. 5 bacterial strains C3 development trees.
The flocculation activity branch situation of Fig. 6 bacterial strains C3 institutes producing microbial flocculant.
Fig. 7 bacterial strains C3 produces the influence of the dosage to flocculating effect of flocculant.
Influences of Fig. 8 pH to flocculating effect.
Specific embodiment
The acquisition of the Rhodococcus sp C3 of embodiment 1 (Rhodococcus C3) and identification
(1) screening technique of C3
Sampling spot elects starchy material processing enterprise as.Because microbial flocculant is generally macromolecular polysaccharide, and starch It is a kind of most common polysaccharide.Therefore can from starchy material processing industry obtain much starch degradation bacteria, therefrom sieve take product it is many The probability of the flocculated bacteria of sugar can be very big.Formed sediment from Jiangsu Jin Maoyuan biochemical industries Co., Ltd using dilution spread and plate streak Isolated strains in the activated sludge of silty Raw material processing aerating wastewater pond, isolate and purify and obtain 34 plants of bacterial strains, have 12 plants of bacterium through primary dcreening operation The tunning of strain can make aqueous suspension ofkaolin produce flocculation phenomenon, have floc sedimentation to produce, it was demonstrated that its tunning has flocculation special Property.The 12 plants of bacterial strains that will be obtained after primary dcreening operation carry out secondary screening, and the flocculation ability of microbial flocculant, secondary screening result are characterized with flocculating rate 1 is shown in Table, wherein, the flocculating rate of S2, S4, Z1, C1, C3 is all higher than 90%, maximum with the flocculating rate of C3, is 94.4%.
The secondary screening result of table 1
(2) measure of bacterial strain C3 growth curves
Liquid Culture based formulas are identical with screening and culturing based formulas.
Specific method：By medium sterilization and it is inoculated with, is then placed in being vibrated in shaking table, shaking speed is 160r/min, temperature It is 30 DEG C to spend, and the absorbance under 600nm wavelength, with the time as abscissa, OD are determined every 4 hours660Drawn for ordinate and given birth to Curve long.
Result is exponential phase, 48h as shown in figure 1, as seen from Figure 1, bacterial strain C3 rapidly increases in 0-16h bacterium numbers Enter the growth stage of stable development later, bacterium number is there is no violent change.
(3) morphological feature of bacterial strain C3 and strain idenfication result are as follows：
A. bacterial strain C3 monomers and colony characteristicses
Bacterial strain C3 is coccus, aerobic, unpowered, without gemma, gram-positive bacteria (Fig. 2-3).Cultivated in LB culture mediums Initial stage thalline progressively becomes elongated rod shape by sphaerocyst, continues to cultivate thalline and be progressively broken to become rod-short, and late stage of culture becomes again Globulate.On LB culture plates, bacterium colony is in orange red, opaque projection, moistening, flush edge.
B. the sequencing of the 16S rDNA of bacterial strain C3
The extraction of bacterial strain C3 STb genes：(1) strain culturing：By inoculation in 5mL fluid nutrient mediums, shaking table is put into 160r/min, 30 DEG C of cultures to exponential phase；(2) microorganism collection：1mL cultures are taken with the pipette of sterilizing in EP pipes, 6000r/min centrifugations 5min, removes supernatant at room temperature, and precipitation is redissolved in into the ddH that pH is 8.02In O；(3) thalline splits Solution：The lysozyme soln of 50mg/mL is prepared, 6 μ L is then taken and is added in solution obtained in the previous step, 2h is acted at 37 DEG C, then It is the albumen of 20mg/L to be separately added into NaCl solution, the SDS that 110 μ L concentration are 10%, 3 μ L concentration that 50 μ L concentration are 2moL/L Enzyme K, 37 DEG C overnight.(4) extract：Bacterium solution is averagely assigned in 2 EP pipes of 1.5mL, add isometric phenol for preparing, chloroform, (three's volume ratio is 25 to isoamyl alcohol:24:1), room temperature places 5-10min, 12000r/min centrifugation 10min after mixing, Extracting 2 times；(5) precipitate：Add 0.6 times of isopropanol of extract volume；Room temperature places 10min, 12000r/min after mixing Centrifugation 10min；(6) wash：The precipitation that will be obtained washs 2 times with 75% ethanol, then 12000r/min centrifugations 10min； (7) after drying, it is dissolved in the ddH of 50 μ L2In O, take 2-5 μ L carries out electrophoresis on the Ago-Gel for preparing, after electrophoresis terminates It is put into EB and dyes 20min.
Bacterial strain C3 16S rDNA are expanded：Reaction system is shown in Table 2：
The 16S rDNA amplification reaction systems of table 2
PCR amplifications need 94 DEG C of predegenerations 5min, 94 DEG C of denaturation 40s, 55 DEG C of annealing 50s, totally 35 circulations, 72 DEG C of extensions 1.5min, 72 DEG C of termination 7min.Pcr amplification product takes 10 μ L and is detected with 1% agarose gel electrophoresis.Pcr amplification product product Electrophoretogram is as shown in Figure 3.As shown in Figure 4, the 16S rDNA fragment electrophoretic bands after PCR amplifications do not have near 1600-1650bp There are non-specific band and primer dimer, as a result well.
