CN106880053A - Maize germ enzymolysis compound, preparation method and its purposes as health food - Google Patents
Maize germ enzymolysis compound, preparation method and its purposes as health food Download PDFInfo
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- CN106880053A CN106880053A CN201710033832.7A CN201710033832A CN106880053A CN 106880053 A CN106880053 A CN 106880053A CN 201710033832 A CN201710033832 A CN 201710033832A CN 106880053 A CN106880053 A CN 106880053A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses maize germ enzymolysis compound, preparation method and its purposes as health food, belong to health food development applied technical field.It is to be prepared from by the raw material of following weight portion：Maize germ：20~40 parts；Wheat embryo：10~15 parts；Rice germ：5~10 parts.Crude fibre in maize germ, wheat embryo and rice germ is converted to by dietary fiber, polysaccharide etc. by multi-step biological enzyme resolving tech, corn protein is changed into by activated protein, functional polypeptide, oligopeptides etc., the complete nutritional ingredient remained in grain germ by alkali protease, neutral proteinase and flavor protease etc..During biology enzyme enzymolysis in ultrasonic wave added enzymolysis process, enzymolysis time is shortened, improve hydrolysis result, reduce the usage amount of enzyme, improve the activity of enzymolysis product.
Description
Technical field
The present invention relates to refer in particular to one kind with maize germ as primary raw material, using stepwise discretization, ultrasonic wave added enzymolysis, height
Pressure homogenization prepares the method and its purposes as health food of functional polypeptide, belongs to health food development application technology neck
Domain.
Background technology
Cereal（Wheat, corn, rice）Plumule is the basis of corn growing development, there is high nutritive value.Such as jade
The starting point that rice plumule grows as corn kernel, is lifespring.Though maize germ only accounts for corn kernel weight
10% ~ 15%, but nutritive value is extremely abundant, has concentrated in corn kernel more than 80% fat and inorganic salts, 60% carbohydrate
And 20% high-quality protein, and contain other nutritional ingredients such as phosphatide, sitosterol.Maize germ or a kind of albumen of high-quality
Resource, its protein content is average to be only second to the high-protein plant seed such as soya bean and green soya bean more than 24.3%, and its composition is with white egg
Based on white and globulin, 30% or so is respectively accounted for, glutelin and 5% alcohol soluble protein in addition with 25% or so.Maize germ
Albumen is roughly the same with the amino acid composition in whole-egg protein, must containing human bodies such as abundant leucine, lysine and valines
Need amino acid, AAS（AAS）Higher than oatmeal and polished rice.Compared to other plant albumen, the ammonia of corn germ protein
Base acid constitutes complete and equilibrium, can meet demand of the human body to various essential amino acids.Corn germ protein is except with equal
The amino acid composition of weighing apparatus is outer, also with biological value higher（Biological Value, BV）, it is only second to egg（94%）With
Milk（85%）.The efficiency ratio of defatted corn germ albumen（Protein Efficiency Ratio, PER）About 2.3, with
Casein, soybean protein are close, substantially conform to WHO/FAO recommendations, and BV values are 70.8%, and its digestion coefficient is 80.
Wheat embryo accounts for the 2.5% of wheat seed, the protein of high-quality, fat, the life of various dimensions containing extremely abundant
Element, mineral matter and some not yet clear and definite micro physiologically active components, are described as " the natural treasure-house of the mankind ", " life of the mankind
Source ".Protein content is up to more than 30% in wheat germ, contains 8 kinds of amino acid needed by human, the particularly content of lysine
18.5% is accounted for, amino acid needed for baby's child's growth and development can be supplemented.Wheat germ contains abundant multivitamin, vitamin B1Contain
Amount is 57 times of egg, vitamin B2Content be 2 times of egg, the content of vitamin E is more up to 34.9mg/100g, battalion
Foster scholar claims it to be the treasure-house of natural VE, the effect with enhancing brain cell activity, promotes baby's child's brain development.Wheat germ
Also containing several mineral materials and trace elements such as calcium, potassium, magnesium, iron, zinc, chromium, selenium, phosphorus, manganese, copper, the growth hair to promoting children
Educate and play an important roll, be a kind of splendid natural minerals element source of supply.Glutathione in wheat germ protein is in human body cell
Redox in play an important role, the formation of internal peroxide can be avoided, with protection brain, promote infant growth hair
The function such as educate.Containing abundant phosphatide, choline in wheat germ, with promotion brain development, deepen memory and raising intelligence
Etc. function.
