CN106831091A - A kind of geobiont repair - Google Patents
A kind of geobiont repair Download PDFInfo
- Publication number
- CN106831091A CN106831091A CN201611173685.5A CN201611173685A CN106831091A CN 106831091 A CN106831091 A CN 106831091A CN 201611173685 A CN201611173685 A CN 201611173685A CN 106831091 A CN106831091 A CN 106831091A
- Authority
- CN
- China
- Prior art keywords
- amino acid
- weight portion
- weight
- repair agent
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of geobiont repair agent, it is made up of wax-shaped bacillus bacterium powder, pythium oligandrum bacterium powder, Trichoderma viride bacterium powder, Sphingobacterium multivorum fermentate, edible fungi residue, amino acid chelated calcium, zinc-amino acid chelate and DA 6, it can degrade " oil-agriculture " pollution and residues of pesticides, it is applied to that in farmland diseases prevention of improving the soil can be reached, drops residual growth-promoting, and then improve soil micro-ecosystem activity and self-repairing capability, increase crop yield, improving quality, safeguard the purpose of Agro-ecology health.
Description
【Technical field】
The invention belongs to field of agricultural microbial technology.More particularly it relates to a kind of geobiont repair agent.
【Background technology】
Soil is important carrier of the material conversion with energy exchange and place in agricultural production, is also the core of the ecosystem
Part, in various environmental key-elements, soil is again the final receptor of pollutant.As petroleum product and chemical pesticide are on farmland
Extensive use, the petroleum pollution being retained in soil and pesticide residue are because it is difficult to eradicate and turns into urgently to be resolved hurrily
Problem.
The deterioration of the ecological environment that oil-agriculture is caused, the salinization of soil is hardened, pest and disease damage spreads unchecked, white pollution serious, agriculture
A series of threats of outstanding problems to agricultural sustainable development such as product quality decline are increasing.With development in science and technology, China
The propulsion of agricultural modernization, arable soil Quality Down is obvious all the more, and scope is more and more wider, and its degree is increasingly severe, agricultural
Faced In Sustainable Development is seriously threatened.One side soil nutrient is unbalance, structure is destroyed, soil hardening.On the other hand because soil is dirty
Dye has the features such as disguise, hysteresis quality, not only reduces soil quality, and agricultural is suffered a loss, remains of pesticide
Into water body and food chain, food security hidden danger is caused, threaten the healthy of people.The Shandong that in May, 2013 produces
The event of the malicious ginger of the red production of Weifang peasant abuse legendary god of farming is exactly typical case.
Being faced with China's agricultural future development should keep high yield, and the dual-pressure of resource pollution must be reduced again.Compel
Be essential want feasible technical scheme degraded agricultural production in chemical fertilizer and pollution by pesticides residual, improve soil service efficiency, ensure soil
Earth health, builds the material base of support agricultural sustainable development.
Therefore, people carry out substantial amounts of research to soil issues, obtain some important achievements.Such as CN105950159A is public
A kind of functionalization soil conditioner and preparation method are opened, hardened soil, alkaline land soil, heavy metal polluted soil is the method achieve
The conditioning of earth.CN106047352A discloses a kind of soil conditioner containing water-loss reducer and the side of original soil greening is carried out to slight alkaline land
Method.CN104817385A discloses a kind of garden waste biomass carbon base soil conditioner and preparation method, and it has nutrition unit
Element release coordination is lasting, fertilizer nutrient utilization is high, and effectively improvement and fertilizing soil, increase organic matter, improve soil water-retaining
Fertilizer-preserving ability, improves crop quality, improves crop yield.
The technical bottleneck of restriction soil improvement and recovery technique product industry mainly has two aspects, and one is Agro-ecology ring
Border be destroyed, part Soil Microorganism is unbalance, self-repairing capability decline, soil improvement and repair beneficial microorganism application
Technology cannot much meet the adverse effect caused because soil micro-ecosystem is destroyed, and most soil improvements are micro- with what is repaired
Biologic applications technology forms effective product few still in conceptual phase；Two is the fertilising because fertilizer practice is not matched with fertilizer variety
Technological means falls behind, and causes chemical fertilizer serious disproportion, chemical fertilizer input benefit to be decreased obviously.In fertilising, there is great amount unit
The phenomenon of trace element fertilizer, causes soil hardening during plain fertilizer is light, is unfavorable for that crop absorbs, and then formation soil environment is asked
Topic.
The present invention is directed to these problems, on the basis of prior art is summarized, by lot of experiments research and analysis, finally
Complete the present invention.
【The content of the invention】
[technical problem to be solved]
It is an object of the invention to provide a kind of geobiont repair agent.
[technical scheme]
The present invention is achieved through the following technical solutions.
The present invention relates to a kind of geobiont repair agent.
The geobiont repair agent is by 1-2 weight portion wax-shaped bacillus bacterium powder, 6-12 weight portion pythium oligandrums bacterium powder, 6-12 weights
Amount part Trichoderma viride bacterium powder, 4-8 weight portion Sphingobacterium multivorums fermentate, 50-60 weight portions edible fungi residue, 7-15 weight
Part amino acid chelated calcium, 4-10 weight portions zinc-amino acid chelate and 0.10-0.50 weight portions DA-6 compositions.
A preferred embodiment of the invention, described bacillus cereus bacterium powder viable count >=200.0 hundred million/g,
The fermentation of pythium oligandrum bacterium powder viable count >=10.0 hundred million/g, Trichoderma viride bacterium powder viable count >=10.0 hundred million/g and Sphingobacterium multivorum
Thing >=5.0 hundred million/g.
Another preferred embodiment of the invention, Sphingobacterium multivorum fermentate is to prepare as steps described below
Obtain：
A, activation culture
Prepare beef extract-peptone fluid nutrient medium：5g beef extracts, 10g peptones, 10g glucose, 5g NaCl are added to
In 1000ml distilled water, boil and dissolve, then sterilize 30min at 121 DEG C of temperature, obtains beef extract-peptone Liquid Culture
Base；
Under sterile conditions, 0.1g Sphingobacterium multivorums are inoculated into the above-mentioned beef extract-peptone liquid trainings of 200mL
Support in base, 48h is cultivated under conditions of 30 DEG C of temperature with rotating speed 180r/min, obtain Sphingobacterium multivorum activation culture thing；
B, strain purification
Under sterile conditions, in the Sphingobacterium multivorum activation culture thing for being obtained from step A using aseptic inoculation ring
The a small amount of culture of picking is added in beef-protein medium flat board, line, permanent at 30 DEG C of temperature in the constant incubator
Temperature culture 48h, obtains pure Sphingobacterium multivorum culture；
C, seed liquor culture
Prepare seed liquid culture medium：6g MgSO4.·7H2O, 6g NaCl, 20g bean cake powder are well mixed with 10g corn flour,
1000mL dissolvings are added water to, the pH of its solution is adjusted to 7.1, then sterilize 30min at 121 DEG C of temperature, obtains seed liquor
Culture medium；
From the single Sphingobacterium multivorum colony inoculation of picking on the beef-protein medium flat board that step B is obtained to
In above-mentioned seed liquid culture medium, the concussion and cultivate 36h under conditions of 30 DEG C of temperature with rotating speed 220r/min obtains many food sphingols
Bacillus inoculum；
D, fermented and cultured
Wheat bran, corn flour, bean cake powder compare 1-4.5 according to weight：0.5-2：0.5-1.5 be well mixed, add water regulation to
Water content is 40-50%, sterilizing by weight, obtains solid fermentation culture medium；
Then, it is the 8-10% in terms of solid fermentation culture medium weight according to inoculum concentration, many food sphingols that step C is obtained
Bacillus inoculum is inoculated into the solid fermentation culture medium, and solid fermentation training is carried out under conditions of 28-35 DEG C of temperature
Support 3-4d, the solid fermentation cultured products for obtaining then carry out machinery turn, low temperature drying and crushing, obtain described in many food sheaths
Ammonia alcohol bacillus fermentation thing.
Another preferred embodiment of the invention, in step D, the solid fermentation culture is in temperature 30-
Low temperature drying is carried out under conditions of 38 DEG C.
Another preferred embodiment of the invention, described edible fungi residue is mushroom bacteria residue or flat mushroom bacteria residue.
Another preferred embodiment of the invention, described amino acid chelated calcium is according to following preparation process systems
For what is obtained：
80 DEG C of temperature is heated water in a kettle., according to compound amino acid and calcium salt part mol ratio 1：2 toward in water
Compound amino acid and calcium salt are added, is completely dissolved, then adjust to 8.0 the pH of its solution, chelated under temperature 70 C
Reaction 60min, then concentrated, dry, crushing, obtain amino acid chelated calcium.
Another preferred embodiment of the invention, described zinc-amino acid chelate is according to following preparation process systems
For what is obtained：
40 DEG C of temperature is heated water in a kettle., according to compound amino acid and zinc salt part mol ratio 2：1 toward in water
Compound amino acid and zinc salt are added, is completely dissolved, then adjust to 7.0 the pH of its solution, chelated at 35 DEG C of temperature
Reaction 30min, then concentrated, dry, crushing, obtain zinc-amino acid chelate.
