CN106841013B - A method of microscopic organism is distinguished based on Quantum Correlation imaging technique - Google Patents
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 6
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- 238000005086 pumping Methods 0.000 claims description 6
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
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Abstract
The invention discloses a kind of methods for distinguishing microscopic organism based on Quantum Correlation imaging technique, this method is to project to a series of image of different microscopic organisms on one holofilm by multiple exposure holographic technique, then the hologram of microscopic organism to be measured and preparation is individually positioned in the entangled photon pairs optical path generated by parametric down conversion process, wherein microscopic organism to be measured is placed in the optical path of leisure light, hologram is placed in the optical path of signal light, using common intensity detector to by the leisure light of object under test and passing through hologram and be diffracted into the progress of the signal light on different directions coincidence measurement and can distinguish tested organism.Due to using general intensity detector in the process, and signal light is not contacted with imaging object, and this method can be realized high-resolution, low cost imaging.
Description
Technical field
The present invention relates to it is a kind of based on Quantum Correlation imaging technique distinguish microscopic organism method, belong to quantum optices and
The interleaving techniques field of imaging technique.
Background technique
Micro-imaging is a kind of means observed and identify small items, and classical optics imaging technique is established in electromagnetic wave
On certainty theory model (geometric optics, wave optics) and classical theory of information basis, by the average light for recording radiation field
The image information of (or position phase) distributed acquisition target by force.The imaging detection sensitivity of classical optics imaging technique can not surmount detection
The quantum noise limit of system, Optical Resolution of Imaging System can not surmount its resolution diffraction limit, and its scanning imagery speed
Rate can not surmount the nyquist sampling limit of classical theory of information.
Relative to the image letter for obtaining target in traditional optical imaging technique by the light distribution of record radiation field
The method of breath, quantum imaging are then to obtain the image of object by utilizing, controlling the quantum fluctuation of (or simulation) radiation field.Beauty
Shi Yanhua group, University of Maryland, state experimentally realizes relevance imaging in nineteen ninety-five for the first time, utilizes wavelength 351.1nm, laser
The argon ion laser of spot diameter 2mm pumps II type bbo crystal as pump light source, generates tangling for a pair of of parametric down conversion
Photon pair, referred to as leisure light and signal light, by a polarization beam apparatus, signal light and reference light are divided into two-way, in reference light
Microscopic organism to be measured is placed in optical path, by record two optical paths coincidence counting, can be obtained object clearly as.Although letter
Number light, can at signal light end since signal light and leisure light are highlights correlations contacting with microscopic organism to be measured
The characteristic information of microscopic organism to be measured is presented.Quantum Correlation imaging technique can achieve or break through diffraction limit, pass through symbol
Close measurement can effectively compression noise realize Resolving measurement.But the technology need it is high-precision
CCD camera, so that distinguishing that cost increases.
Summary of the invention
To solve the above-mentioned problems, the present invention provides a kind of side that microscopic organism is distinguished based on Quantum Correlation imaging technique
Method, this method can rapidly and accurately identify microscopic organism under the premise of low cost.
A kind of the step of method distinguishing microscopic organism based on Quantum Correlation imaging technique according to the present invention are as follows:
Step 1: obtaining a multiple exposure holofilm
A kind of microscopic organism of standard is placed in front of hologram photoplate, with the microbiological of laser beam irradiation standard
The laser beam of body, light beam and another Shu Zuowei reference beam after transmission is projected jointly on a holofilm；Repeat this
One process projects to different microscopic organisms on same holofilm, the reference light that different microscopic organisms uses
Shu Fangxiang is different, i.e., different reference light directions uniquely correspond to the microscopic organism；Egative film development, fixing processing are divided
Secondary exposed holographic egative film；
Step 2
Entangled photon pairs optical path is constructed, including is irradiated on bbo crystal with a branch of pumping laser and generates a pair of of lower turn of parameter
The entangled photon pairs changed are referred to as leisure light and signal light；Microscopic organism to be measured is placed in the optical path of leisure light, by several times
Exposed holographic egative film is put into the optical path of signal light, to the leisure light by microscopic organism to be measured and passes through multiple exposure holography
Egative film is simultaneously diffracted into after the signal light on different directions is utilized respectively common intensity detector measurement, and is accorded with to measurement result
Total number can tell microscopic organism to be measured；Microscopic organism to be measured need to be project to it is micro- on multiple exposure holofilm
See one kind of organism.
Further, the microscopic organism projected on the multiple exposure holofilm is two or more.
Further, adjustable filtering is placed in the optical path of the leisure light, between microscopic organism to be measured and bbo crystal
Piece.
