CN106896095A - The micro-imaging technique of composite surface plasma resonance and surface-enhanced Raman - Google Patents
The micro-imaging technique of composite surface plasma resonance and surface-enhanced Raman Download PDFInfo
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
- G01N21/554—Attenuated total reflection and using surface plasmons detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
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Abstract
The invention discloses a kind of surface plasma body resonant vibration (Surface Plasmon Resonance, SPR) and surface-enhanced Raman（Surface‑enhanced Raman Scattering,SERS）Compound micro-imaging technique, be related to surface plasma base unit and surface-enhanced Raman field.Technical key point：Assembling SPR SERS synthesis micro imaging systems, are excited and detection SPR and SERS using nano slit array grating two mode field.Mobile judgement of the SPR system by measuring SPR peak positions excites the change of angle, to determine the change of the caused surface effective refractive index of chip surface biomolecule reaction；SERS systems are by measuring Raman spectrum directly to differentiate biomolecule in itself.And SPR and the efficient of Raman, high-sensitivity detection are by means of nano slit array grating two mode field：Excitation light wave efficiently excites SPR through optical grating construction；SPR produces dipole, the surface field that SPR is produced to obtain enhanced Raman signal with nano slit dipole collective effect enhancing surface local electric field in the communication process of metal surface with nano gap.
Description
Technical field
The present invention relates to surface plasma base unit and surface-enhanced Raman field, large area periodic nanometer gap array
Structure excites plasma resonance and surface-enhanced Raman, and a kind of composite surface plasma resonance and surface-enhanced Raman
Micro-imaging technique.
Background technology
Surface plasma resonance（Surface Plasmon Resonance, SPR）It is that photon incides precious metal surface
So as to a kind of quantum photoelectric phenomenon for causing the electronics in metal to be vibrated with electric field.SPR technique is by measuring metal circle
Occur after biological substance interacts, to excite the change of coupling condition to examine caused by the change of surface effective refractive index on face
Biomolecule is surveyed, is a kind of measurement indirectly；And Raman signal detection is then a kind of complete direct measurement.Raman scattering is to be measured
To the inelastic scattering of incident light, its essence is when photon and molecule occur inelastic collision, photon is by energy transmission for sample
After testing molecule, there is transition and radiation in molecular energy state, disclose the vibration of molecule or the spectral technique of rotational energy level.Raman light
Spectrum is there is provided the intrinsic vibration of molecule in detected materials and rotary mode, the molecular structure of direct reaction testing sample.But draw
Graceful scattering is a kind of weak scattering process, and its detecting limitation is in ambient noise and fluorescence background.Raman scattering cross section is about 10-30cm2,
And the scattering section of fluorescence process is about 10-15cm2；Relative to Raman scattering, fluorescence signal is significantly larger than Raman scattering, and this is also
The reason for fluorescent technique is more commonly used at present.Raman signal Electromagnetic enhancement is a kind of by local electric field（As coarse
Metal surface can produce enhanced local electric field）The Raman enhancement effect for being triggered；This so-called surface-enhanced Raman dissipates
Penetrate（Surface-enhanced Raman Scattering, SERS）Produced signal and photoelectricity field intensity residing for molecule
Biquadratic is directly proportional.Obviously, the enhancing of Raman signal depends on the enhancing of local electric field, and local electric field concentrates on nanometer resonance
Near structure, therefore SERS is applied to the direct resolution and detection of the protein molecular of surface attached molecules or cell surface.
In view of indirect detection and Raman spectrum direct resolution to molecule of the SPR technique to biomolecule, in recent years constantly
Having researcher to inquire into SPR Ramans strengthens, or will combine the dual-mode structure of both of which.There is now research by nano grain of silver
Embedded optical grating construction is used to the local fields between excitating surface plasma, enhancing nano particle so as to carry out surface-enhanced Raman inspection
Survey.However, what the Argent grain layer that the method is used was randomly formed, it is impossible to which precise control nano-particle position and gap are formed
Mode, therefore testing result is repeatable low, so that practical application cannot be carried out.In addition, there is research to make periodically
Gold nano butterfly junction structure forms SPR and SERS substrates；Periodic structure excitating surface plasma, and butterfly junction structure swashs
Hair dipole resonance, forms strong local electric field enhancing Raman signal.But nanostructured bowknot manufacture craft is cumbersome, it is necessary to electricity consumption
The method of beamlet photoetching or ion beam etching makes, and expensive cost of manufacture at all cannot applied generalization.Although using nanometer
Method for stamping can reduce its cost of manufacture, but its nanostructured, in transfer process, precision cannot ensure.There is research to use
111 crystal orientation carry out wet etching and obtain periodic three-legged structure on a silicon substrate, using metal periodic structure excitating surface
Plasma local electric field strengthens Raman.The method can reach 80% Raman signal detection repetitive rate, basically reach practical wanting
Ask.But its enhancement method only strengthens Raman signal by surface plasma resonance, lack nanostructured enhancing local field intensity, cause
Enhancing rate is not high, it is impossible to carry out high-precision biological detection.
