CN106770238A - The detector of beta-agonist class in a kind of meat products - Google Patents
The detector of beta-agonist class in a kind of meat products Download PDFInfo
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- CN106770238A CN106770238A CN201611113596.1A CN201611113596A CN106770238A CN 106770238 A CN106770238 A CN 106770238A CN 201611113596 A CN201611113596 A CN 201611113596A CN 106770238 A CN106770238 A CN 106770238A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
Abstract
The invention discloses a kind of detector of beta-agonist class in meat products, including base, the upper surface of the base is fixedly connected with cantilever, one end of the cantilever is fixedly connected with spring clip, material storing cup is clamped with the spring clip, the lower surface of the material storing cup is fixedly connected with siphunculus, stop valve is fixedly connected with the siphunculus, the bottom of the siphunculus is fixedly connected with three-way pipe, and the upper surface of the base is also fixedly connected with reagent rack and spectrophotometer.Detector by providing beta-agonist class in a kind of meat products of the invention, sample does not need cumbersome pre-treatment, and instrument price is relatively inexpensive, and the detector is simple to operate, detection cycle is shorter, effective monitoring can be carried out to the meat products of a large amount of circulations of in the market, the meat products containing clenbuterol hydrochloride can be carried out by first screening slightly by the detector, be adapted to promote the use of solve the present situation that meat products in the market contains β classes excitant and is difficult to detection.
Description
Technical field
The present invention relates to food inspection instrument technical field, the detector of beta-stimulants class in specially a kind of meat products.
Background technology
Excitant often stops at sporting venue in people's eye, and in its tangible life, excitant is also of common occurrence, and I
Meat used in daily life be just possible to carry excitant, the clenbuterol hydrochloride that such as we often hear, then be a kind of β
Class excitant, its entitled clenobuterol hydrochloride.
Clenobuterol hydrochloride is β 2 receptor agonist, belongs to adrenomimetic drug class medicine, trade name " clenbuterol hydrochloride " or " gram
Breathe heavily element ", the beginning of the eighties, a company of the U.S. has been surprisingly found that, Clenizole Hydrochloride can be obviously promoted growth of animal, and increase lean meat
Rate.It can change the metabolic pathway in animal body, promote the synthesis of protein in muscle, particularly skeletal muscle, suppress fat
Synthesis, so as to accelerate the speed of growth, lean meat relative increase improves carcass quality.This new discovery is used by some countries quickly
After with the addition of Clenizole Hydrochloride in aquaculture, feed, the growth of animals or poultry such as pig speed, feed conversion rate and trunk can be made thin
Meat rate improves more than 10%, so trade name of the Clenizole Hydrochloride when being sold as feed addictive is also called " clenbuterol hydrochloride "
" many elements of meat " etc..
Clenobuterol hydrochloride can make the beta receptor on skeletal muscle slow-twitch fiber, be allowed to accelerate to shrink, and showing as hand can not hold
Firmly, difficult on foot, neck surface bone amyostasia, especially sympathetic hyperfunction person are more easy to be occurred, can during day receptor agonism
Suppress the propagation of lymphocyte, suppress the formation of lymphokine and antibody, suppress the platelet activation for inducing；Clenobuterol hydrochloride
Blood glucose rise can be made, insulin secretion increases, and causes apocleisis；Clenobuterol hydrochloride can Na/K+ on exciting skeletal muscle cell membrane/
ATP enzyme, promotes K+ to enter cell, and K+ concentration declines in blood, induces arrhythmia cordis.Additionally, beta 2 receptor excitation energy causes blood breast
Acid, pyruvic acid are raised, and may occur in which ketoboidies, cause metabolic disorder.It is bad anti-that these pharmacological actions are that clenobuterol hydrochloride causes
The reason for answering.
Because clenbuterol hydrochloride is very harmful, so clearly prohibitted the use of in poultry farming, but in order to avoid these make
Come into the market with the poultry of clenbuterol hydrochloride, so essential to detection, China by HPLC it is legal be half authenticity method,
China by GCMS it is legal be authenticity method, above method detection process is cumbersome, detection time is long and need instrument it is valuable
And it is difficult to operate, it is difficult to be embodied in meat supervision, although and ELISA kit that foreign countries are developed can be fast
The residual of fast efficient detection clenbuterol hydrochloride, but its price expense is high, does not detect the expense of a pork in 1200-1400 units
Between, it is difficult to promote the use of.
The content of the invention
（One）The technical problem of solution
In view of the shortcomings of the prior art, the invention provides a kind of detector of beta-stimulants class in meat products, meat system is solved
Without the problem of a kind of efficient, cheap detection means in the detection management of product.
（Two）Technical scheme
To achieve the above object, the present invention provides following technical scheme：The detector of beta-stimulants class in a kind of meat products, including
Base, the upper surface of the base is fixedly connected with cantilever, and one end of the cantilever is fixedly connected with spring clip, the spring clip
Material storing cup is inside clamped with, the lower surface of the material storing cup is fixedly connected with siphunculus, stop valve, institute are fixedly connected with the siphunculus
The bottom for stating siphunculus is fixedly connected with three-way pipe, and the upper surface of the base is also fixedly connected with reagent rack and spectrophotometer,
Two test tubes are plugged with the reagent rack, swivel mount is fixedly connected with the reagent rack, drop is plugged with the swivel mount
Pipe, the cuvette of three ports of the three-way pipe respectively with two test tubes and spectrophotometer is connected.
Preferably, the spectrophotometric is calculated as ultraviolet specrophotometer.
Preferably, the quantity of the dropper is five, and the bottom of dropper is provided with rotary glass plug, and five droppers
Storage respectively has watery hydrochloric acid, 0.1mol/L sodium nitrite solutions, alkaline betanaphthol test solution, nitric acid and silver nitrate.
Preferably, the material storing cup internal memory is placed with 150-155ml clear water.
Preferably, it is additionally provided with screen cloth in the siphunculus.
Preferably, three outlet calibers of the three-way pipe are equal.
Preferably, its detecting step is as follows：
A. meat material to be detected is cut into small pieces to be put into material storing cup, static at least 5 minutes so that the hydrochloric acid gram included in meat material
Lun Teluo is completely dissolved in clear water；
B.5-10 after minute, stop valve is opened so that the solution in material storing cup has outflow in siphunculus, and is shunted via three-way pipe,
Solution after shunting is injected separately into the cuvette of two test tubes and spectrophotometer；
C. by the wavelength of spectrophotometric determination solution, see whether solution obtains maximum suction when wavelength is 243nm and 296nm
Receive；
D. rotation swivel mount makes the dropper containing watery hydrochloric acid be located at a surface for test tube, opens glass stopper, is dripped in test tube
Plus the watery hydrochloric acid of 1ml, static 2-3 minutes waits its dissolving, and swivel mount is rotated again, makes to contain 0.1mol/L sodium nitrite solutions
Dropper be located at this test tube surface, open glass stopper, be added dropwise 2-5 drop 0.1mol/L sodium nitrite solutions, again rotation revolve
Pivoted frame, makes the dropper containing alkaline betanaphthol test solution be located at the surface of test tube, opens glass stopper, and 2-5 drops alkalescence β-naphthalene is added dropwise
Phenol test solution, observes whether invisible spectro solution is generated by the orange precipitation to scarlet, if generation scarlet precipitation, shows virtue
The identification of fragrant first amine；
E. rotation swivel mount makes the dropper containing salpeter solution be located at the surface of another test tube, glass stopper is opened, to test tube
Interior dropwise addition 2-3 drops nitric acid is acidified to invisible spectro solution, excludes the interference of other ions, then rotates swivel mount, make containing
The dropper of silver nitrate solution is located at the surface of test tube, opens glass stopper, instills 2-4 drop nitric acid argentum reagents, is in observation test tube
No generation white precipitate, shows the identification of chloride if white precipitate is produced；
F. by above-mentioned Analysis of test results, if meat material soak had both shown fragrant first amine identification, chloride is shown again
Identification, and solution wavelength wavelength be 243nm and 296nm when obtain absorption maximum in the case of, then contain salt in meat material
Clenbuterol, if conversely, in three testing results, there is any one not to be inconsistent, then not containing clenobuterol hydrochloride in meat material.
（Three）Beneficial effect
The invention provides a kind of detector of beta-stimulants class in meat products.Possesses following beneficial effect：
Detector by providing beta-stimulants class in a kind of meat products of the invention, in detection process, sample need not be cumbersome
Pre-treatment, and instrument price is relatively inexpensive, and required classes of agents is also that in the market circulates high common reagent, and price is just
Preferably, and the detector is simple to operate, detection cycle is shorter, can carry out effective prison to the meat products of a large amount of circulations of in the market
Control, first screening slightly can be carried out by the detector by the meat products containing clenbuterol hydrochloride, be adapted to promote the use of solve at present
In the market meat products contains β classes excitant and is difficult to the present situation for detecting.
Brief description of the drawings
Fig. 1 is schematic structural view of the invention.
In figure：1 base, 2 cantilevers, 3 spring clips, 4 material storing cups, 5 siphunculus, 6 stop valves, 7 three-way pipes, 8 reagent racks, 9 light splitting
Photometer, 10 test tubes, 11 swivel mounts, 12 droppers.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
As shown in figure 1, the present invention provides a kind of technical scheme：The detector of beta-stimulants class in a kind of meat products, including
Base 1, the upper surface of base 1 is fixedly connected with cantilever 2, and one end of cantilever 2 is fixedly connected with spring clip 3, by setting spring
Folder 3, allows material storing cup 4 freely to disassemble, and material storing cup 4 is clamped with spring clip 3, and the internal memory of material storing cup 4 is placed with 150-155ml
Clear water, because clenobuterol hydrochloride is water-soluble, meat material is immersed in clear water, can cause that clenobuterol hydrochloride is dissolved in water
In, so as to be extracted, the lower surface of material storing cup 4 is fixedly connected with and screen cloth is additionally provided with siphunculus 5, siphunculus 5, sets screen cloth
Stop that meat bits flow out from siphunculus 5, block three-way pipe 7, stop valve 6 is fixedly connected with siphunculus 5, stop valve 6 is set, so that
The liquid in material storing cup 4 can be controlled to flow, the bottom of siphunculus 5 is fixedly connected with three-way pipe 7, three outlets of three-way pipe 7
Footpath is equal, by setting three-way pipe 7, soak is evenly distributed in two test tubes 10 and spectrophotometer, so that
So that detection is simple efficiently, the upper surface of base 1 is also fixedly connected with reagent rack 8 and spectrophotometer 9, grafting in reagent rack 8
There are two test tubes 10, swivel mount 11 is fixedly connected with reagent rack 8, by setting swivel mount 11 so that dropper 12 can pass through
Rotation is easy to use so that simple to operate directly just to test tube 10, and dropper 12 is plugged with swivel mount 11, dropper 12
Quantity is five, and the bottom of dropper 12 is provided with rotary glass plug, and in five droppers 12 respectively storage have watery hydrochloric acid,
0.1mol/L sodium nitrite solutions, alkaline betanaphthol test solution, nitric acid and silver nitrate, three ports of three-way pipe 7 respectively with two
Test tube 10 is connected with the cuvette of spectrophotometer 9, and spectrophotometer 9 is ultraviolet specrophotometer.
When using, its detecting step is as follows：
A. meat material to be detected is cut into small pieces to be put into material storing cup 4, static at least 5 minutes so that the hydrochloric acid included in meat material
Clenbuterol is completely dissolved in clear water；
B.5-10 after minute, stop valve 6 is opened so that the solution in material storing cup 4 has an outflow in siphunculus 5, and via 7 points of three-way pipe
Stream, the solution after shunting is injected separately into the cuvette of two test tubes 10 and spectrophotometer 9；
C. the wavelength of solution is determined by spectrophotometer 9, sees whether solution obtains maximum when wavelength is 243nm and 296nm
Absorb；
D. rotation swivel mount 11 makes the dropper 12 containing watery hydrochloric acid be located at a surface for test tube 10, glass stopper is opened, to examination
The watery hydrochloric acid of 1ml is added dropwise in pipe 10, static 2-3 minutes waits its dissolving, and swivel mount 11 is rotated again, makes sub- containing 0.1mol/L
The dropper 12 of sodium nitrate solution is located at the surface of this test tube 10, opens glass stopper, 2-5 drop 0.1mol/L natrium nitrosums is added dropwise molten
Liquid, rotates swivel mount 11 again, the dropper 12 containing alkaline betanaphthol test solution is located at the surface of test tube 10, opens glass
Plug, is added dropwise 2-5 drops alkalescence betanaphthol test solution, and whether the solution observed in test tube 10 is generated by the orange precipitation to scarlet, such as
Fruit generation scarlet precipitation, then show the identification of fragrant first amine；
E. rotation swivel mount 11 makes the dropper 12 containing salpeter solution be located at the surface of another test tube 10, opens glass stopper,
The solution in test tube 10 is acidified to 2-3 drops nitric acid is added dropwise in test tube 10, excludes the interference of other ions, then rotation
Frame 11, makes the dropper 12 containing silver nitrate solution be located at the surface of test tube 10, opens glass stopper, instills the examination of 2-4 drops silver nitrate
Whether agent, white precipitate is produced in observation test tube 10, and the identification of chloride is shown if white precipitate is produced；
F. by above-mentioned Analysis of test results, if meat material soak had both shown fragrant first amine identification, chloride is shown again
Identification, and solution wavelength wavelength be 243nm and 296nm when obtain absorption maximum in the case of, then contain salt in meat material
Clenbuterol, if conversely, in three testing results, there is any one not to be inconsistent, then not containing clenobuterol hydrochloride in meat material.
Can to sum up obtain, the detector by providing beta-stimulants class in a kind of meat products of the invention, in detection process, sample
Product do not need cumbersome pre-treatment, and instrument price is relatively inexpensive, and required classes of agents is also that in the market circulates high leading to
It is cheap with reagent, and the detector is simple to operate, and detection cycle is shorter, can be to the meat system of a large amount of circulations of in the market
Product are effectively monitored, and the meat products containing clenbuterol hydrochloride can be carried out into first screening slightly by the detector, are adapted to promote
Using solve meat products in the market contain β classes excitant and be difficult to detection present situation.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation make a distinction with another entity or operation, and not necessarily require or imply these entities or deposited between operating
In any this actual relation or order.And, term " including ", "comprising" or its any other variant be intended to
Nonexcludability is included, so that process, method, article or equipment including a series of key elements not only will including those
Element, but also other key elements including being not expressly set out, or also include being this process, method, article or equipment
Intrinsic key element.In the absence of more restrictions, the key element limited by sentence "including a ...", it is not excluded that
Also there is other identical element in process, method, article or equipment including the key element.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding can carry out various changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention
And modification, the scope of the present invention be defined by the appended.
Claims (7)
1. in a kind of meat products beta-stimulants class detector, including base（1）, it is characterised in that：The base（1）Upper table
Face is fixedly connected with cantilever（2）, the cantilever（2）One end be fixedly connected with spring clip（3）, the spring clip（3）Interior clamping
There is material storing cup（4）, the material storing cup（4）Lower surface be fixedly connected with siphunculus（5）, the siphunculus（5）On be fixedly connected with cut
Only valve（6）, the siphunculus（5）Bottom be fixedly connected with three-way pipe（7）, the base（1）Upper surface be also fixedly connected with
Reagent rack（8）And spectrophotometer（9）, the reagent rack（8）On be plugged with two test tubes（10）, the reagent rack（8）It is upper solid
Surely it is connected with swivel mount（11）, the swivel mount（11）On be plugged with dropper（12）, the three-way pipe（7）Three ports difference
With two test tubes（10）And spectrophotometer（9）Cuvette be connected.
2. in a kind of meat products according to claim 1 beta-stimulants class detector, it is characterised in that：The light splitting light
Degree meter（9）It is ultraviolet specrophotometer.
3. in a kind of meat products according to claim 1 beta-stimulants class detector, it is characterised in that：The dropper
（12）Quantity be five, and dropper（12）Bottom be provided with rotary glass plug, and five droppers（12）Inside storage has respectively
Watery hydrochloric acid, 0.1mol/L sodium nitrite solutions, alkaline betanaphthol test solution, nitric acid and silver nitrate.
4. in a kind of meat products according to claim 1 beta-stimulants class detector, it is characterised in that：The material storing cup
（4）Internal memory is placed with 150-155ml clear water.
5. in a kind of meat products according to claim 1 beta-stimulants class detector, it is characterised in that：The siphunculus
（5）Inside it is additionally provided with screen cloth.
6. in a kind of meat products according to claim 1 beta-stimulants class detector, it is characterised in that：The three-way pipe
（7）Three outlet calibers it is equal.
7. in a kind of meat products according to claim 1 beta-stimulants class detector, it is characterised in that its detecting step
It is as follows：
A. meat material to be detected is cut into small pieces and is put into material storing cup（4）It is interior, static at least 5 minutes so that the salt included in meat material
Clenbuterol is completely dissolved in clear water；
B.5-10 after minute, stop valve is opened（6）So that material storing cup（4）Interior solution has siphunculus（5）Interior outflow, and via three
Siphunculus（7）Shunting, the solution after shunting is injected separately into two test tubes（10）And spectrophotometer（9）Cuvette in；
C. spectrophotometer is passed through（9）The wavelength of solution is determined, sees whether solution obtains most when wavelength is 243nm and 296nm
It is big to absorb；
D. swivel mount is rotated（11）Make the dropper containing watery hydrochloric acid（12）Positioned at a test tube（10）Surface, open glass
Plug, to test tube（10）The watery hydrochloric acid of interior dropwise addition 1ml, static 2-3 minutes waits its dissolving, and swivel mount is rotated again（11）, make to contain
There is the dropper of 0.1mol/L sodium nitrite solutions（12）Positioned at this test tube（10）Surface, open glass stopper, be added dropwise 2-5 drops
0.1mol/L sodium nitrite solutions, rotate swivel mount again（11）, make the dropper containing alkaline betanaphthol test solution（12）Positioned at examination
Pipe（10）Surface, open glass stopper, 2-5 drops alkalescence betanaphthol test solution, observation test tube is added dropwise（10）Whether interior solution gives birth to
Into by the orange precipitation to scarlet, if generation scarlet precipitation, shows the identification of fragrant first amine；
E. swivel mount is rotated（11）Make the dropper containing salpeter solution（12）Positioned at another test tube（10）Surface, open glass
Glass plug, to test tube（10）Interior dropwise addition 2-3 drop nitric acid is to test tube（10）Interior solution is acidified, and excludes the interference of other ions,
Swivel mount is rotated again（11）, make the dropper containing silver nitrate solution（12）Positioned at test tube（10）Surface, open glass stopper, drop
Enter 2-4 drop nitric acid argentum reagents, observe test tube（10）Inside whether white precipitate is produced, chloride is shown if white precipitate is produced
Identification；
F. by above-mentioned Analysis of test results, if meat material soak had both shown fragrant first amine identification, chloride is shown again
Identification, and solution wavelength wavelength be 243nm and 296nm when obtain absorption maximum in the case of, then contain salt in meat material
Clenbuterol, if conversely, in three testing results, there is any one not to be inconsistent, then not containing clenobuterol hydrochloride in meat material.
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| CN201611113596.1A CN106770238A (en) | 2016-12-07 | 2016-12-07 | The detector of beta-agonist class in a kind of meat products |
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Cited By (1)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770995A (en) * | 2016-11-30 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | The detection kit of beta-agonist in a kind of food |
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Application publication date: 20170531 |