CN106900763A - A kind of pig farm special bio thimerosal - Google Patents

Abstract

The present invention relates to a kind of pig farm special bio thimerosal, belong to disinfecting articles for use, formed using pure natural oceanic plant, herbaceous plant extract and guanidine material compounding.Present invention is mainly used for the prophylactic disinfection treatment of pig breeding farm, including house of such as being bred to various special pig houses, gestation house, childbirth house, piglet educates society, grows prophylactic disinfection, the sterilization of band pig, feed trough sterilization, swinery sterilization, disinfection of drinking water, air sterillization, the feed disinfection of pig house.Has a broad antifungal spectrum of the invention, sterilizing ability is strong, and onset time is short, applied widely, good stability, nontoxic non-stimulated, free from extraneous odour, noresidue are degradable, the odorant in pig house air and rubbish sepage can be neutralized, the mosquitos and flies density of pig house, containment malignant bacteria, the propagation of virus is reduced.The common disease of the pigs such as effective preventing swine influenza, Streptococcus suis, hog cholera, haemophilus parasuis infection disease, reduces pig illness rate, is a kind of special environmentally friendly biological disinfectant in pig farm.

Description

A kind of pig farm special bio thimerosal

Technical field belongs to disinfecting articles for use, the master of formula the present invention relates to a kind of pig farm special bio thimerosal Composition is wanted for thick branch cartilage algae extract solution, U. pertusa extract solution, polyhexamethylene list guanidine, Banlangen Polysaccharide, hawthorn infusion, molten Bacterium enzyme, polyoxyethylene sorbitan monooleate, polyethylene glycol, are prepared from through special process.Present invention is mainly used for life The prophylactic disinfection treatment of pig plant, including house of such as being bred to various special pig houses, gestation house, childbirth house, piglet educates society, raw The prophylactic disinfection of pig house long, the sterilization of band pig, feed trough sterilization, swinery sterilization, disinfection of drinking water, air sterillization, feed disinfection.This hair Bright has a broad antifungal spectrum, sterilizing ability is strong, to various common pathogenic entero becterias, fungal spore, pyogenic baeceria, disease in pig breeding farm Poison, parasitic ovum have stronger killing action, so that the route of transmission of pathogenic microorganisms is blocked, effective preventing swine influenza, pig The common disease of the pigs such as streptococcosis, hog cholera, haemophilus parasuis infection disease, reduces pig illness rate.It is empty that the present invention can neutralize pig house Odorant in gas and rubbish sepage, reduces the mosquitos and flies density of pig house, containment malignant bacteria, the propagation of virus.With antibacterial Spectrum is wide, and onset time is short, applied widely, and good stability, purify air is deodorant, nontoxic non-stimulated, and protection effect is good, is as good as Taste, noresidue, are a kind of special environmentally friendly biological disinfectants in pig farm the characteristics of degradable.

In the disease of background technology pig farm based on bacterial disease and virosis, wherein bacterial disease is so that the incidence of disease is high, drug resistance Enhancing, clinical symptoms pathological anatomy are not true to type, diagnoses difficulty increasing and prevent and treat the aspects such as difficulty and seriously threaten pig industry Healthy and sustainable development.Chen Huanchun points out that the virosis of current pig industry has tended to relaxing, but bacteriosis is in scale pig There is ascendant trend field, and in the provinces and cities of coastal area 19 separate the bacterium bacterial strain of identification, streptococcus accounts for 29.66% （1850/6238）, haemophilus parasuis accounts for 20.54%（1281/6238）, enteropathogenic E. Coli accounts for 12.54%（782/ 6238）, Pasteurella accounts for 6.53%（396/6238）, it is other Actinobacillus, Pasteurella, Salmonella choleraesuls, golden yellow The pig such as color staphylococcus source pathogenic bacteria also endanger the development of pig industry.He Qi etc. report Escherichia coli, Salmonella choleraesuls, The pig such as staphylococcus aureus, bacillus, Shiga bacillus, haemophilus parasuis source pathogenic bacteria equally annoying Kweiyang and support The development on pig farm.Jiang Wanfeng report scales pig farm five kinds of main bacteria diseases of prevalence, including Streptococcus suis, the bloodthirsty bar of secondary pig Bacterium is sick, highly pathogenic colibacillosis, disease of also breathing and infectious pleurisy.The basic principle of pig epidemic prevention and control is " prevention Based on, anti-overweighting is controlled ", only according to different physiology, the growth phase of pig, science feeding and management is carried out, and keep pig house to clean Health, well-ventilated, disinfectant measure are proper, just can guarantee that the healthy aquaculture of pig.

