CN115825014A - Surface interface in-situ spectrum and imaging test system coupled by plasmon - Google Patents
Surface interface in-situ spectrum and imaging test system coupled by plasmon Download PDFInfo
- Publication number
- CN115825014A CN115825014A CN202211596445.1A CN202211596445A CN115825014A CN 115825014 A CN115825014 A CN 115825014A CN 202211596445 A CN202211596445 A CN 202211596445A CN 115825014 A CN115825014 A CN 115825014A
- Authority
- CN
- China
- Prior art keywords
- optical
- angle
- imaging
- arm
- optical fiber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention provides a plasmon coupling surface interface in-situ spectrum and imaging test system, which records reflection light intensity or spectrum while changing a laser incidence angle, excites surface plasmon polaritons of a silver film in an optical near-field range in a prism coupling mode when the incident laser angle is just equal to the incident laser angle, observes that the intensity of reflected light reaches the lowest point, namely surface plasmon resonance, keeps incident light exciting a sample at a resonance angle, and then a target molecule system on the surface of the silver film can scatter and absorb electromagnetic surface waves bound in the near-field range and re-emits optical signals to a far field. The system realizes the in-situ combination of technologies such as surface plasmon resonance, surface enhanced Raman scattering spectrum, total internal reflection fluorescence spectrum and imaging by collecting the optical signals.
Description
Technical Field
The invention belongs to the field of surface interface spectral analysis, and particularly relates to a plasmon-coupled surface interface in-situ spectrum and imaging test system.
Background
The research and understanding of the surface interface physical and chemical processes are of great significance to the promotion of many discipline fields including photoelectrocatalysis, sensing detection, integrated optoelectronic devices and the like. Various technical means are established in the scientific exploration process of the surface interface, such as an electron microscope, a scanning probe microscope, a photoelectron spectrum, an electrochemical technology and the like, which provide information about the surface interface at different levels. Spectroscopy, as an important research means, has the advantages of low damage, in-situ, loose operation environment, structural information of reaction substances and the like, and is widely and deeply developed in surface interface science.
Raman spectrum and fluorescence spectrum are two spectrum techniques which are most applied in surface interface analysis, the Raman spectrum is used as a fingerprint spectrum and is related to the vibration mode of molecules in a chemical environment, and the structure and orientation change of the surface interface molecules can be obtained by analyzing information such as peak position, peak intensity, half-peak width and the like of the Raman spectrum. And with the development of the surface enhanced Raman spectroscopy technology, the sensitivity of Raman spectroscopy is remarkably improved, and Raman analysis and detection at a single molecular level are realized by constructing a proper Raman enhanced substrate and selecting an efficient excitation and collection mode. Fluorescence spectroscopy and imaging are highly universal techniques in the study of surface-interface physicochemical processes, and on one hand, fluorescence spectroscopy reflects excited state information of substances, indirect evidence of intermolecular interaction can be obtained by analyzing fluorescence spectroscopy, and on the other hand, a fluorescence labeling method can be used for highly sensitive detection and tracking of surface-interface physicochemical processes, such as obtaining the distribution of target molecules in space, detecting binding events between molecules and a substrate, and the like.
Firstly, the research on the surface interface is concerned about the area in the micro-nano scale range near the interface, and in order to shield the interference from signals or background noise outside the interface, the selected technology should have higher spatial resolution in the direction vertical to the interface. On the other hand, since the analysis object in the surface interface problem is often a thin film composed of a single layer or a few layers of molecules, the analysis technique must have a sensitivity high enough to realize the collection of a signal of a target substance at a low concentration. The physicochemical processes that finally occur at the surface interface are highly complex, and in order to resolve such problems, the use of multiple analytical techniques in combination is required, thereby achieving the characterization of the target in different dimensions.
Surface plasmons provide an effective solution to overcome the above problems, and first, the electromagnetic field has a small penetration depth in the direction perpendicular to the metal-dielectric interface, thereby effectively limiting the spectral detection range to the interface region. On the other hand, due to the light field regulation and control capability of the plasmons, the high concentration of the excitation field energy in a hot spot region can be realized, and the electromagnetic field effect can remarkably enhance the intensity of Raman and fluorescence spectrum signals.
