CN108034661B - Application of OsNPF8.8b gene in improving rice yield and nutrition quality - Google Patents
Application of OsNPF8.8b gene in improving rice yield and nutrition quality Download PDFInfo
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
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Abstract
The invention discloses application of an OsNPF8.8b gene in improving rice yield and nutrition quality, and belongs to the field of plant genetic engineering. The amino acid and cDNA sequences of the OsNPF8.8b gene coding protein are shown in SEQ ID NO.1 and 2. According to the invention, by constructing rice OsNPF8.8b gene overexpression plants, the fact that the improvement of the expression of the OsNPF8.8b gene can increase the tillering number and effective spike of rice, increase the grain filling number of single plants and the dry weight of single grain filling, and increase the globulin content in rice is found. Through constructing mutant plants, the fact that OsNPF8.8b gene expression is knocked out is found to reduce the tillering number of rice, reduce the grain filling number of single plants and the dry weight of single grain filling, and reduce the globulin content in rice. Therefore, the OsNPF8.8b gene can be used for promoting the improvement of the yield and the quality of rice, and has important application in the aspects of improving the nitrogen utilization efficiency of the rice and improving the quality of the rice.
Description
Technical Field
The invention belongs to the field of plant genetic engineering, and particularly relates to application of an OsNPF8.8b gene in improving rice yield and nutritional quality.
Background
Plants obtain nitrogen by absorbing ammonium, nitrate, amino acids, soluble peptides, etc. in soil; nitrogen uptake and transport is mainly achieved by transporters such as ammonium transport protein (AMT), nitrate transport protein (NRT), amino acid transport protein (AAT), peptide transport Protein (PTR) (Williams L, Miller A. transporters response for the uptake and purification of nitrogenes solutions. annular Review of Plant physiology and Plant Molecular Biology,2001,52: 659. sup. 688.). Ammonium is taken up by the plant AMT and then glutamine and glutamate are synthesized by Glutamine Synthetase (GS) and glutamate synthase (GOGAT), which in turn further form other amino acids (Sonoda Y, Ikeda A, Saiki S, et al. feedback regulation of the ammonium transporter gene family AMT1by glutamine in rice. plant cell physiology,2003,44: 1396-. Plants can absorb environmental nitrates via NRT2 from the High Affinity Transport System (HATS) and NRT1 from the Low Affinity Transport System (LATS), form ammonium upon reduction by Nitrate Reductase (NR) and nitrite reductase (NiR), and further form amino acids (Paungfoo-Lonhienne C, Lonhienne T G, Rentsch D, et.
The NPF family of nitrogen transporters includes the NRT1 and PTR subfamilies, where different members transport nitrate, oligopeptides, amino acids, etc. at different sites in the plant and play different roles in plant growth and development (Rentsch D, Schmidt S, TegederM. transporters for uptake and allocation of organic nitrogen compounds in plants Febs Letters,2007,581: 2281. sup. 2289.). OsNPF2.2 mediates the unloading of xylem nitrate and affects rice plant growth (Li Y, Ouyang J, Wang Y, et al, precipitation of the rice nitrate OsNPF2.2 promoters root-to-shoot nitrate and vascular assessment scientific reports 2015,5: 9635.). OsNPF7.2 has a low affinity for nitrate transport and can influence plant growth (Hu R, Qiu D, Chen Y, et al. knock-down of a toplast localization-after nitrate transport OsNPF7.2 artifacts with growth under high sensitivity in plant science 2016, 7.).
While nitrogen nutrition is known to promote plant growth and development, there is currently no systematic understanding of what types of plants nitrogen nutrition affects growth and development. In addition, more than 80 members of the rice NPF family exist, nitrogen nutrition responds through which member of the NPF gene family, nitrogen nutrition transportation is mediated at what part, and therefore, what type of plant growth and development are influenced, whether the rice nutrition quality is influenced, and what quality is influenced are almost unknown at present. Therefore, the excavated NPF family may have nitrogen efficient transport genes, especially nitrogen transport key genes capable of controlling the agronomic characters of rice, and is beneficial to the cultivation of high-yield rice varieties. After long-term research, the invention discovers that two splicing types, namely OsNPF8.8a and OsNPF8.8b, exist after the transcription of the OsNPF8.8b gene of the NPF family, wherein the OsNPF8.8b plays an important role in improving the yield and the nutritional quality of rice, can be applied to the improvement of the nitrogen utilization efficiency of plants and the improvement of the quality of rice, and is used for cultivating high-yield and high-quality rice varieties.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides application of an OsNPF8.8b gene in improving rice yield and nutrition quality.
