CN106879476A - A kind of tissue culture method of strawberry - Google Patents
A kind of tissue culture method of strawberry Download PDFInfo
- Publication number
- CN106879476A CN106879476A CN201710296203.3A CN201710296203A CN106879476A CN 106879476 A CN106879476 A CN 106879476A CN 201710296203 A CN201710296203 A CN 201710296203A CN 106879476 A CN106879476 A CN 106879476A
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- China
- Prior art keywords
- strawberry
- tissue culture
- culture method
- aseptic
- seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of tissue culture method of strawberry, the seed of strawberry is soaked in the liquor potassic permanganate that mass concentration is 1 3%, is rinsed with sterilized water afterwards, obtain aseptic seed;The aseptic seed is inoculated into Initial culture base, Initial culture is carried out, aseptic seedling is obtained;Using the cotyledon of the aseptic seedling and hypocotyl as explant, and it is inoculated in subculture medium, carries out squamous subculture, obtains without offspring;Turn to plant in root media without offspring by described, carry out culture of rootage, obtain Strawberry seedlings.Take root efficiency high, survival rate of tissue cultivating and seedling method of the invention is high, can keep the fine quality of plant.
Description
Technical field
The invention belongs to plant tissue culture seedlings-raising technical field, and in particular to a kind of tissue culture method of strawberry.
Background technology
Strawberry nutrition value is abundant, is described as being " fruit queen ", contains abundant vitamin C, vitamin A, vitamin
E, nicotinic acid, vitamin B1, vitamin B2, carrotene, tannic acid, aspartic acid, copper, strawberry amine, pectin, cellulose, leaf
The nutriments such as acid, iron, calcium, ellagic acid and anthocyanidin, especially contained vitamin C, its content 7- all higher than apple, grape
10 times.And contained malic acid, citric acid, vitamin B1, vitamin B2, and the content of carrotene, calcium, phosphorus, iron also compares apple
Really, pears, grape are high 3 to 4 times.
Current strawberry mainly uses seminal propagation and division propagation mode, and reproductive efficiency is low, and easily causes plant quality
Decline.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art, there is provided a kind of tissue culture method of strawberry.
The present invention is realized in the following way：
A kind of tissue culture method of strawberry, the liquor potassic permanganate that mass concentration is 1-3% is soaked in by the seed of strawberry, it
It is rinsed with sterilized water afterwards, obtains aseptic seed;The aseptic seed is inoculated into Initial culture base, be just commissioned to train
Support, obtain aseptic seedling;Using the cotyledon of the aseptic seedling and hypocotyl as explant, and it is inoculated in subculture medium, carries out
Squamous subculture, obtains without offspring;Turn to plant in root media without offspring by described, carry out culture of rootage, obtain Strawberry seedlings.
Preferably, the time being rinsed with the sterilized water is 3-5min.
Preferably, the Initial culture base is the g/L of MS+sucrose 8.
Preferably, the subculture medium is 0.05 mg/L of MS+6- benzyl aminoadenines+heteroauxin 0.02
The g/L of mg/L+sucrose 8.
Preferably, the root media is 1/2MS a great number of elements+MS trace element+MS molysite+MS organic matters+indoles
The g/L of 0.5 mg/L+ sucrose of acetic acid 20.
Preferably, the condition of the Initial culture be 20-25 DEG C, humidity 60-70%, illumination 2000-5000 lx.
Preferably, the condition of the squamous subculture be 20-28 DEG C, humidity 70-80%, illumination 6000-8000 lx.
Preferably, the condition of the culture of rootage is 20-30 DEG C of temperature, humidity 80-90%, illumination 10000-15000
lx。
Advantages and positive effects of the present invention：
1st, take root efficiency high, survival rate of tissue cultivating and seedling method of the invention is high, can keep the fine quality of plant.
2nd, cycle is short of the present invention, easy to operate, suitable extensive nursery.
3rd, with low cost, market prospects of the invention are good, and economic benefit is good.
Specific embodiment
Below by embodiment, the invention will be further described, so that advantages and features of the invention are easier to be managed
Solution, but protection scope of the present invention is not limited to these embodiments.
Embodiment 1
A kind of tissue culture method of strawberry, is soaked in the liquor potassic permanganate that mass concentration is 1%, afterwards by the seed of strawberry
3min is rinsed with sterilized water, aseptic seed is obtained;The aseptic seed is inoculated into Initial culture base, Initial culture base
For the g/L of MS+sucrose 8 carries out Initial culture, 20 DEG C, humidity 60%, illumination 2000lx, obtain aseptic seedling;By the aseptic seedling
Cotyledon and hypocotyl are inoculated in subculture medium as explant, and subculture medium is MS+6- benzyl aminoadenines
0.05 mg/L+0.02 mg/L of heteroauxin+sucrose 8 g/L, 25 DEG C, humidity 70%, the lx of illumination 6000, carry out subculture training
Support, obtain without offspring;Turn to plant in root media without offspring by described, root media is that 1/2MS a great number of elements+MS is micro
Element+MS molysite+MS organic matters+0.5 mg/L+ sucrose of heteroauxin 20 g/L, 28 DEG C, humidity 80%, the lx of illumination 10000, enter
Row culture of rootage, obtains Strawberry seedlings.
Embodiment 2
A kind of tissue culture method of strawberry, is soaked in the liquor potassic permanganate that mass concentration is 3%, afterwards by the seed of strawberry
5min is rinsed with sterilized water, aseptic seed is obtained;The aseptic seed is inoculated into Initial culture base, Initial culture base
For the g/L of MS+sucrose 8 carries out Initial culture, 22 DEG C, humidity 70%, the lx of illumination 5000, obtain aseptic seedling;By the aseptic seedling
Cotyledon and hypocotyl as explant, and be inoculated in subculture medium, subculture medium is MS+6- benzyl aminoadenines
0.05 mg/L+0.02 mg/L of heteroauxin+sucrose 8 g/L, 28 DEG C, humidity 80%, the lx of illumination 8000, carry out subculture
Culture, obtains without offspring;Turn to plant in root media without offspring by described, root media is that 1/2MS a great number of elements+MS is micro-
Secondary element+MS molysite+MS organic matters+0.5 mg/L+ sucrose of heteroauxin 20 g/L, 30 DEG C, humidity 90%, illumination 15000
Lx, carries out culture of rootage, obtains Strawberry seedlings.
Embodiment 3
A kind of tissue culture method of strawberry, is soaked in the liquor potassic permanganate that mass concentration is 2%, afterwards by the seed of strawberry
4min is rinsed with sterilized water, aseptic seed is obtained;The aseptic seed is inoculated into Initial culture base, Initial culture base
For the g/L of MS+sucrose 8 carries out 25 DEG C of Initial culture, humidity 70%, the lx of illumination 3000, aseptic seedling is obtained;By the aseptic seedling
Cotyledon and hypocotyl are inoculated in subculture medium as explant, and subculture medium is MS+6- benzyl aminoadenines
0.05 mg/L+0.02 mg/L of heteroauxin+sucrose 8 g/L, 28 DEG C, humidity 75%, the lx of illumination 7000, carry out subculture
Culture, obtains without offspring;Turn to plant in root media without offspring by described, root media is that 1/2MS a great number of elements+MS is micro-
Secondary element+MS molysite+MS organic matters+0.5 mg/L+ sucrose of heteroauxin 20 g/L, 28 DEG C, humidity 85%, the lx of illumination 12000,
Culture of rootage is carried out, Strawberry seedlings are obtained.
Embodiment 4
A kind of tissue culture method of strawberry, is soaked in the liquor potassic permanganate that mass concentration is 3%, afterwards by the seed of strawberry
5min is rinsed with sterilized water, aseptic seed is obtained;The aseptic seed is inoculated into Initial culture base, Initial culture base
For the g/L of MS+sucrose 8 carries out Initial culture, 25 DEG C, humidity 60%, the lx of illumination 2000, obtain aseptic seedling;By the aseptic seedling
Cotyledon and hypocotyl as explant, and be inoculated in subculture medium, subculture medium is MS+6- benzyl aminoadenines
0.05 mg/L+0.02 mg/L of heteroauxin+sucrose 8 g/L, 28 DEG C, humidity 70%, the lx of illumination 8000, carry out subculture
Culture, obtains without offspring;Turn to plant in root media without offspring by described, root media is that 1/2MS a great number of elements+MS is micro-
Secondary element+MS molysite+MS organic matters+0.5 mg/L+ sucrose of heteroauxin 20 g/L, 30 DEG C, humidity 80%, illumination 15000
Lx, carries out culture of rootage, obtains Strawberry seedlings.
Claims (8)
1. a kind of tissue culture method of strawberry, it is characterised in that the seed of strawberry is soaked in the height that mass concentration is 1-3%
Potassium manganate solution, is rinsed with sterilized water afterwards, obtains aseptic seed;The aseptic seed is inoculated into Initial culture base
In, Initial culture is carried out, obtain aseptic seedling;Using the cotyledon of the aseptic seedling and hypocotyl as explant, and it is inoculated in subculture
In culture medium, squamous subculture is carried out, obtained without offspring;Turn to plant in root media without offspring by described, carry out culture of rootage,
Obtain Strawberry seedlings.
2. the tissue culture method of strawberry according to claim 1, it is characterised in that be rinsed with the sterilized water
Time is 3-5min.
3. the tissue culture method of strawberry according to claim 1, it is characterised in that the Initial culture base be MS+
The g/L of sucrose 8.
4. the tissue culture method of strawberry according to claim 1, it is characterised in that the subculture medium be MS+
0.05 mg/L of the 6- benzyl aminoadenines+g/L of 0.02 mg/L of heteroauxin+sucrose 8.
5. the tissue culture method of strawberry according to claim 1, it is characterised in that the root media is 1/2MS
A great number of elements+MS trace element+MS molysite+MS organic matters+the g/L of 0.5 mg/L+ sucrose of heteroauxin 20.
6. the tissue culture method of strawberry according to claim 1, it is characterised in that the condition of the Initial culture is
20-25 DEG C, humidity 60-70%, illumination 2000-5000 lx.
7. the tissue culture method of strawberry according to claim 1, it is characterised in that the condition of the squamous subculture is
20-28 DEG C, humidity 70-80%, illumination 6000-8000 lx.
8. the tissue culture method of strawberry according to claim 1, it is characterised in that the condition of the culture of rootage is
20-30 DEG C of temperature, humidity 80-90%, illumination 10000-15000 lx.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710296203.3A CN106879476A (en) | 2017-04-28 | 2017-04-28 | A kind of tissue culture method of strawberry |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710296203.3A CN106879476A (en) | 2017-04-28 | 2017-04-28 | A kind of tissue culture method of strawberry |
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| CN106879476A true CN106879476A (en) | 2017-06-23 |
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| CN201710296203.3A Pending CN106879476A (en) | 2017-04-28 | 2017-04-28 | A kind of tissue culture method of strawberry |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107333659A (en) * | 2017-09-15 | 2017-11-10 | 江苏省农业科学院 | A kind of day neutrality Strawberry Plantlets fast breeding culture medium and tissue culture and rapid propagation method |
| CN111328713A (en) * | 2020-03-06 | 2020-06-26 | 贵州大学 | Method for constructing malus asiatica leaf regeneration system |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103858758A (en) * | 2012-12-12 | 2014-06-18 | 东港市草莓研究所 | Strawberry seedling tissue culture rapid propagation technology |
| CN105594596A (en) * | 2016-01-13 | 2016-05-25 | 石磊 | Tissue culture method for strawberry virus-free and rapid propagation for large-scale production |
-
2017
- 2017-04-28 CN CN201710296203.3A patent/CN106879476A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103858758A (en) * | 2012-12-12 | 2014-06-18 | 东港市草莓研究所 | Strawberry seedling tissue culture rapid propagation technology |
| CN105594596A (en) * | 2016-01-13 | 2016-05-25 | 石磊 | Tissue culture method for strawberry virus-free and rapid propagation for large-scale production |
Non-Patent Citations (3)
| Title |
|---|
| 任中翔: "草莓胚状体形态发生的影响因素", 《吉林蔬菜》 * |
| 张经纬等: "草莓离体再生体系的建立", 《草莓研究进展IV》 * |
| 郭月玲等: "我国草莓组织培养生产研究现状及前景", 《浙江农业科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107333659A (en) * | 2017-09-15 | 2017-11-10 | 江苏省农业科学院 | A kind of day neutrality Strawberry Plantlets fast breeding culture medium and tissue culture and rapid propagation method |
| CN107333659B (en) * | 2017-09-15 | 2019-01-22 | 江苏省农业科学院 | A kind of day neutral Strawberry Plantlets fast breeding culture medium and tissue culture and rapid propagation method |
| CN111328713A (en) * | 2020-03-06 | 2020-06-26 | 贵州大学 | Method for constructing malus asiatica leaf regeneration system |
| CN111328713B (en) * | 2020-03-06 | 2022-05-24 | 贵州大学 | Method for constructing malus asiatica leaf regeneration system |
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Application publication date: 20170623 |
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