On its plumule, plumule contains rich in protein and other nutritional ingredients to the Major Nutrient of rice.Plumule is one
3% is only accounted for by weight in grain rice, but its nutrition accounts for one the 50% of rice, is described as " nutrient source of being bestowed by heaven ".Plumule contains abundant
Protein and starch, dietary fiber, multivitamin and bioactivator, containing microelements of calcium, iron, zinc, selenium etc..Rice
More than 20%, natural VE is 200 mg/100g-300 mg/100 in lipid for protein and lipid content in plumule
G, aliphatic acid 70% in lipid contain abundant vitamin and mineral matter above is unrighted acid.Rice germ is because contain
Have compared with fattiness, therefore be usually used to produce rice germ oil.Rice germ oil is natural tocopherol level highest oil product, naturally
Due to being coexisted with a small amount of phytosterol, the action effect of its combined state becomes apparent vitamin E.Ammonia in rice germ protein
Base acid composition is more balanced, and wherein essential amino acid ratio meets the nutrition Reasonable Mode of FAO/WHO suggestions.Therefore, rice
Germ protein matter is a kind of good protein for belonging to full price, and its quality can compare favourably with egg.Rice germ is main by clear egg
In vain, globulin, alcohol soluble protein and glutelin composition, various separation albumen have different processing and functional characters.
The patent of the existing processing technology about above-mentioned several grain germs in the country or research, for grinding for maize germ
Study carefully, such as a kind of oil-containing corn embryo albumen powder of patent and preparation method thereof（Application number 200810006483）, will be new in the patent
Fresh maize germ produces the low denaturation oil-containing corn embryo dregs of rice by low temperature drying, low temperature parch, cold pressing；With what is obtained
The oil-containing corn embryo dregs of rice are raw material, and oil-containing corn embryo albumen powder is obtained with the heavy technique of alkali carries acid.As patent one kind can inhale type jade
The processing technology of rice plumule anti-oxidation peptide Guiling Jelly（Application number 201310217224）, being disclosed in the patent can inhale type corn
The preparation method of plumule anti-oxidation peptide Guiling Jelly, including 6~10 parts of maize germ antioxidant peptide powder, 70~85 parts of Guiling Jelly pulvis
Mixing, adds 100~120 parts of frozen water mixing, stirring to pasty state to add 1000~1200 parts of 40 DEG C of warm water, be heated to 90 DEG C, endure
It is sterilized filling to lotion, it is maize germ anti-oxidation peptide Guiling Jelly finished product, wherein maize germ anti-oxidation peptide after cooling encapsulation
Powder is using 2709 alkali proteases, in pH9~11, enzyme-to-substrate concentration ratio 8%~12%, concentration of substrate 2%~4%, enzyme
Solution is digested under conditions of temperature 50 C~60 DEG C to corn germ protein, then the maize obtained through ultrafiltration, freeze-drying
Bud antioxidant peptide powder.
Such as a kind of wheat-germ oil of patent and the combined preparation process of wheat plantule protein（Application number 201110327926）,
The combined preparation process of a kind of wheat-germ oil and wheat plantule protein is disclosed in the patent, with wheat germ powder as raw material,
Using supercritical CO2Fluids extraction extract wheat-germ oil, and with fluid rectification method purify raising wheat embryo grease matter
Amount；The wheat germ protein in the overcritical residue of wheat germ powder, the wheat embryo are extracted using low temperature water extraction, ultra-filtration and separation technique again
Oil and wheat germ protein can be widely applied to medicine, health food." prepared by the joint of wheat plantule protein and its derived product for patent
Method（Application number 200710078256）", with drying wheat plumule as raw material, by degreasing, solvent extraction, separation, enzyme hydrolysis
Etc. technique, prepare anti-oxidant wheat embryo alcohol soluble protein, wheat embryo, anti-senility liquid, hydrolyzed wheat plumule peptide concentrate and
The multiple products such as powder, wheat plantule protein feed addictive, moreover it is possible to which decolorising agent, ethanol or the methyl alcohol to being used during production are molten
The equal reproducible utilization of agent.
