CN215833253U - Angle modulation type SPR sensor based on beam deflector and SPR detection equipment - Google Patents
Angle modulation type SPR sensor based on beam deflector and SPR detection equipment Download PDFInfo
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- CN215833253U CN215833253U CN202121091801.5U CN202121091801U CN215833253U CN 215833253 U CN215833253 U CN 215833253U CN 202121091801 U CN202121091801 U CN 202121091801U CN 215833253 U CN215833253 U CN 215833253U
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Abstract
The utility model provides an angle modulation type SPR sensor based on a light beam deflector and SPR detection equipment, which comprise an emitting end, a modulation module, a detection module and a receiving end, wherein the emitting end comprises a light source, the emitting end is used for generating detection light, the detection light is monochromatic wavelength light, the modulation module comprises the light beam deflector, the detection module comprises a rotary table, a detection pool is arranged on the rotary table, a metal layer and a prism are arranged on the detection pool, the receiving end comprises a receiver, and the detection light sequentially passes through the modulation module and the detection module to enter the receiving end. The turntable can adjust the incident angle of the detection light over a wide range by rotating, and the beam deflector can accurately adjust the incident angle over a small range, thereby enabling the beam deflector-based angle modulation-type SPR sensor to improve the accuracy and range of angle modulation.
Description
Technical Field
The utility model relates to the field of sensor research, in particular to an angle modulation type SPR sensor based on a beam deflector and SPR detection equipment.
Background
Surface Plasmon Resonance (SPR) is a physical optical phenomenon, which is an optical technique for characterizing the change of Surface refractive index coefficient, and the SPR technique can be used to observe the Surface phenomena such as the interaction between molecules and the formation of a thin film in real time. Surface Plasmon (SP) refers to an electromagnetic wave that propagates along a surface between a metal and a dielectric. When a metal film of dozens of nanometers exists between the two medium interfaces, the P polarization component of evanescent waves generated by total reflection enters the metal film and interacts with free electrons in the metal film to generate surface plasma, and under the condition that the incident angle or the wavelength is a certain proper value, the surface plasma and the evanescent waves resonate, so that light energy is absorbed, and the energy of reflected light is rapidly reduced.
The SPR detection technique has been widely used in the research fields of life science, biology, pharmacology, analytical chemistry, etc. as a high-precision, label-free, real-time responsive detection means. The SPR detection technology has the characteristics of capability of detecting the interaction between biological molecules in real time, convenience, rapidness, higher resolution than that of the traditional method, no need of marking samples, less sample requirement and the like, is widely applied to the life science fields of proteomics, cell signal conduction, receptor/ligand, antibody/antigen molecule fishing, immune recognition, cancer research, new drug screening and the like, and is used for dynamically monitoring the interaction process of biological molecules such as protein/protein, protein/nucleic acid, new drug molecules/target protein and the like in real time.
The SPR sensor has three coupling modes, namely prism coupling, optical fiber coupling and grating coupling, and the Kretschmann sensor based on the prism coupling mode has the advantages of simple manufacture, convenient use, easy preparation of a sensing chip and the like, so the SPR sensor is widely adopted. The principle of angle modulation is that an angle scanning curve can be obtained by changing the angle between incident light and a normal and measuring the corresponding light intensity under different angles, the angle corresponding to the lowest light intensity point in the angle scanning curve is called as an SPR resonance angle, and the size of the SPR resonance angle is in direct proportion to the change of the surface refractive index coefficient of a measured sample.
The angle modulation type SPR sensor in the prior art generally adopts three modes of a mechanical scanning mode, a galvanometer scanning mode and a light beam converging mode to realize angle modulation. The existing angle modulation mode has the defects of poor modulation precision and small modulation range.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. To this end, the present invention proposes an angle modulation type SPR sensor based on a beam deflector capable of improving the accuracy and range of angle modulation, and an SPR detection apparatus.
The angle modulation type SPR sensor based on the light beam deflector comprises an emitting end, a modulation module, a detection module and a receiving end, wherein the emitting end comprises a light source, the emitting end is used for generating detection light, the detection light is monochromatic wavelength light, the modulation module comprises the light beam deflector, the detection module comprises a rotary table, a detection pool is arranged on the rotary table, a metal layer and a prism are arranged on the detection pool, the receiving end comprises a receiver, and the detection light sequentially passes through the modulation module and the detection module to enter the receiving end.
According to the angle modulation type SPR sensor based on the beam deflector provided by the utility model, at least the following technical effects are achieved: the turntable can adjust the incident angle of the detection light over a wide range by rotating, and the beam deflector can accurately adjust the incident angle over a small range, thereby enabling the beam deflector-based angle modulation-type SPR sensor to improve the accuracy and range of angle modulation.
According to some embodiments of the utility model, the beam deflector employs an acousto-optic deflector.
According to some embodiments of the utility model, the modulation module comprises a focusing lens disposed between the beam deflector and the optical path of the detection module.
According to some embodiments of the utility model, the modulation module comprises a polarizer.
According to some embodiments of the utility model, the polarizing plate is disposed between the focusing lens and the optical path of the beam deflector, and a collimating lens is disposed between the polarizing plate and the optical path of the beam deflector.
According to some embodiments of the utility model, the receiving end comprises a converging lens disposed between the detection module and the optical path of the receiver.
According to some embodiments of the utility model, the receiver employs a single point detector.
According to some embodiments of the utility model, the prism is a semi-cylindrical prism.
According to some embodiments of the utility model, the detection module comprises a sealing ring disposed between the detection cell and the metal layer.
According to the SPR detection apparatus provided by the utility model, the SPR sensor based on the angle modulation type of the beam deflector provided by the utility model is included.
According to the SPR detection device provided by the utility model, at least the following technical effects are achieved: by using the angle modulation type SPR sensor based on the beam deflector, provided by the utility model, the accuracy of the SPR detection range can be improved, and the use effect of the SPR detection equipment can be improved.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is a schematic diagram of a beam deflector based angle modulation SPR sensor provided in accordance with the present invention.
Reference numerals:
the device comprises a light source 11, a beam deflector 21, a collimating lens 22, a polarizing plate 23, a focusing lens 24, a turntable 31, a detection cell 32, a metal layer 33, a prism 34, a receiver 41 and a converging lens 42.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, the meaning of a plurality of means is one or more, the meaning of a plurality of means is two or more, and larger, smaller, larger, etc. are understood as excluding the number, and larger, smaller, inner, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.
In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.
The angle modulation type SPR sensor based on the light beam deflector comprises an emitting end, a modulation module, a detection module and a receiving end, wherein the emitting end comprises a light source 11, the emitting end is used for generating detection light, the detection light is monochromatic wavelength light, the modulation module comprises a light beam deflector 21, the detection module comprises a rotary table 31, a detection pool 32 is arranged on the rotary table 31, a metal layer 33 and a prism 34 are arranged on the detection pool 32, and the receiving end comprises a receiver, and the detection light sequentially passes through the modulation module and the detection module to enter the receiving end.
In use, the turntable 31 is rotated to roughly adjust the incident angle of the detection light to the detection cell 32, then the beam deflector 21 controls the detection light to scan within a certain angle range, so as to obtain an angle scanning curve within the angle range, and the above operations are repeated to obtain an accurate angle scanning curve within a large range.
According to the beam deflector-based angle modulation SPR sensor provided in the present invention, the turntable 31 can coarsely adjust the incident angle of the detection light over a wide range by rotating, and the beam deflector 21 can precisely adjust the incident angle over a small range, so that the beam deflector-based angle modulation SPR sensor can improve the accuracy and range of the angle modulation.
It is understood that the beam deflector 21 is a generic term for a class of instruments capable of adjusting the angle of beam deflection, and in some embodiments, the beam deflector 21 is an acousto-optic deflector. An Acousto-Optic Deflector (AODF) is a device manufactured according to the principle of Acousto-Optic deflection, and can scan a light beam within a certain angle range or precisely control the output light angle, and the deflection angle of the emergent light of the Acousto-Optic Deflector can be changed by changing the radio frequency driving frequency of the Acousto-Optic Deflector.
When the acousto-optic deflector deflects the laser beam, the acousto-optic deflector can realize continuous scanning, random position scanning and other arbitrary scanning modes within the deflection angle range of the device. Depending on the laser parameters and the corresponding acousto-optic deflector, scanning frequencies in excess of 250kHz can be achieved with uniform diffracted laser power at all scanning positions. The acousto-optic deflector has the advantages of ultra-high scanning speed, wide spectral range, high scanning resolution, high luminous flux and the like. Acousto-optic deflectors typically have limited scan angles. The acousto-optic deflector can realize high-precision scanning in a large-angle range by matching with the rotary table 31.