In the prior art, patent CN1657914A and academic articles (Liu, yu, et al review of scientific instruments 81.3 (2010): 036105.) establish a SPR-SERS in-situ testing device in the two works, and the difference of the invention is three aspects, 1, a prism reflection side is used for collecting Raman signals instead of an air transmission side, so that the directional emission effect of the plasmon is effectively utilized. 2. A polarization beam splitter prism is added to the reflecting arm, so that optical signals of S polarization and P polarization can be measured simultaneously, and the method can be applied to research related to interface molecular orientation. 3. The invention can be applied to testing SPR and SERS, and can also carry out total internal reflection fluorescence spectrum and imaging test, thereby realizing the cooperative use of the three technologies of SPR-SERS-TIRF. 4. In the invention, the excitation light is coupled into the system through the optical fiber, rather than directly fixing the laser on the incident arm, so that the excitation wavelength can be conveniently switched.
Disclosure of Invention
In order to solve the problems in the surface plasmon resonance, surface enhanced Raman spectroscopy, total internal reflection fluorescence microscopic imaging and other technologies, the invention constructs an incident angle-regulated plasmon coupling surface fluorescence and Raman spectroscopy in-situ test platform, which records the reflected light intensity or spectrum while changing the laser incident angle, and when the incident laser angle just excites the surface plasmon polariton of the silver film (or gold film) in the optical near-field range in a prism coupling mode, the observed reflected light intensity reaches the lowest point, which is called surface plasmon resonance. Keeping incident light to excite a sample at a resonance angle, scattering and absorbing electromagnetic surface waves bound in a near-field range by a target molecule system modified on the surface of the silver film, and re-transmitting an optical signal to a far field. The device realizes the in-situ combination of the technologies of surface plasmon resonance, surface enhanced Raman scattering spectrum, total internal reflection fluorescence spectrum, imaging and the like by collecting the optical signals.
The invention relates to a plasmon-coupled surface interface in-situ spectrum and imaging test system, which comprises a laser coaxial adjusting and optical fiber coupling system, a rack and rotating arm driving device, a variable-angle incident arm and optical excitation system, a variable-angle reflecting arm and optical signal acquisition system, a sample stage, a prism mounting frame and a micro-spectrum acquisition and imaging system, wherein the rack and rotating arm driving device is connected with the laser coaxial adjusting and optical fiber coupling system; cylindrical prisms are arranged on the sample stage and the prism mounting rack;
the laser coaxial adjustment and fiber coupling system comprises: the long-pass dichroic mirror combines a group of lasers with different wavelengths into one beam, the beams are reflected by the reflecting mirror and then coupled by the optical fiber coupler into an optical fiber, and finally the optical fiber is transmitted to the variable-angle incident arm and the optical excitation system;
the variable angle incident arm and optical excitation system includes: the device comprises an optical fiber flange, an aspheric lens, a linear polarizer, a half-wave plate, an adjustable diaphragm and a focusing objective lens; the light beam coupled to the free space by the optical fiber is firstly collimated by the aspheric lens, then linearly polarized light with adjustable polarization direction is generated by the linear polarizer and the broadband half-wave plate in sequence, the linearly polarized light is subjected to spatial filtering by the adjustable diaphragm, and finally is focused by the focusing objective lens to enter the cylindrical prism;
the variable angle reflection arm and optical signal acquisition system comprises: the device comprises a collecting objective lens, an optical filter, a first focusing lens, a first optical fiber connector, a polarization beam splitter prism, a second focusing lens and a second optical fiber connector; the light reflected by the cylindrical prism passes through the light filter after being collected by the collecting objective lens and enters the polarization beam splitter prism, the S polarized light is reflected, the P polarized light passes through the polarization beam splitter prism, and the reflected light and the transmitted light are focused to the photodiodes or the centers of the optical fibers fixed at the first optical fiber joint and the second optical fiber joint through the first focusing lens and the second focusing lens respectively;
the micro-spectrum acquisition and imaging system comprises an assembly imaging objective lens, a reflector, a long-pass filter, a third focusing lens, a camera, a 1:1 beam splitting sheet, a fourth focusing lens and a spectrometer; the imaging objective lens collects Raman and fluorescence signals of a sample at an interface, the optical signals are firstly reflected by the reflecting mirror and then filtered by the long-pass optical filter to remove exciting light, then the optical signals are divided into a reflecting part and a transmitting part by the 1:1 beam splitting piece, the reflecting light is imaged on an image surface of the camera through the third focusing lens, and the transmitting light is focused to a slit of the spectrometer through the fourth focusing lens to carry out spectrum collection;
the variable-angle incident arm and optical excitation system, the variable-angle reflecting arm and optical signal acquisition system are symmetrically distributed on two sides of the sample stage and the prism mounting frame, the surface plasmon resonance angle is scanned, and the Raman scattering and fluorescence spectrum are measured at the resonance angle;
the microscopic imaging device is positioned at the bottom of the sample stage and the prism mounting rack and is used for total internal reflection fluorescence imaging and spectrum acquisition.
