CN106806931A - A kind of starch compound hemostatic dressing of mesoporous silicon dioxide micro-sphere - Google Patents
A kind of starch compound hemostatic dressing of mesoporous silicon dioxide micro-sphere Download PDFInfo
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- CN106806931A CN106806931A CN201510868844.2A CN201510868844A CN106806931A CN 106806931 A CN106806931 A CN 106806931A CN 201510868844 A CN201510868844 A CN 201510868844A CN 106806931 A CN106806931 A CN 106806931A
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Abstract
The present invention relates to a kind of starch compound hemostatic dressing of mesoporous silicon dioxide micro-sphere, using sol-gel process, with triblock copolymer pluronic as template, mesoporous silicon oxide microballoons of the synthetic aperture in 5nm or so, it is combined with starch, mesoporous silicon oxide microballoons/starch compound hemostatic dressing is prepared, by the anthemorrhagic performance of animal experimental observation composite.Mesoporous silicon oxide microballoons/starch composite material is small only to have very strong water absorbing properties, but also with obvious external coagulant property, can be significantly reduced partial thromboplastin time and prothrombin time.Mesoporous silicon oxide microballoons/starch composite material can prevent bleeding and shortening its stream tertiary time for rabbit back skin and damage of the liver, with obvious haemostatic effect.
Description
Technical field
The invention belongs to styptic technical field, and in particular to a kind of starch compound hemostatic dressing of mesoporous silicon dioxide micro-sphere.
Background technology
Can various unexpected incidents, human body traumatic bleeding be very common, allow the surface of a wound bled fast and effectively to stop in a short time
Blood is the focus of current research.In recent years, hemostatic material in medical use causes medical field and the but change of industrial circle is paid attention to, many large-scale
Pharmaceuticals is devoted to the new hemostatic material of research and development.U.S. army is proposed " rapid hardening " tourniquet bandage in the Gulf War, it is said that can
Effectively reduce blood loss and reach 50%-80%, the use of the purpose of bleeding-stopping dressing is that the vessel retraction for making fracture is closed in tissue surface.Mesh
It is preceding that oneself develops the wound hemostatic material of numerous species.Mainly have:Fibrin Glue, collagen, shitosan, shuttle Methyl cellulose
Element, inorganic microporous material such as zeolite etc..
Why inorganic oxide zeolite haemostatic QuikClot has good anthemorrhagic performance, and being primarily due to it has big ratio
Surface area (about 300m2/g) can from blood a large amount of water of quick adsorption promoting coagulating platelets.But zeolite haemostatic has one very
Big not foot point, while water is adsorbed, can discharge big calorimetric, cause wound location temperature to rise sharply and the wounded is felt pain
And cause to burn.Experiment sol-gel process has synthesized mesoporous silicon oxide microballoons, and it is combined with starch, prepares mesopore silicon oxide
/ starch composite material bleeding-stopping dressing；Using partial thromboplastin time, ((APTT) and prothrombin time (PT), carrys out test compound material
Expect the influence to intrinsic coagulation system and extrinsic coagulation system；By animal experimental observation mesopore silicon oxide/starch composite material
Anthemorrhagic performance.
The content of the invention
It is an object of the invention to provide a kind of starch compound hemostatic dressing of mesoporous silicon dioxide micro-sphere.
A kind of starch compound hemostatic dressing of mesoporous silicon dioxide micro-sphere, its preparation method is：
The preparation of mesoporous silicon oxide microballoons：With tetraethyl orthosilicate (lower EOS) as silicon source, triblock copolymer P123 is as organic formwork
Agent, hydrochloric acid are catalyst, and mesoporous silicon oxide microballoons are prepared using sol-gel process.4g P123 are dissolved in 120mL 2mol/L
Extremely clarified in HCI and the mixed solution of 30mL deionized waters.9.2mL TEOS are added, dissolving is clarified, at a temperature of 50 DEG C,
Stirring 2h, forms the colloidal sol of stable transparent.Then colloidal sol is placed in 37 DEG C of constant temperature oscillation casees and is aged, dries, finally again with 1 DEG C
The programming rate of min is heated to 600 DEG C, and keeps 600 DEG C of temperature satin to burn material, obtains product.
