CN106858342A - Wheat embryo zymolyte compound, preparation method and the purposes as health food - Google Patents
Wheat embryo zymolyte compound, preparation method and the purposes as health food Download PDFInfo
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Abstract
The invention discloses wheat embryo zymolyte compound, preparation method and the purposes as health food, belong to health food development applied technical field.Wheat embryo, maize germ and the rice germ powder of uniform and smooth are prepared by the treatment of infrared and microwave, color selector screening, ultramicro grinding, the complete functional component remained in raw material, meets modern food and pursues natural, nutrition, the overall development trend of health；Crude fibre in wheat embryo, maize germ and rice germ is converted to by dietary fiber, polysaccharide etc. by multi-step biological enzyme resolving tech, corn protein is changed into by activated protein, functional polypeptide, oligopeptides etc., the complete nutritional ingredient remained in grain germ by alkali protease, neutral proteinase and flavor protease etc..During biology enzyme enzymolysis in ultrasonic wave added enzymolysis process, enzymolysis time is shortened, improve hydrolysis result, reduce the usage amount of enzyme, improve the activity of enzymolysis product.
Description
Technical field
The present invention relates to refer in particular to one kind with wheat embryo as primary raw material, using stepwise discretization, ultrasonic wave added enzymolysis, height
Pressure homogenization prepares the method and its purposes as health food of functional polypeptide, belongs to health food development application technology neck
Domain.
Background technology
Cereal (wheat, corn, rice) plumule is the basis of corn growing development, there is high nutritive value.With small
As a example by wheat germ, it accounts for the 2.5% of wheat seed.Containing extremely enrich and the protein of high-quality, fat, multivitamin,
Mineral matter and some not yet clearly micro physiologically active components, be described as " the natural treasure-house of the mankind ", " life of the mankind it
Source ".Protein content is up to more than 30% in wheat germ, and containing 8 kinds of amino acid needed by human, the content of particularly lysine is accounted for
18.5%, amino acid needed for baby's child's growth and development can be supplemented.Wheat germ contains abundant multivitamin, vitamin B1Content
It is 57 times of egg, vitamin B2Content be 2 times of egg, the content of vitamin E is more up to 34.9mg/100g, nutrition
Scholar claims it to be the treasure-house of natural VE, the effect with enhancing brain cell activity, promotes baby's child's brain development.Wheat germ is also
Containing the several mineral materials such as calcium, potassium, magnesium, iron, zinc, chromium, selenium, phosphorus, manganese, copper and trace element, to promoting children to grow
Play an important roll, be a kind of splendid natural minerals element source of supply.Glutathione in wheat germ protein is in human body cell
Played an important role in redox, the formation of internal peroxide can be avoided, with protection brain, promote infant growth
Etc. function.Containing abundant phosphatide, choline in wheat germ, with promotion brain development, deepen memory and raising intelligence etc.
Function.
The starting point that maize germ grows as corn kernel, is lifespring.Though maize germ only accounts for Corn Seeds
The 10%~15% of grain weight, but nutritive value extremely enriches, concentrated in corn kernel more than 80% it is fatty and inorganic
Salt, 60% carbohydrate and 20% high-quality protein, and contain other nutritional ingredients such as phosphatide, sitosterol.Maize germ is still
A kind of protein resource of high-quality, its protein content is average more than 24.3%, is only second to the high-protein plant kind such as soya bean and green soya bean
Son, its composition respectively accounts for 30% or so, glutelin and 5% alcohol in addition with 25% or so based on albumin and globulin
Molten albumen.Corn germ protein is roughly the same with the amino acid composition in whole-egg protein, containing abundant leucine, lysine and
The essential amino acids such as valine, AAS (AAS) is higher than oatmeal and polished rice.It is beautiful compared to other plant albumen
The amino acid of rice germ protein constitutes complete and equilibrium, can meet demand of the human body to various essential amino acids.Maize germ
Albumen is only secondary also with biological value (Biological Value, BV) higher in addition to being constituted with amino acid in a balanced way
In egg (94%) and milk (85%).Defatted corn germ albumen efficiency ratio (Protein Efficiency Ratio,
PER) it is about 2.3, it is close with casein, soybean protein, WHO/FAO recommendations are substantially conformed to, BV values are 70.8%, its Digestive
Number is 80.
On its plumule, plumule contains rich in protein and other nutritional ingredients to the Major Nutrient of rice.Plumule is one
3% is only accounted for by weight in grain rice, but its nutrition accounts for one the 50% of rice, is described as " nutrient source of being bestowed by heaven ".Plumule contains rich
Rich protein and starch, dietary fiber, multivitamin and bioactivator, containing microelements of calcium, iron, zinc, selenium etc..Greatly
More than 20%, natural VE is 200mg/100g-300mg/ in lipid for protein and lipid content in rice plumule
100g, aliphatic acid 70% in lipid contain abundant vitamin and mineral matter above is unrighted acid.Rice germ because
It is containing compared with fattiness, therefore to be usually used to produce rice germ oil.Rice germ oil is natural tocopherol level highest oil product,
Due to being coexisted with a small amount of phytosterol, the action effect of its combined state becomes apparent natural VE.Rice germ protein
Middle amino acid composition is more balanced, and wherein essential amino acid ratio meets the nutrition Reasonable Mode of FAO/WHO suggestions.Cause
This, rice germ protein matter is a kind of good protein for belonging to full price, and its quality can compare favourably with egg.Rice germ it is main by
Albumin, globulin, alcohol soluble protein and glutelin composition, various separation albumen have different processing and functional characters.
The patent of the existing processing technology about above-mentioned several grain germs in the country or research, such as a kind of wheat embryo of patent
The combined preparation process (application number 201110327926) of oil and wheat plantule protein, discloses a kind of wheat embryo in the patent
The combined preparation process of oil and wheat plantule protein, with wheat germ powder as raw material, using supercritical CO2Fluids extraction is extracted
Wheat-germ oil, and with fluid rectification method purify improve wheat embryo grease quality；Low temperature water extraction, ultra-filtration and separation work are used again
Skill extracts the wheat germ protein in the overcritical residue of wheat germ powder, and the wheat-germ oil and wheat germ protein can be widely applied to medicine
Product, health food.Patent " combined preparation process (application number 200710078256) of wheat plantule protein and its derived product ",
With drying wheat plumule as raw material, by techniques such as degreasing, solvent extraction, separation, enzyme hydrolysis, the molten egg of wheat embryo alcohol is prepared
In vain, wheat embryo is anti-oxidant, anti-senility liquid, hydrolyzed wheat plumule peptide concentrate and powder, wheat plantule protein feed addictive etc.
Multiple product, moreover it is possible to the equal reproducible utilization of decolorising agent, ethanol or methanol solvate used during production.
For the research of maize germ, such as a kind of oil-containing corn embryo albumen powder of patent and preparation method thereof (application number
200810006483) fresh maize germ, is produced into low change by low temperature drying, low temperature parch, cold pressing in the patent
The property oil-containing corn embryo dregs of rice；It is raw material with the oil-containing corn embryo dregs of rice for obtaining, oil-containing corn embryo is obtained with the heavy technique of alkali carries acid
Albumen powder.As a kind of suckable maize embryo antioxidative peptide Guiling Jelly of patent processing technology (application number 201310217224),
The preparation method of suckable maize embryo antioxidative peptide Guiling Jelly, including maize germ antioxidant peptide powder 6 are disclosed in the patent
~10 parts, 70~85 parts of mixing of Guiling Jelly pulvis add 100~120 portions of frozen water to mix, stirring to pasty state, and addition 1000~
1200 parts of 40 DEG C of warm water, are heated to 90 DEG C, endure to lotion, sterilized filling, and maize germ anti-oxidation peptide tortoise is after cooling encapsulation
Siberian cocklebur cream finished product, wherein maize germ antioxidant peptide powder are using 2709 alkali proteases, in pH9~11, enzyme-to-substrate concentration ratio
8%~12%, corn germ protein is digested under conditions of concentration of substrate 2%~4%, 50 DEG C~60 DEG C of hydrolysis temperature,
The maize germ antioxidant peptide powder for being obtained through ultrafiltration, freeze-drying again.
For the research of rice germ, a kind of extracting method (application number 201510104665) of rice germ albumen is public
A kind of extracting method of rice germ albumen is opened, has been comprised the following steps：(1) degreasing sample is weighed, after being dissolved in water, in water-bath
Preheated in pot, be put into concussion in constant temperature blender with magnetic force, protein is dissolved in sample liquid, 4000r/min is centrifuged 20 minutes, collects
Supernatant, as albumin；(2) the above-mentioned residue extracted through water is taken, 6% sodium chloride solution is added, fully after dissolving, is pressed
Same method collects supernatant, as globulin；(3) again by the ethanol solution of residue addition 70%, supernatant is collected in dissolving
Liquid, as protein,alcohol-soluble；(4) residue precipitation is added 0.4% NaOH, fully dissolving is in kind obtained paddy
Albumen.
It is above-mentioned to have the following disadvantages in the prior art：
First, processing step is cumbersome, either studies more wheat germ, or studies less rice germ, all uses
Complicated technique is processed, and is substantially and first prepare grain germ oil, the grouts being then left with, step by step arithmetic albumen,
Or extract and digest prepare functional polypeptide again after albumen, processing step is complicated, is unsuitable for technology production, and due to substep
Treatment, product yield is low therefore extremely low for the utilization rate of plumule！
Second, fresh grain germ particle reaches the purpose of enzyme dehumidifying of going out by certain treatment, can just be easy to storage.
But giving birth to China's processing method due to plumule uses the open firing, naked light can well to keep the peculiar taste material of food, but
Environmental pollution can be caused, and efficiency of utilization is low；Meanwhile, special messenger being needed using open firing and is managed, low production efficiency is uncomfortable
For mass producing.
3rd, during existing enzymatic isolation method prepares albumen or polypeptide, because the grain germs such as wheat embryo contain oil
The Multiple components such as fat, starch, cellulose so that its albumen is difficult by protease hydrolyzed, and then cause the degree of hydrolysis of enzymolysis protein
Relatively low, efficiency of pcr product is very low, it is difficult to be processed further utilizing.
The content of the invention
To solve the above problems, the invention is mainly by physical stabilization treatment, color selector screening, ultramicro grinding, many
The technological means such as step enzymolysis, ultrasonic wave added enzymolysis prepare the complex enzyme hydrolysis thing based on wheat embryo, and study its effect and conduct
The possibility of functional food.
Wheat embryo complex enzyme hydrolysis thing of the present invention, is made up of the raw material of following weight portion：
Wheat embryo zymolyte：30~60 parts；
Maize germ zymolyte：20~30 parts；
Rice germ zymolyte：5~15 parts.
It is preferred that being made up of the raw material of following weight portion：
Wheat embryo zymolyte：30 parts；
Maize germ zymolyte：20 parts；
Rice germ zymolyte：5 parts.
Wherein described wheat embryo zymolyte, is prepared obtains as steps described below：
(1) wheat embryo stabilization processes；Infra-red drying is taken, the water content of wheat germ is less than 3% after treatment；
(2) color selector screening；Dried wheat germ is put into color selector, clearance rate is more than 99%；
(3) ultramicro grinding：Dried wheat germ is allowed to broken using air blast ultramicro powder is broken, so as to by wheat germ granular powder
It is broken to 10~25 microns of powder；Grinding time is 5~30 minutes；
(4) ultrasonic wave added cellulase degradation：In mass ratio it is 1:The ratio of 1-10 mixes the wheat embryo of crushing and water
Synthesis suspension, is heated to 60 DEG C, adjusts pH to 5.5, and 120-240min is digested under the conditions of 60 DEG C using cellulase, enzyme-added
It is 100-200u/g to measure according to the enzyme activity of enzyme and the mass ratio of substrate；Multifrequency mode ultrasound treatment is carried out while enzymolysis, ultrasound
Time is identical with enzymolysis time；
Wherein described multiple frequency ultrasonic tupe is：Three frequencies or double-frequency synchronous are ultrasonically treated, ultrasonic power density 40
~100W/L；Ultrasonic pulse working time 10s；Interpulse period 3s.
Three frequencies or the ultrasonically treated ultrasonic frequency of double-frequency synchronous are combined as：20/28kHz, 20/28/40kHz or
28/40kHz。
(5) frequency sweep ultrasonic auxilin enzyme enzymolysis：PH is to 8.5 for regulation, 50~60 DEG C is cooled to, according still further to 500-1800u/
G adds Alcalase alkali proteases, and it is ultrasonically treated to carry out double frequency frequency sweep mode rapidly, 40~120min of enzymolysis time.
Double frequency frequency sweep ultrasonic wherein described in step (5) fixes upper and lower plates spacing when working be 8cm, frequency sweep cycle
400s, intermittently than 1:1 (ultrasonic 10s, interval 10s), every piece of vibration plate power is 600W up and down.
Double frequency combination of frequency wherein described in step (5) is：40kHz/68kHz、33kHz/22kHz、33kHz/
28kHz；Most preferably good combination of frequency is 40kHz/68kHz.
(6) ultrasonic wave added flavor protease enzymolysis：PH is to 5.5 for regulation, is cooled to 40 DEG C, according still further to 400-4000u/g plus
Enter flavor protease, adverse current impulse ultrasound assistance enzymolysis, 60~240min of enzymolysis time are carried out rapidly.
The design parameter of adverse current impulse ultrasound assistance enzymolysis is 1~5s of pulse width, 1~5s of pulse spacing, ultrasonic power
50~80W/L of density；Supersonic frequency 20kHz, feed liquid passes through ultrasonic probe in the way of circulated in countercurrent；
(7) after enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 80 DEG C and is kept for 20 minutes, be then cooled to 50 DEG C,
And it is 1 to add according to wheat embryo and maltodextrin:1 mass ratio adds maltodextrin to stir, by high pressure homogenizer
Carry out homogeneous three times, each pressure is respectively 40 MPas, and 50 MPas, 55 MPas, homogenizing time is respectively 10min, 15min,
10min is finally spray-dried, and the process conditions of spray drying are：160~200 DEG C of intake air temperature, air outlet temperature is
70~100 DEG C, obtain wheat embryo zymolyte product.
Wherein described maize germ zymolyte, is prepared obtains as steps described below：
(1) maize germ stabilization processes；Microwave drying is taken, the water content of maize germ is less than 5% after treatment；
(2) color selector screening；Dried maize germ is put into color selector, clearance rate is more than 99%；
(3) ultramicro grinding：Dried maize germ is allowed to broken using mechanical shearing type ultramicro grinding, so as to by jade
Rice germ particles are crushed to the powder less than 25 microns；Grinding time is 10~30 minutes；
(4) ultrasonic wave added cellulase degradation：In mass ratio it is 1:The ratio of 1-5 mixes the maize germ crushed with water
Into suspension, 55 DEG C are heated to, adjust pH to 6.0,100-200min, enzyme concentration are digested under the conditions of 55 DEG C using cellulase
It is 600-1000u/g according to the enzyme activity of enzyme and the mass ratio of substrate；；Frequency sweep mode is carried out while enzymolysis ultrasonically treated, ultrasound
Time is identical with enzymolysis time；
Double frequency frequency sweep ultrasonic wherein described in step (4) fixes upper and lower plates spacing when working be 10cm, frequency sweep cycle
300s, intermittently than 2:1 (ultrasonic 10s, interval 5s), every piece of vibration plate power is 600W up and down.
Double frequency combination of frequency wherein described in step (4) is：40kHz/22kHz、40kHz/28kHz、40kHz/
33kHz、33kHz/22kHz；Most preferably good combination of frequency is 40kHz/22kHz.
(5) multi-mode ultrasonic wave added protease hydrolyzed：PH is to 7.0 for regulation, 40~50 DEG C is cooled to, according still further to 1800u/g
The neutral proteinase of step (1) is added, multifrequency mode ultrasound treatment, 60~480min of enzymolysis time are carried out rapidly.
Wherein described multiple frequency ultrasonic pattern is：Three frequencies or double-frequency sequence are ultrasonically treated, and ultrasonic power density 30~
150W/L；Ultrasonic pulse working time 10s；Interpulse period 3s.
Three frequencies or the ultrasonically treated ultrasonic frequency of double-frequency sequence are combined as：28/40kHz, 28/35/40kHz or
35/40kHz。
(6) flavor protease enzymolysis：PH is to 6.0 for regulation, is cooled to 45 DEG C, and local flavor egg is added according still further to 1000-1800u/g
White enzyme, is digested, 60~90min of enzymolysis time.
(7) after enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 75 DEG C and is kept for 30 minutes, be then cooled to 55 DEG C,
And it is 1 to add according to maize germ and maltodextrin:1 mass ratio adds maltodextrin to stir, by high pressure homogenizer
Carry out homogeneous three times, each pressure is respectively 50 MPas, and 40 MPas, 50 MPas, homogenizing time is respectively 15min, 20min,
10min is finally spray-dried, and the process conditions of spray drying are：160~200 DEG C of intake air temperature, air outlet temperature is
70~100 DEG C, obtain maize germ zymolyte product.
Wherein described rice germ zymolyte, is prepared obtains as steps described below：
(1) rice germ stabilization processes；Heated-air drying is taken, the water content of rice germ is less than 5% after treatment；
(2) color selector screening；Dried rice germ is put into color selector, clearance rate is more than 99%；
(3) ultramicro grinding：Dried rice germ is allowed to broken using grinding Jie's formula and crush, so as to by rice germ
Grain is crushed to the powder less than 20 microns；Grinding time is 15~20 minutes；
(4) ultrasonic wave added cellulase degradation：In mass ratio it is 1:The ratio of 8-15 mixes the rice germ of crushing and water
Synthesis suspension, is heated to 50 DEG C, adjusts pH to 6.0, and 60-120min is digested under the conditions of 50 DEG C using cellulase, enzyme-added
It is 200-400u/g to measure according to the enzyme activity of enzyme and the mass ratio of substrate；Multifrequency mode ultrasound treatment is carried out while enzymolysis, ultrasound
Time is identical with enzymolysis time；
Wherein described multiple frequency ultrasonic pattern is：Three frequencies or double-frequency synchronous are ultrasonically treated, and ultrasonic power density 30~
150W/L；Ultrasonic pulse working time 10s；Interpulse period 3s.
Three frequencies or the ultrasonically treated ultrasonic frequency of double-frequency synchronous are combined as：35/40kHz, 35/28/40kHz or
28/40kHz。
(5) adverse current impulse ultrasound auxilin enzyme enzymolysis：Regulation pH to 8.0 is cooled to 40~50 DEG C, according still further to 1200u/g
Add neutral proteinase, using adverse current impulse ultrasound assistance enzymolysis, design parameter be 2~10s of pulse width, the pulse spacing 1~
5s, ultrasonic power 80W/L, supersonic frequency 28kHz, feed liquid in the way of circulated in countercurrent by ultrasonic probe, ultrasonic time 45~
90min；
(6) flavor protease enzymolysis：PH is to 6.0 for regulation, is cooled to 45 DEG C, and local flavor egg is added according still further to 900-1200u/g
White enzyme, is digested, 45~60min of enzymolysis time.
(7) after enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 70 DEG C and is kept for 20 minutes, be then cooled to 50 DEG C,
And it is 1 to add according to rice germ and maltodextrin:1 mass ratio adds maltodextrin to stir, by high pressure homogenizer
Carry out homogeneous three times, each pressure is respectively 40 MPas, and 45 MPas, 50 MPas, homogenizing time is respectively 10min, 20min,
30min is finally spray-dried, and the process conditions of spray drying are：160~200 DEG C of intake air temperature, air outlet temperature is
70~100 DEG C, obtain rice germ zymolyte product.
There is the application of above-mentioned wheat embryo complex enzyme hydrolysis thing, wheat embryo complex enzyme hydrolysis thing stronger antioxidation in vitro to live
Property, the food with antioxidation activity, food ingredient or health food can be prepared.
The beneficial effects of the invention are as follows：
First, the present invention is processed by infrared and microwave, color selector is screened, ultramicro grinding prepares uniform and smooth
Wheat embryo, maize germ and rice germ powder, completely remain the functional component in raw material, meet modern food and pursue day
So, nutrition, the overall development trend of health；
Second, the crude fibre in wheat embryo, maize germ and rice germ is turned by multi-step biological enzyme resolving tech
Dietary fiber, polysaccharide etc. are changed to, corn protein is converted by alkali protease, neutral proteinase and flavor protease etc.
Viability albumen, functional polypeptide, oligopeptides etc., the complete nutritional ingredient remained in grain germ.
3rd, biology enzyme is applied with multi-mode ultrasonic wave, frequency sweep ultrasonic ripple and counter current ultrasonic wave during digesting, ultrasound
During assistance enzymolysis, enzymolysis time is shortened, improve hydrolysis result, reduce the usage amount of enzyme, improve enzymolysis product
Activity.
Brief description of the drawings
Fig. 1 is multi-mode ultrasonic wave added enzymolysis equipment structure chart, wherein 1,2,3 is ultrasonic vibrating plate, 4 to contain liquid device, and 5 is water
Bath, 6 is temp probe, and 7 is circulating pump, and 8 is Computerized controller, and 9,10,11 is ultrasonic controller.
Fig. 2 is the equipment drawing of frequency sweep double-frequency ultrasound pretreatment unit, 1- ultrasounds pond, 2- thermometers, 3- thermostatted water bathing pools, 4-
Vibration plate on ultrasonic wave, vibration plate under 5- ultrasonic waves, 6- sample treatments region, 7- computer controllers, 8- supersonic generators.
Fig. 3 is adverse current ultrasound processing equipment structural representation, and 1 pops one's head in for energy-gathered ultrasonic, and 2 is ultrasonic wave controller, 3
It is ultrasonic cup type chamber, 4 is charging aperture, and 5 is circulating pump, and 6 to contain liquid device, and 7 is discharging opening.
Specific embodiment
The term for being used in the present invention, unless otherwise specified, typically has those of ordinary skill in the art usual
The implication of understanding.With reference to specific embodiment, and the present invention is described in further detail with reference to data.It should be understood that these
Embodiment is of the invention solely for the purpose of illustration, rather than limits the scope of the present invention by any way.
The enzyme used in the present invention, including：
Alcalase2.4LFG protease (1.325 × 105U/g, optimum temperature, 50 DEG C；PH, 8.0), the biological skill of Novi's letter
Art Co., Ltd；Cellulase：The 000U/g of enzyme activity 8, neutral proteinase (enzyme activity is 16.0 ten thousand U/g), flavor protease (enzyme
Vigor is 6.9 ten thousand U/g) provided by Wuxi enzyme preparation Co., Ltd of Jie Neng sections.
Fig. 1 is the multi-mode ultrasonic wave added enzymolysis equipment that ultrasonic wave added enzymatic isolation method of the invention is used, and the equipment is furnished with one
Platform Computerized controller 8, can set ultrasound works parameter (ultrasonic power density, frequency, pulse working time, intermittent time
With treatment total time) control three ultrasonic controllers 9,10,11 respectively, connect respectively three ultrasonic vibrating plates of different frequency 1,2,
3, it is capable of achieving the treatment of single-frequency/two frequency/tri- frequency ultrasonic wave；The aaerosol solution input of enzymolysis will be needed to contain liquid device 4
In carry out the experiment of single-frequency/double frequency/multiple frequency ultrasonic assistance enzymolysis, start circulating pump 7 and enzymolysis liquid be circulated.By water-bath 5
Automatically controlling for enzymolysis liquid temperature is realized with temp probe 6.
Fig. 2 is the equipment drawing of the frequency sweep double-frequency ultrasound pretreatment unit that the present invention is used, and is Jiangsu University's independent development.It is super
Acoustic generator 8 can send the ultrasonic wave for determining frequency and frequency sweep both of which, and separate unit supersonic generator power is 600w.In ultrasonic pond
Placement ultrasonic wave vibration plate upper plate 4 symmetrical above and below and ultrasonic wave lower plate 5, are controlled on ultrasonic wave vibration plate by supersonic generator 8 in 1
Plate 4 and ultrasonic wave lower plate 5；After the setting each parameter of ultrasonic wave of computer controller 7, supersonic generator 8 is controlled, send and meet the requirements
Ultrasonic wave；3 is the thermostatted water bathing pool of equipment in the present invention, by the monitor in real time temperature of thermometer 2, and is adjusted according to need of work
Section medium temperature；Need to liquid raw material to be processed be placed in sample sack to be placed in ultrasonication carried out in treatment fluid region 6.
Fig. 3 is adverse current ultrasound processing equipment of the invention, and the equipment is furnished with ultrasonic wave controller 2, controls ultrasound parameter, gathers
The material that energy formula ultrasonic probe 1 enters ultrasonic cup type chamber 3 to charging aperture 4 is processed, and circulating pump 5 pairs is started while ultrasonic
Feed liquid is circulated, and 6 to contain liquid device, and 7 is discharging opening.
The preparation of embodiment 1, wheat embryo complex enzyme hydrolysis thing
(1) preparation of wheat germ powder
1) infrared enzyme deactivating is dried
The moisture that fresh wheat germ adjusts wheat embryo using catalysis type infrared drier is taken, sample is placed on iron
In the sample disc (diameter 20cm) that silk screen is made, it is placed in immediately below infrared emittance；Material thickness 3mm, radiation length 30cm；It is dry
During dry, example weight is calculated by the online real time record of balance according to wheat germ initial weight and original water content
Wheat germ final moisture content, the water content of wheat germ is 3% after drying；
2) color sorting
Dried wheat embryo is put into color selector, the deleterious particle quilt such as stain particle or other impurities in raw material
Color selector is removed substantially, and clearance rate is 95.0%；
3) ultramicro grinding
Wheat embryo is carried out into ultramicro grinding, using the broken method of air blast ultramicro powder, is crushed 5 minutes, it is 25 to obtain particle diameter
The uniform and smooth powder of micron, as wheat germ powder.
4) ultrasonic wave added cellulase degradation：In mass ratio it is 1:1 ratio by crush wheat embryo (1000g) and water
(1000g) is mixed into suspension, is heated to 60 DEG C, adjusts pH to 5.5, is digested under the conditions of 60 DEG C using cellulase
120min, enzyme concentration is 100u/g with the mass ratio of substrate according to the enzyme activity of enzyme；Carried out while enzymolysis at double-frequency synchronous ultrasound
Manage, supersonic frequency is：20/28kHz；Ultrasonic power density 40W/L；Ultrasonic pulse working time 10s；Interpulse period 3s.
Ultrasonic time is identical with enzymolysis time；
5) frequency sweep ultrasonic auxilin enzyme enzymolysis：PH is to 8.5 for regulation, is cooled to 50 DEG C, is added according still further to 500u/g
Alcalase alkali proteases, it is ultrasonically treated to carry out double frequency frequency sweep mode rapidly, enzymolysis time 40min.Double frequency frequency sweep ultrasonic work
It is 8cm, frequency sweep cycle 400s, intermittently than 1 that upper and lower plates spacing is fixed when making:1 (ultrasonic 10s, interval 10s), up and down every piece of vibration plate
Power is 600W.Double frequency combination of frequency is：40kHz/68kHz.
6) ultrasonic wave added flavor protease enzymolysis：PH is to 5.5 for regulation, is cooled to 40 DEG C, and local flavor is added according still further to 400u/g
Protease, carries out rapidly adverse current impulse ultrasound assistance enzymolysis, enzymolysis time 60min.Adverse current impulse ultrasound assistance enzymolysis it is specific
Parameter is pulse width 1s, pulse spacing 1s, ultrasonic power density 50W/L；Supersonic frequency 20kHz, feed liquid is with circulated in countercurrent
Mode passes through ultrasonic probe；
7) after enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 80 DEG C and is kept for 20 minutes, be then cooled to 50 DEG C, and
It is 1 to add according to wheat embryo and maltodextrin:1 mass ratio adds maltodextrin to stir, and is entered by high pressure homogenizer
Row homogeneous three times, each pressure is respectively 40 MPas, and 50 MPas, 55 MPas, homogenizing time is respectively 10min, 15min,
10min.Collect enzymolysis liquid several times；Finally it is spray-dried, the process conditions of spray drying are：Intake air temperature 160~200
DEG C, air outlet temperature is 70~100 DEG C, obtains wheat embryo zymolyte product.
(2) preparation of maize germ zymolyte：
1) maize germ stabilization processes；Microwave drying is taken, maize germ uniformly remains material bed and is uniformly paved into
Thickness 1cm thickness layer, to ensure to be heated evenly, carries out heating using microwave and goes out enzyme while microwave drying, microwave rated power 2kW, place
Reason amount about 30kg maize germs per hour；The water content of maize germ is less than 5% after treatment；
2) color selector screening；Dried maize germ is put into color selector, clearance rate is more than 99%；
3) ultramicro grinding：Dried maize germ is allowed to broken using mechanical shearing type ultramicro grinding, so as to by jade
Rice germ particles are crushed to the powder less than 25 microns；Grinding time is 10 minutes；
4) ultrasonic wave added cellulase degradation：In mass ratio it is 1:Maize germ (1000g) and water that 1 ratio will be crushed
(1000g) is mixed into suspension, is heated to 55 DEG C, adjusts pH to 6.0, is digested under the conditions of 55 DEG C using cellulase
100min, enzyme concentration is 600u/g with the mass ratio of substrate according to the enzyme activity of enzyme；Carried out while enzymolysis at frequency sweep mode ultrasound
Reason, it is 10cm, frequency sweep cycle 300s, intermittently than 2 that upper and lower plates spacing is fixed when double frequency frequency sweep ultrasonic works:1 (ultrasonic 10s, interval
5s), every piece of vibration plate power is 600W up and down.Double frequency combination of frequency is：40kHz/22kHz.Ultrasonic time is identical with enzymolysis time；
5) multi-mode ultrasonic wave added protease hydrolyzed：PH is to 7.0 for regulation, is cooled to 40 DEG C, in being added according still further to 1800u/g
Property protease, carry out multifrequency mode ultrasound treatment rapidly, the ultrasonically treated ultrasonic frequencies of three frequency orders are combined as：28/35/
40kHz.Ultrasonic power density 30W/L；Ultrasonic pulse working time 10s；Interpulse period 3s.Enzymolysis time 60min.
6) flavor protease enzymolysis：PH is to 6.0 for regulation, is cooled to 45 DEG C, and flavor protease is added according still further to 1000u/g,
Digested, enzymolysis time 60min.
7) after enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 75 DEG C and is kept for 30 minutes, be then cooled to 55 DEG C, and
It is 1 to add according to maize germ and maltodextrin:1 mass ratio adds maltodextrin to stir, and is entered by high pressure homogenizer
Row homogeneous three times, each pressure is respectively 50 MPas, and 40 MPas, 50 MPas, homogenizing time is respectively 15min, 20min,
10min；Enzymolysis liquid several times is collected, is finally spray-dried, the process conditions of spray drying are：Intake air temperature 160~
200 DEG C, air outlet temperature is 70~100 DEG C, obtains maize germ zymolyte product.
(3) preparation of rice germ zymolyte：
1) heated-air drying
Take rice germ to be put into hot air drier, using 50 DEG C of heated-air dryings, the rice germ moisture for obtaining is
5%；
2) color sorting
To be put into color selector by the rice germ after heated-air drying, stain particle or other impurities in raw material etc. have
Evil particle is removed substantially by color selector, and clearance rate is 95.0%；
3) ultramicro grinding：Dried rice germ is allowed to broken using grinding Jie's formula and crush, so as to by rice germ
Grain is crushed to the powder less than 20 microns；Grinding time is 15 minutes；
4) ultrasonic wave added cellulase degradation：In mass ratio it is 1:Rice germ (200g) and water that 8 ratio will be crushed
(1600g) is mixed into suspension, is heated to 50 DEG C, adjusts pH to 6.0, and 60min is digested under the conditions of 50 DEG C using cellulase,
Enzyme concentration is 200u/g with the mass ratio of substrate according to the enzyme activity of enzyme；Multifrequency mode ultrasound treatment, three frequencies are carried out while enzymolysis
The ultrasonic frequency of synchronizing ultrasound treatment, three frequencies or synchronizing ultrasound treatment is combined as：35/28/40kHz.Ultrasonic power density
30W/L；Ultrasonic pulse working time 10s；Interpulse period 3s.Ultrasonic time is identical with enzymolysis time；
5) adverse current impulse ultrasound auxilin enzyme enzymolysis：Regulation pH to 8.0 be cooled to 40 DEG C, according still further to 1200u/g plus in
Property protease, using adverse current impulse ultrasound assistance enzymolysis, design parameter is pulse width 2s, pulse spacing 1s, ultrasonic power
80W/L, supersonic frequency 28kHz, feed liquid is in the way of circulated in countercurrent by ultrasonic probe, ultrasonic time 45min；
6) flavor protease enzymolysis：PH is to 6.0 for regulation, is cooled to 45 DEG C, and flavor protease is added according still further to 900u/g, enters
Row enzymolysis, enzymolysis time 45min.
7) after enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 70 DEG C and is kept for 20 minutes, be then cooled to 50 DEG C, and
It is 1 to add according to rice germ and maltodextrin:1 mass ratio adds maltodextrin to stir, and is entered by high pressure homogenizer
Row homogeneous three times, each pressure is respectively 40 MPas, and 45 MPas, 50 MPas, homogenizing time is respectively 10min, 20min,
30min；Zymolyte several times is collected, is finally spray-dried, the process conditions of spray drying are：Intake air temperature 160~200
DEG C, air outlet temperature is 70~100 DEG C, obtains rice germ zymolyte product.
Take above-mentioned wheat embryo zymolyte：300g；Maize germ zymolyte：200g；Rice germ zymolyte：50g.Mixing
Packaging can obtain the grain germ zymolyte functional food based on wheat embryo zymolyte.
The preparation of embodiment 2, wheat embryo complex enzyme hydrolysis thing
(1) preparation of wheat germ powder
1) infrared enzyme deactivating is dried
The moisture that fresh wheat germ adjusts wheat embryo using catalysis type infrared drier is taken, sample is placed on iron
In the sample disc (diameter 20cm) that silk screen is made, it is placed in immediately below infrared emittance；Material thickness 3mm, radiation length 30cm；It is dry
During dry, example weight is calculated by the online real time record of balance according to wheat germ initial weight and original water content
Wheat germ final moisture content, the water content of wheat germ is 3% after drying；
2) color sorting
Dried wheat embryo is put into color selector, the deleterious particle quilt such as stain particle or other impurities in raw material
Color selector is removed substantially, and clearance rate is 95.0%；
3) ultramicro grinding
Wheat embryo is carried out into ultramicro grinding, using the broken method of air blast ultramicro powder, is crushed 30 minutes, it is 10 to obtain particle diameter
The uniform and smooth powder of micron, as wheat germ powder.
4) ultrasonic wave added cellulase degradation：In mass ratio it is 1:10 ratio will crush wheat embryo (1000g) with
Water (10kg) is mixed into suspension, is heated to 60 DEG C, adjusts pH to 5.5, is digested under the conditions of 60 DEG C using cellulase
240min, enzyme concentration is 200u/g with the mass ratio of substrate according to the enzyme activity of enzyme；Carried out while enzymolysis at three frequency synchronizing ultrasounds
Manage, supersonic frequency is：20/28/40kHz；Ultrasonic power density 100W/L；Ultrasonic pulse working time 10s；Interpulse period
3s.Ultrasonic time is identical with enzymolysis time；
5) frequency sweep ultrasonic auxilin enzyme enzymolysis：PH is to 8.5 for regulation, is cooled to 60 DEG C, is added according still further to 1800u/g
Alcalase alkali proteases, it is ultrasonically treated to carry out double frequency frequency sweep mode rapidly, enzymolysis time 120min.Double frequency frequency sweep ultrasonic work
It is 8cm, frequency sweep cycle 400s, intermittently than 1 that upper and lower plates spacing is fixed when making:1 (ultrasonic 10s, interval 10s), up and down every piece of vibration plate
Power is 600W.Double frequency combination of frequency is：33kHz/28kHz.
