CN106771057B - A method of accurately detecting Lead Pollution in Soil degree based on Atrichum undulatum - Google Patents
A method of accurately detecting Lead Pollution in Soil degree based on Atrichum undulatum Download PDFInfo
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Abstract
The present invention relates to a kind of method for accurately detecting Lead Pollution in Soil degree based on Atrichum undulatum, this method includes the cultivation preparation method and the dust-proof detection method of double-layer isolated type soil pick-up of vegetable material；The cultivation preparation method of vegetable material includes：Prepare spore suspension；Prepare culture substrate；Condition of culture is set.The dust-proof detection method of double-layer isolated type soil pick-up utilizes plant of the double-layer isolated type soil pick-up dust guard covering for detecting as Lead Pollution in Soil.The technology of artificial culture and propagation Atrichum undulatum provided by the invention and " the double-deck soil pick-up dust cover " that the exclusion leaded pollutant effect of atmosphere can efficiently be isolated are applied to the implementation process of Atrichum undulatum detection Lead Pollution in Soil, it can be achieved that Atrichum undulatum height accurately detects Lead Pollution in Soil.
Description
Technical field
The invention belongs to detect heavy metal pollution of soil technical field using bryophyte, more particularly to it is a kind of based on wave leaf
The method that red crowned crane moss accurately detects Lead Pollution in Soil degree.
Background technique
Lead is a kind of vertical distribution of stronger potential risk of tool, has carcinogenic, teratogenesis to people and animals' biology, causes to dash forward
The toxic characteristic of change, it is more stable in the soil, it is not easy to be metabolized and degrade, but people and animals can be made by the bioconcentration of food chain
Slow poisoning occurs, therefore has attracted much attention.Since the yield and purposes of products containing lead are being continuously increased, according to statistics, Chinese only lead
The arable land of pollution just has 6000~80,000,000 mu, every year the lead as caused by industrial produced wastes, wherein 70%~80% meeting
It is discharged into soil；Also have quite high lead content in the petroleum fuel that largely uses at present, after atmospheric sedimentation, also plus
The lead contamination in soil is weighed.In addition, lead contamination has sizable irreversibility, soil is once contaminated, to crops
Influence will continue for an extended period of time.Chemical measure is mostly used to the evaluation method of Lead Pollution in Soil in the industry, i.e. measurement is unearthed
The content value of lead in earth, in this, as the foundation for measuring Pb in Soil ecological security.But produced by the lead element reality in soil
Toxicity and its biological effectiveness it is closely related, measure whether soil has reached safety standard, finally should be with the reality in soil
Border Lead Toxicity is to extent of injury caused by organism as judgment basis.From this, if having using certain to lead strong quick
Perception rudimentary plant, Lead Pollution in Soil is indicated by its upgrowth situation and physiological reaction, can have stronger intuitive and
Convincingness.In other words, it is impacted if leaded soil locating for the plant not yet grows it, illustrates that soil at this time is safety
, it is suitble to plant other crops；Otherwise illustrate that the soil has had exceeded lead contamination margin of safety, there is certain lead contamination
Risk and be unsuitable for plant crops.At present for Environmental Lead Exposure evaluation aspect, with it is more be bryophyte detection method,
But such method is detected in the detection being used in atmosphere lead contamination, but has no always and relates to the detection of soil.If by moss
Plnat monitoring method is applied to the detection of Lead Pollution in Soil, then needs to ask a question before solving：First is that how to eliminate in natural environment
How bryophyte of long term growth itself promotes detection material used to lead to background adaptation possessed by pollution environment
The sensibility of pollutant；Second is that how to avoid the influence of leaded dust particles in air, and keep the evil of lead poisoning suffered by moss complete
Soil of the portion from its growth.The effect of bryophyte detection Lead Pollution in Soil can fundamentally be improved by resolving above-mentioned two problem
Fruit and accuracy.
Summary of the invention
The present invention is to solve technical problem present in well-known technique and provide one kind and accurately detected based on Atrichum undulatum
The method of Lead Pollution in Soil degree, it is intended to solve how to obtain suitable detection material and how avoid leaded dust in air
The influence of grain, thus soil of the evil of lead poisoning suffered by the moss for keeping sensibility high all from its growth, and then in root
The effect and accuracy of bryophyte detection Lead Pollution in Soil are improved in sheet.
The present invention is adopted the technical scheme that solve technical problem present in well-known technique：
The method provided by the invention that Lead Pollution in Soil degree is accurately detected based on Atrichum undulatum, which is characterized in that the party
Method includes the cultivation preparation method and the dust-proof detection method of double-layer isolated type soil pick-up of vegetable material；
The cultivation preparation method of vegetable material：
Prepare spore suspension：The sporinite for acquiring spore growth phase mature Atrichum undulatum plant is several, is put in stone
It in English mortar, after a little liquid nitrogen is added, is lightly ground to the luxuriant capsule of sporinite and is completely severed, built-in spore is made to shed, be added
Washing lotion is passed through the stainless steel filtering net that cell inner diameter is 1mm together and is filtered, filters major part by distilled water repeated flushing mortar
Luxuriant capsule residuum, to obtain spore suspension；
Prepare culture substrate：Using the clean sandy soil and plant residue fermentation material that volume ratio is 1 ︰ 1 as matrix formulations, fill
Atrichum undulatum culture substrate is used as after dividing mixing；The culture substrate prepared is laid in the polyethylene of diameter 35cm, high 6cm
In tray, stromal thickness 5cm；
Condition of culture is set：Spore suspension is uniformly sprayed in culture substrate, artificial lighting weather incubator is placed in
Interior, the field environment of simulation emergence preceding four May starts to grow to Atrichum undulatum seedling；Then six July after simulation emergence
The field environment of part continues to cultivate；40~50d enters the spore growth stage after emergence, using the plant in the period as Pb in Soil
The detection material of pollution；
The dust-proof detection method of double-layer isolated type soil pick-up：Using the covering of double-layer isolated type soil pick-up dust guard for as soil
The plant of lead contamination detection；The double-layer isolated type soil pick-up dust guard is provided with the supporting support of cone shape, big by one
A small group at；By 4 iron wire connection top and bottom annulus on circular conical surface；Two sets of brackets whole body use food fresh keeping membrane or its
He wraps up thin and transparent material, and the part between two stands bottom is also even sealed with membrane material, the loading end as active carbon；
Inner support lower hollow is not closed；Hole is respectively kept in the whole body wrap film of inside and outside two stands, under exit orifice occupies, aperture is slightly larger,
On inner hole occupies, aperture is slightly smaller；In use, placing layer of active carbon on connecting the loading end between two stands bottom, then should
Bracket is integrally buckled on Atrichum undulatum plant.
Further, plant residue fermentation material is with the replacement of flowers retting fertilizer.
Further, spore suspension is uniformly sprayed in culture substrate, is placed in artificial lighting weather incubator, initially
Cultivation temperature is set as 22 DEG C~26 DEG C, and intensity of illumination is set as 2500Lux/10h, the daily Hogland battalion for spraying 1/6 concentration
Nutrient solution 300-400mL/ disk, makes soil humidity be maintained at 70%~80%；Atrichum undulatum seedling starts to grow after 20~30d,
32 DEG C~37 DEG C are set by cultivation temperature at this time, intensity of illumination is set as 5000Lux/14h；40~50d enters after emergence
The spore growth stage, using the plant in the period as the detection material of Lead Pollution in Soil.
Present invention employs a kind of bryophytes having not been reported, having high sensibility to the lead in soil ---
Atrichum undulatum, evolution degree is low, plant structure is simple, vulnerable to the Lead Toxicity in environment, is highly prone to soil
The murder by poisoning of middle lead simultaneously rapidly shows symptom, and this kind of plant is different from general tiny moss, plant in appearance
Body is relatively large, and height is fairly obvious by the symptom shown after lead poisoning evil, it is easy to be seen up to 4~6cm when mature
It surveys, therefore is very suitable to carry out Lead Pollution in Soil detection.What the present invention had carried out related explores the results show that no matter from the plant
Mode of appearance or the reaction of physical signs such as chlorophyll content, soluble protein content and MDA concentration from the point of view of, the plant
There is stronger sensing capability to the lead in soil, and each index variation degree has preferably corresponded to the lead contamination journey in soil
Degree, therefore these indexs are suitable as the actual parameter of Atrichum undulatum detection Lead Pollution in Soil.But it finds in specific application,
Direct collected Atrichum undulatum, decreases to the sensing capability of Lead Pollution in Soil, causes to detect from natural environment
Ineffective, tracing it to its cause is to produce certain adaptability to pollution environment during long term growth due to it, before
Specially treated its background adaptation of elimination is needed, to enhance its susceptibility to lead；It is moreover found that utilizing Atrichum undulatum
When carrying out Lead Pollution in Soil on-site test, local environment is generally open space, there is more multi-form float in air
State contains land pollutant, such as leaded particle, leaded dust, leaded particulate, the leaded dust particles of earth's surface are also easy to
It is emitted in lower atmosphere, and Atrichum undulatum is exposed to plant due to the covering protection of surface anoderm and wax coat, stomata
Object table (this is the place that higher plant is different from bryophyte structure), therefore above-mentioned gaseous state is highly susceptible to containing land pollutant
Intrusion poison, this be also all the time by bryophyte be applied to Atmosphere Lead pollution detection cardinal principle and foundation.Therefore
When using Atrichum undulatum detection Lead Pollution in Soil, it is difficult to determine that lead poisoning evil that it is subject to comes from soil also or greatly
Gas.The presence of above-mentioned two aspects factor reduces Atrichum undulatum to the detection effect and accuracy of Lead Pollution in Soil.It is based on
This, the present invention relates to a kind of technical methods for cultivating Atrichum undulatum, the process of Fresh Plants is regenerated by spore germination,
Its background adaptation to primitive environment is effectively eliminated, its susceptibility to Lead Pollution in Soil is further enhanced, with this
Obtain the plant material that can be used for efficient detection Lead Pollution in Soil；Leaded dirt in air can be effectively isolated the invention further relates to a kind of
The method for contaminating object, makes Atrichum undulatum when detecting Lead Pollution in Soil, and suffered lead poisoning is all from soil, further
Enhance the accuracy and feasibility of Atrichum undulatum detection Lead Pollution in Soil.Specification of the present invention bryophyte detection environment is dirty
The technical standard of dye has high application value and promotion prospect.
Detailed description of the invention
Fig. 1 is double-layer isolated type soil pick-up dust cover basic framework schematic diagram provided by the invention.
In figure：1, outrigger；2, internal frame；3, inner hole；4, exit orifice；5, active carbon layer.
Specific embodiment
In order to further understand the content, features and effects of the present invention, the following examples are hereby given, and cooperate attached drawing
Detailed description are as follows.
Present invention employs a kind of bryophytes having not been reported, having high sensibility to the lead in soil ---
Atrichum undulatum, evolution degree is low, plant structure is simple, vulnerable to the Lead Toxicity in environment, is highly prone to soil
The murder by poisoning of middle lead simultaneously rapidly shows symptom, and this kind of plant is different from general tiny moss, plant in appearance
Body is relatively large, and height is fairly obvious by the symptom shown after lead poisoning evil, it is easy to be seen up to 4~6cm when mature
It surveys, therefore is very suitable to carry out Lead Pollution in Soil detection.What the present invention had carried out related explores the results show that no matter from the plant
Mode of appearance or the reaction of physical signs such as chlorophyll content, soluble protein content and MDA concentration from the point of view of, the plant
There is stronger sensing capability to the lead in soil, and each index variation degree has preferably corresponded to the lead contamination journey in soil
Degree, therefore these indexs are suitable as the actual parameter of Atrichum undulatum detection Lead Pollution in Soil.But it finds in specific application,
Direct collected Atrichum undulatum, decreases to the sensing capability of Lead Pollution in Soil, causes to detect from natural environment
Ineffective, tracing it to its cause is to produce certain adaptability to pollution environment during long term growth due to it, before
Specially treated its background adaptation of elimination is needed, to enhance its susceptibility to lead；It is moreover found that utilizing Atrichum undulatum
When carrying out Lead Pollution in Soil on-site test, local environment is generally open space, there is more multi-form float in air
State contains land pollutant, such as leaded particle, leaded dust, leaded particulate, the leaded dust particles of earth's surface are also easy to
It is emitted in lower atmosphere, and Atrichum undulatum is exposed to plant due to the covering protection of surface anoderm and wax coat, stomata
Object table (this is the place that higher plant is different from bryophyte structure), therefore above-mentioned gaseous state is highly susceptible to containing land pollutant
Intrusion poison, this be also all the time by bryophyte be applied to Atmosphere Lead pollution detection cardinal principle and foundation.Therefore
When using Atrichum undulatum detection Lead Pollution in Soil, it is difficult to determine that lead poisoning evil that it is subject to comes from soil also or greatly
Gas.The presence of above-mentioned two aspects factor reduces Atrichum undulatum to the detection effect and accuracy of Lead Pollution in Soil.It is based on
This, the present invention relates to a kind of technical methods for cultivating Atrichum undulatum, the process of Fresh Plants is regenerated by spore germination,
Its background adaptation to primitive environment is effectively eliminated, its susceptibility to Lead Pollution in Soil is further enhanced, with this
Obtain the plant material that can be used for efficient detection Lead Pollution in Soil；Leaded dirt in air can be effectively isolated the invention further relates to a kind of
The method for contaminating object, makes Atrichum undulatum when detecting Lead Pollution in Soil, and suffered lead poisoning is all from soil, further
Enhance the accuracy and feasibility of Atrichum undulatum detection Lead Pollution in Soil.Specification of the present invention bryophyte detection environment is dirty
The technical standard of dye has high application value and promotion prospect.
It is of the invention specific steps are as follows：
The cultivation of vegetable material obtains process：
1. preparing spore suspension：The sporinite for acquiring spore growth phase mature Atrichum undulatum plant is several, is put in
In quartzy mortar, after a little liquid nitrogen is added, it is lightly ground to the luxuriant capsule of sporinite and is completely severed, so that built-in spore is shed, add
Enter distilled water repeated flushing mortar, washing lotion is passed through to the stainless steel filtering net that cell inner diameter is 1mm together and is filtered, big portion is filtered
The luxuriant capsule residuum divided, to obtain spore suspension；
2. preparing growth substrate：With volume ratio be 1 ︰ 1 clean sandy soil and plant residue fermentation liquid (with flowers retting
Fertilizer replacement) it is matrix formulations, Atrichum undulatum growth substrate is used as after being sufficiently mixed, which has quality loose, organic
The features such as matter rich content, moisture retention be strong, good permeability, is suitble to bryophyte growth.The growth substrate prepared is laid in
Diameter 35cm, high 6cm polyethylene tray in, stromal thickness 5cm.
3. condition of culture is arranged：Spore suspension is uniformly sprayed in culture substrate, artificial lighting weather culture is placed in
In case, initial incubation temperature setting is 22 DEG C~26 DEG C, and intensity of illumination is set as 2500Lux/10h (before period simulation emergence
The field environment of four Mays), the daily Hogl and nutrient solution 300-400mL/ disk for spraying 1/6 concentration protects soil humidity
It holds 70%~80%；Atrichum undulatum seedling starts to grow after 20~30d, sets 32 DEG C~37 for cultivation temperature at this time
DEG C, intensity of illumination is set as 5000Lux/14h (field environments of six Julys after period simulation emergence)；40 after emergence~
50d enters the spore growth stage, using the plant in the period as the detection material of Lead Pollution in Soil.
4. the design and use of double-layer isolated type soil pick-up dust cover：
Dust guard pollution abatement equipment of the present invention is not having any impact to the growth of Atrichum undulatum, development and physiological status
Under the premise of, the expeditiously contact in Isolated Shield air containing land pollutant with plant, reduction air pollution pair as far as possible
The influence of plant, to promote Atrichum undulatum to the detection effect and quality of Lead Pollution in Soil.The design process of the product
It is with application method：Mainly include the supporting support of a set of similar cone shape, is made of that (Fig. 1 shows this to set small one and large one
Standby longitudal section), solid line indicates that the big bracket of set outside is outrigger 1, and dotted line indicates that the small rack of the inside is internal frame 2；Bracket
Making material can be made of iron wire or other flexible materials, and bracket is built should be simple as far as possible, only by 4 iron wires on circular conical surface
(or other materials) connection top and bottom annulus；Two sets of brackets whole body food fresh keeping membrane (or other are thin and transparent
Material) package, and the part (shown in figure grey area) between two stands bottom is also even sealed with membrane material, as active carbon
Loading end；Inner support lower hollow is not closed, convenient for back-off plant；Hole is respectively kept in the whole body wrap film of inside and outside two stands
Hole, under exit orifice 4 occupies, aperture is slightly larger, and on the residence of inner hole 3, aperture is slightly smaller；The size of two cone supports is depending on actual needs.
In use, placing layer of active carbon layer 5 on connecting the loading end between two stands bottom, then the bracket is integrally buckled in wave
On leaf red crowned crane moss plant.
Dust guard pollution abatement equipment whole design of the present invention is at cone shape, and top area is small, lower part area is big, on the one hand
Reduce the retention area to dust particles leaded in air to the maximum extent, is on the other hand conducive to the product in use process
In stability, be not easy to lodge；Bracket builds the reasons why simple, transparent membrane package and is to try to reduce illumination shelter, both protects
It has demonstrate,proved and air insulated, and has not influenced light transmission efficiency, so that bryophyte be made to be normally carried out photosynthesis；There are holes on interior outer membrane
Hole be it is unimpeded with the gas exchanges of external environment in order to guarantee, it is to hinder that outer fenestra orientation is on the lower side, interior fenestra orientation is on the upper side
Keep off leaded dust particles of the part from the elegant entrance of exit orifice, make its no longer upwardly through interior fenestra enter subintimal space to
It is contacted with plant；The leaded dust particles entered from outer fenestra, substantially can all be held by the bottom between two stands
Active carbon on section is adsorbed.To sum up, the use of the double-layer isolated type soil pick-up dust cover product, can remove sky to the maximum extent
Influence in gas containing land pollutant to Atrichum undulatum, and entire production process is simple, consumptive material is small, low cost, user
Just, reusable, it has no effect to environment, therefore research practice and auxiliary relevant departments development detection work can be largely used to
Make.
When specific detection, the above-mentioned Atrichum undulatum plant cultivated and obtained is taken, is transplanted in soil to be detected, it will be above-mentioned
Designed double-layer isolated type soil pick-up dust cover (having placed active carbon) out, which is buckled, to be placed on Atrichum undulatum plant, to be grown in nothing
Plant in lead contamination matrix is control, observes the variation of its appearance character, and sample in 3~5d, measures and compare plant
Chlorophyll content, soluble protein content and MDA concentration change according to These parameters, and the lead of overall merit soil to be detected is dirty
Dye degree and ecological security.
To further illustrate effect and Atrichum undulatum caused by vegetable material acquisition methods according to the present invention
To the sensivity feature of Lead Pollution in Soil, verified by the way of gradient Lead sweet in soil matrix.With domestic soil
Earth Lead pollution environment quality background value is that reference makes every part of samples-soil by way of manually quantitatively adding lead nitrate solution
In plumbum ion concentration be respectively 35.0mgkg-1、70.0mg·kg-1、140.0mg·kg-1、280.0mg·kg-1, add with zero
The clean soil added is as control；This technology is cultivated respectively obtaining and direct from the shifting of the Atrichum undulatum plant of field acquisition
It plants in the soil matrix of above-mentioned different lead concentrations, is placed in growth cabinet that (air in growth cabinet can be considered clean
Net air can not consider the influence of leaded dust particles)；From the 2nd day after transplanting, the next day observe Atrichum undulatum growth
Reaction, while drawing materials and plant is measured with the chlorophyll content of ethanol extraction method measurement Atrichum undulatum, Coomassie Brilliant Blue
Soluble protein content, thiobarbituricacidα- method measure plant malonaldehyde (MDA) content, are carried out continuously 3 times, by data observation
As a result it is summarized in the following table 1.By data it is found that compared with the blank control soil of no lead contamination, acquisition is cultivated through the present invention
Atrichum undulatum, in low lead content (35.0mgkg-1For level-one soil lead tolerance standard) soil in show aggrieved disease
Shape, physical signs generate significant changes；Extend as Lead sweet concentration increases with the time, plant extent of injury is deepened, aggrieved disease
Shape is further obvious；(the 280.0mgkg when lead concentration is close to three-level soil lead tolerance standard-1), Atrichum undulatum reaches tolerance
Limit value, plant withered death, it can be seen that, Atrichum undulatum is extremely strong to Lead Pollution in Soil sensibility, can be used as detection Pb in Soil
The ideal material of pollution.Simultaneously, it was also found that the Atrichum undulatum plant without the cultivation of the technology of the present invention process is (directly from field ring
Acquire and obtain in border) when making detection material, under lead contamination stress compared with blank control, between indices measurement result
Variation degree reduce, illustrate that the susceptibility of the plant pair Lead Pollution in Soil directly acquired from field decreases.It is former
Because being, the Atrichum undulatum in natural environment produces certain pollution in long-term growth course to field environment
Adaptability, this reduces somewhat its susceptibility to Lead Pollution in Soil, and related spore training through the invention
Plant process eliminates its background adaptation i.e. departing from original growing environment since spore phase (the as seed of the plant)
Property, and the sensivity feature to lead contamination is remained to the maximum extent, therefore the detection material preferably as Lead Pollution in Soil.
1 Atrichum undulatum of table analyzes the sensivity feature of Lead Pollution in Soil
Chlorophyll and soluble protein can be used as the evaluation index of plant growth health status, and content value is higher to illustrate plant
Upgrowth situation is better；Malonaldehyde is the substance that plant generates when by environment stress, and content value is bigger to illustrate hazard of plant journey
It spends higher.By being compared in upper table 1 to the detection of several sensibility indexs, it can be clearly seen that, it is trained by the technology of the present invention
The Atrichum undulatum plant material obtained is planted, the plant of acquisition is substantially better than without cultivation and directly acquired in detection performance
Strain.
For the effect for further verifying dust cover according to the present invention, the mode of field condition verification is taken, by wave
Leaf red crowned crane moss plant is divided into two parts, is cultivated in identical no lead-contaminated soil matrix respectively, and a copy of it is hidden using dust cover
Lid, another is not covered, while being placed on the ground near local one medium-sized thermal power plant's exhausting chimney, is timed to daily
Field observation Atrichum undulatum appearance upgrowth situation and reaction are fetched after placing 4d, after sterile water wash plant, are respectively adopted
Ethanol extraction method measures the chlorophyll content of Atrichum undulatum, Coomassie Brilliant Blue measures plant soluble protein content, stone
The content of lead element, measurement result are as shown in table 2 below in black furnace atomic absorption spectroscopy determination vegetable material.It is surveyed from table
Result is determined as it can be seen that the heavy metal particles in industrial pollution area air have preferably been isolated in developed dust cover, thus celestial to wave leaf
Crane moss plays a protective role；The Atrichum undulatum plant poisoned without using dust cover by air pollutants, chlorophyll contain
Amount, soluble protein content be decreased significantly (two indexs compare respectively using dust cover cover plant has dropped 31.6%
With 34.4%), it is consistent with plant damage symptoms；Most illustrative is that Atrichum undulatum contains the absorption enrichment of lead element
Amount effectively hinders absorption of the plant to Lead in Air after dust cover, and the plant of dust cover is not used, lead
Absorption strength is up to 17.95mgkg-1, it is 28.5 times using dust cover plant.It can be seen that using Atrichum undulatum into
When row Lead Pollution in Soil detects, which has preferable dust-proof soil pick-up effect, can efficiently exclude Lead in Air dirt
Contaminate the interference and influence of object.
Protective effect of 2 dust cover of table to the isolation effect of air lead contamination and its to Atrichum undulatum growth
By analyzing the implementation result of upper table 2 it is found that dust cover according to the present invention has been effectively isolated lead in atmosphere
The influence of equal polluters, preferably protects growing process.
In summary two embodiments are as it can be seen that cultivating the Atrichum undulatum obtained using the present invention has Lead Pollution in Soil
High sensivity feature, double-layer isolated type soil pick-up dust cover can effectively exclude the influence of Lead in Air, and the two cooperates, can
Realize that Atrichum undulatum detects Lead Pollution in Soil high accurancy and precision.
The above is only the preferred embodiments of the present invention, and is not intended to limit the present invention in any form,
Any simple modification made to the above embodiment according to the technical essence of the invention, equivalent variations and modification, belong to
In the range of technical solution of the present invention.
Claims (3)
1. a kind of method for accurately detecting Lead Pollution in Soil degree based on Atrichum undulatum, which is characterized in that this method includes plant
The cultivation acquisition and the dust-proof detection of double-layer isolated type soil pick-up of material；
The cultivation of vegetable material included：
Prepare spore suspension：The sporinite for acquiring spore growth phase mature Atrichum undulatum plant is several, is put in quartz and grinds
It in alms bowl, after a little liquid nitrogen is added, is lightly ground to the luxuriant capsule of sporinite and is completely severed, built-in spore is made to shed, distillation is added
Washing lotion is passed through the stainless steel filtering net that cell inner diameter is 1mm together and is filtered, filters most bud by water repeated flushing mortar
Capsule residuum, to obtain spore suspension；
Prepare culture substrate：It is sufficiently mixed using the clean sandy soil and plant residue fermentation material that volume ratio is 1 ︰ 1 as matrix formulations
Atrichum undulatum growth substrate is used as after conjunction；The growth substrate prepared is laid in the polyethylene tray of diameter 35cm, high 6cm
In, stromal thickness 5cm；
Condition of culture is set：Spore suspension is uniformly sprayed in culture substrate, is placed in artificial lighting weather incubator, mould
The field environment for drawing up Miao Qiansi May starts to grow to Atrichum undulatum seedling；Then six Julys after simulation emergence
Field environment continues to cultivate；40~50d enters the spore growth stage after emergence, using the plant in the period as Lead Pollution in Soil
Detection material；
Double-layer isolated type soil pick-up it is dust-proof detection include：Using the covering of double-layer isolated type soil pick-up dust guard for as Pb in Soil dirt
Contaminate the plant of detection；The double-layer isolated type soil pick-up dust guard is provided with the supporting support of cone shape, by small one and large one
Composition；By 4 iron wire connection top and bottom annulus on circular conical surface；Food fresh keeping membrane is used on two sets of brackets whole body or other are thin
And transparent material package, and the part between two stands bottom is also even sealed with membrane material, the loading end as active carbon；Interior branch
Frame lower hollow is not closed；Hole is respectively kept in the whole body wrap film of inside and outside two stands, under exit orifice occupies, aperture is slightly larger, inner hole
On residence, aperture is slightly smaller；In use, placing layer of active carbon on the loading end of connection two stands bottom, then the bracket is whole
It is buckled on Atrichum undulatum plant.
2. the method according to claim 1 for accurately detecting Lead Pollution in Soil degree based on Atrichum undulatum, which is characterized in that
Plant residue fermentation material is with the replacement of flowers retting fertilizer.
3. the method according to claim 1 for accurately detecting Lead Pollution in Soil degree based on Atrichum undulatum, which is characterized in that
Spore suspension is uniformly sprayed in culture substrate, is placed in artificial lighting weather incubator, initial incubation temperature setting is
22 DEG C~26 DEG C, intensity of illumination is set as 2500Lux/10h, the daily Hogland nutrient solution 300-400mL/ for spraying 1/6 concentration
Disk makes soil humidity be maintained at 70%~80%；Atrichum undulatum seedling starts to grow after 20~30d, at this time by cultivation temperature
32 DEG C~37 DEG C are set as, intensity of illumination is set as 5000Lux/14h；40~50d enters the spore growth stage after emergence, will
Detection material of the plant in the period as Lead Pollution in Soil.
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| CN109541149B (en) * | 2018-12-29 | 2023-06-06 | 广东工业大学 | Biological type atmospheric heavy metal pollution detection and discrimination device and application |
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