CN110115378A - A kind of compound dendrobium officinale ferment and preparation method thereof - Google Patents
A kind of compound dendrobium officinale ferment and preparation method thereof Download PDFInfo
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- CN110115378A CN110115378A CN201910500353.0A CN201910500353A CN110115378A CN 110115378 A CN110115378 A CN 110115378A CN 201910500353 A CN201910500353 A CN 201910500353A CN 110115378 A CN110115378 A CN 110115378A
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- 150000001875 compounds Chemical class 0.000 title claims abstract description 41
- 241001076416 Dendrobium tosaense Species 0.000 title claims abstract description 37
- 238000000855 fermentation Methods 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000009636 Huang Qi Substances 0.000 claims abstract description 31
- 241000218231 Moraceae Species 0.000 claims abstract description 29
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 29
- 241000026010 Dendrobium candidum Species 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 230000004151 fermentation Effects 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 239000000463 material Substances 0.000 claims description 14
- 238000011081 inoculation Methods 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 8
- 239000008213 purified water Substances 0.000 claims description 8
- 238000011534 incubation Methods 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 241000582914 Saccharomyces uvarum Species 0.000 claims description 6
- 241000186605 Lactobacillus paracasei Species 0.000 claims description 4
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 2
- 239000003814 drug Substances 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 7
- 230000001580 bacterial effect Effects 0.000 abstract description 6
- 230000003345 hyperglycaemic effect Effects 0.000 abstract description 4
- 229930000044 secondary metabolite Natural products 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 244000005700 microbiome Species 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 235000013402 health food Nutrition 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 235000014101 wine Nutrition 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000721047 Danaus plexippus Species 0.000 description 1
- 240000004638 Dendrobium nobile Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000019991 rice wine Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- -1 small molecule compounds Chemical class 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000003462 zymogenic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of compound dendrobium officinale ferment and preparation method thereof, which is made of raw material from the following weight: dry product/fresh goods dendrobium candidum, dry product/fresh goods mulberries, 15-25 parts of rhizoma anemarrhenae, 10-20 parts of Radix Astragali, 10-20 parts of dark plum, 0.4-1.7 parts of zymophyte and 345-1550 parts of water；When the dendrobium candidum is dry product, parts by weight are 7.5-15 parts；When the dendrobium candidum is fresh goods, parts by weight are 15-30 parts；When the mulberries are dry product, parts by weight are 15-30 parts；When the mulberries are fresh goods, parts by weight are 30-60 parts；The rhizoma anemarrhenae, Radix Astragali and dark plum are dry product.The effect of compound dendrobium officinale ferment produced by the present invention not only has effects that Chinese medicine compound prescription collocation, enhances prescription auxiliary hyperglycemic is also fully utilized by the advantages of single bacterial strain is fermented, and can effectively ensure that the stability of fermentation secondary metabolite.
Description
Technical field
The present invention relates to processing of crude drugs technical fields, and in particular to a kind of compound dendrobium officinale ferment and preparation method thereof.
Background technique
Enzyme food technology can trace back to ancient brewing technology and China's fermentation sauce technology processed earliest.A long time ago,
South China, which just has, is made saccharomycete with local method rice, and rice wine is made, and is used to guests.Enzyme food commercially produces the beginning
In the Japan in last century.Initially because product price is high, consumption is limited.With the progress of technique, start extensive
Batch production.The scientist in Taiwan is scientific and technical using botany, cytology, nutrition, microbiology and modern biochemistry,
This traditional handicraft is improved, Taiwan abounds with the inherent advantage of tropical vegetables and fruits in addition, and enzyme food is made to be more in line with the modern times
Needs and balance of the people to nutrition.Since technique further improves, production cost further declines.Modern enzyme food has been dashed forward
Having broken that has the ancient technique of single culture and single culture medium, but uses controllable microbial fermentation technology,
With more than ten kinds to nearly hundred kinds vegetables and fruits, fermented using several probiotics.Ferment class health food be based on fresh fruit and
The natural edible raw materials such as vegetables, using the fermentation of microorganism, a large amount of metabolites generated and accumulation is required are final to obtain
Consumable products with special dietary and healthcare function.
Occurs the health food of some addition ferment on domestic market, but in other field of food using also very
It is few, still there is wide development space.In Japan and China Taiwan, ferment based food as a kind of functional food very
It is welcome.There are more than 40 years production histories in the manufacturer of Taiwan ferment class health food, there is over one hundred kind of product, has extensive
Popularity and public praise.Asia other areas such as South Korea, Malaysia, be also in great demand very much to Indonesia.In recent years,
In Europe and the U.S., Canada, ferment class health food is also gradually fashionable.
But due to the diversity of microbe species and its growing environment, cometabolism made from a variety of zymophyte co-fermentations
Product stability is poor, it is uncertain to easily lead to ferment product composition, it is more difficult to standardized production ferment product.
Summary of the invention
The present invention provides a kind of compound dendrobium officinale ferment and preparation method thereof, which not only has
The effect of Chinese medicine compound prescription is arranged in pairs or groups, the effect of enhancing prescription auxiliary hyperglycemic, the advantages of being also fully utilized by single bacterial strain fermentation,
It can effectively ensure that the stability of fermentation secondary metabolite.
The present invention adopts the following technical scheme:
A kind of compound dendrobium officinale ferment, is made of raw material from the following weight:
Dry product/fresh goods dendrobium candidum, dry product/fresh goods mulberries, 15-25 parts of rhizoma anemarrhenae, 10-20 parts of Radix Astragali, dark plum 10-20
Part, 0.4-1.7 parts of zymophyte and 345-1550 parts of water；
When the dendrobium candidum is dry product, parts by weight are 7.5-15 parts；It is heavy when the dendrobium candidum is fresh goods
Measuring part is 15-30 parts；
When the mulberries are dry product, parts by weight are 15-30 parts；When the mulberries are fresh goods, parts by weight 30-
60 parts；
The rhizoma anemarrhenae, Radix Astragali and dark plum are dry product.
Preferably, a kind of compound dendrobium officinale ferment, is made of raw material from the following weight: the dendrobium candidum 25 of fresh goods
Part, 15 parts of 15 parts of 20 parts of 25 parts of mulberries, rhizoma anemarrhenae, Radix Astragali, the dark plum of dry product, 1.1 parts of zymophyte and 1000 parts of water.
Preferably, the zymophyte be lactobacillus paracasei, Lactobacillus rhamnosus, streptococcus thermophilus, saccharomyces uvarum or
Any one in saccharomyces cerevisiae.
Preferably, the water is purified water.
A kind of preparation method of compound dendrobium officinale ferment, sequentially includes the following steps:
Dry product/fresh goods dendrobium candidum, dry product/fresh goods mulberries, rhizoma anemarrhenae, Radix Astragali and dark plum are weighed, the above medicinal material is done
Product are ground into coarse powder and medicinal material fresh goods are cut into segment, add 6-10 times measure purified water be uniformly mixed；Then it is in temperature
Sterilize 15-30min under conditions of 100-130 DEG C, is let cool after sterilizing to room temperature；Finally according to 1 ‰ ratio inoculation fermentation bacterium, inoculation
Constant temperature incubation 72-96h under the conditions of mixed liquor is put into 26-30 DEG C afterwards, then through being centrifuged, compound iron sheet is made in separate ferment solution
Dendrobium nobile ferment.
Preferably, the microbe colony of the compound dendrobium officinale ferment obtained counts >=109。
After adopting the above technical scheme, the present invention has the advantages that compared with existing background technique
Compound dendrobium officinale ferment produced by the present invention take full advantage of Chinese medicine compound prescription collocation the effect of, by dendrobium candidum,
The medicinal materials such as mulberries, rhizoma anemarrhenae, Radix Astragali and dark plum are reasonably combined, and pass through zymogenic fermentation, enhance compound dendrobium officinale
The effect of ferment auxiliary hyperglycemic.
Compound dendrobium officinale ferment produced by the present invention not only makes full use of Chinese medicine the monarch and his subjects to help the collocation of generation, utilizes single bacterium
The effect of stability of fermentation secondary metabolite is effectively ensured in strain fermentation, enhances prescription auxiliary hyperglycemic, also has ferment
Food small molecule, easy the advantages of absorbing.
Any foreign aid's nutrition is not added for compound dendrobium officinale ferment produced by the present invention, all using drug carbon source itself,
The nutritional ingredients such as nitrogen source, inorganic salts sufficiently can convert the ammonia that human body more easily absorbs for macromolecular organic principle in Chinese medicine
The small molecule compounds such as base acid, glucose.
Compound dendrobium officinale ferment produced by the present invention is studied through non-targeted dynamic metabolism, fermentation front and back product contrast table
Bright: 97 kinds of metabolites such as arginine, sucrose, aspartic acid, serine, adenosine, gland propylhomoserin, choline glycerophosphatide have significant
Difference.
Specific embodiment
Some embodiments of the present invention presented below, to help the present invention, but protection scope of the present invention is further understood
It is not limited to these embodiments.
Embodiment one
A kind of compound dendrobium officinale ferment, is made of raw material from the following weight:
25 parts of dendrobium candidum, 15 parts of 15 parts of 20 parts of 25 parts of mulberries, rhizoma anemarrhenae, Radix Astragali, the dark plum of dry product, zymophyte of fresh goods
1.1 parts and 1000 parts of water；The rhizoma anemarrhenae, Radix Astragali and dark plum are dry product.
A kind of preparation method of above-mentioned compound dendrobium officinale ferment, sequentially includes the following steps:
Weigh dendrobium candidum, mulberries, rhizoma anemarrhenae, Radix Astragali and dark plum respectively, after by mulberries, rhizoma anemarrhenae, Radix Astragali and dark plum dry product medicine
Material is ground into coarse powder, and iron-sheet dendrobe fresh product medicinal material is cut into segment, adds 1000 parts of purified water and is uniformly mixed；Then in temperature
Sterilize 20min under conditions of being 121 DEG C, is let cool after sterilizing to room temperature；Finally it is inoculated with 1.1 parts of saccharomyces uvarum bacterial strain, after inoculation
Constant temperature incubation 96h under the conditions of mixed liquor is put into 26 DEG C, then through being centrifuged, compound dendrobium officinale ferment is made in separate ferment solution；
The saccharomyces uvarum bacterial strain is provided by Chinese microorganism strain collection.
Embodiment two
A kind of compound dendrobium officinale ferment, is made of raw material from the following weight:
15 parts of dendrobium candidum, 15 parts of 15 parts of 25 parts of 30 parts of mulberries, rhizoma anemarrhenae, Radix Astragali, the dark plum of dry product, zymophyte of dry product
0.9 part and 800 parts of water；The rhizoma anemarrhenae, Radix Astragali and dark plum are dry product.
A kind of preparation method of above-mentioned compound dendrobium officinale ferment, sequentially includes the following steps:
Weigh dendrobium candidum, mulberries, rhizoma anemarrhenae, Radix Astragali and dark plum respectively, after by dendrobium candidum, mulberries, rhizoma anemarrhenae, Radix Astragali and crow
Dried plum product pulverizing medicinal materials add 800 parts of purified water and are uniformly mixed at coarse powder；Then it sterilizes under conditions of temperature is 121 DEG C
20min is let cool after sterilizing to room temperature；It is finally inoculated with 0.9 part of Wine brewing yeast strain, mixed liquor is put under the conditions of 28 DEG C after inoculation
Constant temperature incubation 96h, then through being centrifuged, compound dendrobium officinale ferment is made in separate ferment solution；The Wine brewing yeast strain is by China
Culture Collection provides.
Embodiment three
A kind of compound dendrobium officinale ferment, is made of raw material from the following weight:
25 parts of dendrobium candidum, 15 parts of 15 parts of 20 parts of 25 parts of mulberries, rhizoma anemarrhenae, Radix Astragali, the dark plum of dry product, zymophyte of fresh goods
1.1 parts and 1000 parts of water；The rhizoma anemarrhenae, Radix Astragali and dark plum are dry product.
A kind of preparation method of above-mentioned compound dendrobium officinale ferment, sequentially includes the following steps:
Weigh dendrobium candidum, mulberries, rhizoma anemarrhenae, Radix Astragali and dark plum respectively, after by mulberries, rhizoma anemarrhenae, Radix Astragali and dark plum dry product medicine
Material is ground into coarse powder, and iron-sheet dendrobe fresh product medicinal material is cut into segment, adds 1000 parts of purified water and is uniformly mixed；Then in temperature
Sterilize 20min under conditions of being 121 DEG C, is let cool after sterilizing to room temperature；1.1 parts of strains of lactobacillus paracasei are finally inoculated with, inoculation
Constant temperature incubation 72h under the conditions of mixed liquor is put into 28 DEG C afterwards, then through being centrifuged, compound dendrobium officinale ferment is made in separate ferment solution
Element；The strains of lactobacillus paracasei is provided by Chinese microorganism strain collection.
Example IV
A kind of compound dendrobium officinale ferment, is made of raw material from the following weight:
25 parts of dendrobium candidum, 15 parts of 15 parts of 15 parts of 30 parts of mulberries, rhizoma anemarrhenae, Radix Astragali, the dark plum of fresh goods, zymophyte of fresh goods
0.7 part and 600 parts of water；The rhizoma anemarrhenae, Radix Astragali and dark plum are dry product.
A kind of preparation method of above-mentioned compound dendrobium officinale ferment, sequentially includes the following steps:
Weigh dendrobium candidum, mulberries, rhizoma anemarrhenae, Radix Astragali and dark plum respectively, by rhizoma anemarrhenae, Radix Astragali and dark plum dry product pulverizing medicinal materials at
Dendrobium candidum and mulberries fresh goods medicinal material are cut into segment by coarse powder, are added 600 parts of purified water and are uniformly mixed；Then it is in temperature
Sterilize 20min under conditions of 121 DEG C, is let cool after sterilizing to room temperature；It is finally inoculated with 0.7 part of saccharomyces uvarum bacterial strain, it will after inoculation
Constant temperature incubation 96h under the conditions of mixed liquor is put into 26 DEG C, then through being centrifuged, compound dendrobium officinale ferment is made in separate ferment solution；Institute
Saccharomyces uvarum bacterial strain is stated to be provided by Chinese microorganism strain collection.
Embodiment five
A kind of compound dendrobium officinale ferment, is made of raw material from the following weight:
20 parts of dendrobium candidum, 20 parts of 20 parts of 20 parts of 20 parts of mulberries, rhizoma anemarrhenae, Radix Astragali, the dark plum of fresh goods, zymophyte of fresh goods
0.9 part and 800 parts of water；The rhizoma anemarrhenae, Radix Astragali and dark plum are dry product.
A kind of preparation method of above-mentioned compound dendrobium officinale ferment, sequentially includes the following steps:
Weigh dendrobium candidum, mulberries, rhizoma anemarrhenae, Radix Astragali and dark plum respectively, by rhizoma anemarrhenae, Radix Astragali and dark plum dry product pulverizing medicinal materials at
Dendrobium candidum and mulberries fresh goods medicinal material are cut into segment by coarse powder, are added 800 parts of purified water and are uniformly mixed；Then it is in temperature
Sterilize 20min under conditions of 121 DEG C, is let cool after sterilizing to room temperature；Finally it is inoculated with 0.9 part of Lactobacillus rhamnosus strain, after inoculation
Constant temperature incubation 72h under the conditions of mixed liquor is put into 30 DEG C, then through being centrifuged, compound dendrobium officinale ferment is made in separate ferment solution；
The Lactobacillus rhamnosus strain is provided by Chinese microorganism strain collection.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (6)
1. a kind of compound dendrobium officinale ferment, which is characterized in that be made of raw material from the following weight:
Dry product/fresh goods dendrobium candidum, dry product/fresh goods mulberries, 15-25 parts of rhizoma anemarrhenae, 10-20 parts of Radix Astragali, 10-20 parts of dark plum,
0.4-1.7 parts and water 345-1550 parts of zymophyte；
When the dendrobium candidum is dry product, parts by weight are 7.5-15 parts；When the dendrobium candidum is fresh goods, parts by weight
It is 15-30 parts；
When the mulberries are dry product, parts by weight are 15-30 parts；When the mulberries are fresh goods, parts by weight 30-60
Part；
The rhizoma anemarrhenae, Radix Astragali and dark plum are dry product.
