CN106822597A - A kind of novel fermentation Chinese medicine and its production and use - Google Patents
A kind of novel fermentation Chinese medicine and its production and use Download PDFInfo
- Publication number
- CN106822597A CN106822597A CN201611221449.6A CN201611221449A CN106822597A CN 106822597 A CN106822597 A CN 106822597A CN 201611221449 A CN201611221449 A CN 201611221449A CN 106822597 A CN106822597 A CN 106822597A
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- fine powder
- fermentation
- radix astragali
- ganoderma lucidum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
- A61K36/424—Gynostemma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of novel fermentation Chinese medicine, belong to the field of Chinese medicines.The method that it passes through fermented tcm is obtained, and Chinese herb is：Dendrobium candidum, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, the Radix Astragali and gynostemma pentaphyllum, the mass ratio of wherein dendrobium candidum, the gold wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, the Radix Astragali and gynostemma pentaphyllum is (0.5~2.5)：(0.5~2.5)：(0.5~1.5)：(0.5~1.5)：(0.5~1.5)：1.Fermented tcm proposed by the present invention has highly enriched, high-purity, the characteristic of new active material, has preferable effect in terms of virus, cancer cell, and raising body immunity, removing oxyradical is killed, and it is expected with important medical value.
Description
Technical field
The present invention is Chinese medicine new technology, new technical field, more particularly to a kind of novel fermentation Chinese medicine and preparation method thereof and
Purposes.
Background technology
In Davos forums of Switzerland in 2016, Ma Yun states outright in face of world's media：Alibaba will put key target for investment in future
In environmental protection and health industry, any one responsible enterpriser should be the health environment effort of the mankind.Horse cloud what one says during a conversation,
Whole China Health risk anxious to be resolved is said.With China's economic sustainable growth, Chinese Hospitals are built more and more, and medical treatment sets
Standby increasingly advanced, medicine new results are come forth in large number, and new drug, specific drug constantly update.But, hypertension, heart more refractory in the past
Disease, diabetes ... cannot still be effected a radical cure；Originally various cancers rare to see as " infectious disease " constantly from aging to
Become younger and spread.
Chinese medicine is increasingly valued by people in recent years as traditional rarity of Chinese name.Fermented tcm is used as one
Kind new mode, be by natural drug (Chinese medicine) extract solution with preferred beneficial bacteria of intestinal tract flora one or more, one plant or
Several probiotics, using microecology, bionic method, by way of biological grafting, simulate people in vitro as strain
Digestion decomposable process of the intestinal environment and traditional Chinese medicine ingredients of body in human body, the Effective Component of Chinese Medicine to extracting carries out biology and turns
Change, by the macromolecular substances of Chinese medicine, the small molecule composition that can be directly absorbed by human body intestinal canal is turned into by microorganism conversion, make
Chinese medicine turns into the quick newtype drug for absorbing, quantifying curative effect.In fact, the Chinese medicine preparation in traditional Chinese medicine production is just utilized
Natural microbial herb fermenting is improving herbal medicine efficacy, eliminate herbal toxic effect, change herbal nature.For example：Medicated Leaven,
The traditional Chinese medicines such as Fermented Soybean, Rhizoma Pinelliae Fermentata are formed by natural microbial fermentation.However, traditional herb fermenting is using natural micro- life
Thing ferments, and strain is impure, and metabolism is theoretical, effective component theory is smudgy, produces poor controllability.Meanwhile, Partial fermentation Chinese medicine
Complicated component, production process is difficult to control.As Application No. 201610189936.2,201610092979.9,
201610189936.2 patent of invention discloses the probiotics fermention Chinese medicine composition for the treatment of cancer, Chinese medicine material therein
There are as many as tens of tastes, and fermentation process is relatively complicated.
The content of the invention
It is an object of the invention to solve problems of the prior art, and provide a kind of novel fermentation Chinese medicine.This hair
Bright used technical scheme is as follows：
Novel fermentation Chinese medicine of the invention is obtained by the method for fermented tcm, and its Chinese herb is：Iron sheet stone
Dry measure used in former times, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, the Radix Astragali and gynostemma pentaphyllum, wherein dendrobium candidum, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, Huang
The mass ratio of stilbene and gynostemma pentaphyllum is (0.5~2.5)：(0.5~2.5)：(0.5~1.5)：(0.5~1.5)：(0.5~1.5)：1.
Aforementioned proportion can further be preferably：The weight of dendrobium candidum, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, the Radix Astragali and gynostemma pentaphyllum
Than：2：2：1：1：1：1.Preferably using dendrobium candidum and HERBA DENDROBII, two kinds of stems of noble dendrobium have more preferable drug effect to the stem of noble dendrobium.
Preferably, described dendrobium candidum, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, the Radix Astragali and gynostemma pentaphyllum are concentration and carry
Take thing fine powder.Further, the concentration extraction ratio of the concentrated extract fine powder is 10~20：1.
Another object of the present invention is to provide a kind of preparation method of above-mentioned fermented tcm, its step is as follows：
Dendrobium candidum, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, the six kinds of Chinese medicine former materials of the Radix Astragali and gynostemma pentaphyllum that will be cleaned and crush
Material, prepares the concentrated extract fine powder of each raw material；Above-mentioned concentrated extract fine powder is mixed in proportion again, after adding water
Alternating temperature solid state fermentation is carried out, finished product will be obtained after zymotic fluid decompression, vacuum drying process.
Concentrated extract fine powder preparation method can also be prepared using the method for existing other Chinese medicine fine powders, but the present invention is provided
A kind of preferred preparation method, it is specific as follows：The Chinese herb that will be cleaned and crush, the pressurised extraction in water alcohol mixed liquor,
60~120 DEG C of Extracting temperature, while slow mechanical is stirred and controls to extract the reaction time for 8~12 hours；Extract solution is through over-subtraction
After pressing refined, spray drying, the concentrated extract fine powder of the Chinese herb is obtained respectively.
Preferably, during described temperature controlled fermentation, fermentation temperature is 10~75 DEG C, fermentation time is 48~72 small
When.
Fermented tcm of the invention can be used to prepare cancer therapy drug, especially for lung cancer or liver cancer with preferably effect
Really.
A few taste medicinal materials in the present invention are in itself selenium-rich medicinal materials, raw material first are made into extract fine powder through hyperconcetration, so
Afterwards by this hyperconcetration system or extract fine powder, then fermented tcm is obtained through solid-state alternating temperature control fermentation technique.It is this brand-new
Fermented tcm has numerous big advantages：
1st, have than traditional Chinese medicine drug effect and increase substantially, the activation of active ingredient small molecule state；
2nd, fermented tcm is inverted, and utilization, and almost non-toxic side effect can be preferably absorbed by organisms in a short time；
3rd, hyperconcetration and the high-purity of active principle are realized, by fermentation after medicinal material, have rich in high dose high activity
Machine selenium (some phenols ketone compounds of the Se-P II of selenium polysaccharide Se-P I and selenium) and other active materials；
The present invention is killing cancer cell (particularly lung cancer and liver cancer), virus, and raising body immunity, removing oxidation
Free radical aspect has preferable effect.Specific drug effect of the invention will be described in detail in subsequent embodiment.
Specific embodiment
The present invention is further elaborated with reference to embodiment, so that those skilled in the art more fully understand the present invention
Essence.Reagent, raw material and strain such as no special explanation employed in the present invention, using commercially available existing product.
Embodiment 1
In the present embodiment, prepared by the concentrated extract fine powder to five kinds of selenium-enriched Chinese medicines.Six kinds of Chinese medicine materials point
Wei not dendrobium candidum, HERBA DENDROBII, fructus schisandrae (i.e. the fruit of Chinese magnoliavine), ganoderma lucidum, the Radix Astragali, gynostemma pentaphyllum.In the present embodiment, raw material is from lake
Northern Enshi district buying, Chinese medicine material is rich in selenium Organic Selenium --- some phenols ketones of the Se-P II of selenium polysaccharide Se-P I and selenium
Compound (can also use other regional raw materials) certainly in preparation process.Above-mentioned raw material are cleaned and crush standby.
