CN106860491B - Plant extracts and its production and use - Google Patents
Plant extracts and its production and use Download PDFInfo
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- CN106860491B CN106860491B CN201611263369.7A CN201611263369A CN106860491B CN 106860491 B CN106860491 B CN 106860491B CN 201611263369 A CN201611263369 A CN 201611263369A CN 106860491 B CN106860491 B CN 106860491B
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/734—Crataegus (hawthorn)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/87—Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/889—Arecaceae, Palmae or Palmaceae (Palm family), e.g. date or coconut palm or palmetto
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Abstract
The invention discloses Plant Extracts and its production and use.Wherein, preparation method comprises the following steps:Take plant friuit-pit add retort, when being heated to 160 DEG C, constant temperature 1 hour, then temperature rise to 240 DEG C maintain 2 hours;The dry distillation liquid of day part is collected, mixing, 3 days is stood, sedimentation separation, hyaline layer liquid is isolated with siphonage;Activated carbon is added in hyaline layer liquid, filtered fluid is collected and puts in distillation still, 115 DEG C are distilled 2 hours, collect distillate;Distillate is diluted to pH value 2.48 with aqua sterilisa, filters and collects filtered fluid, produce product.Pass through the present invention, solve the problems, such as in correlation technique using caused by chemobiotic drug therapy fungal diseases, bouvardin in plant extracts with it is efficiently antifungal, recurrence rate is lower, with anti-inflammatory activity, antimycotic scope is wide, without hormone, the advantages that without allergin, it is safe to the human body, it is easy to use.
Description
Technical field
The present invention relates to biotechnology and pharmaceutical technology field, in particular it relates to a kind of from natural plants fruit stone
Spray of a variety of antimicrobial components of middle extraction and application thereof.
Background technology
Tinea pedis is the popular name of tinea pedis, and tinea pedis refers to the fungal dermatoses in toe facies palmaris occur, and a kind of extremely common
Fungal infection dermatoses.70%-80% people has tinea pedis in adult, and simply weight is different.Tinea pedis often aggravates in summer,
Winter mitigates, and also someone is not cured throughout the year.
When tinea pedis occurs, fungi growth can produce some smells, after being particularly infected by bacterial, decomposition of protein, can produce
One carrion foul smell, the traditional Chinese medical science are referred to as " smelly river snail " taste.
Cause the fungi of tinea pedis to be dermatophyte, be a kind of fungi for liking living in keratoderma.They have one
Live in soil, referred to as geophilic fungus;There are some to live on animal skin, referred to as griseofulvin;Other
Naturally live in and be referred to as anthropophilic fungus in the fur of people.This three classes fungi can infect the mankind, because route of infection is too many,
Our dermatophytid infection just can usually recur.
Fungi has the characteristics of being difficult to kill, and can muchly be survived in -6 DEG C or so of environment;In 120 DEG C of high temperature
In, will not be dead in 10 minutes;Depart from live body hair, refer to above (toe) first, scurf etc., toxicity can also be kept 1 year with
On.
The medicine for being directed to different types of tinea pedis, the ringworm of the foot, the tinea manuum, jock itch, tinea corporis and mycotic reproductive disease is main
Using the treatment of chemobiotic class medicine, mainly there are propylamine, Thiocarbamate class, azole etc., be able to
Squalene is caused to gather and suppress ergosterol synthesis.But chemobiotic class medicine is easily produced caused by abuse of antibiotics
The problems such as drug resistance, dependence, therefore other medicines are urgently found to treat or prevent fungal diseases.
The content of the invention
The invention provides Plant Extracts and its production and use, at least to solve to use in correlation technique
The problem of caused by chemobiotic drug therapy fungal diseases.
According to an aspect of the invention, there is provided the preparation method of a Plant Extracts, the preparation method is included such as
Lower step:
Take plant friuit-pit to add retort, when being heated to 160 DEG C, constant temperature 1 hour, then temperature rise to 240 DEG C and remain 2 small
When, wherein, plant friuit-pit includes at least one of:Granatum, dark plum core, Pericarppium Armeniacae Amarum, hawthorn shell, grape pip, red bayberry shell, coconut palm
Sub- shell;The dry distillation liquid of day part is collected, mixing, 3 days is stood, sedimentation separation, hyaline layer liquid is isolated with siphonage;Transparent
Activated carbon is added in layer liquid, filtered fluid is collected and puts in distillation still, 115 DEG C are distilled 2 hours, collect distillate;Distillate is used
Aqua sterilisa is diluted to pH value 2.48, filters and collects filtered fluid, produces product.
According to another aspect of the present invention, there is provided a Plant Extracts, the plant extracts is by above-mentioned system
Preparation Method is prepared.
According to another aspect of the present invention, there is provided a kind of above-mentioned plant extracts is preparing treatment Mycophyta tinea disease
Spray in application, by the plant extracts and water according to 1:4 volume ratio mixing, the Mycophyta tinea that obtains medical treatment disease
Spray.
According to another aspect of the present invention, there is provided a kind of above-mentioned plant extracts is preparing treatment or prevention skin
And the application in pudendal pruritus and the spray of bedsore, by the plant extracts and water according to 1:The volume ratio of (5-10) mixes
Close, obtain medical treatment or prevent skin and pudendal pruritus, the spray of bedsore.
According to another aspect of the present invention, there is provided a kind of above-mentioned plant extracts is bacillary and true in preparation treatment
Application in the preparation of bacterium property dysentery, by the plant extracts and water according to 1:The volume ratio mixing of (2-6), is controlled
Treat bacillary and fungoid dysentery preparation.
The spray or preparation of the composition containing plant extracts of the present invention are more comprising being extracted from plant friuit-pit in formula
The complex of kind antimicrobial component, its composition contain phenols 20-45%, organic acid 5-45%, ketone 5-20%, furans 5-
15%, other are micro lipid, alcohols and aldehydes etc..It is extensive to prepare the raw material sources of the complex, can use granatum,
The various plants fruit stones such as dark plum core, Pericarppium Armeniacae Amarum, grape pip, red bayberry core, cocoanut shell, hawthorn shell.
The plant extracts of the present invention is bouvardin mixed liquor, is a variety of antibacterials extracted from natural plants fruit stone
The complex original liquid component composition of composition.The bouvardin is easily absorbed by the skin, and antimycotic and bacterium ability is strong.The Genes For Plant Tolerance
Raw element category acidic materials, pH value can concentrate or be diluted to 2.0-4.0, effectively kill skin surface fungi.
After the dilution that the plant extracts of the present invention passes through different multiples, it can be applied to prepare different types of tinea pedis, pin
Tinea, the tinea manuum, jock itch, the medicine of tinea corporis and mycotic reproductive disease, it is also used for preparing the various itch and cotton-padded mattress of private parts and skin
The medicine of sore, it may also be used for prepare bacillary and fungoid dysentery medicine.The plant extracts has efficiently antifungal, multiple
Hair rate is lower, has an anti-inflammatory activity, and antimycotic scope is wide, without hormone, the advantages that no allergin, safe to the human body, user
Just.
The preparation method technique of the plant extracts of the present invention is simple, is easy to volume production;And relative to being adopted in correlation technique
For ethanol immersion plant friuit-pit and the preparation method of low temperature distillation vacuum concentration, the preparation side of plant extracts of the invention
Method is without using ethanol, and raw material is less, and production process is safer, and cost is also more cheap.
Embodiment
The embodiment of the present invention is in view of the shortcomings of the prior art, there is provided it is a kind of it is easy to use, safe containing plant extracts into
The plant spray divided.
In one embodiment, the preparation method of the plant extracts is using conventional mixing method, using the molten of routine
Solution solvent soln.Take 30 parts of 50 parts of granatum, 80 parts of dark plum core, Pericarppium Armeniacae Amarum addition retorts, when being heated to 160 DEG C, constant temperature 1
Hour, then temperature rises to 240 DEG C and maintained 2 hours;The dry distillation liquid of day part is collected, mixing, 3 days is stood, sedimentation separation, uses rainbow
Suction method isolates hyaline layer liquid;Activated carbon is added in hyaline layer liquid, filtered fluid is collected and puts in distillation still, 115 DEG C of distillations 2
Hour, collect distillate;Distillate is diluted to pH value 2.48 with aqua sterilisa, filters and collects filtered fluid, produce product.
The plant extracts that above-mentioned preparation method obtains can be used for the medicine for preparing prevention and the treatment of following disease:
1st, fungus-caused tinea pedis, the ringworm of the foot, the tinea manuum, jock itch, tinea corporis and mycotic reproductive disease.Preparation method is plant
Extract stoste presses 1 with water:4 volume ratio mixing, pH value 2-5;
2nd, the prevention and treatment of the disease such as the various itch of skin and private parts, bedsore.While sterilization, moreover it is possible to effective
Prevention and the micro-ecological environment of regulation skin surface, prevent the diseases such as various itch, the bedsore of skin and private parts, and enhancing nature is prevented
Imperial function.It is useful and harmless to human body using more convenient, safer.Preparation method is that plant extracts stoste presses 1 with water:(5-
10) volume ratio mixing, pH value 2-4;
3rd, for bacillary and fungoid dysentery intervention medicine.Preparation method is that plant extracts stoste presses 1 with water:
The ratio mixing of (2-6).
The embodiment of the present invention was according to version in 2002《Disinfection technology standard》, pH value, sense are carried out for plant extracts sample
Detection and its stability (bioanalysis) detection are seen, it proves that result is as follows:
1st, microorganism pollution is identified in plant extracts sample
1.1 test material
(1) given the test agent:Plant extracts.
(2) instrument and equipment:Electro-heating standing-temperature cultivator, mold incubator.
1.2 test method
(1) foundation is detected:《Disinfection technology standard》Version " GB15979-2002 Disposable Sanitary Accessories health in 2002
Standard ".