Further, in the optical path of the signal light, general in the optical path of multiple exposure holofilm and each signal light
Adjustable filter plate is placed between logical intensity detector.
Compared with prior art, multiple exposure holographic technique and Quantum Correlation imaging technique are innovatively combined and are answered by the present invention
For the discrimination of microscopic organism, due to using general intensity detector in the process, and signal light does not connect with imaging object
Touching, realizes under the premise of low cost, quick, high-resolution and high sensitivity measurement.
Detailed description of the invention
Fig. 1 is the microscopic organism A multiple exposure holography process schematic of standard；
Fig. 2 is the microscopic organism B multiple exposure holography process schematic of standard；
Fig. 3 is the microscopic organism A holofilm read-out principle schematic diagram of standard；
Fig. 4 is the microscopic organism B holofilm read-out principle schematic diagram of standard；
Fig. 5 is Quantum Correlation image-forming principle schematic diagram；
Fig. 6 is the schematic diagram that microscopic organism is distinguished based on multiple exposure holographic imaging and Quantum Correlation imaging technique；
Wherein 1 is laser beam, and 2 be microscopic organism A, and 3 be reference beam a, and 4 be holofilm a, and 5 be microscopic organism
B, 6 be reference beam b, and 7 be holofilm b, and 8 be output light a, and 9 be output light b, and 10 be pumping laser, and 11 be bbo crystal, 12
It is signal light for the light that lies fallow, 13,14 be detector, and 15 be CCD camera, and 16 be coincidence counting device, and 17 be microscopic organism to be measured
A, 18 be the imaging of microscopic organism a to be measured, and 19 be adjustable filter plate, and 20 be microscopic organism b to be measured, and 21 be optical path a, 22
For optical path b.
Specific embodiment
The present invention is described in detail with reference to the accompanying drawing, and content described herein is only used for describing and explaining this hair
It is bright, it is not intended to limit the present invention.
Step 1: obtaining a holofilm for having numerous microscopic organism projections.It is specific as follows:
The microscopic organism A2 for irradiating standard with laser beam 1 first, as shown in Figure 1, the microscopic organism A2 of standard is set
Immediately ahead of hologram photoplate, the microscopic organism A2 of 1 vertical irradiation standard of laser beam projected the microscopic organism of standard
The laser beam of A2 forms the object beam of diffusion formula, and another laser beam is mapped on egative film as reference beam a 3 and object beam is folded
Add generation interference, the position phase and amplitude of each point on object light wave are converted into the intensity being spatially varying, to utilize interference
The all information of object light wave is recorded at interfringe contrast and interval.The object beam and reference beam a 3 of transmission are common
It projects on holofilm, forms the holofilm a 4 of the interference fringe of the microscopic organism A2 for the standard that records.We utilize
Same process projects the microscopic organism B 5 of standard on same holofilm, is not uniquely both using reference light
Beam b 6, the reference light, that is, direction of reference beam a 3 of microscopic organism A2 in direction and standard of reference light is different, such as Fig. 2
It is shown.Diverse microcosmic bioagent reference light direction is different, i.e., different reference light directions uniquely correspond to the microscopic organism.In this way
Just completed the multiple exposure holofilm b for possessing that the microscopic organism A2 of standard and the microscopic organism B5 of standard are projected
7.Multiple and different images can also be recorded on same egative film by multiple exposure, and can be shown respectively without interfering with each other
Out, require the direction of reference beam cannot be identical in the process.This process can be repeated as many times, therefore this method can be with
Numerous microscopic organisms is projected on a holofilm.Egative film is handled using programs such as development, fixings later.This shows
It is intended to and subsequent schematic diagram is illustrated the technology for distinguishing microscopic organism A2 and microscopic organism B5, but
It is not limited thereto, illustrates hereby.
Step 2: building entangled photon pairs optical path, is irradiated on bbo crystal with a branch of pumping laser and is generated under a pair of of parameter
The entangled photon pairs of conversion are referred to as leisure light and signal light.To the leisure light by microscopic organism to be measured and by complete
It ceases egative film and is diffracted into the signal light on different directions and be utilized respectively detector progress coincidence measurement.It is specific as follows:
The schematic illustration of Quantum Correlation imaging is incited somebody to action as shown in figure 5, being irradiated on bbo crystal 11 with a branch of pumping laser 10
Generate the entangled photon pairs of a pair of of parametric down conversion, referred to as leisure light 12 and signal light 13.By microscopic organism a 17 to be measured
It is placed in the optical path of leisure light 12, the leisure light 12 passed through detector 14 is measured, detector 14 is the spy of general intensity
It surveys device, need high-resolution.Spacescan measurement is carried out to signal light 13 with high-resolution CCD camera 15 simultaneously, then
Coincidence counting is carried out using both 16 pairs of coincidence counting device, the imaging 18 of high-resolution microscopic organism a to be measured can be presented.
