CN106795503A - 用于改善的蛋白质产生的组合物和方法 - Google Patents

用于改善的蛋白质产生的组合物和方法 Download PDF

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CN106795503A
CN106795503A CN201580055291.7A CN201580055291A CN106795503A CN 106795503 A CN106795503 A CN 106795503A CN 201580055291 A CN201580055291 A CN 201580055291A CN 106795503 A CN106795503 A CN 106795503A
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copper
host cell
enzyme
oxidase
expression
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Chinese (zh)
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S·M·马德里
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Danisco USA Inc
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Danisco USA Inc
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    • C12N15/09—Recombinant DNA-technology
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0059—Catechol oxidase (1.10.3.1), i.e. tyrosinase
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
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    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0063—Ascorbate oxidase (1.10.3.3)
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
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    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03005—Hexose oxidase (1.1.3.5)
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    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03009—Galactose oxidase (1.1.3.9)
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/99—Oxidoreductases acting on the CH-OH group of donors (1.1) with other acceptors (1.1.99)
    • C12Y101/99018—Cellobiose oxidase (1.1.99.18)
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    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/03—Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
    • C12Y103/03005—Bilirubin oxidase (1.3.3.5)
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    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/03—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12Y104/03002—L-Amino-acid oxidase (1.4.3.2)
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    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03001—Catechol oxidase (1.10.3.1), i.e. tyrosinase
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    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002—Laccase (1.10.3.2)
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    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03003—L-ascorbate oxidase (1.10.3.3)
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    • C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11024—Quercetin 2,3-dioxygenase (1.13.11.24)
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    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/17—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced ascorbate as one donor, and incorporation of one atom of oxygen (1.14.17)
    • C12Y114/17001—Dopamine beta-monooxygenase (1.14.17.1)
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    • C12Y114/17—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced ascorbate as one donor, and incorporation of one atom of oxygen (1.14.17)
    • C12Y114/17003—Peptidylglycine monooxygenase (1.14.17.3)
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    • C12Y114/18—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with another compound as one donor, and incorporation of one atom of oxygen (1.14.18)
    • C12Y114/18001—Tyrosinase (1.14.18.1)
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    • C12Y115/00—Oxidoreductases acting on superoxide as acceptor (1.15)
    • C12Y115/01—Oxidoreductases acting on superoxide as acceptor (1.15) with NAD or NADP as acceptor (1.15.1)
    • C12Y115/01001—Superoxide dismutase (1.15.1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source

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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
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  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201580055291.7A 2014-08-15 2015-08-14 用于改善的蛋白质产生的组合物和方法 Pending CN106795503A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462038095P 2014-08-15 2014-08-15
US62/038,095 2014-08-15
PCT/US2015/045260 WO2016025825A1 (en) 2014-08-15 2015-08-14 Compositions and methods for improved protein production

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CN106795503A true CN106795503A (zh) 2017-05-31

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US (1) US20170233746A1 (enExample)
EP (1) EP3180354A1 (enExample)
JP (1) JP2017525359A (enExample)
KR (1) KR20170036102A (enExample)
CN (1) CN106795503A (enExample)
WO (1) WO2016025825A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115786142A (zh) * 2022-11-22 2023-03-14 中国农业大学 一种高产酪氨酸酶2的里氏木霉工程菌株的构建方法和应用

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3027742B1 (en) 2013-07-29 2019-11-20 Danisco US Inc. Variant enzymes
KR20250132725A (ko) 2024-02-29 2025-09-05 청주대학교 산학협력단 무인수상정

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10046932A1 (de) * 2000-09-21 2002-05-16 Consortium Elektrochem Ind Expressionssystem zur Überexpression von Kupfer-abhängigen sekretierten Proteinen in eukaryontischen Zellen
US6747137B1 (en) * 1998-02-13 2004-06-08 Genome Therapeutics Corporation Nucleic acid sequences relating to Candida albicans for diagnostics and therapeutics
CN101842479A (zh) * 2007-11-01 2010-09-22 丹尼斯科美国公司 用于改良宿主细胞内蛋白质生产的信号序列和共表达的分子伴侣

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102939382B (zh) * 2010-06-03 2017-02-15 丹尼斯科美国公司 丝状真菌宿主菌株和dna构建体以及它们的使用方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6747137B1 (en) * 1998-02-13 2004-06-08 Genome Therapeutics Corporation Nucleic acid sequences relating to Candida albicans for diagnostics and therapeutics
DE10046932A1 (de) * 2000-09-21 2002-05-16 Consortium Elektrochem Ind Expressionssystem zur Überexpression von Kupfer-abhängigen sekretierten Proteinen in eukaryontischen Zellen
CN101842479A (zh) * 2007-11-01 2010-09-22 丹尼斯科美国公司 用于改良宿主细胞内蛋白质生产的信号序列和共表达的分子伴侣

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANDREAS ULDSCHMID等: "Identification and functional expression of tahA, a filamentous fungal gene involved in copper trafficking to the secretory pathway in Trametes versicolor", 《MICROBIOLOGY》 *
GREG KELLER等: "Independent Metalloregulation of Ace1 and Mac1 in Saccharomyces cerevisiae", 《EUKARYOTIC CELL》 *
无: "Genbank登录号：XP_ 006968226.1", 《GENBANK》 *
无: "Genbank登录号：XP_006965640.1", 《GENBANK》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115786142A (zh) * 2022-11-22 2023-03-14 中国农业大学 一种高产酪氨酸酶2的里氏木霉工程菌株的构建方法和应用

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US20170233746A1 (en) 2017-08-17
KR20170036102A (ko) 2017-03-31
JP2017525359A (ja) 2017-09-07
WO2016025825A1 (en) 2016-02-18

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Application publication date: 20170531