CN205374398U - Remain measurement of quantities system to clenbuterol among animal -derived food - Google Patents
Remain measurement of quantities system to clenbuterol among animal -derived food Download PDFInfo
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- CN205374398U CN205374398U CN201620083391.2U CN201620083391U CN205374398U CN 205374398 U CN205374398 U CN 205374398U CN 201620083391 U CN201620083391 U CN 201620083391U CN 205374398 U CN205374398 U CN 205374398U
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- centrifuge tube
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- chromatographic column
- clenbuterol
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Abstract
The utility model discloses a remain measurement of quantities system to clenbuterol among animal -derived food, it includes sampler etc, the sampler links to each other with first centrifuging tube, the upper end of first centrifuging tube links to each other with the upper end of second centrifuging tube, the second centrifuging tube is located the low temperature temperature -controlled instrument, the low temperature temperature -controlled instrument is located ultrasonic generrator, the second centrifuging tube is fixed in in the centrifugal oscillator, the lower extreme of second centrifuging tube links to each other with the upper end of extraction column, the extract container all with on the extraction column links to each other with the eluate container, the extraction column lower extreme links to each other with organic filter membrane upside, the injector is located organic filter membrane downside, the injector other end links to each other with the one end of ration ring, the one end of ration ring links to each other with the one end of chromatographic column, the other end of chromatographic column links to each other with the liquid chromatogram - mass spectrometry appearance, the chromatographic column, the liquid chromatogram - mass spectrometry appearance all is connected with the mass spectrometric detection ware. The utility model is simple in operation, clenbuterol among the messenger animal -derived food remains and thoroughly isolates, easily realizes the low price.
Description
Technical field
This utility model relates to a kind of mensuration system, particularly relates to a kind of to the mensuration system of Determination of Clenbuterol Residues in animal-derived food.
Background technology
Clenbuterol is clinically as the medicine for the treatment of asthma and bronchospasm.In recent years, illegally made an addition in animal feed to improve the lean meat percentage of animal, cause Clenbuterol to remain in a large number in animal muscle and internal organs, after people eats this kind of animal foodstuff, liver, kidney and nervous system injury can be caused.European and American countries forbids eating Clenbuterol as feed additive already, and the Ministry of Agriculture of China also prohibited in animal feed interpolation Clenbuterol with dispatch in 1997, but has minority raiser still to disobey orders use Clenbuterol, and the health of crowd in serious threat.
Liquid chromatography mass combined instrument (liquidChromatographMassSpectrometer), is called for short LC-MS, is the high end instrument in Analysis of Organic Substances market.Organic components in Organic substance testing sample can effectively be separated by liquid chromatograph (LC), and mass spectrum (MS) to the analysis one by one of Organic substance separately, can obtain organic matter molecular mass, structure in some cases and the information of the concentration of quantitative analysis.It is very succinct that powerful electron spray ionisation technology creates LC-MS mass spectrum, and later data processes simple feature.LC-MS is Analysis of Organic Substances laboratory, medicine, food test room, the requisite analytical tool of department such as production process control, quality inspection.
The mensuration system complicated operation of Determination of Clenbuterol Residues in existing animal-derived food, calculates inconvenience, need to measure correction factor, be unsuitable for automatically analyzing, and be difficult to, and price is high.
Summary of the invention
Technical problem to be solved in the utility model is to provide a kind of to the mensuration system of Determination of Clenbuterol Residues in animal-derived food, it is simple to operate, makes the residual of kelengtelu in animal-derived food be completely separated out, is suitable to automatically analyze, and be easily achieved, cheap.
nullThis utility model solves above-mentioned technical problem by following technical proposals: a kind of to the mensuration system of Determination of Clenbuterol Residues in animal-derived food，It is characterized in that，It includes sampler、First centrifuge tube、Low temperature temperature control instrument、Ultrasonic generator、Second centrifuge tube、Centrifugal agitator、Extraction column、Extract container、Eluate container、Organic filter membrane、Injector、Quantitative loop、Chromatographic column、Liquid chromatograph-mass spectrometer、Mass detector，Sampler and the first centrifuge tube are connected，The upper end of the first centrifuge tube is connected with the upper end of the second centrifuge tube，Second centrifuge tube is positioned at low temperature temperature control instrument，Low temperature temperature control instrument is positioned at ultrasonic generator，Second centrifuge tube is fixed in centrifugal agitator，The lower end of the second centrifuge tube is connected with the upper end of extraction column，Extract container is all connected with extraction column upper end with eluate container，Extraction column lower end is connected with on the upside of organic filter membrane，Injector is positioned on the downside of organic filter membrane，The injector other end is connected with one end of quantitative loop，One end of quantitative loop is connected with one end of chromatographic column，The other end of chromatographic column is connected with liquid chromatograph-mass spectrometer，Chromatographic column、Liquid chromatograph-mass spectrometer is all connected with mass detector.
Preferably, described chromatographic column is C18 type capillary chromatographic column.
Preferably, described first centrifuge tube and the second centrifuge tube are all polypropylene centrifuge tube.
Positive progressive effect of the present utility model is in that: this utility model is suitable to substantial amounts of analysis sample, it is simple to operate, improve the accuracy measured and detection speed, convenience of calculation, do not need to measure correction factor, make the residual of kelengtelu in animal-derived food be completely separated out, be suitable to automatically analyze, and be easily achieved, cheap.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Detailed description of the invention
This utility model preferred embodiment is provided, to describe the technical solution of the utility model in detail below in conjunction with accompanying drawing.
nullAs shown in Figure 1，The mensuration system of Determination of Clenbuterol Residues in animal-derived food is included sampler 1 by this utility model、First centrifuge tube 2、Low temperature temperature control instrument 3、Ultrasonic generator 4、Second centrifuge tube 5、Centrifugal agitator 6、Extraction column 7、Extract container 8、Eluate container 9、Organic filter membrane 10、Injector 11、Quantitative loop 12、Chromatographic column 13、Liquid chromatograph-mass spectrometer (liquidChromatographMassSpectrometer)，It is called for short LC-MS) 14、Mass detector 15，Sampler 1 is connected with the first centrifuge tube 2，The upper end of the first centrifuge tube 2 is connected with the upper end of the second centrifuge tube 5，Second centrifuge tube 2 is positioned at low temperature temperature control instrument 3，Low temperature temperature control instrument 3 is positioned at ultrasonic generator 4，Second centrifuge tube 5 is fixed in centrifugal agitator 6，The lower end of the second centrifuge tube 5 is connected with the upper end of extraction column 7，Extract container 8 is all connected with extraction column 7 upper end with eluate container 9，Extraction column 7 lower end is connected with on the upside of organic filter membrane 10，Injector 11 is positioned on the downside of organic filter membrane 10，Injector 11 other end is connected with one end of quantitative loop 12，One end of quantitative loop 12 is connected with one end of chromatographic column 13，The other end of chromatographic column 13 is connected with liquid chromatograph-mass spectrometer 14，Chromatographic column 13、Liquid chromatograph-mass spectrometer 14 is all connected with mass detector 15.
First centrifuge tube and the second centrifuge tube can be polypropylene centrifuge tube, so prevent from dissolving with other materials, improve stability, reduce cost.
Chromatographic column can be C18 type capillary chromatographic column, improves precision so further and reduces cost.
Operation principle of the present utility model is as follows: accurately weighed about 5g sample by sampler, it is placed in polypropylene centrifuge tube, add 2mol/L ammonium acetate buffer solution 20 milliliters, ultrasonic 20min in the middle of ice-water bath, then the centrifugal 10min of 5000r/min, supernatant pours new polypropylene centrifuge tube into, is simultaneously introduced 20ml normal hexane, vibration mixing.Centrifugal by centrifugal agitator makes normal hexane and aqueous phase layering, draws lower floor's aqueous phase with syringe, does process to be clean.The aqueous phase of absorption is put in the extraction column of the mixed type cation exchange solid phase activated, make its naturally lower in atmospheric conditions, then respectively with the 0.1mol/L hydrochloric acid of 3ml, 3ml water, 50% methanol of 3ml, normal hexane drip washing extraction column, finally carry out eluting with eluent and collect, at 50 DEG C, nitrogen dries up, 1ml mobile phase constant volume, after finally crossing organic filter membrane of 0.22 μm, for liquid chromatograph-mass spectrometer.The good liquid of 10ml pre-treatment drawn by injector, enters in quantitative loop, mobile phase ratio respectively 80% trifluoroacetic acid aqueous solution and 20% 0.2% formic acid water, through C18 type capillary chromatographic column, column temperature 35 DEG C, be easily separated when flow velocity 0.2ml/min.Material after chromatographic column separates enters into mass detector, Mass Spectrometry Conditions: ionization mode sprays ionization for cation, and multiple-reaction monitoring, collision gas is argon, and atomization gas and dry gas are nitrogen.Extract container is used for storing extract.Eluate container is used for storing eluent.Liquid chromatograph-mass spectrometer is for Determination of Clenbuterol Residues in analyzing animal derived food, and mass detector is used for detecting Determination of Clenbuterol Residues in animal-derived food.First centrifuge tube and the second centrifuge tube make the residual of kelengtelu in animal-derived food be completely separated out.
This utility model is suitable to substantial amounts of analysis sample, simple to operate, convenience of calculation, does not need to measure correction factor, is suitable to automatically analyze, and is easily achieved, cheap.
Particular embodiments described above; to of the present utility model solving the technical problem that, technical scheme and beneficial effect further described; it is it should be understood that; the foregoing is only specific embodiment of the utility model; it is not limited to this utility model; all within spirit of the present utility model and principle, any amendment of making, equivalent replacement, improvement etc., should be included within protection domain of the present utility model.
Claims (3)
- null1. one kind to the mensuration system of Determination of Clenbuterol Residues in animal-derived food，It is characterized in that，It includes sampler、First centrifuge tube、Low temperature temperature control instrument、Ultrasonic generator、Second centrifuge tube、Centrifugal agitator、Extraction column、Extract container、Eluate container、Organic filter membrane、Injector、Quantitative loop、Chromatographic column、Liquid chromatograph-mass spectrometer、Mass detector，Sampler and the first centrifuge tube are connected，The upper end of the first centrifuge tube is connected with the upper end of the second centrifuge tube，Second centrifuge tube is positioned at low temperature temperature control instrument，Low temperature temperature control instrument is positioned at ultrasonic generator，Second centrifuge tube is fixed in centrifugal agitator，The lower end of the second centrifuge tube is connected with the upper end of extraction column，Extract container is all connected with extraction column upper end with eluate container，Extraction column lower end is connected with on the upside of organic filter membrane，Injector is positioned on the downside of organic filter membrane，The injector other end is connected with one end of quantitative loop，One end of quantitative loop is connected with one end of chromatographic column，The other end of chromatographic column is connected with liquid chromatograph-mass spectrometer，Chromatographic column、Liquid chromatograph-mass spectrometer is all connected with mass detector.
- 2. as claimed in claim 1 to the mensuration system of Determination of Clenbuterol Residues in animal-derived food, it is characterised in that described chromatographic column is C18 type capillary chromatographic column.
- 3. as claimed in claim 1 to the mensuration system of Determination of Clenbuterol Residues in animal-derived food, it is characterised in that described first centrifuge tube and the second centrifuge tube are all polypropylene centrifuge tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620083391.2U CN205374398U (en) | 2016-01-28 | 2016-01-28 | Remain measurement of quantities system to clenbuterol among animal -derived food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620083391.2U CN205374398U (en) | 2016-01-28 | 2016-01-28 | Remain measurement of quantities system to clenbuterol among animal -derived food |
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| Publication Number | Publication Date |
|---|---|
| CN205374398U true CN205374398U (en) | 2016-07-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620083391.2U Expired - Fee Related CN205374398U (en) | 2016-01-28 | 2016-01-28 | Remain measurement of quantities system to clenbuterol among animal -derived food |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114487221A (en) * | 2020-12-22 | 2022-05-13 | 苏州申测检验检测中心有限公司 | Detection apparatus for clenbuterol residual quantity in survey animal food |
-
2016
- 2016-01-28 CN CN201620083391.2U patent/CN205374398U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114487221A (en) * | 2020-12-22 | 2022-05-13 | 苏州申测检验检测中心有限公司 | Detection apparatus for clenbuterol residual quantity in survey animal food |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160706 Termination date: 20170128 |
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| CF01 | Termination of patent right due to non-payment of annual fee |