DNA sequencing：Sequencing is completed by Sangon Biotech (Shanghai) Co., Ltd..
C. Phylogenetic analysis of bacterial strain C3
Chadogram is drawn according to 16S rDNA sequencing results, as shown in Figure 5.The strain was protected on November 14th, 2016 It is hidden in the China Microbiological bacterium of the Institute of Microorganism, Academia Sinica of the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 of China Preservation administration committee's common micro-organisms center (CGMCC) is planted, deposit number is CGMCC NO.13268.The Classification And Nomenclature of suggestion It is Rhodococcus sp Rhodococcus sp..
D. conclusion：Reference《The outstanding Bacteria Identification handbook of uncle》, with reference to 16S rDNA sequences similitude be 99.51%, identification Bacterial strain C3 is Rhodococcus sp (Rhodococcus).The strain was preserved in the court of Beijing of China on November 14th, 2016 The China Committee for Culture Collection of Microorganisms of the Institute of Microorganism, Academia Sinica of the institute 3 of positive area's North Star West Road 1 is general Logical microorganism center (CGMCC), deposit number is CGMCC NO.13268.The Classification And Nomenclature of suggestion is Rhodococcus sp Rhodococcus sp.。
(4) microbial activity distribution situation
Specific method：The kaolinite soil suspension that 100mL concentration is 4%, label 1-5, to adding 2.5mL in No. 1 are prepared respectively Fermenation raw liquid (contains cell), and 2mL concentration is 1% CaCI2Solution, adjusts pH9.0, and No. 2-No. 4 samples are by fermenation raw liquid (containing thin Born of the same parents) supernatant after centrifugation and body bacteria suspension are replaced with respectively, other conditions are constant, stir soon 5 minutes, and 5min is stirred slowly, Absorbance of the supernatant under 550nm wavelength is determined respectively after standing 6min, and No. 5 samples add 3mL distilled water as control.Knot Fruit as shown in fig. 6, it will be appreciated from fig. 6 that flocculation activity highest is fermenation raw liquid (containing cell), flocculation activity up to 95.4%, secondly Be the supernatant after centrifugation, flocculation activity up to 95.2%, flocculation activity it is minimum be thalline suspension, flocculation activity only has 29.5%, thus illustrate, the microbial flocculant main active is Extracellular metabolism, and with good thermally-stabilised Property.
The optimum flocculating condition of the treatment kelp processing industry waste water of embodiment 2 determines and applies
(1) preparation of microbial flocculant：By bacterial strain C3 access equipped with 100mL fermentation mediums (1000mL distilled water, Brown sugar 30g, K2HPO4 5.17g、KH2PO4 1.70g、NH4(SO4)22.70g, initial pH7.0,108 DEG C of sterilizing 20min) In 250mL triangular flasks, in constant-temperature shaking incubator with 30 DEG C of temperature, rotating speed 160r/min cultures 48h.Zymotic fluid is used after culture Centrifuge 5000r/min is centrifuged 3min, takes supernatant as microbial flocculant.
(2) single factor test optimum flocculating condition experiment：
A. influence experiment of the microbial flocculant dosage to flocculating effect
In 100mL starchy material processing waste waters, fixed 1%CaCl2Consumption is 2mL, pH7.0, compares microorganism wadding Flocculating effect when solidifying agent dosage is 0.5mL, 1.0mL, 1.5mL, 2.0mL, 2.5mL, 3.0mL, 3.5mL, as a result such as Fig. 7 institutes Show, as shown in Figure 7, the flocculating rate highest when flocculant dosage is 3mL, up to 93.0%.
Influence experiment of the B.pH values to flocculating effect
The change of pH value directly affects the zeta potential in water body, and then influences the combination of biological flocculant and insoluble matter, because The size of this pH value is the principal element for influenceing flocculating effect.In 100mL starchy material processing waste waters, fixed 1%CaCl2 Consumption is 2mL, compares flocculating effect when pH is respectively 6,7,8,9,10,11, as a result as shown in figure 8, as shown in Figure 8, working as pH Be worth for 9.0 when can reach highest flocculating rate for 93.2%.
C.1%CaCl2Experiment of the dosage to flocculating effect
In 100mL kelp processing waste water, fixed 1%CaCl2Consumption is 2mL, and pH is 9.0, compares 1%
CaCl2Dosage is respectively flocculating effect when 1mL, 2mL, 3mL, 4mL, 5mL, as a result understands, works as 1%CaCl2Throw Flocculating rate has been up to 94.8% when dosage is 2mL.
From single factor experiment result, optimal flocculation bar of the microbial flocculant to kelp processing waste water flocculating effect Part is：In per 100mL kelp processing waste water, microbial flocculant 2mL, 1%CaCl are added22mL, pH9.0.
(4) example
According to draw optimum optimization dosage (in per 100mL kelp processing waste water, add microbial flocculant 3mL, 1%CaCl22mL, pH9.0), treatment effect experiment, measurement result such as table 3 are carried out to kelp processing waste water.
The clearance of every physical and chemical index under the optimum optimization treatment conditions of table 3
Testing index Before processing After treatment Clearance (%)
0.288 0.032 88.9
784 79 89.9

Claims (3)

1. Rhodococcus spRhodococcusSp., deposit number is CGMCC NO. 13268.
2. the microbial flocculant produced by above-mentioned Rhodococcus sp is in the application in kelp processing waste water is processed, it is characterised in that Peace is carried out according to following step：
（1）By above-mentioned Rhodococcus spRhodococcusSp. strain accesses fermentation medium, in constant-temperature shaking incubator with temperature 30 DEG C, zymotic fluid is centrifuged 5min by rotating speed 160r/min culture 48h with centrifuge 5000/min, takes supernatant, is microorganism wadding Solidifying agent；（2）Microbial flocculant and CaCl are added in kelp processing waste water2, pH value 7.0-12.0 is adjusted, flocculating conditions are： In per 100mL kelp processing waste water, microbial flocculant 2mL is added；1% CaCl2 2mL；PH value is 9.0.
3. the microbial flocculant produced by Rhodococcus sp according to claim 2 is in answering in kelp processing waste water is processed With, it is characterised in that the step（1）Fermentation medium be：Brown sugar 30g, K2HPO46.70g, KH2PO41.70g, NH4 (SO4)22.70g, distilled water 1L, initial pH7.0,108 DEG C, sterilize 20min.
CN201710019964.4A 2017-01-12 2017-01-12 Rhodococcus, method for producing flocculant by using same and application of rhodococcus in kelp processing wastewater Active CN106906157B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710019964.4A CN106906157B (en) 2017-01-12 2017-01-12 Rhodococcus, method for producing flocculant by using same and application of rhodococcus in kelp processing wastewater

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710019964.4A CN106906157B (en) 2017-01-12 2017-01-12 Rhodococcus, method for producing flocculant by using same and application of rhodococcus in kelp processing wastewater

Publications (2)

Publication Number Publication Date
CN106906157A true CN106906157A (en) 2017-06-30
CN106906157B CN106906157B (en) 2020-09-08

Family

ID=59206465

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710019964.4A Active CN106906157B (en) 2017-01-12 2017-01-12 Rhodococcus, method for producing flocculant by using same and application of rhodococcus in kelp processing wastewater

Country Status (1)

Country Link
CN (1) CN106906157B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109956562A (en) * 2019-04-23 2019-07-02 常州大学 For handling the preparation of the Rhodococcus sp and its biological flocculant of kelp processing waste water
CN110016449A (en) * 2019-04-25 2019-07-16 常州大学 A kind of Rhodococcus sp high-efficiency fermenting culture medium and the cultural method that Rhodococcus sp is cultivated with it
CN112340857A (en) * 2020-11-03 2021-02-09 苏州汉风科技发展有限公司 Environment-friendly efficient wastewater treatment process