Another preferred embodiment of the invention, the geobiont repair agent is by the waxy bar of 1.2-1.8 weight portions
Bacterium bacterium powder, 8-10 weight portion pythium oligandrums bacterium powder, 8-10 weight portion Trichoderma virides bacterium powder, 5-7 weight portion Sphingobacterium multivorums
Fermentate, 52-58 weight portions edible fungi residue, 9-13 weight portions amino acid chelated calcium, 5-9 weight portions zinc-amino acid chelate with
0.20-0.40 weight portions DA-6 is constituted.
Another preferred embodiment of the invention, the geobiont repair agent is by 1.5 weight portion wax-shaped bacillus bacterium
Powder, 9 weight portion pythium oligandrum bacterium powders, 9 weight portion Trichoderma viride bacterium powders, 6 weight portion Sphingobacterium multivorum fermentates, 54-56
Weight portion edible fungi residue, 10-12 weight portions amino acid chelated calcium, 6-8 weight portions zinc-amino acid chelate and 0.28-0.32 weight
Part DA-6 compositions.
Another preferred embodiment of the invention, total living bacteria count >=0.5 hundred million of geobiont repair agent of the present invention/
G, cellulase activity >=90.0U/g, protease activity >=30.0U/g, calcium content by weight >=0.50%, Zn content by weight
>=0.10%, fineness (≤3mm) >=70.0%, pH 5.5~8.5, water content is by weight≤35.0%.
The present invention is described next in more detail.
The present invention relates to a kind of geobiont repair agent.
The geobiont repair agent is by 1-2 weight portion wax-shaped bacillus bacterium powder, 6-12 weight portion pythium oligandrums bacterium powder, 6-12 weights
Amount part Trichoderma viride bacterium powder, 4-8 weight portion Sphingobacterium multivorums fermentate, 50-60 weight portions edible fungi residue, 7-15 weight
Part amino acid chelated calcium, 4-10 weight portions zinc-amino acid chelate and 0.10-0.50 weight portions DA-6 compositions.
Its Classification And Nomenclature of Bacillus cereus is Bacillus cereus Y1 (Bacillus＆nbsp；cereus ；Y1),
Antibacterial material can be produced, suppresses harmful microorganism breeding, the harmful components in soil of degrading, the bacterial strain can be in the first cyanogen of high concentration
Under chrysanthemum ester environment grow, and can efficient degradation, can be used for by Fenpropathrin pollution water body, the biological cleaning of soil, improve life
State environment.
Main Function of the Bacillus cereus bacterium powder in geobiont repair agent of the present invention is that suppression harmful microorganism is numerous
Grow, repair the water body and soil by Fenpropathrin class pollution by pesticides, improve plant growth environment.
The Bacillus cereus bacterium powder that the present invention is used is the product sold in the market, such as biological by the green section in Jiangsu
Technology Co., Ltd., Cangzhou Wang Fa Bioisystech Co., Ltd, Henan Yangshao Biochemical Engineering Co., Ltd., biology section of Wuhan Toyota
The Bacillus cereus bacterium powder of skill Co., Ltd sale.Detected using the detection method that will be discussed in more detail below and determined, this hair
The bright Bacillus cereus bacterium powder viable count >=20,000,000,000/g for using.
Pythium oligandrum is fungi natural enemy, is a kind of very strong parasitical fungi aggressive present in nature, can be many
Kind of crop root part is colonized, and will not only produce pathogenic effects to crop, and can also suppress or kill other pathomycetes and
Soil-borne pathogen, induction plant produces defense reaction, reduces pathogen invasion.Pythium oligandrum bacterium powder have promote seed sprout,
The beneficial effect of healthy growth suitable environment, promotion root growth is provided for plant, it is also conducive to the ecological balance, uses
It is convenient, significantly improve crop yield, all work in the whole growth cycle of crop, the characteristics of shelf life is long, being thus advantageous to agriculture
The sustainable development of industry, is the important channel for realizing organic agriculture.
Main Function of the pythium oligandrum bacterium powder in geobiont repair agent of the present invention is to produce substantial amounts of enzyme secretion
There is inhibitory action to various disease fungus mycelial growths of plant, reduce the disease incidence of disease, promote plant strain growth.
The pythium oligandrum bacterium powder that the present invention is used is the product sold in the market, for example In Henan Agriculture microorganism work
The pythium oligandrum bacterium powder of journey Technical Research Center production, is detected using the detection method that will be discussed in more detail below and determined, this hair
The bright pythium oligandrum bacterium powder viable count >=1,000,000,000/g for using.
Trichoderma viride is a kind of Hyperparasite, can secrete Multiple Classes of Antibiotics class metabolite, such as viridin, antibacterial
Peptide, gliotoxin etc.；It is also possible to produce various enzyme systems with bioactivity, is one of cellulase-producing activity highest bacterial strain,
Produced cellulase has degradation to plant fiber, and effect is very good；Trichoderma viride is again a kind of resourceful short of money
It is antimicrobial, there is important effect in plant pathology biological control.Existing research work shows, male with Trichoderma viride and widow
Rotten mould Dressing can have good preventive effect to soil-borne disease.
Main Function of the Trichoderma viride bacterium powder in geobiont repair agent of the present invention be accelerate soil in crop material and
The decomposition of feces of livestock and poultry, the native transmissibility fungal disease of preventing and treating.
The Trichoderma viride bacterium powder that the present invention is used is the product sold in the market, such as by Yiyuan member of Imperial Academy's agricultural development
Co., Ltd, Guangzhou Lv Hui bio tech ltd, Shandong Changtai bio tech ltd, Yiyuan Kang Yuan biotechnologies have
The Trichoderma viride bacterium powder of Company is limited, is detected using the detection method that will be discussed in more detail below and determined, what the present invention was used
Trichoderma viride bacterium powder viable count >=1,000,000,000/g.
It is of course also possible to according to prior art literature, be carried out step by step using conventional culture methods using conventional culture medium
Culture, obtains described Bacillus cereus bacterium powder, pythium oligandrum bacterium powder and Trichoderma viride bacterium powder.
Sphingobacterium multivorum belongs to sphingolipid Bacteriaceae, and the bacterium has the ability of degrading crude oil, and can also produce lipopeptid
Class surfactant, with dissolving phosphor and dissolving potassium function, middle trace element that can be in activating soil is improved or extension fertilizer efficiency, is reduced
Fertilizer amount, improves crop anti-adversity, prevention or mitigates plant disease.
Main Function of the Sphingobacterium multivorum fermentate in geobiont repair agent of the present invention is stone in degraded soil
Oil contaminants, improve fertilizer efficiency, and improve crop anti-adversity.
The Sphingobacterium multivorum fermentate that the present invention is used is to prepare as steps described below：
A, activation culture
Prepare beef extract-peptone fluid nutrient medium：5g beef extracts, 10g peptones, 10g glucose, 5g NaCl are added to
In 1000ml distilled water, boil and dissolve, then sterilize 30min at 121 DEG C of temperature, obtains beef extract-peptone Liquid Culture
Base；
Under sterile conditions, by 0.1g by company of bio tech ltd of upper Haifeng county longevity or Shanghai water shield examination biotechnology
The Sphingobacterium multivorum that company of Co., Ltd provides is inoculated into the above-mentioned beef extract-peptone fluid nutrient mediums of 200mL, in temperature
48h is cultivated under conditions of 30 DEG C and rotating speed 180r/min of degree, Sphingobacterium multivorum activation culture thing is obtained.
The sterilizing installation that the present invention is used is product that is usually used in the art, selling in the market, under
Therefore the sterilizing installation that face uses also in this way, repeat no more.
B, strain purification
Under sterile conditions, in the Sphingobacterium multivorum activation culture thing for being obtained from step A using aseptic inoculation ring
The a small amount of culture of picking is added in beef-protein medium flat board, line, permanent at 30 DEG C of temperature in the constant incubator
Temperature culture 48h, obtains pure Sphingobacterium multivorum culture.
Described beef extract-peptone plating medium is culture medium known to those skilled in the art, and it is by 5g
Beef extract, 10g peptones, 5g sodium chloride, 18-20g nutrient agars and 1000ml water are formulated.
The constant incubator that the present invention is used is the product sold in the market, and it is limited for example to win fast industry by Shanghai
The product that company medical instrument factory is sold with trade name biochemical cultivation case.