For the research of rice germ, a kind of extracting method of rice germ albumen（Application number 201510104665）, it is public
A kind of extracting method of rice germ albumen is opened, has been comprised the following steps：(1) degreasing sample is weighed, after being dissolved in water, in water-bath
Preheated in pot, be put into concussion in constant temperature blender with magnetic force, protein is dissolved in sample liquid, 4000r/min is centrifuged 20 minutes, collects
Supernatant, as albumin；(2) the above-mentioned residue extracted through water is taken, 6% sodium chloride solution is added, fully after dissolving, is pressed
Same method collects supernatant, as globulin；(3) again by the ethanol solution of residue addition 70%, supernatant is collected in dissolving
Liquid, as protein,alcohol-soluble；(4) residue precipitation is added 0.4% NaOH, fully dissolving is in kind obtained paddy
Albumen.
It is above-mentioned to have the following disadvantages in the prior art：
First, processing step is cumbersome, either studies more wheat germ, or studies less rice germ, all using complexity
Technique processed, be substantially first prepare grain germ oil, the grouts being then left with, step by step arithmetic albumen, or
Extract and digest prepare functional polypeptide again after albumen, processing step is complicated, be unsuitable for technology production, and due to substep at
Reason, product yield is low therefore extremely low for the utilization rate of plumule！
Second, fresh grain germ particle reaches the purpose of enzyme dehumidifying of going out by certain treatment, can just be easy to storage.But by
Giving birth to China's processing method in plumule uses the open firing, naked light can well to keep the peculiar taste material of food, but can make
Into environmental pollution, and efficiency of utilization is low；Meanwhile, special messenger being needed using open firing and is managed, low production efficiency is not suitable for
Large-scale production.
3rd, during existing enzymatic isolation method prepares albumen or polypeptide, because the grain germs such as maize germ contain oil
The Multiple components such as fat, starch, cellulose so that its albumen is difficult by protease hydrolyzed, and then cause the degree of hydrolysis of enzymolysis protein
Relatively low, efficiency of pcr product is very low, it is difficult to be processed further utilizing.
The content of the invention
To solve the above problems, the invention is mainly by physical stabilization treatment, color selector screening, ultramicro grinding, many
The technological means such as step enzymolysis, ultrasonic wave added enzymolysis prepare the complex enzyme hydrolysis thing based on maize germ, and study its effect and conduct
The possibility of functional food.
Maize germ complex enzyme hydrolysis thing of the present invention, is prepared from by the raw material of following weight portion：
Maize germ：20~40 parts；
Wheat embryo：10~15 parts；
Rice germ：5~10 parts.
It is preferred that being prepared from by the raw material of following weight portion：
Maize germ：40 parts；
Wheat embryo：15 parts；
Rice germ：10 parts.
Wherein described maize germ enzymolysis compound, is prepared obtains as steps described below：
（1）Plumule stabilization processes；Maize germ, wheat embryo and rice germ are mixed according to aforementioned proportion, takes infrared
Dry, the water content of plumule is less than 3% after treatment；
（2）Color selector is screened；Dried plumule is put into color selector, clearance rate is more than 99%；
（3）Ultramicro grinding：Dried plumule is allowed to broken using air blast ultramicro powder is broken, so as to germ particles be crushed to
10~25 microns of powder；Grinding time is 10~30 minutes；
（4）Ultrasonic wave added cellulase degradation：In mass ratio it is 1:The plumule of crushing and water are mixed into suspension by the ratio of 5-10
Liquid, is heated to 60 DEG C, adjusts pH to 5.5, and 200-240min is digested under the conditions of 60 DEG C using cellulase, and enzyme concentration is according to enzyme
The mass ratio of enzyme activity and substrate be 400-600u/g；Multifrequency mode ultrasound treatment, ultrasonic time and enzyme are carried out while enzymolysis
The solution time is identical；
Wherein described multiple frequency ultrasonic tupe is：Three frequencies or double-frequency synchronous are ultrasonically treated, ultrasonic power density 80 ~ 120
W/L；The s of ultrasonic pulse working time 10；The s of interpulse period 3.
Three frequencies or the ultrasonically treated ultrasonic frequency of double-frequency synchronous are combined as：35/28 kHz, 35/28/40 kHz or
35/40 kHz。
（5）Frequency sweep ultrasonic auxilin enzyme is digested：PH is to 8.5 for regulation, 50 ~ 60 DEG C is cooled to, according still further to 800-2000 u/
G adds Alcalase alkali proteases, and it is ultrasonically treated to carry out double frequency frequency sweep mode rapidly, the min of enzymolysis time 80 ~ 200.