(2) test atmosphere:23 DEG C of temperature;Relative humidity 45%.
1.3 result of the test
Table 1:The microorganism pollution testing result of plant extracts sample stoste
Testing index | Standard value | Measured value |
Total number of bacterial colonies (cfu/ml) | ≤200 | 0 |
Coliform | It must not detect | Do not detect |
Pseudomonas aeruginosa | It must not detect | Do not detect |
Hemolytic streptococcus | It must not detect | Do not detect |
Staphylococcus aureus | It must not detect | Do not detect |
Fungus colony sum (cfu/ml) | ≤100 | 0 |
1.4 conclusion
Total number of bacterial colonies (cfu/m1), coliform, Pseudomonas aeruginosa, the hemolytic hammer of tried plant extracts sample
Bacterium, staphylococcus aureus, fungus colony total (cfu/m1) meet《Disinfection technology standard》The regulation of (version in 2002).
2nd, the bacteriostasis property identification of plant extracts sample is tried
2.1 test material
(1) test strain:Candida albicans (ATCC10231) was cultivated to the 6th generation, by Shandong Center for Disease Control & Prevention
There is provided.
(2) given the test agent:Tried plant extracts sample.
(3) culture medium:Sabouraud's agar, it is standby after pressuresteam sterilization.
2.2 test method
(1) foundation is detected:《Disinfection technology standard》(version in 2002).
(2) test method:24 hours slant cultures of tested bacterium are washed down, phosphate buffer (PSB) is washed down, makes bacterium
Piece is standby.Take that tested sample liquid 4 manages (often 5 milliliters of pipe, test group) and PBS liquid 4 is managed (often 5 milliliters of pipe, control group).Take above-mentioned each bacterium
Suspension, each in above-mentioned developmental tube and control tube respectively to be added dropwise 100 microlitres, uniformly mixing starts timing, effect 2 minutes, 5
Minute, 10 minutes, 20 minutes, 0.5 milliliter of often pipe absorption sample liquid are added in the test tube containing 4.5 milliliters of PBS, fully mixing, fitted
Work as dilution, then take appropriate dilution factor, draw 1 milliliter respectively, four plates are placed in, with cool to 40 DEG C -45 DEG C of sabouraud's agar
15 milliliters of culture medium is poured into, and rotates plate, makes its full and uniform, and flat board is overturn after agar solidification, and 35 DEG C of ± 2 DEG C of cultures 72 are small
When, make the counting of viable bacteria bacterium colony.And calculate bacteriostasis rate:
X=(Nc-Ns)/Nc × 100%;
Wherein, X:Bacteriostasis rate %;Nc:Control sample average colony number;Ns:It is tested sample average clump count.
(3) experiment condition:19℃-21℃.
2.3 result
Tried plant extracts sample acts on Candida albicans 2 minutes, 5 minutes, 10 minutes, 20 minutes average antibacterial
Rate is respectively 98.49%, 98.43%, 98.48%, 99.17%.Concrete outcome is shown in Table 2.
Table 2:The Detection of Stability result of the test of plant extracts sample
2.4 conclusion
Tried plant extracts sample acts on Candida albicans 2 minutes, 5 minutes, 10 minutes, 20 minutes average antibacterial
Rate is respectively 98.49%, 98.43%, 98.48%, 99.17%.
The present invention is described in further details below by embodiment, these embodiments are only used for illustrating the present invention, and
Do not limit the scope of the invention.
Embodiment 1
The woman of the composition containing plant extracts used in embodiments of the invention 1 with preparation spray contain a variety of antibacterials into
Point, no hormone, hurtless measure, it is non-stimulated, have no toxic side effect.The woman can be used for the prevention and treatment of following disease with preparation spray:
Different types of tinea pedis, the ringworm of the foot, the tinea manuum, jock itch, tinea corporis and mycotic reproductive disease.The woman is with the preparation method of preparation spray
For plant extracts stoste and water are pressed into 1:4 volume ratio mixing, is diluted to pH value 2-5;
The present embodiment was according to version in 2002《Disinfection technology standard》, examined for woman with the pH value and sense organ of preparation spray sample
Survey, multiple intact skin irritant test and acute eye irritation test are detected, and it proves that result is as follows:
1st, woman is detected with the pH value and sense organ of preparation spray sample
1.1 test material
(1) given the test agent:Woman is with preparation spray.
(2) instrument and equipment:UB-7 type acidometers.
(3) correction buffer solution:Potassium Hydrogen Phthalate buffer solution (4.00,25 DEG C of pH value), phosphate buffer (pH value
6.86,25 DEG C), from Shanghai Lei Cichuanyi instrument and meters Co., Ltd.
1.2 test method
(1) foundation is detected:《Disinfection technology standard》Version " 2.2.1.4 disinfectants pH value measure " in 2002.
(2) test atmosphere:23 DEG C of temperature;Relative humidity 45%.
(3) operating procedure:Fetch and deliver sample items and survey organoleptic indicator, determination sample stoste pH value.
1.3 result of the test
Table 3:Woman is with the pH value measurement result of preparation spray sample stoste
1.4 conclusion
(1) it is weak yellow liquid to try woman with preparation spray sample.
(2) woman is tried with preparation spray, and the pH value of its stoste is 3.71.
2nd, multiple intact skin irritant test of the woman with preparation spray sample is tried
2.1 materials and animal
(1) given the test agent:Woman is with preparation spray.
(2) animal:Regular grade new zealand rabbit 3, provided by Jinan Xi Lingjiao cultivation Breeding Centers, production licence number:
SCXK (Shandong) 20150001.
(3) environmental condition:Barrier environment animal housing, uses credit number:SYXK (Shandong) 20140010,20 DEG C -25 of temperature
DEG C, relative humidity 50-70%.Rabbit feed is provided by Beijing HFK Bio-Technology Co., Ltd., production licence number:
SCXK (capital) 20140008.
2.2 test method
(1) foundation is detected:《Disinfection technology standard》(version in 2002).
(2) sample preparation:Tested using plant extracts stoste.
(3) test procedure:The day before yesterday is tested, experiment rabbit back backbone both sides hair is cut, unhairing scope left and right each about 3
3 centimetres of cm x.Tested using Self-control method, take 0.5 milliliter of given the test agent, be coated on the skin that right side is exposed,
Left side exposed skin is as control.After every experimental rabbit is smeared 4 hours, the skin for smearing position is cleaned with warm water, removes residual
Thing;It is continuous to smear 14 days.24h observed and recorded the dermoreaction for smearing position after each smear respectively, by version in 2002《Sterilization
Technical specification》Skin wound repair standards of grading carry out dermoreaction integration and stimulus intensity evaluation.
2.3 result of the test
After removing tested material, zoopery position does not occur the anomalous variations such as erythema, oedema, the daily every animal of the sample
Integral mean value is 0.
Table 4:Woman is with the multiple Skin Irritation Test result of preparation spray sample
2.4 conclusion (of pressure testing):
According to version in 2002《Disinfection technology standard》Skin wound repair scores and irritated strength grading standard, plant spray
Sample stoste carries out multiple intact skin irritant test with experimental rabbit and belongs to nonirritant to skin.
3rd, acute eye irritation test of the woman with preparation spray is tried
3.1 materials and animal
(1) given the test agent:Woman is with preparation spray.
(2) animal subject:Regular grade new zealand rabbit 3, provided by Jinan Xi Lingjiao cultivation Breeding Centers, production licence
Number:SCXK (Shandong) 20150001.
(3) environmental condition:Barrier environment animal housing, uses credit number:SYXK (Shandong) 20140010,2-25 DEG C of temperature,
Relative humidity 50-70%.Rabbit feed is provided by Beijing HFK Bio-Technology Co., Ltd., production licence number:SCXK
(capital) 20140008.
3.2 test method
(1) foundation is detected:《Disinfection technology standard》Version " 2.3.4 acute oculars stimulation test " in 2002.
(2) sample preparation:Tested using sample stoste as tested material.
(3) test procedure:0.1 milliliter of tested material is drawn, instillation experimental rabbit side eye conjunctiva is intracapsular, and another branch hole is with physiology
Salt solution is as normal control.After dripping tested material, normal saline flushing is used after eye is passively closed 4 seconds, 30 seconds, 1 is small after eye drip
When, visually observe within 24 hours, 48 hours, 72 hours experiment rabbit conjunctival, iris close angle film damage and recovery situation, and to thorn
Sharp reaction is scored, and calculate respectively every animal 24 hours, 48 hours, the corneal injury of 72 hours, iris infringement, knot
The congested average score with the aspect of chemosis four of film, and judge that stimulation of the tested material to eyes is strong by Eye irritation grade scale
Degree.
3.3 result of the test
Average score difference of 3 experimental rabbits at corneal injury, iris infringement, conjunctival congestion and the aspect of chemosis four
For:Corneal injury 0 divides;Iris damages 0 point;Conjunctival congestion 1.67 divides;Chemosis 1.33 is divided, and all dynamic within 7 days
The Eye irritation reaction of thing recovers completely.
Table 5:Woman is reacted experimental rabbit Eye irritation with preparation spray sample to score
Note:Average score means 24 hours, 48 hours, 72 hours score sums divided by observing time hop counts 3
3.4 conclusion
According to version in 2002《Disinfection technology standard》Skin wound repair scores and stimulus intensity grade scale, plant spray
Sample stoste carries out acute eye irritation test with experimental rabbit and belongs to nonirritant.
Embodiment 2
The plant extracts used in embodiments of the invention 2 using the compound of a variety of antimicrobial components as main component,
Without hormone, hurtless measure, it is non-stimulated, have no toxic side effect.The plant extracts can be used for various itch, the cotton-padded mattress for preparing skin and private parts
The medicine of prevention and the treatment of the diseases such as sore.The medicine being prepared is while sterilization, moreover it is possible to effective prevention and regulation skin
The micro-ecological environment on skin surface, prevent the diseases such as various itch, the bedsore of skin and private parts, strengthen natural defense function;Use
It is more convenient, safer, it is useful and harmless to human body.Prepare the medicine as diseases such as the various itch for skin and private parts, bedsores
The method of thing:Plant extracts stoste and water are pressed 1:The volume ratio mixing of (5-10), pH value 2-4.
In the present embodiment according to version in 2002《Disinfection technology standard》, for the various itch for skin and private parts,
PH value and the sense organ detection of the sample of the plant spray of the diseases such as bedsore, its bacteriostasis property, vaginal mucomembranous irritant test are examined
Survey, it proves that result is as follows:
1st, the bacteriostasis property detection of plant spray sample.
1.1 test material
(1) test strain:Candida albicans (ATCC10231) was cultivated to the 6th generation, by Shandong Center for Disease Control & Prevention
There is provided.
(2) given the test agent:Plant spray for diseases such as the various itch of skin and private parts, bedsores.
(3) examination carrier is received:Pieces of cloth.
(4) culture medium:Sabouraud's agar, it is standby after pressuresteam sterilization.
1.2 test method
(1) foundation is detected:《Disinfection technology standard》(version in 2002).
(2) operating procedure:
(1) experimental condition:19℃-21℃.
(2) 24 hours slant cultures of tested bacterium are washed down, trypticase soy broth (Trypticase Soy
Broth, referred to as TSB) the outstanding bacterium solution of configuration, it is standby to make bacterium piece.Separately sampled product 5g, add bacterium piece 1, start timing, effect
To 2 minutes, 5 minutes, 10 minutes, 20 minutes, take out, 5 milliliters of addition, in dilution test tube, fully mix, suitably dilute conduct
Test group.Take bacterium piece 1 to be directly added into 5 milliliters of dilution test tubes, fully mix, appropriate dilution is as a control group.Inhale respectively
1 milliliter is taken, is placed in two plates, is poured into cool to 40 DEG C -45 DEG C of 15 milliliters of nutrient agar, plate is rotated, makes
Its is full and uniform, and flat board is overturn after agar solidification, and 35 DEG C ± 2 DEG C are cultivated 72 hours, makees the counting of viable bacteria bacterium colony.And calculate antibacterial
Rate:
X=(Nc-Ns)/Nc × 100%
Wherein, X:Bacteriostasis rate %;Nc:Control sample average colony number;Ns:It is tested sample average clump count.
1.3 result of the test
Tried plant spray sample acts on Candida albicans 2 minutes, 5 minutes, 10 minutes, the average bacteriostasis rate of 20 minutes
Respectively 90.32%, 92.48%, 97.74%, 97.51%.Concrete outcome is shown in Table 6.
Table 6:The bacteriostasis property test result of plant spray
1.4 conclusion
Tried plant spray acts on Candida albicans 2 minutes, 5 minutes, 10 minutes, the average bacteriostasis rate difference of 20 minutes
For 88.49%, 94.70%, 97.48%, 99.17%.
2nd, the sample bacteriostasis property identification of plant spray
2.1 test material
(1) test strain:Staphylococcus aureus (ATCC6538) culture to the 6th generation, Escherichia coli (8099) are cultivated extremely
In 6th generation, provided by Shandong Center for Disease Control & Prevention.
(2) given the test agent:Plant spray for diseases such as the various itch of skin and private parts, bedsores.
(3) culture medium:Nutrient agar, it is standby after pressuresteam sterilization.
2.2 test method
(1) foundation is detected:《Disinfection technology standard》(version in 2002).
(2) operating procedure:
Tested bacterium 24h slant cultures are washed down with PBS, the bacteria suspension that suitable concentration is made is standby.Tested sample liquid 4 is taken to manage
(often pipe 5m1, test group) and PBS liquid 4 are managed (often pipe 5ml, control group).The suspension of above-mentioned each bacterium is taken, respectively in above-mentioned developmental tube
100 microlitres are added dropwise with each in control tube, uniformly mixing, starts timing, acts on 2 minutes, 5 minutes, 10 minutes, 20 minutes, often manage
Sample liquid 0.5 is drawn like adding in the test tube containing 4.5 milliliters of PBS, is fully mixed, makees appropriate dilution, then takes appropriate dilution factor,
1 milliliter is drawn respectively, two plates is placed in, is poured into cool to 40 DEG C -45 DEG C 4 of 15 milliliters of nutrient agar, is rotated
Plate, make its full and uniform, flat board is overturn after agar solidification, 35 DEG C ± 2 DEG C are cultivated 48 hours, make the counting of viable bacteria bacterium colony.And count
Calculate bacteriostasis rate:
X=(Nc-Ns)/Nc × 100%
Wherein, X:Bacteriostasis rate %;Nc:Control sample average colony number;Ns:It is tested sample average clump count.
(3) experimental condition:19℃-21℃.
2.3 result of the test
Tried plant spray acts on staphylococcus aureus 2 minutes, 5 minutes, 10 minutes, the average bacteriostasis rate of 20 minutes
Respectively 100%, 100%, 100%, 100%;Escherichia coli are acted on 2 minutes, 5 minutes, 10 minutes, the average suppression of 20 minutes
Bacterium rate is respectively 100%, 100%, 100%, 100%.Concrete outcome is shown in Table 7 and table 8.
Table 7:Plant spray sample is to staphylococcus aureus bacteriostasis property testing experiment
Table 8:Bacteriostasis property testing experiment result of the plant spray sample to Escherichia coli
2.4 conclusion
Tried plant spray acts on staphylococcus aureus 2 minutes, 5 minutes, 10 minutes, the average bacteriostasis rate of 20 minutes
Respectively 100%, 100%, 100%, 100%;Escherichia coli are acted on 2 minutes, 5 minutes, 10 minutes, the average suppression of 20 minutes
Bacterium rate is respectively 100%, 100%, 100%, 100%.
3rd, the vaginal mucomembranous irritant test of plant spray is tried
3.1 materials and animal
(1) given the test agent:Plant spray for diseases such as the various itch of skin and private parts, bedsores.
(2) animal:Regular grade new zealand rabbit 6, provided by Jinan Xi Lingjiao cultivation Breeding Centers, production licence number:
SCXK (Shandong) 20150001.
(3) environmental condition:Barrier environment animal housing, uses credit number:SYXK (Shandong) 20140010,20 DEG C -25 of temperature
DEG C, relative humidity 50-70%.Rabbit feed is provided by Beijing HFK Bio-Technology Co., Ltd., production licence number:
SCXK (capital) 20140008.
3.2 test method
(1) foundation is detected:《Disinfection technology standard》Version " 2.3.5 items vaginal mucomembranous irritant test " in 2002.
(2) sample preparation:Tested using sample stoste.
(3) test procedure:6 experimental rabbits are randomly divided into experimental group and control group, every group 3.Checked before experiment each dynamic
Thing introitus whether there is secretion, hyperemia and other degree of impairments, and blunt nosed flexible pipe (conduit) is connected with 5mL syringe.Experiment
Rabbit faces upward fixation, exposure perineum and introitus, by conduit with gently inserting 4-5 centimetres of vagina after being moistened by test solution, and with noting
Emitter is slowly injected into 2 milliliters by test solution, gently extracts conduit out.Control group does same processing with physiological saline.Adopted after 24 hours
Animal is put to death with aeroembolism method, takes out complete vagina, and it is longitudinally slit, and whether visually observe has the naked eyes lesion such as bleeding, oedema,
The both ends and middle three positions for choosing vagina carry out routine paraffin wax embedding and section, hematoxylin eosin staining method
After (Hematoxylin-Eosin staining, referred to as HE) dyeing, pathological study is carried out.According to pathologic finding knot
Fruit, the stimulate the reaction of vagina mucosa is scored, calculate the average integral of the stimulate the reaction of vagina mucosa, tried to achieve stimulation and refer to
Number, carry out stimulus intensity classification.
3.3 result of the test
Experimental group and control animals vagina are showed no the obvious naked eyes pathological change such as hyperemia, oedema, histopathology inspection
Look into and be shown in Table 9.Test group vaginal mucosa irritation reaction average integral is 1.89, and control group average integral is 1.0, vaginal mucosa irritation
Index is 0.89.
Table 9:The vaginal mucosa irritation reaction scoring of plant spray
3.4 conclusion (of pressure testing)
According to version in 2002《Disinfection technology standard》Vagina mucosa irritates strength grading standard, plant spray sample stoste pair
Vagina mucosa belongs to nonirritant.
Embodiment 3
The plant extracts used in embodiments of the invention 3 using the compound of a variety of antimicrobial components as main component,
Without hormone, hurtless measure, it is non-stimulated, have no toxic side effect.The plant extracts can be used for preparing bacillary and fungoid dysentery medicine
Thing.Prepare for the bacillary and medicine of fungoid dysentery method:Plant extracts stoste and water are pressed 1:The volume of (2-6)
Ratio mixes.The galenical being prepared is while sterilization, moreover it is possible to and it is effective to improve vivo environment and micro-ecological environment, increase
Strong natural defense function.It is useful and harmless to human body using more convenient, safer.
In the present embodiment, according to version in 2002《Disinfection technology standard》, pH value for its sample, sense organ detection and
Sample microbial pollution identification, its bacteriostasis property are detected, and it proves that result is as follows:
1st, the pH value of galenical sample and sense organ detection
1.1 test material
(1) given the test agent:For bacillary and fungoid dysentery galenical.
(2) instrument and equipment:UB-7 type acidometers.
(3) correction buffer solution:Potassium Hydrogen Phthalate buffer solution (4.00,25 DEG C of pH value), phosphate buffer (pH value
6.86,25 DEG C), from Shanghai Lei Cichuanyi instrument and meters Co., Ltd.
1.2 test method
(1) detection according to:《Disinfection technology standard》Version " 2.2.1.4 disinfectants pH value measure " in 2002.
(2) test atmosphere:23 DEG C of temperature;Relative humidity 45%.
(3) operating procedure:Fetch and deliver sample items and survey organoleptic indicator, determination sample stoste pH value.
1.3 result of the test
Table 10:The pH value measurement result of galenical sample stoste
1.4 conclusion
(1) it is weak yellow liquid to try galenical sample.
(2) pH value for trying galenical sample stoste is 2.46.
2nd, galenical sample microbial pollution identification
2.1 test material
(1) test specimen:Galenical.
(2) electro-heating standing-temperature cultivator.
(3) mold incubator.
2.2 test method
(1) foundation is detected:《Disinfection technology standard》Version " GB15979-2002 Disposable Sanitary Accessories health in 2002
Standard ".
2.3 result of the test
Table 11:Galenical sample microbial pollution detection result
Testing index | Standard value | Measured value |
Total number of bacterial colonies (cfu/ml) | It must not detect | Do not detect |
Coliform | It must not detect | Do not detect |
Pseudomonas aeruginosa | It must not detect | Do not detect |
Hemolytic streptococcus | It must not detect | Do not detect |
Staphylococcus aureus | It must not detect | Do not detect |
Fungus colony sum (cfu/ml) | ≤100 | 0 |
2.4 conclusion
Total number of bacterial colonies (cfu/m1), coliform, Pseudomonas aeruginosa, hemolytic streptococcus, the gold of tried galenical
Staphylococcus aureus, fungus colony total (cfu/m1) meet《Disinfection technology standard》The regulation of (version in 2002).
3rd, galenical sample bacteriostasis property is identified
3.1 test material
(1) test strain:Staphylococcus aureus (ATCC6538) culture to the 6th generation, Escherichia coli (8099) are cultivated extremely
In 6th generation, provided by Shandong Center for Disease Control & Prevention.
(2) given the test agent:Galenical.
(3) culture medium:Nutrient agar, it is standby after pressuresteam sterilization.
3.2 test method
(1) foundation is detected:《Disinfection technology standard》(version in 2002).
(2) operating procedure:
Tested bacterium 24h slant cultures are washed down with PBS, the bacteria suspension that suitable concentration is made is standby.Tested sample liquid 4 is taken to manage
(often pipe 5m1, test group) and PBS liquid 4 are managed (often pipe 5ml, control group).The suspension of above-mentioned each bacterium is taken, respectively in above-mentioned developmental tube
100 microlitres are added dropwise with each in control tube, uniformly mixing, starts timing, acts on 2 minutes, 5 minutes, 10 minutes, 20 minutes, often manage
Sample liquid 0.5 is drawn like adding in the test tube containing 4.5 milliliters of PBS, is fully mixed, makees appropriate dilution, then takes appropriate dilution factor,
1 milliliter is drawn respectively, two plates is placed in, is poured into cool to 40 DEG C -45 DEG C 4 of 15 milliliters of nutrient agar, is rotated
Plate, make its full and uniform, flat board is overturn after agar solidification, 35 DEG C ± 2 DEG C are cultivated 48 hours, make the counting of viable bacteria bacterium colony.And count
Calculate bacteriostasis rate:
X=(Nc-Ns)/Nc × 100%
Wherein, X:Bacteriostasis rate %;Nc:Control sample average colony number;Ns:It is tested sample average clump count.
(3) experimental condition:19℃-21℃.
3.3 result of the test
Tried galenical acts on staphylococcus aureus 2 minutes, 5 minutes, 10 minutes, the average bacteriostasis rate of 20 minutes
Respectively 100%, 100%, 100%, 100%;Escherichia coli are acted on 2 minutes, 5 minutes, 10 minutes, the average suppression of 20 minutes
Bacterium rate is respectively 100%, 100%, 100%, 100%.Concrete outcome is shown in Table 12 and table 13.
Table 12:Galenical sample is to staphylococcus aureus bacteriostasis property test result
Table 13:Bacteriostasis property test result of the galenical sample to Escherichia coli
3.4 conclusion
Tried galenical acts on staphylococcus aureus 2 minutes, 5 minutes, 10 minutes, the average bacteriostasis rate of 20 minutes
Respectively 100%, 100%, 100%, 100%;Escherichia coli are acted on 2 minutes, 5 minutes, 10 minutes, the average suppression of 20 minutes
Bacterium rate is respectively 100%, 100%, 100%, 100%.
4th, the acute eye irritation test of galenical is tried
4.1 materials and animal
(1) given the test agent:Galenical, weak yellow liquid.
(2) animal subject:Regular grade new zealand rabbit 3, provided by Jinan Xi Lingjiao cultivation Breeding Centers, production licence
Number:SCXK (Shandong) 20150001.
(3) environmental condition:Barrier environment animal housing, uses credit number:SYXK (Shandong) 20140010,2-25 DEG C of temperature,
Relative humidity 50-70%.Rabbit feed is provided by Beijing HFK Bio-Technology Co., Ltd., production licence number:SCXK
(capital) 20140008.
4.2 test method
(1) foundation is detected:《Disinfection technology standard》Version " 2.3.4 acute oculars stimulation test " in 2002.
(2) sample preparation:Tested using sample stoste as tested material.
(3) test procedure:0.1 milliliter of tested material is drawn, instillation experimental rabbit side eye conjunctiva is intracapsular, and another branch hole is with physiology
Salt solution is as normal control.After dripping tested material, normal saline flushing is used after eye is passively closed 4 seconds, 30 seconds, 1 is small after eye drip
When, visually observe within 24 hours, 48 hours, 72 hours experiment rabbit conjunctival, iris close angle film damage and recovery situation, and to thorn
Sharp reaction is scored, and calculate respectively every animal 24 hours, 48 hours, the corneal injury of 72 hours, iris infringement, knot
The congested average score with the aspect of chemosis four of film, and irritate grade scale by eye and judge that stimulation of the tested material to eyes is strong
Degree.
4.3 result of the test
Average score difference of 3 experimental rabbits at corneal injury, iris infringement, conjunctival congestion and the aspect of chemosis four
For:Corneal injury 0 divides;Iris damages 0 point;Conjunctival congestion 1.67 divides;Chemosis 1.33 is divided, and all dynamic within 7 days
The Eye irritation reaction of thing recovers completely.
Table 14:Galenical, which is reacted experimental rabbit Eye irritation, to score
Note:Average score means 24 hours, 48 hours, 72 hours score sums divided by observing time hop counts 3.
4.4 conclusion
According to version in 2002《Disinfection technology standard》Skin wound repair scores and stimulus intensity grade scale, galenical
Stoste carries out acute eye irritation test with experimental rabbit and belongs to nonirritant.
In summary, a variety of anti-fungal compositions extracted from plant friuit-pit are included in plant extract composition formula of the invention
Complex, the plant extracts is acidic materials, pH value 2.0-4.0, with it is efficiently antifungal, recurrence rate is lower, has and resists
Scorching activity, antimycotic scope is wide, without hormone, safe to the human body the advantages that no allergin, easy to use.The plant extracts
Available for the medicine for preparing fungus-caused tinea pedis, the ringworm of the foot, the tinea manuum, jock itch, tinea corporis and mycotic reproductive disease;It is also used for making
The medicine of the various itch and bedsore of standby private parts and skin;It can also be used to prepare bacillary and fungoid dysentery medicine.This
Invention plant extracts preparation method cost is cheap, safe and convenient to use, and it is promoted and exploitation prospect is wide.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.Within the spirit and principles of the invention, that is made any repaiies
Change, equivalent substitution, improvement etc., should be included in the scope of the protection.
Claims (5)
1. the preparation method of a Plant Extracts, it is characterised in that the preparation method comprises the following steps:
30 parts of 50 parts of granatum, 80 parts of dark plum core, Pericarppium Armeniacae Amarum addition retorts are taken, when being heated to 160 DEG C, constant temperature 1 hour, then
Temperature rises to 240 DEG C and maintained 2 hours;
The dry distillation liquid of day part is collected, mixing, 3 days is stood, sedimentation separation, hyaline layer liquid is isolated with siphonage;
Activated carbon is added in hyaline layer liquid, filtered fluid is collected and puts in distillation still, 115 DEG C are distilled 2 hours, collect distillate;
Distillate is diluted to pH value 2.48 with aqua sterilisa, filters and collects filtered fluid, produce product.
A 2. Plant Extracts, it is characterised in that the plant extracts as described in claim 1 preparation method prepare and
Come.
3. application of the plant extracts in the spray for preparing treatment Mycophyta tinea disease described in a kind of claim 2, its feature
It is, by the plant extracts and water according to 1:4 volume ratio mixing, the spray of the Mycophyta tinea that obtains medical treatment disease.
4. the plant extracts described in a kind of claim 2 is preparing treatment or prevention skin and pudendal pruritus and the spray of bedsore
In application, it is characterised in that by the plant extracts and water according to 1:(5-10) volume ratio mixing, obtain medical treatment or
Prevent skin and pudendal pruritus, the spray of bedsore.
A kind of 5. plant extracts the answering in the bacillary and preparation of fungoid dysentery is treated in preparation described in claim 2
With, it is characterised in that by the plant extracts and water according to 1:(2-6) volume ratio mixing, obtain medical treatment it is bacillary and
The preparation of fungoid dysentery.
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山楂核溜油的研制和应用;铁汉等;《中华医学杂志》;19781231;第58卷(第6期);75 * |
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