C, seed liquor culture
Prepare seed liquid culture medium：6g MgSO4.·7H2O, 6g NaCl, 20g bean cake powder are well mixed with 10g corn flour,
1000mL dissolvings are added water to, the pH of its solution is adjusted to 7.1, then sterilize 30min at 121 DEG C of temperature, obtains seed liquor
Culture medium；
From the single Sphingobacterium multivorum colony inoculation of picking on the beef-protein medium flat board that step B is obtained to
In above-mentioned seed liquid culture medium, the concussion and cultivate 36h under conditions of 30 DEG C of temperature with rotating speed 220r/min obtains many food sphingols
Bacillus inoculum；
In the present invention, the pH of seed liquid culture medium, described inorganic base are adjusted using inorganic base or inorganic acid aqueous solution
E.g. NaOH, potassium hydroxide or sodium carbonate, its concentration are typically 1-2N；Described inorganic acid is, for example, phosphoric acid, sulphur
Acid or nitric acid, its concentration are typically 1-2N；
D, fermented and cultured
Wheat bran, corn flour, bean cake powder compare 1-4.5 according to weight：0.5-2：0.5-1.5 be well mixed, add water regulation to
Water content is 40-50%, sterilizing by weight, obtains solid fermentation culture medium；
Then, it is the 8-10% in terms of solid fermentation culture medium weight according to inoculum concentration, many food sphingols that step C is obtained
Bacillus inoculum is inoculated into the solid fermentation culture medium, and solid fermentation training is carried out under conditions of 28-35 DEG C of temperature
Support 3-4d, the solid fermentation cultured products for obtaining then carry out machinery turn, low temperature drying and crushing, obtain described in many food sheaths
Ammonia alcohol bacillus fermentation thing.
The bean cake powder that the present invention is used is bean cake powder or bean or pea bean powder, from China Oil and Food Import and Export Corporation the Huanghai Sea cereal and oil industry (Henan) limited public affairs
Take charge of the bean cake powder that the soybean oil of production is obtained.
Wheat bran, corn flour, bean cake powder are all the products sold in the market, and its granularity is typically 60~300 mesh.
Wheat bran, corn flour mix with bean cake powder used equipment be usually used in the art, in current city
The mixing apparatus sold on field.
In this step, solid fermentation cultured products are preferably carries out low temperature drying under the conditions of 30-38 DEG C of temperature
's.
Detected using the detection method that will be specifically described in detail below and determined, described Sphingobacterium multivorum fermentate
Viable count >=500,000,000/g.
The edible fungi residue used in geobiont repair agent of the invention, i.e. waste material of edible mushroom, also referred to as mushroom bran, be
The plant residue after bacterium mycelia utilizes is eaten after edible fungus culturing, it is extremely difficult to degrade.China at least produces 3,500,000 tons of bacterium every year
Slag, rural area throughout the country, edible fungi residue is typically arbitrarily to be abandoned or burnt, and resulting environmental problem is not allowed to neglect
Depending on.In fact, edible fungi residue is nutritious, its nutrition is equivalent to the nutrition contained by usual wheat bran, dregs of beans.
Geobiont repair agent of the invention, the edible fungi residue Main Function for being used is to improve soil fertility, there is provided
Plant nutrient, improves the content of fruits and vegetables Determination of Chlorophyll or soluble sugar.
Edible fungi residue of the invention is flat mushroom bacteria residue or mushroom bacteria residue.
Edible fungi residue of the invention is, for example, to be obtained from Sanmenxia Gorge Citroen zx edible mushroom Specialty Co-operative Organization production flat mushroom
Flat mushroom bacteria residue, the mushroom bacteria residue obtained from Sanmenxia Gorge Lv Zhiyuan bio tech ltd production mushroom.
Described edible fungi residue is carrying out peelling off outer membrane using preceding needs, and comminution pretreatment collects granularity 80-
The edible fungi residue of 100 mesh.
The amino acid chelated calcium preparation method that the present invention is used is as follows：80 DEG C of temperature is heated water in a kettle., is pressed
According to compound amino acid and calcium salt part mol ratio 1：2, toward addition compound amino acid and calcium salt in water, are completely dissolved, then that its is molten
The pH of liquid is adjusted to 8.0, and chelatropic reaction 60min, then concentrated, dry, crushing are carried out under temperature 70 C, obtains amino acid chela
Close calcium.
Described calcium salt is, for example, calcium nitrate, naturally it is also possible to which using can be with other calcium salt of chelating amino acids, these
Calcium salt is also within the scope of the present invention.
Zinc-amino acid chelate be according to amino acid chelated calcium preparation method identical method prepare, simply pH 7.0,
The mol ratio of 35 DEG C of chelatropic reaction temperature, reaction time 30min, addition compound amino acid and zinc salt part is 2：1.
Described zinc salt is, for example, white vitriol, naturally it is also possible to using can with other zinc salts of chelating amino acids,
These zinc salts are also within the scope of the present invention.
The composite aminophenol powder that the present invention is used is the product sold in the market, for example, chelate biotechnology by Chengdu
Believe that chemical industry is limited in Co., Ltd, Chemical Co., Ltd. of Xinhua of Sichuan Mianzhu, Shanghai Yi He bio tech ltd, Xinxiang City
Company, Qi County of Shanxi reach Chemical Co., Ltd., Zhengzhou Kai Lan material for water treatment Co., Ltd with power Chemical Co., Ltd., Zhengzhou profit
The product of sale.
DA-6 is DA-6 diethyl aminoethyl hexanoates, and chemical name diethylamino ethanol caproate, pure white mealy crystal is soluble in water.
It is broad spectrum activity plant growth regulator, promotes cell division and growth, the differentiation of tachyauxesis point to improve leaf in plant body
The activity of green element, protein, nucleic acid content and photosynthetic rate, raising peroxidase and nitrate reductase, the carbon of promotion plant,
Nitrogen metabolism, enhancing plant pair liquid manure absorb and dry accumulation, water balance in regulation plant, enhancing crop, fruit tree
Disease-resistant, drought resisting, cold tolerance；Delay plant senesecence, promote early ripening of the crops, volume increase, improve crop and fruit quality, produce are made in raising
Amount.
Main Functions of the DA-6 in geobiont repair agent of the present invention is to strengthen improve fertilizer efficiency, improve crop and fruit product
Matter, improves crop yield.The DA-6 that the present invention is used is the product sold in the market, such as by Zhengzhou Zheng Shi chemical products
Co., Ltd, Zhengzhou brilliance agricultural science and technology Co., Ltd, Zhengzhou Zhong Ke chemical products Co., Ltd, Zhengzhou letter connection biochemical technology have
Limit the product of Company.
In geobiont repair agent of the invention, when the content of other components is in described scope, if the wax
The amount of sample bacillus bacterium powder is less than 1 weight portion, then be unfavorable for producing antibacterial material, it is impossible to suppress harmful microbe breeding and degraded
Harmful components in soil；If the wax-shaped bacillus bacterium powder amount is more than 2 weight portions, it is unfavorable in the geobiont repair agent
Other species microbial growths；Therefore, the amount of the wax-shaped bacillus bacterium powder for 1-2 weight portions be it is feasible, preferably
1.2-1.8 weight portions, more preferably 1.5 weight portion.
Similarly, when the content of other components is in described scope, if the pythium oligandrum fermentate amount is less than 6 weights
Part is measured, then pythium oligandrum bacterium can not colonize in crop rhizosphere well；If the pythium oligandrum fermentate amount is more than 12 weight
Part, then can not be compounded with optimal proportion with Trichoderma viride, so as to optimal disease resisting effect can not be played；Therefore, the wax-shaped bacillus
Bacterium powder amount is feasible, preferably 8-10 weight portions for 6-12 weight portions, more preferably 9 weight portion.
It is green if the Trichoderma viride bacterium powder amount is less than 6 weight portions when the content of other components is in described scope
Color Trichoderma can not colonize in crop rhizosphere well；If the Trichoderma viride bacterium amount is more than 12 weight portions, can not be with widow
Male rotten mould fermentate is compounded with optimal proportion, so as to optimal disease resisting effect can not be played；Therefore, the Trichoderma viride bacterium powder amount is
6-12 weight portions are feasible, preferably 8-10 weight portions, more preferably 9 weight portion.
Similarly, when the content of other components is in described scope, if the Sphingobacterium multivorum fermentate
Amount is less than 4 weight portions, then can not form dominant microflora in roots of crops, causes fiber degradation DeGrain；If described many
Food Sphingobacterium fermentate amount is more than 8 weight portions, then be unfavorable for the life of other kind quasi-microorganisms in the geobiont repair agent
Breeding long；Therefore, the Sphingobacterium multivorum fermentate amount for 4-8 weight portions be feasible, preferably 5-7 weight portions,
More preferably 6 weight portion.
When the content of other components is in described scope, if the edible fungi quantity of slag is less than 50 weight portions, no
Beneficial to microbial growth in geobiont repair agent；If the edible fungi quantity of slag is more than 60 weight portions, can cause
Beneficial microbe ratio is reduced in unit mass microbial inoculum；Therefore, the edible fungi quantity of slag for 50-60 weight portions be it is feasible,
Preferably 52-58 weight portions, more preferably 54-56 weight portions.
When the content of other components is in described scope, if the amino acid chelated calcium amount is less than 7 weight portions, no
Crops can be met in growth course to the demand of calcium constituent；If the amino acid chelated calcium amount is more than 15 weight portions,
Micro- proportional imbalance in other can be caused；Therefore, the amino acid chelated calcium amount for 7-15 weight portions be it is feasible, preferably
Ground is 9-13 weight portions, more preferably 10-12 weight portions.
When the content of other components is in described scope, if the zinc-amino acid chelate amount is less than 4 weight portions, no
Crops can be met in growth course to the demand of Zn-ef ficiency；If the zinc-amino acid chelate amount is more than 10 weight portions,
Micro- proportional imbalance in other can be caused；Therefore, the zinc-amino acid chelate amount for 4-10 weight portions be it is feasible, preferably
Ground is 5-9 weight portions, more preferably 6-8 weight portions.
When the content of other components is in described scope, if the amount of the DA-6 is less than 0.10 weight portion, can not
The accumulation of crop C N metabolism and dry is effectively promoted, if the amount of the DA-6 is more than 0.50 weight portion.Can then produce
Poisoning, therefore, the amount of the DA-6 is feasible, preferably 0.20-0.40 weight portions for 0.10-0.50 weight portions, more excellent
Selection of land is 0.28-0.32 weight portions.
Preferably, geobiont repair agent of the present invention is few male by 1.2-1.8 weight portion wax-shaped bacillus bacterium powder, 8-10 weight portions
Pythium spp powder, 8-10 weight portion Trichoderma virides bacterium powder, 5-7 weight portion Sphingobacterium multivorums fermentate, 52-58 weight portions eat
Bacterium bacteria residue, 9-13 weight portions amino acid chelated calcium, 5-9 weight portions zinc-amino acid chelate and 0.20-0.40 weight portions DA-6 composition.
It is highly preferred that geobiont repair agent of the present invention is by 1.5 weight portion wax-shaped bacillus bacterium powders, 9 weight portion pythium oligandrums
Bacterium powder, 9 weight portion Trichoderma viride bacterium powders, 6 weight portion Sphingobacterium multivorum fermentates, 54-56 weight portions edible fungi residue,
10-12 weight portions amino acid chelated calcium, 6-8 weight portions zinc-amino acid chelate and 0.28-0.32 weight portions DA-6 composition.
Geobiont repair agent preparation method of the present invention is as follows：
Method as described above prepares Sphingobacterium multivorum culture；
Method as described above prepares amino acid chelated calcium and zinc-amino acid chelate；
Method treatment edible fungi residue as described above；
By Sphingobacterium multivorum culture, amino acid chelated calcium, zinc-amino acid chelate, edible fungi residue, wax-shaped bacillus
The weight ratio of bacterium powder, pythium oligandrum bacterium powder, Trichoderma viride bacterium powder with DA-6 as described above is mixed, and obtains soil of the invention
Earth bioremediation agents.
Detected using the detection method that will be discussed in more detail below and determined, effective viable bacteria of geobiont repair agent of the present invention
>=0.50 hundred million/g of number.
Colorimetric method for determining geobiont repair of the present invention is used according to agricultural microbial agent (GB 20287-2006) standard
Cellulase activity >=the 90U/g of agent.
Colorimetric method for determining geobiont repair of the present invention is used according to agricultural microbial agent (GB 20287-2006) standard
Protease activity >=the 30U/g of agent.
According to the soil conditioner calcium of NY/T 2272, magnesium, the Determination of Silicon Content, geobiont repair agent of the present invention is determined
CaO content by weight >=0.50%.
According to copper and iron MnZn boron molybdenum content assay method in complex fertilizer, using GB/T 14540-20033.8 Atomic absorptions
Spectrophotometry, determines the Zn content of geobiont repair agent of the present invention by weight >=0.10%.
According to the fineness of agricultural microbial agent (GB 20287-2006) standard test geobiont repair agent of the present invention
(≤3mm) >=70.0%.
PH value according to agricultural microbial agent (GB 20287-2006) standard test geobiont repair agent of the present invention is
5.5~8.5.
Moisture according to agricultural microbial agent (GB 20287-2006) standard test geobiont repair agent of the present invention contains
Amount is by weight≤35%.
In the present invention, by wax-shaped bacillus bacterium powder, pythium oligandrum fermentate, Trichoderma viride bacterium powder, Sphingobacterium multivorum
Fermentate, edible fungi residue, amino acid chelated calcium, zinc-amino acid chelate and DA-6 compare 1-2 according to weight：6-12：6-12：4-8：
50-60：7-15：4-10：0.1-0.5 is well mixed, and obtains geobiont repair agent of the invention.
Description below is according to " agricultural microbial agent (GB 20287-2006) " standard detection Bacillus cereus, few hero
The method of rotten mould, Trichoderma viride and Sphingobacterium multivorum viable count.
Geobiont repair agent viable count of the present invention is detected using colony counting method, in detection Bacillus cereus and many foods
Nutrient agar is used during Sphingobacterium viable count, is added using Meng when pythium oligandrum and Trichoderma viride viable count is detected
Draw red culture medium.
Specific detecting step is as follows：
I, it is serially diluted：10g samples are weighed, is added to during 100mL has a sterilized water of bead, 20min is stood, rotary
30min is fully vibrated with rotating speed 200r/min on shaking table, mother liquor bacteria suspension (basal liquid) is obtained.Drawn with Sterile pipette
5.0mL mother liquor bacteria suspensions are added separately in 45mL sterilized waters, then according to volume ratio 1:10 are serially diluted, and are respectively obtained dilute
Degree of releasing is respectively 1:1×101、1:1×102、1:1×103、1:1×104... dilution bacteria suspension.
II, sample-adding and culture：Each sample takes 3 serial dilution degree, draws 0.1mL differences respectively with Sterile pipette dilute
Degree of releasing bacteria suspension, is added on previously prepared solid medium flat board, is respectively hanged different dilution factor bacterium with sterile glass scraper
Liquid is equably applied to agar surface.Each dilution factor is repeated 3 times, while blank is made with sterilized water, Sphingobacterium multivorum,
Bacillus cereus is at 37 DEG C of temperature, Trichoderma viride and pythium oligandrum are cultivated at 28 DEG C of temperature.
III, bacterium colony identification：According to detected pythium oligandrum bacterium, each dilution factor takes the different types of bacterium colony that represents and passes through
The conventional technical means such as smear, dyeing, microscopy confirm the validity bacterium.
Colony counting：With occur 20~300 flat boards of the dilution factor of clump count as counting criteria (filamentous fungi be 10~
150 clump counts), living bacteria count mesh and miscellaneous bacteria number are counted respectively.When only one of which dilution factor, its average colony number is 20
At~300, then calculated with the average colony number.If there is two dilution factors, its average colony number is between 20~300
When, should be determined by the ratio of both total plate counts.If its ratio should calculate both average less than or equal to 2；If more than if 2 with
The small bacterium colony average of dilution factor is calculated.Living bacteria count is calculated according to following formula (1)：
n m=xkv1/(m 0v2)x10-8………(1)
In formula：
n mRepresentation quality living bacteria count, hundred million/g；
X represents bacterium colony average, individual；
K represents extension rate；
v2Represent bacteria suspension addition, mL；
m0Representative sample amount, g.
Geobiont repair agent of the invention can using spreading manuer in holes, ditch spread or plough after spreading fertilizer over the fields, also can be used to chase after as base manure
Fertilizer is applied.Those skilled in the art can determine the administration of geobiont repair agent of the present invention according to practical condition
Amount and application process, this does not exist any technical problem, is easily achieved for those skilled in the art.
[beneficial effect]
The beneficial effects of the invention are as follows：
Geobiont repair agent of the present invention contains a large amount of beneficial activity bacterium and metabolic enzyme, and animals and plants are residual in decomposing soil
Body, feces of livestock and poultry, improve crop rhizosphere soils microenvironment, being capable of chesson, alleviation soil hardening and salinization of soil, promotion farming
Thing root development, suppression harmful microorganism activity, reduces soil-borne disease and occurs, and reduces Pesticide use amount, strengthens the anti-of crops
Inverse property, and then improve crop yield and quality；There is repair of degrading strongly to " oil-agriculture " pollution and residues of pesticides simultaneously；
Trace element can alleviate hunger sign in plant in Chelating state in product, mitigate or remove heavy metal in soil pollution, improve chemical fertilizer
Utilization rate, reduces the harm that brings of excessive fertilization, is conducive to economizing on resources, environmental protection.
【Brief description of the drawings】
Fig. 1 is the apple production figure of different disposal.
【Specific embodiment】
The present invention is will be better understood that by following embodiments.
Embodiment 1：It is prepared by geobiont repair agent of the present invention
The embodiment is implemented in the following manner：
First, prepare Sphingobacterium multivorum fermentate
A, activation culture
Prepare beef extract-peptone fluid nutrient medium：5g beef extracts, 10g peptones, 10g glucose, 5g NaCl are added to
In 1000ml distilled water, boil and dissolve, then sterilize 30min at 121 DEG C of temperature, obtains beef extract-peptone Liquid Culture
Base；
Under sterile conditions, 0.1g Sphingobacterium multivorums are inoculated into the above-mentioned beef extract-peptone liquid trainings of 200mL
Support in base, 48h is cultivated under conditions of 30 DEG C of temperature with rotating speed 180r/min, obtain Sphingobacterium multivorum activation culture thing；
B, strain purification
5.0g beef extracts, 10.0g peptones, 5.0g sodium chloride and 18-20g nutrient agars are added in 1000ml water, it is molten
Solution, then sterilize 30min at 121 DEG C of temperature, obtains beef extract-peptone solid medium.
Under sterile conditions, in the Sphingobacterium multivorum activation culture thing for being obtained from step A using aseptic inoculation ring
The a small amount of culture of picking is added in beef-protein medium flat board, line, permanent at 30 DEG C of temperature in the constant incubator
Temperature culture 48h, obtains pure Sphingobacterium multivorum culture；
C, seed liquor culture
Prepare seed liquid culture medium：6g MgSO4.·7H2O, 6g NaCl, 20g bean cake powder are well mixed with 10g corn flour,
1000mL dissolvings are added water to, the pH of its solution is adjusted to 7.1, then sterilize 30min at 121 DEG C of temperature, obtains seed liquor
Culture medium.
From the single Sphingobacterium multivorum colony inoculation of picking on the beef-protein medium flat board that step B is obtained to
In above-mentioned seed liquid culture medium, the concussion and cultivate 36h under conditions of 30 DEG C of temperature with rotating speed 220r/min obtains many food sphingols
Bacillus inoculum；
D, fermented and cultured
Wheat bran, corn flour, bean cake powder compare 1 according to weight：0.5：0.5 be well mixed, add water regulation to water content be with
Weight meter 40%, sterilizing, obtains solid fermentation culture medium；
Then, it is many food sphingol bars for obtaining step C 8% in terms of solid fermentation culture medium weight according to inoculum concentration
Bacterium inoculum is inoculated into the solid fermentation culture medium, and solid fermentation culture 4d is carried out under conditions of 28 DEG C of temperature,
The solid fermentation cultured products that obtain then carry out machinery turn, the low temperature drying under conditions of 30 DEG C of temperature, crush, obtain institute
The Sphingobacterium multivorum fermentate stated.
Second, prepare amino acid chelated calcium：80 DEG C of temperature is heated water in a kettle., according to compound amino acid and calcium
Salt part mol ratio 1：2 toward the compound amino acid sold by Chemical Co., Ltd. of Xinhua of Sichuan Mianzhu is added in water, by Xinxiang City
The calcium nitrate of Zhong Xin Chemical Co., Ltd.s sale, is completely dissolved, and then adjusts to 8.0, under temperature 70 C the pH of its solution
Chelatropic reaction 60min, then concentrated, dry, crushing are carried out, amino acid chelated calcium is obtained.
40 DEG C of temperature is heated water in a kettle., according to compound amino acid and zinc salt part mol ratio 2：1 toward in water
The composite aminophenol powder sold by Chemical Co., Ltd. of Xinhua of Sichuan Mianzhu is added, is sold up to Chemical Co., Ltd. by Zhengzhou profit
White vitriol, is completely dissolved, and then adjusts to 7.0 the pH of its solution, and chelatropic reaction 30min is carried out at 35 DEG C of temperature,
Concentrated, dry again, crushing, obtains zinc-amino acid chelate.
3rd, flat mushroom bacteria residue outer membrane is peelled off, crushed using common pulverizer, collect the flat mushroom of granularity 80-100 mesh
Edible fungi residue.
4th, the Sphingobacterium multivorum fermentate of above-mentioned preparation, the wax of Jiangsu Lv Ke Bioisystech Co., Ltd sale
Sample bacillus bacterium powder, the pythium oligandrum bacterium powder of In Henan Agriculture microbial engineering research center production, Yiyuan member of Imperial Academy agricultural hair
Exhibition Co., Ltd sale Trichoderma viride bacterium powder, above-mentioned pretreatment flat mushroom edible fungi residue, the amino acid chelated calcium of above-mentioned preparation,
Zinc-amino acid chelate and the DA-6 of Zhengzhou Zheng Shi Chemical Co., Ltd.'s sale, 4 are compared according to weight：1：12：12：50：7：10：
0.5 is well mixed, and obtains geobiont repair agent of the present invention.
Detected using the method described in this specification, geobiont repair agent manufactured in the present embodiment it is total effectively
1.1 hundred million/g of viable count, cellulase activity 148U/g, protease activity 65U/g, calcium content are by weight 0.58%, Zn content is with weight
Gauge 0.35%, fineness (≤3mm) 85%, pH 6.5, water content by weight 22.2%.
Embodiment 2：It is prepared by geobiont repair agent of the present invention
The embodiment is implemented in the following manner：
First, prepare Sphingobacterium multivorum fermentate
A, activation culture
Beef extract-peptone fluid nutrient medium is prepared according to the same manner as in Example 1.
Under sterile conditions, 0.1g Sphingobacterium multivorums are inoculated into the above-mentioned beef extract-peptone liquid trainings of 200mL
Support in base, 48h is cultivated under conditions of 30 DEG C of temperature with rotating speed 180r/min, obtain Sphingobacterium multivorum activation culture thing；
B, strain purification
Beef extract-peptone solid medium is prepared according to the same manner as in Example 1, and carries out strain purification.
C, seed liquor culture
Seed liquid culture medium is prepared according to the same manner as in Example 1.
From the single Sphingobacterium multivorum colony inoculation of picking on the beef-protein medium flat board that step B is obtained to
In above-mentioned seed liquid culture medium, the concussion and cultivate 36h under conditions of 30 DEG C of temperature with rotating speed 220r/min obtains many food sphingols
Bacillus inoculum；
D, fermented and cultured
Wheat bran, corn flour, bean cake powder compare 4.5 according to weight：2：1.5 be well mixed, add water regulation to water content be with
Weight meter 50%, sterilizing, obtains solid fermentation culture medium；
Then, it is many food sphingol bars for obtaining step C 10% in terms of solid fermentation culture medium weight according to inoculum concentration
Bacterium inoculum is inoculated into the solid fermentation culture medium, and solid fermentation culture 3d is carried out under conditions of 35 DEG C of temperature,
The solid fermentation cultured products that obtain then carry out machinery turn, the low temperature drying under conditions of 38 DEG C of temperature, crush, obtain institute
The Sphingobacterium multivorum fermentate stated.
Secondth, amino acid chelated calcium and zinc-amino acid chelate are prepared according to the same manner as in Example 1.
3rd, mushroom bacteria residue outer membrane is peelled off, crushed using common pulverizer, collect the mushroom of granularity 80-100 mesh
Edible fungi residue.
4th, sold by the Sphingobacterium multivorum fermentate of above-mentioned preparation, by Cangzhou Wang Fa Bioisystech Co., Ltd
Wax-shaped bacillus bacterium powder, the pythium oligandrum bacterium powder, green by Guangzhou that is produced by In Henan Agriculture microbial engineering research center
Trichoderma viride bacterium powder, the mushroom edible fungi residue of above-mentioned pretreatment, the ammonia of above-mentioned preparation of brightness bio tech ltd sale
Base acid chelating calcium, zinc-amino acid chelate and the DA-6 sold by Zhengzhou brilliance agricultural science and technology Co., Ltd, 7 are compared according to weight：
1.8：6：6：60：15：4：0.1 mixing, obtains geobiont repair agent of the invention.
Detected using the method described in this specification, geobiont repair agent manufactured in the present embodiment it is total effectively
1.4 hundred million/g of viable count, cellulase activity 162U/g, protease activity 73U/g, calcium content are by weight 0.85%, Zn content is with weight
Gauge 0.18%, fineness (≤3mm) 86%, pH 6.5, water content by weight 23.8%.
Embodiment 3：It is prepared by geobiont repair agent of the present invention
The embodiment is implemented in the following manner：
First, prepare Sphingobacterium multivorum
A, activation culture
Beef extract-peptone fluid nutrient medium is prepared according to the same manner as in Example 1.
Under sterile conditions, 0.1g was connect by the Sphingobacterium multivorum that bio tech ltd of upper Haifeng county longevity provides
Kind in the above-mentioned beef extract-peptone fluid nutrient mediums of 200mL, cultivated under conditions of 30 DEG C of temperature with rotating speed 180r/min
48h, obtains Sphingobacterium multivorum activation culture thing；
B, strain purification
The preparation of beef extract-peptone solid medium is carried out according to the same manner as in Example 1, and carries out strain carrying
It is pure.
C, seed liquor culture
Seed liquid culture medium is prepared according to the same manner as in Example 1.
From the single Sphingobacterium multivorum colony inoculation of picking on the beef-protein medium flat board that step B is obtained to
In above-mentioned seed liquid culture medium, the concussion and cultivate 36h under conditions of 30 DEG C of temperature with rotating speed 220r/min obtains many food sphingols
Bacillus inoculum；
D, fermented and cultured
Wheat bran, corn flour, bean cake powder compare 2 according to weight：1：1.2 are well mixed, and it is with weight to add water regulation to water content
Gauge 44%, sterilizing, obtains solid fermentation culture medium；
Then, it is many food sphingol bars for obtaining step C 9% in terms of solid fermentation culture medium weight according to inoculum concentration
Bacterium inoculum is inoculated into the solid fermentation culture medium, and solid fermentation culture 4d is carried out under conditions of 32 DEG C of temperature,
The solid fermentation cultured products that obtain then carry out machinery turn, the low temperature drying under conditions of 35 DEG C of temperature, crush, obtain institute
The Sphingobacterium multivorum fermentate stated.
Secondth, amino acid chelated calcium and zinc-amino acid chelate are prepared according to the same manner as in Example 1.
3rd, flat mushroom bacteria residue outer membrane is peelled off, crushed using common pulverizer, collect the flat mushroom of granularity 80-100 mesh
Edible fungi residue.
4th, sold by the Sphingobacterium multivorum fermentate of above-mentioned preparation, by Henan Yangshao Biochemical Engineering Co., Ltd.
Wax-shaped bacillus bacterium powder, the pythium oligandrum bacterium powder, long by Shandong that is produced by In Henan Agriculture microbial engineering research center
Trichoderma viride bacterium powder, the flat mushroom edible fungi residue of above-mentioned pretreatment, the ammonia of above-mentioned preparation of safe bio tech ltd's sale
Base acid chelating calcium, zinc-amino acid chelate and the DA-6 sold by Zhengzhou Zhong Ke chemical products Co., Ltd, 8 are compared according to weight：2：
10：8：55：12：6：0.3 is well mixed, and obtains geobiont repair agent of the invention.
Detected using the method described in this specification, geobiont repair agent manufactured in the present embodiment it is total effectively
1.3 hundred million/g of viable count, cellulase activity 172U/g, protease activity 78U/g, calcium content are by weight 0.72%, Zn content is with weight
Gauge 0.28%, fineness (≤3mm) 88%, pH 6.8, water content by weight 22.5%.
Embodiment 4：It is prepared by geobiont repair agent of the present invention
The embodiment is implemented in the following manner：
First, prepare Sphingobacterium multivorum
A, activation culture
Beef extract-peptone fluid nutrient medium is prepared according to the same manner as in Example 1.
Under sterile conditions, the Sphingobacterium multivorum inoculation for 0.1g Shanghai Chun Shi Bioisystech Co., Ltd being provided
To in the above-mentioned beef extract-peptone fluid nutrient mediums of 200mL, 48h is cultivated under conditions of 30 DEG C of temperature with rotating speed 180r/min,
Obtain Sphingobacterium multivorum activation culture thing；
B, strain purification
Beef extract-peptone solid medium is prepared according to the same manner as in Example 1, and carries out strain purification.
C, seed liquor culture
Seed liquid culture medium is prepared according to the same manner as in Example 1.
From the single Sphingobacterium multivorum colony inoculation of picking on the beef-protein medium flat board that step B is obtained to
In above-mentioned seed liquid culture medium, the concussion and cultivate 36h under conditions of 30 DEG C of temperature with rotating speed 220r/min obtains many food sphingols
Bacillus inoculum；
D, fermented and cultured
Wheat bran, corn flour, bean cake powder compare 3 according to weight：1.5：1 is well mixed, and it is with weight to add water regulation to water content
Gauge 48%, sterilizing, obtains solid fermentation culture medium；
Then, it is many food sphingol bars for obtaining step C 9% in terms of solid fermentation culture medium weight according to inoculum concentration
Bacterium inoculum is inoculated into the solid fermentation culture medium, and solid fermentation culture 3d is carried out under conditions of 33 DEG C of temperature,
The solid fermentation cultured products that obtain then carry out machinery turn, the low temperature drying under conditions of 32 DEG C of temperature, crush, obtain institute
The Sphingobacterium multivorum fermentate stated.
Second, amino acid chelated calcium and zinc-amino acid chelate are prepared according to the same manner as in Example 1.
3rd, mushroom bacteria residue outer membrane is peelled off, crushed using common pulverizer, collect the mushroom of granularity 80-100 mesh
Edible fungi residue.
4th, sold by the Sphingobacterium multivorum fermentate of above-mentioned preparation, by Wuhan Bioisystech Co., Ltd of Toyota
Wax-shaped bacillus bacterium powder, by In Henan Agriculture microbial engineering research center produce pythium oligandrum bacterium powder, by Yiyuan health
Trichoderma viride bacterium powder, the mushroom edible fungi residue of above-mentioned pretreatment, the ammonia of above-mentioned preparation of source bio tech ltd sale
Base acid chelating calcium, zinc-amino acid chelate and the DA-6 sold by Zhengzhou Xin Lian biochemical technologies Co., Ltd, 6 are compared according to weight：
1.5：8：10：58：8：9：0.4 is well mixed, and obtains geobiont repair agent of the invention.
Detected using the method for this specification description, total effective work of geobiont repair agent manufactured in the present embodiment
1.5 hundred million/g of bacterium number, cellulase activity 172U/g, protease activity 74U/g, calcium content are by weight 0.62%, Zn content is with weight
Meter 0.31%, fineness (≤3mm) 88%, pH 6.7, water content by weight 23.6%.
Test example 1：The apple tree growth condition experiment of geobiont repair agent of the present invention
Experiment kind：10 years raw red Fuji apple trees.
Test period：Field information experiment was carried out 2 years at -2014 years 2013, and track within 1 year.
Experimental field overview：Yantai City Mouping District, soil types is brown earth, and top layer quality is light earth, and fertility is uniform, irrigation and drainage bar
Part is good, and fertilizer test was not carried out in the past.
Treatment 1：Its soil conditioner is ploughed under according to every mu of 60kg crowd's moral board soil conditioner in tree crown area in mid-March
Underground, other agronomic measures and field management are performed according to local high quality apple production specifications.
Treatment 2：Using the geobiont repair agent of the present invention with 1 equivalent for the treatment of, other agronomic measures and field management are equal
Performed according to local high quality apple production specifications.
Fruit tree and fruit appearance change are observed during testing.Several harvests after fruit maturation, start to adopt at the beginning of 10 months then
Receive, in mid-November, harvesting terminates.It is respectively experimental field single receive, it is single claim, Dan Jichong, count each experimental field harvesting yield every time,
And randomly select 200 apples and carry out pol and acidity assay.
Two pedotheques of different disposal are being gathered by the end of November, and assay is being carried out to pH value.
Result of the test：Different disposal is listed in Table 1 below on apple quality influence result of the test.
Table 1：Apple sugar content and acidity result of laboratory test
The result of table 1 clearly shows that geobiont repair agent pol of the present invention improves 0.8%, and acidity reduction by 0.03% changes
Kind apple mouthfeel, improves fruit quality.
Different disposal is residual on apple agriculture and heavy metal degraded influence result of the test is listed in Table 2 below.
Table 2：The important residues of pesticides of apple and heavy metal analysis
The result of table 2 clearly shows that Fenpropathrin, fenvalerate residual and each heavy metal species are residual before experiment and in treatment 1
Though allowance is in limit range, show higher, geobiont repair agent of the present invention can effectively reduce Fenpropathrin, the chrysanthemum of cyanogen penta
Ester is remained and heavy-metal residual.As can be seen here, can be in degrading pesticide residues, fixing soil using the geobiont rehabilitation agent
The pollutants such as heavy metal, can rehabilitating soil, finally ensure food security.
Different disposal is shown in accompanying drawing 1 to apple production influence result of the test.
Be can be seen that by accompanying drawing 1, geobiont repair agent mu volume increase 221.2kg of the present invention, rate of growth is 7.10%.Thus may be used
See, apple production is remarkably improved using described geobiont repair agent.
Different disposal is listed in Table 3 below on apple economic benefit influence result of the test.
Table 3：Influence of the different disposal to apple economic benefit
Note：Apple unit price is 6 yuan/kg.Amount to yuan/mu of expense 180 for examination fertilizer and labour cost.
The result of table 3 clearly shows, 1147.2 yuan of geobiont repair agent mu increment of the present invention, and input-output ratio is 1:
16.59.As can be seen here, apple economic benefit can be improved using described geobiont repair agent.
Different soils conditioner is listed in Table 4 below to soil physical chemistry performance impact result of the test.
Table 4：Influence of the different disposal to soil physical chemistry performance
From the graph as can be seen that geobiont repair agent of the present invention reduces the soil weight, the content of organic matter is improve, had
Certain dissolving phosphor and dissolving potassium effect, improves chemical fertilizer utilization ratio, reduces waste.As can be seen here, repaiied using described geobiont
Multiple agent can build soil good environment with improved soil nodule structure, strengthen soil water-reataining fertilizer-keeping ability, be favorably improved micro- life
Thing vigor.
Geobiont repair agent of the present invention can not only improve apple production and quality, and can reduce that agriculture is residual and a huge sum of money
Category；The soil weight is reduced, improves soil granular structure；There is certain dissolving phosphor and dissolving potassium to act on simultaneously, improve, chemical fertilizer utilization ratio；Increase
Strong soil water-reataining fertilizer-keeping ability is favorably improved microbial activity.
Test example 2：The tomato cultivation application test of geobiont repair agent of the present invention
Experiment kind：No. four tomatoes of Gui powder
Test site：In Qing Heji villages of Cao Gang townshiies greenhouse
Test period：In April, 2013 is in August, 2015
Test method：This experiment sets 4 treatment, 3 repetitions, using randomized complete-block design, plot area 40m2.Make
For base manure in transplant before ridging when disposable row replacement soil ridge body 20cm depths, transplanting 0.6 meter of line-spacing, 0.4 meter of spacing in the rows, Transplanting Density
2778 plants/acre.
Treatment 1：Conventional fertilizer application 80kg/ mus (20kg urea, 10kg MAPs and 50kg potassium sulfates), other maintenance measures
Performed according to local tomato planting technical specification.
Treatment 2：80kg/ mus of geobiont repair agent of the present invention is enriched on the basis for the treatment of 1, other maintenance measures are pressed
Performed according to local tomato planting technical specification.
Treatment 3：The base that 80kg/ mus of geobiont repair agent microorganism of the present invention is inactivated is enriched on the basis for the treatment of 1
Matter, other maintenance measures are performed according to local tomato planting technical specification.
Treatment 4：Blank
Different disposal is listed in Table 5 below to tomato biological affect trait result of the test.
Table 5：Influence of the different disposal to tomato biological proterties
| Treatment | Leaf color | Fruit color | Fruit type | Per plant (individual) | Single fruit weight (gram) |
| 1 | It is grayish green | Pink shoulder is green | Slightly fruitlet and malformed fruit | 14 | 171.9 |
| 2 | It is dark green | Pink light | Neat and consistent | 16 | 175.3 |
| 3 | It is grayish green | Pink shoulder is green | Slightly fruitlet and malformed fruit | 14 | 172.8 |
| 4 | It is light green | Pink shoulder is green | It is irregular | 13 | 156.5 |
The result listed from table 5 can be seen that and use No. four tomato single fruit weights of Gui powder of geobiont repair agent of the present invention
Relatively treatment 1, treatment 3 and treatment 4 increase by 3.4 grams, 2.5 grams and 18.8 grams respectively, improve fruit type, so as to improve yield and commodity
Property.
Different disposal is listed in Table 6 below to tomato disease resistance result of the test.
Table 6：Influence of the different disposal to tomato florescence disease resistance
Wherein：
Tomato mosaic disease disease index grade scale is as follows：
0 grade：Complete stool is disease-free；
1 grade：Complete stool 1/4 has a small number of scabs with lower blade；
2 grades：Complete stool 1/2 has a small amount of scab or 1/4 has more scab with lower blade with lower blade；
3 grades：Complete stool 3/4 is fallen ill with lower blade or complete stool 1/4 is withered and yellow with lower blade full leaf；
4 grades：Complete stool 3/4 fallen ill with blade or complete stool 1/2 with lower blade it is withered and yellow~whole strain is withered and yellow.
Tomato root-knot eelworm disease index grade scale is as follows：
0 grade be root system without insect gall；
1 grade：Secondary root butt has small insect gall, accounts for less than the 10% of total root system；
2 grades：Lateral root butt forms insect gall, accounts for the 10%~30% of lateral root sum；
3 grades：Main root butt forms insect gall, has insect gall lateral root number to account for the 30%~70% of sum；
4 grades：Root growth fibrous root group, has insect gall lateral root number to account for the 70%~100% of sum.
The result of table 6 clearly shows that geobiont repair agent of the present invention processes the disease in tomato florescence Major Diseases
Strain rate and disease refer to minimum, and microorganisms with specific functions has antagonism to pathogen in showing geobiont repair agent of the present invention,
Suppress and eliminated disease source to a certain extent, be conducive to improving tomato plant disease resistance.
Different disposal is shown in Table 7 on Tomato Quality influence result of the test.
Table 7：Influence of the different disposal to Tomato Quality
| Treatment | Soluble sugar % | Soluble solid % | Total acid % | Vitamin C (mg/100g) |
| 1 | 4.44 | 4.59 | 0.44 | 14.09 |
| 2 | 4.78 | 4.72 | 0.40 | 14.35 |
| 3 | 4.56 | 4.55 | 4.42 | 14.13 |
| 4 | 4.32 | 4.50 | 4.44 | 14.08 |
The result of table 7 clearly shows that geobiont repair agent of the present invention can improve soluble sugar, soluble solid, dimension
Raw element C content, reduces total acid content.All in all, optimal to process 2 performances, treatment 1 and 3 differences for the treatment of are not notable, thus may be used
See, be conducive to improving fruit mouthfeel using geobiont repair agent of the present invention, improve Tomato Quality.
Different disposal is listed in Table 8 below on tomato yield influence result of the test.
Table 8：The yield result statistical form of different disposal
The result of table 8 clearly shows that geobiont repair agent per mu yield of the present invention relatively processes 1, treatment 3 and increases production respectively
350kg and 512kg, rate of growth is respectively increased 6.1% and 9.1%.As can be seen here, geobiont repair agent of the invention no matter
Defending and fighting against diseases aspect, or be significantly increased in terms of increasing yield and improving quality.
Statistical analysis is carried out to the cell production listed in table 8 according to Excel2007 and DPS7.05, its analysis result
It is listed in table 5 and table 6.
Table 5：Cell production analysis of variance table
| Source of variation | Quadratic sum | The free degree | Variance | F | ||
| Between treatment | 1974.87 | 3 | 658.29 | 34.2** | 4.76 | 9.78 |
| Between district's groups | 6.04 | 2 | 3.02 | 0.16 | 5.14 | 10.92 |
| Chance error | 115.5 | 6 | 19.25 | |||
| Total variation | 2096.41 | 11 |
FBetween district's groups=0.16 ＜ F0.05Area's group difference is not notable；FBetween treatment=34.2 ＜ F0.01Difference is extremely notable between treatment.
The LSD Multiple range test tables of table 6
Above-mentioned statistic analysis result explanation：Volume variance reaches the pole level of signifiance between Analysis of variance, treatment.Entered with PISD methods
Row Multiple range test, significant difference between product of the present invention and treatment 1, treatment 3 and treatment 4.
As can be seen here, geobiont repair agent of the invention is no matter in terms of disease resistance, or in terms of increasing yield and improving quality
It is superior to conventional fertilizer application.
Claims (10)
1. a kind of geobiont repair agent, it is characterised in that the geobiont repair agent is by 1-2 weight portion wax-shaped bacillus bacterium powder, 6-
12 weight portion pythium oligandrum bacterium powders, 6-12 weight portion Trichoderma virides bacterium powder, 4-8 weight portion Sphingobacterium multivorums fermentate, 50-
60 weight portion edible fungi residues, 7-15 weight portions amino acid chelated calcium, 4-10 weight portions zinc-amino acid chelate and 0.10-0.50 weight
Amount part DA-6 compositions.
2. geobiont repair agent according to claim 1, it is characterised in that described bacillus cereus bacterium powder viable bacteria
>=200.0 hundred million/g of number, pythium oligandrum bacterium powder viable count >=10.0 hundred million/g, Trichoderma viride bacterium powder viable count >=10.0 hundred million/g and many foods
Sphingobacterium fermentate >=5.0 hundred million/g.
3. geobiont repair agent according to claim 1, it is characterised in that Sphingobacterium multivorum fermentate be according to
What following step was prepared：
A, activation culture
Prepare beef extract-peptone fluid nutrient medium：5g beef extracts, 10g peptones, 10g glucose, 5g NaCl are added to
In 1000ml distilled water, boil and dissolve, then sterilize 30min at 121 DEG C of temperature, obtains beef extract-peptone Liquid Culture
Base；
Under sterile conditions, 0.1g Sphingobacterium multivorums are inoculated into the above-mentioned beef extract-peptone fluid nutrient mediums of 200mL
In, 48h is cultivated under conditions of 30 DEG C of temperature with rotating speed 180r/min, obtain Sphingobacterium multivorum activation culture thing；
B, strain purification
Under sterile conditions, picking in the Sphingobacterium multivorum activation culture thing for being obtained from step A using aseptic inoculation ring
A small amount of culture is added in beef-protein medium flat board, is rule, the constant temperature training at 30 DEG C of temperature in the constant incubator
48h is supported, pure Sphingobacterium multivorum culture is obtained；
C, seed liquor culture
Prepare seed liquid culture medium：6g MgSO4.·7H2O, 6g NaCl, 20g bean cake powder are well mixed with 10g corn flour, add water
To 1000mL dissolvings, the pH of its solution is adjusted to 7.1, then sterilize 30min at 121 DEG C of temperature, obtains seed liquor culture
Base；
From the single Sphingobacterium multivorum colony inoculation of picking on the beef-protein medium flat board that step B is obtained to above-mentioned
In seed liquid culture medium, the concussion and cultivate 36h under conditions of 30 DEG C of temperature with rotating speed 220r/min obtains Sphingobacterium multivorum
Inoculum；
D, fermented and cultured
Wheat bran, corn flour, bean cake powder compare 1-4.5 according to weight：0.5-2：0.5-1.5 is well mixed, and adds water regulation to aqueous
It is 40-50%, sterilizing by weight to measure, and obtains solid fermentation culture medium；
Then, it is the 8-10% in terms of solid fermentation culture medium weight, the Sphingobacterium multivorum that step C is obtained according to inoculum concentration
Inoculum is inoculated into the solid fermentation culture medium, and solid fermentation culture 3- is carried out under conditions of 28-35 DEG C of temperature
4d, the solid fermentation cultured products for obtaining then carry out machinery turn, low temperature drying and crushing, obtain described many food sphingols
Bacillus fermentation thing.