Wherein step（5）Described in double frequency frequency sweep ultrasonic to fix upper and lower plates spacing when working be 8cm, frequency sweep cycle
400s, intermittently than 1:1（Ultrasonic 10s, interval 10s）, every piece of vibration plate power is 600W up and down.
Wherein step（5）Described in double frequency combination of frequency be： 33 kHz/ 68kHz、33kHz/40kHz、68 kHz/
28 kHz；Most preferably good combination of frequency is 33 kHz/68 kHz.
（6）Ultrasonic wave added flavor protease is digested：PH is to 5.5 for regulation, is cooled to 40 DEG C, adds according still further to 600-5000 u/g
Enter flavor protease, adverse current impulse ultrasound assistance enzymolysis, the min of enzymolysis time 120 ~ 300 are carried out rapidly.
The design parameter of adverse current impulse ultrasound assistance enzymolysis is close 1 ~ 5s of pulse width, 1 ~ 5s of pulse spacing, ultrasonic power
80 ~ 100 W/L of degree；Supersonic frequency 28kHz, feed liquid passes through ultrasonic probe in the way of circulated in countercurrent；
（7）After enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 80 DEG C and is kept for 20 minutes, be then cooled to 50 DEG C, and add
It is 1 to enter according to plumule and maltodextrin:1 mass ratio adds maltodextrin to stir, and homogeneous is carried out by high pressure homogenizer
Three times, each pressure is respectively 40 MPas, and 50 MPas, 55 MPas, homogenizing time is respectively 10 min, 15min, 10 min most
After be spray-dried, the process conditions of spray drying are：160 ~ 200 DEG C of intake air temperature, air outlet temperature is 70 ~ 100 DEG C,
Obtain maize germ complex enzyme hydrolysis produce product.
Above-mentioned maize germ digests the application of compound, and maize germ enzymolysis compound has stronger external suppression ACE
Activity, can prepare the food with hypotensive activity, food ingredient or health food.
The beneficial effects of the invention are as follows：
First, the present invention is processed by infrared and microwave, color selector is screened, the corn of ultramicro grinding preparation uniform and smooth
Plumule, wheat embryo and rice germ powder, completely remain the functional component in raw material, meet modern food pursue it is natural,
Nutrition, the overall development trend of health；
Second, the crude fibre in maize germ, wheat embryo and rice germ is converted to by multi-step biological enzyme resolving tech
Dietary fiber, polysaccharide etc., are changed into corn protein by alkali protease, neutral proteinase and flavor protease etc.
Activated protein, functional polypeptide, oligopeptides etc., the complete nutritional ingredient remained in grain germ.
3rd, biology enzyme is applied with multi-mode ultrasonic wave, frequency sweep ultrasonic ripple and counter current ultrasonic wave during digesting, ultrasound
During assistance enzymolysis, enzymolysis time is shortened, improve hydrolysis result, reduce the usage amount of enzyme, improve enzymolysis product
Activity.
Brief description of the drawings
Fig. 1 is multi-mode ultrasonic wave added enzymolysis equipment structure chart, wherein 1,2,3 is ultrasonic vibrating plate, 4 to contain liquid device, and 5 is water
Bath, 6 is temp probe, and 7 is circulating pump, and 8 is Computerized controller, and 9,10,11 is ultrasonic controller.
Fig. 2 is the equipment drawing of frequency sweep double-frequency ultrasound pretreatment unit, 1- ultrasounds pond, 2- thermometers, 3- thermostatted water bathing pools, 4-
Vibration plate on ultrasonic wave, vibration plate under 5- ultrasonic waves, 6- sample treatments region, 7- computer controllers, 8- supersonic generators.
Fig. 3 is adverse current ultrasound processing equipment structural representation, and 1 pops one's head in for energy-gathered ultrasonic, and 2 is ultrasonic wave controller, 3
It is ultrasonic cup type chamber, 4 is charging aperture, and 5 is circulating pump, and 6 to contain liquid device, and 7 is discharging opening.
Specific embodiment
The term for being used in the present invention, unless otherwise specified, typically has those of ordinary skill in the art usual
The implication of understanding.With reference to specific embodiment, and the present invention is described in further detail with reference to data.It should be understood that these
Embodiment is of the invention solely for the purpose of illustration, rather than limits the scope of the present invention by any way.
The enzyme used in the present invention, including：
Alcalase 2.4L FG protease（1.325×105U/g, optimum temperature, 50 DEG C；PH, 8.0）, Novi's letter biotechnology
Co., Ltd；Cellulase：The U/g of enzyme activity 8 000, neutral proteinase（Enzyme activity is 16.0 ten thousand U/g）, flavor protease
（Enzyme activity is 6.9 ten thousand U/g）There is provided by Wuxi enzyme preparation Co., Ltd of Jie Neng sections.
Fig. 1 is the multi-mode ultrasonic wave added enzymolysis equipment that ultrasonic wave added enzymatic isolation method of the invention is used, and the equipment is furnished with one
Platform Computerized controller 8, can set ultrasound works parameter（Ultrasonic power density, frequency, pulse working time, intermittent time
With treatment total time）Control three ultrasonic controllers 9,10,11 respectively, connect respectively three ultrasonic vibrating plates of different frequency 1,2,
3, it is capable of achieving the treatment of single-frequency/two frequency/tri- frequency ultrasonic wave；The aaerosol solution input of enzymolysis will be needed to contain liquid device 4
In carry out the experiment of single-frequency/double frequency/multiple frequency ultrasonic assistance enzymolysis, start circulating pump 7 and enzymolysis liquid be circulated.By water-bath 5
Automatically controlling for enzymolysis liquid temperature is realized with temp probe 6.
Fig. 2 is the equipment drawing of the frequency sweep double-frequency ultrasound pretreatment unit that the present invention is used, and is Jiangsu University's independent development.It is super
Acoustic generator 8 can send the ultrasonic wave for determining frequency and frequency sweep both of which, and separate unit supersonic generator power is 600w.In ultrasonic pond
Placement ultrasonic wave vibration plate upper plate 4 symmetrical above and below and ultrasonic wave lower plate 5, are controlled on ultrasonic wave vibration plate by supersonic generator 8 in 1
Plate 4 and ultrasonic wave lower plate 5；After the setting each parameter of ultrasonic wave of computer controller 7, supersonic generator 8 is controlled, send and meet the requirements
Ultrasonic wave；3 is the thermostatted water bathing pool of equipment in the present invention, by the monitor in real time temperature of thermometer 2, and is adjusted according to need of work
Section medium temperature；Need to liquid raw material to be processed be placed in sample sack to be placed in ultrasonication carried out in treatment fluid region 6.
Fig. 3 is adverse current ultrasound processing equipment of the invention, and the equipment is furnished with ultrasonic wave controller 2, controls ultrasound parameter, gathers
The material that energy formula ultrasonic probe 1 enters ultrasonic cup type chamber 3 to charging aperture 4 is processed, and circulating pump 5 pairs is started while ultrasonic
Feed liquid is circulated, and 6 to contain liquid device, and 7 is discharging opening.
Excessive ACE in human body（ACE）It is one of major reason for causing elevation of the blood pressure.Therefore originally
Invention evaluates its hypotensive activity with enzymolysis product to the inhibiting rate of ACE.The measure of enzymolysis product ACE inhibitory activity is according to document
" such as Liu Zhiguo, Wu Qiong, Lv Lingxiao digests rice bran protein separation and Extraction ace inhibitory peptide and its structural research [J] food section
Learn, 2007,28 (3)：223~227. " method is carried out, with N- [3- (2- furyls) acryloyl] the sweet ammonia of-L- phenylalanyls
Acyl glycine（FAPGG）As the substrate that ACE is catalyzed, the change of its absorbance is studied, determined using enzyme scalar quantity instrument.
The preparation of embodiment 1, maize germ complex enzyme hydrolysis thing
Raw material maize germ：200g；
Wheat embryo：100g；
Rice germ：50g.
（1）The preparation of compound germ flour
1）Infrared enzyme deactivating is dried
The moisture that fresh three kinds of grain germs adjust grain germ using catalysis type infrared drier is taken, sample is placed on iron
The sample disc that silk screen is made（The cm of diameter 20）In, it is placed in immediately below infrared emittance；Material thickness 3mm, the cm of radiation length 30；
In drying process, example weight passes through the online real time record of balance, according to grain germ initial weight and original water content meter
Calculation draws grain germ final moisture content, and the water content of grain germ is 3% after drying；
2）Color sorting
Dried grain germ is put into color selector, the deleterious particle such as stain particle or other impurities in raw material is by color sorting
Machine is removed substantially, and clearance rate is 95.0%；
3）Ultramicro grinding
Grain germ is carried out into ultramicro grinding, using the broken method of air blast ultramicro powder, is crushed 10 minutes, obtain particle diameter for 25 microns
Uniform and smooth powder, as grain germ powder.
4）Ultrasonic wave added cellulase degradation：In mass ratio it is 1:The grain germ that 5 ratio will be crushed（1000g）With water
（5000g）Suspension is mixed into, 60 DEG C are heated to, pH to 5.5 is adjusted, is digested under the conditions of 60 DEG C using cellulase
200min, enzyme concentration is 400u/g with the mass ratio of substrate according to the enzyme activity of enzyme；Carried out while enzymolysis at double-frequency synchronous ultrasound
Manage, supersonic frequency is：35/28 kHz；The W/L of ultrasonic power density 80；The s of ultrasonic pulse working time 10；Interpulse period 3
s.Ultrasonic time is identical with enzymolysis time；
5）Frequency sweep ultrasonic auxilin enzyme is digested：PH is to 8.5 for regulation, is cooled to 50 DEG C, and Alcalase is added according still further to 800 u/g
Alkali protease, it is ultrasonically treated to carry out double frequency frequency sweep mode rapidly, enzymolysis time 80min.Double frequency frequency sweep ultrasonic is fixed when working
Upper and lower plates spacing is 8cm, frequency sweep cycle 400s, intermittently than 1:1（Ultrasonic 10s, interval 10s）, up and down every piece of vibration plate power be
600W.Double frequency combination of frequency is：33kHz/68kHz.
6）Ultrasonic wave added flavor protease is digested：PH is to 5.5 for regulation, is cooled to 40 DEG C, and local flavor is added according still further to 600u/g
Protease, carries out rapidly adverse current impulse ultrasound assistance enzymolysis, enzymolysis time 120min.Adverse current impulse ultrasound assistance enzymolysis it is specific
Parameter is pulse width 1s, pulse spacing 1s, ultrasonic power density 50W/L；The kHz of supersonic frequency 28, feed liquid is with circulated in countercurrent
Mode passes through ultrasonic probe；
7）After enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 80 DEG C and is kept for 20 minutes, be then cooled to 50 DEG C, and add
It is 1 according to grain germ and maltodextrin:1 mass ratio adds maltodextrin to stir, and is carried out by high pressure homogenizer
Matter three times, each pressure is respectively 40 MPas, and 50 MPas, 55 MPas, homogenizing time is respectively 10 min, 15min, 10
min.Collect enzymolysis liquid several times；Finally it is spray-dried, the process conditions of spray drying are：160 ~ 200 DEG C of intake air temperature,
Air outlet temperature is 70 ~ 100 DEG C, obtains maize germ complex enzyme hydrolysis produce product.Determine the ACE inhibitory activity 71.5% of zymolyte.
The preparation of embodiment 2, maize germ complex enzyme hydrolysis thing
Raw material maize germ： 400g；
Wheat embryo： 150g；
Rice germ： 100g.
（1）The preparation of germ flour
1）Infrared enzyme deactivating is dried
The moisture that fresh plumule adjusts grain germ using catalysis type infrared drier is taken, sample is placed on wire netting and is made
Sample disc（The cm of diameter 20）In, it is placed in immediately below infrared emittance；Material thickness 3mm, the cm of radiation length 30；Drying process
In, example weight calculates plumule most by the online real time record of balance according to plumule initial weight and original water content
Whole moisture, the water content of plumule is 3% after drying；
2）Color sorting
Dried plumule is put into color selector, the deleterious particle such as stain particle or other impurities in raw material is by color selector base
This removing, clearance rate is 95.0%；
3）Ultramicro grinding
Plumule is carried out into ultramicro grinding, using the broken method of air blast ultramicro powder, is crushed 30 minutes, it is 10 microns equal to obtain particle diameter
Even and fine greasy powder, as germ flour.
4）Ultrasonic wave added cellulase degradation：In mass ratio it is 1:The grain germ that 10 ratio will be crushed（1000g）With
Water（10kg）Suspension is mixed into, 60 DEG C are heated to, pH to 5.5 is adjusted, is digested under the conditions of 60 DEG C using cellulase
240min, enzyme concentration is 600u/g with the mass ratio of substrate according to the enzyme activity of enzyme；Carried out while enzymolysis at three frequency synchronizing ultrasounds
Manage, supersonic frequency is：35/28/40 kHz；The W/L of ultrasonic power density 120；The s of ultrasonic pulse working time 10；Pulse interval
The s of time 3.Ultrasonic time is identical with enzymolysis time；
5）Frequency sweep ultrasonic auxilin enzyme is digested：PH is to 8.5 for regulation, is cooled to 60 DEG C, is added according still further to 2000 u/g
Alcalase alkali proteases, it is ultrasonically treated to carry out double frequency frequency sweep mode rapidly, enzymolysis time 200min.Double frequency frequency sweep ultrasonic work
It is 8cm, frequency sweep cycle 400s, intermittently than 1 that upper and lower plates spacing is fixed when making:1（Ultrasonic 10s, interval 10s）, every piece of vibration plate up and down
Power is 600W.Double frequency combination of frequency is：33kHz/68kHz.
6）Ultrasonic wave added flavor protease is digested：PH is to 5.5 for regulation, is cooled to 40 DEG C, and local flavor is added according still further to 5000u/g
Protease, carries out rapidly adverse current impulse ultrasound assistance enzymolysis, the min of enzymolysis time 300.The tool of adverse current impulse ultrasound assistance enzymolysis
Body parameter is pulse width 5s, pulse spacing 5s, ultrasonic power density 100W/L；The kHz of supersonic frequency 28, feed liquid is followed with adverse current
The mode of ring passes through ultrasonic probe；
7）After enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 80 DEG C and is kept for 20 minutes, be then cooled to 50 DEG C, and add
It is 1 according to plumule and maltodextrin:1 mass ratio adds maltodextrin to stir, and homogeneous three is carried out by high pressure homogenizer
Secondary, each pressure is respectively 40 MPas, and 50 MPas, 55 MPas, homogenizing time is respectively 10 min, 15min, 10 min.Receive
Collect enzymolysis liquid several times；Finally it is spray-dried, the process conditions of spray drying are：160 ~ 200 DEG C of intake air temperature, air outlet
Temperature is 70 ~ 100 DEG C, obtains maize germ complex enzyme hydrolysis produce product.Determine the ACE inhibitory activity 69.5% of zymolyte.
Claims (8)
1. maize germ complex enzyme hydrolysis thing, it is characterised in that be prepared from by the raw material of following weight portion：
Maize germ：20~40 parts；
Wheat embryo：10~15 parts；
Rice germ：5~10 parts.
2. the maize germ complex enzyme hydrolysis thing described in claim 1, it is characterised in that be prepared from by the raw material of following weight portion：
Maize germ：40 parts；
Maize germ：15 parts；
Rice germ：10 parts.