The present invention is the side based on multiple exposure holographic imaging and Quantum Correlation imaging technique rapid identification microscopic organism
Method.As shown in fig. 6, being irradiated to the entangled photons that will generate a pair of of parametric down conversion on bbo crystal 11 with a branch of pumping laser 10
Right, referred to as lie fallow light 12 and signal light 13.Microscopic organism b 20 to be measured is placed in leisure 12 optical path of light, and to logical
The leisure light 12 crossed is measured with detector 14, and detector 14 is general intensity detector, does not need high-resolution.It is to be measured microcosmic
Organism b 20 is one kind of the microscopic organism projected on holofilm.Early period is placed in the optical path of signal light 13 simultaneously
The multiple exposure holofilm b 7 of preparation, after signal light 13 is diffracted on different directions after through holofilm b 7,
It is respectively to be placed on reference beam a3 and the direction reference beam b6 on two reference light directions used in multiple exposure holography process
One detector 14, detector 14 are general intensity detector.Its principle is as shown in Figure 3 and Figure 4.It is irradiated when with monochromatic light beamlet
Microscopic organism A2 allows the light beam of transmission to project on the multiple exposure holofilm b7 prepared, passes through at this time by diffraction
It the direction of the output light a8 of holofilm b7 can be with reference light, that is, reference light of the microscopic organism A2 holofilm of production standard
The direction beam a3 is consistent, as shown in Figure 3.Similarly, monochromatic light beamlet irradiates microscopic organism B5, passes through multiple exposure holofilm
Reference light, that is, direction reference beam b6 of the microscopic organism B5 holofilm in the direction and production standard of the output light b9 after b7
It is consistent, as shown in Figure 4.Therefore, the standard microscopic organism on the direction and holofilm by the output light of holofilm
It corresponds.
Leisure light 12 passes through microscopic organism b20 to be measured and is received by a detector, and signal light 13 passes through holofilm b7 and quilt
It is received by a detector after being diffracted on different directions.High relevance based on signal light 13 and leisure light 12, signal light 13 exist
By being diffracted on different directions after holofilm b 7, if this direction is different from the reference light side of microscopic organism b20 to be measured
To then it is not possible that generating coincidence counting with leisure light.If measuring obtained result in three detectors is respectively R, S, T；R is
Leisure light 12 passes through microscopic organism b20 to be measured and measures obtained as a result, S is that signal light 13 passes through holographic bottom by detector 14
The projection of the microscopic organism B5 of piece b7 Plays simultaneously measures obtained result by detector 14 after being diffracted and comes from optical path a
21 measurement result, T are signal light 13 by the projection of the microscopic organism A2 of holofilm b7 Plays and are diffracted rear quilt
Detector 14 measures measurement result of the obtained result i.e. from optical path b 22.Leisure light and signal light are carried out meeting meter
Number, if with leisure light coincidence counting signal light from optical path a 21, the result of coincidence counting is expressed as C (R, S), such as
The signal light of fruit leisure light coincidence counting is from optical path b 22, then the result of coincidence counting is expressed as C (R, T).Experimental result
Show that the result of coincidence counting is entirely C (R, T) if the microscopic organism placed is microscopic organism A；If instead
The microscopic organism of placement is microscopic organism B, then the result of coincidence counting is entirely C (R, S).Therefore, we can pass through
The result of coincidence counting effectively tells microscopic organism to be measured.
In addition, adjustable filter plate 19 is placed between microscopic organism b20 to be measured and bbo crystal 11, it is microcosmic according to this
Selection is adjusted to intensity, the polarization etc. of leisure light 12 to the ability to bear of light in organism, can be effectively avoided in this way pair
The damage of microscopic organism；Pass through holofilm b7 in holofilm b7 and each measurement and is diffracted into the letter on different directions
An adjustable filter plate 19 is respectively placed between the detector 14 of number light, each filter plate can be according to the filter in leisure light optical path
The setting of wave plate carries out corresponding intensity, polarization etc. and adjusts, the purpose for the arrangement is that can choose in signal light 13 and pass through
Lie fallow the corresponding association photon of light 12, to inhibit noise, improves accuracy.