Sterilized using disinfectant in cultivation, have become an indispensable measure of pig epidemic prevention and control.Country poultry The four-stage with the development experience of disinfectant.The disinfectant of first stage has that effect is certain, excitant is strong, stability is poor, The features such as toxicity is big, such as quick lime, plant ash, caustic soda, formaldehyde and potassium permanganate, Lysol etc..What second stage occurred disappears Toxic agent compensate for the deficiency of previous stage, such as compound coal tar acid solution, compound phenol have that excitant is smaller, the scope of application more Extensively, the features such as price is more economical.Phase III, chlorine-containing disinfectant, hundred poisonings with DCCNa preparation as representative were as generation Based on the quaternary ammonium salt disinfectant of table, the disinfectant stability in this stage is strong, and very little is influenceed by pH values, and to bacterium, fungi, The pathogenic microorganisms such as virus have powerful killing action.Fourth stage is mainly the composite disinfection agent of exploitation, with efficient The features such as property, broad spectrum activity, security, hypotoxicity.The compound preparation that such as quaternary ammonium salt is prepared with glutaraldehyde, formaldehyde and glutaraldehyde, It is mainly used in killing salmonella, ETEC, staphylococcus aureus, PCV-II（PVC-2）Deng pathogenic microorganism； The such as compounding such as iodine, Compound-acid, surfactant or iodine and sour compound preparation, such as Bai Sheng -30；Such as by potassium hydrogen persulfate Compound, organic acid, surfactant etc. compounding multiple-factor novel environment friendly disinfectant, represent disinfectant defend can, Wei An. These miscellaneous disinfectants all cut both ways, and peroxide disinfectant has preferably killing to bacterium, gemma, mould Effect, but its unstable chemcial property, excitant are strong, especially have stimulation to mucous membrane.Chlorinated product wide sterilization spectrum, antibiotic property It is good but little to the effect of virus, and property is unstable, its metabolite chloroform has height carcinogenicity.Operand Biological degradability is poor, welding, and bases disinfectant is sterilized, effect of killing the virus is good but big to skin and mucous membrane irritation, it is impossible to be used for Metal.The surfactant toxicity such as hundred poisonings are low, and excitant is small, but its antibacterial range is small, and incompatibility is more.Aldehydes, phenol The bactericidal properties of class disinfectant are good, but cumulative toxicity is big, high carcinogenic.Alcohols sterilizing machine sterilizing power is medium, to gemma, fungi, virus Invalid, stability is poor, volatile.In sum, existing all kinds of poultrys disinfectant has the shortcomings that different degrees of and side effect, Use that should not be excessive for a long time.The toxicity of disinfectant, excitant, corrosivity, the harmfulness of environmental pollution and to operator The harm of member's health and animal food drug residue have been increasingly becoming that the public is of concern to be asked to the safety problem of consumer Topic.And past attempts are considered as low toxicity or some nontoxic disinfectants, having been found that in recent years under certain condition still has quite Toxicity, side effect.Therefore, it is badly in need of a kind of new, safety thimerosal to overcome the bottleneck problem of the above.

Contain substantial amounts of bioactivator with the development of biotechnology, in marine organisms constantly to be extracted and ground Study carefully.Seaweed extracted liquor, biology enzyme, protein matter are marine biomaterial that is widely studied at present and using, these things Matter has excellent performance at aspects such as sterilized, antiviral, expelling parasite, gram lifes.Marine alga is inexhaustible in ocean Living resources, thick branch cartilage algae is widely distributed in south China marine site, and biomass is larger, belongs to advantage algae kind.Thick branch cartilage algae In domoic acid for drive ascarid active component, it is a kind of new natural amino acid of tricarboxylic acids, is also to kill fly active ingredient, belong to stomach Toxicity insecticide, 1% solution can kill all experimentss housefly in 24h.U. pertusa polysaccharide contains sulfate mostly, and disease-resistant Toxic action and sulfate content into positive correlation.Puritan filler acetify the antiviral activity of polysaccharide and its sulfate group and its content, The size of molecular weight is relevant.U. pertusa polysaccharide calcium composition（CaSP）Can Selective depression duplication of the virus in host cell and Propagate, the superfluous compound of the calcium ion for being formed and sulfate radical are that in host cell necessary to antiviral effect, CasP can press down The duplication of a small number of envelope virus of system.In addition to antiviral, scientific research personnel has found that algal polysaccharides also have very strong antibacterial activity, right Various common pathogens, fungal spore are respectively provided with killing action.In addition to sea-plant, herbaceous plant also have good antibacterial, The characteristic of antiviral, antimycotic, anti-gemma.In SARS epidemic, Radix Isatidis receives numerous doctors as antiviral main force's Chinese medicine The high praise energetically of medicine worker, scientific experiment shows, Radix Isatidis has preferable antibacterial, antiviral as medicinal plant really Effect.It is also many that there is the medical herb for being similar to effect in addition to Radix Isatidis.In sum, the present invention intends from various natural Bioactivator with have a safety feature, the excellent guanidine disinfectant of bactericidal effect is compounded, and length can be taken by reasonable compatibility Benefit is short, the pharmacological action of various natural active matters is performed to maximum, so as to timely and effectively cut off the biography of pathogenic microorganism Approach is broadcast, prevents the generation of swine disease.Has a broad antifungal spectrum of the invention, sterilizing ability is strong, and onset time is short, Disinfection Effect reliability, fits Wide with scope, purify air is deodorant, can reduce the content of odorant in environment, keeps pig house with fresh air, reduces mosquitos and flies Density, suppresses the breeding of pathogenic microorganism.There is good stability, nontoxic non-stimulated, diseases prevention effect compared with other disinfectant products Really good, free from extraneous odour, noresidue are degradable, the characteristics of non-secondary pollution, can effectively preventing swine influenza, Streptococcus suis, pig be suddenly The common disease of the pigs such as unrest, haemophilus parasuis infection disease.

The invention provides a kind of pig farm special bio thimerosal, it is characterized by using thick branch cartilage algae the content of the invention Extract solution, U. pertusa extract solution, polyhexamethylene list guanidine, Radix Isatidis infusion, hawthorn infusion, lysozyme, polyoxyethylene take off Water sorbitol monooleate, polyethylene glycol, are prepared from by special process.A kind of formula of pig farm special bio thimerosal is such as Under（Component and percentage by weight %）：Thick branch cartilage algae extract solution 5-8, U. pertusa extract solution 4-10, polyhexamethylene list guanidine 0.3- 1.2nd, Radix Isatidis infusion 2-7, hawthorn infusion 4-9, lysozyme 1-3, polyoxyethylene sorbitan monooleate 4-8, poly- Ethylene glycol 6-13, deionized water add to 100.

U. pertusa belongs to Chlorophyta Shi Water-shield category, is commonly called as extra large spinach, sea lettuce etc., and it is coastal to be distributed widely in Western Pacific, is Extremely abundant one kind of resource in China wild economic algae, it is maximum in yellow Bohai Sea yield.UPCM40 eggs in U. pertusa frond There is activity of resisting tobacco mosaic virus higher in vain, have a certain degree of suppression to sickle-like bacteria, Rhizoctonia solani Kuhn, musae Growth processed, its ethyl acetate extract has good inhibiting effect to staphylococcus aureus.U. pertusa has higher Gram liveliness proof, have strong inhibitory action to the growth of Heterosigma akashiwo, Skeletonema Costatum, Alexandrium tamarense, Heterosigma akashiwo cell can also be completely killed under higher concentration.The hydrotrope and methanolic extract of U. pertusa are to ocean Prorocentrum With strong growth inhibition effect.Thick branch Chondria is in Rhodophyta, Rhodophyceae, Ceramiale, Rhodomelaceae, Chondria There is marine alga, its full algae antitumor, antiviral, antimycotic, cell toxicant and expelling parasite etc. to act on.

Used as main disinfection component, the component is the cation group-poly of positively charged to polyhexamethylene list guanidine Body, with activity very high, there is very strong compatibility to microorganism, therefore it is generally in electronegative all kinds of bacteriums, true to be easy to Bacterium, virus are adsorbed, so as to suppress their schizogamy function；In addition the film that condensate is formed, plugs microorganism Respiration channel, makes microorganism death by suffocation.

Radix Isatidis has effect to various bacteria, and infusion is to hay bacillus, staphylococcus aureus, sarcine, large intestine Bacillus, typhoid bacillus, paratyphoid A bacillus, dysentery（Will Hayes, Freund）Bacillus, bacillus enteritidis etc. have inhibitory action；And it is right Hemolytic streptococcus and A type meningococcus are effective.Radix Isatidis also has the effect for killing Leptospira, can effectively prevent Leptospirosis Generation.

