CN106804581A - A kind of porcine semen at normal temperature liquid negative pressure save set and method - Google Patents
A kind of porcine semen at normal temperature liquid negative pressure save set and method Download PDFInfo
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- CN106804581A CN106804581A CN201710085421.2A CN201710085421A CN106804581A CN 106804581 A CN106804581 A CN 106804581A CN 201710085421 A CN201710085421 A CN 201710085421A CN 106804581 A CN106804581 A CN 106804581A
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- semen
- closed container
- vavuum pump
- connecting tube
- rubber stopper
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0289—Pressure processes, i.e. using a designated change in pressure over time
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
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- General Health & Medical Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of porcine semen at normal temperature liquid storage device of the present invention, including closed container, rubber stopper, connecting tube, vavuum pump and Semen routine pipe, closed container top is provided with rubber stopper, stretched into inside closed container through rubber stopper one end of connecting tube, the other end connects vavuum pump, and Semen routine pipe is placed in closed container inner bottom part.The invention further relates to a kind of method that utilization said apparatus preserve pig semen, comprise the following steps：Fresh pig semen is removed into upper strata refining through centrifuge, it is diluted with dilution then.Semen routine pipe is put into closed container, the rubber stopper with valve is covered, and connecting tube is connected with the vavuum pump with vacuum meter；Start vavuum pump, the air in closed container is extracted out.By the present invention in that carrying out porcine semen at normal temperature liquid storage with the present apparatus, the holding time can be extended to 9 13 days, sperm and semen quality are significantly improved.
Description
Technical field
The present invention relates to technical field of animal reproduction, and in particular to a kind of porcine semen at normal temperature liquid negative pressure save set
And method.
Background technology
In recent years, continuing to develop with pig industry, the technology of artificial insemination of pig has also obtained swift and violent development, but due to people
Seminal fluid needed for work insemination is at present or main based on normal temperature liquid storage, and the quality and timeliness of pig semen liquid storage turn into
Restrict the key issue of Artificial Insemination Technology of Swine.At present, there is holding time short (general preservation in the seminal fluid of pig normal temperature liquid storage
3-7 days), preserve after the problems such as sperm motility is low, abnormal rate is high.There is researcher to attempt to enter Diluent of Pig's Spermatic Fluid component prescription
Row improvement is to extending the time of pig semen preservation and improving semen quality, but effect is undesirable.So be badly in need of one kind now prolonging
The time of pig semen liquid storage long and the apparatus and method for improving semen quality.
The content of the invention
The invention aims to solve in the prior art, the porcine semen at normal temperature liquid storage time is short, and sperm motility is low
Ropy problem, and then a kind of porcine semen at normal temperature liquid negative pressure save set and method are provided.
A kind of porcine semen at normal temperature liquid storage device of the invention, including closed container 1, rubber stopper 2, connecting tube 4, vacuum
Pump 5 and Semen routine pipe 6, the top of closed container 1 are provided with rubber stopper 2, and closed appearance is stretched into one end of connecting tube 4 through rubber stopper 2
Inside device 1, other end connection vavuum pump 5, Semen routine pipe 6 is placed in the inner bottom part of closed container 1.
Further, connecting tube 4 is provided with valve 3.
The invention further relates to a kind of method that utilization said apparatus preserve pig semen, comprise the following steps：
Step one, by fresh pig semen through centrifuge remove upper strata refining, it is diluted with dilution then, will be dense
Degree is deployed into every milliliter 1 × 108~5 × 108It is individual, then detect sperm motility therein, it is up to standard after be placed in Semen routine pipe 6；
Step 2, Semen routine pipe 6 is put into closed container 1, covers the rubber stopper 2 with valve, and by connecting tube 4 with
Band vavuum pump 5 connect, start vavuum pump 5 by closed container 1 air extract out, make air pressure be reduced to -0.04MPa~-
0.08MPa, the switch of vavuum pump 5 separates connecting tube 4 with closed container 1；
Step 3, closed container 1 is put into 16~18 DEG C of incubators and is preserved.
The present invention has the advantages that：The present invention carries out porcine semen at normal temperature liquid using the environment that the present apparatus provides negative pressure
State is preserved, and can be extended to the holding time 9-13 days, and sperm and semen quality are significantly improved, and present invention operation
Simply, efficiency high, it is easy to use, it is adapted to porcine semen at normal temperature fluid preservation and obtains popularization and application.
Brief description of the drawings
Fig. 1 is the structural representation of porcine semen at normal temperature liquid storage device of the present invention.
It is described as follows：
Closed container 1, rubber stopper 2, valve 3, connecting tube 4, vavuum pump 5, Semen routine pipe 6.
Specific embodiment
The present invention relates to a kind of porcine semen at normal temperature liquid storage device, the device includes closed container 1, rubber stopper 2, connection
Pipe 4 and vavuum pump 5, the top of closed container 1 are provided with rubber stopper 2, and one end of connecting tube 4 is stretched into closed container 1 through rubber stopper 2
Portion, other end connection vavuum pump 5.Semen routine pipe 6 is placed in the bottom of closed container 1, and connecting tube 4 is provided with valve 3.
Said apparatus are mainly used in the preservation of pig semen, and specific store method comprises the following steps：
Step one, by fresh pig semen through centrifuge remove upper strata refining, it is diluted with dilution then, will be dense
Degree is deployed into every milliliter 1 × 108~5 × 108It is individual, then detect sperm motility therein, it is up to standard after be placed in Semen routine pipe 6；Institute
Semen routine pipe 6 is stated to add a cover, but blow-by.
Step 2, Semen routine pipe 6 is put into closed container 1, covers the rubber stopper 2 with valve, and by connecting tube 4 with
Band vavuum pump 5 connect, start vavuum pump 5 by closed container 1 air extract out, make air pressure be reduced to -0.04MPa~-
0.08MPa, the switch of vavuum pump 5 separates connecting tube 4 with closed container 1；
Step 3, closed container 1 is put into 16~18 DEG C of incubators and is preserved.
Preferably, in step one, rotating speed when centrifuge works is 1800r/ minutes, and centrifugation time is 5 minutes, essence
The volume that liquid preserves pipe 6 is 15~80ml.Dilution is Modena dilutions, wherein the bovine serum albumin(BSA) containing 4mg/ml.
Embodiment 1 (experimental group)
The method that pig semen is preserved in the present embodiment, comprises the following steps：
Step one, by fresh pig semen through centrifuge remove upper strata refining, centrifuge work when rotating speed be
1800r/ minutes, centrifugation time was 5 minutes.Then it is diluted with Modena dilutions, concentration is deployed into every milliliter 1
×108~5 × 108It is individual, then detect sperm motility therein, it is up to standard after take 50 milliliters and be placed in Semen routine pipe 6；
Step 2, Semen routine pipe 6 is put into closed container 1, covers the rubber stopper 2 with valve, and by connecting tube 4 with
Vavuum pump 5 with vacuum meter is connected；Start vavuum pump 5, the air in closed container 1 extracted out, air pressure is reduced to -0.04MPa,
The switch of valve 3 and vavuum pump 5 is closed, connecting tube 4 is separated with closed container 1；
Step 3, closed container 1 is put into 17 DEG C of pig semen incubators to be preserved and obtain required pig semen.
Embodiment 2 (control group)
The method that pig semen is preserved in the present embodiment, comprises the following steps：
Step one, by fresh pig semen through centrifuge remove upper strata refining, centrifuge work when rotating speed be
1800r/ minutes, centrifugation time was 5 minutes.Then it is diluted with Modena dilutions, concentration is deployed into every milliliter 1
×108It is individual, then detect sperm motility therein, it is up to standard after take 50 milliliters and be placed in Semen routine pipe 6；
Step 2, Semen routine pipe 6 is put into 17 DEG C of pig semen incubators to be preserved and obtain required pig semen.
Contrast test
For, to the different store methods of pig semen, test operation being carried out respectively in embodiment 1 and embodiment 2.In difference
Preservation number of days under, the number of sperm motility, abnormal rate, pH value, active oxygen and the TAC of pig semen is determined respectively
Value, data are as shown in table 1 below to 5 (in table 1 to 5, with the female difference significant difference of column data shoulder marking-up, P<0.05)：
Influence of the different preserving types of table 1. to the Boar spermatozoa vigor of preservation different time
The different preserving types of table 2. influence on the Boar spermatozoa abnormal rate for preserving different time
Influence of the different preserving types of table 3. to the pig semen pH of preservation different time
Influence of the different preserving types of table 4. to the pig semen active oxygen (ROS) of preservation different time
Boar spermatozoa TAC (T-AOC) (μm ol/ of the different preserving types of table 5. to preservation different time
107Sperm influence)
Analysis：
As can be seen from the above data, the pig semen for being preserved under normal temperature negative pressure over time, its sperm motility is gradually excellent
In the pig semen preserved under normal temperature and pressure, and abnormal rate is substantially reduced compared with the latter.PH value and total antioxidation numerical value are normal better than normal temperature
Numerical value under the conditions of pressure, active oxygen numerical value is less than the latter.Main cause is that negative pressure reduces oxygen content, sperm is in dormancy
State, its metabolism slow down after, extend the holding time.Sperm breathing produces CO2So that the pH value of seminal fluid, during with preserving
Between extension be gradually reduced.Weak acid environment can suppress sperm motility and metabolism, and negative pressure can slow down the decline of PH.From sperm
Oxidation resistance aspect for, negative pressure preserve it is stronger than the inoxidizability of the sperm under normal pressure, it can be seen that from after 3 days
There were significant differences.
Explained by the analysis to above-mentioned data and principle, it can be seen that the present invention provides the ring of negative pressure using vavuum pump
Border, recycles the principle of negative pressure self-priming, and porcine semen at normal temperature liquid negative pressure is preserved, and can extend the holding time (9-13 days),
Sperm and semen quality are significantly improved.