The beam deflector 21 may also employ an electro-optical deflector that deflects detection light by an electro-optical effect, a magneto-optical deflector that deflects detection light by a magneto-optical effect, or the like.
According to some embodiments of the utility model, the modulation module comprises a focusing lens 24, the focusing lens 24 being arranged between the beam deflector 21 and the optical path of the detection module. The focusing lens 24 is used to focus the detection light into the prism 34.
According to some embodiments of the utility model, the modulation module comprises a polarizer 23. The polarizer 23 is used to polarize the detected light, and in some embodiments, the polarizer 23 is disposed between the focusing lens 24 and the optical path of the beam deflector 21, and the collimator lens 22 is disposed between the polarizer 23 and the optical path of the beam deflector 21. The collimator lens 22 can convert the detection light from the beam deflector 21 into collimated light, and since the incident angle range of the polarizing plate 23 of some specifications is narrow, the arrangement of the collimator lens 22 can increase the selection range of the polarizing plate 23.
It will be appreciated that the polarizer 23 may also be arranged at other locations of the modulation module, for example between the beam deflector 21 and the optical path of the light source 11.
According to some embodiments of the present invention, the receiving end comprises a converging lens 42, the converging lens 42 being arranged between the detection module and the optical path of the receiver 41. In some embodiments, receiver 41 employs a single point detector. The converging lens converges the detection light exiting the prism 34 at different exit angles to a single point, so that a scan curve can be drawn by detecting the light intensity at the converging point using a single point detector. The single-point detector is lower in cost than the area detector and the line detector, and can reduce the manufacturing cost of the beam deflector-based angle modulation type SPR sensor.
It will be appreciated that the single point detector may employ a photodiode or other specific structure.
The detection module adopts a Kretschmann sensor structure, a sample is placed between the metal layer 33 and the detection cell 32, detection light is irradiated onto the metal layer 33 through the prism 34, and the surface plasmon resonance phenomenon is excited by changing the incident angle of incident light. The prism 34 may be a semi-cylindrical prism or an isosceles triangular prism, for example, in some embodiments, as shown in fig. 1, the prism 34 is a semi-cylindrical prism, and the focusing lens 24 is matched with the semi-cylindrical prism, so that the detection light can be vertically incident and emergent, and the refraction of the detection light on the surface of the prism 34 can be avoided. In some embodiments, the metal layer 33 has a thickness of 50nm, and the metal layer 33 and the prism 34 are coupled together by a matching fluid, which can fill the gap of the contact surface, thereby improving the accuracy of the beam deflector-based angle modulation SPR sensor. The metal layer 33 can be attached to the surface of the optical glass sheet to facilitate handling due to the thin thickness of the metal layer 33, the refractive index of the optical glass sheet is consistent with that of the prism 34, the optical glass sheet is attached to the prism 34, and the optical glass sheet and the prism 34 are coupled together through the matching fluid. In some embodiments, the detection module includes a sealing ring disposed between the detection cell 32 and the metal layer 33. The metal layer 33 and the detection pool 32 are sealed by a sealing ring, so that the sample can be protected, and the sample leakage can be avoided.
According to the SPR detection apparatus provided by the utility model, the SPR sensor based on the angle modulation type of the beam deflector provided by the utility model is included.
According to the SPR detection apparatus provided by the present invention, by using the beam deflector-based angle modulation-type SPR sensor provided by the present invention, the accuracy of the SPR detection range can be improved, and the use effect of the SPR detection apparatus can be improved.
The beam deflector-based angle modulation-type SPR sensor provided according to the present invention will be described in detail in one specific embodiment with reference to fig. 1. It is to be understood that the following description is illustrative only and is not intended as a specific limitation on the utility model.
The angle modulation type SPR sensor based on the beam deflector comprises an emitting end, a modulation module, a detection module and a receiving end.
The emitting end comprises a light source 11, the light source 11 is a laser, and the laser generates monochromatic wavelength light.
The modulation module includes a beam deflector 21, a collimator lens 22, a polarizer 23, and a focusing lens 24, which are sequentially disposed. The beam deflector 21 employs an acousto-optic deflector.
The detection module comprises a rotary table 31, a detection pool 32 is arranged on the rotary table 31, and a metal layer 33 and a prism 34 are arranged on the detection pool 32. The rotary table 31 is a mechanical rotary table, and the rotary table 31 is connected with a driving motor which can drive the rotary table 31 to rotate. The metal layer 33 is attached to the detection cell 32, and the space between the metal layer 33 and the detection cell 32 is sealed by a seal ring. The thickness of the metal layer 33 is 50nm, the other surface of the metal layer 33 is attached to an optical glass sheet, the refractive index of the optical glass sheet is consistent with that of the prism 34, the optical glass sheet is attached to the prism 34, and the optical glass sheet and the prism 34 are coupled together through matching fluid. The prism 34 is a semi-cylindrical prism.
The receiving end comprises a receiver 41, the receiver 41 adopts a photodiode, and two converging lenses 42 are arranged between the photodiode and the detection module.
The detection light generated by the light source 11 sequentially passes through the beam deflector 21, the collimator lens 22, the polarizing plate 23, and the focusing lens 24 to enter the prism 34, the detection light irradiates the metal layer 33 to generate an SPR reaction, and the detection light exits the prism 34 and enters the photodiode through the condensing lens 42. In use, the turntable 31 is controlled to coarsely adjust the incidence angle of the detection light into the detection cell 32, then the acousto-optic deflector controls the detection light to scan within a certain angle range, so as to obtain an angle scanning curve within the angle range, and the above operations are repeated so as to obtain an accurate angle scanning curve within a large range.
According to the angle modulation type SPR sensor based on the beam deflector, at least the following functions can be realized by adopting the design: the turntable 31 can adjust the incident angle of the detection light over a wide range by rotating, and the beam deflector 21 can accurately adjust the incident angle over a small range, thereby enabling the beam deflector-based angle modulation-type SPR sensor to improve the accuracy and range of angle modulation.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples" or the like mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
While embodiments of the utility model have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the utility model, the scope of which is defined by the claims and their equivalents.
Claims (10)
1. An angle modulation SPR sensor based on a beam deflector, comprising:
the device comprises an emitting end, a light source (11) and a light source, wherein the emitting end is used for generating detection light which is monochromatic wavelength light;
a modulation module comprising a beam deflector (21);
the detection module comprises a rotary table (31), a detection pool (32) is arranged on the rotary table (31), and a metal layer (33) and a prism (34) are arranged on the detection pool (32);
and the receiving end comprises a receiver (41), and the detection light sequentially passes through the modulation module and the detection module to enter the receiving end.
2. The beam deflector-based angle modulation SPR sensor of claim 1, wherein: the beam deflector (21) adopts an acousto-optic deflector.
3. The beam deflector-based angle modulation SPR sensor of claim 1 or 2, wherein: the modulation module comprises a focusing lens (24), the focusing lens (24) being arranged between the beam deflector (21) and the optical path of the detection module.
4. The beam deflector-based angle modulation SPR sensor of claim 3 wherein: the modulation module comprises a polarizer (23).
5. The beam deflector-based angle modulation SPR sensor of claim 4 wherein: the polarizing plate (23) is disposed between the focusing lens (24) and the optical path of the beam deflector (21), and a collimating lens (22) is disposed between the polarizing plate (23) and the optical path of the beam deflector (21).
6. The beam deflector-based angle modulation SPR sensor of claim 1 or 2, wherein: the receiving end comprises a converging lens (42), and the converging lens (42) is arranged between the detection module and the optical path of the receiver (41).
7. The beam deflector-based angle modulation SPR sensor of claim 6 wherein: the receiver (41) employs a single point detector.
8. The beam deflector-based angle modulation SPR sensor of claim 1 or 2, wherein: the prism (34) is a semi-cylindrical prism.
9. The beam deflector-based angle modulation SPR sensor of claim 1, wherein: the detection module comprises a sealing ring, and the sealing ring is arranged between the detection pool (32) and the metal layer (33).
10. An SPR detection apparatus, comprising: the SPR detection apparatus includes a beam deflector-based angle modulation-type SPR sensor according to any one of claims 1 to 9.
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| CN202121091801.5U CN215833253U (en) | 2021-05-17 | 2021-05-17 | Angle modulation type SPR sensor based on beam deflector and SPR detection equipment |
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| CN202121091801.5U CN215833253U (en) | 2021-05-17 | 2021-05-17 | Angle modulation type SPR sensor based on beam deflector and SPR detection equipment |
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