The above method can to a certain extent realize that surface plasma strengthens Raman detection or surface plasma
Detected while with surface Raman enhancement, but presently, there are that cost of manufacture is high, precision is low, can poor practicability the shortcomings of；In addition,
SPR-SERS systematic collaborations carry out micro-imaging and have not been reported.
The content of the invention
The technical problems to be solved by the invention are：For above-mentioned problem, there is provided a kind of high accuracy, can be practical
Composite surface plasma resonance and surface-enhanced Raman micro-imaging technique, realize surface plasma in same chip
The super enhancing efficiently excited with surface local fields of resonance.
The technical solution adopted by the present invention is as follows：Assembling SPR-SERS synthesis micro imaging systems, using nano slit battle array
Row grating two mode field is excited and detection SPR and SERS simultaneously.
SPR-SERS synthesis micro imaging systems are as shown in Figure 1.Introduced outside simple microscope white-light illuminating light path and swashed
Light, is resonated in vitro with excitating surface etc. on SPR-SERS complex function chips, for sensing biological sample thereon；This laser
Local electric field can simultaneously be excited to be strengthened, and excitation biological sample produces SERS；This two classes signal is by micro- thing
Mirror signal collection system, light path separates and then each carries out being imaged display and data analysis.
Using large-area nano slit array optical grating construction as SPR-SERS compound chips, as shown in Fig. 2 one is all
There are two nano gaps of 10 nanometer scales in phase to produce Local field enhancement.Excitation light wave efficiently excites SPR through optical grating construction：
For microscope system, such as Fig. 3, parallel laser is focused on through microcobjective, converged on chip, reaches the incident light bag of chip
The angular aperture determined by zero degree to object lens is included, azimuth is zero degree to all light beams in 360 degree of taper；Laser light incident side
To in angular aperture and azimuthal change, there is provided the variable scanning required for SPR detections, i.e. angular aperture and azimuth angle point
Go out the blanking bar for representing SPR to excite in cloth（See Fig. 3（b））.SPR produces dipole in the communication process of metal surface with nano gap
Vibration, the surface field that SPR is produced obtains enhanced Raman letter with nano slit dipole collective effect enhancing surface local electric field
Number.
Specific detection includes：Illumination white light（It is not drawn into）By the focal imaging of lens 4 in receiving screen after the light splitting of Amici prism 3
On 5, general imaging inspection is carried out, including sample focusing, regional choice and the observation of sample profile etc..Human eye 1 can be poly- by lens 2
The burnt picture on focal plane directly observes imaging.Two kinds of laser of wavelength 15 are introduced outside simple microscope white-light illuminating light path
（Same light path system for example is applied from two ports, 633 nanometers and 785 nanometer lasers are introduced respectively）, through one group of lens 14
With 12 one-tenth directional lights, wherein using position phase diffuser 13 to remove the spatial coherence of light to eliminate laser imaging image patch（SPR is imaged
With）, position photo 11, polarizer 10 are used to modulating polarization state.Parallel laser light beam imports microcobjective 8 through Amici prism 7, gathers
The burnt nano slit array grating SPR-SERS chip areas on sample stage 9.Focus on the light on SPR-SERS chip areas
Line imports next Amici prism 6 by Amici prism 7 again after collecting the reflected beams through microcobjective 8.Collimated light beam is after filtering
Piece 16 respectively enters SPR imaging systems and SERS detecting systems：Excite the route parallel beams of SPR all by filter plate 16 by
Lens 17 are focused on and import CCD camera（Charge Coupled Device, charge-coupled image sensor）Camera 18, is connect by CCD camera
Receive spr signal（Fourier transform plane）Computer shows SPR pictures, and two blanking bars that SPR is excited are represented by measurement（Such as Fig. 3
（b））Sign excites the movement of angle；Raman scattering light is all reflected by filter plate 16 and changes light path by speculum 19
Direction, then after piece 20 focus on Raman detection region by lens 21 after filtering（Including grating 23,24 and CCD camera 25）On 26
Slit 22, receive the signal of Raman spectrum by Raman detection region 26, the intrinsic peak of analyzing molecules is directly differentiating molecule.