4. geobiont repair agent according to claim 3, it is characterised in that in step D, the solid fermentation culture
Thing carries out low temperature drying under conditions of 30-38 DEG C of temperature.
5. geobiont repair agent according to claim 1, it is characterised in that described edible fungi residue is mushroom bacteria residue
Or flat mushroom bacteria residue.
6. geobiont repair agent according to claim 1, it is characterised in that described amino acid chelated calcium is under
State what preparation process was prepared：
80 DEG C of temperature is heated water in a kettle., according to compound amino acid and calcium salt part mol ratio 1：2 add toward in water
Compound amino acid and calcium salt, are completely dissolved, and then adjust to 8.0 the pH of its solution, and chelatropic reaction is carried out under temperature 70 C
60min, then concentrated, dry, crushing, obtain amino acid chelated calcium..
7. geobiont repair agent according to claim 1, it is characterised in that described zinc-amino acid chelate is under
State what preparation process was prepared：
40 DEG C of temperature is heated water in a kettle., according to compound amino acid and zinc salt part mol ratio 2：1 adds toward in water
Compound amino acid and zinc salt, are completely dissolved, and then adjust to 7.0 the pH of its solution, and chelatropic reaction is carried out at 35 DEG C of temperature
30min, then concentrated, dry, crushing, obtain zinc-amino acid chelate.
8. geobiont repair agent according to claim 1, it is characterised in that the geobiont repair agent is by 1.2~1.8
Weight portion wax-shaped bacillus bacterium powder, 8~10 weight portion pythium oligandrum fermentates, 8~10 weight portion Trichoderma viride bacterium powders, 5~7 weight
Part Sphingobacterium multivorum fermentate, 53~58 weight portion edible fungi residues, 9~13 weight portion amino acid chelated calciums, 6~9 weights
Amount part zinc-amino acid chelate and 0.20~0.40 weight portion DA-6 compositions.
9. soil according to claim 1, it is characterised in that the geobiont repair agent is by 1.5 weight portion wax-shaped bacillus bacterium
Powder, 9 weight portion pythium oligandrum bacterium powders, 9 weight portion Trichoderma viride bacterium powders, 6 weight portion Sphingobacterium multivorum fermentates, 54~56
Weight portion edible fungi residue, 10~12 weight portion amino acid chelated calciums, 6~8 weight portion zinc-amino acid chelates and 0.28~0.32
Weight portion DA-6 is constituted.
10. soil according to claim 1, it is characterised in that its total living bacteria count >=0.5 hundred million/g, cellulase activity
>=90.0U/g, protease activity >=30.0U/g, calcium content by weight >=0.50%, Zn content by weight >=0.10%, it is thin
Degree (≤3mm) >=70.0%, pH 5.5~8.5, water content is by weight≤35.0%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611173685.5A CN106831091A (en) | 2016-12-16 | 2016-12-16 | A kind of geobiont repair |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611173685.5A CN106831091A (en) | 2016-12-16 | 2016-12-16 | A kind of geobiont repair |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106831091A true CN106831091A (en) | 2017-06-13 |
Family
ID=59140356
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611173685.5A Pending CN106831091A (en) | 2016-12-16 | 2016-12-16 | A kind of geobiont repair |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106831091A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113578957A (en) * | 2021-08-02 | 2021-11-02 | 江苏省农业科学院 | Method for improving degradation rate of pyrimethanil serving as bactericide |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101890426A (en) * | 2010-07-19 | 2010-11-24 | 南开大学 | Method for restoring petroleum-polluted saline alkali soil by ryegrass and high efficiency microbes |
| CN102405936A (en) * | 2011-07-20 | 2012-04-11 | 蔡建国 | Novel anti-virus microorganism revivifier capable of resisting continuous cropping |
| CN103266143A (en) * | 2013-06-05 | 2013-08-28 | 河南工业大学 | Method for preparing compound amino acid chelated zinc by utilizing cold-pressed sesame-seed cake zymolyte |
| CN103787746A (en) * | 2014-01-10 | 2014-05-14 | 武汉盛大神农生物工程有限公司 | Preparation for reducing soil-borne disease, promoting rooting and resisting continuous cropping |
| CN106146194A (en) * | 2016-07-18 | 2016-11-23 | 三门峡龙飞生物工程有限公司 | A kind of anti-continuous cropping microbial inoculum |
-
2016
- 2016-12-16 CN CN201611173685.5A patent/CN106831091A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101890426A (en) * | 2010-07-19 | 2010-11-24 | 南开大学 | Method for restoring petroleum-polluted saline alkali soil by ryegrass and high efficiency microbes |
| CN102405936A (en) * | 2011-07-20 | 2012-04-11 | 蔡建国 | Novel anti-virus microorganism revivifier capable of resisting continuous cropping |
| CN103266143A (en) * | 2013-06-05 | 2013-08-28 | 河南工业大学 | Method for preparing compound amino acid chelated zinc by utilizing cold-pressed sesame-seed cake zymolyte |
| CN103787746A (en) * | 2014-01-10 | 2014-05-14 | 武汉盛大神农生物工程有限公司 | Preparation for reducing soil-borne disease, promoting rooting and resisting continuous cropping |
| CN106146194A (en) * | 2016-07-18 | 2016-11-23 | 三门峡龙飞生物工程有限公司 | A kind of anti-continuous cropping microbial inoculum |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113578957A (en) * | 2021-08-02 | 2021-11-02 | 江苏省农业科学院 | Method for improving degradation rate of pyrimethanil serving as bactericide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106947725B (en) | Microbial agent and application thereof in saline-alkali soil improvement | |
| CN102876576B (en) | Acidic soil conditioner, and preparation method and application thereof | |
| CN106495826A (en) | A kind of production method of biological bacteria fruit tree drip irrigation fertilizer | |
| CN111117910B (en) | Enterobacter ludwigii PN6 and application thereof | |
| CN104761382A (en) | Fermented ferment fertilizer, and preparation and using methods thereof | |
| CN104894010A (en) | Compound microbial fertilizer for antagonism of soil-borne fungal diseases, and preparation method and application thereof | |
| CN101497541B (en) | Efficient disease-resistant phosphate solubilizing bacterial manure for tobacco and production method thereof | |
| CN105503391B (en) | A kind of prevention and treatment tomato powdery mildew of cucumber function foliar fertilizer and application | |
| CN106977278A (en) | Potassium polyphosphate sugarcane slow-release fertilizer and its production method | |
| CN105964680B (en) | A kind of beach saline land continuous cropping cotton soil ecology renovation agent and the preparation method and application thereof | |
| Lavania et al. | Solubilization of tricalcium phosphate by temperature and salt tolerant Serratia marcescens NBRI1213 isolated from alkaline soils | |
| CN103304305A (en) | Compound-microorganism functional enteromorpha foliar fertilizer and preparation method thereof | |
| CN109504622A (en) | A kind of lysine bacillus and the composite bacteria agent of bacillus subtilis and preparation method thereof | |
| CN105801258A (en) | Bacillus subtilis fertilizer and preparation method and application thereof | |
| CN101402533A (en) | Insecticide fertilizer for tomato production based on biogas slurry concentrated liquor and process for producing biogas slurry concentrated liquor | |
| CN106146194B (en) | A kind of anti-continuous cropping microbial inoculum | |
| CN105670973A (en) | Bacillus methylotrophicus, bactericide and preparation method and application of bactericide | |
| CN106734184A (en) | A kind of in-situ remediation method of heavy-metal contaminated soil | |
| CN105198509A (en) | Special biological organic fertilizer for cotton and preparation method of special biological organic fertilizer | |
| CN103931664A (en) | Low-temperature resisting method for festuca arundinacea by using complex microbial inoculants | |
| Szczech et al. | The effect of plant growth promoting bacteria on transplants growth and lettuce yield in organic production | |
| CN109553478A (en) | A kind of biological organic fertilizer and preparation method thereof using the rape stalk exploitation dedicated biological and ecological methods to prevent plant disease, pests, and erosion function of rape | |
| Faravani et al. | Biological effects of fertilizer treatments on growth, yield and yield components of black cumin | |
| Zuraihah et al. | Effects of IMO and EM application on soil nutrients, microbial population and crop yield | |
| CN106831091A (en) | A kind of geobiont repair |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170613 |
|
| RJ01 | Rejection of invention patent application after publication |