It is an object of the invention to provide a kind of starch compound hemostatic dressing of mesoporous silicon dioxide micro-sphere.Zeolite
(QUICKCLOtTM) styptic has haemostatic effect, when being characterized in direct effect wound, with selective absorbing blood
Hydrone, without other compositions in absorption blood, causes the concentration of blood platelet and clotting factor, so as to reach the purpose of hemostasis,
The current commodity oneself through widely using at home, the mesopore silicon oxide/starch composite material of this experiment preparation has similar to the material
Anthemorrhagic performance.Test result indicate that, mesoporous oxidation alkali starch composite material is a kind of excellent bleeding-stopping dressing of haemostatic effect, can
For trauma hemostasis.Traditional Hemostasis such as pressurize, dressing filling and tourniquet, are stopped blooding by pressure, tourniquet
Hemostasis can also cause remote organization's ischemic and the metabolic disorder to cause serious complication.Mesopore silicon oxide/starch composite material not only stops
Blood is rapid, and efficiency high is easy to operate, and mesh unlike tourniquet to block remote organization's blood flow as cost, it is ensured that distal end
Limbs have blood supply.Therefore, mesoporous oxidation alkali starch composite material is suitable for war wound emergency treatment and wound quick-acting haemostatic powder usually.
Specific embodiment
Below by specific embodiment, the invention will be further described, and following examples are descriptive, be not it is limited,
Protection scope of the present invention can not be limited with this.
A kind of starch compound hemostatic dressing of mesoporous silicon dioxide micro-sphere, its preparation method is：
The preparation of mesoporous silicon oxide microballoons：With tetraethyl orthosilicate (lower EOS) as silicon source, triblock copolymer P123 is as organic formwork
Agent, hydrochloric acid are catalyst, and mesoporous silicon oxide microballoons are prepared using sol-gel process.4g P123 are dissolved in 120mL 2mol/L
Extremely clarified in HCI and the mixed solution of 30mL deionized waters.9.2mL TEOS are added, dissolving is clarified, at a temperature of 50 DEG C,
Stirring 2h, forms the colloidal sol of stable transparent.Then colloidal sol is placed in 37 DEG C of constant temperature oscillation casees and is aged, dries, finally again with 1 DEG C
The programming rate of/min is heated to 600 DEG C, and keeps 600 DEG C of temperature satin to burn material, obtains product.
Similarity condition synthesis is not added with the non-hole silica sample of organic formwork agent as control.
The preparation of mesopore silicon oxide/starch composite material and water absorbing properties:By the mesopore silicon oxide sample grind into powder after sintering.With
Physisorption Analyzer determines the different desorption curves of relative pressure P/Po h N2 isothermal adsorptions one, is calculated by under day E, analysis
The specific surface area and pore volume of material, average pore size is calculated according to Barrett-Joyner-Helen (BJH) formula；Take appropriate mesoporous oxygen
SiClx powder sample ultrasonic disperse in absolute ethyl alcohol, drips to carbon and supports on film with dropper by the ethanol containing sample, air drying
Afterwards, with the microstructure and pattern of transmission electron microscope analysis meso pore silicon oxide material.
Mesopore silicon oxide is mixed with starch powder, mesopore silicon oxide/starch composite material is prepared, by mesopore silicon oxide and its compound
Material sample conducting adhesive cloth is bonded on copper sample stage, is foretold to sample metal spraying in vacuum condition.SEM is observed
The microscopic appearance of material；The water absorption of mesopore silicon oxide/starch composite material can be tested:Claim quality 5g dry multiple with electronic balance
Condensation material (mo), material is put into small beaker, adds deionized water, after 2min, unnecessary moisture is suctioned out, after weighing water suction
Mesoporous material quality (mwet)
Every kind of sample repeats to survey 3 times, results averaged.Control sample mesopore silicon oxide and non-hole silica material.
The external coagulant property of mesopore silicon oxide/starch composite material：Mesoporous oxidation is observed using two coagulation indexes of APTT and PT
The preparation method of influence SD rat plasma of the silicon/starch composite material to blood coagulation system:After (4 μ L/g) rat being anaesthetized with urethane,
Blood is extracted from abdominal aorta, and adds anti-coagulants calcium citrate (0.109mol/L, 1 in time:9) in case Hemostatic Oral Liquid condenses,
4 DEG C, 3000r/min conditions foretell refrigerated centrifuge 10min and ratio of the platelet poor plasma according to experimental design are obtained, and in vitro add
Enter platelet poor plasma and mesopore silicon oxide/starch composite material powder, after both are well mixed rapidly, in 37 DEG C of incubators
Culture, adds calcium chloride solution, examines and records and the moment of fiber protein yarn occurs, when the time is partial thromboplastin
Between.The practice ibid, according to the ratio of experimental design, in vitro adds platelet poor plasma and mesopore silicon oxide/starch composite wood
Feed powder end, rapidly joins reagent under P, is placed in 37 DEG C of incubator cultures, the time required to shaking up and recording solidification rapidly, the portion
It is blood coagulation zymogen times between timesharing.With mesopore silicon oxide and non-hole silica material and the blank group of material is not added to compare.
Skin hemostasis experiment:It is standby cotton balls, mesopore silicon oxide/starch composite material and each several pieces of control sample to be weighed with electronic balance
With.New zealand white rabbit is fixed in experimental box, is not anaesthetized.Laterally drawn with blade as Test sites at selection White Rabbit back
Skin is opened, above-mentioned mesopore silicon oxide/starch composite material powder is sprinkling upon on wound rapidly, and with cotton balls flicking certain hour, removed
After removing pressure, if being hemostasis without oozing of blood around cotton balls, bleeding stopping period is determined with stopwatch.
Liver hemostasis experiment:Cotton balls, mesopore silicon oxide/starch composite material powder and each several pieces of control sample are weighed with electronic balance
It is standby.New zealand white rabbit is fixed in experimental box, is not anaesthetized.Selection White Rabbit belly uses blade transverse direction as Test sites
Skin is scratched, above-mentioned mesopore silicon oxide/starch composite material powder is sprinkling upon on liver wound rapidly, and with cotton balls flicking-fix time,
After removing pressure, if being hemostasis without oozing of blood around cotton balls, bleeding stopping period is determined with stopwatch.
MAIN OUTCOME MEASURES：The anthemorrhagic performance of mesopore silicon oxide/starch composite material.
Statistical analysis：Statistical disposition is completed using the softwares of SPSS 17.0 by the first authors.
2 results
Mesoporous pattern and pore size transmission electron microscope observation the display mesoporous silicon oxide microballoons of 2.1 materials have cellular
Pore passage structure feature, duct aligned orderly, diameter about in 5nm, this (pore-creating exactly with triblock copolymer P123 as template
Agent) formed mesopore orbit shape characteristic.
The pore-size distribution of material is narrow (5.0nm or so).Calculate and can draw from BE fourths and BJH:Mesoporous silicon oxide microballoons
Ratio table and product be 463m2/g, average pore size is 5.1nm, basically identical with TEM results.The pore size of porous material
Species with pore-foaming agent is closely related, with the mesoporous material aperture that triblock copolymer is templated synthesis in the range of 2-8nm.Cause
This, the size and pattern of mesoporous pore size can be controlled by selecting the species of template, so as to synthesize required material.
The microscopic appearance mesoporous silicon oxide microballoons of 2.2 materials are uniform in size, at 0.1 μm or so；And mesoporous silicon oxide microballoons/starch
Composite is then gel, and starch powder is wrapped in mesoporous silicon oxide microballoons wherein, forms gelatinous material, and oneself passes through
It is difficult to distinguish mesoporous silicon oxide microballoons from composite.
The water absorbing properties test result of 2.3 materials has very strong water absorbing properties compared to non-hole silica, mesopore silicon oxide, inhales
Water rate is 69%, rather than hole silica is only 18%, and this is accumulated and pore volume because mesopore silicon oxide has very big ratio table, is made
Material has very strong water absorbing properties.In addition, for mesopore silicon oxide/starch composite material, due to adding starch, composite wood
The water absorbing properties of material also have than mesopore silicon oxide and significantly improve；Compared to non-hole silica, mesopore silicon oxide, mesopore silicon oxide/
Preferably, water absorption rate is 81% to the water absorbing properties of starch composite material.Result shows:Starch is added to be conducive to raising mesopore silicon oxide micro-
The water absorbing properties of ball.
2.4 solidifying mountain experiments in vitro are compared with non-hole silica, and the AP of mesopore silicon oxide is lower to significantly reduce (contracting with the blood coagulation times under P
It is short), this is that energy is rapid because mesopore silicon oxide has specific surface area and pore volume very high, thus material with very strong absorption affinity
Moisture in blood is absorbed, causes pachyemia, blood is produced condensation, thus mesopore silicon oxide has good coagulant property,
Rather than the AP of hole silica is lower substantially long with the P lower clotting times.
For mesopore silicon oxide/starch composite material, due to adding starch, starch also has very strong water absorbing properties.So,
Compared with mesopore silicon oxide, APTT the and PT reduced clotting times of mesopore silicon oxide/starch composite material substantially, are as a result illustrated:
Adding starch can shorten the composite external clotting time.
The zoopery of 2.5 mesopore silicon oxides/starch composite material hemostasis
Skin stops blooding：After mesopore silicon oxide/starch composite material contact blood, moisture in blood can be rapidly absorbed, cause blood
Concentration, makes blood produce condensation, so as to promote hemostasis.
Rabbit back skin wound times of bleeding compare (after covering wound with mesopore silicon oxide/starch composite material), non-hole silica (S)
It is pair to be compared with non-hole silica (187s) with mesopore silicon oxide (m-S), the wounds streamed blood time after mesopore silicon oxide covering substantially subtracts
It is few, it is 78s, this is, because mesopore silicon oxide has very big specific surface area and pore volume, to promote hemostasis.After adding starch,
The wounds streamed blood time after mesopore silicon oxide/starch composite material covering significantly reduces, and is 64s, illustrates to add starch to improve again
The anthemorrhagic performance of condensation material.Result shows:Mesoporous oxidation alkali starch composite material is a kind of excellent biomaterial of haemostatic effect.
Liver stops blooding：After mesopore silicon oxide/starch composite material contact blood, fast photograph is shown in that figure speed forms blood clot, be due to
Mesopore silicon oxide/starch composite material absorbs rapidly moisture in blood, the relative concentration that improve blood coagulation substrate, solidifying so as to accelerate
Blood reaction speed；Form blood clot and be also beneficial to hemostasis.
Mesopore silicon oxide/starch composite material is used for the zoopery of only rabbit liver wounds streamed blood, non-hole silica and mesopore silicon oxide
It is control sample.Compared with non-hole silica (152s), the wounds streamed blood time after mesopore silicon oxide covering significantly reduces, and is 71s,
This is, because mesopore silicon oxide has very big specific surface area and pore volume, to promote hemostasis.After adding starch, mesopore silicon oxide/shallow lake
The wounds streamed blood time after powder composite material covering significantly reduces, and is 62s, illustrates the hemostasis for adding starch to improve composite
Performance.Result shows:Mesopore silicon oxide/starch composite material has good haemostatic effect.
Claims (1)
1. the starch compound hemostatic dressing of a kind of mesoporous silicon dioxide micro-sphere, it is characterised in that：Its preparation method is：
The preparation of mesoporous silicon oxide microballoons：With EOS as silicon source, triblock copolymer P123 as organic formwork agent, hydrochloric acid is to urge
Agent, mesoporous silicon oxide microballoons are prepared using sol-gel process.4g P123 are dissolved in 120mL 2mol/L HCI and 30mL
To clarification in the mixed solution of deionized water.9.2mL TEOS are added, dissolving clarification at a temperature of 50 DEG C, stirs 2h, shape
Into the colloidal sol of stable transparent.Then colloidal sol is placed in 37 DEG C of constant temperature oscillation casees and is aged, dries, finally again with 1 DEG C of liter of min
Warm speed is heated to 600 DEG C, and keeps 600 DEG C of temperature satin to burn material, obtains product.
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| CN108339148A (en) * | 2018-02-12 | 2018-07-31 | 华东理工大学 | A kind of meso-porous titanium dioxide silicon substrate complex microsphere and preparation method thereof with efficient coagulation function |
| CN108653791A (en) * | 2018-05-22 | 2018-10-16 | 东莞市联洲知识产权运营管理有限公司 | A kind of preparation method of the silica medical dressing based on aerosol deposition |
| CN109078216A (en) * | 2018-09-12 | 2018-12-25 | 宁夏悦丰生态农业科技有限公司 | Mesoporous silicon dioxide micro-sphere, bletilla polysaccharide compound hemostatic material and preparation method thereof |
| CN109498833A (en) * | 2018-12-12 | 2019-03-22 | 济南格莱威医疗科技有限公司 | A kind of Medical absorbable polysaccharide composite material and application thereof |
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| CN108339148A (en) * | 2018-02-12 | 2018-07-31 | 华东理工大学 | A kind of meso-porous titanium dioxide silicon substrate complex microsphere and preparation method thereof with efficient coagulation function |
| CN108653791A (en) * | 2018-05-22 | 2018-10-16 | 东莞市联洲知识产权运营管理有限公司 | A kind of preparation method of the silica medical dressing based on aerosol deposition |
| CN108653791B (en) * | 2018-05-22 | 2021-06-08 | 山东景天堂药业有限公司 | Preparation method of silica medical dressing based on aerosol deposition |
| CN109078216A (en) * | 2018-09-12 | 2018-12-25 | 宁夏悦丰生态农业科技有限公司 | Mesoporous silicon dioxide micro-sphere, bletilla polysaccharide compound hemostatic material and preparation method thereof |
| CN109498833A (en) * | 2018-12-12 | 2019-03-22 | 济南格莱威医疗科技有限公司 | A kind of Medical absorbable polysaccharide composite material and application thereof |
| CN111388739A (en) * | 2020-01-06 | 2020-07-10 | 太原理工大学 | Nano silicon dioxide/decomposition enzyme/polycaprolactone composite microsphere and preparation method and application thereof |
| CN112245645A (en) * | 2020-09-01 | 2021-01-22 | 世纪亿康（天津）医疗科技发展有限公司 | Hemostatic and antibacterial medical dressing and preparation method thereof |
| CN112587707A (en) * | 2020-12-29 | 2021-04-02 | 福建恒安家庭生活用品有限公司 | Absorption core body with blood separation function and sanitary product |
| CN112826666A (en) * | 2020-12-29 | 2021-05-25 | 福建恒安卫生材料有限公司 | Deodorization absorption core and sanitary product |
| CN112587707B (en) * | 2020-12-29 | 2022-07-05 | 福建恒安家庭生活用品有限公司 | Absorption core body with blood separation function and hygienic product |
| CN113975454A (en) * | 2021-11-12 | 2022-01-28 | 延边大学 | Preparation and application of mesoporous silica/tannic acid composite hydrogel hemostatic material |
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