The purpose of the invention is realized by the following technical scheme:
the invention takes the rice OsNPF8.8b gene as an object, and clones the cDNA sequence of the OsNPF8.8b from rice middle flower 11. The overexpression vector is introduced into the normal japonica rice variety middle flower 11 by constructing the overexpression vector of the OsNPF8.8b gene and adopting an agrobacterium EHA105 mediated genetic transformation method, so that an overexpression plant of the OsNPF8.8b gene is obtained, the tillering number, the effective spike number, the grain filling dry weight and the like of the overexpression plant are remarkably improved compared with those of a control wild type middle flower 11, and the globulin content in rice is also improved compared with that of the control. And meanwhile, a CRISPR gene knockout vector of the OsNPF8.8b gene is constructed, and the CRISPR gene knockout vector is introduced into the middle flower 11 to obtain a mutant plant of the OsNPF8.8b gene, wherein the tillering number, the effective spike number, the grain filling dry weight and the like of the mutant plant are obviously reduced compared with those of the middle flower 11, and the globulin content in rice is also reduced compared with that in a control. These results indicate that by increasing the expression of the OsNPF8.8b gene, the increase of the rice yield and the nutritional quality can be promoted; the OsNPF8.8b gene has application value in improving the nitrogen utilization efficiency and the nutritional quality of rice, and can be applied to rice variety improvement through molecular breeding.
Based on the functions of the OsNPF8.8b gene discovered by the invention, the OsNPF8.8b gene can be used for breeding rice so as to promote the improvement of rice yield and nutrition quality. The method can be realized by genetic engineering, namely the expression of the OsNPF8.8b gene is improved, so that the tillering number, the effective spike number, the single-plant grouted seed dry weight, the globulin content in rice and the like of the rice are increased, and the purposes of improving the nitrogen utilization efficiency of the rice, improving the rice yield and improving the rice nutritional quality are achieved.
The amino acid sequence of the OsNPF8.8b protein coded by the OsNPF8.8b gene is shown as SEQ ID NO. 1; the cDNA sequence of the OsNPF8.8b gene is preferably shown as SEQ ID NO. 2.
It is understood that the amino acid sequence shown in SEQ ID NO.1 can be variously substituted, added and/or deleted by one or more amino acids by those skilled in the art to obtain an amino acid sequence having equivalent functions without affecting the activity of the OsNPF8.8b protein (i.e., without being at the active center of the protein). Therefore, the OsNPF8.8b protein also comprises a protein with equivalent activity, which is obtained by substituting, replacing and/or adding one or more amino acids in the amino acid sequence shown in SEQ ID NO. 1. Furthermore, it will be appreciated that, given the degeneracy of codons and the preference of codons for different species, one skilled in the art can use codons suitable for expression in a particular species as desired.
The invention has the advantages and effects that:
(1) after the expression of the OsNPF8.8b gene cloned by the invention is improved, tillering of rice, effective spike, single-plant grain filling number and single-plant grain filling dry weight can be increased, which shows that the OsNPF8.8b gene has obvious effect on improving the yield of the rice, so that the plant yield can be improved by improving the expression of the OsNPF8.8b gene through a genetic engineering technology. This not only contributes to the cultivation of high-yielding rice by reducing the use of nitrogen fertilizers, but also contributes to the variety improvement of plants by molecular breeding.
(2) The cloned OsNPF8.8b gene can increase globulin in rice after being improved and expressed, which shows that the OsNPF8.8b gene has obvious effect on improving the nutritional quality of rice, the successful cloning of the gene further proves the important effect of the inherent gene of plants on the quality, and has important significance on the elucidation of the inherent gene of rice on the improvement of the rice quality.
(3) Although some genes in the nitrogen nutrition pathway have been cloned so far, it is still unclear how the nitrogen nutrition pathway exerts an effect on plants. The OsNPF8.8b gene cloned by the invention can improve the yield and the quality of rice and has great promotion effect on determining key factors of high yield and high quality of plants.
Drawings
FIG. 1 is a table diagram of the entire plants of 3 lines (OsNPF8.8b-OE) of a control flower 11(WT) and an OsNPF8.8b gene overexpression plant T2 generation and an OsNPF8.8b gene mutant plant T2 generation (OsNPF8.8b-C) under field planting.
FIG. 2 is a statistical chart of tillering numbers of 3 lines (OsNPF8.8b-OE) of a control flower 11(WT) and an OsNPF8.8b gene overexpression plant T2 generation and an OsNPF8.8b gene mutant plant T2 generation (OsNPF8.8b-C) under field planting. Data were analyzed for variation using SPSS software (ANOVA) and for significance of differences using Duncan's at three levels, 0.05, 0.01, and 0.001, with the three levels being marked as a, and a.
FIG. 3 is the effective ear statistical chart of control flower 11(WT), 3 lines of OsNPF8.8b gene overexpression plant T2 generation (OsNPF8.8b-OE) and OsNPF8.8b gene mutant plant T2 generation (OsNPF8.8b-C) under field planting. Data were analyzed for variation using SPSS software (ANOVA) and for significance of differences using Duncan's at three levels, 0.05, 0.01, and 0.001, with the three levels being marked as a, and a.
FIG. 4 is a table graph of the number of grouted seeds of individual plants of flower 11(WT), OsNPF8.8b gene overexpression plant T2 generation 3 lines (OsNPF8.8b-OE) and OsNPF8.8b gene mutant plant T2 generation (OsNPF8.8b-C) under field planting.
FIG. 5 is a statistical chart of the number of grouted seeds of individual plants of flower 11(WT), 3 lines of OsNPF8.8b gene overexpression plant T2 generation (OsNPF8.8b-OE) and OsNPF8.8b gene mutant plant T2 generation (OsNPF8.8b-C) under field planting. Data were analyzed for variation using SPSS software (ANOVA) and for significance of differences using Duncan's at three levels, 0.05, 0.01, and 0.001, with the three levels being marked as a, and a.
FIG. 6 is a statistical chart of dry weights of individual grouted grains of 3 lines (OsNPF8.8b-OE) of T2 generation of control medium flower 11(WT) and OsNPF8.8b gene overexpression plant and T2 generation (OsNPF8.8b-C) of OsNPF8.8b gene mutant plant under field planting. Data were analyzed for variation using SPSS software (ANOVA) and for significance of differences using Duncan's at three levels, 0.05, 0.01, and 0.001, with the three levels being marked as a, and a.
FIG. 7 is a graph showing the results of detecting the OsNPF8.8b gene expression levels of 3 lines (OsNPF8.8b-OE) of a control medium flower 11(WT) and an OsNPF8.8b gene overexpression plant T2 generation under field planting. Data were analyzed for variation using SPSS software (ANOVA) and for significance of differences using Duncan's at three levels, 0.05, 0.01, and 0.001, with the three levels being marked as a, and a.
FIG. 8 is a graph showing the results of the detection of globulin content in rice of 3 lines (OsNPF8.8b-OE) of control middle flower 11(WT) and OsNPF8.8b gene overexpression plant T2 generation and OsNPF8.8b gene mutant plant T2 generation (OsNPF8.8b-C) under field planting. Data were analyzed for variation using SPSS software (ANOVA) and for significance of differences using Duncan's at three levels, 0.05, 0.01, and 0.001, with the three levels being marked as a, and a.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art; the experimental procedures used are conventional and can be carried out according to recombinant techniques already described (see molecular cloning, A laboratory Manual, 2 nd edition, Cold spring harbor laboratory Press, Cold spring harbor, N.Y.; Ma X et al, A robust CRISPR/Cas9system for containment, high-efficiency multiplex genome editing in monocot and dicotplants. mol plant.2015,8(8): 1274-1284.); the materials, reagents and the like used are all commercially available.
Example 1 construction of OsNPF8.8b Gene overexpression plants
Extracting RNA of rice middle flower 11, reversely transcribing the RNA into cDNA, and performing primer pair:
F1：5'-AGATCTATGGCAAATGAGGAGCAGCGGCAG-3'(Bgl II)，
R1：5'-CTTAAGTCAAGTGGTAGGCAGCTTGCGTTTG-3'(Afl II)；
after cDNA of the OsNPF8.8b gene is amplified by PCR (polymerase chain reaction) by using F1 and R1 primers respectively, the cDNA is cut by Bgl II and Afl II and then is connected into a pCAMBIA-1301 vector (the pCAMBIA-1301 vector is purchased from Cambia company), and the overexpression vector OsNPF8.8b-p1301 of the OsNPF8.8b gene is constructed. The overexpression vectors are respectively introduced into the flowers 11 of the normal rice variety by adopting an agrobacterium EHA105 mediated genetic transformation method.
Transplanting all the obtained transgenic seedlings into a basket with soil, watering and fertilizing at regular intervals, soaking 50 rice transgenic seedlings into a hygromycin solution with the concentration of 50mg/L prepared by 500mL of distilled water for 48 hours when the seedlings are about 10cm long, and then taking plants with green leaves and good growth states as positive transgenic plants; while plants with withered and yellow leaves and curly leaves were negative plants and died immediately. And planting and harvesting a single positive plant until a homozygous transgenic plant without withered and yellow leaves and curling in the hygromycin solution is identified in the T2 generation, and obtaining an over-expression plant of the OsNPF8.8b gene. Soaking the over-expression plant and the seeds of the middle flower 11 in distilled water for 3 days on a culture dish, culturing for 7 days, transferring to a rice nutrient solution for culturing for 20 days, planting in a field, counting the tillering number, the effective spike, the single plant grouting seed number and the single plant grouting seed dry weight, and obtaining the results shown in figures 1-6. As can be seen from the graphs in FIGS. 1-6, in the field culture, compared with the control flower 11 plant, the T2 generation of the OsNPF8.8b gene overexpression plant has the advantages that the tillering number, the effective spike, the single plant grouted seed number and the single plant grouted seed dry weight are increased, and the difference of the three lines is obvious. The expression level of the OsNPF8.8b gene of the over-expression plant is detected, and the expression of the OsNPF8.8b gene is improved compared with that of a control, as shown in FIG. 7. The rice nutrition quality measurement shows that the globulin content in the rice of the over-expression plant is improved compared with the control, and the result is shown in figure 8.
Example 2 construction of OsNPF8.8b Gene mutant plants
F2：5'-AGGTAGTTTGGCGTCAGTAATGG-3'，
F3：5'-ACTGATCCATTGCCTGTGTATGG-3'。
Using the above target sequence, a gene knockout vector OsNPF8.8b-C of OsNPF8.8b gene was constructed (see Ma X et al, A robust CRISPR/Cas9system for containment, high-efficiency multiplex gene editing in monocot and dicotplants. mol plant.2015,8(8): 1274. sup. -. The gene knockout expression vector is introduced into the flower 11 of the normal japonica rice variety by adopting an agrobacterium EHA105 mediated genetic transformation method.
Transplanting all the obtained transgenic seedlings into a basket with soil, watering and fertilizing at regular intervals, soaking 50 rice transgenic seedlings into a hygromycin solution with the concentration of 50mg/L prepared by 500mL of distilled water for 48 hours when the seedlings are about 10cm long, then taking plants with green leaves and good diastole and growth states as positive transgenic plants, taking plants with withered and yellow leaves and curling as negative plants, and immediately dying. Sequencing the positive plants at the T1 generation, determining that the genes are knocked out, and harvesting and planting the single plants until the mutant plants are obtained at the T2 generation. Soaking the mutant plant and the seeds of the middle flower 11 in distilled water for 3 days on a culture dish, culturing for 7 days, transferring to a rice nutrient solution for culturing for 20 days, planting in a field, counting the tillering number, the effective spike, the single plant grouting seed number and the single plant grouting seed dry weight, and obtaining the results shown in figures 1-6. As can be seen from the graphs in FIGS. 1-6, in the field culture, compared with the control medium flower 11 plant, the T2 generation OsNPF8.8b gene mutant plant has the advantages that the tillering number, the effective spike, the single plant grouted seed number and the single plant grouted seed dry weight are all reduced compared with the control, and the difference is obvious. The rice nutritional quality measurements found that the globulin content of the rice was reduced in the mutant plants compared to the control, and the results are shown in FIG. 8.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Sequence listing
<110> Wuhan bioengineering college
Application of <120> OsNPF8.8b gene in improving rice yield and nutrition quality
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Gln Pro Glu Glu Gly Pro Tyr Thr Gly Asn Gly Ser Val Asp Val Lys
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Gly Asn Pro Ala Ser Lys Thr His Thr Gly Lys Trp Lys Ala Cys Tyr
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Ser Ile Leu Gly Gly Glu Phe Cys Gly Ala Leu Ala Tyr Tyr Ala Val
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Gly Thr Asn Leu Val Ser Tyr Leu Thr Lys Val Gln Gly Gln Ser Asn
65 70 75 80
Val Thr Ala Ala Ser Asn Ile Ala Ala Trp Gln Gly Asn Cys Tyr Leu
85 90 95
Thr Thr Ile Leu Gly Ala Phe Leu Ala Asp Ser Tyr Trp Gly Arg His
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Arg Thr Ile Val Val Ser Leu Thr Thr Phe Thr Phe Gly Met Val Leu
115 120 125
Leu Thr Leu Ser Ala Val Val Pro Pro Asn Met His Arg Ser Met Ala
130 135 140
Thr Phe Pro Gln Glu Ala Leu Ser Ser Leu Gly Leu Tyr Met Thr Ala
145 150 155 160
Leu Gly Leu Gly Gly Ile Trp Pro Cys Val Pro Thr Phe Gly Ala Asp
165 170 175
Gln Phe Asp Asp Thr Asp Val Ser Glu Lys Ala Gln Lys Glu Leu Phe
180 185 190
Tyr Asn Trp Tyr Tyr Phe Ala Val Asn Gly Gly Phe Phe Val Ala Ser
195 200 205
Thr Val Ile Val Trp Val Gln Asp Asn Cys Gly Trp Gly Leu Gly Phe
210 215 220
Gly Ile Pro Thr Leu Phe Ser Val Ile Gly Val Val Gly Phe Leu Ala
225 230 235 240
Ser Met Arg Phe Tyr Arg Tyr Gln Lys Pro Gly Gly Ser Ala Leu Thr
245 250 255
Arg Ile Cys Gln Val Val Val Ala Ala Phe Arg Lys Val His Val Asp
260 265 270
Val Pro Ser Asp Ser Ser Leu Leu Tyr Glu Met Pro Gly Lys Glu Ser
275 280 285
Ala Ile Val Gly Ser Arg Lys Leu Met His Thr Asp Gly Leu Arg Phe
290 295 300
Phe Asp Arg Ala Ala Thr Ile Thr Ala Ser Asp Glu Ala Ser Ala Ser
305 310 315 320
Arg Pro Trp Lys Leu Cys Thr Val Thr Gln Val Glu Glu Leu Lys Ile
325 330 335
Phe Ala Arg Met Leu Pro Ile Phe Leu Thr Gly Val Ile Phe Asn Thr
340 345 350
Ala Glu Ala Cys Phe Pro Leu Phe Val Glu Gln Gly Gly Ala Met Asp
355 360 365
Asn His Val Ala Ala Ala Phe Ala Leu Pro Pro Ala Ser Leu Thr Thr
370 375 380
Phe Thr Cys Val Cys Ile Leu Val Leu Ala Pro Thr Tyr Asp Arg Val
385 390 395 400
Leu Met Pro Ala Val Ser Arg Leu Thr Gly Val Lys Arg Gly Leu Ser
405 410 415
Glu Leu His Arg Ile Gly Val Gly Met Val Phe Ala Val Leu Ala Leu
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Ala Ala Ala Ala Ala Val Glu Thr Ala Arg Leu Arg Ser Val Glu Ala
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Asp Ala Pro Ala Val Ser Ile Leu Trp Gln Ala Pro Gln Tyr Val Leu
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Ala Ala Leu Gln Leu Ile Asn Leu Ile Ala Phe Val Cys Cys Ala Ala