Further, fluorescence and raman spectrum signals collected by the micro-spectrum collection and imaging system are excited by surface plasmon polaritons of the metal film, and the signals are completely from a sample at the surface interface of the metal film.
Further, fluorescence and raman spectrum signals of the molecules are excited using surface plasmon polaritons of the metal film, the penetration depth of which is adjusted by the rotary variable angle incidence arm and the optical excitation system.
Further, the spectral signals are collected by the variable angle reflecting arm on the side of the cylindrical prism and the optical signal collection system or the microscopic spectral collection and imaging system on the air side.
Furthermore, the variable-angle reflecting arm and the polarization beam splitter prism in the optical signal acquisition system simultaneously measure the intensity of the reflected light or the Raman spectrum in two orthogonal polarization states.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive labor.
FIG. 1 is a schematic diagram of a laser coaxial tuning and fiber coupling system;
FIG. 2 is a schematic diagram of a plasmon-coupled surface interface in-situ spectroscopy and imaging test system of the present invention;
FIG. 3 is a total internal reflection fluorescence image acquired at critical angle excitation;
FIG. 4 is a total internal reflection fluorescence spectrum collected upon critical angle excitation;
FIG. 5 is a SPR reflectance spectrum of a silver film taken on an angular scale and on a normalized light intensity scale;
FIG. 6 is a Raman spectrum of 4-MBA probe molecules collected at the SPR resonance angle;
wherein, 1, a frame and a rotating arm driving device; a variable angle incident arm and optical excitation system, comprising: 2-1 optical fiber flange, 2-2 aspheric lens, 2-3 linear polaroid, 2-4 half-wave plate, 2-5 adjustable diaphragm, 2-6 focusing objective 3 variable angle reflection arm and optical signal collection system, including: 3-1 collecting objective lens, 3-2 optical filter, 3-3 polarization beam splitter prism, 3-4 first focusing lens, 3-5 first optical fiber interface, 3-6 second focusing lens and 3-7 second optical fiber interface; 4, a sample table and a prism mounting rack; the system comprises a component 5-1, an imaging objective lens, a 5-2 reflecting mirror, a 5-3 long-pass filter, a 5-4 third focusing lens, a 5-5 camera, a 5-6 beam splitting sheet, a 5-7 fourth focusing lens and a 5-8 spectrometer, wherein the component comprises the following components.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without making any creative effort belong to the protection scope of the present application.
In the drawings of the embodiments of the present invention, in order to better and more clearly describe the working principle of each element in the system, the connection relationship of each part in the apparatus is shown, only the relative position relationship between each element is clearly distinguished, and the restriction on the signal transmission direction, the connection sequence, and the size, the dimension, and the shape of each part structure in the element or structure cannot be formed.
The device mainly comprises a laser coaxial adjusting and optical fiber coupling system shown in figure 1, and a plasmon coupled surface interface in-situ spectrum and imaging test system schematic diagram shown in figure 2, and comprises a frame 1 and a rotating arm driving device; 2, a variable angle incident arm and a light excitation system; 3, a reflecting arm with a variable angle and an optical signal acquisition system; 4, a sample table and a prism mounting frame; 5 micro-spectral acquisition and imaging system.
