CN106818639A - A kind of method for improving the maternal sheep output of milk - Google Patents
A kind of method for improving the maternal sheep output of milk Download PDFInfo
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- 241001494479 Pecora Species 0.000 title claims abstract description 92
- 239000008267 milk Substances 0.000 title claims abstract description 31
- 210000004080 milk Anatomy 0.000 title claims abstract description 31
- 235000013336 milk Nutrition 0.000 title claims abstract description 31
- 230000008774 maternal effect Effects 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 15
- 235000019687 Lamb Nutrition 0.000 claims abstract description 13
- 210000000481 breast Anatomy 0.000 claims abstract description 11
- 238000009396 hybridization Methods 0.000 claims abstract description 9
- 230000037396 body weight Effects 0.000 claims abstract description 6
- 108020004999 messenger RNA Proteins 0.000 claims abstract description 6
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 6
- 238000010171 animal model Methods 0.000 claims abstract description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 72
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims description 54
- 235000019441 ethanol Nutrition 0.000 claims description 43
- 239000007788 liquid Substances 0.000 claims description 33
- 239000012188 paraffin wax Substances 0.000 claims description 15
- 238000004043 dyeing Methods 0.000 claims description 12
- 210000004369 blood Anatomy 0.000 claims description 11
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 238000004458 analytical method Methods 0.000 claims description 6
- 239000008280 blood Substances 0.000 claims description 5
- 235000002687 Caesalpinia echinata Nutrition 0.000 claims description 3
- 241000127464 Paubrasilia echinata Species 0.000 claims description 3
- 241000282887 Suidae Species 0.000 claims description 3
- 229960000583 acetic acid Drugs 0.000 claims description 3
- 230000004069 differentiation Effects 0.000 claims description 3
- 238000004821 distillation Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 claims description 3
- 239000012362 glacial acetic acid Substances 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 3
- 230000009027 insemination Effects 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- 229920001206 natural gum Polymers 0.000 claims description 3
- 230000000474 nursing effect Effects 0.000 claims description 3
- 238000005464 sample preparation method Methods 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims description 3
- 239000004575 stone Substances 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 239000001993 wax Substances 0.000 claims description 3
- 229930040373 Paraformaldehyde Natural products 0.000 claims description 2
- 229920002866 paraformaldehyde Polymers 0.000 claims description 2
- 238000002386 leaching Methods 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 7
- 230000006651 lactation Effects 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 241000283903 Ovis aries Species 0.000 abstract description 3
- 230000007812 deficiency Effects 0.000 abstract description 2
- 238000010998 test method Methods 0.000 abstract description 2
- 241001465754 Metazoa Species 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 102100020948 Growth hormone receptor Human genes 0.000 description 2
- 108010031794 IGF Type 1 Receptor Proteins 0.000 description 2
- 102100039688 Insulin-like growth factor 1 receptor Human genes 0.000 description 2
- 108010068542 Somatotropin Receptors Proteins 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- 102000003998 progesterone receptors Human genes 0.000 description 2
- 108090000468 progesterone receptors Proteins 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 101001123448 Homo sapiens Prolactin receptor Proteins 0.000 description 1
- 102100024819 Prolactin Human genes 0.000 description 1
- 108010057464 Prolactin Proteins 0.000 description 1
- 102100029000 Prolactin receptor Human genes 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000007937 eating Effects 0.000 description 1
- 235000005686 eating Nutrition 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229940097325 prolactin Drugs 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
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- Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kind of method for improving the maternal sheep output of milk, the method is comprised the following steps：The selection of experimental animal, hybridization, body weight determination, output of milk measure, the mrna expression amount of breast tissue acceptor gene are determined.Test method of the invention passes through the gloomy rams of Dong Fuli and sheep ewe hybridization production output of milk East Lake F1 high, as three way cross female parent sheep, fundamentally the problems such as the maternal sheep lactation amount deficiency of solution, slow-growing lamb and mutton sheep culture benefit not high.Result is proved：First generation of hybrid sheep ewe is preferable female parent sheep, after Du Boyang hybridization, can feed 3 lambs, and culture benefit improves more than 30%.
Description
Technical field
The invention belongs to technical field of livestock husbandry, specifically, it is related to a kind of method for improving the maternal sheep output of milk.