3. the preparation method of the maize germ complex enzyme hydrolysis thing described in claim 1-2, it is characterised in that enter as steps described below
Row is prepared：
（1）Plumule stabilization processes；Maize germ, wheat embryo and rice germ are mixed according to aforementioned proportion, takes infrared
Dry, the water content of plumule is less than 3% after treatment；
（2）Color selector is screened；Dried plumule is put into color selector, clearance rate is more than 99%；
（3）Ultramicro grinding：Dried plumule is allowed to broken using air blast ultramicro powder is broken, so as to germ particles be crushed to
10~25 microns of powder；Grinding time is 10~30 minutes；
（4）Ultrasonic wave added cellulase degradation：In mass ratio it is 1:The plumule of crushing and water are mixed into suspension by the ratio of 5-10
Liquid, is heated to 60 DEG C, adjusts pH to 5.5, and 200-240min is digested under the conditions of 60 DEG C using cellulase, and enzyme concentration is according to enzyme
The mass ratio of enzyme activity and substrate be 400-600u/g；Multifrequency mode ultrasound treatment, ultrasonic time and enzyme are carried out while enzymolysis
The solution time is identical；
（5）Frequency sweep ultrasonic auxilin enzyme is digested：PH is to 8.5 for regulation, is cooled to 50 ~ 60 DEG C, adds according still further to 800-2000 u/g
Enter Alcalase alkali proteases, it is ultrasonically treated to carry out double frequency frequency sweep mode rapidly, the min of enzymolysis time 80 ~ 200；
（6）Ultrasonic wave added flavor protease is digested：PH is to 5.5 for regulation, is cooled to 40 DEG C, and wind is added according still further to 600-5000 u/g
Taste protease, carries out rapidly adverse current impulse ultrasound assistance enzymolysis, the min of enzymolysis time 120 ~ 300；
（7）After enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 80 DEG C and is kept for 20 minutes, be then cooled to 50 DEG C, and add
It is 1 to enter according to plumule and maltodextrin:1 mass ratio adds maltodextrin to stir, and homogeneous is carried out by high pressure homogenizer
Three times, each pressure is respectively 40 MPas, and 50 MPas, 55 MPas, homogenizing time is respectively 10 min, 15min, 10 min most
After be spray-dried, the process conditions of spray drying are：160 ~ 200 DEG C of intake air temperature, air outlet temperature is 70 ~ 100 DEG C,
Obtain maize germ complex enzyme hydrolysis produce product.
4. the preparation method of maize germ complex enzyme hydrolysis thing according to claim 3, it is characterised in that step（4）Described
Multiple frequency ultrasonic tupe is：Three frequencies or double-frequency synchronous are ultrasonically treated, the W/L of ultrasonic power density 80 ~ 120；Ultrasonic pulse work
Make the s of time 10；The s of interpulse period 3.
5. the preparation method of maize germ complex enzyme hydrolysis thing according to claim 3, it is characterised in that step（4）Described three
Frequency or the ultrasonically treated ultrasonic frequency of double-frequency synchronous are combined as：35/28 kHz, 35/28/40 kHz or 35/40 kHz.
6. the preparation method of maize germ complex enzyme hydrolysis thing according to claim 3, it is characterised in that wherein step（5）In
It is 8cm, frequency sweep cycle 400s, intermittently than 1 that upper and lower plates spacing is fixed when described double frequency frequency sweep ultrasonic works:1（Ultrasonic 10s,
Have a rest 10s）, every piece of vibration plate power is 600W up and down.
7. the preparation method of maize germ complex enzyme hydrolysis thing according to claim 3, it is characterised in that wherein step（5）In
Described double frequency combination of frequency is： 33 kHz/ 68kHz、33kHz/40kHz、68 kHz/28 kHz；Most preferably good group of frequencies
It is combined into 33 kHz/68 kHz.
8. the preparation method of maize germ complex enzyme hydrolysis thing according to claim 3, it is characterised in that adverse current impulse ultrasound
The design parameter of assistance enzymolysis is 1 ~ 5s of pulse width, 1 ~ 5s of pulse spacing, the W/L of ultrasonic power density 80 ~ 100；Supersonic frequency
28kHz, feed liquid passes through ultrasonic probe in the way of circulated in countercurrent.
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| CN108634313A (en) * | 2018-05-11 | 2018-10-12 | 江苏大学 | A kind of sweet potato stem leaf high nutrition activity extract and preparation method thereof |
| CN113881561A (en) * | 2021-05-07 | 2022-01-04 | 东北农业大学 | Method for producing rice bran protein polypeptide by using adjustable enzyme membrane reactor |
| CN116046516A (en) * | 2023-04-03 | 2023-05-02 | 四川中科高能科技发展有限责任公司 | Food quality improving method based on irradiation parameter automatic screening |
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| CN104774893A (en) * | 2015-03-27 | 2015-07-15 | 江苏大学 | Method of gluten enzymolysis through countercurrent ultrasound treatment |
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| CN108634313A (en) * | 2018-05-11 | 2018-10-12 | 江苏大学 | A kind of sweet potato stem leaf high nutrition activity extract and preparation method thereof |
| CN113881561A (en) * | 2021-05-07 | 2022-01-04 | 东北农业大学 | Method for producing rice bran protein polypeptide by using adjustable enzyme membrane reactor |
| CN116046516A (en) * | 2023-04-03 | 2023-05-02 | 四川中科高能科技发展有限责任公司 | Food quality improving method based on irradiation parameter automatic screening |
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