As shown in fig. 1, a group of lasers with different wavelengths are combined into one beam by a long-pass dichroic mirror, and the beam is reflected by a reflector, coupled by an optical fiber coupler, enters an optical fiber, and finally transmitted to a light path of an incident arm. The wavelengths of the lasers used in the invention can be 375nm,405nm,473nm,532nm,633nm and the like. The specific wavelength of the laser may be switched as desired.
As shown in fig. 2, the optical excitation system and the optical signal collection system are respectively fixed on the variable angle incident arm and the variable angle reflecting arm of the frame and the rotating arm driving device, and can move along the direction perpendicular to the rotating arm through the displacement table to realize the collimation adjustment of the initial optical path. The bottom microscopic imaging and spectrum collecting device is fixed on a horizontal plane slide rail, and the whole device can move independently on an X-Y plane relative to the rotating platform, so that the superposition of a collecting range and an excitation area is realized.
The specific working principle of the invention is as follows: as shown in fig. 1, laser beams emitted by a group of lasers with different wavelengths are combined by a dichroic mirror and then coupled into an optical fiber, the output end of the optical fiber is connected to an optical fiber flange 2-1 of an optical excitation system shown in fig. 2, a light beam coupled to a free space from the optical fiber is collimated by an aspheric lens 2-2, then linearly polarized light with adjustable polarization direction is generated by a linear polarizer 2-3 and a broadband half-wave plate 2-4 in sequence, the linearly polarized light is spatially filtered by an adjustable diaphragm 2-5, and finally focused by a focusing objective lens 2-6 to enter a cylindrical prism with the center coinciding with a rotating shaft (when the laser power density is enough, the laser beam can not be focused, and at the moment, 2-6 is removed).
After the laser is totally internally reflected on the lower surface of the cylindrical prism fixed at the sample stage and the prism mounting frame 4, a surface electromagnetic mode called evanescent field is formed, and the surface electromagnetic wave penetrates through the metal film and excites surface plasmon polaritons at an interface formed by the metal film and air. The detection light path of the optical signal is divided into two paths:
one of the paths of light signals is fixed on the variable-angle reflecting arm on the right side of the figure 1 and the light signal collecting system 3 and is used for collecting reflected light and Raman scattered light, the light signals are firstly collected by the collecting objective lens 3-1 and then pass through the detachable optical filter 3-2 to enter the polarization beam splitter prism 3-3,S, the polarized light is reflected by the polarization beam splitter prism 3-3, and the P polarized light penetrates through the polarization beam splitter prism. The reflected light and the transmitted light are focused through the first focusing lens 3-4 and the second focusing lens 3-6 to the center of the optical detector or the optical fiber fixed at the first optical fiber interface 3-5 and the second optical fiber interface 3-7, respectively. When the system is used for collecting the intensity of reflected light, a photoelectric detector is arranged at an optical fiber interface, and a current signal sequentially passes through a transimpedance amplifier, a low-pass filter and a data collection card and then is transmitted to a computer. When the device works in a Raman spectrum acquisition mode, the optical fiber is arranged at the collection end, and Raman scattering signals enter the spectrometers 5-8 through the optical fiber coupling.
The other path is arranged on the air side below the prism, fluorescence of an interface sample is collected through an imaging objective lens 5-1, a fluorescence signal is firstly reflected through a reflector 5-2, then excitation light is filtered through a long-pass optical filter 5-3, then the fluorescence signal is equally divided into a reflection part and a transmission part by a 1:1 beam splitting sheet 5-6, the reflection light is imaged on an image surface of a camera 5-5 through a third focusing lens 5-4, and the transmission light is focused to a slit of a spectrometer 5-8 through a fourth focusing lens 5-7 for spectrum collection.
In example 1, fluorescence spectroscopy and imaging of silica fluorescent microspheres coated with RBITC (rhodamine B isocyanate).
The laser wavelength used in this example was 532nm (chosen according to the excitation spectral characteristics of the fluorophore).
And (3) performing spin coating on a BK7 glass sputtered with a 45nm silver film and having the size of 10mm x 1mm with an ethanol solution of silica microspheres wrapped with a fluorescent dye RBITC, assembling a sample piece to the bottom of the columnar prism after the spin coating is completed, and matching the refractive indexes of the substrate and the columnar prism through cedar oil.