It is to be illustrated by for identifying microscopic organism to be measured in two different microscopic organisms above.At this
In invention, method same as the first step can also be utilized to project multiple microscopic organisms on holofilm, then with
The same method operation of two steps, and by signal light after the holofilm projected by multiple microscopic organisms, it is exposed in gradation
It is multiple with reference to a general intensity detector is respectively placed on light direction used in light holography process, then with identical method
Coincidence counting.An adjustable filtering can be respectively placed between the detector in the optical path of holofilm and each signal light
Piece, each filter plate can carry out corresponding intensity, polarization etc. according to the setting of the filter plate in leisure light optical path and adjust.In this way
With regard to identifying microscopic organism to be measured from a variety of different microscopic organisms using first step method same as second step.
Claims (4)
1. a kind of method for distinguishing microscopic organism based on Quantum Correlation imaging technique, it is characterised in that steps are as follows: Step 1:
Obtain a multiple exposure holofilm
A kind of microscopic organism of standard is placed in front of hologram photoplate, the microscopic organism of standard is irradiated with laser beam,
The laser beam of light beam and another Shu Zuowei reference beam after transmission is projected jointly on a holofilm；Repeat this mistake
Journey projects to different microscopic organisms on same holofilm, the reference beam side that different microscopic organisms uses
To difference, i.e., different reference light directions uniquely corresponds to the microscopic organism；Egative film development, fixing processing are obtained and exposed by several times
Light holofilm；
Step 2
Entangled photon pairs optical path is constructed, generates a pair of of parametric down conversion on bbo crystal including being irradiated to a branch of pumping laser
Entangled photon pairs are referred to as leisure light and signal light；Microscopic organism to be measured is placed in the optical path of leisure light, multiple exposure
Holofilm is put into the optical path of signal light, to the leisure light by microscopic organism to be measured and passes through multiple exposure holofilm
And it is diffracted into after the signal light on different directions is utilized respectively common intensity detector measurement, and meter is met to measurement result
Number can tell microscopic organism to be measured；Microscopic organism to be measured need to be the microcosmic life projected on multiple exposure holofilm
One kind of object.
2. a kind of method for distinguishing microscopic organism based on Quantum Correlation imaging technique according to claim 1, feature
It is, the microscopic organism projected on the multiple exposure holofilm is two or more.
3. a kind of method for distinguishing microscopic organism based on Quantum Correlation imaging technique according to claim 1, feature
It is, places adjustable filter plate in the optical path of the leisure light, between microscopic organism to be measured and bbo crystal.
4. a kind of method for distinguishing microscopic organism based on Quantum Correlation imaging technique according to claim 1, feature
It is, the common strength investigation in the optical path of the signal light, in the optical path of multiple exposure holofilm and each signal light
Adjustable filter plate is placed between device.
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| CN107831144B (en) * | 2017-10-20 | 2020-02-21 | 上海理工大学 | Optical filter transmittance detection method based on compressed sensing time correlation imaging |
| CN108981935B (en) * | 2017-12-28 | 2020-07-21 | 三维通信股份有限公司 | Method for realizing two-photon high-dimensional space entanglement based on spontaneous parametric down-conversion |
| CN114384696B (en) * | 2021-12-27 | 2023-05-23 | 南京波长光电科技股份有限公司 | Novel double-ion ultraviolet fluorescence imaging optical system |
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| CN105068397A (en) * | 2015-09-14 | 2015-11-18 | 中国工程物理研究院激光聚变研究中心 | Quantum cascade laser terahertz source digital holographic imaging system |
| CN204925626U (en) * | 2015-09-14 | 2015-12-30 | 中国工程物理研究院激光聚变研究中心 | Quantum cascades laser instrument terahertz holographic imaging system of source digit now |
| CN105676613A (en) * | 2016-03-29 | 2016-06-15 | 山东大学 | Digital holography ghost imaging system by adopting single-pixel or bucket detector and working method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105068397A (en) * | 2015-09-14 | 2015-11-18 | 中国工程物理研究院激光聚变研究中心 | Quantum cascade laser terahertz source digital holographic imaging system |
| CN204925626U (en) * | 2015-09-14 | 2015-12-30 | 中国工程物理研究院激光聚变研究中心 | Quantum cascades laser instrument terahertz holographic imaging system of source digit now |
| CN105676613A (en) * | 2016-03-29 | 2016-06-15 | 山东大学 | Digital holography ghost imaging system by adopting single-pixel or bucket detector and working method thereof |
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| Holographic optical coherence imaging of rat osteogenic sarcoma tumor spheroids;Yu, P et al.;《APPLIED OPTICS》;20040901;第43卷(第25期);4862-4873 * |
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