CN106832043A - A kind of preparation method of oat beta glucan extract solution - Google Patents

Abstract

The present invention discloses a kind of preparation method of oat beta glucan extract solution, comprises the following steps：（1）Cleaning, filtering；（2）Enzymolysis, filtering；（3）Freeze thawing removal of impurities, filtering；（4）Wine sinks, filtering；（5）Supercritical CO₂Removal of impurities；（6）Redissolve.Present invention process is simple, low cost, is easy to application, product stabilization, non agricultural chemical residuum.

Description

A kind of preparation method of avenabeta glucosan extract solution

Technical field

The invention belongs to cosmetics plant material technical field, and in particular to a kind of preparation of avenabeta glucosan extract solution Method.

Background technology

Oat beta-glucan is extracted by oat bran, and added value of product increases after extraction.Oat beta-glucan be with β -1, The sticky poly glucan that 3-, β-Isosorbide-5-Nitrae-glycosidic bond are coupled together, it mainly concentrates the aleurone with oat bran.Gather oat Portugal Sugar has various functions, with reduction blood fat, control blood sugar, strengthen immunity, and can prevent constipation, improves intestinal microenvironment Deng, be widely used in medicine and field of health care food.

Patent CN201510115883.5 can preferably prepare the avenabeta glucosan of different molecular weight, but technique is too Complexity, cost is too high to be unfavorable for application；Patent CN201410633854.3, CN200910199481.2, CN201210064262.5 etc. does not remove the avenabeta glucosan of macromolecular, used as avenabeta glucosan liquid used for cosmetic Body, the presence of macromolecular can make product unstable, produced in shelf life and separated out, CN200910199481.2, The membrane filtration technique that CN201210064262.5 is used can substantial amounts of loss active component, and because preceding working procedure cannot eliminate fat The macromolecular substances such as fat, starch, membrane flux can decline quickly, and practical application is very inconvenient, and current patent is not weighed in addition Depending on conventional filtration problem and Pesticide Residue.

The content of the invention

To solve the problems, such as prior art, the present invention provides a kind of preparation method of avenabeta glucosan extract solution, Present invention process is simple, low cost, is easy to application, product stabilization, non agricultural chemical residuum.

To achieve the above object, the technical solution adopted by the present invention is：

A kind of preparation method of avenabeta glucosan extract solution, comprises the following steps：

（1）Cleaning, filtering：Oat bran is cleaned 3 ~ 4 times for 8 ~ 10 times with running water, filter residue is collected in filtering；

（2）Enzymolysis, filtering：By step（1）In filter residue press solid-liquid ratio 1:4~1:6 ratio adds water, is subsequently adding α-middle temperature Amylase enzymolysis, hydrolysis temperature is：50 ~ 55 DEG C, the consumption of α-middle temperature amylase is 10 ~ 20U/g oat brans, enzymolysis time For：2h, enzymolysis pH are：4 ~ 6, filtrate is collected in filtering；

（3）Freeze thawing removal of impurities, filtering：By step（2）In filtrate at a temperature of -20 ~ -5 DEG C, freeze 24 ~ 48h, filtering, collect Filtrate；

（4）Wine sinks, filtering：By step（3）Filtrate concentration be that 95% ethanol wine sinks, solution reaches 30 ~ 40 ° after alcohol precipitation, cold Hide overnight, filter residue is collected in filtering；

（5）Supercritical CO₂Removal of impurities：By step（4）Filter residue mix 5 ~ 15% activated carbon, then use supercritical CO₂Removal of impurities, removal of impurities Pressure is 25MPA, and removal of impurities temperature is 35 DEG C, and except miscellaneous time is 2h, removal of impurities flow is 2000L/H；

（6）Redissolve：By step（5）Treated filter residue adds 95 times of pure water, and 1.5 ~ 2h, mistake are stirred under 95 DEG C of temperature conditionss Filter, have collected filtrate, and it is 1 that benefit adds water to wheat bran and the final liquid proportional that extracts:50, obtain final product avenabeta glucosan extract solution.

Further, the step（5）Activated carbon be 100 ~ 200 mesh.

Beneficial effects of the present invention：

（1）Process is simple, low cost：Enzyme dosage is few, and supercritical extract treating capacity is few, does not have the steps such as ultrafiltration, freeze-drying, behaviour Facilitate；

（2）It is applied strong, focus on starch, macromolecular polysaccharide and albumen, the removal of fat, filtering is easier；

（3）Product stabilization：By CO₂Extraction removes fat, and alcohol dissolubility impurity is removed by the way that wine is heavy；Macromolecular is removed by freeze thawing Glucan and albumen；

（4）Safety：Remove residues of pesticides；

（5）Whole technique glucan loss is few, and recovery rate is high, step is more and the operation such as ultrafiltration all can substantial amounts of loss Portugal gather Sugar, makes cost increase.

Specific embodiment

For technological means, creation characteristic, reached purpose and effect for making present invention realization are easy to understand, with reference to Specific embodiment, is expanded on further the present invention, but the present invention includes but is not limited to the embodiment.

Embodiment 1：Oat bran adds 8 times of running water to clean 4 times, and filter residue adds α-middle temperature amylase enzymolysis to extract, 55 DEG C of temperature, The dry oat brans of consumption 10U/g, digest 2h, and PH is 4, and 4 times of amounts of purified water, extraction is filtered after terminating, -20 DEG C of freezing 24h of filtrate, Take advantage of cold filtering after dissolving, filtrate concentration is that 95% ethanol wine sinks, and solution reaches 30 °, refrigerated overnight, filtering, filter residue after alcohol precipitation Mix 5% 200 mesh activated carbon supercritical COs₂Removal of impurities, operating pressure 25MPA, 35 DEG C of temperature, time 2h, flow 2000L/H, extraction Excess adds 95 times of purified waters, 95 DEG C of stirring 1.5h, and moisturizing to wheat bran and the final liquid proportional that extracts is 1 after filtering:50, obtain final product oat Beta glucan extract solution.

Embodiment 2：Oat bran adds 10 times of running water to clean 3 times, and filter residue adds α-middle temperature amylase enzymolysis to extract, temperature 50 DEG C, the dry oat brans of consumption 20U/g digest 2h, and PH is 6, and 6 times of amounts of purified water, extraction is filtered after terminating, -5 DEG C of freezings of filtrate 48h, takes advantage of cold filtering after dissolving, filtrate concentration is that 95% ethanol wine sinks, and solution reaches 40 ° after alcohol precipitation, refrigerated overnight, filtering, Filter residue mixes 15% 200 mesh activated carbon supercritical COs₂Removal of impurities, operating pressure 20MPA, 40 DEG C of temperature, time 1h, flow 1500L/H, extract remainder adds 85 times of purified waters, 95 DEG C of stirring 2h, and moisturizing to wheat bran and the final liquid proportional that extracts is 1 after filtering:50, Obtain final product avenabeta glucosan extract solution.

Embodiment 3：Oat bran adds 9 times of running water to clean 4 times, and filter residue adds middle temperature amylase enzymolysis to extract, and 53 DEG C of temperature is used The dry oat brans of 15U/g are measured, 2h is digested, PH is 5,5 times of amounts of purified water, extraction is filtered after terminating, and -10 DEG C of filtrate freezes 36h, molten Take advantage of cold filtering after solution, filtrate concentration is that 95% ethanol wine sinks, and solution reaches 35 ° after alcohol precipitation, and refrigerated overnight, filtering, filter residue is mixed 10% 100 mesh activated carbon supercritical COs₂Removal of impurities, operating pressure 25MPA, 35 DEG C of temperature, time 2h, flow 2000L/H, extraction Excess adds 95 times of purified waters, 95 DEG C of stirring 2h, and moisturizing to wheat bran and the final liquid proportional that extracts is 1 after filtering:50, obtain final product oat β- Glucan extract solution.

Comparative example 1：Oat bran crosses 60 mesh sieves and takes non-screened part, plus 25 times of water, and PH is 7.5,45 DEG C of stirring 2h, 10000 leave the heart after take filtrate, be cooled to 20 DEG C, PH is adjusted to 5.0, stands 0.5h after stirring, 5000 leave the heart after clear liquid add Low temperature alpha amylase enzymolysis and extraction, 35 DEG C of temperature, the dry oat bran of PH5.5, consumption 200U/g digests 1.5h, extracts mistake after terminating Filter, filtrate uses 80000Da ultrafiltration membrance filters, trapped fluid freeze-drying, 95 DEG C of stirring 1.5h of purified water, moisturizing to wheat bran and final It is 1 to extract liquid proportional:50, obtain final product avenabeta glucosan extract solution.