The photodetector is connected to a receiving end of a variable-angle reflection arm signal acquisition system, such as a polarization beam splitter prism 3-5 or an optical fiber coupler or a photodiode 3-7, the variable-angle incidence arm and optical excitation system 2 and the variable-angle reflection arm and optical signal acquisition system 3 are scanned at the same speed in opposite directions, the direction of the reflection arm is guaranteed to be always kept at a reflection angle, when a minimum value appears in a reflection intensity curve, surface plasmon resonance is realized, at the moment, fluorescence imaging is carried out through a light path at the bottom of the prism to obtain a graph 3, and meanwhile, a fluorescence spectrum is acquired to obtain a graph 4. The bright part in fig. 3 is a silica bead containing a fluorescent dye, because the fluorescent dye is excited in the experiment by an evanescent field confined on the surface of the silver film, so that the image shows good contrast without interference of the fluorescent background. The peak at 560 to 600nm shown in the fluorescence spectrum in FIG. 4 is the fluorescence emission peak of the dye RBITC.
Example 2: the surface plasma resonance enhanced Raman scattering spectrum of the silver film surface modified 4-mercaptobenzoic acid.
The laser wavelength used in this example was 532nm with an optical power density of 10mW/cm 2 。
The Raman spectrum signal collected in this example is from the Raman probe molecule 4-mercaptobenzoic acid.
Firstly, a chromium layer with the thickness of 5nm is magnetically sputtered on BK7 glass with the size of 10mm × 1mm for enhancing the adhesion capability of the silver film to the substrate, and then, the surface of the chromium layer is continuously magnetically sputtered with a silver layer with the thickness of 50 nm. The obtained BK 7/chromium/silver substrate is soaked in an ethanol solution of 4-mercaptobenzoic acid with the concentration of 10uM/L to modify Raman probe molecules of 4-mercaptobenzoic acid on the surface of a silver film. Finally, the obtained BK 7/chromium/silver/4-MBA is attached to a columnar prism, and cedar oil is coated between the substrate and the columnar prism to match the refractive index.
The photoelectric detector is connected to a receiving end of a variable-angle reflection arm signal acquisition system, such as a polarization beam splitter prism 3-5 or an optical fiber coupler or a photodiode 3-7, computer control software is used for driving the variable-angle incidence arm to rotate so as to change the laser incidence angle, meanwhile, the variable-angle reflection arm rotates along with the incidence arm, the reflection light intensity is recorded at the reflection angle, an SPR reflection spectrum as shown in figure 5 can be obtained, the reflectivity is minimum at 44.3 degrees, the energy of the incident light is coupled into surface plasmon polariton of a silver film, and the electromagnetic field mode bound on the surface of the silver film can be further scattered to a far field by molecules to be detected.
Scanning the variable-angle incident arm to the SPR resonance angle, replacing the photoelectric detector on the first optical fiber interface 3-5 or the second optical fiber interface 3-7 with an optical fiber, and connecting the output end of the optical fiber to the spectrometer. The results shown in FIG. 6 can be obtained by collecting the Raman spectrum, and the peaks at the Raman shifts of 1150 and 1600 wave numbers belong to the characteristic peak of 4-mercaptobenzoic acid of the Raman probe molecule.
In the above embodiments, the implementation may be wholly or partially realized by software, hardware, firmware, or any combination thereof. When implemented in software, may be implemented in whole or in part in the form of a computer program product. The computer program product includes one or more computer instructions. When loaded and executed on a computer, cause the processes or functions described in accordance with the embodiments of the application to occur, in whole or in part. The computer may be a general purpose computer, a special purpose computer, a network of computers, or other programmable device. The computer instructions may be stored in or transmitted over a computer-readable storage medium. The computer-readable storage medium can be any available medium that can be accessed by a computer or a data storage device, such as a server, a data center, etc., that incorporates one or more of the available media. The usable medium may be a magnetic medium (e.g., floppy disk, hard disk, magnetic tape), an optical medium (e.g., DVD), or a semiconductor medium (e.g., solid State Disk (SSD)), among others.