2. a kind of compound dendrobium officinale ferment as described in claim 1, which is characterized in that by the raw material system of following parts by weight
At 25 parts of the dendrobium candidum of: fresh goods, 15 parts of 15 parts of 20 parts of 25 parts of mulberries, rhizoma anemarrhenae, Radix Astragali, the dark plum of dry product, 1.1 parts of zymophyte and
1000 parts of water.
3. such as a kind of described in any item compound dendrobium officinale ferment of claims 1 or 2, it is characterised in that: the zymophyte is
Any one in lactobacillus paracasei, Lactobacillus rhamnosus, streptococcus thermophilus, saccharomyces uvarum or saccharomyces cerevisiae.
4. such as a kind of described in any item compound dendrobium officinale ferment of claims 1 or 2, it is characterised in that: the water is purifying
Water.
5. a kind of preparation method of compound dendrobium officinale ferment according to any one of claims 1-4, which is characterized in that by with
Lower step carries out:
Dry product/fresh goods dendrobium candidum, dry product/fresh goods mulberries, rhizoma anemarrhenae, Radix Astragali and dark plum are weighed, by the above medicinal material dry product powder
Be broken into coarse powder and medicinal material fresh goods be cut into segment, add 6-10 times measure purified water be uniformly mixed；It then is 100- in temperature
Sterilize 15-30min under conditions of 130 DEG C, is let cool after sterilizing to room temperature；It, will after inoculation finally according to 1 ‰ ratio inoculation fermentation bacterium
Constant temperature incubation 72-96h under the conditions of mixed liquor is put into 26-30 DEG C, then through being centrifuged, compound dendrobium officinale is made in separate ferment solution
Ferment.
6. a kind of preparation method of compound dendrobium officinale ferment as claimed in claim 5, it is characterised in that: obtained described multiple
The microbe colony of square dendrobium candidum ferment counts >=109。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112385834A (en) * | 2020-11-16 | 2021-02-23 | 九仙尊霍山石斛股份有限公司 | Preparation method and application of dendrobium huoshanense enzyme with anti-saccharification and metabolism-improving functions |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105687771A (en) * | 2016-02-18 | 2016-06-22 | 云南久丽康源石斛开发有限公司 | Herba dendrobii capsule with auxiliary blood glucose lowering function and method for preparing herba dendrobii capsule |
| CN106722928A (en) * | 2016-11-14 | 2017-05-31 | 霍山三宝生物保健品有限公司 | A kind of stem of noble dendrobium ferment production method with along intestines bowel relaxing functions |
| CN106822597A (en) * | 2016-08-15 | 2017-06-13 | 冯惠忠 | A kind of novel fermentation Chinese medicine and its production and use |
| CN109730311A (en) * | 2019-02-01 | 2019-05-10 | 安徽大学 | A kind of hoveniae semoveniae semen composite enzyme, preparation method and application |
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| CN105687771A (en) * | 2016-02-18 | 2016-06-22 | 云南久丽康源石斛开发有限公司 | Herba dendrobii capsule with auxiliary blood glucose lowering function and method for preparing herba dendrobii capsule |
| CN106822597A (en) * | 2016-08-15 | 2017-06-13 | 冯惠忠 | A kind of novel fermentation Chinese medicine and its production and use |
| CN106722928A (en) * | 2016-11-14 | 2017-05-31 | 霍山三宝生物保健品有限公司 | A kind of stem of noble dendrobium ferment production method with along intestines bowel relaxing functions |
| CN109730311A (en) * | 2019-02-01 | 2019-05-10 | 安徽大学 | A kind of hoveniae semoveniae semen composite enzyme, preparation method and application |
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| CN112385834A (en) * | 2020-11-16 | 2021-02-23 | 九仙尊霍山石斛股份有限公司 | Preparation method and application of dendrobium huoshanense enzyme with anti-saccharification and metabolism-improving functions |
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