The main component of hawthorn is flavonoids and organic acid compound.Flavone compound mainly has Vitexin, quercitrin Element, quercitin, Hyperoside, 37, the glucoside of 4 ', 5,7 kaempferol 1 and rutin；Organic acid mainly has hawthorn Acid, citric acid, ursolic acid etc..Hawthorn infusion is to Shigella, proteus, EHEC, staphylococcus aureus etc. There is stronger bacteriostasis, its sour environment can hinder pathogenic bacteria, fungi, the growth metabolism of virus, so as to kill cause Characteristic of disease microorganism.Organic acid in hawthorn can be very good to dissolve algal polysaccharides, and adjust pH value of the invention, make this thimerosal Antiseptic property it is more reliable and more stable.

Lysozyme, also known as muramidase or N-acetylmuramide lycanohydrlase, is to stick polysaccharide during one kind can hydrolyze pathogenic bacteria Alkaline enzyme.Main β-Isosorbide-5-Nitrae glycosidic bond by destroying between the -acetylmuramic acid in cell membrane and NAG, The insoluble glutinous polysaccharide of cell membrane is resolved into soluble glycopeptide, cause cell wall rupture content to escape and make bacterolysis.It is molten Bacterium enzyme can also with negatively charged virus protein directly in conjunction with, with DNA, RNA, apoprotein formed double salt, make virally inactivated. Therefore, there is the enzyme antibacterial, anti-inflammatory, antiviral etc. to act on.

Polyoxyethylene sorbitan monooleate also known as Tween 80, it is soluble in water, it is nonionic surface active agent, tool There is emulsification, can be used as the wetting agent of supensoid agent.In the present invention, before polyoxyethylene sorbitan monooleate mainly dissolves All kinds of antimicrobial components stated, make thimerosal reach saturation,

Polyethylene glycol is nontoxic non-stimulated, with good water solubility, and has good intermiscibility with many organic constituentses, has Excellent lubricity, moisture retention, dispersiveness, antistatic behaviour and flexibility etc., play a role in this formula mainly as solvent.

Preparation technology of the invention is：（1）It is prepared by seaweed extracted liquor：The component U. pertusa and thick branch cartilage algae are washed Only, dry, with 95% alcohol dipping 4 times, to remove lipophilic composition；Then dipping 1h is added water, is boiled half an hour, filtered, it is residual Slag repeated impregnations, boil, filter 23 time, merge 4 Aqueous extracts, water-bath is concentrated into small size；

（2）The preparation of plant infusion：The component Radix Isatidis and hawthorn are cleaned, dry, crush, and half is boiled after the dipping 2h that adds water Hour, filtering, residue repeated impregnations, boil, filter merge Aqueous extracts；Using pure with analysis is steamed again under apparatus,Soxhlet's, normal pressure 40 DEG C of refluxing extraction 6h of ether, 40 DEG C of extract solution normal pressure is concentrated into small size；

（3）By proportioning by the thick branch cartilage algae extract solution of the component, U. pertusa extract solution, Radix Isatidis infusion, hawthorn infusion, Polyoxyethylene sorbitan monooleate is added in deionized water, is stirred；

（4）By proportioning by the component polyhexamethylene list guanidine, the above-mentioned mixed liquor of polyethylene glycol addition, stir, heat To 60 DEG C, 20min is emulsified；

（5）After liquid temperature degree to be mixed is down to normal temperature, during the component lysozyme added into above-mentioned mixed liquor by proportioning, deionized water 100 are added to, aseptic subpackaged, storage preservation after the assay was approved is carried out after being well mixed.

The beneficial effects of the invention are as follows：

（1）Has a broad antifungal spectrum, sterilizing ability is strong, to various common pathogenic entero becterias, fungal spore, suppuration in pig breeding farm Bacterium, virus, parasitic ovum have stronger killing action, Disinfection Effect reliability, effective preventing swine influenza, Streptococcus suis, pig The common disease of the pigs such as cholera, haemophilus parasuis infection disease, reduces pig illness rate；（2）Sterilization onset time is short, can rapidly kill cause Characteristic of disease bacterium and virus, reduce the spread speed of invasive organism；（3）Good stability, 2 years Disinfection Effects of storage remain to reach More than 99% killing rate；（4）The odorant in pig house air and rubbish sepage can be neutralized, with significant deodorant catharsis, The mosquitos and flies density of waste deposit point can effectively be reduced；（5）Pure-natural biological active component, it is nontoxic to have no stimulation, it is degradable, Secondary pollution is not produced to environment；（6）Using in extensive range, can be used for the prophylactic disinfection treatment of pig breeding farm, including it is right Various special pig houses are such as bred house, and gestation house, childbirth house, piglet educates society, grown the prophylactic disinfection of pig house, the sterilization of band pig, are fed The instrument of supporting sterilization, disinfection of drinking water, air sterillization, feed disinfection；（7）Thimerosal abundant raw material, configures simple and easy to do, low cost, It is adapted to promote the use of.

