CN106755068B - Application of the amino acid transport gene OsANT1 in rice breeding - Google Patents
Application of the amino acid transport gene OsANT1 in rice breeding Download PDFInfo
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- CN106755068B CN106755068B CN201611110825.4A CN201611110825A CN106755068B CN 106755068 B CN106755068 B CN 106755068B CN 201611110825 A CN201611110825 A CN 201611110825A CN 106755068 B CN106755068 B CN 106755068B
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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Abstract
The invention discloses amino acid transport genesOsANT1Application in rice breeding, belongs to plant genetic engineering field.OsANT1The amino acid sequence of DNA encoding the protein is as shown in SEQ ID NO.1, and cDNA sequence is as shown in SEQ ID NO.2.The present invention passes through building riceOsANT1The overexpression plant of gene, discovery improveOsANT1The expression of gene can be such that normal rice root long, radical, plant height and fresh weight increases, and biomass improves.Plant is interfered by building, discovery passes through reductionOsANT1Gene expression can be such that normal rice root long, radical and fresh weight reduces.ThereforeOsANT1Gene can be used in rice breeding promoting paddy growth, improve rice biological amount.OsANT1Gene has important application value in terms of illustrating that amino acid transport influences plant growth and growth course.
Description
Technical field
The invention belongs to plant genetic engineering fields, and in particular to amino acid transport geneOsANT1In rice breeding
Using.
Background technique
Plant obtains nitrogen by absorbing ammonia, nitrate anion, amino acid, soluble peptide in soil etc.；The absorption of nitrogen and
Transhipment relies primarily on ammonium root transport protein (AMT), nitrate anion transport protein (NRT), amino acid transport proteins (AAT), peptide transport
The transport proteins such as albumen (PTR) complete (Williams L, Miller A. Transporters responsible for
the uptake and partitioning of nitrogenous solutes. Annu Rev Plant Biol and
Plant Mol Biol, 2001,52:659-688.).Ammonium passes through glutamine synthelase after absorbing by plant AMT again
(GS) and glutamate synthase (GOGAT) synthesizes glutamine and glutamic acid, the latter further form other amino acid (Sonoda
Y, Ikeda A, Saiki S, et al. Feedback regulation of the ammonium transporter
gene family AMT1 by glutamine in rice. Plant Cell Physiol, 2003, 44: 1396-
1402.).Plant can absorb ring by the NRT2 of high affine movement system (HATS) and the NRT1 of low affine movement system (LATS)
Nitrate in border forms ammonium by nitrate reductase (NR) and nitrite reductase (NiR) reduction, further forms amino acid
(Paungfoo-Lonhienne C, Lonhienne T G, Rentsch D, et al. Plants can use
protein as a nitrogen source without assistance from other organisms. PNAS,
2008,105:4524-4529.).
In higher plant, AAT is a kind of transmembrane protein, by amino acid from it is extracellular be transported to it is intracellular, while also in amino acid
Long distance transportation, cause of disease reaction and abiotic stress etc. play an important role (Tegeder M. Transporters
for amino acids in plant cells: some functions and many unknowns. Curr Opin
Plant Biol, 2012,15:1-7.).AAT gene is divided into two superfamilies: APC(amino acid, polyamines and choline turn
Fortune) superfamily and AAAP(amino acid/auxin permease) superfamily.APC superfamily is divided into three subfamilies: CATs(sun from
Sub- amino acid transporter) family, ACTs(amino acid/choline transport albumen) family and PHSs(polyamines, H+ cotransport egg
It is white) family.AAAP superfamily is divided into six subfamilies: AAPs(amino acid permease) family, LHTs(lysine and histidine turn
Transport albumen) family, ProTs(proline transport protein) family, GATs(gamma-amino acid butyric acid, GABA) family, AUXs(growth
Plain transport protein) family and ANTs(aromatic series and neutral amino acid transporter) family (Fischer WN, Andre B,
Rentsch D, et al. Amino acid transport in plants. Trends Plant Sci, 1998, 3:
188-195.).
85 AAT family members (Zhao H, Ma H, Yu L, et al. Genome- is found in rice genome altogether
wide survey and expression analysis of amino acid transporter gene family in
Rice (Oryza sativa L.) PLoS ONE, 2012,7:e49210.).Research is foundOsAAT5、OsAAT7、OsAAT24、OsAAT49WithOsAAT60T-DNA insertion mutation body yield of brown rice and plant dry weight decline, it was demonstrated that AAT
Nitrogen accumulation and Carbon and nitrogen allocation important role (Lu Y, Song Z, Lu K, et al. Molecular to rice
characterization, expression and functional analysis of the amino acid
transporter gene family (OsAATs) in rice. Acta physiol Plant, 2012, 34: 1943-
1962.).Research finds overexpressionOsAAP6It will increase shelf stability albumen and amino acid content in rice grain, so as to improve
Rice nutrition and flavor (Peng B, Kong H, Li Y, et al. OsAAP6 functions as an important
regulator of grain protein content and nutritional quality in rice. Nat
Commun, 2014,5:doi:10.1038.).Amino acid transporter to the Amino Acid Absorptions of the various plants such as rice,
Transhipment and storage play an important role.Report at present about rice AAT family member research is seldom,ANTGene expression
Albumen prevailing traffic neutral amino acid, at present rice amino acid transport familyOsANT1Growth and development of the albumen of gene to rice
There is not any research at present.Present invention discover thatOsANT1The influence of gene pairs rice biological amount has more important role, can apply
It is improved in plant plant type.
Summary of the invention
It is an object of the invention to solve problems of the prior art, amino acid transport gene is providedOsANT1In water
Application in rice breeding.
The purpose of the invention is achieved by the following technical solution:
The present invention is with the amino acid transport gene of riceOsANT1For object, cloned from being spent in rice in 11OsANT1's
CDNA sequence.Pass through buildingOsANT1The overexpression vector of gene, the genetic transforming method mediated using Agrobacterium EHA105 will
Overexpression vector is imported in normal japonica rice variety and is spent in 11, is obtainedOsANT1The overexpression plant that gene expression amount rises, super table
It is significantly improved compared with spending 11 in control wild type up to the root long of plant, radical, plant height and fresh weight.It constructs simultaneouslyOsANT1Base
The interference carrier of cause, by interference carrier import in spend in 11, obtainOsANT1The interference plant of gene, root long, radical and fresh
Weight significantly reduces compared with spending 11 in.These results indicate that passing through raisingOsANT1Gene expression can promote paddy growth,
Increase biomass；OsANT1Gene has important application in terms of illustrating that amino acid transport influences plant growth and growth course
Value.
Based on present invention discover thatOsANT1The function of gene can be used in rice breeding.The rice breeding is
Rice root long, radical, plant height and fresh weight are improved, to promote paddy growth, improves rice object amount.It can specifically be mentioned by overexpression
It is highOsANT1The expression of gene increases rice root long, radical, plant height and fresh weight, achievees the purpose that improve rice biological amount.Institute
It statesOsANT1The amino acid sequence of the OsANT1 albumen of gene coding is as shown in SEQ ID NO.1；DescribedOsANT1Gene
CDNA sequence preferably as shown in SEQ ID NO.2.
It is construed as, (i.e. not in the activated centre of albumen) under the premise of not influencing OsANT1 protein active, ability
It is one or several that field technique personnel can carry out various substitutions, additions and/or deletions to amino acid sequence shown in SEQ ID NO.1
Amino acid obtains the amino acid sequence with same function.Therefore, OsANT1 albumen further includes amino acid shown in SEQ ID NO.1
Sequence is substituted, replaces and/or increases that one or several amino acid obtain has same active protein.In addition, Ying Li
Solution, it is contemplated that the degeneracy of codon and the preferences of different plant species codon, those skilled in the art can according to need
The codon expressed using suitable particular species.
Advantages of the present invention and effect:
(1) present invention clonesOsANT1Gene makes rice root long, radical, plant height and fresh weight enhancing after improving expression, says
It is brightOsANT1Gene pairs raising rice biological amount is more apparent, therefore, is improved by technique for gene engineeringOsANT1The expression of gene
It can be improved phytomass.It is not only does this facilitate and cultivates high-yield rice under the conditions of nitrogen by normally applying, combination can also be passed through
The breed improvement of molecular breeding progress plant.
(2)OsANT1The successful clone of gene further demonstrates weight of the amino acid transport family in nitrogen absorption process
It acts on, there is important meaning to the biological function for illustrating amino acid transport family, in addition to further appreciating that plant nitrogen generation
Thank to approach, improving nitrogen absorption efficiency has great impetus.
(3) although being cloned into some nitrogen nutrition approach at present influences the gene of plant growth, to plant growth
The molecular mechanism of development is still unclear.And what the present invention clonedOsANT1Gene can be improved the biomass of rice, plant to determining
The key factor of object volume increase has great impetus.
Detailed description of the invention
Fig. 1 be spend 11 in control,OsANT1Gene overexpress 2 strains of plant andOsANT1Gene interferes 2 strains of plant
The whole strain phenotypic map of system.
Fig. 2 be spend 11 in control,OsANT1Gene overexpress 2 strains of plant andOsANT1Gene interferes 2 strains of plant
SystemOsANT1The statistics histogram of gene expression amount, data carry out variable analysis (ANOVA) using SPSS software, use
Duncan ' s carries out significance difference analysis in 0.05 level, and it is poor to have between different group lowercase (a, b, c) expressions
It is different significant.
Fig. 3 is to spend 11 Hes in controlOsANT1Gene overexpress 2 strains of plant andOsANT1Gene interferes 2 strains of plant
It is the statistics histogram of root long, data carry out variable analysis (ANOVA) using SPSS software, use Duncan ' s in 0.05 level
Upper carry out significance difference analysis has significant difference between the expression of different group lowercases (a, b, c).
Fig. 4 be spend 11 in control,OsANT1Gene overexpress 2 strains of plant andOsANT1Gene interferes 2 strains of plant
It is the statistics histogram of radical, data carry out variable analysis (ANOVA) using SPSS software, use Duncan ' s in 0.05 level
Upper carry out significance difference analysis has significant difference between the expression of different group lowercases (a, b, c).
Fig. 5 be spend 11 in control,OsANT1Gene overexpress 2 strains of plant andOsANT1Gene interferes 2 strains of plant
It is the statistics histogram of plant height, data carry out variable analysis (ANOVA) using SPSS software, use Duncan ' s in 0.05 level
Upper carry out significance difference analysis has significant difference between the expression of different group lowercases (a, b).
Fig. 6 be spend 11 in control,OsANT1Gene overexpress 2 strains of plant andOsANT1Gene interferes 2 strains of plant
It is the statistics histogram of fresh weight, data carry out variable analysis (ANOVA) using SPSS software, use Duncan ' s in 0.05 level
Upper carry out significance difference analysis has significant difference between the expression of different group lowercases (a, b, c).
Specific embodiment
Below with reference to embodiment, the present invention will be further described in detail, and embodiments of the present invention are not limited thereto.
Unless otherwise specified, the conventional means that technological means used in following embodiments is well known to those skilled in the art；Used
Experimental method is conventional method, and can according to described recombinant technique (referring to molecular cloning, laboratory manual, second edition,
CSH Press, Cold SpringHarbor, New York) it completes；Material, reagent used etc., are commercially available.
Embodiment 1OsANT1The building of gene overexpression plant
It extracts in rice and spends 11 RNA, and its reverse transcription is utilized into primer pair at cDNA:
F1:5'-AGATCTATGGAGGGGGCGGCGCCGCTTCT-3'(BglII),
R1:5'-CTTAAGCTGCAGTTGAATCCTGCATTGTGG-3'(AflII)；
Pass through PCR amplificationOsANT1After the cDNA of gene, pass throughBglII andAflPCAMBIA-1301 is connected into after II digestion
Carrier (pCAMBIA-1301 carrier is purchased from Cambia company), is constructedOsANT1The overexpression vector of geneOsANT1-
p1301.The genetic transforming method mediated using Agrobacterium EHA105, overexpression vector is imported in normal rice varieties and spends 11
In.
It by the transplanting of obtained all transgenic plants in the basket with soil, periodically waters, fertilising is grown tall about to seedling
When 10cm, 50 plants of Transgenic Rice seedlings are soaked in the 500mL hygromycin solution for being 50mg/L containing concentration of distilled water preparation
In 48 hours, later leaf be green and diastole, growth conditions good stand be positive transgenic plant；And leaf is withered and yellow simultaneously
The plant of curling is negative plant, dead immediately.Positive plant single-strain planting and sowing, until T2 is mould in above-mentioned tide for identifying
It is withered and yellow without any leaf and to crimp be homozygous transgenic plant to get arriving in plain solutionOsANT1Gene overexpresses plant.OsANT1Gene overexpression plant reaches significant difference than spending 11 plant root longs, radical, plant height and fresh weight to increase in control,
As shown in Fig. 1, Fig. 3-6.
It takesOsANT1Gene overexpresses plant leaf, extracts RNA and by its reverse transcription at cDNA, passes through real time fluorescent quantitative
PCR detectionOsANT1Gene is in the expression quantity of overexpression plant, and (Fig. 2) is overexpressed in plant as the result is shownOsANT1The table of gene
It is significantly higher than in control up to amount and spends 11.Real-time fluorescence quantitative PCR the primer pair are as follows:
F2:5'-GGATGCGCCACGCTCTACTG-3',
R2:5'-GCTACAGATCCACCTGCTTGGGAA-3'.
The above results showOsANT1Gene can increase root long, radical, the plant height of rice by the raising of expression quantity
And fresh weight, it is final to improve rice biological amount.
Embodiment 2OsANT1The building of gene interference plant
It extracts in rice and spends 11 RNA, and its reverse transcription is utilized into primer pair at cDNA:
F3:5'-GGTACCGAGTGACTCCGGAAGCCAAGC-3'(KpnI),
R3:5'-GGATCCCAGTAGAGCGTGGCGCATCC-3'(BamH I)；
F4:5'-ACTAGTGAGTGACTCCGGAAGCCAAGC-3'(SpeI),
R4:5'-GAGCTCCAGTAGAGCGTGGCGCATCC-3'(Sac I)；
Respective PCR amplification goes outOsANT1The cDNA segment of gene, by connecting after above-mentioned corresponding digestion with restriction enzyme
Enter pTCK303 carrier, constructsOsANT1The interference expression vector of geneOsANT1-pTCK303.It is situated between using Agrobacterium EHA105
Interference expression vector is imported in normal japonica rice variety and is spent in 11 by the genetic transforming method led.
It by the transplanting of obtained all transgenic plants in the basket with soil, periodically waters, fertilising is grown tall about to seedling
When 10cm, 50 plants of Transgenic Rice seedlings are soaked in the 500mL hygromycin solution for being 50mg/L containing concentration of distilled water preparation
In 48 hours, later leaf be green and diastole, growth conditions good stand be positive transgenic plant；And leaf is withered and yellow simultaneously
The plant of curling is negative plant, dead immediately.Positive plant single-strain planting and sowing, until T2 is mould in above-mentioned tide for identifying
It is withered and yellow without any leaf and to crimp be homozygous transgenic plant to get arriving in plain solutionOsANT1Gene interferes plant.OsANT1Gene interference plant in control than spending 11 plant root longs, radical and fresh weight to substantially reduce, as shown in Fig. 1, Fig. 3-6.
It takesOsANT1Gene interferes plant leaf, extracts RNA and by its reverse transcription at cDNA, passes through real time fluorescent quantitative
PCR detectionOsANT1Gene is in the expression quantity of interference plant, and (Fig. 2) is interfered in plant as the result is shownOsANT1The expression quantity of gene
Than spending 11 reductions in control.Detection of the real-time fluorescence quantitative PCR the primer to same overexpression plant.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
SEQUENCE LISTING
<110>Wuhan Bioengineering Institute
<120>application of the amino acid transport gene OsANT1 in rice breeding
<130> 1
<160> 10
<170> PatentIn version 3.3
<210> 1
<211> 424
<212> PRT
<213> Oryza sativa
<400> 1
Met Glu Gly Ala Ala Pro Leu Leu Ala Ala Arg Gly Glu Glu Glu Val
1 5 10 15
Val Glu Gly Arg Arg Arg Gly Gly Gly Gly Gly Ala Thr Ser Ala Gln
20 25 30
Thr Leu Gly Asn Val Val Val Ser Ile Val Gly Thr Gly Val Leu Gly
35 40 45
Leu Pro Tyr Ala Phe Arg Thr Ala Gly Trp Val Ala Gly Ser Leu Gly
50 55 60
Val Ala Ala Ala Gly Cys Ala Thr Leu Tyr Cys Met Leu Leu Leu Val
65 70 75 80
Asp Cys Arg Asp Lys Leu Glu Glu Lys Glu Ser Glu Glu Thr Tyr His
85 90 95
Gly His Tyr Thr Tyr Gly Asp Leu Gly Glu Lys Cys Phe Gly Thr Ile
100 105 110
Gly Arg Cys Leu Thr Glu Ile Leu Ile Leu Val Ser Gln Ala Gly Gly
115 120 125
Ser Val Ala Tyr Leu Ile Phe Ile Gly Gln Asn Leu His Ser Val Phe
130 135 140
Ser Gln Leu Met Ser Pro Ala Ala Phe Ile Phe Ala Ile Leu Leu Pro
145 150 155 160
Met Gln Ile Ala Leu Ser Phe Ile Arg Ser Leu Ser Ser Leu Ser Pro
165 170 175
Phe Ser Ile Phe Ala Asp Val Cys Asn Val Leu Ala Met Ala Ile Val
180 185 190
Ile Lys Glu Asp Leu Gln Leu Phe Asp His Pro Phe Ala Asn Arg Ser
195 200 205
Ala Phe Asn Gly Leu Trp Ala Ile Pro Phe Thr Phe Gly Val Ala Val
210 215 220
Phe Cys Phe Glu Gly Phe Ser Met Thr Leu Ala Leu Glu Ser Ser Met
225 230 235 240
Ala Glu Arg Arg Lys Phe Arg Trp Val Leu Ser Gln Ala Val Val Gly
245 250 255
Ile Ile Ile Val Tyr Ala Cys Phe Gly Val Cys Gly Tyr Leu Ala Tyr
260 265 270
Gly Glu Ala Thr Lys Asp Ile Ile Thr Leu Asn Leu Pro Asn Ser Trp
275 280 285
Ser Ser Ala Ala Val Lys Val Gly Leu Cys Ile Ala Leu Val Phe Thr
290 295 300
Phe Pro Val Met Met His Pro Ile His Glu Ile Val Glu Glu Arg Phe
305 310 315 320
Gln Ser Ser Gly Cys Phe Pro Lys Leu Ser His Lys Val Arg Gly Ala
325 330 335
Glu Trp Val Gly Leu His Ser Ser Arg Ile Val Met Val Thr Ile Leu
340 345 350
Ser Val Val Ala Ser Phe Ile Pro Ala Phe Gly Ser Phe Ile Ser Phe
355 360 365
Val Gly Ser Thr Val Cys Ala Leu Leu Ser Phe Val Leu Pro Thr Ile
370 375 380
Phe His Leu Ser Ile Val Gly Ser Ser Met Ser Pro Trp Arg Arg Trp
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Gly Asp Tyr Gly Phe Leu Leu Phe Gly Leu Gly Phe Ala Gly Tyr Gly
405 410 415
Leu Ile Arg Ala Leu Phe Ser His
420
<210> 2
<211> 1275
<212> DNA
<213> Oryza sativa
<400> 2
atggaggggg cggcgccgct tctggcggcg cgtggggagg aggaggtggt ggaggggagg 60
cggcgcgggg gagggggcgg ggcgacgtcg gcgcagacgc tggggaacgt ggtggtgtcc 120
atcgtgggga ccggggtgct cggcctcccc tacgcgttcc gcaccgccgg ctgggtcgcc 180
ggctcgctcg gcgtcgccgc cgccggatgc gccacgctct actgcatgct cctcctcgtt 240
gactgcagag ataaattgga agagaaagaa tctgaagaaa cctaccatgg tcactataca 300
tatggtgatt tgggtgaaaa gtgttttggc actataggtc gatgcttgac agaaatcctc 360
atccttgttt cccaagcagg tggatctgta gcttatctaa tattcattgg ccaaaatctt 420
cattctgtct tcagccagtt gatgtcacca gctgccttca tctttgccat tctcctgcct 480
atgcaaatcg ctctatcatt catccgttca ctatcttctc tctccccatt cagcatattt 540
gcagacgtct gcaatgttct tgctatggca attgttatca aagaggatct tcagcttttt 600
gatcacccat ttgcgaatag aagtgctttc aatggacttt gggcgatacc attcactttt 660
ggagttgcgg tcttctgctt cgaaggattc agtatgactt tggcactaga atcatcaatg 720
gcagaacgga gaaaattccg ctgggtactt tctcaagcag ttgtcggtat cataattgtc 780
tatgcatgtt ttggagtttg tggttactta gcctatgggg aggccactaa agacatcata 840
acacttaatc ttcccaatag ttggtcttct gctgccgtta aggttggtct ttgcatagca 900
ctggtattca catttccagt catgatgcac ccaatccacg agattgtcga ggaaagattc 960
caatcaagtg gatgtttccc gaagctctcg cacaaggttc gcggggctga atgggtgggc 1020
ctgcactcaa gccgcatcgt catggtaact atcctgtctg tggtggcatc cttcatacct 1080
gcgttcgggt ccttcatctc ctttgtcggg agcacggttt gcgcgctgct ctccttcgtg 1140
ctgcctacga tcttccatct gagcattgtg ggttcctcga tgagtccgtg gcgacgctgg 1200
ggggactacg gtttccttct cttcggcctt ggttttgcgg gctacgggct cattagggct 1260
ctcttctcac attga 1275
<210> 3
<211> 29
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<213> Artificial
<220>
<223>primers F 1
<400> 3
agatctatgg agggggcggc gccgcttct 29
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<213> Artificial
<220>
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cttaagctgc agttgaatcc tgcattgtgg 30
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<212> DNA
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<220>
<223>primers F 2
<400> 5
ggatgcgcca cgctctactg 20
<210> 6
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<212> DNA
<213> Artificial
<220>
<223>primer R2
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gctacagatc cacctgcttg ggaa 24
<210> 7
<211> 27
<212> DNA
<213> Artificial
<220>
<223>primers F 3
<400> 7
ggtaccgagt gactccggaa gccaagc 27
<210> 8
<211> 26
<212> DNA
<213> Artificial
<220>
<223>primer R3
<400> 8
ggatcccagt agagcgtggc gcatcc 26
<210> 9
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<213> Artificial
<220>
<223>primers F 4
<400> 9
actagtgagt gactccggaa gccaagc 27
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<212> DNA
<213> Artificial
<220>
<223>primer R4
<400> 10
gagctccagt agagcgtggc gcatcc 26
Claims (4)
- Application of the 1.OsANT1 gene in rice breeding, it is characterised in that: the rice breeding is to improve rice biological amount； The rice biological amount includes rice root long, radical, plant height, fresh weight；The OsANT1 albumen of the OsANT1 gene coding Amino acid sequence as shown in SEQ ID NO.1.
- 2. application according to claim 1, it is characterised in that: the expression by improving OsANT1 gene increases rice root Length, radical, plant height and fresh weight, to improve rice biological amount.
- 3. application according to claim 1, it is characterised in that: the expression by reducing OsANT1 gene reduces rice root Long, radical and fresh weight, to reduce rice biological amount.
- 4. application according to claim 1, it is characterised in that: the cDNA sequence of the OsANT1 gene such as SEQ ID Shown in NO.2.
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| US6165792A (en) * | 1998-08-20 | 2000-12-26 | E. I. Du Pont De Nemours And Company | Amino acid transporters |
| WO2003066879A2 (en) * | 2002-02-01 | 2003-08-14 | Monsanto Technology Llc | Amino acid transporters |
| ATE544862T1 (en) * | 2005-12-23 | 2012-02-15 | Arcadia Biosciences Inc | RICE PROMOTER SEQUENCE AND METHOD FOR USE THEREOF |
| CN101386867A (en) * | 2008-09-19 | 2009-03-18 | 华中农业大学 | Use of gene OsAAT1 in controlling rice grain quality |
| CN101386865A (en) * | 2008-09-19 | 2009-03-18 | 华中农业大学 | Use of gene OsAAT2 in controlling rice grain quality |
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