Comparative example 2：Oat bran is crushed to 60 ~ 100 mesh, plus 9 times of deionized waters, adjusts PH=6, increases temperature alpha amylase enzymolysis Extract, 85 DEG C of temperature, PH is 6.0, the dry oat brans of consumption 540U/g, digest 10min, be cooled to 60 DEG C, PH is adjusted to 4.5, Plus glucoamylase（390ASPU/g、260GAU/g）The dry oat brans of 0.8g/100g, digest 2h, are filtered after terminating, and filtrate is used 80000Da ultrafiltration membrance filters, trapped fluid spray drying, 95 DEG C of stirring 1.5h of purified water, moisturizing to wheat bran and final extract solution ratio Example is 1:50, obtain final product avenabeta glucosan extract solution.

Comparative example is using disclosed prior art.

Implementation result contrast such as following table：Study on the stability condition：- 18 ~ 48 DEG C of alternatings, 48 DEG C, 4 DEG C, UV

	Total filtration time/hour	Stability/day	Beta-dextran content/%
				Embodiment 1	8	35	1.02
Embodiment 2	7.5	42	0.98
				Embodiment 3	7.5	37	1.05
Comparative example 1	9	29	0.63
				Comparative example 2	14	7	0.87

The present invention passes through step（1）Most of starch is washed away, step enzyme dosage 2., rate-determining steps is reduced（2）Temperature make it is few The starch of amount is not gelatinized, and is easy to the filtering of each step；Alcohol dissolubility impurity is removed by the way that wine is heavy；Degreasing is removed by SCF-CO 2 Fat and residues of pesticides, make product safety, stabilization, and bigger than directly carrying out extraction cost to wheat bran in this stepping row CO2 extractions Amplitude reduction, because of enrichment, impurity-eliminating effect is also relatively much higher；Macromolecular glucan and albumen are removed by freeze thawing, makes product Product are more stablized.

General principle of the invention and principal character and advantages of the present invention has been shown and described above.The technology of the industry Personnel it should be appreciated that the present invention is not limited to the above embodiments, simply explanation described in above-described embodiment and specification this The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appending claims and its Equivalent thereof.

Claims (2)

1. a kind of preparation method of avenabeta glucosan extract solution, it is characterised in that comprise the following steps：

（1）Cleaning, filtering：Oat bran is cleaned 3 ~ 4 times for 8 ~ 10 times with running water, filter residue is collected in filtering；

（2）Enzymolysis, filtering：By step（1）In filter residue press solid-liquid ratio 1:4~1:6 ratio adds water, is subsequently adding α-middle temperature Amylase enzymolysis, hydrolysis temperature is：50 ~ 55 DEG C, the consumption of α-middle temperature amylase is 10 ~ 20U/g oat brans, enzymolysis time For：2h, enzymolysis pH are：4 ~ 6, filtrate is collected in filtering；

（3）Freeze thawing removal of impurities, filtering：By step（2）In filtrate at a temperature of -20 ~ -5 DEG C, freeze 24 ~ 48h, filtering, collect Filtrate；

（4）Wine sinks, filtering：By step（3）Filtrate concentration be that 95% ethanol wine sinks, solution reaches 30 ~ 40 ° after alcohol precipitation, cold Hide overnight, filter residue is collected in filtering；

（5）Supercritical CO₂Removal of impurities：By step（4）Filter residue mix 5 ~ 15% activated carbon, then use supercritical CO₂Removal of impurities, removal of impurities pressure Power is 25MPA, and removal of impurities temperature is 35 DEG C, and except miscellaneous time is 2h, removal of impurities flow is 2000L/H；

（6）Redissolve：By step（5）Treated filter residue adds 95 times of pure water, and 1.5 ~ 2h, mistake are stirred under 95 DEG C of temperature conditionss Filter, have collected filtrate, and it is 1 that benefit adds water to wheat bran and the final liquid proportional that extracts:50, obtain final product avenabeta glucosan extract solution.

2. a kind of preparation method of avenabeta glucosan extract solution according to claim 1, it is characterised in that the step （5）Activated carbon be 100 ~ 200 mesh.