The present invention is described in further detail with reference to embodiment for specific embodiment：

1st, according to data listed in Table（Percentage by weight %）And its affiliated step is configured：

Formula one：

Thick branch cartilage algae extract solution 5-8, U. pertusa extract solution 4-10, polyhexamethylene list guanidine 0.3-1.2, Radix Isatidis infusion 2- 7th, hawthorn infusion 4-9, lysozyme 1-3, polyoxyethylene sorbitan monooleate 4-8, polyethylene glycol 6-13, deionized water Add to 100.

Formula two：

Thick branch cartilage algae extract solution 6-8, U. pertusa extract solution 7-10, polyhexamethylene list guanidine 0.6-1.2, Radix Isatidis infusion 3- 7th, hawthorn infusion 5-9, lysozyme 2-3, polyoxyethylene sorbitan monooleate 5-8, polyethylene glycol 7-13, deionized water Add to 100.

Formula three：

Thick branch cartilage algae extract solution 5-7, U. pertusa extract solution 4-8, polyhexamethylene list guanidine 0.3-1.0, Radix Isatidis infusion 2- 6th, hawthorn infusion 4-8, lysozyme 1-2, polyoxyethylene sorbitan monooleate 4-7, polyethylene glycol 6-11, deionized water Add to 100.

2nd, it is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, and Without departing from the spirit or essential characteristics of the present invention, the present invention can in other specific forms be realized.Therefore, nothing By from the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended Claim is limited rather than described above, it is intended that by the institute in the implication and scope of the equivalency of claim that falls Change and include in the present invention.

Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each implementation method is only wrapped Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should Specification an as entirety, the technical scheme in each embodiment can also be formed into those skilled in the art through appropriately combined May be appreciated other embodiment.

Claims (2)

1. a kind of pig farm special bio thimerosal, it is characterised in that using thick branch cartilage algae extract solution, U. pertusa extract solution, poly- six Methylene list guanidine, Banlangen Polysaccharide, hawthorn infusion, lysozyme, polyoxyethylene sorbitan monooleate, polyethylene glycol, It is prepared from through special process；A kind of formula of pig farm special bio thimerosal is as follows（Component and percentage by weight %）：Thick branch is soft Bone algae extract solution 5-8, U. pertusa extract solution 4-10, polyhexamethylene list guanidine 0.3-1.2, Radix Isatidis infusion 2-7, hawthorn water logging Liquid 4-9, lysozyme 1-3, polyoxyethylene sorbitan monooleate 4-8, polyethylene glycol 6-13, deionized water add to 100.

2. a kind of pig farm special bio thimerosal according to claim 1, it is characterised in that with following preparation technology：

（1）It is prepared by seaweed extracted liquor：The component U. pertusa and thick branch cartilage algae are cleaned, is dried, with 95% alcohol dipping 4 times, To remove lipophilic composition；Then add water dipping 1h, boil half an hour, filter, residue repeated impregnations, boil, filter 23 time, Merge 4 Aqueous extracts, water-bath is concentrated into small size；

（2）The preparation of plant infusion：The component Radix Isatidis and hawthorn are cleaned, dry, crush, and half is boiled after the dipping 2h that adds water Hour, filtering, residue repeated impregnations, boil, filter merge Aqueous extracts；Using pure with analysis is steamed again under apparatus,Soxhlet's, normal pressure 40 DEG C of refluxing extraction 6h of ether, 40 DEG C of extract solution normal pressure is concentrated into small size；

（3）By proportioning by the thick branch cartilage algae extract solution of the component, U. pertusa extract solution, Radix Isatidis infusion, hawthorn infusion, Polyoxyethylene sorbitan monooleate is added in deionized water, is stirred；

（4）By proportioning by the component polyhexamethylene list guanidine, the above-mentioned mixed liquor of polyethylene glycol addition, stir, heat To 60 DEG C, 20min is emulsified；

（5）After liquid temperature degree to be mixed is down to normal temperature, during the component lysozyme added into above-mentioned mixed liquor by proportioning, deionized water 100 are added to, aseptic subpackaged, storage preservation after the assay was approved is carried out after being well mixed.