In sum, key of the invention is：Surface plasma resonance is excited and surface-enhanced Raman is excited same
Realized on one chip, using microscope imaging, detected while realizing surface plasma with surface-enhanced Raman.By measurement
The mobile judgement of SPR peak positions excites the change of angle, to determine that the caused surface of chip surface biomolecule reaction is effectively rolled over
Penetrate the change of rate.Meanwhile, the Local field enhancement caused by the coupling in metal surface SPR and nanometer gap can be used to measure Raman
Spectrum is directly differentiating biomolecule in itself.SPR measurement and the direct judgements of Raman signal indirectly, is accurately to judge sensitive surface
On biological respinse increased certainty.To sum up, compound micro-imaging technique proposed by the present invention have low cost, high precision,
Advantage that can be practical, can be widely applied to the fields such as biological detection.
Brief description of the drawings
Examples of the present invention will be described by way of reference to the accompanying drawings, wherein：
Fig. 1 is the compound micro-imaging schematic diagram of SPR-SERS dual-mode chips.1 is human eye, and 2,4,12,14,17,21 is lens, 3,
6th, 7 is Amici prism, and 5 is receiving screen, and 8 is microcobjective, and 9 is sample stage, and 10 is polarizer, and 11 is position photo, and 13 is position phase
Diffuser, 15 is laser, and 16,20 is filter plate, and 18,25 is CCD camera, and 19 is speculum, and 22 is slit, and 23,24 is grating,
26 is Raman detection region.
Fig. 2（a）It is the unilateral type schematic diagram of composite core of AFM measurement.Show nanometer gap array structure and receive
Rice gap, the elevation information such as Fig. 2 in figure bend region（b）.
Fig. 2（b）It is the slit scan figure of compound chip.Slit scan figure corresponds to Fig. 2（a）It is oblique in mesoprosopy schematic diagram
The elevation information of line Regional Representative.
Fig. 3（a）It is microscope schematic diagram.It is the angle between objective lens optical axis and actual light,For in cone-shaped beam certain
One ray cast on disk with x-axis direction institute into azimuth, azimuth changes from 0 degree to 360 degree；Coordinate is described
In Fig. 3（b）In.
Fig. 3（b）For incidence angle isWhen SPR pictures.White circular astragal represents the Fourier transform plane of SPR in figure,
Two black blanking bars represent SPR and excite.X-axis direction is along counterclockwise around a circle, correspondence azimuth in figureBecome from 0 degree to 360 degree
Change；In x-axis direction angular aperture is radially corresponded to respectively outward from the center of circleAt from 0 degree to positive and negative maximum aperture angle.
Fig. 4 is SPR excitation-detection platform schematic diagrames.15 is laser, and 27 is 40 times, the microcobjective of numerical aperture 0.65,
28 is lens, and 7 is Amici prism, and 8 is 40 times, the microcobjective that numerical aperture is 0.65 or 100 times, numerical aperture is 0.85,9
It is sample stage, 17 is lens, and 18 is CCD camera, and 29 is computer.
Fig. 5 is the SPR pictures under different dielectrics.Surrounding's medium on metal grating, by air（air）, pure water（H2O）、
1%th, 10%, 25%, 50%, 75% volumetric ratio ethylene glycol is to pure ethylene glycol（Ey_Gl）Change, correspondence represents blanking bar that SPR excites by outer
Lateral center movement.
Fig. 6 is that SPR excites angle with the relation curve of compound change in refraction under different dielectrics.SPR surface dielectrics from
Pure water, 1% ethylene glycol to 100% ethylene glycol change when, excite angle with the variation relation of complex refractive index；Provide 7 data points
And linear fit curve.
Fig. 7 be various concentrations benzenethiol solution under SPR excite angle change curve.Benzenethiol solution is dense in dielectric solution
Degree（Unit is mole M）From 0M, 10-2M、10-1M, 1M, 10M change when, SPR excites angle with the change of benzenethiol solution concentration
Relation；Provide 5 data points and linear fit curve.