The above is only presently preferred embodiments of the present invention, is not intended to limit embodiment of the present invention, and this area is general
Logical technical staff's central scope of the invention and spirit, can very easily carry out corresponding flexible or modification, therefore originally
The protection domain of invention should be defined by the protection domain required by claims.
Claims (7)
1. a kind of porcine semen at normal temperature liquid storage device, it is characterised in that described device includes closed container (1), rubber stopper
(2), connecting tube (4), vavuum pump (5) and Semen routine pipe (6), closed container (1) top is provided with rubber stopper (2), connecting tube (4)
One end stretch into that closed container (1) is internal through rubber stopper (2), the other end connects vavuum pump (5), and Semen routine pipe (6) is placed in
Closed container (1) inner bottom part.
2. porcine semen at normal temperature liquid storage device according to claim 1, it is characterised in that connecting tube (4) is provided with valve
Door (3).
3. the method that a kind of device of utilization the claims 1 preserves pig semen, it is characterised in that comprise the following steps：
Step one, by fresh pig semen through centrifuge remove upper strata refining, it is diluted with dilution then, concentration is adjusted
It is made into every milliliter 1 × 108~5 × 108It is individual, then detect sperm motility therein, it is up to standard after be placed in Semen routine pipe (6)；
Step 2, Semen routine pipe (6) is put into closed container (1), covers the rubber stopper (2) with valve, and by connecting tube
(4) it is connected with band vavuum pump (5), (5 extract the air in closed container (1) out, air pressure is reduced to -0.04MPa to start vavuum pump
~-0.08MPa, the switch of vavuum pump (5) separates connecting tube (4) with closed container (1)；
Step 3, closed container 1 is put into 16~18 DEG C of incubators and is preserved.
4. it is according to claim 3 preserve pig semen method, it is characterised in that in step one, centrifuge work
When rotating speed be 1800r/ minute, centrifugation time be 5 minutes.
5. it is according to claim 3 preserve pig semen method, it is characterised in that in step one, Semen routine pipe (6)
Volume is 15~80ml.
6. the method for preserving pig semen according to claim 3, it is characterised in that in step one, the dilution is to rub
Moral receives dilution, wherein the bovine serum albumin(BSA) containing 4mg/ml.
7. it is according to claim 3 preserve pig semen method, it is characterised in that in step one, the Semen routine pipe
(6) add a cover, but blow-by.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710085421.2A CN106804581B (en) | 2017-02-17 | 2017-02-17 | Device and method for preserving normal-temperature liquid-state pig semen under negative pressure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710085421.2A CN106804581B (en) | 2017-02-17 | 2017-02-17 | Device and method for preserving normal-temperature liquid-state pig semen under negative pressure |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106804581A true CN106804581A (en) | 2017-06-09 |
| CN106804581B CN106804581B (en) | 2020-08-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710085421.2A Active CN106804581B (en) | 2017-02-17 | 2017-02-17 | Device and method for preserving normal-temperature liquid-state pig semen under negative pressure |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112021306A (en) * | 2020-10-12 | 2020-12-04 | 吉林省农业科学院 | Device and method for preparing pig frozen semen diluent |
| CN114601540A (en) * | 2022-03-30 | 2022-06-10 | 中国人民解放军总医院第三医学中心 | A seminal fluid collection system for uropoiesis surgery |
| CN116439232A (en) * | 2023-06-14 | 2023-07-18 | 山东第一医科大学附属省立医院(山东省立医院) | Chest surgery pathological tissue collecting and preserving device |
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| CN204896271U (en) * | 2015-08-14 | 2015-12-23 | 重庆市六九原种猪场有限公司 | Transport means is stored to pig normal atmospheric temperature seminal fluid |
| CN205093446U (en) * | 2015-09-30 | 2016-03-23 | 东阿阿胶股份有限公司 | Semen storage transportation cup |
| CN205849585U (en) * | 2016-10-08 | 2017-01-04 | 四川农业大学 | A kind of constant temperature pig collection essence bottle |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112021306A (en) * | 2020-10-12 | 2020-12-04 | 吉林省农业科学院 | Device and method for preparing pig frozen semen diluent |
| CN114601540A (en) * | 2022-03-30 | 2022-06-10 | 中国人民解放军总医院第三医学中心 | A seminal fluid collection system for uropoiesis surgery |
| CN114601540B (en) * | 2022-03-30 | 2023-07-14 | 中国人民解放军总医院第三医学中心 | Semen collection device for urology surgery |
| CN116439232A (en) * | 2023-06-14 | 2023-07-18 | 山东第一医科大学附属省立医院(山东省立医院) | Chest surgery pathological tissue collecting and preserving device |
| CN116439232B (en) * | 2023-06-14 | 2023-09-29 | 山东第一医科大学附属省立医院(山东省立医院) | Chest surgery pathological tissue collecting and preserving device |
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| CN106804581B (en) | 2020-08-04 |
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