CN106883993A - A kind of culture medium prescription for the Bacillus sphaericus that ferments - Google Patents
A kind of culture medium prescription for the Bacillus sphaericus that ferments Download PDFInfo
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- CN106883993A CN106883993A CN201510931387.7A CN201510931387A CN106883993A CN 106883993 A CN106883993 A CN 106883993A CN 201510931387 A CN201510931387 A CN 201510931387A CN 106883993 A CN106883993 A CN 106883993A
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- culture medium
- bacillus sphaericus
- medium prescription
- ferments
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Abstract
The invention belongs to biomedicine technical field, a kind of culture medium prescription for the Bacillus sphaericus that ferments is specifically disclosed.Culture medium prescription of the present invention is grouped into by each group of following mass percent：Dregs of beans 4 ~ 8%, fish meal 3 ~ 5%, beancake powder 3 ~ 5%, peanut starch 7 ~ 11%；Balance of water.The invention provides a kind of fermentating formula for the Bacillus sphaericus that ferment, relative to traditional zymotic formula, the solid content of this formula is lower, cheaper and potency is higher.
Description
Technical field
The invention belongs to biomedicine technical field, in particular it relates to a kind of culture medium prescription for the Bacillus sphaericus that ferments.
Background technology
Mosquito is arch enemy of man, and especially in summer, bite by mosquitos not only makes one sufferings unbearably, can also propagate various diseases simultaneously, such as dengue fever, encephalitis, malaria etc., if giving treatment to not in time, these diseases often bring huge life, property loss to human society.Traditional mosquito eradication method is mainly applying chemical medicine and kills adult mosquito, such as arprocarb, decis, found in long-term use, these chemicalses are to human body more or less all with toxic, so the release of chemicals, although effect exterminating mosquito gets instant result, but killing also result in other beneficial insects in human body, environment simultaneously, so popular instantly in environmentally friendly theme, chemical mosquitocide is gradually no longer influenced by concern.Instead it is then biological culicide, present biological culicide is arisen primarily in the bacillus such as thuringiensis, and Bacillus sphaericus are also one kind of Bacillus, its brood cell has stronger and specific toxic action to Ability of Culex Pipiens Larvae.In addition to killing rate is high, biological culicide also has the advantage that, biological culicide is directed to young mosquito, mosquito is strangled in young mosquito state, meanwhile, young mosquito is grown in water, and Bacillus sphaericus are to be located away from soil, pollution will not be produced to environment, will not also influence be produced on the fish in dispenser area, insects.
The content of the invention
The present invention is in view of the shortcomings of the prior art, there is provided a kind of culture medium prescription for the Bacillus sphaericus that ferments.
Above-mentioned purpose of the invention is achieved by the following technical programs.
A kind of culture medium prescription for the Bacillus sphaericus that ferments, is grouped into by each group of following mass percent：Dregs of beans 4 ~ 8%, fish meal 3 ~ 5%, beancake powder 3 ~ 5%, peanut starch 7 ~ 11%；Balance of water.
The formula for being conveniently employed in fermentation Bacillus sphaericus is made up of corncob, fish meal, corn flour and starch, the present invention for Bacillus sphaericus fermentation character, the optimization design culture medium prescription.
Preferably, the culture medium prescription is grouped into by each group of following mass percent：Dregs of beans 5%, fish meal 4%, beancake powder 5%, peanut starch 8%；Balance of water.
Compared with prior art, beneficial effect of the present invention is：The invention provides a kind of fermentating formula for the Bacillus sphaericus that ferment, relative to traditional zymotic formula, the solid content of this formula is lower, cheaper and potency is higher.
Specific embodiment
The present invention is described in further details with reference to specific embodiment, but embodiment is not limited in any form to the present invention.Unless stated otherwise, the reagent for using of the invention, method and apparatus are the art conventional reagent, method and apparatus.
Embodiment
1
Detection method：Mosquito biologicall test
With reference to WHO（1985）The method of offer：The overnight running water of 100ml is put into the disposal plastic cup of cumulative volume 150ml（Dechlorination）, each cup is put into 20 larvaes in 3-4 ages, adds a little feed, and 3 repetitions of each concentration set 5-6 concentration gradient and one group of blank per secondary survey.After 26 DEG C process 48h at room temperature, larval mortality is checked, surveyed in different time repetition 2-3 times with all one's life.The LC of mosquito larvae is calculated with the softwares of SPSS 13.050, unit represents with mg/l.