While the invention has been described with reference to specific embodiments, the scope of the invention is not limited thereto, and those skilled in the art can easily conceive various equivalent modifications or substitutions within the technical scope of the invention. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
Claims (5)
1. A plasmon-coupled surface interface in-situ spectrum and imaging test system is characterized by comprising a laser coaxial adjusting and optical fiber coupling system, a rack and rotating arm driving device, a variable-angle incident arm and optical excitation system, a variable-angle reflecting arm and optical signal acquisition system, a sample stage and prism mounting rack and a microspectrum acquisition and imaging system; cylindrical prisms are arranged on the sample stage and the prism mounting rack;
the laser coaxial adjustment and fiber coupling system comprises: the long-pass dichroic mirror combines a group of lasers with different wavelengths into one beam, the beams are reflected by the reflecting mirror and then coupled by the optical fiber coupler into an optical fiber, and finally the optical fiber is transmitted to the variable-angle incident arm and the optical excitation system;
the variable angle incident arm and optical excitation system includes: the device comprises an optical fiber flange, an aspheric lens, a linear polarizer, a half-wave plate, an adjustable diaphragm and a focusing objective lens; the light beam coupled to the free space by the optical fiber is firstly collimated by the aspheric lens, then linearly polarized light with adjustable polarization direction is generated by the linear polarizer and the broadband half-wave plate in sequence, the linearly polarized light is subjected to spatial filtering by the adjustable diaphragm, and finally is focused by the focusing objective lens to enter the cylindrical prism;
the variable angle reflection arm and optical signal acquisition system comprises: the device comprises a collecting objective lens, an optical filter, a first focusing lens, a first optical fiber connector, a polarization beam splitter prism, a second focusing lens and a second optical fiber connector; the light reflected by the cylindrical prism passes through the light filter after being collected by the collecting objective lens and enters the polarization beam splitter prism, the S polarized light is reflected, the P polarized light penetrates through the polarization beam splitter prism, and the reflected light and the transmitted light are focused to the centers of the photodiodes or the optical fibers fixed at the first optical fiber joint and the second optical fiber joint through the first focusing lens and the second focusing lens respectively;
the micro-spectrum acquisition and imaging system comprises an assembly imaging objective lens, a reflector, a long-pass filter, a third focusing lens, a camera, a 1:1 beam splitting sheet, a fourth focusing lens and a spectrometer; the imaging objective lens collects Raman and fluorescence signals of a sample at an interface, the optical signals are firstly reflected by the reflecting mirror and then filtered by the long-pass optical filter to remove exciting light, then the optical signals are divided into a reflecting part and a transmitting part by the 1:1 beam splitting piece, the reflecting light is imaged on an image surface of the camera through the third focusing lens, and the transmitting light is focused to a slit of the spectrometer through the fourth focusing lens to carry out spectrum collection;
the variable-angle incident arm and optical excitation system, the variable-angle reflecting arm and optical signal acquisition system are symmetrically distributed on two sides of the sample stage and the prism mounting frame, the surface plasmon resonance angle is scanned, and Raman scattering and fluorescence spectra are measured at the resonance angle;
the microscopic imaging device is positioned at the bottom of the sample stage and the prism mounting rack and is used for total internal reflection fluorescence imaging and spectrum acquisition.
2. The plasmon-coupled surface-interface in situ spectroscopy and imaging test system of claim 1 wherein fluorescence and raman spectral signals collected by said microscopy spectral collection and imaging system are excited by surface plasmon polaritons of the metal film, the signals being derived entirely from the sample at the surface interface of the metal film.
3. The plasmon-coupled surface-interface in situ spectroscopy and imaging test system of claim 2 wherein surface plasmon polaritons of the metal film are used to excite fluorescence and raman spectroscopic signals of the molecule, the penetration depth of said surface plasmon polaritons being adjusted by said rotating variable angle entrance arm and the optical excitation system.
4. The plasmon-coupled surface in situ spectroscopy and imaging test system of claim 1 wherein the spectral signals are collected by said variable angle reflecting arm on the cylindrical prism side at a variable angle and an optical signal collection system or an air side micro-spectroscopy collection and imaging system.