The six kinds of selenium-enriched Chinese herbs that will be cleaned and crush, in water alcohol mixed liquor (water：Ethanol=7：3, volume ratio) in
Extract, control 60~120 DEG C of temperature, meanwhile, slow mechanical is stirred and controls the reaction time：8~12 hours, protected in extraction process
Hold micro- pressurized state.After test of many times, final concentration extraction is 10~20 than scope：1.
After completing to extract, by extract solution decompression is refined, spray drying, obtain high content, five kinds of high-purity it is selenium-enriched in
The fine powder of medicine raw material, its active principle content improves 10~20 times.
It should be noted that in the present embodiment, its final purpose is to obtain the concentrated extract essence of selenium-enriched Chinese medicine
Powder.Above-mentioned preparation technology is each technological parameter in a preferred embodiment, but preparation process of the invention and the places of origin of raw materials, pre-
Processing method, apparatus etc. can be adjusted according to actual conditions.Through overtesting, technological parameter is adjusted within the above range
Whole, control temperature is higher in extraction process, and the reaction time can accordingly shorten, and will not produce considerable influence to final drug effect,
Substantially stabilization can be kept.
Embodiment 2
In the present embodiment, novel fermentation Chinese medicine is prepared using the concentrated extract fine powder obtained in embodiment 1.Its step is such as
Under：
A. by above-mentioned six kinds of obtained raw material dendrobium candidums, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, the Radix Astragali, gynostemma pentaphylla essence
Powder, by 2：2：1：1：1：Mineral water is added after 1 mass ratio mixing, alternating temperature solid-state is sent out during the mixture is placed on into fermentation tank
Ferment, while putting into bacillus polymyxa (Bacillus polymyxa) and lichens gemma in 0.15% ratio of mixture quality
The fermentation cenobium that bacillus (Bacilluslicheniformis) mixes (is commercially available prod, purchased from Shanxi Province Linfen Yao all
Area Fenhe River Amino Acid Factory；Also other commercially available prod can be used), control fermentation temperature by 10 DEG C gradually alternating temperature to 75 DEG C.This process
In, it is 60 hours that slow mechanical is stirred and controls the reaction time.
B. above-mentioned obtained zymotic fluid decompression, vacuum drying are obtained into Chinese medicine finished product.
Embodiment 3
In the present embodiment, novel fermentation Chinese medicine is prepared using the concentrated extract fine powder obtained in embodiment 1.Its step is such as
Under：
A. by above-mentioned six kinds of obtained raw material dendrobium candidums, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, the Radix Astragali, gynostemma pentaphylla essence
Powder, by 1：1：1：1：1：Mineral water is added after 1 mass ratio mixing, alternating temperature solid-state is sent out during the mixture is placed on into fermentation tank
Ferment, at the same in mixture quality 0.22% ratio put into equal proportion bacillus polymyxa (Bacillus polymyxa) and
(strain is commercially available prod to the fermentation cenobium that bacillus licheniformis (Bacilluslicheniformis) mixes, with embodiment
2 is identical), control fermentation temperature by 20 DEG C gradually alternating temperature to 75 DEG C.During this, slow mechanical is stirred and controls the reaction time to be
50 hours.
B. above-mentioned obtained zymotic fluid decompression, vacuum drying are obtained into Chinese medicine finished product.
Embodiment 4
In the present embodiment, novel fermentation Chinese medicine is prepared using the concentrated extract fine powder obtained in embodiment 1.Its step is such as
Under：
A. by above-mentioned six kinds of obtained dendrobium candidums, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, the Radix Astragali, gynostemma pentaphylla fine powder,
By 0.5：2.5：1.5：0.5：1.5：Mineral water is added after 1 mass ratio mixing, the alternating temperature solid state fermentation in fermentation tank, while pressing
0.15% ratio put into saccharomycete, control fermentation temperature by 15 DEG C gradually alternating temperature to 75 DEG C.During this, slow mechanical is stirred simultaneously
The 48 hours reaction time of control.
B. above-mentioned obtained zymotic fluid decompression, vacuum drying are obtained into finished product.
Embodiment 5
In the present embodiment, novel fermentation Chinese medicine is prepared using the concentrated extract fine powder obtained in embodiment 1.Its step is such as
Under：
A. by above-mentioned six kinds of obtained dendrobium candidums, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, the Radix Astragali, gynostemma pentaphylla fine powder,
By 2.5：0.5：0.5：1.5：0.5：Mineral water is added after 1 mass ratio mixing, the alternating temperature solid state fermentation in fermentation tank, while pressing
0.15% ratio put into lactic acid bacteria, control fermentation temperature by 10 DEG C gradually alternating temperature to 65 DEG C.During this, slow mechanical is stirred simultaneously
The 72 hours reaction time of control.
B. it is to obtain finished product by above-mentioned obtained zymotic fluid decompression, freeze-drying.
Embodiment 6
In the present embodiment, novel fermentation Chinese medicine is prepared using the concentrated extract fine powder obtained in embodiment 1.Its step is such as
Under：
A. by above-mentioned six kinds of obtained dendrobium candidums, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, the Radix Astragali, gynostemma pentaphylla fine powder,
By 1.75：2.0：0.9：1.15：0.85：Mineral water is added after 1 mass ratio mixing, the alternating temperature solid state fermentation in fermentation tank, together
When in 0.18% ratio put into Bifidobacterium, control fermentation temperature by 10 DEG C gradually alternating temperature to 65 DEG C.During this, slow mechanical
Stir and control the reaction time 72 hours.
B. it is to obtain Chinese medicine finished product by above-mentioned obtained zymotic fluid decompression, freeze-drying.
Embodiment 7
In the present embodiment, all steps and parameter are same as Example 6, and fermented bacterium is only changed to the how viscous bud of equal proportion
Spore bacillus (Bacillus polymyxa) and Bifidobacterium, final fermented liquid decompression, vacuum drying obtain Chinese medicine finished product.
Embodiment 8
In the present embodiment, all steps and parameter are same as Example 5, and fermented bacterium is only changed to the lichens bud of equal proportion
Spore bacillus (Bacilluslicheniformis) and lactic acid bacteria, final fermented liquid decompression, vacuum drying obtain Chinese medicine finished product.
Strain used is common food processing fungus strain row in embodiment 4~8, is common commercially available microbial inoculum, its denominator
It is engineering temperature controlling range between 10~40 DEG C.
Embodiment 9
In order to verify the effect of fermented tcm of the invention, its effect is surveyed by The National Center for Drug Screening
It is fixed, as a result show that the gained strain of above-described embodiment 2~9 is respectively provided with the inhibitory action to cancer cell, the best results of embodiment 2.
Below by taking the Chinese medicine of the gained of embodiment 2 as an example, provide corresponding test data and its effect is illustrated, remaining embodiment is no longer
Repeat, drug effect is substantially similar, only there is difference on growth of tumour cell inhibiting rate.
Anti-tumor biological body outer screening test 1
Sample number into spectrum：NC01090017 (fermented tcm that the embodiment 2 i.e. in the present invention is prepared)
Screening technique：Sulforhodamine B (sulforhodamine B, SRB)
Protein staining method
Cell line：A549 human lung cancers
Action time：72 hours
Result evaluation：It is inactive：1mg/ml<50%；
It is active：1mg/ml>50%；
To the inhibiting rate % of growth of tumour cell
Anti-tumor biological body outer screening test 2
Sample number into spectrum：NC01090017
Screening technique：Sulforhodamine B (sulforhodamine B, SRB)
Protein staining method
Cell line：BEL-7402 human lung cancers
Action time：72 hours
Result evaluation：It is inactive：1mg/ml<50%；
It is active：1mg/ml>50%；
To the inhibiting rate % of growth of tumour cell
Compound screens report to two plants of proliferation inhibition activities of human tumor cells
It is thin to two plants of human tumor cells human lung cancers that this experiment tests testing compound NC01090017 using SRB decoration methods
The inhibited proliferation of born of the same parents A-549 and human liver cancer cell BEL-7402, as a result shows, testing compound NC01090017 is to two plants
Human tumor cells have certain proliferation inhibition activity.
1.1 test objectives：Evaluate proliferation inhibition activities of the testing compound NC01090017 to human tumor cells, adriamycin
As positive control medicine.
1.2 sample sources and preparation：Testing compound NC01090017 normal salines face into 40mg/ml liquid storages
With preceding normal saline dilution to required concentration.
Reagent and instrument：
1) sulphonyl rhodamine B (sulforodamine B, SRB) is purchased from Sigma companies.
2) 96 porocyte culture plates are purchased from Corning companies.
3) key instrument：ELIASA (absorbance microplate reader molecular devices), purchase
From MD companies.
1.3 cell culture：Cell line used is as shown in table 1 in this experiment.All cells are placed in 37 DEG C containing 5%CO2It is full
With cellar culture in humidified incubator (Thermo Forma, the U.S.).
Organization type and source of the inhibited proliferation research using cell
1.4 test methods：Using Sulforhodamine B (sulforhodamine B, SRB) protein staining method.According to cell
Growth rate, 96 well culture plates are inoculated in by the tumour cell in exponential phase with 90 μ l/ holes, and adherent growth 24 hours is again
The μ l/ holes of dosing 10, each concentration sets three wells, and sets the physiological saline Vehicle controls and acellular zeroing hole of respective concentration.It is swollen
Oncocyte is cultivated 72 hours under the conditions of 37 DEG C, 5%CO2, and then incline nutrient solution, and cell is fixed with 10% cold TCA, and 4 DEG C put
With water washing is distilled 5 times after putting 1 hour, spontaneously dried in air.It is subsequently adding the SRB (Sigma) prepared by 1% glacial acetic acid
The μ L/ holes of 4mg/ml solution 100, dye 15 minutes in room temperature, remove supernatant, are washed with 1% acetic acid 5 times, are air-dried.Finally plus
Enter the Tris solution in 100 μ l/ holes, OD values are determined under ELIASA 560nm wavelength.Measured object is calculated by following equation to give birth to cancer cell
Inhibiting rate long, half amount of suppression IC50 values are calculated using Logit methods.Inhibiting rate=(control group OD values-administration group OD values)/
Control group OD value × 100%.
1.5 result of the tests：Compound N C01090017 has certain proliferation inhibition activity to two plants of tumour cells,
NC01090017 is 0.202mg/ml in the IC50 values of A-549, is 0.085mg/ml in the IC50 of BEL-7402.
1.6 experiment conclusions：Testing compound NC01090017 has certain Proliferation Ability to live to two plants of human tumor cells
Property.
Proliferation inhibition activity of the compound of table 1. to A-549 lung carcinoma cells
Proliferation inhibition activity of the compound of table 2. to BEL-7402 HCCs
Embodiment described above is a kind of preferably scheme of the invention, and so it is not intended to limiting the invention.Have
The those of ordinary skill for closing technical field, without departing from the spirit and scope of the present invention, can also make various changes
Change and modification.Therefore the technical scheme that all modes for taking equivalent or equivalent transformation are obtained, all falls within guarantor of the invention
In the range of shield.
Claims (9)
1. a kind of novel fermentation Chinese medicine, is obtained by the method for fermented tcm, it is characterised in that its Chinese herb is：Iron sheet
The stem of noble dendrobium, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, the Radix Astragali and gynostemma pentaphyllum, wherein dendrobium candidum, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum,
The mass ratio of the Radix Astragali and gynostemma pentaphyllum is（0.5~2.5）：（0.5~2.5）：（0.5~1.5）：（0.5~1.5）：（0.5~1.5）：1.
2. a kind of novel fermentation Chinese medicine according to claim 1, it is characterised in that dendrobium candidum, the golden wooden fork stem of noble dendrobium, Chinese Magnoliavine
The weight ratio of son, ganoderma lucidum, the Radix Astragali and gynostemma pentaphyllum：2：2：1：1：1：1.
3. a kind of novel fermentation Chinese medicine according to claim 1, it is characterised in that described dendrobium candidum, the golden wooden fork stem of noble dendrobium,
Fructus schisandrae, ganoderma lucidum, the Radix Astragali and gynostemma pentaphyllum are concentrated extract fine powder.
4. a kind of novel fermentation Chinese medicine according to claim 3, it is characterised in that the concentration of the concentrated extract fine powder
Extract than being 10~20：1.
5. a kind of preparation method of fermented tcm as claimed in claim 1, it is characterised in that step is as follows：
Dendrobium candidum, the golden wooden fork stem of noble dendrobium, fructus schisandrae, ganoderma lucidum, the six kinds of Chinese herbs of the Radix Astragali and gynostemma pentaphyllum, system that will be cleaned and crush
The standby concentrated extract fine powder for obtaining each raw material；Above-mentioned concentrated extract fine powder is mixed in proportion again, is carried out after adding water
Alternating temperature solid state fermentation, will obtain finished product after zymotic fluid decompression, vacuum drying process.
6. the preparation method of fermented tcm as claimed in claim 5, it is characterised in that the concentrated extract of each Chinese herb
Fine powder preparation method is as follows：The Chinese herb that will be cleaned and crush, the pressurised extraction in water alcohol mixed liquor, Extracting temperature 60~
120 DEG C, while slow mechanical is stirred and controls to extract the reaction time for 8~12 hours；Extract solution is refined by decompression, spraying is dry
After dry, the concentrated extract fine powder of the Chinese herb is obtained respectively.
7. the preparation method of fermented tcm as claimed in claim 5, it is characterised in that during described temperature controlled fermentation, hair
Ferment temperature is 10 ~ 75 DEG C, and fermentation time is 48~72 hours.
8. fermented tcm described in a kind of claim 1 is used to prepare the purposes of cancer therapy drug.
9. purposes as claimed in claim 8, it is characterised in that corresponding cancer is lung cancer or liver cancer.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610671653 | 2016-08-15 | ||
| CN2016106716531 | 2016-08-15 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106822597A true CN106822597A (en) | 2017-06-13 |
| CN106822597B CN106822597B (en) | 2020-12-25 |
Family
ID=59136340
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611221449.6A Active CN106822597B (en) | 2016-08-15 | 2016-12-26 | Fermented traditional Chinese medicine and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106822597B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109966427A (en) * | 2019-05-20 | 2019-07-05 | 北京震旦鼎泰健康管理有限公司 | A kind of fermentation living bacterial liquid and preparation method thereof treated tumour and improve immunity of organisms |
| CN110115378A (en) * | 2019-06-11 | 2019-08-13 | 福建省亚热带植物研究所 | A kind of compound dendrobium officinale ferment and preparation method thereof |
| CN114272327A (en) * | 2021-12-06 | 2022-04-05 | 芝绿大健康(广州)科技有限公司 | Dendrobium nobile fermented product and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105031301A (en) * | 2015-08-24 | 2015-11-11 | 周伟刚 | Anti-tumor traditional Chinese medicine composition and preparation method thereof |
| CN105707628A (en) * | 2016-03-11 | 2016-06-29 | 三株福尔制药有限公司 | Probiotic fermentation dendrobium officinale protocorm composition and preparation method thereof |
-
2016
- 2016-12-26 CN CN201611221449.6A patent/CN106822597B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105031301A (en) * | 2015-08-24 | 2015-11-11 | 周伟刚 | Anti-tumor traditional Chinese medicine composition and preparation method thereof |
| CN105707628A (en) * | 2016-03-11 | 2016-06-29 | 三株福尔制药有限公司 | Probiotic fermentation dendrobium officinale protocorm composition and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 孙晓生: "《孙晓生中医养生文丛（第一辑）》", 30 September 2014, 中国中医药出版社 * |
| 张朔生: "富硒虫草胶囊的毒理学实验研究", 《世界中西医结合杂志》 * |
| 赵中振等: "《当代药用植物典 第一册》", 30 September 2007, 上海世界图书出版社 * |
| 魏长志等: "《抗肿瘤中药集锦》", 31 August 2010, 中医古籍出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109966427A (en) * | 2019-05-20 | 2019-07-05 | 北京震旦鼎泰健康管理有限公司 | A kind of fermentation living bacterial liquid and preparation method thereof treated tumour and improve immunity of organisms |
| CN110115378A (en) * | 2019-06-11 | 2019-08-13 | 福建省亚热带植物研究所 | A kind of compound dendrobium officinale ferment and preparation method thereof |
| CN114272327A (en) * | 2021-12-06 | 2022-04-05 | 芝绿大健康(广州)科技有限公司 | Dendrobium nobile fermented product and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106822597B (en) | 2020-12-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104138527B (en) | A kind of biofermentation compositions and application thereof with antitumaous effect | |
| CN105039272B (en) | The preparation method of cactus fruits and vegetables enzyme and its cactus fruits and vegetables enzyme obtained | |
| WO2015143783A1 (en) | Natural conditioning and nourishing ferment and manufacturing method therefor | |
| KR101370386B1 (en) | Preparation method of fermented red ginseng using effective micro-organisms | |
| Xia et al. | Probiotic and related properties of a novel lactic acid bacteria strain isolated from fermented rose jam | |
| CN105941761B (en) | A kind of fermented by mixed bacterium tea bag and preparation method thereof | |
| CN102512458B (en) | Semi-bionic extraction method for active components of cordyceps militaris | |
| TW200819041A (en) | Method for cultivating Antrodia camphorate | |
| CN105861381A (en) | Method for lactobacillus plantarum fermented ginseng liquid | |
| WO2012097579A1 (en) | Pharmaceutical composition for treating melasma and preparation method therefor | |
| CN110934803B (en) | Plant fermentation composition with whitening and spot-fading functions | |
| CN113604395B (en) | Lactobacillus plantarum capable of fermenting dendrobium nobile and improving skin quality by fermentation liquor thereof | |
| KR20140063948A (en) | Manufacturing method of fermented red ginseng extract with enhanced ginsenosides rg3 and antioxidant activity | |
| CN106822597A (en) | A kind of novel fermentation Chinese medicine and its production and use | |
| CN106244487A (en) | One strain lactobacillus and improve the fermented ginseng method of rare saponin | |
| KR20100001087A (en) | A fermentation method of oriental herb remedy or grains | |
| CN106726949A (en) | A kind of grape pip proferment pulp cosmetic and preparation method and application | |
| CN104846017A (en) | Method for producing fleece-flower root red yeast rice | |
| CN110354207B (en) | Fermented traditional Chinese medicine oral liquid for improving gastrointestinal health of animals and preparation method thereof | |
| CN107668696A (en) | A kind of preparation method of ganoderma lucidum ferment | |
| KR101776613B1 (en) | Manufacturing Method for Biogenics using Vegetable Lactobacllus | |
| CN106119126A (en) | A kind of Moringa Ganoderma mycelium | |
| TWM590969U (en) | Particle structure of rutaceae plant fermentation broth with biomimetic stroma system | |
| CN113502230B (en) | Hericium erinaceus strain and culture method thereof, hericium erinaceus-ginseng bidirectional solid fermentation method and method for efficiently converting rare ginsenoside | |
| KR102350437B1 (en) | Cosmetic compositions containing Hwangryunhaedoktang fermented with Lactobacillus plantarum WiKim0111 for anti-acne |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |