CN106841013A - A kind of method that microscopic organism is distinguished based on Quantum Correlation imaging technique - Google Patents
A kind of method that microscopic organism is distinguished based on Quantum Correlation imaging technique Download PDFInfo
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- CN106841013A CN106841013A CN201710156195.2A CN201710156195A CN106841013A CN 106841013 A CN106841013 A CN 106841013A CN 201710156195 A CN201710156195 A CN 201710156195A CN 106841013 A CN106841013 A CN 106841013A
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- 238000003384 imaging method Methods 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 30
- 238000005259 measurement Methods 0.000 claims abstract description 13
- 238000006243 chemical reaction Methods 0.000 claims abstract description 6
- 239000013078 crystal Substances 0.000 claims description 10
- 238000005086 pumping Methods 0.000 claims description 7
- 230000005540 biological transmission Effects 0.000 claims description 4
- 238000011835 investigation Methods 0.000 claims 1
- 238000010586 diagram Methods 0.000 description 5
- 238000001093 holography Methods 0.000 description 4
- 230000010287 polarization Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 230000005855 radiation Effects 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N argon Substances [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000012634 optical imaging Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G01N15/1433—Signal processing using image recognition
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Abstract
The invention discloses a kind of method that microscopic organism is distinguished based on Quantum Correlation imaging technique, the method is that a series of image of different microscopic organisms is projected on a holofilm by multiple exposure holographic technique, then microscopic organism to be measured and the hologram for preparing are individually positioned in the entangled photon pairs light path produced by parametric down conversion process, microscopic organism wherein to be measured is placed in the light path of leisure light, hologram is placed in the light path of flashlight, using common intensity detector is to the leisure light by object under test and passes through hologram and be diffracted into by the flashlight on different directions carries out coincidence measurement to distinguish tested organism.General intensity detector, and flashlight is used not to be contacted with imaging object due to during, the method can realize high-resolution, low cost imaging.
Description
Technical field
The present invention relates to a kind of method that microscopic organism is distinguished based on Quantum Correlation imaging technique, belong to quantum optices and
The interleaving techniques field of imaging technique.
Background technology
Micro-imaging is a kind of means observed and recognize small items, and classical optics imaging technique is set up in electromagnetic wave
On certainty theory model (geometric optics, wave optics) and classical theory of information basis, by the average light for recording radiation field
The image information of (or position phase) distributed acquisition target by force.The imaging detection sensitivity of classical optics imaging technique cannot surmount detection
The quantum noise limit of system, its Optical Resolution of Imaging System cannot surmount its resolution diffraction limit, and its scanning imagery speed
Rate cannot surmount the nyquist sampling limit of classical theory of information.
Believed so as to obtain the image of target by recording the light distribution of radiation field relative in traditional optical imaging technique
The method of breath, quantum imaging is then the image that object is obtained by using the quantum fluctuation of, control (or simulation) radiation field.It is beautiful
Shi Yanhua groups of University of Maryland of state experimentally realize relevance imaging first in nineteen ninety-five, using wavelength 351.1nm, laser
The argon ion laser of spot diameter 2mm produces tangling for a pair of parametric down conversions as pump light source pumping II type bbo crystals
Photon pair, referred to as lie fallow light and flashlight, and by a polarization beam apparatus, flashlight is divided into two-way with reference light, in reference light
Microscopic organism to be measured is placed in light path, by record two coincidence countings of light path, can obtain object clearly as.Although letter
Number light do not have and contacted with microscopic organism to be measured, due to flashlight and leisure just highlights correlations, can at flashlight end
The characteristic information of microscopic organism to be measured is presented.Quantum Correlation imaging technique can reach or break through diffraction limit, by symbol
Closing measurement effectively can realize the measurement of Resolving by compression noise.But the technology needs high-precision
CCD camera so that distinguish that cost is raised.
The content of the invention
In order to solve the above problems, the present invention provides a kind of side that microscopic organism is distinguished based on Quantum Correlation imaging technique
Method, the method can rapidly and accurately identify microscopic organism on the premise of low cost.
It is involved in the present invention it is a kind of microscopic organism is distinguished based on Quantum Correlation imaging technique method the step of be：
Step one, one multiple exposure holofilm of acquisition
A kind of microscopic organism of standard is placed in hologram photoplate front, the microbiological of standard is irradiated with laser beam
Body, the light beam after transmission is projected on a holofilm jointly with the laser beam of another Shu Zuowei reference beams；Repeat this
One process, different microscopic organisms is projected on same holofilm, the reference light that different microscopic organisms is used
Shu Fangxiang is different, i.e., different reference light directions is unique to should microscopic organism；Egative film development, fixing treatment are divided
Secondary exposed holographic egative film；
Step 2
Entangled photon pairs light path is built, including is irradiated on bbo crystal with a branch of pumping laser and is produced a pair of lower turn of parameters
The entangled photon pairs for changing, are referred to as leisure light and flashlight；Microscopic organism to be measured is placed in the light path of leisure light, by several times
Exposed holographic egative film is put into the light path of flashlight, to the leisure light by microscopic organism to be measured and holographic by multiple exposure
Egative film is simultaneously diffracted into after the flashlight on different directions is utilized respectively common intensity detector measurement, and measurement result is accorded with
Total number can tell microscopic organism to be measured；Microscopic organism to be measured need to be project to it is micro- on multiple exposure holofilm
See one kind of organism.
Further, the microscopic organism for being projected on the multiple exposure holofilm is two or more.
Further, in the light path of the leisure light, between microscopic organism to be measured and bbo crystal placement can adjust filtering
Piece.
Further, in the light path of the flashlight it is, general in the light path of multiple exposure holofilm and each flashlight
Adjustable filter plate is placed between logical intensity detector.
Compared with prior art, multiple exposure holographic technique and Quantum Correlation imaging technique are innovatively combined and answered by the present invention
For distinguishing for microscopic organism, general intensity detector, and flashlight is used not to be connect with imaging object due to during
Touch, realize on the premise of low cost, quick, high-resolution and highly sensitive measurement.
Brief description of the drawings
Fig. 1 is the microscopic organism A multiple exposures holography process schematic of standard；
Fig. 2 is the microscopic organism B multiple exposures holography process schematic of standard；
Fig. 3 is the microscopic organism A holofilm read-out principle schematic diagrames of standard；
Fig. 4 is the microscopic organism B holofilm read-out principle schematic diagrames of standard；
Fig. 5 is Quantum Correlation image-forming principle schematic diagram；
Fig. 6 is the schematic diagram that microscopic organism is distinguished based on multiple exposure holographic imaging and Quantum Correlation imaging technique；
Wherein 1 is laser beam, and 2 is microscopic organism A, and 3 is reference beam a, and 4 is holofilm a, and 5 is microscopic organism
B, 6 is reference beam b, and 7 is holofilm b, and 8 is output light a, and 9 is output light b, and 10 is pumping laser, and 11 is bbo crystal, 12
It is leisure light, 13 is flashlight, and 14 is detector, and 15 is CCD camera, and 16 is coincidence counting device, and 17 is microscopic organism to be measured
A, 18 is the imaging of microscopic organism a to be measured, and 19 is adjustable filter plate, and 20 is microscopic organism b to be measured, and 21 is light path a, 22
It is light path b.
Specific embodiment
The present invention is described in detail below in conjunction with the accompanying drawings, and content described herein is merely to illustrate and explains this hair
It is bright, it is not intended to limit the present invention.
The first step：Acquisition has a holofilm of numerous microscopic organism projections.It is specific as follows：
The microscopic organism A2 of standard is irradiated with laser beam 1 first, as shown in figure 1, the microscopic organism A2 of standard is put
In hologram photoplate front, the microscopic organism A2 of the vertical irradiation standard of laser beam 1 projected the microscopic organism of standard
The laser beam of A2 forms the object beam of diffusion formula, is mapped to another laser beam as reference beam a 3 on egative film, and object beam is folded
Plus interference is produced, the position phase and amplitude of each point on object light wave are converted into the intensity being spatially varying, so that using interference
The full detail of object light wave is recorded at interfringe contrast and interval.The object beam of transmission is common with reference beam a 3
Project on holofilm, formation records the holofilm a 4 of the interference fringe of the microscopic organism A2 of standard.We utilize
Same process projects on same holofilm the microscopic organism B 5 of standard, is not both uniquely and uses reference light
Beam b 6, the direction of its reference light and the reference light of the microscopic organism A2 of standard are that the direction of reference beam a 3 is different, such as Fig. 2
It is shown.Diverse microcosmic bioagent reference light direction is different, i.e., different reference light directions is unique to should microscopic organism.So
The multiple exposure holofilm b that the microscopic organism B5 of the microscopic organism A2 and standard that possess standard that just completed is projected
7.Multiple different images can also be recorded on same egative film by multiexposure, multiple exposure, and can be shown respectively without interfering with each other
Out, require that the direction of reference beam can not be identical during.This process can repeatedly, therefore this method can be with
Numerous microscopic organisms is projected on a holofilm.Egative film is again by the program treatment such as development, fixing afterwards.This shows
It is intended to and schematic diagram below is illustrated as a example by distinguishing microscopic organism A2 and microscopic organism B5 to the technology, but
It is not limited thereto, illustrates hereby.
Second step：Entangled photon pairs light path is built, is irradiated on bbo crystal with a branch of pumping laser under producing a pair of parameters
The entangled photon pairs of conversion, are referred to as leisure light and flashlight.To the leisure light by microscopic organism to be measured and by complete
Breath egative film is simultaneously diffracted into the flashlight on different directions and is utilized respectively detector and carry out coincidence measurement.It is specific as follows：
The principle schematic of Quantum Correlation imaging is incited somebody to action as shown in figure 5, being irradiated on bbo crystal 11 with a branch of pumping laser 10
A pair of entangled photon pairs of parametric down conversion are produced, referred to as lie fallow light 12 and flashlight 13.By microscopic organism a 17 to be measured
It is placed in the light path of leisure light 12, the leisure light 12 to passing through is measured with detector 14, detector 14 is visited for general intensity
Survey device, do not need high-resolution.Spacescan measurement is carried out to flashlight 13 with high-resolution CCD camera 15 simultaneously, then
Using coincidence counting device 16 pairs, both carry out coincidence counting, you can the imaging 18 of high-resolution microscopic organism a to be measured is presented.
The present invention is the side based on multiple exposure holographic imaging and Quantum Correlation imaging technique rapid identification microscopic organism
Method.As shown in fig. 6, be irradiated on bbo crystal 11 a pair of entangled photons of parametric down conversion will be produced with a branch of pumping laser 10
Right, referred to as lie fallow light 12 and flashlight 13.Microscopic organism b 20 to be measured is placed in the leisure light path of light 12, and to logical
The leisure light 12 crossed is measured with detector 14, and detector 14 is general intensity detector, does not need high-resolution.It is to be measured microcosmic
Organism b 20 is the one kind for projecting to the microscopic organism on holofilm.Simultaneously early stage is placed in the light path of flashlight 13
The multiple exposure holofilm b 7 of preparation, after flashlight 13 is diffracted on different directions after by holofilm b 7,
During multiple exposure holography use two with reference to respectively being placed on i.e. reference beam a3 on light direction and reference beam b6 directions
One detector 14, detector 14 is general intensity detector.Its principle is as shown in Figure 3 and Figure 4.Irradiated when with monochromatic light beamlet
Microscopic organism A2, allows the light beam of transmission to project on the multiple exposure holofilm b7 for preparing, and now passes through by diffraction
The direction of the output light a8 of holofilm b7 can be reference light with the reference light of the microscopic organism A2 holofilms of the standard of making
Beam a3 directions are consistent, as shown in Figure 3.Similarly, monochromatic light beamlet irradiation microscopic organism B5, by multiple exposure holofilm
The direction of the output light b9 after b7 is reference beam b6 directions with the reference light of the microscopic organism B5 holofilms of the standard of making
It is consistent, as shown in Figure 4.Therefore, by the standard microscopic organism on the direction and holofilm of the output light of holofilm
Correspond.
Leisure light 12 is received by a detector through microscopic organism b20 to be measured, and flashlight 13 is by holofilm b7 and quilt
It is received by a detector after being diffracted on different directions.High relevance based on flashlight 13 with leisure light 12, flashlight 13 exists
By being diffracted on different directions after holofilm b 7, if this direction is different from the reference light side of microscopic organism b20 to be measured
To, then can not possibly with leisure light produce coincidence counting.If measuring resulting result respectively R, S, T in three detectors；R is
Leisure light 12 measures resulting result through microscopic organism b20 to be measured by detector 14, and S is that flashlight 13 passes through holographic bottom
The projection of the microscopic organism B5 of the piece b7 Plays and result obtained by being measured by detector 14 after being diffracted comes from light path a
21 measurement result, T is the projection of the microscopic organism A2 that flashlight 13 passes through holofilm b7 Plays and is diffracted rear quilt
Detector 14 measures resulting result i.e. from the measurement result of light path b 22.Leisure light and flashlight are carried out meeting meter
Number, if coming from light path a 21 with the flashlight of leisure light coincidence counting, the result of coincidence counting is expressed as C (R, S), such as
The flashlight of fruit leisure light coincidence counting comes from light path b 22, then the result of coincidence counting is expressed as C (R, T).Experimental result
Show, if the microscopic organism placed is microscopic organism A, the result of coincidence counting is entirely C (R, T)；If instead
The microscopic organism of placement is microscopic organism B, then the result of coincidence counting is entirely C (R, S).Therefore, we can pass through
The result of coincidence counting effectively tells microscopic organism to be measured.
Additionally, adjustable filter plate 19 is placed between microscopic organism b20 to be measured and bbo crystal 11, it is microcosmic according to this
Organism is adjusted selecting to the ability to bear of light to intensity, polarization of the light 12 that lies fallow etc., and it is right to be so effectively prevented from
The damage of microscopic organism；Pass through holofilm b7 and be diffracted into the letter on different directions in holofilm b7 and each measurement
An adjustable filter plate 19 is respectively placed between the detector 14 of number light, each filter plate can be according to the filter in leisure light light path
The setting of wave plate carries out the regulation such as corresponding intensity, polarization, the purpose for the arrangement is that can with selection signal light 13 with pass through
The corresponding association photon of leisure light 12, so as to suppress noise, improves accuracy.
Above is being illustrated as a example by identifying microscopic organism to be measured from two kinds of different microscopic organisms.At this
In invention, the method same with the first step can also be utilized to project on holofilm multiple microscopic organisms, then with
The same method operation of two steps, and by flashlight by after the holofilm that multiple microscopic organisms are projected, being exposed in gradation
What is used during light holography is multiple with reference to a general intensity detector is respectively placed on light direction, then uses identical method
Coincidence counting.Respectively an adjustable filtering can be placed between the detector in the light path of holofilm and each flashlight
Piece, each filter plate can carry out the regulation such as corresponding intensity, polarization according to the setting of the filter plate in leisure light light path.So
Just microscopic organism to be measured is identified using the same method of the first step and second step from various different microscopic organisms.
Claims (4)
1. a kind of method that microscopic organism is distinguished based on Quantum Correlation imaging technique, it is characterised in that step is as follows：
Step one, one multiple exposure holofilm of acquisition
A kind of microscopic organism of standard is placed in hologram photoplate front, the microscopic organism of standard is irradiated with laser beam,
Light beam after transmission is projected on a holofilm jointly with the laser beam of another Shu Zuowei reference beams；Repeat this mistake
Journey, different microscopic organisms is projected on same holofilm, the reference beam side that different microscopic organisms is used
To difference, i.e., different reference light direction is unique to should microscopic organism；Egative film development, fixing treatment are obtained and exposed by several times
Light holofilm；
Step 2
Entangled photon pairs light path is built, including is irradiated on bbo crystal with a branch of pumping laser and is produced a pair of parametric down conversions
Entangled photon pairs, are referred to as leisure light and flashlight；Microscopic organism to be measured is placed in the light path of leisure light, multiple exposure
Holofilm is put into the light path of flashlight, to the leisure light by microscopic organism to be measured and by multiple exposure holofilm
And be diffracted into after the flashlight on different directions is utilized respectively common intensity detector measurement, and meet measurement result meter
Number can tell microscopic organism to be measured；Microscopic organism to be measured need to be to project to the microcosmic life on multiple exposure holofilm
One kind of object.
2. a kind of method that microscopic organism is distinguished based on Quantum Correlation imaging technique according to claim 1, its feature
It is that the microscopic organism projected on the multiple exposure holofilm is two or more.
3. a kind of method that microscopic organism is distinguished based on Quantum Correlation imaging technique according to claim 1, its feature
It is in the light path of the leisure light, adjustable filter plate to be placed between microscopic organism to be measured and bbo crystal.
4. a kind of method that microscopic organism is distinguished based on Quantum Correlation imaging technique according to claim 1, its feature
It is, the common strength investigation in the light path of the flashlight, in the light path of multiple exposure holofilm and each flashlight
Adjustable filter plate is placed between device.
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| CN201710156195.2A CN106841013B (en) | 2017-03-16 | 2017-03-16 | A method of microscopic organism is distinguished based on Quantum Correlation imaging technique |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107831144A (en) * | 2017-10-20 | 2018-03-23 | 上海理工大学 | Optical filter transmitance detection method based on the imaging of compressed sensing association in time |
| CN108981935A (en) * | 2017-12-28 | 2018-12-11 | 三维通信股份有限公司 | A kind of implementation method tangled based on the two-photon higher dimensional space converted under Spontaneous Parametric |
| CN114384696A (en) * | 2021-12-27 | 2022-04-22 | 南京波长光电科技股份有限公司 | Novel dual-ion ultraviolet fluorescence imaging optical system |
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| CN105068397A (en) * | 2015-09-14 | 2015-11-18 | 中国工程物理研究院激光聚变研究中心 | Quantum cascade laser terahertz source digital holographic imaging system |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107831144A (en) * | 2017-10-20 | 2018-03-23 | 上海理工大学 | Optical filter transmitance detection method based on the imaging of compressed sensing association in time |
| CN107831144B (en) * | 2017-10-20 | 2020-02-21 | 上海理工大学 | Optical filter transmittance detection method based on compressed sensing time correlation imaging |
| CN108981935A (en) * | 2017-12-28 | 2018-12-11 | 三维通信股份有限公司 | A kind of implementation method tangled based on the two-photon higher dimensional space converted under Spontaneous Parametric |
| CN108981935B (en) * | 2017-12-28 | 2020-07-21 | 三维通信股份有限公司 | Method for realizing two-photon high-dimensional space entanglement based on spontaneous parametric down-conversion |
| CN114384696A (en) * | 2021-12-27 | 2022-04-22 | 南京波长光电科技股份有限公司 | Novel dual-ion ultraviolet fluorescence imaging optical system |
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