5. The plasmon-coupled surface-interface in-situ spectroscopy and imaging test system of claim 1, wherein the variable-angle reflecting arm and the polarizing beam splitter prism in the optical signal collection system simultaneously measure the reflected light intensity or raman spectra in two orthogonal polarization states.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211596445.1A CN115825014A (en) | 2022-12-12 | 2022-12-12 | Surface interface in-situ spectrum and imaging test system coupled by plasmon |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211596445.1A CN115825014A (en) | 2022-12-12 | 2022-12-12 | Surface interface in-situ spectrum and imaging test system coupled by plasmon |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115825014A true CN115825014A (en) | 2023-03-21 |
Family
ID=85546811
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211596445.1A Pending CN115825014A (en) | 2022-12-12 | 2022-12-12 | Surface interface in-situ spectrum and imaging test system coupled by plasmon |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115825014A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117969457A (en) * | 2024-03-29 | 2024-05-03 | 中国科学院生态环境研究中心 | Double-color evanescent field scattering imaging device and method for detecting lead ion concentration in water sample |
| CN118169044A (en) * | 2024-05-08 | 2024-06-11 | 北京卓立汉光仪器有限公司 | Microscopic spectrum test system |
-
2022
- 2022-12-12 CN CN202211596445.1A patent/CN115825014A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117969457A (en) * | 2024-03-29 | 2024-05-03 | 中国科学院生态环境研究中心 | Double-color evanescent field scattering imaging device and method for detecting lead ion concentration in water sample |
| CN118169044A (en) * | 2024-05-08 | 2024-06-11 | 北京卓立汉光仪器有限公司 | Microscopic spectrum test system |
| CN118169044B (en) * | 2024-05-08 | 2024-08-06 | 北京卓立汉光仪器有限公司 | Microscopic spectrum test system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN115825014A (en) | Surface interface in-situ spectrum and imaging test system coupled by plasmon | |
| CN103439254B (en) | A kind of point pupil confocal laser Raman spectra test method and device | |
| US5633724A (en) | Evanescent scanning of biochemical array | |
| Al-Zubeidi et al. | Single-particle scattering spectroscopy: fundamentals and applications | |
| USRE44014E1 (en) | Single mode (SM) fiber optical reader system and method for interrogating resonant waveguide-grating sensor(s) | |
| CN106896095B (en) | The micro-imaging technique of composite surface plasma resonance and surface-enhanced Raman | |
| Meyer et al. | Combined SPR and SERS microscopy in the Kretschmann configuration | |
| US5442448A (en) | Device for the laterally resolved investigation of a laterally heterogeneous ultrathin object layer | |
| JP3303100B2 (en) | Optical sensor | |
| EP0677734B1 (en) | Detection of a target substance in a sample | |
| CN110398479B (en) | Micro-spectrum measuring device and method based on optical chip substrate | |
| US20020003627A1 (en) | Doubly-differential interferometer and method for evanescent wave surface detection | |
| CN1312476C (en) | Method and system for detecting biological chip by space phase modulation interference array | |
| CN103344620B (en) | Double mode surface plasmon-coupled emission fluorescence imaging detection device and method | |
| US9535005B2 (en) | Electro-optic grating-coupled surface plasmon resonance (EOSPR) | |
| CN102654457B (en) | Refractive index sensor and detection method thereof | |
| Anderson et al. | Effect of experimental parameters on wavefront-shaping-assisted bidirectional focusing in opaque media | |
| CN107356560B (en) | Total reflection type oblique incident light reflection difference scanning imaging device and using method thereof | |
| US6784999B1 (en) | Surface plasmon resonance detection with high angular resolution and fast response time | |
| Wang et al. | Integrated plasmon-enhanced Raman scattering (i PERS) spectroscopy | |
| US8363224B2 (en) | Fringe locking subsystem and methods of making and using the same | |
| CN216771491U (en) | Polarization resolution second harmonic testing device | |
| Liu et al. | Wide-field optical sizing of single nanoparticles with 10 nm accuracy | |
| Saetchnikov et al. | Simultaneous real-time application and direct comparison of optical resonance sensing and fluorescence tagging techniques for biochemical component detection | |
| Heilman et al. | Tip-enhanced near-field optical microscope with side-on and ATR-mode sample excitation for super-resolution Raman imaging of surfaces |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |