CN205333588U

CN205333588U - Remain measurement of quantities system to salbutamol among animal -derived food

Info

Publication number: CN205333588U
Application number: CN201620083426.2U
Authority: CN
Inventors: 常锋
Original assignee: Gansu Guoxin Runda Analysis Testing Center
Current assignee: Gansu Guoxin Runda Analysis Testing Center
Priority date: 2016-01-27
Filing date: 2016-01-27
Publication date: 2016-06-22
Anticipated expiration: 2026-01-27

Abstract

The utility model discloses a remain measurement of quantities system to salbutamol among animal -derived food, it includes sample thief etc, the sample thief links to each other with first centrifuging tube, first centrifuging tube is located temperature controller, temperature controller is located the vibrator, the vibrator is located supersonic generator, additive container and first centrifuging tube upper end links to each other, the second centrifuging tube is located electronic oscillator, the lower extreme of second centrifuging tube links to each other with the upper end of extraction column, the eluate container, nitrogen gas tank with on the extraction column links to each other, the ration container is located the extraction column below, the filter is located quantitative container below, the liquid chromatogram - mass spectrograph, the feed liquor pipe all is located the filter below, organic filter membrane is located the filter downside, ration ring both ends link to each other with feed liquor pipe and chromatographic column respectively, the chromatographic column other end links to each other with the mass spectrometric detection ware. The utility model is simple in operation, it is convenient to calculate, conveniently atomizes in the measurement and be dry, the low price.

Description

To the mensuration system of salbutamolum residue amount in animal-derived food

Technical field

This utility model relates to a kind of mensuration system, particularly relates to a kind of external standard method to the mensuration system of salbutamolum residue amount in animal-derived food。

Background technology

Its dosage form of albuterol has tablet, capsule, aerosol and injection etc., and albuterol is selectivity beta 2 receptor agonist, can effectively suppress histamine etc. to cause the release of anaphylaxis material, it is prevented that bronchospasm。Suitable in diseases such as bronchial asthma, asthmatic bronchitis, bronchospasm, emphysema。Modern Carnis Sus domestica cultivation is used as clenbuterol hydrochloride, improves the lean meat yield of live pig。Within 2002, begin, be listed in cultivation industry forbidden drug, must not add in livestock and poultry cultivation。Existing to the mensuration system complicated operation of salbutamolum residue amount in animal-derived food, calculate inconvenience, measurement is inconvenient to carry out being atomized and drying, price is high。

Summary of the invention

Technical problem to be solved in the utility model is to provide that a kind of it is simple to operate, convenience of calculation to the mensuration system of salbutamolum residue amount in animal-derived food, conveniently carries out being atomized and drying in measurement, cheap。

This utility model solves above-mentioned technical problem by following technical proposals: a kind of to the mensuration system of salbutamolum residue amount in animal-derived food, it is characterised in that it includes sampler, first centrifuge tube, temperature controller, supersonic generator, additive container, vibrator, electric agitator, second centrifuge tube, extraction column, eluate container, nitrogen box, dosing container, filter, organic filter membrane, feed tube, quantitative loop, chromatographic column, liquid chromatograph-mass spectrometer, mass detector, sampler and the first centrifuge tube are connected, first centrifuge tube is positioned at temperature controller, temperature controller is positioned on vibrator, and vibrator is positioned at supersonic generator, additive container and the first centrifuge tube upper end and is connected, first centrifuge tube upper end is connected with the second centrifuge tube upper end, second centrifuge tube is positioned at electric agitator, and the lower end of the second centrifuge tube is connected with the upper end of extraction column, eluate container, nitrogen box is all connected with extraction column upper end, and dosing container is positioned at below extraction column, and filter is positioned at below dosing container, liquid chromatograph-mass spectrometer, feed tube is all located at below filter, and organic filter membrane is positioned on the downside of filter, and quantitative loop two ends are connected with feed tube and chromatographic column respectively, and the chromatographic column other end is connected with mass detector。

Preferably, described chromatographic column is C18 type capillary chromatographic column。

Preferably, described extraction column is mixed type cation exchange solid-phase extraction column。

Preferably, described first centrifuge tube and the second centrifuge tube are all polypropylene centrifuge tube。

Positive progressive effect of the present utility model is in that: this utility model is suitable to substantial amounts of analysis sample, conveniently carries out being atomized and drying in measurement, simple to operate, convenience of calculation, does not need to measure correction factor, is suitable to automatically analyze, and is easily achieved, cheap。

Accompanying drawing explanation

Fig. 1 is structural representation of the present utility model。

Detailed description of the invention

This utility model preferred embodiment is provided, to describe the technical solution of the utility model in detail below in conjunction with accompanying drawing。

As it is shown in figure 1, the mensuration system of salbutamolum residue amount in animal-derived food is included sampler 1 by this utility model, first centrifuge tube 2, temperature controller 3, supersonic generator 4, additive container 5, vibrator 6, electric agitator 7, second centrifuge tube 8, extraction column 9, eluate container 10, nitrogen box 11, dosing container 12, filter 13, organic filter membrane 14, feed tube 15, quantitative loop 16, chromatographic column 17, liquid chromatograph-mass spectrometer 18, mass detector 19, sampler 1 is connected with the first centrifuge tube 2, first centrifuge tube 2 is positioned at temperature controller 3, temperature controller 3 is positioned on vibrator 6, and vibrator 6 is positioned at supersonic generator 4, and additive container 5 is connected with the first centrifuge tube 2 upper end, first centrifuge tube 2 upper end is connected with the second centrifuge tube 8 upper end, second centrifuge tube 8 is positioned at electric agitator 7, and the lower end of the second centrifuge tube 8 is connected with the upper end of extraction column 9, eluate container 10, nitrogen box 11 is all connected with extraction column 9 upper end, and dosing container 12 is positioned at below extraction column 9, and filter 13 is positioned at below dosing container 12, liquid chromatograph-mass spectrometer 18, feed tube 15 is all located at below filter 13, and organic filter membrane 14 is positioned on the downside of filter 13, and quantitative loop 16 two ends are connected with feed tube 15 and chromatographic column 17 respectively, and chromatographic column 17 other end is connected with mass detector 19。

Extraction column can exchange solid-phase extraction column for mixed type cation, and alkali compounds is had high selectivity and sensitivity by mixed type cation exchange solid-phase extraction column。

First centrifuge tube and the second centrifuge tube can be polypropylene centrifuge tube, so prevent from dissolving with other materials, improve stability, reduce cost。

Chromatographic column can be C18 type capillary chromatographic column, improves precision so further and reduces cost。

Operation principle of the present utility model is as follows: accurately weigh about 5g sample from sampler, it is placed in polypropylene centrifuge tube, add 2mol/L ammonium acetate buffer solution 20 milliliters, ultrasonic 20min in the middle of ice-water bath, is subsequently adding β-glucal glycosides enzyme that 40 μ L enzyme activities are 111000U/mL, and fully vibrate 20h on the vibrator of 37 DEG C, 5000r/min is centrifuged 10min, supernatant pours new polypropylene centrifuge tube into, is simultaneously introduced 20ml normal hexane, vibration mixing。Make normal hexane and aqueous phase layering by centrifugal, draw lower floor's aqueous phase with syringe, do process to be clean。The aqueous phase of absorption is put in the mixed type cation exchange solid-phase extraction column activated, make its naturally lower in atmospheric conditions, then respectively with the 0.1mol/L hydrochloric acid of 3ml, 3ml water, 3ml50% methanol, normal hexane drip washing extraction column, finally carry out eluting with eluent and collect, at 50 DEG C, nitrogen dries up, 1ml mobile phase constant volume, after finally crossing organic filter membrane of 0.22 μm, for liquid chromatograph-mass spectrometer。Feed tube draws the good liquid of 10ml pre-treatment, enters in quantitative loop, mobile phase ratio respectively 80% trifluoroacetic acid aqueous solution and 20% 0.2% formic acid water, through C18 type capillary chromatographic column, column temperature 35 DEG C, be easily separated when flow velocity 0.2ml/min。Material after chromatographic column separates enters into mass detector, Mass Spectrometry Conditions: ionization mode sprays ionization for cation, multiple-reaction monitoring, ionization mode is ESI, collision gas is argon, atomization gas and dry gas are the nitrogen in nitrogen box, and ion source temperature is 250 DEG C, and capillary voltage is 4700V。Nitrogen box is used for providing nitrogen, conveniently carries out being atomized and drying in measurement, dry abundant, and anti-oxidation etc.。Temperature controller makes the first centrifuge tube etc. reach the temperature specified, and additive container has additive, and it is centrifugal that electric agitator carries out vibration for the second centrifuge tube, and extraction column is used for purifying and separating Ractopamine。Eluate container has eluent。Dosing container makes material be maintained in a certain amount of container, it is possible to need not measure capacity, easy to operate。This utility model realizes repeatedly being centrifuged, and can be completely separated out by albuterol, easy to operate。

Particular embodiments described above; to of the present utility model solving the technical problem that, technical scheme and beneficial effect further described; it is it should be understood that; the foregoing is only specific embodiment of the utility model; it is not limited to this utility model; all within spirit of the present utility model and principle, any amendment of making, equivalent replacement, improvement etc., should be included within protection domain of the present utility model。

Claims

1. one kind to the mensuration system of salbutamolum residue amount in animal-derived food, it is characterised in that it includes sampler, first centrifuge tube, temperature controller, supersonic generator, additive container, vibrator, electric agitator, second centrifuge tube, extraction column, eluate container, nitrogen box, dosing container, filter, organic filter membrane, feed tube, quantitative loop, chromatographic column, liquid chromatograph-mass spectrometer, mass detector, sampler and the first centrifuge tube are connected, first centrifuge tube is positioned at temperature controller, temperature controller is positioned on vibrator, and vibrator is positioned at supersonic generator, additive container and the first centrifuge tube upper end and is connected, first centrifuge tube upper end is connected with the second centrifuge tube upper end, second centrifuge tube is positioned at electric agitator, and the lower end of the second centrifuge tube is connected with the upper end of extraction column, eluate container, nitrogen box is all connected with extraction column upper end, and dosing container is positioned at below extraction column, and filter is positioned at below dosing container, liquid chromatograph-mass spectrometer, feed tube is all located at below filter, and organic filter membrane is positioned on the downside of filter, and quantitative loop two ends are connected with feed tube and chromatographic column respectively, and the chromatographic column other end is connected with mass detector。

2. as claimed in claim 1 to the mensuration system of salbutamolum residue amount in animal-derived food, it is characterised in that described chromatographic column is C18 type capillary chromatographic column。

3. as claimed in claim 1 to the mensuration system of salbutamolum residue amount in animal-derived food, it is characterised in that described extraction column is mixed type cation exchange solid-phase extraction column。

4. as claimed in claim 1 to the mensuration system of salbutamolum residue amount in animal-derived food, it is characterised in that described first centrifuge tube and the second centrifuge tube are all polypropylene centrifuge tube。