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atggcaaatg aggagcagcg gcagtggatg gacggggagt ttggctgcca gccggaggaa 60
ggtccataca caggcaatgg atcagttgat gtcaaaggca atccagcatc taaaacacac 120
actgggaaat ggaaggcatg ctactctatt ctaggtggcg aattttgcgg cgcattggcg 180
tactacgcag tagggaccaa cctggtgagc tacctgacca aggtacaggg acagagcaat 240
gtcactgctg ctagcaacat cgctgcttgg cagggtaact gctacctcac cacaatactc 300
ggcgcgttcc tcgcagactc ctattgggga aggcaccgca caatcgtcgt ctccctgacc 360
acctttacct ttggaatggt tctactcaca ctttcagcag tggttccacc aaacatgcac 420
agatcaatgg caacctttcc tcaggaggca ttgtcctctc ttggcctcta catgacagct 480
ctgggcttgg gtggcatatg gccatgtgtg ccgacattcg gagccgatca gttcgacgac 540
accgacgttt cagagaaggc gcagaaggag ctcttctaca actggtacta cttcgctgtc 600
aatggcgggt tcttcgtcgc gagcacggtt atagtgtggg ttcaggataa ctgtggttgg 660
ggactgggtt ttgggatccc tacactgttc tcggtcatcg gcgttgtcgg attccttgcg 720
agtatgaggt tttacaggta ccagaaaccc ggcggtagcg cgcttactag gatctgccag 780
gttgtcgtgg cggcgtttcg caaggtccac gtggacgtgc caagtgacag ctctctgctc 840
tatgagatgc cagggaagga gtctgccatt gttggcagca ggaagctgat gcacactgat 900
ggactcaggt tctttgaccg agctgccact atcactgcat ccgatgaagc atcagctagt 960
cgtccatgga agctgtgcac ggtgacgcag gtggaggagc tcaagatctt tgcgaggatg 1020
ctccccatct tcctcaccgg agtaatcttc aacaccgccg aggcctgctt cccgctgttc 1080
gtcgagcagg gaggcgccat ggacaaccac gtcgccgccg ccttcgcctt gccaccggcg 1140
tcgctgacga ccttcacctg cgtctgcatc ctcgtcctcg cgccgacata cgacagggtc 1200
ctcatgccgg cggtgagcag gctcaccggc gtcaagcgcg gcctctccga gctgcaccgc 1260
atcggcgtcg gaatggtctt cgccgttctc gcgctcgccg ccgccgcggc cgttgagacg 1320
gcgcgcctcc gcagcgtgga ggcggatgct ccggcggtga gcatcctgtg gcaggcgccg 1380
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tacgagcaga gccccgacgc catgaggagc ttgtgccagg cgtgctcgct catcatggtc 1500
acccccggga gctacctcct ctccgccatg ctcaccatca tcagctccgt caccggcggc 1560
ggcggcggcc atggcggatg gatcccggag aatctgaacg agggacatct cgatcgattc 1620
ttctggttga tggcggcgtt gcagctcatc aacctcatcg ccttcgtctg ctgcgctgcc 1680
acctacaaac gcaagctgcc taccacttga 1710
Claims (5)
1.OsNPF8.8bThe application of the gene in improving the tillering number of rice is characterized in that: saidOsNPF8.8bThe amino acid sequence of the OsNPF8.8b protein coded by the gene is shown as SEQ ID NO. 1; the application is realized by improving the expression of the OsNPF8.8b gene.
2.OsNPF8.8bThe application of the gene in improving the effective spike number of rice is characterized in that: saidOsNPF8.8bThe amino acid sequence of the OsNPF8.8b protein coded by the gene is shown as SEQ ID NO. 1; the application is realized by improving the expression of the OsNPF8.8b gene.
3.OsNPF8.8bThe application of the gene in improving the rice yield is characterized in that: saidOsNPF8.8bThe amino acid sequence of the OsNPF8.8b protein coded by the gene is shown as SEQ ID NO. 1; the rice yield is the grain number and dry weight of each single plant of rice seeds; the application is realized by improving the expression of the OsNPF8.8b gene.
4.OsNPF8.8bThe application of the gene in improving the nutritional quality of rice is characterized in that: saidOsNPF8.8bThe amino acid sequence of the OsNPF8.8b protein coded by the gene is shown as SEQ ID NO. 1; the improved rice nutrition quality is that the globulin content in rice is increased; the application is realized by improving the expression of the OsNPF8.8b gene.
5. Use according to any one of claims 1 to 4, characterized in that: saidOsNPF8.8bThe cDNA sequence of the gene is shown in SEQ ID NO. 2.
Priority Applications (1)
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| CN114805515B (en) * | 2022-05-11 | 2023-05-02 | 武汉生物工程学院 | Application of F-box protein coding gene OsFBX250 in rice breeding |
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