Sample titration：When calculating sample potency, the Bacillus sphaericus standard items SPH-88 that must be produced using Pasteur Institut（The ITU/mg of potency 1500）As control, after raw survey terminates, the LC of zymotic fluid and standard items is calculated using the softwares of SPSS 13.050, and zymotic fluid potency, unit are calculated according to formula： ITU/mg.
Experimental result is as shown in table 1；Wherein sample 1 is conventional formulation, and sample 2 is inventive formulation.
The mosquito bioassay results of table 1
Experiment understands that the potency of conventional formulation tunning is relatively low, only 80 ITU/ml, but solid content is higher, reaches 5%, and after pressing formula improvement of the invention, potency is promoted to 175 ITU/mg, and solid content is reduced to 1%.Advantage of the invention is that not only increasing potency, the solid content of fermentation medium is more reduced, and relatively low solid content can aid in the improvement of formulation in the future.
Claims (2)
1. a kind of culture medium prescription for the Bacillus sphaericus that ferments, it is characterised in that be grouped into by each group of following mass percent：Dregs of beans
4 ~ 8%, fish meal 3 ~ 5%, beancake powder 3 ~ 5%, peanut starch 7 ~ 11%；Balance of water.
2. culture medium prescription according to claim 1, it is characterised in that be grouped into by each group of following mass percent：Dregs of beans 5%, fish meal 4%, beancake powder 5%, peanut starch 8%；Balance of water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510931387.7A CN106883993A (en) | 2015-12-15 | 2015-12-15 | A kind of culture medium prescription for the Bacillus sphaericus that ferments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510931387.7A CN106883993A (en) | 2015-12-15 | 2015-12-15 | A kind of culture medium prescription for the Bacillus sphaericus that ferments |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106883993A true CN106883993A (en) | 2017-06-23 |
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| CN201510931387.7A Pending CN106883993A (en) | 2015-12-15 | 2015-12-15 | A kind of culture medium prescription for the Bacillus sphaericus that ferments |
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Citations (6)
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| CN1491080A (en) * | 2001-02-16 | 2004-04-21 | �Ϻ���ͨ��ѧ | Mixture of bacillus thuringiensis subspecies israelensis and bac illus sphaericus for management of resistance to mosquito larvicides |
| CN101347129A (en) * | 2008-09-01 | 2009-01-21 | 佛山市正典生物技术有限公司 | Bacillus thuringiensis suspending agent for killing mosquito |
| CN101864390A (en) * | 2010-06-13 | 2010-10-20 | 福建农林大学 | Mosquito killing bacillus thuringiensis liquid culture medium |
| CN102154171A (en) * | 2011-01-13 | 2011-08-17 | 河北农业大学 | Bacillus thuringiensis capable of killing mosquito larvae effectively |
| CN102168045A (en) * | 2010-12-24 | 2011-08-31 | 北京科为博生物科技有限公司 | Bacillus subtilis preparation and preparation method thereof |
| CN102907458A (en) * | 2012-10-28 | 2013-02-06 | 郑文定 | Biological mosquitocide and preparation method thereof |
-
2015
- 2015-12-15 CN CN201510931387.7A patent/CN106883993A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1491080A (en) * | 2001-02-16 | 2004-04-21 | �Ϻ���ͨ��ѧ | Mixture of bacillus thuringiensis subspecies israelensis and bac illus sphaericus for management of resistance to mosquito larvicides |
| CN101347129A (en) * | 2008-09-01 | 2009-01-21 | 佛山市正典生物技术有限公司 | Bacillus thuringiensis suspending agent for killing mosquito |
| CN101864390A (en) * | 2010-06-13 | 2010-10-20 | 福建农林大学 | Mosquito killing bacillus thuringiensis liquid culture medium |
| CN102168045A (en) * | 2010-12-24 | 2011-08-31 | 北京科为博生物科技有限公司 | Bacillus subtilis preparation and preparation method thereof |
| CN102154171A (en) * | 2011-01-13 | 2011-08-17 | 河北农业大学 | Bacillus thuringiensis capable of killing mosquito larvae effectively |
| CN102907458A (en) * | 2012-10-28 | 2013-02-06 | 郑文定 | Biological mosquitocide and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| MAGDA A. EL-BENDARY: "Bacillus thuringiensis and Bacillus sphaericus biopesticides production", 《J. BASIC MICROBIOL.》 * |
| 任改新等: "球形芽孢杆菌的发酵试验", 《微生物学通报》 * |
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Application publication date: 20170623 |