6) ultrasonic wave added flavor protease enzymolysis：PH is to 5.5 for regulation, is cooled to 40 DEG C, and local flavor is added according still further to 4000u/g
Protease, carries out rapidly adverse current impulse ultrasound assistance enzymolysis, enzymolysis time 240min.Adverse current impulse ultrasound assistance enzymolysis it is specific
Parameter is pulse width 5s, pulse spacing 5s, ultrasonic power density 80W/L；Supersonic frequency 20kHz, feed liquid is with circulated in countercurrent
Mode passes through ultrasonic probe；
7) after enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 80 DEG C and is kept for 20 minutes, be then cooled to 50 DEG C, and
It is 1 to add according to wheat embryo and maltodextrin:1 mass ratio adds maltodextrin to stir, and is entered by high pressure homogenizer
Row homogeneous three times, each pressure is respectively 40 MPas, and 50 MPas, 55 MPas, homogenizing time is respectively 10min, 15min,
10min.Collect enzymolysis liquid several times；Finally it is spray-dried, the process conditions of spray drying are：Intake air temperature 160~200
DEG C, air outlet temperature is 70~100 DEG C, obtains wheat embryo zymolyte product.
(2) preparation of maize germ zymolyte：
1) maize germ stabilization processes；Microwave drying is taken, maize germ uniformly remains material bed and is uniformly paved into
Thickness 1cm thickness layer, to ensure to be heated evenly, carries out heating using microwave and goes out enzyme while microwave drying, microwave rated power 2kW, place
Reason amount about 30kg maize germs per hour；The water content of maize germ is less than 5% after treatment；
2) color selector screening；Dried maize germ is put into color selector, clearance rate is more than 99%；
3) ultramicro grinding：Dried maize germ is allowed to broken using mechanical shearing type ultramicro grinding, so as to by jade
Rice germ particles are crushed to the powder less than 25 microns；Grinding time is 30 minutes；
4) ultrasonic wave added cellulase degradation：In mass ratio it is 1:Maize germ (1000g) and water that 1 ratio will be crushed
(1000g) is mixed into suspension, is heated to 55 DEG C, adjusts pH to 6.0, is digested under the conditions of 55 DEG C using cellulase
100min, enzyme concentration is 600u/g with the mass ratio of substrate according to the enzyme activity of enzyme；Carried out while enzymolysis at frequency sweep mode ultrasound
Reason, it is 10cm, frequency sweep cycle 300s, intermittently than 2 that upper and lower plates spacing is fixed when double frequency frequency sweep ultrasonic works:1 (ultrasonic 10s, interval
5s), every piece of vibration plate power is 600W up and down.Double frequency combination of frequency is：40kHz/22kHz.Ultrasonic time is identical with enzymolysis time；
5) multi-mode ultrasonic wave added protease hydrolyzed：PH is to 7.0 for regulation, is cooled to 40 DEG C, in being added according still further to 1800u/g
Property protease, carry out multifrequency mode ultrasound treatment rapidly, the ultrasonically treated ultrasonic frequencies of three frequency orders are combined as：28/35/
40kHz.Ultrasonic power density 30W/L；Ultrasonic pulse working time 10s；Interpulse period 3s.Enzymolysis time 60min.
6) flavor protease enzymolysis：PH is to 6.0 for regulation, is cooled to 45 DEG C, and flavor protease is added according still further to 1000u/g,
Digested, enzymolysis time 60min.
7) after enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 75 DEG C and is kept for 30 minutes, be then cooled to 55 DEG C, and
It is 1 to add according to maize germ and maltodextrin:1 mass ratio adds maltodextrin to stir, and is entered by high pressure homogenizer
Row homogeneous three times, each pressure is respectively 50 MPas, and 40 MPas, 50 MPas, homogenizing time is respectively 15min, 20min,
10min；Enzymolysis liquid several times is collected, is finally spray-dried, the process conditions of spray drying are：Intake air temperature 160~
200 DEG C, air outlet temperature is 70~100 DEG C, obtains maize germ zymolyte product.
(3) preparation of rice germ zymolyte：
1) heated-air drying
Take rice germ to be put into hot air drier, using 50 DEG C of heated-air dryings, the rice germ moisture for obtaining is
5%；
2) color sorting
To be put into color selector by the rice germ after heated-air drying, stain particle or other impurities in raw material etc. have
Evil particle is removed substantially by color selector, and clearance rate is 95.0%；
3) ultramicro grinding：Dried rice germ is allowed to broken using grinding Jie's formula and crush, so as to by rice germ
Grain is crushed to the powder less than 20 microns；Grinding time is 20 minutes；
4) ultrasonic wave added cellulase degradation：In mass ratio it is 1:Rice germ (200g) and water that 15 ratio will be crushed
(3000g) is mixed into suspension, is heated to 50 DEG C, adjusts pH to 6.0, is digested under the conditions of 50 DEG C using cellulase
120min, enzyme concentration is 400u/g with the mass ratio of substrate according to the enzyme activity of enzyme；Carried out at multifrequency mode ultrasound while enzymolysis
Reason, double-frequency synchronous are ultrasonically treated, and the ultrasonically treated ultrasonic frequency of double-frequency synchronous is combined as：35/40kHz.Ultrasonic power density
30W/L；Ultrasonic pulse working time 10s；Interpulse period 3s.Ultrasonic time is identical with enzymolysis time；
5) adverse current impulse ultrasound auxilin enzyme enzymolysis：Regulation pH to 8.0 be cooled to 50 DEG C, according still further to 1200u/g plus in
Property protease, using adverse current impulse ultrasound assistance enzymolysis, design parameter is pulse width 10s, pulse spacing 5s, ultrasonic power
80W/L, supersonic frequency 28kHz, feed liquid is in the way of circulated in countercurrent by ultrasonic probe, ultrasonic time 90min；
6) flavor protease enzymolysis：PH is to 6.0 for regulation, is cooled to 45 DEG C, and flavor protease is added according still further to 1200u/g,
Digested, enzymolysis time 60min.
7) after enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 70 DEG C and is kept for 20 minutes, be then cooled to 50 DEG C, and
It is 1 to add according to rice germ and maltodextrin:1 mass ratio adds maltodextrin to stir, and is entered by high pressure homogenizer
Row homogeneous three times, each pressure is respectively 40 MPas, and 45 MPas, 50 MPas, homogenizing time is respectively 10min, 20min,
30min；Zymolyte several times is collected, is finally spray-dried, the process conditions of spray drying are：Intake air temperature 160~200
DEG C, air outlet temperature is 70~100 DEG C, obtains rice germ zymolyte product.
Take above-mentioned wheat embryo zymolyte：600g；Maize germ zymolyte：300g；Rice germ zymolyte：150g.It is mixed
Conjunction packaging can obtain the grain germ zymolyte functional food based on wheat embryo zymolyte.
Test wheat embryo zymolyte antioxidation activity experiment：
1st, sample pretreatment step：1g wheat embryo zymolytes are taken respectively, and concussion is uniformly dissolved in 10mL distilled water, 3000
Turn/min under 10min is centrifuged, take supernatant, be diluted to same concentration (1mg/mL), determine its antioxidation activity.
2nd, method：Using ABTS+ free radical scavenging activities, three indexs of DPPH clearance rates and iron ion sequestering power judge
The antioxidation activity of wheat embryo zymolyte.
(1)ABTS+Free radical scavenging activity：
Reagent is configured：7mM ABTS storing solutions and 2.45mM potassium persulfates (ultimate density) are mixed.Under room temperature, lucifuge
12-16h is stood, ABTS is formed+Free radical working solution；Using preceding use 0.2M phosphate buffers (pH 7.4) dilute, make its
Absorbance is 0.70 ± 0.02 at 734nm.
Standard curve：The ABTS of the Trolox (0-3mM) and 1980 μ L of 20 μ L+Concussion is mixed, after reacting 10min at 30 DEG C
Absorbance is surveyed in 734nm.Simultaneously with ultra-pure water as blank, display ABTS is set up+Free radical scavenging activity and Trolox concentration it
Between relation standard curve, formula is as follows.
In formula：Ab- Blank absorbance values；
As- sample absorbance value；
Sample analysis：20 μ L samples (concentration is adjusted according to activity, makes clearance rate in standard curve range) and 1980 μ L
ABTS+Concussion mix, react 10min at 30 DEG C, after 734nm at survey absorbance, with ultra-pure water as blank, calculating ABTS+
Free radical scavenging activity, control mark song obtains representing result with Trolox equivalents (μM).
(2) DPPH clearance rates
Reagent is configured：The solution of 1mmol/L is made into anhydrous alcohol solution DPPH, lucifuge refrigeration is placed.Nothing when using
Water-ethanol dilutes 10 times and uses (0.1mmol/L) afterwards.
Sample determination：1.5mL samples are mixed in 30 DEG C of conditions with the DPPH ethanol solutions concussion of isometric 0.1mmol/L
After lower reaction 30min at 517nm mensuration absorbance value.Meanwhile, DPPH clearance rates, formula are calculated as blank using sample solvent
It is as follows.
In formula：Ab- Blank absorbance values；
As- sample absorbance value；
(3) iron ion sequestering power：
Reagent：2mM FeCl2Solution, 5mM ferrozine solution
Step：Take the 2mM FeCl of 1mL sample solutions and 0.05mL2Solution, 1.85mL ultra-pure waters mixing, reacts 3min
Afterwards；The 5mM ferrozine solution of 0.1mL is added, 10min is reacted under the conditions of 30 DEG C after uniform mixing；Determined under 562nm
The absorbance of reaction solution, and compared with ultra-pure water, formula is as follows.
In formula：Ab- Blank absorbance values；
As- sample absorbance value；
3rd, result of the test
Wheat embryo complex enzyme hydrolysis thing has antioxidation activity, 1mg/mL wheat embryo complex enzyme hydrolysis things pair in the present invention
ABTS+Free radical scavenging activity (%) reaches 46.2%；DPPH free radical scavenging activities reach 71.1%, and iron ion chelation percent is obtained
84.4%；Illustrate that wheat embryo complex enzyme hydrolysis thing has very strong oxidation resistance.Wheat embryo zymolyte can be prepared into is
Main grain germ zymolyte functional food.
Claims (9)
1. wheat embryo complex enzyme hydrolysis thing, it is characterised in that be made up of the raw material of following weight portion：
Wheat embryo zymolyte：30~60 parts；
Maize germ zymolyte：20~30 parts；
Rice germ zymolyte：5~15 parts.
2. wheat embryo complex enzyme hydrolysis thing according to claim 1, it is characterised in that be made up of the raw material of following weight portion：
Wheat embryo zymolyte：30 parts；
Maize germ zymolyte：20 parts；
Rice germ zymolyte：5 parts.
3. wheat embryo complex enzyme hydrolysis thing according to claim 1, it is characterised in that wherein described wheat embryo enzymolysis
Thing, is prepared obtains as steps described below：
（1）Wheat embryo stabilization processes；Infra-red drying is taken, the water content of wheat germ is less than 3% after treatment；
（2）Color selector is screened；Dried wheat germ is put into color selector, clearance rate is more than 99%；
（3）Ultramicro grinding：Dried wheat germ is allowed to broken using air blast ultramicro powder is broken, so as to wheat germ particle be crushed to
10~25 microns of powder；Grinding time is 5~30 minutes；
（4）Ultrasonic wave added cellulase degradation：In mass ratio it is 1:Be mixed into for the wheat embryo of crushing and water by the ratio of 1-10
Suspension, is heated to 60 DEG C, adjusts pH to 5.5, and 120-240min is digested under the conditions of 60 DEG C using cellulase, and enzyme concentration is pressed
It is 100-200u/g according to the enzyme activity of enzyme and the mass ratio of substrate；Multifrequency mode ultrasound treatment, ultrasonic time are carried out while enzymolysis
It is identical with enzymolysis time；
（5）Frequency sweep ultrasonic auxilin enzyme is digested：PH is to 8.5 for regulation, is cooled to 50 ~ 60 DEG C, adds according still further to 500-1800 u/g
Enter Alcalase alkali proteases, it is ultrasonically treated to carry out double frequency frequency sweep mode rapidly, the min of enzymolysis time 40 ~ 120；
（6）Ultrasonic wave added flavor protease is digested：PH is to 5.5 for regulation, is cooled to 40 DEG C, and wind is added according still further to 400-4000 u/g
Taste protease, carries out rapidly adverse current impulse ultrasound assistance enzymolysis, the min of enzymolysis time 60 ~ 240；
（7）After enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 80 DEG C and is kept for 20 minutes, be then cooled to 50 DEG C, and add
It is 1 to enter according to wheat embryo and maltodextrin:1 mass ratio adds maltodextrin to stir, and is carried out by high pressure homogenizer
Homogeneous three times, each pressure is respectively 40 MPas, and 50 MPas, 55 MPas, homogenizing time is respectively 10 min, 15min, 10
Min is finally spray-dried, and the process conditions of spray drying are：160 ~ 200 DEG C of intake air temperature, air outlet temperature be 70 ~