Fig. 8 is the Surface Enhanced Raman Scattering Spectrum figure under various concentrations benzenethiol solution.Benzene is corresponding in turn to from top to bottom
Thiophenol concentration is 10-6M、10-5M、10-4M、10-3Four Raman spectrums of M.
Fig. 9 is characterized peak position 1023cm-1Absolute intensity average value with benzenethiol solution concentration variation relation.Dielectric
Benzenethiol solution concentration 10 in solution-6M、10-5M、10-4M、10-3M changes, 1023cm-1Peak position absolute intensity average value is with benzene sulphur
The change of phenol concentration；Provide 4 data points and linear fit curve.
Marked in figure：1 human eye；2 lens；3 Amici prisms；4 lens；5 receiving screens；6 Amici prisms；7 Amici prisms；8
Microcobjective；9 sample stages；10 polarizers；11 position photos；12 lens；13 phase diffuser laser；14 lens；15 laser；16 filters
Wave plate；17 lens；18 CCD cameras；19 speculums；20 filter plates；21 lens；22 slits；23 gratings；24 gratings；25 CCD phases
Machine；26 Raman detection regions；27 40x/0.65 microcobjectives；28 lens；29 computers；Air refers to that ature of coal is air；H2O refers to coal
Matter is pure water；1% refers to that ature of coal is that ethylene glycol is 1 percent than pure water volumetric ratio；10% finger ature of coal is ethylene glycol than pure water volumetric ratio
It is 10；25% refers to that ature of coal is that ethylene glycol is 25 percent than pure water volumetric ratio；50% finger ature of coal is ethylene glycol than pure
Water capacity ratio is 50 percent；75% refers to that ature of coal is that ethylene glycol is 75 percent than pure water volumetric ratio；100% refers to that ature of coal is
Ethylene glycol is 100 percent than pure water volumetric ratio.
Specific embodiment
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine by any way.
Any feature disclosed in this specification, unless specifically stated otherwise, can be equivalent or with similar purpose by other
Alternative features are replaced.I.e. unless specifically stated otherwise, each feature is an example in a series of equivalent or similar characteristics
.
Using compound biological chip shown in Fig. 2：Common optical grating construction forms new grating, new and old grating after being coupled through parameter
Between form nano gap, there are two nano gaps of 10 nanometer scales in a cycle；Compound chip is that screen periods are big
About 400 or 600 nanometers of gap array structure, correspondence SPR excitation wavelengths are respectively 633 or 785 nanometers.For microscope system
System, directional light is focused on through microcobjective, is converged on chip, and the incident light for reaching chip is included determined by zero degree to object lens
Angular aperture, azimuth is all light beams in 0 degree to 360 degree of taper.Incident plane wave is focused into conical distribution by microscope,
Ranges of incidence angles is,It is the maximum aperture angle of incident light wave（Determined by the numerical aperture NA of object lens）,, wherein n is incident medium refractive index.Such as Fig. 3（a）It is shown, incident parallel light be converted into
Firing angle is, the plane of incidence（Correspondence azimuth）It is 0-360 without array light wave.Incident direction is in angular aperture and side
The change of parallactic angle, there is provided go out to represent in the variable scanning required for SPR detections, i.e. angular aperture and azimuth angle distribution
SPR excite such as Fig. 3（b）Shown blanking bar.From microscope schematic diagram, Incident angle distribution is symmetrical, if being entered with side
Firing angle excites SPR ripples, must there is the SPR ripples that an opposite incidence angle excites another backpropagation.
The SPR excitation-detection platforms of compound dual-mode chip are as shown in Figure 4.633nm or 785nm wavelength lasers 15 are through micro- thing
Mirror 27（40x/0.65）And lens 28 filter, collimate after turn into directional light, through Amici prism 7 import microcobjective 8（40x/0.65
Or 100x/0.85）Focus on print（It is placed in sample stage 9）.Light on chip is reflected by object lens 8, light splitting rib
Change direction after mirror 7, focused on by lens 17, spr signal, computer are received with CCD camera 18 on its Fourier transform plane
The image-forming information of 29 record CCD cameras 18.SPR bodies refractive index is carried out in this detection platform to demarcate and the survey of face refractive index sensitivity
Examination.
Carry out the demarcation of SPR bodies refractive index sensitivity：Various concentrations percentage ethylene glycol solution respectively according to ethylene glycol with go
The volume fraction of ionized water is 0%, 1%, 10%, 25%, 50%, 75%, 100% configuration sample；When the test of each concentration point is carried out,
Solution is taken out with pipette, is dropped on chip, surrounding places pad, covers a piece of cover glass to ensure that liquid sample thickness is 100
Micron.From 785 nanometer lasers as excitation source, reflected light is collected by 40x/0.65 microcobjectives, change metal grating
On medium, changed to 100% ethylene glycol by air, pure water, 1% ethylene glycol, obtain the micro- SPR pictures under various concentrations（Fu Li
Blade face, the focal plane of imaging len）, such as Fig. 5；By the relation of volumetric ratio, according to n complex refractive index=water capacity percentageN water+
Ethylene glycol percent volume to volumeN ethylene glycol can calculate the refractive index of mixed solution under 785 nano wave lengths；Linear fit SPR surfaces
Dielectric from pure water, 1% ethylene glycol to 100% ethylene glycol change when, excite angle with variation relation such as Fig. 6 of complex refractive index,
Can be calculated every change unit refractive index（Refractive Index Unit, RIU）69.8 ° of Shi Jifa Angulation changes, are combined
S=69.8 °/the RIU of SPR bodies refractive index sensitivity of chip.
Proved from the test with the Raman marking materials benzenethiol generally used in document to carry out compound chip.
SPR biological detections are to measure the change of the caused surface effective refractive index of metal surface reaction, therefore, reflection SPR detection property
The physical quantity of energy is its surface refractive index sensitivity, i.e. superficial molecular absorption sensitivity.Sulphur atom and metal in benzenethiol
Combine to form monolayer；Surface density on metal with reference to monolayer is proportional with the concentration of benzenethiol solution.With shifting
Liquid device takes benzenethiol solution and obtains solubility and be respectively 10 in straight alcohol is analyzed-6M、10-5M、10-4M、10-3M、10-2M、10-1M、1M
Benzenethiol dilution, be placed in supersonic cleaning machine with 60% power normal temperature ultrasound 2 minutes, allow benzenethiol molecule to be evenly distributed on
In solution.
Before the test of each SPR concentration point is carried out, chip is sequentially placed into respectively and above-mentioned matches somebody with somebody 10-2M、10-1M、1M、10M
In benzenethiol solution, immersion allows its adsorption benzenethiol molecule for 2 hours.Print is taken out from benzenethiol solution after immersion, is put
Enter in ethanol solution taking-up after rinsing 2-3 second.Print after rinsing is put into nitrogen drying cupboard, is surveyed after being dried in nitrogen stream
Examination is standby.Composite test print is taken on sample stage 9, is focused with 100x/0.85 microcobjectives and is excited SPR.According to above-mentioned
Test process determines spr signal of the compound chip when benzenethiol solution is not deposited, used as the reference point that concentration is 0 M.
Before the test of each Raman concentration point is carried out, chip is put into 10-6M、10-5M、10-4M、10-3In M benzenethiol solution
Immersion is taken out after 4 hours, is taken out after being rinsed 2-3 seconds in ethanol solution.Print after rinsing is put into nitrogen drying cupboard,
Test standby after being dried in nitrogen stream.Test sample is put into Raman-atomic force combined system Raman detection platform with tweezers
On sample stage, using 633 nanometers or 785 nm wavelength lasers as excitation source, reach the laser power of sample for 0.1 milliwatt,
2.5 milliwatts.It is focused with 50x/0.5 object lens, it is 2 to excite spot size, scan 3 times, integrate 3 seconds.
Test sample is taken on sample stage 9 shown in Fig. 4, is focused with 100x/0.85 microcobjectives and is excited SPR, selected
633 nanometer lasers are used as excitation source.According to aforementioned sample preparation method, the SPR that print to be measured carries out 0M benzenethiol concentration is answered
Close chip testing；Carry out 10 successively again-2M、10-1M, 1M, 10M benzenethiol strength solution immersion treatment SPR compound chips carry out
Test.
When in dielectric solution benzenethiol solution concentration from 0M, 10-2M、10-1M, 1M, 10M change when, SPR excite angle with
The variation relation of benzenethiol solution concentration is as shown in fig. 7, each data point represents on test sample region the flat of 4 diverse locations
Average, the standard deviation value in measurement is given with error bar；Oblique line is to excite the average value of angle to be fitted with the SPR under each concentration
Linearity curve.When benzenethiol concentration is relatively low, such as 10-2M、10-1M；When benzenethiol concentration is 10M, SPR excites angular deviation
It is larger.According to linear fit equation, often change unit concentration and swash
1.5 ° of Angulation changes of hair, the surface induction sensitivity S of compound chip surface=1.5°/M。
Using compound chip, SPR and Raman scattering detection can be simultaneously obtained, only reflected signal need to be passed through Raman spectrum
Instrument, and spr signal is imported in SPR imagers.The Raman of use-atomic force combined system Raman detection platform is examined with Fig. 1 Ramans
Survey Some principles identical.Very sensitive to high concentration benzenethiol molecule in view of compound chip, excitation intensity reaches saturation, uses
Low Concentration of Benzene thiophenol solution carries out Raman detection.It is focused with 50x/0.5 object lens, focal beam spot size is 2 microns, scanning 3
Secondary, 3 seconds of integration are illustrated in figure 8 10-6M、10-5M、10-4M、10-3Raman spectrogram under M concentration benzenethiol solution.From it is lower to
On be corresponding in turn to 10-6M、10-5M、10-4M、10-3Tetra- Raman spectrums of M, each characteristic peak of benzenethiol is obvious；With benzenethiol concentration
Reduce, Raman light intensity is decreased.When concentration is reduced to 10-15MWhen, can still detect stronger Raman signal.In Raman spectrum
Characteristic peak and signal to noise ratio high, the Raman enhancement effect that display is higher by, the enhancer of SERS is 106。
The absolute intensity average value for taking feature peak position is Fig. 9 with the variation relation of benzenethiol solution concentration.Each data point
Represent test sample region Raman spectrum 1023cm-1The absolute intensity average value of peak position, wherein to each concentration spot scan three times
And average；Oblique line is 1023cm under each concentration-1The linear fit curve of peak position absolute strength value,；Standard deviation is marked with the error line in scheming.When benzenethiol concentration is higher
When, such as 10-4M、10-3M；Deviation is larger due to concentration saturation for absolute intensity.
SPR microscopic imaging devices and commercial Raman microscopic system with reference to shown in Fig. 4, using nano slit array grating knot
Structure, surface plasma SPR detections are realized with SERS SERS detections in same chip.Using Fig. 1 of the present invention
Microscopic system, with reference to large-area nano slit array optical grating construction as SPR-SERS compound chips, you can in same SPR-
On SERS chips, while realizing efficient, the high-sensitivity detection of SPR and Raman.
The invention is not limited in foregoing specific embodiment.The present invention is expanded to and any in this manual disclosed
New feature or any new combination, and disclose any new method or process the step of or any new combination.
Claims (5)
1. a kind of composite surface plasma（Surface Plasmon Resonance, SPR）Markless detection and surface increase
Strong Raman scattering（Surface-enhanced Raman Scattering, SERS）Micro-imaging technique；
Characterized in that, on the basis of the imaging of simple microscope profile, surface plasma body resonant vibration is realized in same chip
Efficiently excite the enhancing with surface local fields；It is by a kind of large area cycle efficiently to be excited while wherein SPR and SERS
Property nanometer gap array structure realize.
2. micro-imaging technique according to claim 1, it is characterised in that microscope system is by directional light through micro- thing
Mirror is focused on, and is converged on chip, and the incident light for reaching chip includes the angular aperture determined by zero degree to object lens, and azimuth is
Zero degree is to all light beams in 360 ° of taper.
3. efficiently exciting for surface plasma body resonant vibration is realized according to claim 1, it is characterised in that large area is periodically
Nanometer gap array structure can excite SPR, and SPR and nano gap intercouple strengthens surface local fields, enhanced Local field enhancement
Strengthen SPR again, obtain efficiently exciting for SPR；Micro- SPR pictures pass through CCD (Charge Coupled Device, charge-coupled device
Part) camera receives on lens Fourier transform plane.
4. the method for realizing SERS according to claim 1, it is characterised in that large area periodic nanometer
Gap array structure can excite efficient SPR, SPR to produce dipole with nano gap in the communication process of metal surface；SPR
The surface field enhancing of generation obtains strong Raman signal with nano slit local dipole field collective effect enhancing surface local fields.
5. the enhancing efficiently excited with surface local fields of surface plasma body resonant vibration is realized simultaneously according to claim 1,
It is characterized in that two kinds of wavelength are being applied, using same chip, while measuring the spr signal and sign for characterizing angular spectrum information
The Raman signal of strength information.
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