Background technology
Sheep is the animal tamed earliest in Southwest Asia before about 11000, is mainly used in carnivorous source.Present generation
There is raising boundary various regions.China is the most country of the sheep number of animals raised in the world, wherein with Inner Mongol and the Northwest's number of animals raised most
Greatly, the national number of animals raised reaches 1.6 hundred million.Sheep disposition is docile, manageability.Sheep delicious meat, various nationalities' all eatings.It is most of
Sheep variety is long-term heat, and some local varieties are then season heat, more heat, breeding, spring lambing in the fall.Ewe
Period of pregnancy is 145~152 days, and per tire lambing 1~5, the survival rate of lamb depends on the output of milk of ewe, but China is most of
The sheep variety output of milk is not high, is typically only capable to support 1~2 lamb.Ewe birth weight in itself, weaning weight, breast volume and
Trophic level during shape, lambing month, litter size, lactation, the nutrition condition before lambing etc. all have one to be fixed to the output of milk
Ring.
Sheep has that reproductive capacity is high, adaptability is good, maternal instinct is strong, manageability the features such as, it is deep by the joyous of vast plant (family)
Meet, be widely used as mutton sheep hybridization maternal.The average lambing percentage of the kind is up to 270%~280%, and nest produces 3~4 mothers of lamb
More typically, but its output of milk generally only enough feeds 2 lambs to sheep, and polyembryony lamb can be died of hunger, survival rate less than 70%, serious shadow
Mutton sheep culture benefit is rung.Therefore, it is badly in need of a kind of method for improving the maternal sheep output of milk of exploitation.
The content of the invention
It is an object of the invention to provide a kind of method for improving the maternal sheep output of milk, the present invention is by importing Dong Fuli
Gloomy blood, is hybridized with the gloomy rams of Dong Fuli and sheep ewe, and East Lake F1 of production has the output of milk higher, can be used as ternary miscellaneous
Maternal sheep is handed over, fundamentally the problems such as the maternal sheep lactation amount deficiency of solution, slow-growing lamb and mutton sheep culture benefit not high；
The method improves the survival rate and mutton sheep benefit of lamb by solving the problems, such as the amount of sucking the breast of brood polyembryony lamb.
Its concrete technical scheme is：
A kind of method for improving the maternal sheep output of milk, comprises the following steps：
The selection of step 1, experimental animal
(1) parent sire of hybrid pigs sheep is the gloomy milk sheep of Dong Fuli, purchased from Australia；
(2) it is local sheep to hybridize maternal；
Step 2, hybridization
East Lake generation, i.e. F1, are after the gloomy rams of purebred Dong Fuli hybridize with purebred sheep containing 1/2 Dong Fulisen sheep bloods
Generation；It it is East Lake generation ram and purebred sheep ewe post-coitum generation containing 1/4 Dong Fulisen sheep bloods sheep；All ewes utilize
Technology of artificial insemination is bred；
Step 3, body weight determination
Sheep, East Lake generation sheep to the birth of the identical feeding and management condition lower same period, containing 1/4 Dong Fulisen sheep blood sheep
Birth weight, 2 monthly age weights, 4 monthly ages are measured, record again；
Step 4, the output of milk are determined
Identical feeding and management condition lower same period lambing ewe 3 is selected respectively, and the output of milk is entered with lamb weight weight method
Row is determined, record；
Step 5, the mrna expression amount of breast tissue acceptor gene are determined
5.1 samplings
Nursing period East Lake generation sheep test group and sheep control group breast tissue sample are gathered with modus operandi, paraformaldehyde is placed in
In take back laboratory, after fixed 48h, repair sample to 1cm3；
5.2 sample preparations
5.2.1 be dehydrated by the tissue sample after fixation be sequentially placed into 70% alcohol 30min, 80% alcohol 1h, 90% alcohol 1h,
100% alcohol 1h, changes an alcohol after 1h；
5.2.2 it is transparent with etc. than row prepare absolute ethyl alcohol, dimethylbenzene mixed solution soak 2h, then with dimethylbenzene I immersion
10min, changes dimethylbenzene, then steep 5min；
5.2.3 waxdip soaks 2h in dimethylbenzene, the mixed liquor of paraffin liquid prepared than row is waited, and 2h, paraffin are soaked in paraffin I liquid
2h is soaked in II liquid, drying in 60 DEG C of baking boxs is subsequently placed in；
5.2.4 embed and preheating embedding medium is poured into Simple carton, then the tissue sample after waxdip is placed on and is stood at room temperature；
5.2.5 cut into slices and embedded wax stone is placed on paraffin slicing machine, be cut into 5um thin slices, then extend piece, bonding die,
After mark, it is placed in slide holding frame, is dried under the conditions of 37 DEG C；
5.3 dyeing
(1) paraffin section is placed in dimethylbenzene I liquid 10min, dimethylbenzene II liquid 5min, 100% second by HE dyeing dewaxing successively
Alcohol I liquid 3min, 100% ethanol II liquid 3min, 90% ethanol 3min, 80% ethanol 3min, 70% ethanol 3min；
(2) dyeing brazilwood extract dyeing 2min, washing removes dye liquor, plus 5% glacial acetic acid differentiation 30s, and 5min is washed with distillation,
Eosin stains 1min is used again, is then examined under a microscope；
(3) decolourize for slide to be sequentially placed into 70% alcohol, 80% alcohol, 90% alcohol, 100% alcohol I liquid, 100% wine
Decolourize each 1min in smart II liquid, then respectively in dimethylbenzene I liquid and dimethylbenzene II liquid decolourings 5min；
(4) mounting takes out section, dries dimethylbenzene, and natural gum in drop, covered is dried at ambient temperature；
(5) take a picture：After being taken a picture with microscopic camera, graphical analysis is carried out；
5.4 data statistics and analysis
Dyeed by HE and East Lake generation sheep and sheep mammary structure are compared.
Compared with prior art, beneficial effects of the present invention：
Test method of the invention hybridizes production output of milk East Lake F1 high by the gloomy rams of Dong Fuli and sheep ewe, is used as
Three way cross female parent sheep, fundamentally the maternal sheep lactation amount of solution is not enough, lamb is slow-growing and mutton sheep culture benefit is not high
The problems such as.Result is proved：First generation of hybrid sheep ewe is comparatively ideal maternal sheep, after Du Boyang hybridization, can feed 3 lambs,
Culture benefit improves more than 30%.
Brief description of the drawings
Fig. 1 is different cultivars Pregnant Sheep breast tissue acceptor gene mrna expression amount.
Specific embodiment
Technical scheme is described in more detail with specific embodiment below in conjunction with the accompanying drawings.
First, body weight and the output of milk are determined
1 materials and methods
The selection of 1.1 experimental animals
(1) the gloomy milk sheep of parent sire of hybrid pigs sheep Dong Fuli is purchased from Australia.
(2) it is the local adult sheep raised to hybridize maternal sheep.
1.2 hybridization East Lake generation, i.e. F1, are the gloomy rams of purebred Dong Fuli and purebred sheep containing 1/2 Dong Fulisen sheep bloods
Filial generation；It it is East Lake generation ram and purebred sheep ewe post-coitum generation containing 1/4 Dong Fulisen sheep bloods sheep.All ewes
Bred using technology of artificial insemination.
Sheep that 1.3 body weight determination conventional methods are born to the identical feeding and management condition lower same period, East Lake generation sheep, contain
The birth weight of 1/4 Dong Fulisen sheep blood sheep, 2 monthly age weights, 4 monthly ages are measured, record again.
1.4 outputs of milk are determined and select identical feeding and management condition lower same period lambing ewe 3 respectively, are weighed with lamb weight
Method is measured to the output of milk, records.
2nd, the mrna expression amount of breast tissue acceptor gene is determined
1 sampling
Nursing period East Lake generation sheep (test group) and sheep (control group) breast tissue sample is gathered with modus operandi, poly is placed in
Laboratory is taken back in formaldehyde, after fixed 48h, sample to 1cm is repaiied3.
2 sample preparations
2.1 dehydration by the tissue sample after fixation be sequentially placed into 70% alcohol 30min, 80% alcohol 1h, 90% alcohol 1h,
100% alcohol 1h (changes an alcohol) after 1h.
2.2 it is transparent with etc. than row prepare absolute ethyl alcohol, dimethylbenzene mixed solution soak 2h, then with dimethylbenzene I immersion
10min, changes dimethylbenzene, then steep 5min.
2.3 waxdips soak 2h in dimethylbenzene, the mixed liquor of paraffin liquid prepared than row is waited, and 2h, paraffin II are soaked in paraffin I liquid
2h is soaked in liquid, drying in 60 DEG C of baking boxs is subsequently placed in.
Preheating embedding medium is poured into Simple carton by 2.4 embeddings, is then placed on the tissue sample after waxdip and is stood at room temperature.
Is placed in embedded wax stone on paraffin slicing machine by 2.5 sections, is cut into 5um thin slices, then extend piece, bonding die, mark
After note, it is placed in slide holding frame, is dried under the conditions of 37 DEG C.
3 dyeing
(1) paraffin section is placed in dimethylbenzene I liquid 10min, dimethylbenzene II liquid 5min, 100% second by HE dyeing dewaxing successively
Alcohol I liquid 3min, 100% ethanol II liquid 3min, 90% ethanol 3min, 80% ethanol 3min, 70% ethanol 3min.
(2) dyeing brazilwood extract dyeing 2min, washing removes dye liquor, plus 5% glacial acetic acid differentiation 30s, and 5min is washed with distillation,
Eosin stains 1min is used again, is then examined under a microscope.
(3) decolourize for slide to be sequentially placed into 70% alcohol, 80% alcohol, 90% alcohol, 100% alcohol I liquid, 100% wine
Decolourize each 1min in smart II liquid, then respectively in dimethylbenzene I liquid and dimethylbenzene II liquid decolourings 5min.
(4) mounting takes out section, dries dimethylbenzene, and natural gum in drop, covered is dried at ambient temperature.
(5) take a picture：After being taken a picture with microscopic camera, graphical analysis is carried out.
4 data statistics and analysis
Dyeed by HE and East Lake generation sheep and sheep mammary structure are compared.
3rd, result of the test
Sheep ewe and the gloomy ram hybridization of Dong Fuli, its first generation of hybrid sheep (abbreviation East Lake F1) birth weight, 2 monthly age weaning weights
With 4 monthly age body weight respectively than sheep 0.25kg, 3.74kg and the 3.65kg high under identical feeding and management condition, and appearance traits
More they tend to meat type.Average day milk amount reaches 2.36kg in East Lake F1 50 days ewe postpartum of adult, than identical feeding and management condition
Under sheep 0.46k kg high.But the output of milk of Hybrids sheep can decline (be shown in Table 1) with Dong Fuli is gloomy containing the decline of blood volume.
East Lake F1 speeds of growth of table 1 and the output of milk (kg)
* triplet birth 1~50 day twin lamb(s) ewe postpartum average milk production is referred to.
East Lake F1 pregnancy ewes breast tissues GHR (growth hormone receptor), PR (progesterone receptor), PRLR (receive by prolactin
Body), the mrna expression amount of IGF-IR (insulin-like growth factor I receptor) gene be all remarkably higher than sheep (see Fig. 1), this shows
F1 ewes have milk performance higher in East Lake.
The above, preferably specific embodiment only of the invention, protection scope of the present invention not limited to this are any ripe
Those skilled in the art are known in the technical scope of present disclosure, the letter of the technical scheme that can be become apparent to
Altered or equivalence replacement are each fallen within protection scope of the present invention.
Claims (1)
1. a kind of method for improving the maternal sheep output of milk, it is characterised in that comprise the following steps：
The selection of step 1, experimental animal
(1) parent sire of hybrid pigs sheep is the gloomy milk sheep of Dong Fuli；
(2) it is adult sheep to hybridize maternal sheep；
Step 2, hybridization
East Lake generation, i.e. F1, are the gloomy rams of purebred Dong Fuli and purebred sheep filial generation containing 1/2 Dong Fulisen sheep bloods；Contain
1/4 Dong Fulisen sheep bloods sheep is East Lake generation ram and purebred sheep ewe post-coitum generation；All ewes are using artificial
Insemination techniques are bred；
Step 3, body weight determination
Sheep, East Lake generation sheep to the birth of the identical feeding and management condition lower same period, containing 1/4 Dong Fulisen sheep blood sheep just
Raw weight, 2 monthly age weights, 4 monthly ages are measured, record again；
Step 4, the output of milk are determined
Identical feeding and management condition lower same period lambing ewe 3 is selected respectively, and the output of milk is surveyed with lamb weight weight method
Fixed, record；
Step 5, the mrna expression amount of breast tissue acceptor gene are determined
5.1 samplings
Nursing period East Lake generation sheep test group and sheep control group breast tissue sample are gathered with modus operandi, band in paraformaldehyde is placed in
Laboratory is gone back to, after fixed 48h, sample to 1cm is repaiied3；
5.2 sample preparations
5.2.1 be dehydrated by the tissue sample after fixation be sequentially placed into 70% alcohol 30min, 80% alcohol 1h, 90% alcohol 1h,
100% alcohol 1h, changes an alcohol after 1h；
5.2.2 it is transparent with etc. than row prepare absolute ethyl alcohol, dimethylbenzene mixed solution soak 2h, then with dimethylbenzene I immersion
10min, changes dimethylbenzene, then steep 5min；
5.2.3 waxdip soaks 2h in dimethylbenzene, the mixed liquor of paraffin liquid prepared than row is waited, and 2h, paraffin II liquid are soaked in paraffin I liquid
Middle leaching 2h, is subsequently placed in drying in 60 DEG C of baking boxs；
5.2.4 embed and preheating embedding medium is poured into Simple carton, then the tissue sample after waxdip is placed on and is stood at room temperature；
5.2.5 cut into slices and embedded wax stone is placed on paraffin slicing machine, be cut into 5um thin slices, then extend piece, bonding die, mark
Afterwards, it is placed in slide holding frame, is dried under the conditions of 37 DEG C；
5.3 dyeing
(1) paraffin section is placed in dimethylbenzene I liquid 10min, dimethylbenzene II liquid 5min, 100% ethanol I liquid by HE dyeing dewaxing successively
3min, 100% ethanol II liquid 3min, 90% ethanol 3min, 80% ethanol 3min, 70% ethanol 3min；
(2) dyeing brazilwood extract dyeing 2min, washing removes dye liquor, plus 5% glacial acetic acid differentiation 30s, washes 5min with distillation, then use
Eosin stains 1min, then examines under a microscope；
(3) decolourize for slide to be sequentially placed into 70% alcohol, 80% alcohol, 90% alcohol, 100% alcohol I liquid, 100% alcohol II
Decolourize each 1min in liquid, then respectively in dimethylbenzene I liquid and dimethylbenzene II liquid decolourings 5min；
(4) mounting takes out section, dries dimethylbenzene, and natural gum in drop, covered is dried at ambient temperature；
(5) take a picture：After being taken a picture with microscopic camera, graphical analysis is carried out；
5.4 data statistics and analysis
Dyeed by HE and East Lake generation sheep and sheep mammary structure are compared.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109526872A (en) * | 2019-01-14 | 2019-03-29 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of breeding method of Dual-purpose production of milk and meat sheep new varieties |
| CN115176761A (en) * | 2022-08-02 | 2022-10-14 | 西北农林科技大学 | Method for breeding new variety of Xinong milk sheep |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103404484A (en) * | 2013-08-30 | 2013-11-27 | 洛宁农本畜牧科技开发有限公司 | Dual-purpose sheep new strain cultivation method |
| CN103642800A (en) * | 2013-11-19 | 2014-03-19 | 上海交通大学医学院附属第九人民医院 | siRNA molecule composition, and applications thereof in treatment of hypertrophic scars |
-
2017
- 2017-02-17 CN CN201710086939.8A patent/CN106818639A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103404484A (en) * | 2013-08-30 | 2013-11-27 | 洛宁农本畜牧科技开发有限公司 | Dual-purpose sheep new strain cultivation method |
| CN103642800A (en) * | 2013-11-19 | 2014-03-19 | 上海交通大学医学院附属第九人民医院 | siRNA molecule composition, and applications thereof in treatment of hypertrophic scars |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109526872A (en) * | 2019-01-14 | 2019-03-29 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of breeding method of Dual-purpose production of milk and meat sheep new varieties |
| CN109526872B (en) * | 2019-01-14 | 2021-07-20 | 中国农业科学院兰州畜牧与兽药研究所 | Breeding method of new variety of meat and milk sheep |
| CN115176761A (en) * | 2022-08-02 | 2022-10-14 | 西北农林科技大学 | Method for breeding new variety of Xinong milk sheep |
| CN115176761B (en) * | 2022-08-02 | 2023-09-15 | 西北农林科技大学 | Cultivation method of new variety of milk sheep |
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