100 DEG C, obtain wheat embryo zymolyte product.
4. wheat embryo complex enzyme hydrolysis thing according to claim 1, it is characterised in that wherein described maize germ enzymolysis
Thing, is prepared obtains as steps described below：
（1）Maize germ stabilization processes；Microwave drying is taken, the water content of maize germ is less than 5% after treatment；
（2）Color selector is screened；Dried maize germ is put into color selector, clearance rate is more than 99%；
（3）Ultramicro grinding：Dried maize germ is allowed to broken using mechanical shearing type ultramicro grinding, so as to by maize
Bud particle is crushed to the powder less than 25 microns；Grinding time is 10~30 minutes；
（4）Ultrasonic wave added cellulase degradation：In mass ratio it is 1:Be mixed into for the maize germ of crushing and water outstanding by the ratio of 1-5
Supernatant liquid, is heated to 55 DEG C, adjusts pH to 6.0, and 100-200min are digested under the conditions of 55 DEG C using cellulase, and enzyme concentration is pressed
It is 600-1000u/g according to the enzyme activity of enzyme and the mass ratio of substrate；；Frequency sweep mode is carried out while enzymolysis ultrasonically treated, when ultrasonic
Between it is identical with enzymolysis time；
（5）Multi-mode ultrasonic wave added protease hydrolyzed：PH is to 7.0 for regulation, is cooled to 40 ~ 50 DEG C, is added according still further to 1800 u/g
Step（1）Neutral proteinase, multifrequency mode ultrasound treatment, the min of enzymolysis time 60 ~ 480 are carried out rapidly；
（6）Flavor protease is digested：PH is to 6.0 for regulation, is cooled to 45 DEG C, and local flavor albumen is added according still further to 1000-1800 u/g
Enzyme, is digested, the min of enzymolysis time 60 ~ 90；
（7）After enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 75 DEG C and is kept for 30 minutes, be then cooled to 55 DEG C, and add
It is 1 to enter according to maize germ and maltodextrin:1 mass ratio adds maltodextrin to stir, and is carried out by high pressure homogenizer
Homogeneous three times, each pressure is respectively 50 MPas, and 40 MPas, 50 MPas, homogenizing time is respectively 15 min, 20min, 10
Min is finally spray-dried, and the process conditions of spray drying are：160 ~ 200 DEG C of intake air temperature, air outlet temperature be 70 ~
100 DEG C, obtain maize germ zymolyte product.
5. wheat embryo complex enzyme hydrolysis thing according to claim 1, it is characterised in that wherein described rice germ enzymolysis
Thing, is prepared obtains as steps described below：
（1）Rice germ stabilization processes；Heated-air drying is taken, the water content of rice germ is less than 5% after treatment；
（2）Color selector is screened；Dried rice germ is put into color selector, clearance rate is more than 99%；
（3）Ultramicro grinding：Dried rice germ is allowed to broken using grinding Jie's formula and crush, so as to by rice germ granular powder
It is broken to the powder less than 20 microns；Grinding time is 15~20 minutes；
（4）Ultrasonic wave added cellulase degradation：In mass ratio it is 1:Be mixed into for the rice germ of crushing and water by the ratio of 8-15
Suspension, is heated to 50 DEG C, adjusts pH to 6.0, and 60-120min is digested under the conditions of 50 DEG C using cellulase, and enzyme concentration is pressed
It is 200-400u/g according to the enzyme activity of enzyme and the mass ratio of substrate；Multifrequency mode ultrasound treatment, ultrasonic time are carried out while enzymolysis
It is identical with enzymolysis time；
（5）Adverse current impulse ultrasound auxilin enzyme is digested：Regulation pH to 8.0 is cooled to 40 ~ 50 DEG C, is added according still further to 1200 u/g
Neutral proteinase, using adverse current impulse ultrasound assistance enzymolysis,
（6）Flavor protease is digested：PH is to 6.0 for regulation, is cooled to 45 DEG C, and local flavor albumen is added according still further to 900-1200 u/g
Enzyme, is digested, the min of enzymolysis time 45 ~ 60；
（7）After enzyme digestion reaction terminates, enzymolysis liquid is brought rapidly up to 70 DEG C and is kept for 20 minutes, be then cooled to 50 DEG C, and add
It is 1 to enter according to rice germ and maltodextrin:1 mass ratio adds maltodextrin to stir, and is carried out by high pressure homogenizer
Homogeneous three times, each pressure is respectively 40 MPas, and 45 MPas, 50 MPas, homogenizing time is respectively 10 min, 20min,
30min is finally spray-dried, and the process conditions of spray drying are：160 ~ 200 DEG C of intake air temperature, air outlet temperature is 70
~ 100 DEG C, obtain rice germ zymolyte product.
6. wheat embryo complex enzyme hydrolysis thing according to claim 3, it is characterised in that wherein described multiple frequency ultrasonic treatment
Pattern is：Three frequencies or double-frequency synchronous are ultrasonically treated, the W/L of ultrasonic power density 40 ~ 100；The s of ultrasonic pulse working time 10；
The s of interpulse period 3；Three frequencies or the ultrasonically treated ultrasonic frequency of double-frequency synchronous are combined as：20/28 kHz、20/28/40
KHz or 28/40 kHz；
Wherein step（5）Described in double frequency frequency sweep ultrasonic upper and lower plates spacing is fixed when working is 8cm, frequency sweep cycle 400s,
Have a rest than 1:1（Ultrasonic 10s, interval 10s）, every piece of vibration plate power is 600W up and down；
Wherein step（5）Described in double frequency combination of frequency be： 40 kHz/ 68kHz、33kHz/22kHz、33 kHz/28
kHz；Most preferably good combination of frequency is 40 kHz/68 kHz；
The design parameter of adverse current impulse ultrasound assistance enzymolysis be 1 ~ 5s of pulse width, 1 ~ 5s of pulse spacing, ultrasonic power density 50 ~
80 W/L；The kHz of supersonic frequency 20, feed liquid passes through ultrasonic probe in the way of circulated in countercurrent.
7. wheat embryo complex enzyme hydrolysis thing according to claim 4, it is characterised in that
Wherein step（4）Described in double frequency frequency sweep ultrasonic upper and lower plates spacing is fixed when working is 10cm, frequency sweep cycle 300s,
Have a rest than 2:1（Ultrasonic 10s, interval 5s）, every piece of vibration plate power is 600W up and down；
Wherein step（4）Described in double frequency combination of frequency be： 40kHz/22kHz、40 kHz/28 kHz、40 kHz/33
kHz、33kHz/22kHz；Most preferably good combination of frequency is 40 kHz/22 kHz；
Wherein step（5）Described multiple frequency ultrasonic pattern is：Three frequencies or double-frequency sequence are ultrasonically treated, and ultrasonic power density 30 ~
150 W/L；The s of ultrasonic pulse working time 10；The s of interpulse period 3；
Wherein step（5）Three described frequencies or the ultrasonically treated ultrasonic frequency of double-frequency sequence are combined as：28/40 kHz、28/
35/40 kHz or 35/40 kHz.
8. wheat embryo complex enzyme hydrolysis thing according to claim 5, it is characterised in that
Wherein step（4）Described multiple frequency ultrasonic pattern is：Three frequencies or double-frequency synchronous are ultrasonically treated, and ultrasonic power density 30 ~
150 W/L；The s of ultrasonic pulse working time 10；The s of interpulse period 3；
Wherein step（4）Three described frequencies or the ultrasonically treated ultrasonic frequency of double-frequency synchronous are combined as： 35/40 kHz、
35/28/40 kHz or 28/40 kHz；
Wherein step（5）Described design parameter is 2 ~ 10s of pulse width, 1 ~ 5s of pulse spacing, ultrasonic power 80W/L, ultrasound
The kHz of frequency 28, feed liquid is in the way of circulated in countercurrent by ultrasonic probe, the min of ultrasonic time 45 ~ 90.
9. the application of the wheat embryo complex enzyme hydrolysis thing according to claim 1-8, wheat embryo complex enzyme hydrolysis thing have compared with
Strong antioxidation activity in vitro, can prepare the food with antioxidation activity, food ingredient or health food.
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| CN113322295A (en) * | 2020-10-20 | 2021-08-31 | 河北肽丰生物科技有限公司 | Preparation method of selenium-rich triticale malt oligosaccharide peptide and product thereof |
| CN117918469A (en) * | 2024-03-04 | 2024-04-26 | 无锡赞匠生物科技有限公司 | Enzymolysis corn rice beverage and preparation method thereof |
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