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101857846A (en) * 2010-04-20 2010-10-13 深圳清华大学研究院 Rhodococcus erythropolis and microbial inoculum and application thereof
CN103602620A (en) * 2013-11-28 2014-02-26 辽宁大学 High-flocculation capability bacterial strain and use thereof
CN103667108A (en) * 2013-10-17 2014-03-26 浙江省环境保护科学设计研究院 Rhodococcus corynebacterioides strain and application thereof in printing and dyeing wastewater treatment

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101857846A (en) * 2010-04-20 2010-10-13 深圳清华大学研究院 Rhodococcus erythropolis and microbial inoculum and application thereof
CN103667108A (en) * 2013-10-17 2014-03-26 浙江省环境保护科学设计研究院 Rhodococcus corynebacterioides strain and application thereof in printing and dyeing wastewater treatment
CN103602620A (en) * 2013-11-28 2014-02-26 辽宁大学 High-flocculation capability bacterial strain and use thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GUO JY等: "Treatment of swine wastewater using chemically modified zeolite and bioflocculant from activated sludge", 《BIORESOURCE TECHNOLOGY》 *
QY LIUA等: "The Isolation of Bioflocculant-producing Strains and the Application in the Oily Wastewater Treatment Process", 《PETROLEUM SCIENCE AND TECHNOLOGY》 *
邱国良: "厌氧后猪场废水资源化制备微生物絮凝剂", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 *
郭俊元等: "淀粉废水生产微生物絮凝剂及发酵动力学特征", 《中国环境科学》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109956562A (en) * 2019-04-23 2019-07-02 常州大学 For handling the preparation of the Rhodococcus sp and its biological flocculant of kelp processing waste water
CN110016449A (en) * 2019-04-25 2019-07-16 常州大学 A kind of Rhodococcus sp high-efficiency fermenting culture medium and the cultural method that Rhodococcus sp is cultivated with it
CN112340857A (en) * 2020-11-03 2021-02-09 苏州汉风科技发展有限公司 Environment-friendly efficient wastewater treatment process

Also Published As

Publication number Publication date
CN106906157B (en) 2020-09-08

Similar Documents

Publication Publication Date Title
CN101503709B (en) Method for preparing bioflocculation by Bacillus licheniformis
CN101225405A (en) Method for producing microbial flocculant and method of use thereof
Wang et al. Synthetic white spirit wastewater treatment and biomass recovery by photosynthetic bacteria: Feasibility and process influence factors
Bao-jun et al. The research status and development trend of microbial flocculant
CN106906157A (en) The method of Rhodococcus sp and its production flocculant and the application in kelp processing waste water
CN103602620B (en) A kind of bacterial strain and application thereof with high flocculation ability
CN110218682B (en) Pseudomycosis bacillus and application thereof in sludge reduction
CN101070203A (en) Method for preparing biological flocculant using strange deformation bacilli
CN111268805B (en) Bacillus cereus and application thereof in pig manure biogas slurry flocculation
CN113604407A (en) Composite microbial algaecide and preparation method and application thereof
CN115786192B (en) Bacillus paramycoides and application thereof
CN105349462B (en) One plant of American aloe bacillus Hexi1 and its application in compost
CN114292797B (en) Physarum viscosum and application of microbial flocculant thereof in sewage treatment
CN104560822A (en) Psychrotolerant bacterium with high flocculation activity and with decolorization on methylene blue
CN106591173A (en) Bacillus flexus HL-37 capable of activating soil heavy metal cadmium, and applications thereof
CN113755405B (en) Bacillus bacteria F12 and application thereof
CN111233167B (en) Alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation
CN104556405B (en) A kind of Biological compound flocculant containing ferric ion and application thereof
CN111621437B (en) Otter escherichia coli LM-DK separated from oxidation pond of pig farm and application thereof
CN114162978A (en) Application of cryptococcus oxytetracycline in wastewater treatment
CN114317302A (en) Black and odorous water body remediation microbial inoculum and application thereof
CN116064309B (en) New bacterial strain of pseudomonas and application thereof
CN116536222B (en) Stenotrophomonas palum and application thereof in dye wastewater treatment
CN109956562A (en) For handling the preparation of the Rhodococcus sp and its biological flocculant of kelp processing waste water
CN114134076B (en) Bacillus licheniformis for producing extracellular polysaccharide, flocculant and application of flocculant in sewage treatment

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant